Genomes and Genes
Experts and Doctors on saccharomyces cerevisiae in Ithaca, New York, United States
Locale: Ithaca, New York, United States
Topic: saccharomyces cerevisiae
Publications160 found, 100 shown here
- Lee S, Alani E. Analysis of interactions between mismatch repair initiation factors and the replication processivity factor PCNA. J Mol Biol. 2006;355:175-84 pubmed..We favor a model in which PCNA acts as a scaffold for consecutive protein-protein interactions that allow for the coordination of MMR steps. ..
- Lee S, Surtees J, Alani E. Saccharomyces cerevisiae MSH2-MSH3 and MSH2-MSH6 complexes display distinct requirements for DNA binding domain I in mismatch recognition. J Mol Biol. 2007;366:53-66 pubmed
- Wanat J, Singh N, Alani E. The effect of genetic background on the function of Saccharomyces cerevisiae mlh1 alleles that correspond to HNPCC missense mutations. Hum Mol Genet. 2007;16:445-52 pubmed..These findings suggest that genetic background can play an important role in determining the pathogenicity of MMR alleles and may explain cases of atypical colorectal cancer inheritance...
- Baird D, Stefan C, Audhya A, Weys S, Emr S. Assembly of the PtdIns 4-kinase Stt4 complex at the plasma membrane requires Ypp1 and Efr3. J Cell Biol. 2008;183:1061-74 pubmed publisher..Efr3 is essential for assembly of both Ypp1 and Stt4 at PIK patches. We conclude that Ypp1 and Efr3 are required for the formation and architecture of Stt4 PIK patches and ultimately PM-based PtdIns4P signaling. ..
- Zanders S, Alani E. The pch2Delta mutation in baker's yeast alters meiotic crossover levels and confers a defect in crossover interference. PLoS Genet. 2009;5:e1000571 pubmed publisher..Based on these and previous observations, we propose a model in which Pch2 functions at an early step in crossover control to ensure that every homolog pair receives an obligate crossover. ..
- Manford A, Xia T, Saxena A, Stefan C, Hu F, Emr S, et al. Crystal structure of the yeast Sac1: implications for its phosphoinositide phosphatase function. EMBO J. 2010;29:1489-98 pubmed publisher..Homology structural modeling of human Fig4/Sac3 allows the mapping of several disease-related mutations and provides a framework for the understanding of the molecular mechanisms of human diseases. ..
- Ling Y, Stefan C, MacGurn J, Audhya A, Emr S. The dual PH domain protein Opy1 functions as a sensor and modulator of PtdIns(4,5)P? synthesis. EMBO J. 2012;31:2882-94 pubmed publisher..Our results also suggest that additional conserved tandem PH domain-containing proteins may play important roles in regulating phosphoinositide signalling. ..
- Vo T, Das J, Meyer M, Cordero N, Akturk N, Wei X, et al. A Proteome-wide Fission Yeast Interactome Reveals Network Evolution Principles from Yeasts to Human. Cell. 2016;164:310-323 pubmed publisher..Overall, FissionNet is a valuable resource for understanding protein functions and their evolution. ..
- García Villegas R, Camacho Villasana Y, Shingú Vázquez M, Cabrera Orefice A, Uribe Carvajal S, Fox T, et al. The Cox1 C-terminal domain is a central regulator of cytochrome c oxidase biogenesis in yeast mitochondria. J Biol Chem. 2017;292:10912-10925 pubmed publisher..Our results indicate that the Cox1 C-terminal end is a key regulator of CcO biogenesis and that it is important for supercomplex formation/stability. ..
- Wilson K, Fortes P, Singh U, Ohno M, Mattaj I, Cerione R. The nuclear cap-binding complex is a novel target of growth factor receptor-coupled signal transduction. J Biol Chem. 1999;274:4166-73 pubmed..Taken together, these data identify the CBC as a nuclear target for growth factor-coupled signal transduction and suggest novel mechanisms by which growth factors can influence gene expression and cell growth. ..
