Experts and Doctors on pseudomonas syringae in Ithaca, New York, United States


Locale: Ithaca, New York, United States
Topic: pseudomonas syringae

Top Publications

  1. Thipyapong P, Hunt M, Steffens J. Antisense downregulation of polyphenol oxidase results in enhanced disease susceptibility. Planta. 2004;220:105-17 pubmed
    ..While PPO B inducibility was the same in both compatible and incompatible interactions, PPO D, E and F were induced to higher levels and with different expression patterns in incompatible interactions. ..
  2. Markel E, Butcher B, Myers C, Stodghill P, Cartinhour S, Swingle B. Regulons of three Pseudomonas syringae pv. tomato DC3000 iron starvation sigma factors. Appl Environ Microbiol. 2013;79:725-7 pubmed publisher
    ..In this study, we identified the genes controlled by three iron starvation sigma factors. Their regulons are composed of a small number of genes likely to be involved in iron uptake. ..
  3. Cohn J, Martin G. Pseudomonas syringae pv. tomato type III effectors AvrPto and AvrPtoB promote ethylene-dependent cell death in tomato. Plant J. 2005;44:139-54 pubmed publisher
    ..AvrPto and AvrPtoB therefore appear to promote enhanced disease in tomato leaves, in part, by upregulating genes involved in ethylene production...
  4. Park S, Liu P, Forouhar F, Vlot A, Tong L, Tietjen K, et al. Use of a synthetic salicylic acid analog to investigate the roles of methyl salicylate and its esterases in plant disease resistance. J Biol Chem. 2009;284:7307-17 pubmed publisher
    ..In TMV-infected tobacco, these studies revealed that critical amounts of MeSA are generated, transmitted, and processed between 48 and 72 h post primary infection. ..
  5. Oh C, Pedley K, Martin G. Tomato 14-3-3 protein 7 positively regulates immunity-associated programmed cell death by enhancing protein abundance and signaling ability of MAPKKK {alpha}. Plant Cell. 2010;22:260-72 pubmed publisher
    ..Our results provide new insights into a role for 14-3-3 proteins in regulating immunity-associated PCD pathways in plants. ..
  6. Lin N, Martin G. Pto- and Prf-mediated recognition of AvrPto and AvrPtoB restricts the ability of diverse pseudomonas syringae pathovars to infect tomato. Mol Plant Microbe Interact. 2007;20:806-15 pubmed
  7. Badel J, Shimizu R, Oh H, Collmer A. A Pseudomonas syringae pv. tomato avrE1/hopM1 mutant is severely reduced in growth and lesion formation in tomato. Mol Plant Microbe Interact. 2006;19:99-111 pubmed
    ..These data suggest that AvrE1 acts within plant cells and promotes lesion formation and that the combined action of AvrE1 and HopM1 is particularly important in promoting bacterial growth in planta. ..
  8. Filiatrault M, Stodghill P, Myers C, Bronstein P, Butcher B, Lam H, et al. Genome-wide identification of transcriptional start sites in the plant pathogen Pseudomonas syringae pv. tomato str. DC3000. PLoS ONE. 2011;6:e29335 pubmed publisher
  9. Abramovitch R, Martin G. AvrPtoB: a bacterial type III effector that both elicits and suppresses programmed cell death associated with plant immunity. FEMS Microbiol Lett. 2005;245:1-8 pubmed publisher
    ..In addition, the "trump model" is proposed to explain how resistance proteins successfully elicit immunity-associated PCD in response to effectors that suppress PCD...

More Information


  1. Abramovitch R, Janjusevic R, Stebbins C, Martin G. Type III effector AvrPtoB requires intrinsic E3 ubiquitin ligase activity to suppress plant cell death and immunity. Proc Natl Acad Sci U S A. 2006;103:2851-6 pubmed
    ..Overall, these data reveal a unique bacterial pathogenesis strategy, where AvrPtoB manipulates the host Ub system and requires intrinsic E3 Ub ligase activity to suppress plant immunity. ..
  2. Filiatrault M, Stodghill P, Wilson J, Butcher B, Chen H, Myers C, et al. CrcZ and CrcX regulate carbon source utilization in Pseudomonas syringae pathovar tomato strain DC3000. RNA Biol. 2013;10:245-55 pubmed publisher
    ..The ncRNAs are comprised of three main subfamilies, named CrcZ, CrcX and CrcY. Most importantly the CrcX subfamily appears to be unique to all P. syringae strains sequenced to date. ..
