Genomes and Genes
Experts and Doctors on saccharomyces cerevisiae proteins in Detroit, Michigan, United States
Locale: Detroit, Michigan, United States
Topic: saccharomyces cerevisiae proteins
- Ding D, Shi Y, Shaltiel G, Azab A, Pullumbi E, Campbell A, et al. Yeast bioassay for identification of inositol depleting compounds. World J Biol Psychiatry. 2009;10:893-9 pubmed publisher..Inositol depletion in yeast may be exploited as an easy and inexpensive screening test for potential new inositol depleting anti-bipolar drugs. ..
- Ju D, Wang X, Ha S, Fu J, Xie Y. Inhibition of proteasomal degradation of rpn4 impairs nonhomologous end-joining repair of DNA double-strand breaks. PLoS ONE. 2010;5:e9877 pubmed publisher..Inhibition of Rpn4 degradation may result in a concomitant delay of release of Rpn4 and the proteasome from a DSB. This study provides the first evidence for the role of proteasomal degradation of Rpn4 in NHEJ. ..
- Gardocki M, Lopes J. Expression of the yeast PIS1 gene requires multiple regulatory elements including a Rox1p binding site. J Biol Chem. 2003;278:38646-52 pubmed..Furthermore, PI levels were elevated under anaerobic conditions. This is the first evidence that PI levels are affected by regulation of PIS1 transcription. ..
- Li G, Chen S, Thompson M, Greenberg M. New insights into the regulation of cardiolipin biosynthesis in yeast: implications for Barth syndrome. Biochim Biophys Acta. 2007;1771:432-41 pubmed..Further characterization of these regulatory mechanisms holds great potential for the identification of novel functions of CL in mitochondrial and cellular processes. ..
- Mukhopadhyay R, Rosen B. Arsenate reductases in prokaryotes and eukaryotes. Environ Health Perspect. 2002;110 Suppl 5:745-8 pubmed..The yeast Acr2p has an active site motif HC(X)(5)R that is conserved in protein phosphotyrosine phosphatases and rhodanases, suggesting that these three groups of enzymes may have evolved from an ancestral oxyanion-binding protein. ..
- Clifford D, Marinco S, Brush G. The meiosis-specific protein kinase Ime2 directs phosphorylation of replication protein A. J Biol Chem. 2004;279:6163-70 pubmed..Our studies have identified a novel meiosis-specific reaction that targets a key protein required for DNA replication, repair, and recombination. This pathway could be important in differentiating mitotic and meiotic DNA metabolism. ..
- Ju S, Greenberg M. Valproate disrupts regulation of inositol responsive genes and alters regulation of phospholipid biosynthesis. Mol Microbiol. 2003;49:1595-603 pubmed..Thus, in contrast to the effect of chronic VPA treatment, short-term exposure to VPA abrogated the normal response to inositol depletion of inositol responsive genes and led to aberrant synthesis of phospholipids. ..
- Roth A, Papanayotou I, Davis N. The yeast kinase Yck2 has a tripartite palmitoylation signal. Mol Biol Cell. 2011;22:2702-15 pubmed publisher..These soluble proteins have no clear means of accessing membranes and thus may require active capture out of the cytoplasm for palmitoylation by their membrane-localized transferases. ..
- Roth A, Nelson B, Boone C, Davis N. Asg7p-Ste3p inhibition of pheromone signaling: regulation of the zygotic transition to vegetative growth. Mol Cell Biol. 2000;20:8815-25 pubmed
- Sun B, Chen L, Cao W, Roth A, Davis N. The yeast casein kinase Yck3p is palmitoylated, then sorted to the vacuolar membrane with AP-3-dependent recognition of a YXXPhi adaptin sorting signal. Mol Biol Cell. 2004;15:1397-406 pubmed..Although YXXPhi signals have a well-appreciated role in the adaptin-mediated sorting of mammalian cells, this is the first signal of this class to be identified in yeast. ..
- Ju D, Xu H, Wang X, Xie Y. The transcription activation domain of Rpn4 is separate from its degrons. Int J Biochem Cell Biol. 2010;42:282-6 pubmed publisher..This study provides important information for further understanding the biological functions of Rpn4 and the proteasome system. ..