- Liu H, Bretscher A. Purification of tropomyosin from Saccharomyces cerevisiae and identification of related proteins in Schizosaccharomyces and Physarum. Proc Natl Acad Sci U S A. 1989;86:90-3 pubmed..Our detection of tropomyosin in lower eukaryotes suggests that they might have contractile systems very similar to those found in higher organisms...
- Osman M, Cerione R. Iqg1p, a yeast homologue of the mammalian IQGAPs, mediates cdc42p effects on the actin cytoskeleton. J Cell Biol. 1998;142:443-55 pubmed..Mol. Cell. Biol. 15:723-730). We propose that Iqg1p acts as a scaffold to recruit and localize a protein complex involved in actin-based cellular functions and thus mediates the regulatory effects of Cdc42p on the actin cytoskeleton. ..
- Lin J, Vogt V. I-PpoI, the endonuclease encoded by the group I intron PpLSU3, is expressed from an RNA polymerase I transcript. Mol Cell Biol. 1998;18:5809-17 pubmed
- Wagenaar M, Clardy J. Dicerandrols, new antibiotic and cytotoxic dimers produced by the fungus Phomopsis longicolla isolated from an endangered mint. J Nat Prod. 2001;64:1006-9 pubmed..Extensive NMR and HRFABMS experiments were used to identify these new yellow antibiotic and cytotoxic compounds as 2,2'-dimeric tetrahydroxanthones. ..
- Broadley S, Demlow C, Fox T. Peripheral mitochondrial inner membrane protein, Mss2p, required for export of the mitochondrially coded Cox2p C tail in Saccharomyces cerevisiae. Mol Cell Biol. 2001;21:7663-72 pubmed..Taken together, these data indicate that Mss2p plays a role in recognizing the Cox2p C tail in the matrix and promoting its export. ..
- Christensen T, Tye B. Drosophila MCM10 interacts with members of the prereplication complex and is required for proper chromosome condensation. Mol Biol Cell. 2003;14:2206-15 pubmed..The condensation defects observed resemble previously published reports for Orc2, Orc5, and Mcm4 mutants. Our results strengthen and extend the argument that the processes of chromatin condensation and DNA replication are linked. ..
- French J, Cen Y, Vrablik T, Xu P, Allen E, Hanna Rose W, et al. Characterization of nicotinamidases: steady state kinetic parameters, classwide inhibition by nicotinaldehydes, and catalytic mechanism. Biochemistry. 2010;49:10421-39 pubmed publisher..pneumoniae enzyme involving key catalytic residues, a catalytic transition metal ion, and the intermediacy of a thioester intermediate. ..
- Stokol T, Daddona J, Mubayed L, Trimpert J, Kang S. Evaluation of tissue factor expression in canine tumor cells. Am J Vet Res. 2011;72:1097-106 pubmed publisher..Because tumor-associated tissue factor can promote tumor growth and metastasis in human patients, high tissue factor expression could affect the in vivo biological behavior of these tumors in dogs. ..
- Elliott L, Saracco S, Fox T. Multiple roles of the Cox20 chaperone in assembly of Saccharomyces cerevisiae cytochrome c oxidase. Genetics. 2012;190:559-67 pubmed publisher..Under these conditions some mature Cox2 is assembled into cytochrome c oxidase allowing weak respiratory growth. Thus, the Cox20 chaperone has important roles in leader peptide processing, C-tail export, and stabilization of Cox2. ..
- Gayomba S, Jung H, Yan J, Danku J, Rutzke M, Bernal M, et al. The CTR/COPT-dependent copper uptake and SPL7-dependent copper deficiency responses are required for basal cadmium tolerance in A. thaliana. Metallomics. 2013;5:1262-75 pubmed publisher..Together these data show that exposure to excess Cd triggers SPL7-dependent Cu deficiency responses that include Cu uptake and reallocation that are required for basal Cd tolerance in A. thaliana. ..
