Genomes and Genes
Experts and Doctors on mutation in Ithaca, New York, United States
Locale: Ithaca, New York, United States
Publications295 found, 100 shown here
- Chen R. Phosphorylation and activation of Bub1 on unattached chromosomes facilitate the spindle checkpoint. EMBO J. 2004;23:3113-21 pubmed
- Ilani T, Khanna C, Zhou M, Veenstra T, Bretscher A. Immune synapse formation requires ZAP-70 recruitment by ezrin and CD43 removal by moesin. J Cell Biol. 2007;179:733-46 pubmed..In contrast, moesin dephosphorylation and removal, along with CD43, are necessary to prepare a region of the cell cortex for IS. Thus, ezrin and moesin have distinct and critical functions in the T cell cortex during IS formation. ..
- Holloway J, Booth J, Edelmann W, McGowan C, Cohen P. MUS81 generates a subset of MLH1-MLH3-independent crossovers in mammalian meiosis. PLoS Genet. 2008;4:e1000186 pubmed publisher..This study is the first in-depth analysis of meiotic progression in Mus81-nullizygous mice, and our results implicate the MUS81 pathway as a regulator of crossover frequency and placement in mammals. ..
- Cota C, Liu R, Sumberac T, Jung S, Vencato D, Millet Y, et al. Genetic and phenotypic studies of the dark-like mutant mouse. Genesis. 2008;46:562-73 pubmed publisher..Our results suggest dal and Atrn function in the same pathway and that identification of the dal gene will provide insight into molecular mechanisms of vacuolation in multiple cell types. ..
- Fisher A, DeLisa M. Efficient isolation of soluble intracellular single-chain antibodies using the twin-arginine translocation machinery. J Mol Biol. 2009;385:299-311 pubmed publisher..Our results reveal that the Tat quality control mechanism can be harnessed for molecular evolution of scFvs that are soluble in the reducing cytoplasm of E. coli. ..
- Li Z, Blissard G. The Autographa californica multicapsid nucleopolyhedrovirus GP64 protein: analysis of transmembrane domain length and sequence requirements. J Virol. 2009;83:4447-61 pubmed publisher..Overall, our results show that specific features and amino acid sequences, particularly the length of the hydrophobic TM domain, play critical roles in membrane anchoring, membrane fusion, virus budding, and infectivity...
- Lewis J, Lazarowitz S. Arabidopsis synaptotagmin SYTA regulates endocytosis and virus movement protein cell-to-cell transport. Proc Natl Acad Sci U S A. 2010;107:2491-6 pubmed publisher..Our studies show that SYTA regulates endocytosis, and suggest that distinct virus movement proteins transport their cargos to plasmodesmata for cell-to-cell spread via an endocytic recycling pathway. ..
- Beatty A, Morton D, Kemphues K. The C. elegans homolog of Drosophila Lethal giant larvae functions redundantly with PAR-2 to maintain polarity in the early embryo. Development. 2010;137:3995-4004 pubmed publisher..LGL-1 negatively regulates the accumulation of myosin (NMY-2) on the posterior cortex, representing a possible mechanism by which LGL-1 might contribute to polarity maintenance. ..
- Jaubert M, Bhattacharjee S, Mello A, Perry K, Moffett P. ARGONAUTE2 mediates RNA-silencing antiviral defenses against Potato virus X in Arabidopsis. Plant Physiol. 2011;156:1556-64 pubmed publisher..Furthermore, we find that restriction of PVX on Arabidopsis also depends on AGO2, suggesting that this AGO protein has evolved to specialize in antiviral defenses. ..
- Migone F, Ren Y, Cowan R, Harman R, Nikitin A, Quirk S. Dominant activation of the hedgehog signaling pathway alters development of the female reproductive tract. Genesis. 2012;50:28-40 pubmed publisher..The phenotype observed could be mediated by ectopic expression of Hoxa13 in the uterus and elevated levels of Wnt5a in the oviducts and uterus. ..