- Kaadige M, Lopes J. Analysis of Opi1p repressor mutants. Curr Genet. 2006;49:30-8 pubmed..A third mutation (L252F) resides in a region of Opi1p with no known function. ..
- Liu M, Brusilow W, Needleman R. Activity of the yeast Tat2p tryptophan permease is sensitive to the anti-tumor agent 4-phenylbutyrate. Curr Genet. 2004;46:256-68 pubmed
- He Q, Greenberg M. Post-translational regulation of phosphatidylglycerolphosphate synthase in response to inositol. Mol Microbiol. 2004;53:1243-9 pubmed..This is the first evidence of phosphorylation of a phospholipid biosynthetic enzyme in response to inositol and identifies a new mechanism of inositol-mediated regulation. ..
- Chen L, Davis N. Recycling of the yeast a-factor receptor. J Cell Biol. 2000;151:731-8 pubmed..A model is presented for how the two Ste3p endocytic modes may collaborate to generate the polarized receptor distribution characteristic of mating cells. ..
- Karunatilaka K, Solem A, Pyle A, Rueda D. Single-molecule analysis of Mss116-mediated group II intron folding. Nature. 2010;467:935-9 pubmed publisher..We propose that Mss116 stimulates group II intron folding through a multi-step process that involves electrostatic stabilization of early intermediates and ATP hydrolysis during the final stages of native state assembly. ..
- Chen M, Hancock L, Lopes J. Transcriptional regulation of yeast phospholipid biosynthetic genes. Biochim Biophys Acta. 2007;1771:310-21 pubmed..Here, we have tried to present a complete summary of the transcription factors and mechanisms that regulate the phospholipid biosynthetic genes. ..
- Zhong Q, Greenberg M. Regulation of phosphatidylglycerophosphate synthase by inositol in Saccharomyces cerevisiae is not at the level of PGS1 mRNA abundance. J Biol Chem. 2003;278:33978-84 pubmed..Thus, whereas inositol controls phosphatidylglycerophosphate synthase activity, regulation of PGS1 expression occurs primarily in response to mitochondrial development cues. ..
- Mukhopadhyay R, Zhou Y, Rosen B. Directed evolution of a yeast arsenate reductase into a protein-tyrosine phosphatase. J Biol Chem. 2003;278:24476-80 pubmed..The ease by which an arsenate reductase can be converted into a protein-tyrosine phosphatase supports this hypothesis. ..
- Medler S, Al Husini N, Raghunayakula S, Mukundan B, Aldea A, Ansari A. Evidence for a complex of transcription factor IIB with poly(A) polymerase and cleavage factor 1 subunits required for gene looping. J Biol Chem. 2011;286:33709-18 pubmed publisher..These results suggest that a complex of TFIIB, CF1 subunits, and Pap1 exists in yeast cells. Furthermore, TFIIB interaction with the CF1 complex and Pap1 is crucial for gene looping and transcriptional regulation. ..
- Zhou J, Zhong Q, Li G, Greenberg M. Loss of cardiolipin leads to longevity defects that are alleviated by alterations in stress response signaling. J Biol Chem. 2009;284:18106-14 pubmed publisher..These findings show for the first time that perturbation of CL synthesis leads to decreased longevity in yeast, which is restored by altering signaling through stress response pathways. ..
- Cook J, Kondapalli K, Rawat S, Childs W, Murugesan Y, Dancis A, et al. Molecular details of the yeast frataxin-Isu1 interaction during mitochondrial Fe-S cluster assembly. Biochemistry. 2010;49:8756-65 pubmed publisher..These results were combined with previously published data to generate a structural model for how the Fe-S cluster protein assembly complex can come together to accomplish Fe-S cluster assembly. ..
- Hancock L, Behta R, Lopes J. Genomic analysis of the Opi- phenotype. Genetics. 2006;173:621-34 pubmed..Regulation in response to inositol is also coordinated with the unfolded protein response (UPR). Consistent with this, several Opi(-) mutants were found to affect the UPR (yhi9, ede1, and vps74). ..