- Hwang E, Kusch J, Barral Y, Huffaker T. Spindle orientation in Saccharomyces cerevisiae depends on the transport of microtubule ends along polarized actin cables. J Cell Biol. 2003;161:483-8 pubmed..We also present data suggesting that a similar process plays a role in orienting cytoplasmic microtubules in mating yeast cells. ..
- Gaspar M, Aregullin M, Jesch S, Henry S. Inositol induces a profound alteration in the pattern and rate of synthesis and turnover of membrane lipids in Saccharomyces cerevisiae. J Biol Chem. 2006;281:22773-85 pubmed..These results are discussed in relationship to transcriptional regulation known to be linked to lipid metabolism in yeast...
- Su X, Lin Z, Chen W, Jiang H, Zhang S, Lin H. Chemogenomic approach identified yeast YLR143W as diphthamide synthetase. Proc Natl Acad Sci U S A. 2012;109:19983-7 pubmed publisher..Diphthamide synthetase is evolutionarily conserved in eukaryotes. The previously uncharacterized human gene ATPBD4 is the ortholog of yeast YLR143W and fully rescues the deletion of YLR143W in yeast...
- Buchkovich N, Henne W, Tang S, Emr S. Essential N-terminal insertion motif anchors the ESCRT-III filament during MVB vesicle formation. Dev Cell. 2013;27:201-214 pubmed publisher..Our results provide insights into the spatially unique, ESCRT-III-mediated membrane remodeling. ..
- Wang P, Chabes A, Casagrande R, Tian X, Thelander L, Huffaker T. Rnr4p, a novel ribonucleotide reductase small-subunit protein. Mol Cell Biol. 1997;17:6114-21 pubmed..These results suggest that Rnr4p is catalytically inactive but, nonetheless, does play a role in the ribonucleotide reductase complex. ..
- Lei M, Kawasaki Y, Young M, Kihara M, Sugino A, Tye B. Mcm2 is a target of regulation by Cdc7-Dbf4 during the initiation of DNA synthesis. Genes Dev. 1997;11:3365-74 pubmed..Taken together, our data suggest that phosphorylation of Mcm2 and probably other members of the Mcm2-7 proteins by Cdc7-Dbf4 at the G1-to-S phase transition is a critical step in the initiation of DNA synthesis at replication origins. ..
- Studamire B, Price G, Sugawara N, Haber J, Alani E. Separation-of-function mutations in Saccharomyces cerevisiae MSH2 that confer mismatch repair defects but do not affect nonhomologous-tail removal during recombination. Mol Cell Biol. 1999;19:7558-67 pubmed
- Jesch S, Zhao X, Wells M, Henry S. Genome-wide analysis reveals inositol, not choline, as the major effector of Ino2p-Ino4p and unfolded protein response target gene expression in yeast. J Biol Chem. 2005;280:9106-18 pubmed..These results suggest that inositol is the major effector of target gene expression, whereas choline plays a minor role. ..
- Jain R, Vanamee E, Dzikovski B, Buku A, Johnson R, Prakash L, et al. An iron-sulfur cluster in the polymerase domain of yeast DNA polymerase ?. J Mol Biol. 2014;426:301-8 pubmed publisher..Collectively, our work suggests that Pol? is perhaps more sensitive than other DNA polymerases to changes in oxidative stress in eukaryotic cells...
- Haffter P, McMullin T, Fox T. A genetic link between an mRNA-specific translational activator and the translation system in yeast mitochondria. Genetics. 1990;125:495-503 pubmed..Thus our data strongly suggest that the PET123 protein is a component of the mitochondrial translation apparatus that interacts directly with the coxIII-mRNA-specific translational activator PET122. ..
