Genomes and Genes
Experts and Doctors on saccharomyces cerevisiae proteins in Ithaca, New York, United States
Locale: Ithaca, New York, United States
Topic: saccharomyces cerevisiae proteins
Publications164 found, 100 shown here
- Nishant K, Plys A, Alani E. A mutation in the putative MLH3 endonuclease domain confers a defect in both mismatch repair and meiosis in Saccharomyces cerevisiae. Genetics. 2008;179:747-55 pubmed publisher..These studies suggest that the putative endonuclease domain of MLH3 plays an important role in facilitating mismatch repair and meiotic crossing over. ..
- Bastos de Oliveira F, Harris M, Brazauskas P, de Bruin R, Smolka M. Linking DNA replication checkpoint to MBF cell-cycle transcription reveals a distinct class of G1/S genes. EMBO J. 2012;31:1798-810 pubmed publisher..Our results reveal that replication stress induces expression of G1/S genes via the Rad53-MBF pathway and that an SBF-to-MBF switch characterizes a new class of genes that can be induced by replication stress. ..
- Tang S, Henne W, Borbat P, Buchkovich N, Freed J, Mao Y, et al. Structural basis for activation, assembly and membrane binding of ESCRT-III Snf7 filaments. elife. 2015;4: pubmed publisher..The architecture of the activated and membrane-bound Snf7 polymer provides crucial insights into the spatially unique ESCRT-III-mediated membrane remodeling. ..
- Zheng Y, Cerione R, Bender A. Control of the yeast bud-site assembly GTPase Cdc42. Catalysis of guanine nucleotide exchange by Cdc24 and stimulation of GTPase activity by Bem3. J Biol Chem. 1994;269:2369-72 pubmed..These studies demonstrate that Cdc24 and Bem3 have GDP-release factor activity and GTPase-activating protein activity, respectively, toward the essential bud-site assembly GTPase Cdc42. ..
- Lei M, Kawasaki Y, Young M, Kihara M, Sugino A, Tye B. Mcm2 is a target of regulation by Cdc7-Dbf4 during the initiation of DNA synthesis. Genes Dev. 1997;11:3365-74 pubmed..Taken together, our data suggest that phosphorylation of Mcm2 and probably other members of the Mcm2-7 proteins by Cdc7-Dbf4 at the G1-to-S phase transition is a critical step in the initiation of DNA synthesis at replication origins. ..
- Schott D, Collins R, Bretscher A. Secretory vesicle transport velocity in living cells depends on the myosin-V lever arm length. J Cell Biol. 2002;156:35-9 pubmed..Thus, secretory vesicle polarization is achieved through active transport by a myosin-V, and the motor mechanism is consistent with the lever arm model. ..
- Fitch M, Donato J, Tye B. Mcm7, a subunit of the presumptive MCM helicase, modulates its own expression in conjunction with Mcm1. J Biol Chem. 2003;278:25408-16 pubmed..The multiple roles of Mcm7 in replication initiation, replication elongation, and autoregulation parallel those of the oncoprotein, the large T-antigen of the SV40 virus. ..
- Williams E, Perez Martinez X, Fox T. MrpL36p, a highly diverged L31 ribosomal protein homolog with additional functional domains in Saccharomyces cerevisiae mitochondria. Genetics. 2004;167:65-75 pubmed..We propose that MrpL36p represents a highly diverged L31 homolog with derived domains functioning in mRNA selection in yeast mitochondria. ..
- Manford A, Stefan C, Yuan H, MacGurn J, Emr S. ER-to-plasma membrane tethering proteins regulate cell signaling and ER morphology. Dev Cell. 2012;23:1129-40 pubmed publisher..These results reveal critical roles for ER-PM contacts in cell signaling, organelle morphology, and ER function. ..
- Chen C, Jomaa A, Ortega J, ALANI E. Pch2 is a hexameric ring ATPase that remodels the chromosome axis protein Hop1. Proc Natl Acad Sci U S A. 2014;111:E44-53 pubmed publisher..Based on these and previous cell biological observations, we suggest that Pch2 impacts meiotic chromosome function by directly regulating Hop1 localization...