- Carter P, Roos J, Kemphues K. Molecular analysis of zyg-11, a maternal-effect gene required for early embryogenesis of Caenorhabditis elegans. Mol Gen Genet. 1990;221:72-80 pubmed..A transcriptional analysis shows that transcription of the gene is not limited to the female germ-line, despite the strict maternal-effect phenotype of zyg-11 mutations. ..
- Gunsalus K, Bonaccorsi S, Williams E, Verni F, Gatti M, Goldberg M. Mutations in twinstar, a Drosophila gene encoding a cofilin/ADF homologue, result in defects in centrosome migration and cytokinesis. J Cell Biol. 1995;131:1243-59 pubmed..We discuss these results in terms of possible roles of the actin-based cytoskeleton in centrosome movement and in cytokinesis. ..
- Schug M, Hutter C, Wetterstrand K, Gaudette M, Mackay T, Aquadro C. The mutation rates of di-, tri- and tetranucleotide repeats in Drosophila melanogaster. Mol Biol Evol. 1998;15:1751-60 pubmed..melanogaster. A positive correlation between repeat unit length and allelic variation suggests that mutation rate increases as the repeat unit lengths of microsatellites increase. ..
- Girvan M, Callaway D, Newman M, Strogatz S. Simple model of epidemics with pathogen mutation. Phys Rev E Stat Nonlin Soft Matter Phys. 2002;65:031915 pubmed..We analyze both models to determine the location of each transition. Our main result is that pathogens in highly connected populations must mutate rapidly in order to remain viable. ..
- Badel J, Charkowski A, Deng W, Collmer A. A gene in the Pseudomonas syringae pv. tomato Hrp pathogenicity island conserved effector locus, hopPtoA1, contributes to efficient formation of bacterial colonies in planta and is duplicated elsewhere in the genome. Mol Plant Microbe Interact. 2002;15:1014-24 pubmed..These results suggest that hopPtoA1 and hopPtoA2 contribute redundantly to the formation of P. syringae pv. tomato DC3000 colonies in Arabidopsis leaves. ..
- Ramakrishnan L, Hess G. On the mechanism of a mutated and abnormally functioning gamma-aminobutyric acid (A) receptor linked to epilepsy. Biochemistry. 2004;43:7534-40 pubmed..We discuss how a knowledge of the mechanism of the mutated receptor indicates an approach for alleviating this dysfunction. ..
- Denby K, Jason L, Murray S, Last R. ups1, an Arabidopsis thaliana camalexin accumulation mutant defective in multiple defence signalling pathways. Plant J. 2005;41:673-84 pubmed..The fact that all three major defence signalling pathways are disrupted in ups1, together with the oxidative stress phenotype, leads us to suggest that UPS1 is involved in ROS signal transduction. ..
- Ku Y, McDonough S, Palaniappan R, Chang C, Chang Y. Novel attenuated Salmonella enterica serovar Choleraesuis strains as live vaccine candidates generated by signature-tagged mutagenesis. Infect Immun. 2005;73:8194-203 pubmed..STM-attenuated live vaccine strains SC4A9 (gifsy-1) and SC2D2 (ssaV) were superior to commercially available live vaccine because they provided both safety and a protective immune response against challenge in pigs. ..
- Isaacson T, Kosma D, Matas A, Buda G, He Y, Yu B, et al. Cutin deficiency in the tomato fruit cuticle consistently affects resistance to microbial infection and biomechanical properties, but not transpirational water loss. Plant J. 2009;60:363-77 pubmed publisher..The three cd mutations were mapped to different loci, and the cloning of CD2 revealed it to encode a homeodomain protein, which we propose acts as a key regulator of cutin biosynthesis in tomato fruit. ..