- Ha S, Ju D, Xie Y. The N-terminal domain of Rpn4 serves as a portable ubiquitin-independent degron and is recognized by specific 19S RP subunits. Biochem Biophys Res Commun. 2012;419:226-31 pubmed publisher..This is the first time that specific 19S RP subunits have been identified interacting with a Ub-independent degron. This study provides insight into the mechanism by which Ub-independent substrates are recruited to the 26S proteasome. ..
- Ye C, Lou W, Li Y, Chatzispyrou I, Huttemann M, Lee I, et al. Deletion of the cardiolipin-specific phospholipase Cld1 rescues growth and life span defects in the tafazzin mutant: implications for Barth syndrome. J Biol Chem. 2014;289:3114-25 pubmed publisher..We conclude that transcriptional regulation of Cld1-mediated deacylation of CL influences energy metabolism by modulating the relative contribution of glycolysis and respiration. ..
- Cook J, Bencze K, Jankovic A, Crater A, Busch C, Bradley P, et al. Monomeric yeast frataxin is an iron-binding protein. Biochemistry. 2006;45:7767-77 pubmed..On the basis of our results, we have developed a model for how we believe yeast frataxin interacts with iron. ..
- Rosen B. Families of arsenic transporters. Trends Microbiol. 1999;7:207-12 pubmed..Yeasts extrude arsenite using Acr3p, a plasma membrane carrier protein, or sequester it in vacuoles as the glutathione conjugate using Ycf1p, an ABC transporter...
- Banerjee A, Gregori L, Xu Y, Chau V. The bacterially expressed yeast CDC34 gene product can undergo autoubiquitination to form a multiubiquitin chain-linked protein. J Biol Chem. 1993;268:5668-75 pubmed
- Roth A, Wan J, Bailey A, Sun B, Kuchar J, Green W, et al. Global analysis of protein palmitoylation in yeast. Cell. 2006;125:1003-13 pubmed
- Barnes V, Strunk B, Lee I, Huttemann M, Pile L. Loss of the SIN3 transcriptional corepressor results in aberrant mitochondrial function. BMC Biochem. 2010;11:26 pubmed publisher..The findings that both yeast and Drosophila SIN3 affect mitochondrial activity suggest an evolutionarily conserved role for SIN3 in the control of cellular energy production. ..
- Zhong Q, Gvozdenovic Jeremic J, Webster P, Zhou J, Greenberg M. Loss of function of KRE5 suppresses temperature sensitivity of mutants lacking mitochondrial anionic lipids. Mol Biol Cell. 2005;16:665-75 pubmed..quot; These findings demonstrated that mitochondrial anionic lipids are required for cellular functions that are essential in cell wall biogenesis, the maintenance of cell integrity, and survival at elevated temperature. ..
- Lu L, Roberts G, Simon K, Yu J, Hudson A. Rsf1p, a protein required for respiratory growth of Saccharomyces cerevisiae. Curr Genet. 2003;43:263-72 pubmed..Because of its role in both respiratory growth and mt function, we designate the YMR030 W coding sequence RSF1 (respiration factor 1). ..
- Shen J, Hsu C, Kang B, Rosen B, Bhattacharjee H. The Saccharomyces cerevisiae Arr4p is involved in metal and heat tolerance. Biometals. 2003;16:369-78 pubmed..It is proposed that under heat or metal stress, the soluble ATPase becomes membrane-associated, perhaps through interaction with a partner protein, and that this complex is involved in stress tolerance. ..
- Mukhopadhyay R, Rosen B, Phung L, Silver S. Microbial arsenic: from geocycles to genes and enzymes. FEMS Microbiol Rev. 2002;26:311-25 pubmed..Proposed reaction mechanisms in each case involve three cysteine thiols and S-As bond intermediates, so convergent evolution to similar mechanisms has taken place. ..
- Joshi A, Thompson M, Fei N, Huttemann M, Greenberg M. Cardiolipin and mitochondrial phosphatidylethanolamine have overlapping functions in mitochondrial fusion in Saccharomyces cerevisiae. J Biol Chem. 2012;287:17589-97 pubmed publisher..Taken together, these data demonstrate for the first time in vivo that CL and mitochondrial PE are required to maintain tubular mitochondrial morphology and have overlapping functions in mitochondrial fusion. ..
