Genomes and Genes
Experts and Doctors on saccharomyces cerevisiae in Detroit, Michigan, United States
Locale: Detroit, Michigan, United States
Topic: saccharomyces cerevisiae
Publications103 found, 100 shown here
- Liu Z, Shen J, Carbrey J, Mukhopadhyay R, Agre P, Rosen B. Arsenite transport by mammalian aquaglyceroporins AQP7 and AQP9. Proc Natl Acad Sci U S A. 2002;99:6053-8 pubmed
- Roth A, Feng Y, Chen L, Davis N. The yeast DHHC cysteine-rich domain protein Akr1p is a palmitoyl transferase. J Cell Biol. 2002;159:23-8 pubmed..Finally, our results indicate that palmitoylation within the yeast cell is controlled by multiple PTase specificities. The conserved DHHC-CRD sequence, we propose, is the signature feature of an evolutionarily widespread PTase family. ..
- Ju S, Greenberg M. Valproate disrupts regulation of inositol responsive genes and alters regulation of phospholipid biosynthesis. Mol Microbiol. 2003;49:1595-603 pubmed..Thus, in contrast to the effect of chronic VPA treatment, short-term exposure to VPA abrogated the normal response to inositol depletion of inositol responsive genes and led to aberrant synthesis of phospholipids. ..
- Ju D, Wang L, Mao X, Xie Y. Homeostatic regulation of the proteasome via an Rpn4-dependent feedback circuit. Biochem Biophys Res Commun. 2004;321:51-7 pubmed..This study provides important insights into the mechanism underlying proteasome homeostasis. ..
- Ye C, Bandara W, Greenberg M. Regulation of inositol metabolism is fine-tuned by inositol pyrophosphates in Saccharomyces cerevisiae. J Biol Chem. 2013;288:24898-908 pubmed publisher..We conclude that INO1 transcription is fine-tuned by the synthesis of inositol pyrophosphates, and we propose a model in which modulation of Kcs1 controls INO1 transcription by regulating synthesis of inositol pyrophosphates...
- Ha S, Ju D, Hao W, Xie Y. Rapidly Translated Polypeptides Are Preferred Substrates for Cotranslational Protein Degradation. J Biol Chem. 2016;291:9827-34 pubmed publisher..These results for the first time indicate an inverse correlation between CTPD and cotranslational folding on a proteome scale. The implications of this study with respect to the physiological significance of CTPD are discussed. ..
- Yu W, Daniel J, Mehta D, Maddipati K, Greenberg M. MCK1 is a novel regulator of myo-inositol phosphate synthase (MIPS) that is required for inhibition of inositol synthesis by the mood stabilizer valproate. PLoS ONE. 2017;12:e0182534 pubmed publisher..These findings indicate that VPA-induced MIPS inhibition is Mck1-dependent, and suggest a model that unifies two current hypotheses of the mechanism of action of VPA-inositol depletion and GSK3 inhibition. ..
- Ackerman S, Tzagoloff A. Function, structure, and biogenesis of mitochondrial ATP synthase. Prog Nucleic Acid Res Mol Biol. 2005;80:95-133 pubmed
- Manavathu E, Dimmock J, Vashishtha S, Chandrasekar P. Proton-pumping-ATPase-targeted antifungal activity of a novel conjugated styryl ketone. Antimicrob Agents Chemother. 1999;43:2950-9 pubmed..Vanadate-inhibited hydrolysis of ATP by membrane fractions of C. albicans was completely inhibited by 50 microM NC1175, suggesting that the target of action of NC1175 in these fungi may include H(+)-ATPase...
- Wang Z, Sheluho D, Gatti D, Ackerman S. The alpha-subunit of the mitochondrial F(1) ATPase interacts directly with the assembly factor Atp12p. EMBO J. 2000;19:1486-93 pubmed..These studies provide the basis for a model of F(1) assembly in which Atp12p is released from the alpha-subunit in exchange for a beta-subunit to form the interface that contains the non-catalytic adenine nucleotide-binding site. ..
- Jiang F, Ryan M, Schlame M, Zhao M, Gu Z, Klingenberg M, et al. Absence of cardiolipin in the crd1 null mutant results in decreased mitochondrial membrane potential and reduced mitochondrial function. J Biol Chem. 2000;275:22387-94 pubmed..We propose that CL is required for maintaining the mitochondrial membrane potential and that reduced membrane potential in the absence of CL leads to defects in protein import and other mitochondrial functions. ..
- Grzanowski A, Needleman R, Brusilow W. Immunosuppressant-like effects of phenylbutyrate on growth inhibition of Saccharomyces cerevisiae. Curr Genet. 2002;41:142-9 pubmed..We isolated and characterized a mutant resistant to 1 mM PB and identified the mutant as bul1. A chromosomal BUL1 deletion displayed all phenotypes shown by the PB-resistant mutant. ..
