Experts and Doctors on saccharomyces cerevisiae proteins in St Louis, Missouri, United States


Locale: St Louis, Missouri, United States
Topic: saccharomyces cerevisiae proteins

Top Publications

  1. Majka J, Niedziela Majka A, Burgers P. The checkpoint clamp activates Mec1 kinase during initiation of the DNA damage checkpoint. Mol Cell. 2006;24:891-901 pubmed
    ..Phosphorylation and binding studies with individual clamp subunits indicate that the Ddc1 subunit mediates the functional interactions with Mec1. ..
  2. Engle E, Fay J. Divergence of the yeast transcription factor FZF1 affects sulfite resistance. PLoS Genet. 2012;8:e1002763 pubmed publisher
    ..Both coding and noncoding changes also affect the expression of many other genes. Our results show how divergence in the coding and promoter region of a transcription factor alters the response to an environmental stress. ..
  3. Klomp L, Lin S, Yuan D, Klausner R, Culotta V, Gitlin J. Identification and functional expression of HAH1, a novel human gene involved in copper homeostasis. J Biol Chem. 1997;272:9221-6 pubmed
    ..These results identify HAH1 as a novel ubiquitously expressed protein, which may play an essential role in antioxidant defense and copper homeostasis in humans. ..
  4. Ghosh A, Steele R, Ray R. Functional domains of c-myc promoter binding protein 1 involved in transcriptional repression and cell growth regulation. Mol Cell Biol. 1999;19:2880-6 pubmed
    ..Taken together, our results indicate that MBP-1 is a complex cellular factor containing multiple transcriptional regulatory domains that play an important role in cell growth regulation. ..
  5. Dover J, Schneider J, Tawiah Boateng M, Wood A, Dean K, Johnston M, et al. Methylation of histone H3 by COMPASS requires ubiquitination of histone H2B by Rad6. J Biol Chem. 2002;277:28368-71 pubmed
    ..Ubiquitination of histone H2B on lysine 123 is the signal for the methylation of histone H3, which leads to silencing of genes located near telomeres. ..
  6. Hardy C. Characterization of an essential Orc2p-associated factor that plays a role in DNA replication. Mol Cell Biol. 1996;16:1832-41 pubmed
    ..These results suggest a role for OAFI in the initiation of DNA replication. Mutant alleles of cdc7 and cdc14 were also isolated in the orc2-1 synthetic lethal screen. Cdc7p, like Oaf1p, also interacts with Orc2p in two-hybrid assays. ..
  7. Flick J, Johnston M. GRR1 of Saccharomyces cerevisiae is required for glucose repression and encodes a protein with leucine-rich repeats. Mol Cell Biol. 1991;11:5101-12 pubmed
  8. Nadolski M, Linder M. Molecular recognition of the palmitoylation substrate Vac8 by its palmitoyltransferase Pfa3. J Biol Chem. 2009;284:17720-30 pubmed publisher
    ..This demonstrates that regions distant from the palmitoylated cysteines are important for recognition by DHHC proteins. ..
  9. Murphy R, Watkins J, Wente S. GLE2, a Saccharomyces cerevisiae homologue of the Schizosaccharomyces pombe export factor RAE1, is required for nuclear pore complex structure and function. Mol Biol Cell. 1996;7:1921-37 pubmed
    ..We propose that Gle2p has a novel role in mediating nuclear transport. ..

More Information


  1. Li X, Burgers P. Cloning and characterization of the essential Saccharomyces cerevisiae RFC4 gene encoding the 37-kDa subunit of replication factor C. J Biol Chem. 1994;269:21880-4 pubmed
    ..However, Rfc4p formed a tight complex with the product of the RFC3 gene which encodes the ATPase of RF-C. ..
  2. Kim H, Huh J, Riles L, Reyes A, Fay J. A noncomplementation screen for quantitative trait alleles in saccharomyces cerevisiae. G3 (Bethesda). 2012;2:753-60 pubmed publisher
    ..Our results highlight the difficulty of identifying small-effect alleles but support the use of noncomplementation as a rapid means of identifying quantitative trait alleles of large effect. ..