- Fernandes M, Xiao H, Lis J. Fine structure analyses of the Drosophila and Saccharomyces heat shock factor--heat shock element interactions. Nucleic Acids Res. 1994;22:167-73 pubmed..Finally, based on these observations and a re-evaluation of the base frequencies and criteria for consensus sequence assignment, we propose that the sequence AGAAN more accurately represents the consensus HSE motif. ..
- Bale S, Rajashankar K, Perry K, Begley T, Ealick S. HMP binding protein ThiY and HMP-P synthase THI5 are structural homologues. Biochemistry. 2010;49:8929-36 pubmed publisher..A homology model of Saccharomyces cerevisiae THI5 was generated on the basis of the structure of ThiY. Many features of the thiamin pyrimidine binding site are shared between ThiY and THI5, suggesting a common ancestor. ..
- Basu J, Williams B, Li Z, Williams E, Goldberg M. Depletion of a Drosophila homolog of yeast Sup35p disrupts spindle assembly, chromosome segregation, and cytokinesis during male meiosis. Cell Motil Cytoskeleton. 1998;39:286-302 pubmed..We discuss how depletion of the dsup35 gene product in spermatocytes might lead to the global disruptions in meiotic spindle assembly seen in mutant spermatocytes. ..
- Studamire B, Quach T, Alani E. Saccharomyces cerevisiae Msh2p and Msh6p ATPase activities are both required during mismatch repair. Mol Cell Biol. 1998;18:7590-601 pubmed..Furthermore, our data support a model whereby Msh6p uses its ATP binding or hydrolysis activity to coordinate mismatch binding with additional mismatch repair components. ..
- Homesley L, Lei M, Kawasaki Y, Sawyer S, Christensen T, Tye B. Mcm10 and the MCM2-7 complex interact to initiate DNA synthesis and to release replication factors from origins. Genes Dev. 2000;14:913-26 pubmed..These results suggest that Mcm10, like Mcm7, is a critical component of the prereplication chromatin and that interaction between Mcm10 and Mcm7 is required for proper replication initiation and prompt release of origin-bound factors. ..
- Evans E, Sugawara N, Haber J, Alani E. The Saccharomyces cerevisiae Msh2 mismatch repair protein localizes to recombination intermediates in vivo. Mol Cell. 2000;5:789-99 pubmed..These findings argue that Msh2p-Msh3p associates with intermediates early in DSBR to participate in the rejection of homeologous pairing and to stabilize nonhomologous tails for cleavage by Rad1p-Rad10p endonuclease. ..
- Calero M, Whittaker G, Collins R. Yop1p, the yeast homolog of the polyposis locus protein 1, interacts with Yip1p and negatively regulates cell growth. J Biol Chem. 2001;276:12100-12 pubmed..These results suggest that Yop1p acts in conjunction with Yip1p to mediate a common step in membrane traffic. ..
- Reczek D, Bretscher A. Identification of EPI64, a TBC/rabGAP domain-containing microvillar protein that binds to the first PDZ domain of EBP50 and E3KARP. J Cell Biol. 2001;153:191-206 pubmed..Nadrin binds EBP50 directly, probably through its COOH-terminal STAL sequence. Thus, EBP50 appears to bind membrane proteins as well as factors potentially involved in regulating membrane traffic. ..
- You L, Gillilan R, Huffaker T. Model for the yeast cofactor A-beta-tubulin complex based on computational docking and mutagensis. J Mol Biol. 2004;341:1343-54 pubmed..Rbl2 and alpha-tubulin share overlapping binding sites on the beta-tubulin molecule providing a structural explanation for the mutually exclusive binding of Rbl2 and alpha-tubulin to beta-tubulin. ..