- Mukhopadhyay R, Zhou Y, Rosen B. Directed evolution of a yeast arsenate reductase into a protein-tyrosine phosphatase. J Biol Chem. 2003;278:24476-80 pubmed..The ease by which an arsenate reductase can be converted into a protein-tyrosine phosphatase supports this hypothesis. ..
- He Q, Greenberg M. Post-translational regulation of phosphatidylglycerolphosphate synthase in response to inositol. Mol Microbiol. 2004;53:1243-9 pubmed..This is the first evidence of phosphorylation of a phospholipid biosynthetic enzyme in response to inositol and identifies a new mechanism of inositol-mediated regulation. ..
- Sbrissa D, Ikonomov O, Strakova J, Dondapati R, Mlak K, Deeb R, et al. A mammalian ortholog of Saccharomyces cerevisiae Vac14 that associates with and up-regulates PIKfyve phosphoinositide 5-kinase activity. Mol Cell Biol. 2004;24:10437-47 pubmed..These data demonstrate a major role of the PIKfyve-associated hVac14 protein in activating PIKfyve and thereby regulating PtdIns(3,5)P(2) synthesis and endomembrane homeostasis in mammalian cells. ..
- Ju D, Xu H, Wang X, Xie Y. Ubiquitin-mediated degradation of Rpn4 is controlled by a phosphorylation-dependent ubiquitylation signal. Biochim Biophys Acta. 2007;1773:1672-80 pubmed..This study also suggests that binding to E3 may be only a part of the function of a ubiquitylation signal. ..
- Chen S, Liu D, Finley R, Greenberg M. Loss of mitochondrial DNA in the yeast cardiolipin synthase crd1 mutant leads to up-regulation of the protein kinase Swe1p that regulates the G2/M transition. J Biol Chem. 2010;285:10397-407 pubmed publisher..These results suggest that anionic phospholipids are required for processes that are essential for normal cell division in rho degrees cells. ..
- Zhao M, Rua D, Hajra A, Greenberg M. Enzymatic synthesis of [3H]Cytidine 5'-diphospho-1, 2-diacyl-sn-glycerol. Anal Biochem. 1998;258:48-52 pubmed..This procedure is considerably less labor intensive and less expensive than is chemical synthesis, and the yield is at least 30%. ..
- Ghosh M, Shen J, Rosen B. Pathways of As(III) detoxification in Saccharomyces cerevisiae. Proc Natl Acad Sci U S A. 1999;96:5001-6 pubmed..Disruption of either the ACR3 or YCF1 gene results in sensitivity to arsenite and disruption of both genes produces additive hypersensitivity. Thus, Acr3p and Ycf1p represent separate pathways for the detoxification of arsenite in yeast...
- Robinson K, Koepke J, Kharodawala M, Lopes J. A network of yeast basic helix-loop-helix interactions. Nucleic Acids Res. 2000;28:4460-6 pubmed..Ino2p is the only protein in common with the data presented here. Our finding that Ino4p interacts with five bHLH proteins suggests that Ino4p is likely to be a central player in the coordination of multiple biological processes. ..
- Shao J, Hayashi T, Wang P. Enhanced production of alpha-galactosyl epitopes by metabolically engineered Pichia pastoris. Appl Environ Microbiol. 2003;69:5238-42 pubmed..The Pichia system described here is simple and flexible. This work demonstrates that recombinant P. pastoris is an excellent alternative to Escherichia coli transformants in large-scale synthesis of oligosaccharides. ..
- Zhong J, Zhang H, Stanyon C, Tromp G, Finley R. A strategy for constructing large protein interaction maps using the yeast two-hybrid system: regulated expression arrays and two-phase mating. Genome Res. 2003;13:2691-9 pubmed..We also present a three-dimensional pooling scheme to further increase the efficiency of large-scale two-hybrid analyses. ..
- Weiss A, Delproposto J, Giroux C. High-throughput phenotypic profiling of gene-environment interactions by quantitative growth curve analysis in Saccharomyces cerevisiae. Anal Biochem. 2004;327:23-34 pubmed..Thus, the yeast microculture assay format supports comprehensive applications in toxicogenomics. ..
- Gohil V, Hayes P, Matsuyama S, Schagger H, Schlame M, Greenberg M. Cardiolipin biosynthesis and mitochondrial respiratory chain function are interdependent. J Biol Chem. 2004;279:42612-8 pubmed..This is the first report of regulation of phospholipid biosynthesis by alteration of subcellular compartment pH. ..
- Zhong Q, Greenberg M. Deficiency in mitochondrial anionic phospholipid synthesis impairs cell wall biogenesis. Biochem Soc Trans. 2005;33:1158-61 pubmed..The present review summarizes our analysis of suppression of pgs1Delta growth defects by a mutant that has a loss-of-function mutation in KRE5, a gene involved in cell wall biogenesis. ..