- Chen Y, Tye B. The yeast Mcm1 protein is regulated posttranscriptionally by the flux of glycolysis. Mol Cell Biol. 1995;15:4631-9 pubmed
- Mulero J, Fox T. Alteration of the Saccharomyces cerevisiae COX2 mRNA 5'-untranslated leader by mitochondrial gene replacement and functional interaction with the translational activator protein PET111. Mol Biol Cell. 1993;4:1327-35 pubmed..These results demonstrate an intimate functional interaction between the translational activator protein and the mRNA 5'-UTL and suggest that they may interact directly. ..
- Guzman E, Lis J. Transcription factor TFIIH is required for promoter melting in vivo. Mol Cell Biol. 1999;19:5652-8 pubmed
- Demlow C, Fox T. Activity of mitochondrially synthesized reporter proteins is lower than that of imported proteins and is increased by lowering cAMP in glucose-grown Saccharomyces cerevisiae cells. Genetics. 2003;165:961-74 pubmed..Thus, mitochondrially synthesized proteins may not have the same access to matrix chaperones as cytoplasmically synthesized proteins emerging from the import apparatus. ..
- Pruyne D, Gao L, Bi E, Bretscher A. Stable and dynamic axes of polarity use distinct formin isoforms in budding yeast. Mol Biol Cell. 2004;15:4971-89 pubmed..By coupling different formin isoforms to unique cortical landmarks, yeast uses common cytoskeletal elements to maintain stable and dynamic axes in the same cell. ..
- Yang H, Yang S, Li Y, Hua J. The Arabidopsis BAP1 and BAP2 genes are general inhibitors of programmed cell death. Plant Physiol. 2007;145:135-46 pubmed..The dual roles of BAP and BON genes in repressing defense responses mediated by disease resistance genes and in inhibiting general PCD has implications in understanding the evolution of plant innate immunity. ..
- Henry S, Kohlwein S, Carman G. Metabolism and regulation of glycerolipids in the yeast Saccharomyces cerevisiae. Genetics. 2012;190:317-49 pubmed publisher..Genome-wide genetic analysis indicates coordinate regulation between glycerolipid metabolism and a broad spectrum of metabolic pathways. ..
- Das J, Yu H. HINT: High-quality protein interactomes and their applications in understanding human disease. BMC Syst Biol. 2012;6:92 pubmed publisher..These datasets can be utilized to generate specific hypotheses about specific proteins and/or pathways, as well as analyzing global properties of cellular networks. HINT will be regularly updated and all versions will be tracked. ..
- Paczkowski J, Richardson B, Strassner A, Fromme J. The exomer cargo adaptor structure reveals a novel GTPase-binding domain. EMBO J. 2012;31:4191-203 pubmed publisher..In contrast to appendage domains, which bind accessory factors, we show that the primary role of the FBE domain is to bind Arf1 for recruitment of exomer to membranes. ..
- Donovan K, Bretscher A. Myosin-V is activated by binding secretory cargo and released in coordination with Rab/exocyst function. Dev Cell. 2012;23:769-81 pubmed publisher..All components of this transport cycle are conserved in vertebrates, so these results should be generally applicable to other myosin-V delivery cycles. ..
- Al Sweel N, Raghavan V, Dutta A, Ajith V, Di Vietro L, Khondakar N, et al. mlh3 mutations in baker's yeast alter meiotic recombination outcomes by increasing noncrossover events genome-wide. PLoS Genet. 2017;13:e1006974 pubmed publisher..They also illustrate how defective meiotic components can alter the fate of meiotic recombination intermediates, providing new insights for how meiotic recombination pathways are regulated. ..
- Giardina C, Lis J. Dynamic protein-DNA architecture of a yeast heat shock promoter. Mol Cell Biol. 1995;15:2737-44 pubmed
- Zheng Y, Hart M, Shinjo K, Evans T, Bender A, Cerione R. Biochemical comparisons of the Saccharomyces cerevisiae Bem2 and Bem3 proteins. Delineation of a limit Cdc42 GTPase-activating protein domain. J Biol Chem. 1993;268:24629-34 pubmed..quot; The first two appear to be important for binding to Cdc42Hs and for partial GAP activity. ..