- Gao L, Bretscher A. Analysis of unregulated formin activity reveals how yeast can balance F-actin assembly between different microfilament-based organizations. Mol Biol Cell. 2008;19:1474-84 pubmed publisher..Thus, cells must have a way of ensuring a proper balance between actin assembly pathways. ..
- Rahl P, Chen C, Collins R. Elp1p, the yeast homolog of the FD disease syndrome protein, negatively regulates exocytosis independently of transcriptional elongation. Mol Cell. 2005;17:841-53 pubmed..Our results raise the possibility that regulation of polarized exocytosis is an evolutionarily conserved function of the entire Elongator complex and that FD results from a dysregulation of neuronal exocytosis. ..
- Sonntag Brown M, Lim E, Chen C, Nishant K, Alani E. Genetic analysis of mlh3 mutations reveals interactions between crossover promoting factors during meiosis in baker's yeast. G3 (Bethesda). 2013;3:9-22 pubmed publisher..Together, these observations provide insights for how Mlh1-Mlh3 acts in crossover resolution and MMR and for how chromosome segregation in Meiosis I can occur in the absence of crossing over. ..
- Zhao X, Lis J, Shi H. A systematic study of the features critical for designing a high avidity multivalent aptamer. Nucleic Acid Ther. 2013;23:238-42 pubmed publisher..In particular, we describe the effect of 2 factors in designing a multivalent aptamer: the distance between active subunits and the flexibility of the linkage. ..
- McMullin T, Haffter P, Fox T. A novel small-subunit ribosomal protein of yeast mitochondria that interacts functionally with an mRNA-specific translational activator. Mol Cell Biol. 1990;10:4590-5 pubmed
- Kosco K, Pearson C, Maddox P, Wang P, Adams I, Salmon E, et al. Control of microtubule dynamics by Stu2p is essential for spindle orientation and metaphase chromosome alignment in yeast. Mol Biol Cell. 2001;12:2870-80 pubmed..Overall, we provide evidence that Stu2p promotes the dynamics of microtubule plus-ends in vivo and that these dynamics are critical for microtubule interactions with kinetochores and cortical sites in the cytoplasm. ..
- Christensen T, Tye B. Drosophila MCM10 interacts with members of the prereplication complex and is required for proper chromosome condensation. Mol Biol Cell. 2003;14:2206-15 pubmed..The condensation defects observed resemble previously published reports for Orc2, Orc5, and Mcm4 mutants. Our results strengthen and extend the argument that the processes of chromatin condensation and DNA replication are linked. ..
- Perez Martinez X, Broadley S, Fox T. Mss51p promotes mitochondrial Cox1p synthesis and interacts with newly synthesized Cox1p. EMBO J. 2003;22:5951-61 pubmed..Mss51p was found to interact physically with newly synthesized Cox1p, suggesting that it could coordinate Cox1p synthesis with insertion into the inner membrane or cytochrome oxidase assembly. ..
- Wolyniak M, Blake Hodek K, Kosco K, Hwang E, You L, Huffaker T. The regulation of microtubule dynamics in Saccharomyces cerevisiae by three interacting plus-end tracking proteins. Mol Biol Cell. 2006;17:2789-98 pubmed..These results indicate that interactions among +TIPS can play important roles in the regulation of microtubule dynamics. ..
- Liu W, Santiago Tirado F, Bretscher A. Yeast formin Bni1p has multiple localization regions that function in polarized growth and spindle orientation. Mol Biol Cell. 2012;23:412-22 pubmed publisher..These results define an unexpected complexity in the mechanism of formin localization and function. ..
- Al Sweel N, Raghavan V, Dutta A, Ajith V, Di Vietro L, Khondakar N, et al. mlh3 mutations in baker's yeast alter meiotic recombination outcomes by increasing noncrossover events genome-wide. PLoS Genet. 2017;13:e1006974 pubmed publisher..They also illustrate how defective meiotic components can alter the fate of meiotic recombination intermediates, providing new insights for how meiotic recombination pathways are regulated. ..