- Ju D, Xie Y. A synthetic defect in protein degradation caused by loss of Ufd4 and Rad23. Biochem Biophys Res Commun. 2006;341:648-52 pubmed..The current work also provides a direction for further investigation of the physiological functions of the UFD pathway. ..
- Ju D, Wang X, Xu H, Xie Y. Genome-wide analysis identifies MYND-domain protein Mub1 as an essential factor for Rpn4 ubiquitylation. Mol Cell Biol. 2008;28:1404-12 pubmed..Together, these data suggest that Mub1 and Ubr2 cooperate to transfer ubiquitin to Rpn4 from Rad6 and that Mub1 may switch from a partner to a substrate of the Ubr2/Rad6 ubiquitin ligase. ..
- Sawarynski K, Najor N, Kepsel A, Brush G. Sic1-induced DNA rereplication during meiosis. Proc Natl Acad Sci U S A. 2009;106:232-7 pubmed publisher..Our results provide strong evidence that CDK activity is required to prevent inappropriate initiation of DNA synthesis before the meiotic divisions. ..
- Chen L, Lopes J. Multiple bHLH proteins regulate CIT2 expression in Saccharomyces cerevisiae. Yeast. 2010;27:345-59 pubmed publisher..Collectively, these results support the model that yeast bHLH proteins coordinate different biological pathways. ..
- Mukundan B, Ansari A. Novel role for mediator complex subunit Srb5/Med18 in termination of transcription. J Biol Chem. 2011;286:37053-7 pubmed publisher..These results strongly suggest that Mediator subunit Srb5/Med18 is required for proper termination of transcription of a subset of genes in budding yeast. ..
- Patil V, Fox J, Gohil V, Winge D, Greenberg M. Loss of cardiolipin leads to perturbation of mitochondrial and cellular iron homeostasis. J Biol Chem. 2013;288:1696-705 pubmed publisher..Increased expression of ATM1 or YAP1 did not rescue the Fe-S defects in crd1?. These findings show for the first time that CL is required for Fe-S biogenesis to maintain mitochondrial and cellular iron homeostasis. ..
- Bartrand A, Iyasu D, Marinco S, Brush G. Evidence of meiotic crossover control in Saccharomyces cerevisiae through Mec1-mediated phosphorylation of replication protein A. Genetics. 2006;172:27-39 pubmed..These results suggest a new function of Mec1 that operates through RPA to locally control reciprocal recombination. ..
- Chen S, Tarsio M, Kane P, Greenberg M. Cardiolipin mediates cross-talk between mitochondria and the vacuole. Mol Biol Cell. 2008;19:5047-58 pubmed publisher..Our results demonstrate the existence of a novel mitochondria-vacuole signaling pathway mediated by CL synthesis. ..
- Schlame M, Zhao M, Rua D, Haldar D, Greenberg M. Kinetic analysis of cardiolipin synthase: a membrane enzyme with two glycerophospholipid substrates. Lipids. 1995;30:633-40 pubmed..It is concluded that a direct association of the metal ion and the enzyme forms the active cardiolipin synthase which has a very high affinity for PtdCMP and a lower affinity for PtdGro. ..
- Wang Z, Schmid K, Ackerman S. The Drosophila gene 2A5 complements the defect in mitochondrial F1-ATPase assembly in yeast lacking the molecular chaperone Atp11p. FEBS Lett. 1999;452:305-8 pubmed..Furthermore, the product of this Drosophila gene was shown to interact with the S. cerevisiae F1 beta subunit in the yeast two-hybrid assay. These results indicate that Atp11p function is conserved in higher eukaryotes. ..
- Mukhopadhyay R, Shi J, Rosen B. Purification and characterization of ACR2p, the Saccharomyces cerevisiae arsenate reductase. J Biol Chem. 2000;275:21149-57 pubmed..This suggests that during the catalytic cycle, Acr2p forms a mixed disulfide with GSH before being reduced by glutaredoxin to regenerate the active Acr2p reductase. ..
- Kaadige M, Lopes J. Opi1p, Ume6p and Sin3p control expression from the promoter of the INO2 regulatory gene via a novel regulatory cascade. Mol Microbiol. 2003;48:823-32 pubmed..We also show that the UME6 gene does not affect the expression of an OPI1-cat reporter. This suggests that Ume6p does not regulate INO2 expression indirectly by regulating OPI1 expression. ..
- Gu Z, Valianpour F, Chen S, Vaz F, Hakkaart G, Wanders R, et al. Aberrant cardiolipin metabolism in the yeast taz1 mutant: a model for Barth syndrome. Mol Microbiol. 2004;51:149-58 pubmed..These results indicate that the taz1Delta mutant is an excellent genetic tool for the study of CL remodelling and may serve as a model system for the study of Barth syndrome. ..