- Feng Y, Davis N. Akr1p and the type I casein kinases act prior to the ubiquitination step of yeast endocytosis: Akr1p is required for kinase localization to the plasma membrane. Mol Cell Biol. 2000;20:5350-9 pubmed
- Lu L, Roberts G, Oszust C, Hudson A. The YJR127C/ZMS1 gene product is involved in glycerol-based respiratory growth of the yeast Saccharomyces cerevisiae. Curr Genet. 2005;48:235-46 pubmed..Thus, the product of YJR127C/ZMS1 is involved in transcriptional control for genes in both cellular genetic compartments, many of which specify products required for glycerol-based growth, respiration, and other functions. ..
- Mukundan B, Ansari A. Novel role for mediator complex subunit Srb5/Med18 in termination of transcription. J Biol Chem. 2011;286:37053-7 pubmed publisher..These results strongly suggest that Mediator subunit Srb5/Med18 is required for proper termination of transcription of a subset of genes in budding yeast. ..
- Moabbi A, Agarwal N, El Kaderi B, Ansari A. Role for gene looping in intron-mediated enhancement of transcription. Proc Natl Acad Sci U S A. 2012;109:8505-10 pubmed publisher..No effect on splicing, however, was observed in sua7-1 strain. On the basis of these results, we propose a role for gene looping in intron-mediated transcriptional activation of genes in yeast. ..
- Swick L, Kapatos G. A yeast 2-hybrid analysis of human GTP cyclohydrolase I protein interactions. J Neurochem. 2006;97:1447-55 pubmed
- Ju D, Xu H, Wang X, Xie Y. Ubiquitin-mediated degradation of Rpn4 is controlled by a phosphorylation-dependent ubiquitylation signal. Biochim Biophys Acta. 2007;1773:1672-80 pubmed..This study also suggests that binding to E3 may be only a part of the function of a ubiquitylation signal. ..
- Sbrissa D, Ikonomov O, Shisheva A. PIKfyve, a mammalian ortholog of yeast Fab1p lipid kinase, synthesizes 5-phosphoinositides. Effect of insulin. J Biol Chem. 1999;274:21589-97 pubmed..In conclusion, mouse p235 (renamed here PIKfyve) displays a strong in vitro activity for PtdIns 5-P and PtdIns 3,5-P(2) generation, implying PIKfyve has a key role in their biosynthesis. ..
- Roberts G, Hudson A. Rsf1p is required for an efficient metabolic shift from fermentative to glycerol-based respiratory growth in S. cerevisiae. Yeast. 2009;26:95-110 pubmed publisher..These and other data presented here strongly suggest that the transcriptional effects exerted by Rsf1p are mediated via interaction with other transcription factors. ..
- Ju D, Wang L, Mao X, Xie Y. Homeostatic regulation of the proteasome via an Rpn4-dependent feedback circuit. Biochem Biophys Res Commun. 2004;321:51-7 pubmed..This study provides important insights into the mechanism underlying proteasome homeostasis. ..
- Zhong J, Zhang H, Stanyon C, Tromp G, Finley R. A strategy for constructing large protein interaction maps using the yeast two-hybrid system: regulated expression arrays and two-phase mating. Genome Res. 2003;13:2691-9 pubmed..We also present a three-dimensional pooling scheme to further increase the efficiency of large-scale two-hybrid analyses. ..
- Roth A, Feng Y, Chen L, Davis N. The yeast DHHC cysteine-rich domain protein Akr1p is a palmitoyl transferase. J Cell Biol. 2002;159:23-8 pubmed..Finally, our results indicate that palmitoylation within the yeast cell is controlled by multiple PTase specificities. The conserved DHHC-CRD sequence, we propose, is the signature feature of an evolutionarily widespread PTase family. ..
- Clifford D, Stark K, Gardner K, Hoffmann Benning S, Brush G. Mechanistic insight into the Cdc28-related protein kinase Ime2 through analysis of replication protein A phosphorylation. Cell Cycle. 2005;4:1826-33 pubmed..Therefore, the mechanism underlying Ime2 substrate recognition could differ from that of Cdc28. ..