- Haffter P, Fox T. Suppression of carboxy-terminal truncations of the yeast mitochondrial mRNA-specific translational activator PET122 by mutations in two new genes, MRP17 and PET127. Mol Gen Genet. 1992;235:64-73 pubmed..These findings suggest the possibility that PET127 could be a novel component of the mitochondrial translation system with a role in promoting accuracy of translational initiation. ..
- Mulero J, Fox T. Alteration of the Saccharomyces cerevisiae COX2 mRNA 5'-untranslated leader by mitochondrial gene replacement and functional interaction with the translational activator protein PET111. Mol Biol Cell. 1993;4:1327-35 pubmed..These results demonstrate an intimate functional interaction between the translational activator protein and the mRNA 5'-UTL and suggest that they may interact directly. ..
- Pruyne D, Bretscher A. Polarization of cell growth in yeast. I. Establishment and maintenance of polarity states. J Cell Sci. 2000;113 ( Pt 3):365-75 pubmed..Given that many of these signaling pathways are present in both fungi and animals, they are probably ancient and conserved mechanisms for regulating polarity. ..
- Green Willms N, Butler C, Dunstan H, Fox T. Pet111p, an inner membrane-bound translational activator that limits expression of the Saccharomyces cerevisiae mitochondrial gene COX2. J Biol Chem. 2001;276:6392-7 pubmed..8-fold in a strain bearing extra copies of PET111 on a high-copy plasmid. Thus, Pet111p could play dual roles in both membrane localization and regulation of Cox2p synthesis within mitochondria. ..
- Fiori A, Perez Martinez X, Fox T. Overexpression of the COX2 translational activator, Pet111p, prevents translation of COX1 mRNA and cytochrome c oxidase assembly in mitochondria of Saccharomyces cerevisiae. Mol Microbiol. 2005;56:1689-704 pubmed
- Williams E, Butler C, Bonnefoy N, Fox T. Translation initiation in Saccharomyces cerevisiae mitochondria: functional interactions among mitochondrial ribosomal protein Rsm28p, initiation factor 2, methionyl-tRNA-formyltransferase and novel protein Rmd9p. Genetics. 2007;175:1117-26 pubmed
- Plys A, Rogacheva M, Greene E, Alani E. The unstructured linker arms of Mlh1-Pms1 are important for interactions with DNA during mismatch repair. J Mol Biol. 2012;422:192-203 pubmed publisher..These observations are consistent with the unstructured linker domains of MLH proteins providing distinct interactions with DNA during MMR. ..
- Das J, Yu H. HINT: High-quality protein interactomes and their applications in understanding human disease. BMC Syst Biol. 2012;6:92 pubmed publisher..These datasets can be utilized to generate specific hypotheses about specific proteins and/or pathways, as well as analyzing global properties of cellular networks. HINT will be regularly updated and all versions will be tracked. ..
- Zheng Y, Hart M, Shinjo K, Evans T, Bender A, Cerione R. Biochemical comparisons of the Saccharomyces cerevisiae Bem2 and Bem3 proteins. Delineation of a limit Cdc42 GTPase-activating protein domain. J Biol Chem. 1993;268:24629-34 pubmed..quot; The first two appear to be important for binding to Cdc42Hs and for partial GAP activity. ..
- Kopski K, Huffaker T. Suppressors of the ndc10-2 mutation: a role for the ubiquitin system in Saccharomyces cerevisiae kinetochore function. Genetics. 1997;147:409-20 pubmed..Furthermore, overexpression of a mutant ubiquitin suppresses the ndc10-2 mutation. These results implicate the ubiquitin system in the regulation of ndc10-2 function and suggest a role for the ubiquitin system in kinetochore function. ..
- Nilsson R, van Wijk K. Transient interaction of cpSRP54 with elongating nascent chains of the chloroplast-encoded D1 protein; 'cpSRP54 caught in the act'. FEBS Lett. 2002;524:127-33 pubmed..Detailed mutant analysis showed that the cpSRP54 interaction occurred via the first transmembrane domain, which could be replaced by other hydrophobic domains of more than 10 amino acids. ..