  3. Meng F, Altier C, Martin G. Salmonella colonization activates the plant immune system and benefits from association with plant pathogenic bacteria. Environ Microbiol. 2013;15:2418-30 pubmed publisher
    ..Our results suggest that Salmonella benefits from the immune-suppressing effects of plant pathogenic bacteria, and this growth enhancement may increase the risk of salmonellosis. ..
  4. Markel E, Maciak C, Butcher B, Myers C, Stodghill P, Bao Z, et al. An extracytoplasmic function sigma factor-mediated cell surface signaling system in Pseudomonas syringae pv. tomato DC3000 regulates gene expression in response to heterologous siderophores. J Bacteriol. 2011;193:5775-83 pubmed publisher
    ..syringae pyoverdine cluster. Additionally, we identified siderophores that induce the activity of PSPTO_1203 and used this information to investigate the functional components of the signal transduction cascade...
  5. Menke F, Van Pelt J, Pieterse C, Klessig D. Silencing of the mitogen-activated protein kinase MPK6 compromises disease resistance in Arabidopsis. Plant Cell. 2004;16:897-907 pubmed
  6. Oh C, Martin G. Tomato 14-3-3 protein TFT7 interacts with a MAP kinase kinase to regulate immunity-associated programmed cell death mediated by diverse disease resistance proteins. J Biol Chem. 2011;286:14129-36 pubmed publisher
    ..Because TFT7 interacts with both SlMAPKKK? and SlMKK2 and also forms a homodimer, we propose that TFT7 may coordinately recruit these client proteins for efficient signal transfer, leading to PCD induction. ..
  7. Zhu Y, Yang H, Mang H, Hua J. Induction of BAP1 by a moderate decrease in temperature is mediated by ICE1 in Arabidopsis. Plant Physiol. 2011;155:580-8 pubmed publisher
    ..Thus, responses to a moderate decrease in temperature may utilize components in the cold response as well as a potentiating signaling involving salicylic acid. ..
  8. Swingle B, Bao Z, Markel E, Chambers A, Cartinhour S. Recombineering using RecTE from Pseudomonas syringae. Appl Environ Microbiol. 2010;76:4960-8 pubmed publisher
    ..Additionally, we illustrate the utility of this recombineering system to make targeted gene disruptions in the P. syringae chromosome...
  9. Kang H, Oh C, Sato M, Katagiri F, Glazebrook J, Takahashi H, et al. Endosome-associated CRT1 functions early in resistance gene-mediated defense signaling in Arabidopsis and tobacco. Plant Cell. 2010;22:918-36 pubmed publisher
    ..Confocal microscopy and subcellular fractionation revealed that CRT1 localizes to endosome-like vesicles, suggesting a key process in resistance protein activation/signaling occurs in this subcellular compartment. ..
  10. Lindeberg M, Cunnac S, Collmer A. Pseudomonas syringae type III effector repertoires: last words in endless arguments. Trends Microbiol. 2012;20:199-208 pubmed publisher
  11. L pez Solanilla E, Bronstein P, Schneider A, Collmer A. HopPtoN is a Pseudomonas syringae Hrp (type III secretion system) cysteine protease effector that suppresses pathogen-induced necrosis associated with both compatible and incompatible plant interactions. Mol Microbiol. 2004;54:353-65 pubmed publisher
    ..These observations reveal that HopPtoN is a TTSS effector that can suppress plant cell death events in both compatible and incompatible interactions...
  12. Oh H, Kvitko B, Morello J, Collmer A. Pseudomonas syringae lytic transglycosylases coregulated with the type III secretion system contribute to the translocation of effector proteins into plant cells. J Bacteriol. 2007;189:8277-89 pubmed
    ..The three Hrp-associated lytic transglycosylases in DC3000 appear to have overlapping functions in contributing to T3SS functions during infection. ..
  13. Lindeberg M, Stavrinides J, Chang J, Alfano J, Collmer A, Dangl J, et al. Proposed guidelines for a unified nomenclature and phylogenetic analysis of type III Hop effector proteins in the plant pathogen Pseudomonas syringae. Mol Plant Microbe Interact. 2005;18:275-82 pubmed publisher
    ..Phylogenetic analyses of previously characterized Hops are described, the results of which have been used to guide their integration into the proposed nomenclature...
