Experts and Doctors on electrophoretic mobility shift assay in Ithaca, New York, United States


Locale: Ithaca, New York, United States
Topic: electrophoretic mobility shift assay

Top Publications

  1. Ryan G, Wei Y, Winans S. A LuxR-type repressor of Burkholderia cenocepacia inhibits transcription via antiactivation and is inactivated by its cognate acylhomoserine lactone. Mol Microbiol. 2013;87:94-111 pubmed publisher
    ..CepS is essential for expression of both promoters, regardless of the CepR2 status or OHL concentration. CepS therefore acts downstream of CepR2, and CepR2 appears to function as a CepS antiactivator. ..
  2. Chai Y, Winans S. RepB protein of an Agrobacterium tumefaciens Ti plasmid binds to two adjacent sites between repA and repB for plasmid partitioning and autorepression. Mol Microbiol. 2005;58:1114-29 pubmed publisher
    ..We propose that RepA and RepB form complexes that bind both sites, possibly causing a loop that is important for repression of the repABC operon. Binding at one or both sites may also be required for accurate plasmid partitioning...
  3. Gao J, Scott J. Role of the transcriptional repressor mdGfi-1 in CYP6D1v1-mediated insecticide resistance in the house fly, Musca domestica. Insect Biochem Mol Biol. 2006;36:387-95 pubmed publisher
    ..The significance of these results to understanding insecticide resistance is discussed...
  4. Gabriel S, Helmann J. Contributions of Zur-controlled ribosomal proteins to growth under zinc starvation conditions. J Bacteriol. 2009;191:6116-22 pubmed publisher
    ..These results support a direct and physiologically relevant role for YtiA in mobilizing zinc from ribosomes...
  5. Amin N, Shi H, Liu J. The FoxF/FoxC factor LET-381 directly regulates both cell fate specification and cell differentiation in C. elegans mesoderm development. Development. 2010;137:1451-60 pubmed publisher
    ..Our results unify a diverse set of studies on the functions of FoxF/FoxC factors and provide a model for how FoxF/FoxC factors function during mesoderm development. ..
  6. Nam K, Huang Q, Ke A. Nucleic acid binding surface and dimer interface revealed by CRISPR-associated CasB protein structures. FEBS Lett. 2012;586:3956-61 pubmed publisher
  7. Kim S, Shi H, Lee D, Lis J. Specific SR protein-dependent splicing substrates identified through genomic SELEX. Nucleic Acids Res. 2003;31:1955-61 pubmed
    ..These results indicate that B52 has unique functions in the removal of some introns during development, and plays a critical role in cellular regulatory networks. ..
  8. Kang H, Klessig D. Salicylic acid-inducible Arabidopsis CK2-like activity phosphorylates TGA2. Plant Mol Biol. 2005;57:541-57 pubmed
    ..Possible reasons why mutations in the putative CK2 phosphorylation site had little effect on PR-1 induction by TGA2 are discussed. ..
  9. Chai Y, Tsai C, Cho H, Winans S. Reconstitution of the biochemical activities of the AttJ repressor and the AttK, AttL, and AttM catabolic enzymes of Agrobacterium tumefaciens. J Bacteriol. 2007;189:3674-9 pubmed publisher
    ..Two metabolic intermediates, gamma-hydroxybutyrate and succinic semialdehyde, inactivated the AttJ repressor in vitro and induced attKLM transcription in vivo...

More Information


  1. Schneider A, Abi Abdallah D, Butcher B, Denkers E. Toxoplasma gondii triggers phosphorylation and nuclear translocation of dendritic cell STAT1 while simultaneously blocking IFN?-induced STAT1 transcriptional activity. PLoS ONE. 2013;8:e60215 pubmed publisher
    ..These results reinforce the importance of parasite-mediated blockade of IFN? responses in dendritic cells, while simultaneously showing that T. gondii alone induces STAT1 phosphorylation. ..
  2. Jung C, Hawkins J, Jones S, Xiao Y, Rybarski J, Dillard K, et al. Massively Parallel Biophysical Analysis of CRISPR-Cas Complexes on Next Generation Sequencing Chips. Cell. 2017;170:35-47.e13 pubmed publisher
    ..CHAMP provides a framework for high-throughput, quantitative analysis of protein-DNA interactions on synthetic and genomic DNA. PAPERCLIP. ..