- Cheung E, Schwabish M, Kraus W. Chromatin exposes intrinsic differences in the transcriptional activities of estrogen receptors alpha and beta. EMBO J. 2003;22:600-11 pubmed..g. with an open chromatin architecture). Collectively, our results define an important role for chromatin in determining signaling outcomes mediated by distinct subtypes of signal-transducing transcriptional activator proteins. ..
- Hwang E, Kusch J, Barral Y, Huffaker T. Spindle orientation in Saccharomyces cerevisiae depends on the transport of microtubule ends along polarized actin cables. J Cell Biol. 2003;161:483-8 pubmed..We also present data suggesting that a similar process plays a role in orienting cytoplasmic microtubules in mating yeast cells. ..
- Chen C, Calero M, DeRegis C, Heidtman M, Barlowe C, Collins R. Genetic analysis of yeast Yip1p function reveals a requirement for Golgi-localized rab proteins and rab-Guanine nucleotide dissociation inhibitor. Genetics. 2004;168:1827-41 pubmed
- Heck J, Argueso J, Gemici Z, Reeves R, Bernard A, Aquadro C, et al. Negative epistasis between natural variants of the Saccharomyces cerevisiae MLH1 and PMS1 genes results in a defect in mismatch repair. Proc Natl Acad Sci U S A. 2006;103:3256-61 pubmed..This same segregational mutator phenotype suggests a mechanism to explain some cases of a human cancer susceptibility syndrome known as hereditary nonpolyposis colorectal cancer, as well as some sporadic cancers. ..
- Shi Y, Stefan C, Rue S, Teis D, Emr S. Two novel WD40 domain-containing proteins, Ere1 and Ere2, function in the retromer-mediated endosomal recycling pathway. Mol Biol Cell. 2011;22:4093-107 pubmed publisher..Moreover, a complex containing the two novel proteins Ere1 and Ere2 mediates cargo-specific recognition by the retromer pathway. ..
- Omnus D, Manford A, Bader J, Emr S, Stefan C. Phosphoinositide kinase signaling controls ER-PM cross-talk. Mol Biol Cell. 2016;27:1170-80 pubmed publisher..Thus PI kinase-mediated ER-PM cross-talk comprises a regulatory system that ensures cellular integrity under membrane stress conditions. ..
- Yan H, Gibson S, Tye B. Mcm2 and Mcm3, two proteins important for ARS activity, are related in structure and function. Genes Dev. 1991;5:944-57 pubmed..cerevisiae, and Nda4 and Cdc21 of Schizosaccharomyces pombe. The phenotypes of mutants defective in these proteins suggest that they belong to a protein family involved in the early steps of DNA replication. ..
- Schott D, Ho J, Pruyne D, Bretscher A. The COOH-terminal domain of Myo2p, a yeast myosin V, has a direct role in secretory vesicle targeting. J Cell Biol. 1999;147:791-808 pubmed..The data support a model in which the Myo2p tail tethers secretory vesicles, and the motor transports them down polarized actin cables to the site of exocytosis. ..
- Jesch S, Zhao X, Wells M, Henry S. Genome-wide analysis reveals inositol, not choline, as the major effector of Ino2p-Ino4p and unfolded protein response target gene expression in yeast. J Biol Chem. 2005;280:9106-18 pubmed..These results suggest that inositol is the major effector of target gene expression, whereas choline plays a minor role. ..
- Jurgenson C, Chatterjee A, Begley T, Ealick S. Structural insights into the function of the thiamin biosynthetic enzyme Thi4 from Saccharomyces cerevisiae. Biochemistry. 2006;45:11061-70 pubmed..Additionally, the Thi4 structure reveals the first protein structure with a GR(2) domain that binds NAD instead of FAD, raising interesting questions about how this protein evolved from a flavoenzyme to a NAD binding enzyme. ..