  14. Munkvold K, Martin M, Bronstein P, Collmer A. A survey of the Pseudomonas syringae pv. tomato DC3000 type III secretion system effector repertoire reveals several effectors that are deleterious when expressed in Saccharomyces cerevisiae. Mol Plant Microbe Interact. 2008;21:490-502 pubmed publisher
    ..HopAA1-1 colocalized with porin to yeast mitochondria and was shown to cause cell death in yeast and plants in a domain-dependent manner. These results support the use of yeast for the study of plant-pathogen effector repertoires. ..
  15. Wulf J, Pascuzzi P, Fahmy A, Martin G, Nicholson L. The solution structure of type III effector protein AvrPto reveals conformational and dynamic features important for plant pathogenesis. Structure. 2004;12:1257-68 pubmed publisher
    ..The AvrPto structure has a low stability that may facilitate chaperone-independent secretion by the TTSS...
  16. Wang Y, Bao Z, Zhu Y, Hua J. Analysis of temperature modulation of plant defense against biotrophic microbes. Mol Plant Microbe Interact. 2009;22:498-506 pubmed publisher
    ..The inhibition of plant defense response by a moderately high temperature may thus be mediated by other defense signaling components or a combination of multiple factors. ..
  17. Moll S, Schneider D, Stodghill P, Myers C, Cartinhour S, Filiatrault M. Construction of an rsmX co-variance model and identification of five rsmX non-coding RNAs in Pseudomonas syringae pv. tomato DC3000. RNA Biol. 2010;7:508-16 pubmed
    ..We also found that these rsmX ncRNA genes share a conserved upstream region suggesting that their expression is dependent upon the global response regulator, GacA. ..
  18. Thaler J, Agrawal A, Halitschke R. Salicylate-mediated interactions between pathogens and herbivores. Ecology. 2010;91:1075-82 pubmed
    ..We conclude with a predictive model for the expression of defense pathways and their consequences. ..
  19. Filiatrault M, Stodghill P, Bronstein P, Moll S, Lindeberg M, Grills G, et al. Transcriptome analysis of Pseudomonas syringae identifies new genes, noncoding RNAs, and antisense activity. J Bacteriol. 2010;192:2359-72 pubmed publisher
    ..Overall, our approach provides an efficient way to survey global transcriptional activity in bacteria and enables rapid discovery of specific areas in the genome that merit further investigation. ..
  20. Adio A, Casteel C, De Vos M, Kim J, Joshi V, Li B, et al. Biosynthesis and defensive function of Nδ-acetylornithine, a jasmonate-induced Arabidopsis metabolite. Plant Cell. 2011;23:3303-18 pubmed publisher
    ..In the case of P. syringae, growth on a nata1 mutant is reduced compared with wild-type Arabidopsis, but growth in vitro is unaffected by N(δ)-acetylornithine addition. ..
  21. Munkvold K, Martin G. Advances in experimental methods for the elucidation of Pseudomonas syringae effector function with a focus on AvrPtoB. Mol Plant Pathol. 2009;10:777-93 pubmed publisher
    ..Taken together, advances in this field illustrate the need for multiple experimental approaches when elucidating the function of a single effector. ..
  22. Lindeberg M, Cunnac S, Collmer A. The evolution of Pseudomonas syringae host specificity and type III effector repertoires. Mol Plant Pathol. 2009;10:767-75 pubmed publisher
    ..Tools are now available to test several hypotheses for the nature and evolution of P. syringae host specificity and nonhost resistance. ..
  23. Liu P, von Dahl C, Klessig D. The extent to which methyl salicylate is required for signaling systemic acquired resistance is dependent on exposure to light after infection. Plant Physiol. 2011;157:2216-26 pubmed publisher
    ..In addition to resolving the conflicting results concerning MeSA and SAR, this study underscores the importance of environmental factors on the plant's response to infection. ..
  24. Moeder W, Yoshioka K, Klessig D. Involvement of the small GTPase Rac in the defense responses of tobacco to pathogens. Mol Plant Microbe Interact. 2005;18:116-24 pubmed
    ..maculicola ES4326 was suppressed. Thus, the effect DN-OsRac1 expression exerts on the resistance signaling pathway appears to vary depending on the identity of the inoculated pathogen. ..