- Li Z, Blissard G. The pre-transmembrane domain of the Autographa californica multicapsid nucleopolyhedrovirus GP64 protein is critical for membrane fusion and virus infectivity. J Virol. 2009;83:10993-1004 pubmed publisher..These studies suggested that several conserved residues (T463, G460, G462, and G474) were not required for membrane fusion but were important for budding and viral infectivity...
- Huang T, Tohge T, Lytovchenko A, Fernie A, Jander G. Pleiotropic physiological consequences of feedback-insensitive phenylalanine biosynthesis in Arabidopsis thaliana. Plant J. 2010;63:823-35 pubmed publisher..Other observed phenotypes of the adt2-1D mutant include abnormal leaf development, resistance to 5-methyltryptophan, reduced growth of the generalist lepidopteran herbivore Trichoplusia ni (cabbage looper) and increased salt tolerance. ..
- Luo Y, Asai K, Sadaie Y, Helmann J. Transcriptomic and phenotypic characterization of a Bacillus subtilis strain without extracytoplasmic function ? factors. J Bacteriol. 2010;192:5736-45 pubmed publisher..subtilis, confirm that three of these (?(M), ?(W), and ?(X)) play the dominant role in conferring intrinsic resistance to antibiotics, and provide initial insights into the roles of the remaining ECF ? factors. ..
- Anderson V, Ramlall T, Rospigliosi C, Webb W, Eliezer D. Identification of a helical intermediate in trifluoroethanol-induced alpha-synuclein aggregation. Proc Natl Acad Sci U S A. 2010;107:18850-5 pubmed publisher..Given that ?S is known to populate both highly and partially helical states upon association with membranes, these TFE-induced conformations imply relevant pathways for membrane-induced ?S aggregation both in vitro and in vivo...
- Byrnes L, Sondermann H. Structural basis for the nucleotide-dependent dimerization of the large G protein atlastin-1/SPG3A. Proc Natl Acad Sci U S A. 2011;108:2216-21 pubmed publisher..This mechanism is affected in several mutants associated with HSP, providing insights into disease pathogenesis. ..
- Chen J, Scaria J, Chang Y. Phenotypic and transcriptomic response of auxotrophic Mycobacterium avium subsp. paratuberculosis leuD mutant under environmental stress. PLoS ONE. 2012;7:e37884 pubmed publisher..Our results therefore reveal the intricate connection between the metabolism and virulence in MAP...
- Sun H, Tong K, Kasai S, Scott J. Overcoming super-knock down resistance (super-kdr) mediated resistance: multi-halogenated benzyl pyrethroids are more toxic to super-kdr than kdr house flies. Insect Mol Biol. 2016;25:126-37 pubmed publisher..The importance of these results to understanding the frequencies of these resistance alleles in natural populations, the evolution of insecticide resistance and resistance management strategies are discussed. ..
- Shinjo K, Koland J, Hart M, Narasimhan V, Johnson D, Evans T, et al. Molecular cloning of the gene for the human placental GTP-binding protein Gp (G25K): identification of this GTP-binding protein as the human homolog of the yeast cell-division-cycle protein CDC42. Proc Natl Acad Sci U S A. 1990;87:9853-7 pubmed..The human placental gene, which we designate CDC42Hs, complements the cdc42-1 mutation in S. cerevisiae, which suggests that this GTP-binding protein is the human homolog of the yeast protein. ..
- Mulero J, Rosenthal J, Fox T. PET112, a Saccharomyces cerevisiae nuclear gene required to maintain rho+ mitochondrial DNA. Curr Genet. 1994;25:299-304 pubmed..This finding suggests that PET112 has an important general function in mitochondrial gene expression, probably in translation. ..
- Zheng X, Resnick R, Shalloway D. Mitotic activation of protein-tyrosine phosphatase alpha and regulation of its Src-mediated transforming activity by its sites of protein kinase C phosphorylation. J Biol Chem. 2002;277:21922-9 pubmed
- Cao M, Moore C, Helmann J. Bacillus subtilis paraquat resistance is directed by sigmaM, an extracytoplasmic function sigma factor, and is conferred by YqjL and BcrC. J Bacteriol. 2005;187:2948-56 pubmed..Expression of yqjL, but not bcrC, from a xylose-inducible promoter restored PQ resistance to the sigM mutant...
