Genomes and Genes
Experts and Doctors on saccharomyces cerevisiae in Piscataway, New Jersey, United States
Locale: Piscataway, New Jersey, United States
Topic: saccharomyces cerevisiae
- Mitchell A, Martin C. Fah1p, a Saccharomyces cerevisiae cytochrome b5 fusion protein, and its Arabidopsis thaliana homolog that lacks the cytochrome b5 domain both function in the alpha-hydroxylation of sphingolipid-associated very long chain fatty acids. J Biol Chem. 1997;272:28281-8 pubmed
- Hanlon S, Xu Z, Norris D, Vershon A. Analysis of the meiotic role of the mitochondrial ribosomal proteins Mrps17 and Mrpl37 in Saccharomyces cerevisiae. Yeast. 2004;21:1241-52 pubmed..Interestingly, we found that Mrps17 and Mrpl37, but not other mitochondrial ribosomal proteins, accumulate during the middle stages of sporulation. These results suggest that Mrps17 and Mrpl37 may have additional meiosis-specific roles. ..
- Zhang X, Schwer B. Functional and physical interaction between the yeast splicing factors Slu7 and Prp18. Nucleic Acids Res. 1997;25:2146-52 pubmed..Deleted versions of Slu7 were also tested for interaction with Prp18 in the two-hybrid system. We define a segment of Slu7 from residue 200 to 224 that is necessary for interaction with Prp18. ..
- Chuang S, Madura K. Saccharomyces cerevisiae Ub-conjugating enzyme Ubc4 binds the proteasome in the presence of translationally damaged proteins. Genetics. 2005;171:1477-84 pubmed..Collectively, these studies suggest a specific role for Ubc4 and Ubc5 in the degradation of cotranslationally damaged proteins that are targeted to the proteasome. ..
- Ortiz P, Ulloque R, Kihara G, Zheng H, Kinzy T. Translation elongation factor 2 anticodon mimicry domain mutants affect fidelity and diphtheria toxin resistance. J Biol Chem. 2006;281:32639-48 pubmed
- Estrella L, Krishnamurthy S, Timme C, Hampsey M. The Rsp5 E3 ligase mediates turnover of low affinity phosphate transporters in Saccharomyces cerevisiae. J Biol Chem. 2008;283:5327-34 pubmed publisher..We conclude that turnover of the low affinity P i transporters is initiated by Rsp5-mediated ubiquitination followed by internalization and degradation by the endocytic pathway. ..
- Schwer B, Shuman S. Multicopy suppressors of temperature-sensitive mutations of yeast mRNA capping enzyme. Gene Expr. 1996;5:331-44 pubmed..CES3 corresponds to BUD5, which encodes a putative guanine nucleotide exchange factor. We hypothesize that CES1, CES4, and BUD5 may impact on RNA transactions downstream of cap synthesis that are cap dependent in vivo. ..
- Bockhorn J, Balar B, He D, Seitomer E, Copeland P, Kinzy T. Genome-wide screen of Saccharomyces cerevisiae null allele strains identifies genes involved in selenomethionine resistance. Proc Natl Acad Sci U S A. 2008;105:17682-7 pubmed publisher..The CYS3 deletion strain provides advantages for an easy and cost-effective method to prepare SeMet-substituted protein in yeast and perhaps other eukaryotic systems. ..
- Mateyak M, Kinzy T. eEF1A: thinking outside the ribosome. J Biol Chem. 2010;285:21209-13 pubmed publisher..A mechanistic understanding of these non-canonical functions of eEF1A will shed light on many important biological questions, including viral-host interaction, subcellular organization, and the integration of key cellular pathways. ..
- Wu C, Chen Y, Gartenberg M. Targeted sister chromatid cohesion by Sir2. PLoS Genet. 2011;7:e1002000 pubmed publisher..Taken together, these data define a unique role for Sir2 in cohesion of silent chromatin that is distinct from the enzyme's role as a histone deacetylase. ..
- Yan Y, Tao Y, Chen K. Molecular cloning and functional expression of human deoxyhypusine synthase cDNA based on expressed sequence tag information. Biochem J. 1996;315 ( Pt 2):429-34 pubmed..After cloning into an expression vector, the 368-amino-acid recombinant protein exhibits high deoxyhypusine synthase activity. In contrast, the 321-amino-acid recombinant protein shows no detectable activity. ..