- McMullin T, Fox T. COX3 mRNA-specific translational activator proteins are associated with the inner mitochondrial membrane in Saccharomyces cerevisiae. J Biol Chem. 1993;268:11737-41 pubmed..Amino-terminal sequencing of proteins isolated from mitochondria revealed that PET54 is not processed during import to mitochondria while the PET122 precursor is cleaved between the 8th and 9th residues...
- Alani E. The Saccharomyces cerevisiae Msh2 and Msh6 proteins form a complex that specifically binds to duplex oligonucleotides containing mismatched DNA base pairs. Mol Cell Biol. 1996;16:5604-15 pubmed
- Alani E, Lee S, Kane M, Griffith J, Kolodner R. Saccharomyces cerevisiae MSH2, a mispaired base recognition protein, also recognizes Holliday junctions in DNA. J Mol Biol. 1997;265:289-301 pubmed..This observation suggests that MSH2 protein could directly coordinate the interaction between mismatch repair and genetic recombination observed in genetic studies. ..
- Pruyne D, Schott D, Bretscher A. Tropomyosin-containing actin cables direct the Myo2p-dependent polarized delivery of secretory vesicles in budding yeast. J Cell Biol. 1998;143:1931-45 pubmed..Therefore, actin cables respond to polarity cues independently of the overall distribution of cortical patches and are able to directly target the Myo2p-dependent delivery of secretory vesicles and polarization of growth. ..
- Pruyne D, Bretscher A. Polarization of cell growth in yeast. I. Establishment and maintenance of polarity states. J Cell Sci. 2000;113 ( Pt 3):365-75 pubmed..Given that many of these signaling pathways are present in both fungi and animals, they are probably ancient and conserved mechanisms for regulating polarity. ..
- McGonigle S, Beall M, Pearce E. Eukaryotic initiation factor 2 alpha subunit associates with TGF beta receptors and 14-3-3 epsilon and acts as a modulator of the TGF beta response. Biochemistry. 2002;41:579-87 pubmed..The interaction of these two regulatory proteins with each other and with the TGF beta receptors and their relative expression levels are likely to be important in fine-tuning the regulation of TGF beta signal transduction. ..
- Kim H, Delaney T. Over-expression of TGA5, which encodes a bZIP transcription factor that interacts with NIM1/NPR1, confers SAR-independent resistance in Arabidopsis thaliana to Peronospora parasitica. Plant J. 2002;32:151-63 pubmed..Resistance was retained when a TGA5-accumulating line was combined genetically with a nim1-1 mutation or nahG (salicylate hydroxylase) transgene, indicating that resistance in these plants is due to an SA and SAR-independent mechanism. ..
- Dong Y, Pruyne D, Bretscher A. Formin-dependent actin assembly is regulated by distinct modes of Rho signaling in yeast. J Cell Biol. 2003;161:1081-92 pubmed..These results show that formin function is under the control of three distinct, essential Rho signaling pathways. ..
- Williams E, Perez Martinez X, Fox T. MrpL36p, a highly diverged L31 ribosomal protein homolog with additional functional domains in Saccharomyces cerevisiae mitochondria. Genetics. 2004;167:65-75 pubmed..We propose that MrpL36p represents a highly diverged L31 homolog with derived domains functioning in mRNA selection in yeast mitochondria. ..
- Rahl P, Chen C, Collins R. Elp1p, the yeast homolog of the FD disease syndrome protein, negatively regulates exocytosis independently of transcriptional elongation. Mol Cell. 2005;17:841-53 pubmed..Our results raise the possibility that regulation of polarized exocytosis is an evolutionarily conserved function of the entire Elongator complex and that FD results from a dysregulation of neuronal exocytosis. ..
- Wolyniak M, Blake Hodek K, Kosco K, Hwang E, You L, Huffaker T. The regulation of microtubule dynamics in Saccharomyces cerevisiae by three interacting plus-end tracking proteins. Mol Biol Cell. 2006;17:2789-98 pubmed..These results indicate that interactions among +TIPS can play important roles in the regulation of microtubule dynamics. ..