  25. Cunnac S, Lindeberg M, Collmer A. Pseudomonas syringae type III secretion system effectors: repertoires in search of functions. Curr Opin Microbiol. 2009;12:53-60 pubmed publisher
  26. Schechter L, Roberts K, Jamir Y, Alfano J, Collmer A. Pseudomonas syringae type III secretion system targeting signals and novel effectors studied with a Cya translocation reporter. J Bacteriol. 2004;186:543-55 pubmed
    ..Our results indicate that Cya should be a useful reporter for exploring multiple aspects of the Hrp system in P. syringae...
  27. Lin N, Martin G. An avrPto/avrPtoB mutant of Pseudomonas syringae pv. tomato DC3000 does not elicit Pto-mediated resistance and is less virulent on tomato. Mol Plant Microbe Interact. 2005;18:43-51 pubmed
    ..Our results indicate that AvrPto and AvrPtoB have phenotypically redundant avirulence activity on Pto-expressing tomato and additive virulence activities on susceptible tomato plants. ..
  28. Oh H, Collmer A. Basal resistance against bacteria in Nicotiana benthamiana leaves is accompanied by reduced vascular staining and suppressed by multiple Pseudomonas syringae type III secretion system effector proteins. Plant J. 2005;44:348-59 pubmed publisher
    ..In summary, basal resistance locally alters vascular function and the vascular dye uptake assay should be a useful tool for characterizing effectors that suppress basal resistance...
  29. Schechter L, Vencato M, Jordan K, Schneider S, Schneider D, Collmer A. Multiple approaches to a complete inventory of Pseudomonas syringae pv. tomato DC3000 type III secretion system effector proteins. Mol Plant Microbe Interact. 2006;19:1180-92 pubmed
    ..syringae can be identified efficiently by bioinformatic methods; however, a precise inventory of the subset of Hops that are important in pathogenesis awaits more knowledge based on mutant phenotypes and functions within plants. ..
  30. Li Y, Yang S, Yang H, Hua J. The TIR-NB-LRR gene SNC1 is regulated at the transcript level by multiple factors. Mol Plant Microbe Interact. 2007;20:1449-56 pubmed
    ..The regulation of SNC1 possibly exemplifies a universally complex control of R genes to ensure a repression of R activation under nonstress conditions and a robust activation of defense responses once the R gene is induced. ..
  31. Lindeberg M, Myers C, Collmer A, Schneider D. Roadmap to new virulence determinants in Pseudomonas syringae: insights from comparative genomics and genome organization. Mol Plant Microbe Interact. 2008;21:685-700 pubmed publisher
    ..Distribution of VR among other sequenced bacterial genomes was analyzed and future plans for characterization of this potential reservoir of virulence genes are discussed. ..
  32. Rosebrock T, Zeng L, Brady J, Abramovitch R, Xiao F, Martin G. A bacterial E3 ubiquitin ligase targets a host protein kinase to disrupt plant immunity. Nature. 2007;448:370-4 pubmed
    ..Various wild species of tomato were found to exhibit immunity in response to AvrPtoB(1-387 )and not to full-length AvrPtoB. Thus, by acquiring an E3 ligase domain, AvrPtoB has thwarted a highly conserved host resistance mechanism. ..
  33. Park D, Mirabella R, Bronstein P, Preston G, Haring M, Lim C, et al. Mutations in ?-aminobutyric acid (GABA) transaminase genes in plants or Pseudomonas syringae reduce bacterial virulence. Plant J. 2010;64:318-30 pubmed publisher
    ..GABA may have multiple effects on P. syringae-plant interactions, with elevated levels increasing disease resistance. ..
  34. Swingle B, Thete D, Moll M, Myers C, Schneider D, Cartinhour S. Characterization of the PvdS-regulated promoter motif in Pseudomonas syringae pv. tomato DC3000 reveals regulon members and insights regarding PvdS function in other pseudomonads. Mol Microbiol. 2008;68:871-89 pubmed publisher
    ..We conclude that the role of PvdS as a regulator of pyoverdine synthesis is conserved among the fluorescent pseudomonads, but the promoters recognized by PvdS orthologues may differ subtly from species to species...
  35. Bao Z, Cartinhour S, Swingle B. Substrate and target sequence length influence RecTE(Psy) recombineering efficiency in Pseudomonas syringae. PLoS ONE. 2012;7:e50617 pubmed publisher
    ..These results provide information about the design elements that should be considered when using recombineering...