- Tolkunov D, Zawadzki K, Singer C, Elfving N, Morozov A, Broach J. Chromatin remodelers clear nucleosomes from intrinsically unfavorable sites to establish nucleosome-depleted regions at promoters. Mol Biol Cell. 2011;22:2106-18 pubmed publisher..In summary, active remodeling is required for distributing nucleosomes to energetically favorable positions in vivo and for reorganizing chromatin in response to changes in transcriptional activity. ..
- Zhang S, Ruiz Echevarria M, Quan Y, Peltz S. Identification and characterization of a sequence motif involved in nonsense-mediated mRNA decay. Mol Cell Biol. 1995;15:2231-44 pubmed..This sequence motif is one of the few elements that have been identified that can predict regions in genes that can be involved in mRNA turnover. The role of these sequences in mRNA decay is discussed. ..
- Daroui P, Desai S, Li T, Liu A, Liu L. Hydrogen peroxide induces topoisomerase I-mediated DNA damage and cell death. J Biol Chem. 2004;279:14587-94 pubmed..These results support a model in which hydrogen peroxide promotes the trapping of TOP1 on oxidative DNA lesions to form TOP1-DNA cleavage complexes that contribute to hydrogen peroxide toxicity. ..
- Chereji R, Bharatula V, Elfving N, Blomberg J, Larsson M, Morozov A, et al. Mediator binds to boundaries of chromosomal interaction domains and to proteins involved in DNA looping, RNA metabolism, chromatin remodeling, and actin assembly. Nucleic Acids Res. 2017;45:8806-8821 pubmed publisher..This suggests that Mediator plays a significant role in higher-order genome organization. ..
- Kaliraman V, Mullen J, Fricke W, Bastin Shanower S, Brill S. Functional overlap between Sgs1-Top3 and the Mms4-Mus81 endonuclease. Genes Dev. 2001;15:2730-40 pubmed..Repair of this double-strand break (DSB) by homologous recombination may be responsible for the elevated levels of sister chromatid exchange (SCE) found in BLM(-/-) cells. ..
- Tan Q, Prysak M, Woychik N. Loss of the Rpb4/Rpb7 subcomplex in a mutant form of the Rpb6 subunit shared by RNA polymerases I, II, and III. Mol Cell Biol. 2003;23:3329-38 pubmed..The association of Rpb4/Rpb7 with Rpb6 suggests that analogous subunits of each RNA polymerase impart class-specific functions through a conserved core subunit. ..
- Gartenberg M. The Sir proteins of Saccharomyces cerevisiae: mediators of transcriptional silencing and much more. Curr Opin Microbiol. 2000;3:132-7 pubmed
- Romero Perez L, Chen L, Lambertson D, Madura K. Sts1 can overcome the loss of Rad23 and Rpn10 and represents a novel regulator of the ubiquitin/proteasome pathway. J Biol Chem. 2007;282:35574-82 pubmed..Despite these proteolytic defects, overall proteasome activity was increased in sts1-2. We propose that Sts1 is a new regulatory factor in the ubiquitin/proteasome pathway that controls the turnover of proteasome substrates...
- Dinman J, Ruiz Echevarria M, Czaplinski K, Peltz S. Peptidyl-transferase inhibitors have antiviral properties by altering programmed -1 ribosomal frameshifting efficiencies: development of model systems. Proc Natl Acad Sci U S A. 1997;94:6606-11 pubmed..Our results offer a new set of antiviral agents that may potentially have a broad range of applications in the clinical, veterinary, and agricultural fields. ..
- Maniar H, Wilson R, Brill S. Roles of replication protein-A subunits 2 and 3 in DNA replication fork movement in Saccharomyces cerevisiae. Genetics. 1997;145:891-902 pubmed..Thus, replication fork movement in vivo requires the association of at least two subunits of RPA. This result is consistent with the hypothesis that RPA functions in vivo by binding ssDNA in a higher-order mode. ..
- Benni M, Neigeborn L. Identification of a new class of negative regulators affecting sporulation-specific gene expression in yeast. Genetics. 1997;147:1351-66 pubmed..These phenotypes and interactions with RAS2-Val19 suggest that unscheduled derepression of IME1 is probably due to a defect in recognition of nutritional status. ..
- Anand M, Balar B, Ulloque R, Gross S, Kinzy T. Domain and nucleotide dependence of the interaction between Saccharomyces cerevisiae translation elongation factors 3 and 1A. J Biol Chem. 2006;281:32318-26 pubmed..The dynamics of when eEF3 interacts with eEF1A may be part of the signal for transition of the post to pre-translocational ribosomal state in yeast. ..