- Nishant K, Plys A, Alani E. A mutation in the putative MLH3 endonuclease domain confers a defect in both mismatch repair and meiosis in Saccharomyces cerevisiae. Genetics. 2008;179:747-55 pubmed publisher..These studies suggest that the putative endonuclease domain of MLH3 plays an important role in facilitating mismatch repair and meiotic crossing over. ..
- Gaspar M, Hofbauer H, Kohlwein S, Henry S. Coordination of storage lipid synthesis and membrane biogenesis: evidence for cross-talk between triacylglycerol metabolism and phosphatidylinositol synthesis. J Biol Chem. 2011;286:1696-708 pubmed publisher..Thus, we conclude that storage lipid synthesis actively influences membrane phospholipid metabolism in logarithmically growing cells. ..
- Liu W, Santiago Tirado F, Bretscher A. Yeast formin Bni1p has multiple localization regions that function in polarized growth and spindle orientation. Mol Biol Cell. 2012;23:412-22 pubmed publisher..These results define an unexpected complexity in the mechanism of formin localization and function. ..
- Ha K, Clarke B, Brown W. Regulation of the Golgi complex by phospholipid remodeling enzymes. Biochim Biophys Acta. 2012;1821:1078-88 pubmed publisher..This article is part of a Special Issue entitled Lipids and Vesicular Transport. ..
- Chen C, Jomaa A, Ortega J, ALANI E. Pch2 is a hexameric ring ATPase that remodels the chromosome axis protein Hop1. Proc Natl Acad Sci U S A. 2014;111:E44-53 pubmed publisher..Based on these and previous cell biological observations, we suggest that Pch2 impacts meiotic chromosome function by directly regulating Hop1 localization...
- Omnus D, Manford A, Bader J, Emr S, Stefan C. Phosphoinositide kinase signaling controls ER-PM cross-talk. Mol Biol Cell. 2016;27:1170-80 pubmed publisher..Thus PI kinase-mediated ER-PM cross-talk comprises a regulatory system that ensures cellular integrity under membrane stress conditions. ..
- Fitch M, Donato J, Tye B. Mcm7, a subunit of the presumptive MCM helicase, modulates its own expression in conjunction with Mcm1. J Biol Chem. 2003;278:25408-16 pubmed..The multiple roles of Mcm7 in replication initiation, replication elongation, and autoregulation parallel those of the oncoprotein, the large T-antigen of the SV40 virus. ..
- Zheng Y, Bender A, Cerione R. Interactions among proteins involved in bud-site selection and bud-site assembly in Saccharomyces cerevisiae. J Biol Chem. 1995;270:626-30 pubmed..These results also suggest that rather than directly controlling the GEF activity of Cdc24, the primary roles of Rsr1 and Bem1 might be to control the positioning of Cdc24 within the cell. ..
- Li X, Schimenti J, Tye B. Aneuploidy and improved growth are coincident but not causal in a yeast cancer model. PLoS Biol. 2009;7:e1000161 pubmed publisher..Genetic analysis shows their improved growth is due to mutations in just a few loci. In sum, the effects of the mcm4 allele in mice are recapitulated in yeast, and the aneuploidy is not required to maintain improved growth...
- Costanzo M, Seaver E, Fox T. The PET54 gene of Saccharomyces cerevisiae: characterization of a nuclear gene encoding a mitochondrial translational activator and subcellular localization of its product. Genetics. 1989;122:297-305 pubmed..These experiments showed that the PET54 protein is specifically associated with mitochondria, suggesting that it is likely to act directly in coxIII translation. ..
- Green Willms N, Butler C, Dunstan H, Fox T. Pet111p, an inner membrane-bound translational activator that limits expression of the Saccharomyces cerevisiae mitochondrial gene COX2. J Biol Chem. 2001;276:6392-7 pubmed..8-fold in a strain bearing extra copies of PET111 on a high-copy plasmid. Thus, Pet111p could play dual roles in both membrane localization and regulation of Cox2p synthesis within mitochondria. ..