- Huang B, Huffaker T. Dynamic microtubules are essential for efficient chromosome capture and biorientation in S. cerevisiae. J Cell Biol. 2006;175:17-23 pubmed
- Li X, Tye B. Ploidy dictates repair pathway choice under DNA replication stress. Genetics. 2011;187:1031-40 pubmed publisher..In response to replication stress, early events associated with ploidy dictate the repair pathway choice. This study uncovers a fundamental difference between haplophase and diplophase in the maintenance of genome integrity. ..
- Zanders S, Sonntag Brown M, Chen C, Alani E. Pch2 modulates chromatid partner choice during meiotic double-strand break repair in Saccharomyces cerevisiae. Genetics. 2011;188:511-21 pubmed publisher..We propose a model in which Pch2 is required to promote full Mek1 activity and thereby promotes interhomolog repair...
- Sevier C. Erv2 and quiescin sulfhydryl oxidases: Erv-domain enzymes associated with the secretory pathway. Antioxid Redox Signal. 2012;16:800-8 pubmed publisher..Also, determining when Erv2 and QSOX function during growth and development, and how changes in levels of active Erv2 and QSOX impact cell function, is necessary to facilitate a better understanding of these intriguing enzymes. ..
- Valderrama Rincon J, Fisher A, Merritt J, Fan Y, Reading C, Chhiba K, et al. An engineered eukaryotic protein glycosylation pathway in Escherichia coli. Nat Chem Biol. 2012;8:434-6 pubmed publisher..By including the bacterial oligosaccharyltransferase PglB from Campylobacter jejuni, we successfully transferred glycans to eukaryotic proteins. ..
- Henne W, Buchkovich N, Zhao Y, Emr S. The endosomal sorting complex ESCRT-II mediates the assembly and architecture of ESCRT-III helices. Cell. 2012;151:356-71 pubmed publisher..We propose that ESCRT-III has distinct architectural stages that are modulated by ESCRT-II to mediate cargo capture and vesicle formation by ordered assembly. ..
- Ohouo P, Bastos de Oliveira F, Liu Y, Ma C, Smolka M. DNA-repair scaffolds dampen checkpoint signalling by counteracting the adaptor Rad9. Nature. 2013;493:120-4 pubmed publisher..Our findings show that DDC signalling is monitored and modulated through the direct action of DNA-repair factors. ..
- Hofbauer H, Schopf F, Schleifer H, Knittelfelder O, Pieber B, Rechberger G, et al. Regulation of gene expression through a transcriptional repressor that senses acyl-chain length in membrane phospholipids. Dev Cell. 2014;29:729-39 pubmed publisher..These findings reveal an unexpected regulatory link between the major energy-sensing kinase, membrane lipid composition, and transcription. ..
- Broadley S, Demlow C, Fox T. Peripheral mitochondrial inner membrane protein, Mss2p, required for export of the mitochondrially coded Cox2p C tail in Saccharomyces cerevisiae. Mol Cell Biol. 2001;21:7663-72 pubmed..Taken together, these data indicate that Mss2p plays a role in recognizing the Cox2p C tail in the matrix and promoting its export. ..
- Kang S, Hoke K, Crane B. Solvent isotope effects on interfacial protein electron transfer in crystals and electrode films. J Am Chem Soc. 2006;128:2346-55 pubmed..Thus, D(2)O substitution perturbs interactions and ET between yCc and either CcP or electrode films. The effects derive from slow exchanging protons or solvent molecules that in the crystal produce only small structural changes. ..
- Surtees J, Alani E. Mismatch repair factor MSH2-MSH3 binds and alters the conformation of branched DNA structures predicted to form during genetic recombination. J Mol Biol. 2006;360:523-36 pubmed..Therefore, MSH2-MSH3 binding to its substrates creates a unique nucleoprotein structure that may signal downstream steps in repair that include interactions with MMR and nucleotide excision repair factors. ..