  36. Butcher B, Bronstein P, Myers C, Stodghill P, Bolton J, Markel E, et al. Characterization of the Fur regulon in Pseudomonas syringae pv. tomato DC3000. J Bacteriol. 2011;193:4598-611 pubmed publisher
  37. Kvitko B, Collmer A. Construction of Pseudomonas syringae pv. tomato DC3000 mutant and polymutant strains. Methods Mol Biol. 2011;712:109-28 pubmed publisher
  38. Ferreira A, Myers C, Gordon J, Martin G, Vencato M, Collmer A, et al. Whole-genome expression profiling defines the HrpL regulon of Pseudomonas syringae pv. tomato DC3000, allows de novo reconstruction of the Hrp cis clement, and identifies novel coregulated genes. Mol Plant Microbe Interact. 2006;19:1167-79 pubmed
    ..syringae genomes, and it supports subsequent identification of effectors and other factors that likely are important to the host-specific virulence of P. syringae. ..
  39. Ramos A, Morello J, Ravindran S, Deng W, Huang H, Collmer A. Identification of Pseudomonas syringae pv. syringae 61 type III secretion system Hrp proteins that can travel the type III pathway and contribute to the translocation of effector proteins into plant cells. J Bacteriol. 2007;189:5773-8 pubmed publisher
    ..T3SS components HrpB, HrpD, HrpF, and HrpP were shown to be pathway substrates and to contribute to elicitation of the plant hypersensitive response and to translocation and secretion of the model effector AvrPto1...
  40. Liu P, Yang Y, Pichersky E, Klessig D. Altering expression of benzoic acid/salicylic acid carboxyl methyltransferase 1 compromises systemic acquired resistance and PAMP-triggered immunity in arabidopsis. Mol Plant Microbe Interact. 2010;23:82-90 pubmed publisher
    ..PAMP-triggered immunity also was compromised in the AtBSMT1 overexpressing plants, although effector-triggered immunity was not. ..
  41. Vlot A, Liu P, Cameron R, Park S, Yang Y, Kumar D, et al. Identification of likely orthologs of tobacco salicylic acid-binding protein 2 and their role in systemic acquired resistance in Arabidopsis thaliana. Plant J. 2008;56:445-56 pubmed publisher
    ..These data suggest that MeSA is a conserved SAR signal in Arabidopsis and tobacco. ..
  42. Worley J, Russell A, Wexler A, Bronstein P, Kvitko B, Krasnoff S, et al. Pseudomonas syringae pv. tomato DC3000 CmaL (PSPTO4723), a DUF1330 family member, is needed to produce L-allo-isoleucine, a precursor for the phytotoxin coronatine. J Bacteriol. 2013;195:287-96 pubmed publisher
    ..This characterization of CmaL identifies a critical missing factor in coronatine production and provides a foundation for further investigation of a member of the widespread DUF1330 protein family...
  43. Oh C, Martin G. Effector-triggered immunity mediated by the Pto kinase. Trends Plant Sci. 2011;16:132-40 pubmed publisher
    ..Here, we review the experimental approaches that have been used in these studies, discuss the proteins that have been identified and characterized, and present a current model of Pto-mediated ETI. ..
  44. Kvitko B, Ramos A, Morello J, Oh H, Collmer A. Identification of harpins in Pseudomonas syringae pv. tomato DC3000, which are functionally similar to HrpK1 in promoting translocation of type III secretion system effectors. J Bacteriol. 2007;189:8059-72 pubmed
    ..syringae T3SS translocon. ..
  45. Zeng L, Velásquez A, Munkvold K, Zhang J, Martin G. A tomato LysM receptor-like kinase promotes immunity and its kinase activity is inhibited by AvrPtoB. Plant J. 2012;69:92-103 pubmed publisher
    ..Finally, we found that Bti9 and Pto interact with AvrPtoB in a structurally similar although not identical fashion, suggesting that Pto may have evolved as a molecular mimic of LysM-RLK kinase domains. ..
  46. Kvitko B, Park D, Vel squez A, Wei C, Russell A, Martin G, et al. Deletions in the repertoire of Pseudomonas syringae pv. tomato DC3000 type III secretion effector genes reveal functional overlap among effectors. PLoS Pathog. 2009;5:e1000388 pubmed publisher
  47. Morello J, Collmer A. Pseudomonas syringae HrpP Is a type III secretion substrate specificity switch domain protein that is translocated into plant cells but functions atypically for a substrate-switching protein. J Bacteriol. 2009;191:3120-31 pubmed publisher
    ..HrpP may function somewhat differently than YscP because the P. syringae T3SS pilus likely varies in length due to differing plant cell walls...