- Suh M, Ye P, Zhang M, Hausmann S, Shuman S, Gnatt A, et al. Fcp1 directly recognizes the C-terminal domain (CTD) and interacts with a site on RNA polymerase II distinct from the CTD. Proc Natl Acad Sci U S A. 2005;102:17314-9 pubmed..We speculate that Fcp1 interaction with the non-CTD site may mediate its stimulatory effect on transcription elongation reported previously. ..
- Munkvold K, Martin M, Bronstein P, Collmer A. A survey of the Pseudomonas syringae pv. tomato DC3000 type III secretion system effector repertoire reveals several effectors that are deleterious when expressed in Saccharomyces cerevisiae. Mol Plant Microbe Interact. 2008;21:490-502 pubmed publisher..HopAA1-1 colocalized with porin to yeast mitochondria and was shown to cause cell death in yeast and plants in a domain-dependent manner. These results support the use of yeast for the study of plant-pathogen effector repertoires. ..
- Richardson B, McDonold C, Fromme J. The Sec7 Arf-GEF is recruited to the trans-Golgi network by positive feedback. Dev Cell. 2012;22:799-810 pubmed publisher..Our results lead us to propose a model in which Arf-GEF recruitment is linked to Golgi maturation via Arf1 activation. ..
- Mulero J, Fox T. Reduced but accurate translation from a mutant AUA initiation codon in the mitochondrial COX2 mRNA of Saccharomyces cerevisiae. Mol Gen Genet. 1994;242:383-90 pubmed
- Bagrodia S, Taylor S, Creasy C, Chernoff J, Cerione R. Identification of a mouse p21Cdc42/Rac activated kinase. J Biol Chem. 1995;270:22731-7 pubmed..These results raise the possibility of an additional level of regulation of the PAK family in vivo. ..
- Ueda H, Abbi S, Zheng C, Guan J. Suppression of Pyk2 kinase and cellular activities by FIP200. J Cell Biol. 2000;149:423-30 pubmed..Together, these results suggest that FIP200 functions as an inhibitor of Pyk2 via binding to its kinase domain. ..
- Huang Q, Liu Q, Hao Q. Crystal structures of Fms1 and its complex with spermine reveal substrate specificity. J Mol Biol. 2005;348:951-9 pubmed..Therefore, the products are spermidine and 3-aminopropanal, rather than 3-(aminopropyl) 4-aminobutyraldehyde and 1,3-diaminoprone. ..
- Rogacheva M, Manhart C, Chen C, Guarné A, SURTEES J, Alani E. Mlh1-Mlh3, a meiotic crossover and DNA mismatch repair factor, is a Msh2-Msh3-stimulated endonuclease. J Biol Chem. 2014;289:5664-73 pubmed publisher..These observations support a direct role for an Mlh1-Mlh3 endonuclease activity in resolving recombination intermediates and in DNA mismatch repair. ..
- Haffter P, McMullin T, Fox T. Functional interactions among two yeast mitochondrial ribosomal proteins and an mRNA-specific translational activator. Genetics. 1991;127:319-26 pubmed
- Richardson B, Fromme J. The exomer cargo adaptor features a flexible hinge domain. Structure. 2013;21:486-92 pubmed publisher..We propose that this flexible hinge domain enables exomer to maintain interactions in the context of a dynamic membrane environment. ..
- Clark D, Rowlett R, Coleman J, Klessig D. Complementation of the yeast deletion mutant DeltaNCE103 by members of the beta class of carbonic anhydrases is dependent on carbonic anhydrase activity rather than on antioxidant activity. Biochem J. 2004;379:609-15 pubmed..Consistent with this conclusion, recombinant NCE103 synthesized in Escherichia coli shows CA activity, and Delta NCE103 expressing the tobacco chloroplast CA exhibits the same sensitivity to H2O2 as the wild-type strain. ..