- Rogacheva M, Manhart C, Chen C, Guarné A, SURTEES J, Alani E. Mlh1-Mlh3, a meiotic crossover and DNA mismatch repair factor, is a Msh2-Msh3-stimulated endonuclease. J Biol Chem. 2014;289:5664-73 pubmed publisher..These observations support a direct role for an Mlh1-Mlh3 endonuclease activity in resolving recombination intermediates and in DNA mismatch repair. ..
- Ho H, MacGurn J, Emr S. Deubiquitinating enzymes Ubp2 and Ubp15 regulate endocytosis by limiting ubiquitination and degradation of ARTs. Mol Biol Cell. 2017;28:1271-1283 pubmed publisher..Our results uncover a novel function for DUBs in the endocytic pathway by which Ubp2 and Ubp15 positively regulate the ART-Rsp5 network. ..
- Dong Y, Pruyne D, Bretscher A. Formin-dependent actin assembly is regulated by distinct modes of Rho signaling in yeast. J Cell Biol. 2003;161:1081-92 pubmed..These results show that formin function is under the control of three distinct, essential Rho signaling pathways. ..
- Giardina C, Lis J. Dynamic protein-DNA architecture of a yeast heat shock promoter. Mol Cell Biol. 1995;15:2737-44 pubmed
- Chen R. BubR1 is essential for kinetochore localization of other spindle checkpoint proteins and its phosphorylation requires Mad1. J Cell Biol. 2002;158:487-96 pubmed..This paper demonstrates that BubR1 plays an important role in kinetochore association of other spindle checkpoint proteins and that Mad1 facilitates BubR1 hyperphosphorylation at kinetochores. ..
- Williams E, Bsat N, Bonnefoy N, Butler C, Fox T. Alteration of a novel dispensable mitochondrial ribosomal small-subunit protein, Rsm28p, allows translation of defective COX2 mRNAs. Eukaryot Cell. 2005;4:337-45 pubmed..We conclude that Rsm28p is a dispensable small-subunit mitochondrial ribosomal protein previously undetected in systematic investigations of these ribosomes, with a positive role in translation of several mitochondrial mRNAs. ..
- Chakraborty U, George C, Lyndaker A, Alani E. A Delicate Balance Between Repair and Replication Factors Regulates Recombination Between Divergent DNA Sequences in Saccharomyces cerevisiae. Genetics. 2016;202:525-40 pubmed publisher..These observations illustrate the delicate balance that exists between repair and replication factors to optimize genome stability. ..
- Yin H, You L, Pasqualone D, Kopski K, Huffaker T. Stu1p is physically associated with beta-tubulin and is required for structural integrity of the mitotic spindle. Mol Biol Cell. 2002;13:1881-92 pubmed..Taken together, these findings suggest that Stu1p binds to interpolar microtubules of the mitotic spindle and plays an essential role in their ability to provide an outward force on the spindle poles. ..
- Sawyer S, Cheng I, Chai W, Tye B. Mcm10 and Cdc45 cooperate in origin activation in Saccharomyces cerevisiae. J Mol Biol. 2004;340:195-202 pubmed..Our results show that interaction between the Mcm10 and Cdc45 proteins facilitates the recruitment of Cdc45 onto the ARS1 origin. ..
- Buchkovich N, Henne W, Tang S, Emr S. Essential N-terminal insertion motif anchors the ESCRT-III filament during MVB vesicle formation. Dev Cell. 2013;27:201-214 pubmed publisher..Our results provide insights into the spatially unique, ESCRT-III-mediated membrane remodeling. ..
- Wang P, Chabes A, Casagrande R, Tian X, Thelander L, Huffaker T. Rnr4p, a novel ribonucleotide reductase small-subunit protein. Mol Cell Biol. 1997;17:6114-21 pubmed..These results suggest that Rnr4p is catalytically inactive but, nonetheless, does play a role in the ribonucleotide reductase complex. ..
- Williams B, Garrett Engele C, Li Z, Williams E, Rosenman E, Goldberg M. Two putative acetyltransferases, san and deco, are required for establishing sister chromatid cohesion in Drosophila. Curr Biol. 2003;13:2025-36 pubmed..At least two diverse acetyltransferases play vital roles in regulating sister chromatid cohesion during Drosophila mitosis. ..
