Experts and Doctors on electron spin resonance spectroscopy in Ithaca, New York, United States


Locale: Ithaca, New York, United States
Topic: electron spin resonance spectroscopy

Top Publications

  1. Smith A, Freed J. Dynamics and ordering of lipid spin-labels along the coexistence curve of two membrane phases: an ESR study. Chem Phys Lipids. 2012;165:348-61 pubmed
  2. Heberle F, Wu J, Goh S, Petruzielo R, Feigenson G. Comparison of three ternary lipid bilayer mixtures: FRET and ESR reveal nanodomains. Biophys J. 2010;99:3309-18 pubmed publisher
    ..However, phase domains are much smaller than the optical resolution limit, likely on the order of the Förster distance for energy transfer (R(0), ∼2-8 nm). ..
  3. Costa Filho A, Crepeau R, Borbat P, Ge M, Freed J. Lipid-gramicidin interactions: dynamic structure of the boundary lipid by 2D-ELDOR. Biophys J. 2003;84:3364-78 pubmed
    ..Improved instrumental and simulation methods have been employed. ..
  4. Dzikovski B, Tipikin D, Livshits V, Earle K, Freed J. Multifrequency ESR study of spin-labeled molecules in inclusion compounds with cyclodextrins. Phys Chem Chem Phys. 2009;11:6676-88 pubmed publisher
    ..The visual clarity and the simplicity of analysis of the ESR spectra shown in this work should provide a benchmark for future studies of molecular motion by HFHF ESR. ..
  5. Lai A, Clerico E, Blackburn M, Patel N, Robinson C, Borbat P, et al. Key features of an Hsp70 chaperone allosteric landscape revealed by ion-mobility native mass spectrometry and double electron-electron resonance. J Biol Chem. 2017;292:8773-8785 pubmed publisher
  6. Borbat P, McHaourab H, Freed J. Protein structure determination using long-distance constraints from double-quantum coherence ESR: study of T4 lysozyme. J Am Chem Soc. 2002;124:5304-14 pubmed
    ..This study sets the stage for the use of DQC-ESR for determining the tertiary structure of large proteins with just a small number of long-distance constraints. ..
  7. Eser B, Zhang X, Chanani P, Begley T, Ealick S. From Suicide Enzyme to Catalyst: The Iron-Dependent Sulfide Transfer in Methanococcus jannaschii Thiamin Thiazole Biosynthesis. J Am Chem Soc. 2016;138:3639-42 pubmed publisher
    ..Here, we demonstrate that the Thi4 ortholog from Methanococcus jannaschii uses exogenous sulfide and is catalytic. Structural and biochemical studies on this enzyme elucidate the mechanistic details of the sulfide transfer reactions. ..
  8. Dzikovski B, Borbat P, Freed J. Spin-labeled gramicidin a: channel formation and dissociation. Biophys J. 2004;87:3504-17 pubmed
    ..4-1 mol %) accessible to the ESR methods employed in the study, since this yields non-interacting dimer channels. ..
  9. Sezer D, Freed J, Roux B. Multifrequency electron spin resonance spectra of a spin-labeled protein calculated from molecular dynamics simulations. J Am Chem Soc. 2009;131:2597-605 pubmed publisher
    ..The detailed picture of the spin label emerging from the MD simulations provides useful insight into the molecular origins of the available spectroscopic and crystallographic data. ..

More Information


  1. Ge M, Freed J. Fusion peptide from influenza hemagglutinin increases membrane surface order: an electron-spin resonance study. Biophys J. 2009;96:4925-34 pubmed publisher
    ..Possible roles of bending stresses in promoting membrane fusion are discussed. ..
  2. Borbat P, Freed J. Measuring distances by pulsed dipolar ESR spectroscopy: spin-labeled histidine kinases. Methods Enzymol. 2007;423:52-116 pubmed
  3. Liang Z, Crepeau R, Freed J. Effects of finite pulse width on two-dimensional Fourier transform electron spin resonance. J Magn Reson. 2005;177:247-60 pubmed
    ..Examples are given for different motional regimes, which show how 2D-ELDOR spectra are affected by the finite pulse widths. The theory shows good agreement with 2D-ELDOR experiments performed as a function of pulse width. ..
  4. Georgieva E, Ramlall T, Borbat P, Freed J, Eliezer D. Membrane-bound alpha-synuclein forms an extended helix: long-distance pulsed ESR measurements using vesicles, bicelles, and rodlike micelles. J Am Chem Soc. 2008;130:12856-7 pubmed publisher
  5. Bhatnagar J, Borbat P, Pollard A, Bilwes A, Freed J, Crane B. Structure of the ternary complex formed by a chemotaxis receptor signaling domain, the CheA histidine kinase, and the coupling protein CheW as determined by pulsed dipolar ESR spectroscopy. Biochemistry. 2010;49:3824-41 pubmed publisher
    ..In the context of the hexagonal lattice formed by trimeric transmembrane chemoreceptors, the PDS structure is best accommodated with the P3 domain in the center of a honeycomb edge...
  6. Zhu X, Dzikovski B, Su X, Torelli A, Zhang Y, Ealick S, et al. Mechanistic understanding of Pyrococcus horikoshii Dph2, a [4Fe-4S] enzyme required for diphthamide biosynthesis. Mol Biosyst. 2011;7:74-81 pubmed publisher
    ..Furthermore, our data suggest that the chemistry requires only one [4Fe-4S] cluster to be present in the PhDph2 dimer...
  7. Park S, Borbat P, Gonzalez Bonet G, Bhatnagar J, Pollard A, Freed J, et al. Reconstruction of the chemotaxis receptor-kinase assembly. Nat Struct Mol Biol. 2006;13:400-7 pubmed publisher
    ..Linking layers of ternary complexes with close-packed receptors generates a lattice with reasonable component ratios, cooperative interactions among receptors and accessible sites for modification enzymes...
  8. Georgieva E, Roy A, Grigoryants V, Borbat P, Earle K, Scholes C, et al. Effect of freezing conditions on distances and their distributions derived from Double Electron Electron Resonance (DEER): a study of doubly-spin-labeled T4 lysozyme. J Magn Reson. 2012;216:69-77 pubmed publisher
    ..The assignment of bimodal distributions to tether rotamers as opposed to protein conformations is aided by comparing results using MTSL and 4-Bromo MTSL spin-labels. The latter usually produce narrower distance distributions. ..
  9. Bernskoetter W, Lobkovsky E, Chirik P. Kinetics and mechanism of N2 hydrogenation in bis(cyclopentadienyl) zirconium complexes and dinitrogen functionalization by 1,2-addition of a saturated C-H bond. J Am Chem Soc. 2005;127:14051-61 pubmed
  10. Usery R, Enoki T, Wickramasinghe S, Weiner M, Tsai W, Kim M, et al. Line Tension Controls Liquid-Disordered + Liquid-Ordered Domain Size Transition in Lipid Bilayers. Biophys J. 2017;112:1431-1443 pubmed publisher
    ..We find that other properties of the coexisting Ld and Lo phases do not change significantly in the vicinity of the abrupt domain size transition. ..