- Reyes Reyes M, Hampsey M. Role for the Ssu72 C-terminal domain phosphatase in RNA polymerase II transcription elongation. Mol Cell Biol. 2007;27:926-36 pubmed
- Carr Schmid A, Pfund C, Craig E, Kinzy T. Novel G-protein complex whose requirement is linked to the translational status of the cell. Mol Cell Biol. 2002;22:2564-74 pubmed..Thus, Hbs1p and Dom34p may function together to help directly or indirectly facilitate the expression either of specific mRNAs or under certain cellular conditions. ..
- Ii T, Fung J, Mullen J, Brill S. The yeast Slx5-Slx8 DNA integrity complex displays ubiquitin ligase activity. Cell Cycle. 2007;6:2800-9 pubmed..We propose that the Slx5-Slx8 complex functions as a two-component Ub ligase in vivo and that it controls genome stability and sumoylation via ubiquitination. ..
- Chen L, Madura K. Centrin/Cdc31 is a novel regulator of protein degradation. Mol Cell Biol. 2008;28:1829-40 pubmed..These findings reveal for the first time a new role for centrin/Cdc31 in protein degradation. ..
- Fricke W, Kaliraman V, Brill S. Mapping the DNA topoisomerase III binding domain of the Sgs1 DNA helicase. J Biol Chem. 2001;276:8848-55 pubmed..Thus, a primary role of the Sgs1 amino terminus is to mediate the Top3 interaction. ..
- Ruiz Echevarria M, Peltz S. The RNA binding protein Pub1 modulates the stability of transcripts containing upstream open reading frames. Cell. 2000;101:741-51 pubmed..These results indicate that uORFs can play a pivotal role regulating both translation and turnover and that the Pub1p is a critical factor that modulates the stability of uORF-containing transcripts. ..
- Chereji R, Morozov A. Ubiquitous nucleosome crowding in the yeast genome. Proc Natl Acad Sci U S A. 2014;111:5236-41 pubmed publisher..We rule out several alternative scenarios of histone-DNA interactions as inconsistent with the genomic data. ..
- Jiao X, Xiang S, Oh C, Martin C, Tong L, Kiledjian M. Identification of a quality-control mechanism for mRNA 5'-end capping. Nature. 2010;467:608-11 pubmed publisher..We propose that Rai1 is involved in an as yet uncharacterized quality control process that ensures mRNA 5'-end integrity by an aberrant-cap-mediated mRNA decay mechanism. ..
- Muthukumar G, Suhng S, Magee P, Jewell R, Primerano D. The Saccharomyces cerevisiae SPR1 gene encodes a sporulation-specific exo-1,3-beta-glucanase which contributes to ascospore thermoresistance. J Bacteriol. 1993;175:386-94 pubmed..These observations are consistent with the notion that SPR1 encodes a sporulation-specific exo-1,3-beta-glucanase. ..
- Wei Y, Tsang C, Zheng X. Mechanisms of regulation of RNA polymerase III-dependent transcription by TORC1. EMBO J. 2009;28:2220-30 pubmed publisher..Together, these observations provide new insights into the chromatin-dependent mechanism by which TORC1 controls transcription by Pol III. ..
- Chen C, Brill S. An essential DNA strand-exchange activity is conserved in the divergent N-termini of BLM orthologs. EMBO J. 2010;29:1713-25 pubmed publisher..This work identifies SE as a new molecular function of BLM/Sgs1, and we propose that at least one role of SE is to mediate the strand-passage events catalysed by Top3-Rmi1. ..
- Lee K, Du C, Horn M, Rabinow L. Activity and autophosphorylation of LAMMER protein kinases. J Biol Chem. 1996;271:27299-303 pubmed
- Zhang M, Yu X, Xu Y, Jouhten P, Swapna G, Glaser R, et al. 13 C metabolic flux profiling of Pichia pastoris grown in aerobic batch cultures on glucose revealed high relative anabolic use of TCA cycle and limited incorporation of provided precursors of branched-chain amino acids. FEBS J. 2017;284:3100-3113 pubmed publisher..4.1.1), pyruvate kinase (EC 220.127.116.11), l-serine hydroxymethyltransferase (EC 18.104.22.168), threonine aldolase (EC 22.214.171.124), threonine dehydratase (EC 126.96.36.199); transketolase (EC 188.8.131.52), transaldolase (EC 184.108.40.206). ..