  3. Wood A, Schneider J, Dover J, Johnston M, Shilatifard A. The Bur1/Bur2 complex is required for histone H2B monoubiquitination by Rad6/Bre1 and histone methylation by COMPASS. Mol Cell. 2005;20:589-99 pubmed
    ..Our results identify in vivo substrates for Bur1/Bur2, thus linking its role to transcriptional elongation and demonstrating a potential activation mechanism for histone H2B monoubiquitination by the Rad6/Bre1 complex. ..
  4. Mogno I, Vallania F, Mitra R, Cohen B. TATA is a modular component of synthetic promoters. Genome Res. 2010;20:1391-7 pubmed publisher
  5. Bucci M, Wente S. A novel fluorescence-based genetic strategy identifies mutants of Saccharomyces cerevisiae defective for nuclear pore complex assembly. Mol Biol Cell. 1998;9:2439-61 pubmed
    ..Taken together, the identification of such mutants with specific perturbations of NPC structure validates this fluorescence-based strategy as a powerful approach for providing insight into the mechanism of NPC biogenesis. ..
  6. Zhu M, Torres M, Kelley J, Dohlman H, Wang Y. Pheromone- and RSP5-dependent ubiquitination of the G protein beta subunit Ste4 in yeast. J Biol Chem. 2011;286:27147-55 pubmed publisher
  7. Stodola J, Stith C, Burgers P. Proficient Replication of the Yeast Genome by a Viral DNA Polymerase. J Biol Chem. 2016;291:11698-705 pubmed publisher
    ..The chimeric polymerase carries out processive replication with PCNA in vitro; however, in yeast, it requires an increased involvement of the mutagenic translesion DNA polymerase ? during DNA replication. ..
  8. Renn S, Armstrong J, Yang M, Wang Z, An X, Kaiser K, et al. Genetic analysis of the Drosophila ellipsoid body neuropil: organization and development of the central complex. J Neurobiol. 1999;41:189-207 pubmed
    ..The experiments performed here demonstrate the degree of resolution possible from the analysis of enhancer-trap lines and form a useful library of tools for future structure/function studies of the ellipsoid body. ..
  9. Wang Y, Abu Irqeba A, Ayalew M, Suntay K. Sumoylation of transcription factor Tec1 regulates signaling of mitogen-activated protein kinase pathways in yeast. PLoS ONE. 2009;4:e7456 pubmed publisher
    ..Taken together, these findings provide evidence for regulated sumoylation as a mechanism to modulate the activity of Tec1 and validate Ubc9 fusion-directed sumoylation as a useful approach for studying protein sumoylation. ..
  10. Kim H, Fay J. A combined-cross analysis reveals genes with drug-specific and background-dependent effects on drug sensitivity in Saccharomyces cerevisiae. Genetics. 2009;183:1141-51 pubmed publisher
    ..Our results suggest that drug response may often depend on interactions between genes with multi-drug and drug-specific effects. ..
  11. Adames N, Cooper J. Microtubule interactions with the cell cortex causing nuclear movements in Saccharomyces cerevisiae. J Cell Biol. 2000;149:863-74 pubmed
    ..Conversely, microtubule sliding can move the nucleus into the neck even when capture/shrinkage is impaired. ..
  12. Carvalho J, Zheng X. Domains of Gln3p interacting with karyopherins, Ure2p, and the target of rapamycin protein. J Biol Chem. 2003;278:16878-86 pubmed
    ..Finally, we find a lysine/arginine-rich domain essential for the rapamycin-sensitive function, but dispensable for its localization. Our results reveal key domains of Gln3p important for its function and regulation. ..
  13. Lutfiyya L, Johnston M. Two zinc-finger-containing repressors are responsible for glucose repression of SUC2 expression. Mol Cell Biol. 1996;16:4790-7 pubmed
    ..This could explain our observation that MIG2 appears to have little role in glucose repression of other promoters with MIG1-binding sites. ..
  14. Kalastavadi T, True H. Analysis of the [RNQ+] prion reveals stability of amyloid fibers as the key determinant of yeast prion variant propagation. J Biol Chem. 2010;285:20748-55 pubmed publisher
  15. Cannistraro V, Taylor J. Ability of polymerase eta and T7 DNA polymerase to bypass bulge structures. J Biol Chem. 2007;282:11188-96 pubmed
    ..Terminations were not observed, however, when bulge structures approached the site of interaction of the DNA with the extended thumb and thioredoxin. At low temperature, however, terminations did occur at this site. ..