- Nunez L, Jesch S, Gaspar M, Almaguer C, Villa Garcia M, Ruiz Noriega M, et al. Cell wall integrity MAPK pathway is essential for lipid homeostasis. J Biol Chem. 2008;283:34204-17 pubmed publisher..These results indicate that an intact cell wall integrity pathway is required for maintaining proper lipid homeostasis in yeast, especially when cells are grown in the absence of inositol. ..
- Li X, Tye B. Ploidy dictates repair pathway choice under DNA replication stress. Genetics. 2011;187:1031-40 pubmed publisher..In response to replication stress, early events associated with ploidy dictate the repair pathway choice. This study uncovers a fundamental difference between haplophase and diplophase in the maintenance of genome integrity. ..
- Connallon T, Clark A. A general population genetic framework for antagonistic selection that accounts for demography and recurrent mutation. Genetics. 2012;190:1477-89 pubmed publisher..The effects of mutation and demography may generate population differences in overall levels of antagonistic fitness variation, as well as molecular population genetic signatures of balancing selection. ..
- Dean D, Maroja L, Cottrill S, Bomkamp B, Westervelt K, Deitcher D. The wavy Mutation Maps to the Inositol 1,4,5-Trisphosphate 3-Kinase 2 (IP3K2) Gene of Drosophila and Interacts with IP3R to Affect Wing Development. G3 (Bethesda). 2015;6:299-310 pubmed publisher..In proof of concept, a dominant modifier screen revealed that mutations in IP3R strongly suppress the wy phenotype, suggesting that the wy phenotype results from reduced IP4 levels, and/or excessive IP3R signaling. ..
- Wiedmann M, Arvik T, Hurley R, Boor K. General stress transcription factor sigmaB and its role in acid tolerance and virulence of Listeria monocytogenes. J Bacteriol. 1998;180:3650-6 pubmed..Our results suggest that sigmaB-directed genes do not appear to be essential for the spread of L. monocytogenes to mouse liver or spleen at 2 and 4 days following intragastric or intraperitoneal infection. ..
- Xie Y, Counter C, Alani E. Characterization of the repeat-tract instability and mutator phenotypes conferred by a Tn3 insertion in RFC1, the large subunit of the yeast clamp loader. Genetics. 1999;151:499-509 pubmed..From these data we propose that defects in clamp loader function result in DNA replication errors, a subset of which are acted upon by the mismatch-repair system. ..
- Ray K, Wang W, Czarnecki J, Zhang Q, Acland G, Aguirre G. Strategies for identification of mutations causing hereditary retinal diseases in dogs: evaluation of opsin as a candidate gene. J Hered. 1999;90:133-7 pubmed..We conclude that mutations in the rod opsin gene are not associated with PRA or CSNB in the 11 different dog breeds tested. ..
- Roberson M, Meermann S, Morasso M, Mulvaney Musa J, Zhang T. A role for the homeobox protein Distal-less 3 in the activation of the glycoprotein hormone alpha subunit gene in choriocarcinoma cells. J Biol Chem. 2001;276:10016-24 pubmed..Our studies support the conclusion that Dlx 3 is a placental-specific transcriptional regulator that binds to the JRE and contributes to expression of the alpha subunit gene in cells of trophoblast origin. ..
- Han D, Rodriguez L, Guan J. Identification of a novel interaction between integrin beta1 and 14-3-3beta. Oncogene. 2001;20:346-57 pubmed..Taken together, these results identify an interaction between integrin and 14-3-3 proteins and suggest a potentially novel cellular function for 14-3-3 proteins in the regulation of integrin-mediated cell adhesion and signaling events. ..