- El Kaderi B, Medler S, Raghunayakula S, Ansari A. Gene looping is conferred by activator-dependent interaction of transcription initiation and termination machineries. J Biol Chem. 2009;284:25015-25 pubmed publisher..Coimmunoprecipitation revealed a physical interaction of Rna15 with TFIIB. We propose that the activators facilitate gene looping through their interaction with TFIIB during transcriptional activation of genes. ..
- Ju D, Xie Y. A synthetic defect in protein degradation caused by loss of Ufd4 and Rad23. Biochem Biophys Res Commun. 2006;341:648-52 pubmed..The current work also provides a direction for further investigation of the physiological functions of the UFD pathway. ..
- Liu Z, Shen J, Carbrey J, Mukhopadhyay R, Agre P, Rosen B. Arsenite transport by mammalian aquaglyceroporins AQP7 and AQP9. Proc Natl Acad Sci U S A. 2002;99:6053-8 pubmed
- Jani N, Lopes J. Transcription regulation of the Saccharomyces cerevisiae PIS1 gene by inositol and the pleiotropic regulator, Ume6p. Mol Microbiol. 2008;70:1529-39 pubmed publisher..Thus, we demonstrate novel regulation of the PIS1 gene by Ume6p. ..
- Robinson K, Koepke J, Kharodawala M, Lopes J. A network of yeast basic helix-loop-helix interactions. Nucleic Acids Res. 2000;28:4460-6 pubmed..Ino2p is the only protein in common with the data presented here. Our finding that Ino4p interacts with five bHLH proteins suggests that Ino4p is likely to be a central player in the coordination of multiple biological processes. ..
- Ye C, Bandara W, Greenberg M. Regulation of inositol metabolism is fine-tuned by inositol pyrophosphates in Saccharomyces cerevisiae. J Biol Chem. 2013;288:24898-908 pubmed publisher..We conclude that INO1 transcription is fine-tuned by the synthesis of inositol pyrophosphates, and we propose a model in which modulation of Kcs1 controls INO1 transcription by regulating synthesis of inositol pyrophosphates...
- Ju D, Wang X, Xu H, Xie Y. The armadillo repeats of the Ufd4 ubiquitin ligase recognize ubiquitin-fusion proteins. FEBS Lett. 2007;581:265-70 pubmed..Disruption of the ARM repeats abolishes the ubiquitylating activity of Ufd4. This study uncovers a new role of the ARM repeats in protein ubiquitylation. ..
- Wang Z, Sheluho D, Gatti D, Ackerman S. The alpha-subunit of the mitochondrial F(1) ATPase interacts directly with the assembly factor Atp12p. EMBO J. 2000;19:1486-93 pubmed..These studies provide the basis for a model of F(1) assembly in which Atp12p is released from the alpha-subunit in exchange for a beta-subunit to form the interface that contains the non-catalytic adenine nucleotide-binding site. ..
- Wang Z, Ackerman S. The assembly factor Atp11p binds to the beta-subunit of the mitochondrial F(1)-ATPase. J Biol Chem. 2000;275:5767-72 pubmed..This observation suggests that the alpha-subunits may exchange for bound Atp11p during the process of F(1) assembly. ..
- Ghosh M, Shen J, Rosen B. Pathways of As(III) detoxification in Saccharomyces cerevisiae. Proc Natl Acad Sci U S A. 1999;96:5001-6 pubmed..Disruption of either the ACR3 or YCF1 gene results in sensitivity to arsenite and disruption of both genes produces additive hypersensitivity. Thus, Acr3p and Ycf1p represent separate pathways for the detoxification of arsenite in yeast...
- Chen S, Liu D, Finley R, Greenberg M. Loss of mitochondrial DNA in the yeast cardiolipin synthase crd1 mutant leads to up-regulation of the protein kinase Swe1p that regulates the G2/M transition. J Biol Chem. 2010;285:10397-407 pubmed publisher..These results suggest that anionic phospholipids are required for processes that are essential for normal cell division in rho degrees cells. ..