  16. Hardy C, Pautz A. A novel role for Cdc5p in DNA replication. Mol Cell Biol. 1996;16:6775-82 pubmed
    ..cdc5-1 orc2-1 double mutants are synthetically lethal. Levels of Cdc5p were found to be cell cycle regulated and peaked in G2/M. These results suggest a role for Cdc5p and possibly Polo-like kinases at origin complexes. ..
  17. Lin S, Manchester J, Gordon J. Sip2, an N-myristoylated beta subunit of Snf1 kinase, regulates aging in Saccharomyces cerevisiae by affecting cellular histone kinase activity, recombination at rDNA loci, and silencing. J Biol Chem. 2003;278:13390-7 pubmed
  18. Navadgi Patil V, Kumar S, Burgers P. The unstructured C-terminal tail of yeast Dpb11 (human TopBP1) protein is dispensable for DNA replication and the S phase checkpoint but required for the G2/M checkpoint. J Biol Chem. 2011;286:40999-1007 pubmed publisher
    ..Together with aromatic motifs identified previously in the Ddc1 subunit of 9-1-1, another activator of Mec1 kinase, they define a consensus structure for Mec1 activation. ..
  19. Ashrafi K, Lin S, Manchester J, Gordon J. Sip2p and its partner snf1p kinase affect aging in S. cerevisiae. Genes Dev. 2000;14:1872-85 pubmed
    ..Sip2p and Snf1p provide new molecular links between the regulation of cellular energy utilization and aging. ..
  20. Chen C, Chen H, Yeh S, Vittore G, Ho T. Autophagy is enhanced and floral development is impaired in AtHVA22d RNA interference Arabidopsis. Plant Physiol. 2009;149:1679-89 pubmed publisher
    ..Potential mechanisms of this suppression and the roles of abscisic acid-induced HVA22 expression in vegetative and reproductive tissues are discussed. ..
  21. Heil Chapdelaine R, Oberle J, Cooper J. The cortical protein Num1p is essential for dynein-dependent interactions of microtubules with the cortex. J Cell Biol. 2000;151:1337-44 pubmed
    ..These data are consistent with Num1p being an essential element of the cortical attachment mechanism for dynein-dependent sliding of microtubules in the bud. ..
  22. Li Y, Wang Y. Ras protein/cAMP-dependent protein kinase signaling is negatively regulated by a deubiquitinating enzyme, Ubp3, in yeast. J Biol Chem. 2013;288:11358-65 pubmed publisher
    ..Together, our data indicate that Ubp3 is a new regulator of the Ras/PKA signaling pathway and suggest that Ubp3 regulates this pathway by controlling the ubiquitination of Ras GTPase-accelerating protein Ira2. ..
  23. Grishin A, Rothenberg M, Downs M, Blumer K. Mot3, a Zn finger transcription factor that modulates gene expression and attenuates mating pheromone signaling in Saccharomyces cerevisiae. Genetics. 1998;149:879-92 pubmed
    ..We hypothesize that Mot3 regulates expression of factors that attenuate signaling by the pheromone response pathway. ..
  24. Muhua L, Karpova T, Cooper J. A yeast actin-related protein homologous to that in vertebrate dynactin complex is important for spindle orientation and nuclear migration. Cell. 1994;78:669-79 pubmed
    ..The phenotype of a double mutant lacking dynein and Act5p is similar to that of single mutants. Therefore, dynactin complex is in the same pathway as dynein and may be necessary for the action of dynein in vivo. ..
  25. Mitra A, Sept D. Localization of the antimitotic peptide and depsipeptide binding site on beta-tubulin. Biochemistry. 2004;43:13955-62 pubmed
    ..Both the active site and mechanism of microtubule depolymerization predictions are in good agreement with experimental findings. ..
  26. Schmidt S, Gomes X, Burgers P. ATP utilization by yeast replication factor C. III. The ATP-binding domains of Rfc2, Rfc3, and Rfc4 are essential for DNA recognition and clamp loading. J Biol Chem. 2001;276:34784-91 pubmed
  27. Swirnoff A, Apel E, Svaren J, Sevetson B, Zimonjic D, Popescu N, et al. Nab1, a corepressor of NGFI-A (Egr-1), contains an active transcriptional repression domain. Mol Cell Biol. 1998;18:512-24 pubmed
    ..Replacement mutagenesis of residues conserved between these proteins interfered with Nab1 repression, although Nab1 does not function by the same mechanism as Dr1. The human NAB1 genomic locus was mapped to chromosome 2q32.3-33. ..