- Mullen J, Das M, Brill S. Genetic evidence that polysumoylation bypasses the need for a SUMO-targeted Ub ligase. Genetics. 2011;187:73-87 pubmed publisher..In support of this latter possibility we find that the WSS1 isopeptidase is required for suppression by ulp2?. ..
- Olarewaju O, Ortiz P, Chowdhury W, Chatterjee I, Kinzy T. The translation elongation factor eEF1B plays a role in the oxidative stress response pathway. RNA Biol. 2004;1:89-94 pubmed..As nucleotide exchange is a critical regulator of most G-proteins, these results indicate a new mechanism in the growing list of examples of post-transcriptional responses to cellular stress. ..
- Tsui K, Simon L, Norris D. Progression into the first meiotic division is sensitive to histone H2A-H2B dimer concentration in Saccharomyces cerevisiae. Genetics. 1997;145:647-59 pubmed..We hypothesize that the deposition of H2A-H2B dimers in the mutant is unable to keep pace with the replication fork, thereby leading to a disruption in chromosome structure that interferes with the meiotic divisions. ..
- Chatterjee I, Gross S, Kinzy T, Chen K. Rapid depletion of mutant eukaryotic initiation factor 5A at restrictive temperature reveals connections to actin cytoskeleton and cell cycle progression. Mol Genet Genomics. 2006;275:264-76 pubmed..Sorbitol, an osmotic stabilizer that complement defects in PKC/WSC pathways, stabilizes the mutant eIF5A and suppresses all the observed temperature-sensitive phenotypes. ..
- Mead J, Zhong H, Acton T, Vershon A. The yeast alpha2 and Mcm1 proteins interact through a region similar to a motif found in homeodomain proteins of higher eukaryotes. Mol Cell Biol. 1996;16:2135-43 pubmed..These findings suggest that homeodomain proteins from diverse organisms may use analogous interaction motifs to associate with other proteins to achieve high levels of DNA binding affinity and specificity. ..
- Ii M, Brill S. Roles of SGS1, MUS81, and RAD51 in the repair of lagging-strand replication defects in Saccharomyces cerevisiae. Curr Genet. 2005;48:213-25 pubmed..These data support a model in which defects in lagging-strand replication are repaired by the Mus81 endonuclease or through a pathway dependent on Rad51 and Sgs1. ..
- Lee J, Godon C, Lagniel G, Spector D, Garin J, Labarre J, et al. Yap1 and Skn7 control two specialized oxidative stress response regulons in yeast. J Biol Chem. 1999;274:16040-6 pubmed..Such a specialization may explain, at least in part, the dissociated function of Yap1 and Skn7 in H2O2 and cadmium resistance. ..
- Pierce M, Benjamin K, Montano S, Georgiadis M, Winter E, Vershon A. Sum1 and Ndt80 proteins compete for binding to middle sporulation element sequences that control meiotic gene expression. Mol Cell Biol. 2003;23:4814-25 pubmed..Our results provide a mechanism for differentially regulating the expression of middle sporulation genes through the competition between the Sum1 repressor and the Ndt80 activator. ..
- Goel S, Krishnamurthy S, Hampsey M. Mechanism of start site selection by RNA polymerase II: interplay between TFIIB and Ssl2/XPB helicase subunit of TFIIH. J Biol Chem. 2012;287:557-67 pubmed publisher..These results define a novel, functional interaction between TFIIB and Ssl2 that affects start site selection and gene looping. ..
- He B, Chen P, Chen S, Vancura K, Michaelis S, Powers S. RAM2, an essential gene of yeast, and RAM1 encode the two polypeptide components of the farnesyltransferase that prenylates a-factor and Ras proteins. Proc Natl Acad Sci U S A. 1991;88:11373-7 pubmed..In contrast, disruption of RAM2 was lethal, suggesting that Ram2 has an essential function in addition to its role with Ram1 in protein farnesylation. ..
- Miyao T, Woychik N. RNA polymerase subunit RPB5 plays a role in transcriptional activation. Proc Natl Acad Sci U S A. 1998;95:15281-6 pubmed..These studies demonstrate that there are multiple activation targets in RNA polymerase II and that RPB5 and the CTD have similar roles in activation. ..
- Choi J, Martin C. The Saccharomyces cerevisiae FAT1 gene encodes an acyl-CoA synthetase that is required for maintenance of very long chain fatty acid levels. J Biol Chem. 1999;274:4671-83 pubmed..Simultaneous disruption of FAA1 and FAA4, which encode long chain (C14-C18) fatty acyl-CoA synthetases, effectively blocks the import of long chain saturated and unsaturated fatty acids. ..