- Chen L, Lopes J. Multiple bHLH proteins regulate CIT2 expression in Saccharomyces cerevisiae. Yeast. 2010;27:345-59 pubmed publisher..Collectively, these results support the model that yeast bHLH proteins coordinate different biological pathways. ..
- Patil V, Fox J, Gohil V, Winge D, Greenberg M. Loss of cardiolipin leads to perturbation of mitochondrial and cellular iron homeostasis. J Biol Chem. 2013;288:1696-705 pubmed publisher..Increased expression of ATM1 or YAP1 did not rescue the Fe-S defects in crd1?. These findings show for the first time that CL is required for Fe-S biogenesis to maintain mitochondrial and cellular iron homeostasis. ..
- Sbrissa D, Ikonomov O, Strakova J, Dondapati R, Mlak K, Deeb R, et al. A mammalian ortholog of Saccharomyces cerevisiae Vac14 that associates with and up-regulates PIKfyve phosphoinositide 5-kinase activity. Mol Cell Biol. 2004;24:10437-47 pubmed..These data demonstrate a major role of the PIKfyve-associated hVac14 protein in activating PIKfyve and thereby regulating PtdIns(3,5)P(2) synthesis and endomembrane homeostasis in mammalian cells. ..
- Politis E, Roth A, Davis N. Transmembrane topology of the protein palmitoyl transferase Akr1. J Biol Chem. 2005;280:10156-63 pubmed..Finally, extrapolating from the Akr1p topology, topology models are proposed for other DHHC protein family members. ..
- Yu W, Daniel J, Mehta D, Maddipati K, Greenberg M. MCK1 is a novel regulator of myo-inositol phosphate synthase (MIPS) that is required for inhibition of inositol synthesis by the mood stabilizer valproate. PLoS ONE. 2017;12:e0182534 pubmed publisher..These findings indicate that VPA-induced MIPS inhibition is Mck1-dependent, and suggest a model that unifies two current hypotheses of the mechanism of action of VPA-inositol depletion and GSK3 inhibition. ..
- Ju D, Wang X, Xu H, Xie Y. Genome-wide analysis identifies MYND-domain protein Mub1 as an essential factor for Rpn4 ubiquitylation. Mol Cell Biol. 2008;28:1404-12 pubmed..Together, these data suggest that Mub1 and Ubr2 cooperate to transfer ubiquitin to Rpn4 from Rad6 and that Mub1 may switch from a partner to a substrate of the Ubr2/Rad6 ubiquitin ligase. ..
- Gu Z, Valianpour F, Chen S, Vaz F, Hakkaart G, Wanders R, et al. Aberrant cardiolipin metabolism in the yeast taz1 mutant: a model for Barth syndrome. Mol Microbiol. 2004;51:149-58 pubmed..These results indicate that the taz1Delta mutant is an excellent genetic tool for the study of CL remodelling and may serve as a model system for the study of Barth syndrome. ..
- Bartrand A, Iyasu D, Marinco S, Brush G. Evidence of meiotic crossover control in Saccharomyces cerevisiae through Mec1-mediated phosphorylation of replication protein A. Genetics. 2006;172:27-39 pubmed..These results suggest a new function of Mec1 that operates through RPA to locally control reciprocal recombination. ..
- Ackerman S. Atp11p and Atp12p are chaperones for F(1)-ATPase biogenesis in mitochondria. Biochim Biophys Acta. 2002;1555:101-5 pubmed..Comprehensive knowledge of Atp11p and Atp12p activities in mitochondria bears relevance to human physiology and disease as these chaperone actions are now known to exist in mitochondria of human cells. ..
- Wang Z, Schmid K, Ackerman S. The Drosophila gene 2A5 complements the defect in mitochondrial F1-ATPase assembly in yeast lacking the molecular chaperone Atp11p. FEBS Lett. 1999;452:305-8 pubmed..Furthermore, the product of this Drosophila gene was shown to interact with the S. cerevisiae F1 beta subunit in the yeast two-hybrid assay. These results indicate that Atp11p function is conserved in higher eukaryotes. ..