  28. Wu X, Kim H, Seravalli J, Barycki J, Hart P, Gohara D, et al. Potassium and the K+/H+ Exchanger Kha1p Promote Binding of Copper to ApoFet3p Multi-copper Ferroxidase. J Biol Chem. 2016;291:9796-806 pubmed publisher
    ..Our study reveals a novel functional role of K(+) in the binding of copper to apoFet3p and identifies a K(+)/H(+) exchanger at the secretory pathway as a new molecular factor associated with iron uptake in yeast. ..
  29. Gertz J, Siggia E, Cohen B. Analysis of combinatorial cis-regulation in synthetic and genomic promoters. Nature. 2009;457:215-8 pubmed publisher
    ..Quantitative analyses of synthetic promoter libraries will be an important tool in unravelling the rules underlying combinatorial cis-regulation. ..
  30. Sabina J, Johnston M. Asymmetric signal transduction through paralogs that comprise a genetic switch for sugar sensing in Saccharomyces cerevisiae. J Biol Chem. 2009;284:29635-43 pubmed publisher
  31. Adames N, Oberle J, Cooper J. The surveillance mechanism of the spindle position checkpoint in yeast. J Cell Biol. 2001;153:159-68 pubmed
    ..In both wild-type and mutant cells, mitotic exit was preceded by loss of cytoplasmic microtubules from the neck. Thus, the spindle position checkpoint may monitor such interactions. ..
  32. Galletta B, Chuang D, Cooper J. Distinct roles for Arp2/3 regulators in actin assembly and endocytosis. PLoS Biol. 2008;6:e1 pubmed publisher
    ..Knowledge of these roles provides insight into the in vivo relevance of the dendritic nucleation model for actin assembly. ..
  33. Majka J, Burgers P. Clamping the Mec1/ATR checkpoint kinase into action. Cell Cycle. 2007;6:1157-60 pubmed
    ..Both mechanisms of activation generally upregulate the kinase activity towards all downstream targets. ..
  34. Bylund G, Burgers P. Replication protein A-directed unloading of PCNA by the Ctf18 cohesion establishment complex. Mol Cell Biol. 2005;25:5445-55 pubmed
  35. Iovine M, Wente S. A nuclear export signal in Kap95p is required for both recycling the import factor and interaction with the nucleoporin GLFG repeat regions of Nup116p and Nup100p. J Cell Biol. 1997;137:797-811 pubmed
    ..This finding highlights an important role for a subfamily of GLFG nucleoporins in nuclear export processes. ..
  36. Bertram P, Choi J, Carvalho J, Ai W, Zeng C, Chan T, et al. Tripartite regulation of Gln3p by TOR, Ure2p, and phosphatases. J Biol Chem. 2000;275:35727-33 pubmed
    ..In contrast to the free Gln3p, the Ure2p-bound Gln3p is signifcantly resistant to dephosphorylation. Taken together, these results reveal a tripartite regulatory mechanism by which the phosphorylation of Gln3p is regulated. ..
  37. Wilson T, Grawunder U, Lieber M. Yeast DNA ligase IV mediates non-homologous DNA end joining. Nature. 1997;388:495-8 pubmed
    ..Thus, S. cerevisiae has two distinct and separate ligation pathways. ..
  38. Holly S, Blumer K. PAK-family kinases regulate cell and actin polarization throughout the cell cycle of Saccharomyces cerevisiae. J Cell Biol. 1999;147:845-56 pubmed
    ..Thus, PAK family kinases are primary regulators of cell and actin cytoskeletal polarity throughout most or all of the yeast cell cycle. PAK-family kinases in higher organisms may have similar functions. ..
  39. Ashrafi K, Farazi T, Gordon J. A role for Saccharomyces cerevisiae fatty acid activation protein 4 in regulating protein N-myristoylation during entry into stationary phase. J Biol Chem. 1998;273:25864-74 pubmed
    ..These proteins provide opportunities to further define the molecular mechanisms that regulate survival during stationary phase. ..