- Wang T, Bretscher A. Mutations synthetically lethal with tpm1delta lie in genes involved in morphogenesis. Genetics. 1997;147:1595-607 pubmed..These results indicate that this synthetic lethality approach provides a sensitive screen for the isolation of mutations affecting morphogenesis, many of which are likely to be in nonessential genes, like BEM2 and SLT2. ..
- Lee S, Surtees J, Alani E. Saccharomyces cerevisiae MSH2-MSH3 and MSH2-MSH6 complexes display distinct requirements for DNA binding domain I in mismatch recognition. J Mol Biol. 2007;366:53-66 pubmed
- Manford A, Xia T, Saxena A, Stefan C, Hu F, Emr S, et al. Crystal structure of the yeast Sac1: implications for its phosphoinositide phosphatase function. EMBO J. 2010;29:1489-98 pubmed publisher..Homology structural modeling of human Fig4/Sac3 allows the mapping of several disease-related mutations and provides a framework for the understanding of the molecular mechanisms of human diseases. ..
- Lei M, Kawasaki Y, Tye B. Physical interactions among Mcm proteins and effects of Mcm dosage on DNA replication in Saccharomyces cerevisiae. Mol Cell Biol. 1996;16:5081-90 pubmed..These results together suggest that a significant molar excess of Mcm proteins relative to replication origins is required for the proper initiation of all replication origins. ..
- Alani E, Lee S, Kane M, Griffith J, Kolodner R. Saccharomyces cerevisiae MSH2, a mispaired base recognition protein, also recognizes Holliday junctions in DNA. J Mol Biol. 1997;265:289-301 pubmed..This observation suggests that MSH2 protein could directly coordinate the interaction between mismatch repair and genetic recombination observed in genetic studies. ..
- Saracco S, Fox T. Cox18p is required for export of the mitochondrially encoded Saccharomyces cerevisiae Cox2p C-tail and interacts with Pnt1p and Mss2p in the inner membrane. Mol Biol Cell. 2002;13:1122-31 pubmed..The inner membrane proteins Mss2p and Pnt1p both coimmunoprecipitate with Cox18p, suggesting that they work together in translocation of Cox2p domains, an inference supported by functional interactions among the three genes. ..
- Bowers J, Sokolsky T, Quach T, Alani E. A mutation in the MSH6 subunit of the Saccharomyces cerevisiae MSH2-MSH6 complex disrupts mismatch recognition. J Biol Chem. 1999;274:16115-25 pubmed..These observations, in conjunction with ATPase and dissociation rate analysis, suggested that MSH2-msh6-F337A formed an unproductive complex that was unable to stably bind to mismatch DNA. ..
- Argueso J, Wanat J, Gemici Z, Alani E. Competing crossover pathways act during meiosis in Saccharomyces cerevisiae. Genetics. 2004;168:1805-16 pubmed..A third pathway, which appears to be repressed by MSH4-MSH5, yields deleterious crossovers. ..
- Jesch S, Liu P, Zhao X, Wells M, Henry S. Multiple endoplasmic reticulum-to-nucleus signaling pathways coordinate phospholipid metabolism with gene expression by distinct mechanisms. J Biol Chem. 2006;281:24070-83 pubmed..We propose that these signals are generated in the ER by increased flux through the pathway of phosphatidylinositol synthesis. ..
- Baird D, Stefan C, Audhya A, Weys S, Emr S. Assembly of the PtdIns 4-kinase Stt4 complex at the plasma membrane requires Ypp1 and Efr3. J Cell Biol. 2008;183:1061-74 pubmed publisher..Efr3 is essential for assembly of both Ypp1 and Stt4 at PIK patches. We conclude that Ypp1 and Efr3 are required for the formation and architecture of Stt4 PIK patches and ultimately PM-based PtdIns4P signaling. ..