- Sawarynski K, Najor N, Kepsel A, Brush G. Sic1-induced DNA rereplication during meiosis. Proc Natl Acad Sci U S A. 2009;106:232-7 pubmed publisher..Our results provide strong evidence that CDK activity is required to prevent inappropriate initiation of DNA synthesis before the meiotic divisions. ..
- Chen S, Tarsio M, Kane P, Greenberg M. Cardiolipin mediates cross-talk between mitochondria and the vacuole. Mol Biol Cell. 2008;19:5047-58 pubmed publisher..Our results demonstrate the existence of a novel mitochondria-vacuole signaling pathway mediated by CL synthesis. ..
- Mukhopadhyay R, Shi J, Rosen B. Purification and characterization of ACR2p, the Saccharomyces cerevisiae arsenate reductase. J Biol Chem. 2000;275:21149-57 pubmed..This suggests that during the catalytic cycle, Acr2p forms a mixed disulfide with GSH before being reduced by glutaredoxin to regenerate the active Acr2p reductase. ..
- Azab A, He Q, Ju S, Li G, Greenberg M. Glycogen synthase kinase-3 is required for optimal de novo synthesis of inositol. Mol Microbiol. 2007;63:1248-58 pubmed..These results demonstrate for the first time that GSK-3 is required for optimal myo-inositol-3 phosphate synthase activity and de novo inositol biosynthesis, and that loss of GSK-3 activity causes inositol depletion. ..
- Wang X, Xu H, Ha S, Ju D, Xie Y. Proteasomal degradation of Rpn4 in Saccharomyces cerevisiae is critical for cell viability under stressed conditions. Genetics. 2010;184:335-42 pubmed publisher..Rpn4 thus represents an important stress-responsive mediator whose degradation as well as availability are critical for cell survival under stressed conditions. ..
- Brush G, Najor N, Dombkowski A, Cukovic D, Sawarynski K. Yeast IME2 functions early in meiosis upstream of cell cycle-regulated SBF and MBF targets. PLoS ONE. 2012;7:e31575 pubmed publisher..Thus, Ime2 may functionally resemble Cln3/Cdk1 in promoting S phase entry, or it could play a role even further upstream in the corresponding meiotic cascade. ..
- Kaadige M, Lopes J. Opi1p, Ume6p and Sin3p control expression from the promoter of the INO2 regulatory gene via a novel regulatory cascade. Mol Microbiol. 2003;48:823-32 pubmed..We also show that the UME6 gene does not affect the expression of an OPI1-cat reporter. This suggests that Ume6p does not regulate INO2 expression indirectly by regulating OPI1 expression. ..
- Ju D, Xie Y. Identification of the preferential ubiquitination site and ubiquitin-dependent degradation signal of Rpn4. J Biol Chem. 2006;281:10657-62 pubmed..We further demonstrated that lysine 187 and a proximal acidic domain constitute a portable degradation signal. The implications of our data are discussed. ..
- Sawarynski K, Kaplun A, Tzivion G, Brush G. Distinct activities of the related protein kinases Cdk1 and Ime2. Biochim Biophys Acta. 2007;1773:450-6 pubmed..Examination of other substrates both in vitro and in vivo also revealed differing specificities. These results indicate that Ime2 does not simply replace G1 cyclin-CDK complexes in promoting Sic1 degradation during meiosis. ..
- Chen S, He Q, Greenberg M. Loss of tafazzin in yeast leads to increased oxidative stress during respiratory growth. Mol Microbiol. 2008;68:1061-72 pubmed publisher..Our findings of increased oxidative stress in the taz1Delta mutant during respiratory growth may have important implications for understanding the pathogenesis of Barth syndrome. ..
- Zhong Q, Li G, Gvozdenovic Jeremic J, Greenberg M. Up-regulation of the cell integrity pathway in saccharomyces cerevisiae suppresses temperature sensitivity of the pgs1Delta mutant. J Biol Chem. 2007;282:15946-53 pubmed..These findings demonstrate that functional Pgs1p is essential for cell wall biogenesis and activation of the PKC-Slt2 signaling pathway. ..