- Kim H, Delaney T. Over-expression of TGA5, which encodes a bZIP transcription factor that interacts with NIM1/NPR1, confers SAR-independent resistance in Arabidopsis thaliana to Peronospora parasitica. Plant J. 2002;32:151-63 pubmed..Resistance was retained when a TGA5-accumulating line was combined genetically with a nim1-1 mutation or nahG (salicylate hydroxylase) transgene, indicating that resistance in these plants is due to an SA and SAR-independent mechanism. ..
- Kukekova A, Aguirre G, Acland G. Cloning and characterization of canine SHARP1 and its evaluation as a positional candidate for canine early retinal degeneration (erd). Gene. 2003;312:335-43 pubmed
- Lin R, Wang H. Two homologous ATP-binding cassette transporter proteins, AtMDR1 and AtPGP1, regulate Arabidopsis photomorphogenesis and root development by mediating polar auxin transport. Plant Physiol. 2005;138:949-64 pubmed..Together, our results suggest that AtMDR1 and AtPGP1 regulate Arabidopsis (Arabidopsis thaliana) photomorphogenesis and multiple aspects of root development by mediating polar auxin transport...
- Chuang C, Yang D, Bai G, Freeland A, Pruitt S, Schimenti J. Post-transcriptional homeostasis and regulation of MCM2-7 in mammalian cells. Nucleic Acids Res. 2012;40:4914-24 pubmed publisher..Alterations to these pathways cause significant defects in cell growth reflected by disease phenotypes in mice. ..
- Giuliano Garisto Donzelli B, Krasnoff S, Moon Y, Sun Moon Y, Churchill A, Gibson D. Genetic basis of destruxin production in the entomopathogen Metarhizium robertsii. Curr Genet. 2012;58:105-16 pubmed publisher..The DXS mutants exhibited no other detectable changes in morphology and development. ..
- Yamamoto M, Nasrallah J. In planta assessment of the role of thioredoxin h proteins in the regulation of S-locus receptor kinase signaling in transgenic Arabidopsis. Plant Physiol. 2013;163:1387-95 pubmed publisher..Our results are consistent with the conclusion that, contrary to their proposed role, thioredoxin h proteins are not required to prevent the spontaneous activation of SRK in the A. thaliana stigma. ..
- Chakraborty U, George C, Lyndaker A, Alani E. A Delicate Balance Between Repair and Replication Factors Regulates Recombination Between Divergent DNA Sequences in Saccharomyces cerevisiae. Genetics. 2016;202:525-40 pubmed publisher..These observations illustrate the delicate balance that exists between repair and replication factors to optimize genome stability. ..
- Blattner M, Liu D, Robinson B, Huang D, Poliakov A, Gao D, et al. SPOP Mutation Drives Prostate Tumorigenesis In Vivo through Coordinate Regulation of PI3K/mTOR and AR Signaling. Cancer Cell. 2017;31:436-451 pubmed publisher..Using these models and human prostate cancer samples, we show that SPOP mutation activates both PI3K/mTOR and androgen receptor signaling, effectively uncoupling the normal negative feedback between these two pathways. ..
- Studamire B, Price G, Sugawara N, Haber J, Alani E. Separation-of-function mutations in Saccharomyces cerevisiae MSH2 that confer mismatch repair defects but do not affect nonhomologous-tail removal during recombination. Mol Cell Biol. 1999;19:7558-67 pubmed
- Jafri S, Chen S, Calvo J. ilvIH operon expression in Escherichia coli requires Lrp binding to two distinct regions of DNA. J Bacteriol. 2002;184:5293-300 pubmed
- Anderson G, Hanson M. The Arabidopsis Mei2 homologue AML1 binds AtRaptor1B, the plant homologue of a major regulator of eukaryotic cell growth. BMC Plant Biol. 2005;5:2 pubmed..Identification of AML1 as a putative downstream effector of TOR gives valuable insights into the plant-specific mode of action of this critical growth regulator. ..