- Han B, Emr S. Phosphoinositide [PI(3,5)P2] lipid-dependent regulation of the general transcriptional regulator Tup1. Genes Dev. 2011;25:984-95 pubmed publisher..Our findings provide important insights toward understanding how the chromatin architecture and epigenetic status of a gene are regulated by cytoplasmic components. ..
- Plys A, Rogacheva M, Greene E, Alani E. The unstructured linker arms of Mlh1-Pms1 are important for interactions with DNA during mismatch repair. J Mol Biol. 2012;422:192-203 pubmed publisher..These observations are consistent with the unstructured linker domains of MLH proteins providing distinct interactions with DNA during MMR. ..
- Richardson B, Halaby S, Gustafson M, Fromme J. The Sec7 N-terminal regulatory domains facilitate membrane-proximal activation of the Arf1 GTPase. elife. 2016;5: pubmed publisher..This enhancing function of the Sec7 N-terminal domains is consistent with the high rate of Arf1-dependent trafficking to the plasma membrane necessary for maximal cell growth. ..
- Huang Z, Andrianov V, Han Y, Howell S. Identification of arabidopsis proteins that interact with the cauliflower mosaic virus (CaMV) movement protein. Plant Mol Biol. 2001;47:663-75 pubmed..At these sites, fluorescence resonance energy transfer (FRET) between fluorophores was observed indicating an interaction in planta between the CaMV MP and MPI7. ..
- Dhawan M, Wise F, Baeumner A. Development of a laser-induced cell lysis system. Anal Bioanal Chem. 2002;374:421-6 pubmed..Miniaturized lasers and miniaturized cell-lysis chambers will create a simple, field-usable cell lysis system and allow the application of laser-induced cell lysis in micro Total Analysis Systems...
- Henne W, Buchkovich N, Zhao Y, Emr S. The endosomal sorting complex ESCRT-II mediates the assembly and architecture of ESCRT-III helices. Cell. 2012;151:356-71 pubmed publisher..We propose that ESCRT-III has distinct architectural stages that are modulated by ESCRT-II to mediate cargo capture and vesicle formation by ordered assembly. ..
- Liu J, Bratkowski M, Liu X, Niu C, Ke A, Wang H. Visualization of distinct substrate-recruitment pathways in the yeast exosome by EM. Nat Struct Mol Biol. 2014;21:95-102 pubmed publisher..Our results provide mechanistic explanations for several RNA processing scenarios by the eukaryotic exosome and indicate substrate-specific modes of degradation by this complex. ..
- Haffter P, Fox T. Suppression of carboxy-terminal truncations of the yeast mitochondrial mRNA-specific translational activator PET122 by mutations in two new genes, MRP17 and PET127. Mol Gen Genet. 1992;235:64-73 pubmed..These findings suggest the possibility that PET127 could be a novel component of the mitochondrial translation system with a role in promoting accuracy of translational initiation. ..
- Shinjo K, Koland J, Hart M, Narasimhan V, Johnson D, Evans T, et al. Molecular cloning of the gene for the human placental GTP-binding protein Gp (G25K): identification of this GTP-binding protein as the human homolog of the yeast cell-division-cycle protein CDC42. Proc Natl Acad Sci U S A. 1990;87:9853-7 pubmed..The human placental gene, which we designate CDC42Hs, complements the cdc42-1 mutation in S. cerevisiae, which suggests that this GTP-binding protein is the human homolog of the yeast protein. ..
- Brown N, Costanzo M, Fox T. Interactions among three proteins that specifically activate translation of the mitochondrial COX3 mRNA in Saccharomyces cerevisiae. Mol Cell Biol. 1994;14:1045-53 pubmed..Taken together with previous work, these data suggest that a complex containing PET54, PET122, and PET494 mediates the interaction of the COX3 mRNA with mitochondrial ribosomes at the surface of the inner membrane. ..