- Philipova D, Mullen J, Maniar H, Lu J, Gu C, Brill S. A hierarchy of SSB protomers in replication protein A. Genes Dev. 1996;10:2222-33 pubmed..coli SSB. The results are consistent with the notion that RPA arose by duplication of an ancestral SSB gene and that tetrameric ssDNA-binding domains and higher order binding are essential features of cellular SSBs. ..
- Ortolan T, Chen L, Tongaonkar P, Madura K. Rad23 stabilizes Rad4 from degradation by the Ub/proteasome pathway. Nucleic Acids Res. 2004;32:6490-500 pubmed
- Kobayashi Y, Zhuang J, PELTZ S, Dougherty J. Identification of a cellular factor that modulates HIV-1 programmed ribosomal frameshifting. J Biol Chem. 2010;285:19776-84 pubmed publisher..This is the first time that a cellular factor has been identified which can promote HIV-1 PRF and highlights HIV-1 PRF as essential for replication and an important but under exploited antiviral drug target. ..
- Singh B, Ansari A, Hampsey M. Detection of gene loops by 3C in yeast. Methods. 2009;48:361-7 pubmed publisher..We anticipate that this method will be generally applicable to detect dynamic, short-range chromatin interactions and will facilitate the characterization of gene loops and their functional consequences. ..
- Mao Y, Sun M, Desai S, Liu L. SUMO-1 conjugation to topoisomerase I: A possible repair response to topoisomerase-mediated DNA damage. Proc Natl Acad Sci U S A. 2000;97:4046-51 pubmed..iii) TOP1 physically interacts with UBC9. (iv) Ubc9 mutant yeast cells expressing human DNA TOP1 was hypersensitive to CPT, suggesting that UBC9/SUMO-1 may be involved in the repair of TOP1-mediated DNA damage. ..
- Belakavadi M, Pandey P, Vijayvargia R, Fondell J. MED1 phosphorylation promotes its association with mediator: implications for nuclear receptor signaling. Mol Cell Biol. 2008;28:3932-42 pubmed publisher..Our findings suggest that ERK phosphorylation of MED1 is a regulatory mechanism that promotes MED1 association with Mediator and, as such, may facilitate a novel feed-forward action of nuclear hormones. ..
- Montano S, Coté M, Fingerman I, Pierce M, Vershon A, Georgiadis M. Crystal structure of the DNA-binding domain from Ndt80, a transcriptional activator required for meiosis in yeast. Proc Natl Acad Sci U S A. 2002;99:14041-6 pubmed
- Togneri J, Cheng Y, Munson M, Hughson F, Carr C. Specific SNARE complex binding mode of the Sec1/Munc-18 protein, Sec1p. Proc Natl Acad Sci U S A. 2006;103:17730-5 pubmed..We propose that vesicle fusion requires a specific interaction between the SM protein and the ternary SNARE complex. ..
- Schauber C, Chen L, Tongaonkar P, Vega I, Lambertson D, Potts W, et al. Rad23 links DNA repair to the ubiquitin/proteasome pathway. Nature. 1998;391:715-8 pubmed..The ubiquitin-like domain of human Rad23 (UbL[HRB]) also interacts with the human proteasome. These results demonstrate that ubiquitin-like domains (UbLs) represent a new class of proteasome-interacting motifs. ..
- Chen B, Hampsey M. Functional interaction between TFIIB and the Rpb2 subunit of RNA polymerase II: implications for the mechanism of transcription initiation. Mol Cell Biol. 2004;24:3983-91 pubmed
- Ortiz P, Kinzy T. Dominant-negative mutant phenotypes and the regulation of translation elongation factor 2 levels in yeast. Nucleic Acids Res. 2005;33:5740-8 pubmed..The observed regulation suggests that the cell needs an optimum amount of active eEF2 to grow properly. This provides information about a new mechanism by which translation is efficiently maintained. ..
- Krishnamurthy S, He X, Reyes Reyes M, Moore C, Hampsey M. Ssu72 Is an RNA polymerase II CTD phosphatase. Mol Cell. 2004;14:387-94 pubmed
- Yue Z, Maldonado E, Pillutla R, Cho H, Reinberg D, Shatkin A. Mammalian capping enzyme complements mutant Saccharomyces cerevisiae lacking mRNA guanylyltransferase and selectively binds the elongating form of RNA polymerase II. Proc Natl Acad Sci U S A. 1997;94:12898-903 pubmed..These results also explain the selective capping of RNA polymerase II transcripts. ..