- Liachko I, Tye B. Mcm10 is required for the maintenance of transcriptional silencing in Saccharomyces cerevisiae. Genetics. 2005;171:503-15 pubmed
- Salzberg L, Helmann J. An antibiotic-inducible cell wall-associated protein that protects Bacillus subtilis from autolysis. J Bacteriol. 2007;189:4671-80 pubmed publisher..subtilis. These results support a model in which induction of YoeB in response to cell envelope stress decreases the activity of autolysins and thereby reduces the rate of antibiotic-dependent cell death...
- Oh H, Kvitko B, Morello J, Collmer A. Pseudomonas syringae lytic transglycosylases coregulated with the type III secretion system contribute to the translocation of effector proteins into plant cells. J Bacteriol. 2007;189:8277-89 pubmed..The three Hrp-associated lytic transglycosylases in DC3000 appear to have overlapping functions in contributing to T3SS functions during infection. ..
- Hatoum A, Roberts J. Prevalence of RNA polymerase stalling at Escherichia coli promoters after open complex formation. Mol Microbiol. 2008;68:17-28 pubmed publisher..In addition to promoter-proximal RNAP trapping, we observed transcription-dependent DNA modifications spanning the tnaA and cspA leader regions up to 100 bp downstream of the transcription start site. ..
- Pungaliya C, Srinivasan J, Fox B, Malik R, Ludewig A, Sternberg P, et al. A shortcut to identifying small molecule signals that regulate behavior and development in Caenorhabditis elegans. Proc Natl Acad Sci U S A. 2009;106:7708-13 pubmed publisher..This study further supports the hypothesis that ascarosides represent a structurally diverse set of nematode signaling molecules regulating major life history traits. ..
- Carmon A, Larson M, Wayne M, MacIntyre R. The rate of unequal crossing over in the dumpy gene from Drosophila melanogaster. J Mol Evol. 2010;70:260-5 pubmed publisher..Nine new repeat length variants were found in these lines allowing us to measure the rate of unequal crossing over in the PF exon. The rate, which for several reasons is an underestimate, is 7.05 x 10(-4) exchanges per generation. ..
- Manford A, Xia T, Saxena A, Stefan C, Hu F, Emr S, et al. Crystal structure of the yeast Sac1: implications for its phosphoinositide phosphatase function. EMBO J. 2010;29:1489-98 pubmed publisher..Homology structural modeling of human Fig4/Sac3 allows the mapping of several disease-related mutations and provides a framework for the understanding of the molecular mechanisms of human diseases. ..
- Ge M, Freed J. Two conserved residues are important for inducing highly ordered membrane domains by the transmembrane domain of influenza hemagglutinin. Biophys J. 2011;100:90-7 pubmed publisher..e., membrane dehydration, is important for vesicle aggregation. The possibility that hydrophobic interaction between the highly ordered membrane domains plays a role in vesicle aggregation and viral fusion is discussed. ..
- Li Z, Rosenbaum M, Venkataraman A, Tam T, Katz E, Angenent L. Bacteria-based AND logic gate: a decision-making and self-powered biosensor. Chem Commun (Camb). 2011;47:3060-2 pubmed publisher..We showed a distinct electrical output signal, despite the complexity and continuous regulation of metabolic reactions of living cells. ..
- Ni Z, Ebata A, Alipanahiramandi E, Lee S. Two SET domain containing genes link epigenetic changes and aging in Caenorhabditis elegans. Aging Cell. 2012;11:315-25 pubmed publisher..We hypothesize that inactivation of SET-26 triggers compensation mechanisms to restore repressive chromatin structure and hence affects chromatin stability to promote longevity. ..
- Bergman Z, Xia X, Amaro I, Huffaker T. Constitutive dynein activity in She1 mutants reveals differences in microtubule attachment at the yeast spindle pole body. Mol Biol Cell. 2012;23:2319-26 pubmed publisher..Overall, these results indicate that inhibition of dynein activity by She1 is important to prevent excessive detachment of cytoplasmic microtubules, particularly in G1 cells. ..