  40. Navadgi Patil V, Burgers P. Yeast DNA replication protein Dpb11 activates the Mec1/ATR checkpoint kinase. J Biol Chem. 2008;283:35853-9 pubmed publisher
    ..Our studies suggest that Dpb11 and 9-1-1 may partially compensate for each other during yeast checkpoint function. ..
  41. Erickson J, Johnston M. Genetic and molecular characterization of GAL83: its interaction and similarities with other genes involved in glucose repression in Saccharomyces cerevisiae. Genetics. 1993;135:655-64 pubmed
    ..Such unlinked noncomplementation suggests that Gal83p, Gal82p and Reg1p may interact with one another. Possible roles for GAL83, GAL82 and REG1 are discussed in relation to SNF1, SIP1 and SIP2. ..
  42. Iovine M, Watkins J, Wente S. The GLFG repetitive region of the nucleoporin Nup116p interacts with Kap95p, an essential yeast nuclear import factor. J Cell Biol. 1995;131:1699-713 pubmed
    ..These data show that Nup116p's GLFG region has an essential role in mediating nuclear transport. ..
  43. De Vit M, Waddle J, Johnston M. Regulated nuclear translocation of the Mig1 glucose repressor. Mol Biol Cell. 1997;8:1603-18 pubmed
    ..Our results suggest that a glucose-regulated nuclear import and/or export mechanism controls the activity of Mig1. ..
  44. Muhua L, Adames N, Murphy M, Shields C, Cooper J. A cytokinesis checkpoint requiring the yeast homologue of an APC-binding protein. Nature. 1998;393:487-91 pubmed
    ..Another mutation affects Stt4, a phosphatidylinositol-4-OH kinase. Cold temperature is an environmental stimulus that causes misalignment of the mitotic spindle in yeast and appears to activate this checkpoint mechanism. ..
  45. Cheng L, Hunke L, Hardy C. Cell cycle regulation of the Saccharomyces cerevisiae polo-like kinase cdc5p. Mol Cell Biol. 1998;18:7360-70 pubmed
    ..These results further link the actions of the APC to the completion of mitosis and suggest possible roles for Cdc5p during progression through and completion of mitosis. ..
  46. DeVit M, Johnston M. The nuclear exportin Msn5 is required for nuclear export of the Mig1 glucose repressor of Saccharomyces cerevisiae. Curr Biol. 1999;9:1231-41 pubmed
  47. Ho A, Shen T, Ryan K, Kiseleva E, Levy M, Allen T, et al. Assembly and preferential localization of Nup116p on the cytoplasmic face of the nuclear pore complex by interaction with Nup82p. Mol Cell Biol. 2000;20:5736-48 pubmed
    ..Taken together, these results suggest that Nup82p and Nup116p interact at the cytoplasmic NPC face, with nucleoplasmic Nup116p localization utilizing novel binding partners. ..
  48. Strawn L, Shen T, Wente S. The GLFG regions of Nup116p and Nup100p serve as binding sites for both Kap95p and Mex67p at the nuclear pore complex. J Biol Chem. 2001;276:6445-52 pubmed
    ..Taken together, our in vivo and in vitro results define an essential role for a direct Mex67p-GLFG interaction during mRNA export. ..
  49. White M, Riles L, Cohen B. A systematic screen for transcriptional regulators of the yeast cell cycle. Genetics. 2009;181:435-46 pubmed publisher
  50. Pisat N, Pandey A, MacDiarmid C. MNR2 regulates intracellular magnesium storage in Saccharomyces cerevisiae. Genetics. 2009;183:873-84 pubmed publisher
    ..Together, our results implicate a novel eukaryotic CorA homolog in the regulation of intracellular Mg storage. ..
  51. Tank E, True H. Disease-associated mutant ubiquitin causes proteasomal impairment and enhances the toxicity of protein aggregates. PLoS Genet. 2009;5:e1000382 pubmed publisher
    ..Furthermore, we suggest a model whereby chronic UPS impairment could inflict deleterious consequences on proper protein aggregate sequestration. ..
  52. Johnson D, Knoll L, Rowley N, Gordon J. Genetic analysis of the role of Saccharomyces cerevisiae acyl-CoA synthetase genes in regulating protein N-myristoylation. J Biol Chem. 1994;269:18037-46 pubmed
    ..cerevisiae contains other acyl-CoA synthetases which can activate imported fatty acids. ..