- Lai C, Boguski M, Broek D, Powers S. Influence of guanine nucleotides on complex formation between Ras and CDC25 proteins. Mol Cell Biol. 1993;13:1345-52 pubmed
- Liu H, Kiledjian M. Scavenger decapping activity facilitates 5' to 3' mRNA decay. Mol Cell Biol. 2005;25:9764-72 pubmed..These data define a new role for the Dcs1p scavenger decapping enzyme and demonstrate a novel mechanism whereby the final step in the 3' mRNA decay pathway can influence 5' to 3' exoribonucleolytic activity. ..
- Mullen J, Chen C, Brill S. Wss1 is a SUMO-dependent isopeptidase that interacts genetically with the Slx5-Slx8 SUMO-targeted ubiquitin ligase. Mol Cell Biol. 2010;30:3737-48 pubmed publisher..The results suggest that Wss1 is a SUMO-dependent isopeptidase that acts on sumoylated substrates as they undergo proteasomal degradation. ..
- Han J, Sabbatini P, White E. Induction of apoptosis by human Nbk/Bik, a BH3-containing protein that interacts with E1B 19K. Mol Cell Biol. 1996;16:5857-64 pubmed..Nbk may therefore represent a novel death regulator which contains only a BH3 that interacts with and antagonizes apoptosis inhibitors such as the E1B 19K protein. ..
- Ii M, Ii T, Brill S. Mus81 functions in the quality control of replication forks at the rDNA and is involved in the maintenance of rDNA repeat number in Saccharomyces cerevisiae. Mutat Res. 2007;625:1-19 pubmed..This is the first evidence that Mus81 functions in quality control of replication forks and that it is involved in the maintenance of rDNA repeats in vivo. ..
- Acton T, Mead J, Steiner A, Vershon A. Scanning mutagenesis of Mcm1: residues required for DNA binding, DNA bending, and transcriptional activation by a MADS-box protein. Mol Cell Biol. 2000;20:1-11 pubmed..This finding is consistent with our earlier results, indicating that DNA bending may have a role in Mcm1 function in the cell. ..
- Xu Z, Norris D. The SFP1 gene product of Saccharomyces cerevisiae regulates G2/M transitions during the mitotic cell cycle and DNA-damage response. Genetics. 1998;150:1419-28 pubmed..In support of this model, overexpression of Sfp1p induces the expression of the PDS1 gene, which is known to encode a protein that regulates the G2 checkpoint. ..
- Kaliraman V, Brill S. Role of SGS1 and SLX4 in maintaining rDNA structure in Saccharomyces cerevisiae. Curr Genet. 2002;41:389-400 pubmed..These results suggest that Sgs1 and Slx4 are not required for bulk DNA synthesis but play redundant roles in maintaining rDNA structure during DNA replication. ..
- Pappas D, Hampsey M. Functional interaction between Ssu72 and the Rpb2 subunit of RNA polymerase II in Saccharomyces cerevisiae. Mol Cell Biol. 2000;20:8343-51 pubmed..These results define Ssu72 as a highly conserved factor that physically and functionally interacts with the RNAP II core machinery during transcription initiation. ..
- Gonzalez C, Ruiz Echevarria M, Vasudevan S, Henry M, Peltz S. The yeast hnRNP-like protein Hrp1/Nab4 marks a transcript for nonsense-mediated mRNA decay. Mol Cell. 2000;5:489-99 pubmed..A mutation in HRP1 that stabilizes nonsense-containing mRNAs abolishes its affinity for the DSE and fails to interact with Upf1p. We present a model describing how Hrp1p marks a transcript for rapid decay. ..
- Kim S, Cabane K, Hampsey M, Reinberg D. Genetic analysis of the YDR1-BUR6 repressor complex reveals an intricate balance among transcriptional regulatory proteins in yeast. Mol Cell Biol. 2000;20:2455-65 pubmed..These results define a delicate balance between positive and negative regulators of transcription operating through the Ydr1-Bur6 repressor complex...
- Kasof G, Goyal L, White E. Btf, a novel death-promoting transcriptional repressor that interacts with Bcl-2-related proteins. Mol Cell Biol. 1999;19:4390-404 pubmed..Thus, btf may represent a novel tumor suppressor gene residing in a unique pathway by which the Bcl-2 family can regulate apoptosis. ..