- Unckless R, Howick V, Lazzaro B. Convergent Balancing Selection on an Antimicrobial Peptide in Drosophila. Curr Biol. 2016;26:257-262 pubmed publisher..This pattern of evolution appears to be common in AMPs but is invisible to conventional screens for adaptive evolution that are predicated on elevated rates of amino acid divergence. ..
- Manhart C, Ni X, White M, Ortega J, Surtees J, Alani E. The mismatch repair and meiotic recombination endonuclease Mlh1-Mlh3 is activated by polymer formation and can cleave DNA substrates in trans. PLoS Biol. 2017;15:e2001164 pubmed publisher..Together these observations argue that Mlh1-Mlh3 may not act like a canonical, RuvC-like Holliday junction resolvase and support a novel model in which Mlh1-Mlh3 is loaded onto DNA to form an activated polymer that cleaves DNA. ..
- Williams B, Goldberg M. Determinants of Drosophila zw10 protein localization and function. J Cell Sci. 1994;107 ( Pt 4):785-98 pubmed..These findings indicate that the zw10 protein functions as part of a multicomponent mechanism ensuring proper chromosome segregation at the beginning of anaphase. ..
- Williams B, Garrett Engele C, Li Z, Williams E, Rosenman E, Goldberg M. Two putative acetyltransferases, san and deco, are required for establishing sister chromatid cohesion in Drosophila. Curr Biol. 2003;13:2025-36 pubmed..At least two diverse acetyltransferases play vital roles in regulating sister chromatid cohesion during Drosophila mitosis. ..
- Jarosinski K, Margulis N, Kamil J, Spatz S, Nair V, Osterrieder N. Horizontal transmission of Marek's disease virus requires US2, the UL13 protein kinase, and gC. J Virol. 2007;81:10575-87 pubmed..The experiments clearly showed that US2, UL13, and gC in combination are essential for horizontal transmission of MDV and that none of the genes alone is able to restore this phenotype. ..
- Rairdan G, Collier S, Sacco M, Baldwin T, Boettrich T, Moffett P. The coiled-coil and nucleotide binding domains of the Potato Rx disease resistance protein function in pathogen recognition and signaling. Plant Cell. 2008;20:739-51 pubmed publisher..We describe a model of CC-NB-LRR function wherein the LRR and CC domains coregulate the signaling activity of the NB domain in a recognition-specific manner. ..
- Bollenbach T, Sharwood R, Gutierrez R, Lerbs Mache S, Stern D. The RNA-binding proteins CSP41a and CSP41b may regulate transcription and translation of chloroplast-encoded RNAs in Arabidopsis. Plant Mol Biol. 2009;69:541-52 pubmed publisher..In conclusion, our data suggest that CSP41a and CSP41b stimulate both transcription and translation in the chloroplast. ..
- Pawlowski W, Wang C, Golubovskaya I, Szymaniak J, Shi L, Hamant O, et al. Maize AMEIOTIC1 is essential for multiple early meiotic processes and likely required for the initiation of meiosis. Proc Natl Acad Sci U S A. 2009;106:3603-8 pubmed publisher..The AM1 protein is diffuse in the nucleus during the initiation of meiosis and then binds to chromatin in early meiotic prophase I when it regulates the leptotene-zygotene progression. ..
- Liu Q, Graeff R, Kriksunov I, Jiang H, Zhang B, Oppenheimer N, et al. Structural basis for enzymatic evolution from a dedicated ADP-ribosyl cyclase to a multifunctional NAD hydrolase. J Biol Chem. 2009;284:27637-45 pubmed publisher..The comprehensive structural comparison of CD38 and APDRC presented in this study thus provides insights into the structural determinants for the functional evolution from a cyclase to a hydrolase. ..
- Bolcun Filas E, Bannister L, Barash A, Schimenti K, Hartford S, Eppig J, et al. A-MYB (MYBL1) transcription factor is a master regulator of male meiosis. Development. 2011;138:3319-30 pubmed publisher..The results reveal that MYBL1 is a master regulator of meiotic genes that are involved in multiple processes in spermatocytes, particularly those required for cell cycle progression through pachynema. ..
- Donovan K, Bretscher A. Myosin-V is activated by binding secretory cargo and released in coordination with Rab/exocyst function. Dev Cell. 2012;23:769-81 pubmed publisher..All components of this transport cycle are conserved in vertebrates, so these results should be generally applicable to other myosin-V delivery cycles. ..
- Zhou J, Wang X, Lee J, Lee J. Cell-to-cell movement of two interacting AT-hook factors in Arabidopsis root vascular tissue patterning. Plant Cell. 2013;25:187-201 pubmed publisher..Taken together, this study uncovered a novel mechanism in vascular tissue patterning that requires the intercellular trafficking of two interacting transcription factors. ..
- Licitra B, Millet J, Regan A, Hamilton B, Rinaldi V, Duhamel G, et al. Mutation in spike protein cleavage site and pathogenesis of feline coronavirus. Emerg Infect Dis. 2013;19:1066-73 pubmed publisher..We document a functionally relevant S1/S2 mutation that arises when FIP develops in a cat. These insights into FIP pathogenesis may be useful in development of diagnostic, prevention, and treatment measures against coronaviruses. ..
- Nero D, Bowditch N, Pickert S, MacIntyre R. A genetic and molecular analysis of P-induced mutations at the glucose-6-phosphate dehydrogenase locus in Drosophila melanogaster. Mol Gen Genet. 1989;219:429-38 pubmed..The second mutation was either a simple, non-dysgenically induced point mutation or a very unstable insertion. ..
- Yuan W, Parrish C. Canine parvovirus capsid assembly and differences in mammalian and insect cells. Virology. 2001;279:546-57 pubmed..3, while that recovered from assembled capsids had a pI of about 5.2, similar to that seen for the VP2 of capsids recovered from mammalian cells. ..
- McCune A, Carlson R. Twenty ways to lose your bladder: common natural mutants in zebrafish and widespread convergence of swim bladder loss among teleost fishes. Evol Dev. 2004;6:246-59 pubmed..In this way, developmental bias and selection can work together to produce widespread convergence...
- Thompson J, Doun S, Perry K. Compensatory capsid protein mutations in cucumber mosaic virus confer systemic infectivity in squash (Cucurbita pepo). J Virol. 2006;80:7740-3 pubmed..The possible effect of these compensatory mutations on other, nonsystemically infecting loop mutants was tested with the F117A mutant and found to be neutral, thus indicating a specificity to the observed changes...
- Yang H, Li Y, Hua J. The C2 domain protein BAP1 negatively regulates defense responses in Arabidopsis. Plant J. 2006;48:238-48 pubmed..Furthermore, the BAP1 protein probably functions together with an evolutionarily conserved C2 domain protein BON1/CPN1 to negatively regulate defense responses in plants. ..
- Mu W, Wang W, Schimenti J. An allelic series uncovers novel roles of the BRCT domain-containing protein PTIP in mouse embryonic vascular development. Mol Cell Biol. 2008;28:6439-51 pubmed publisher..These and other data suggest that PTIP has multiple functions, one of which is to promote the formation of transcriptional complexes that provide specificity of developmental gene expression. ..
- Tian C, Sen D, Shi H, Foehr M, Plavskin Y, Vatamaniuk O, et al. The RGM protein DRAG-1 positively regulates a BMP-like signaling pathway in Caenorhabditis elegans. Development. 2010;137:2375-84 pubmed publisher..Our work provides a direct link between RGM proteins and BMP signaling in vivo and a simple and genetically tractable system for mechanistic studies of RGM protein regulation of BMP pathways. ..