- Chen S, He Q, Greenberg M. Loss of tafazzin in yeast leads to increased oxidative stress during respiratory growth. Mol Microbiol. 2008;68:1061-72 pubmed publisher..Our findings of increased oxidative stress in the taz1Delta mutant during respiratory growth may have important implications for understanding the pathogenesis of Barth syndrome. ..
- Wang X, Xu H, Ha S, Ju D, Xie Y. Proteasomal degradation of Rpn4 in Saccharomyces cerevisiae is critical for cell viability under stressed conditions. Genetics. 2010;184:335-42 pubmed publisher..Rpn4 thus represents an important stress-responsive mediator whose degradation as well as availability are critical for cell survival under stressed conditions. ..
- Rosen B, Tamás M. Arsenic transport in prokaryotes and eukaryotic microbes. Adv Exp Med Biol. 2010;679:47-55 pubmed..In this chapter, we will review the role of AQPs and other proteins in metalloid transport in prokaryotes and eukaryotic microbes. ..
- Roth A, Nelson B, Boone C, Davis N. Asg7p-Ste3p inhibition of pheromone signaling: regulation of the zygotic transition to vegetative growth. Mol Cell Biol. 2000;20:8815-25 pubmed
- Mukhopadhyay R, Rosen B. Arsenate reductases in prokaryotes and eukaryotes. Environ Health Perspect. 2002;110 Suppl 5:745-8 pubmed..The yeast Acr2p has an active site motif HC(X)(5)R that is conserved in protein phosphotyrosine phosphatases and rhodanases, suggesting that these three groups of enzymes may have evolved from an ancestral oxyanion-binding protein. ..
- Ma L, Vaz F, Gu Z, Wanders R, Greenberg M. The human TAZ gene complements mitochondrial dysfunction in the yeast taz1Delta mutant. Implications for Barth syndrome. J Biol Chem. 2004;279:44394-9 pubmed..These findings may shed light on the mitochondrial deficiencies observed in Barth syndrome. ..
- Vaden D, Gohil V, Gu Z, Greenberg M. Separation of yeast phospholipids using one-dimensional thin-layer chromatography. Anal Biochem. 2005;338:162-4 pubmed
- Ju D, Xie Y. Identification of the preferential ubiquitination site and ubiquitin-dependent degradation signal of Rpn4. J Biol Chem. 2006;281:10657-62 pubmed..We further demonstrated that lysine 187 and a proximal acidic domain constitute a portable degradation signal. The implications of our data are discussed. ..
- Sawarynski K, Kaplun A, Tzivion G, Brush G. Distinct activities of the related protein kinases Cdk1 and Ime2. Biochim Biophys Acta. 2007;1773:450-6 pubmed..Examination of other substrates both in vitro and in vivo also revealed differing specificities. These results indicate that Ime2 does not simply replace G1 cyclin-CDK complexes in promoting Sic1 degradation during meiosis. ..
- Zhong Q, Li G, Gvozdenovic Jeremic J, Greenberg M. Up-regulation of the cell integrity pathway in saccharomyces cerevisiae suppresses temperature sensitivity of the pgs1Delta mutant. J Biol Chem. 2007;282:15946-53 pubmed..These findings demonstrate that functional Pgs1p is essential for cell wall biogenesis and activation of the PKC-Slt2 signaling pathway. ..
- Ludlam A, Brunzelle J, Pribyl T, Xu X, Gatti D, Ackerman S. Chaperones of F1-ATPase. J Biol Chem. 2009;284:17138-46 pubmed publisher..However, Atp11p and Atp12p do not resemble alpha or beta, and it is instead the F(1) gamma subunit that initiates the release of the chaperones from alpha and beta and their further assembly into the mature complex. ..
- Ju D, Wang X, Ha S, Fu J, Xie Y. Inhibition of proteasomal degradation of rpn4 impairs nonhomologous end-joining repair of DNA double-strand breaks. PLoS ONE. 2010;5:e9877 pubmed publisher..Inhibition of Rpn4 degradation may result in a concomitant delay of release of Rpn4 and the proteasome from a DSB. This study provides the first evidence for the role of proteasomal degradation of Rpn4 in NHEJ. ..
- Brush G, Najor N, Dombkowski A, Cukovic D, Sawarynski K. Yeast IME2 functions early in meiosis upstream of cell cycle-regulated SBF and MBF targets. PLoS ONE. 2012;7:e31575 pubmed publisher..Thus, Ime2 may functionally resemble Cln3/Cdk1 in promoting S phase entry, or it could play a role even further upstream in the corresponding meiotic cascade. ..
- Yamazaki R, Chau V. Bacterial expression of the Saccharomyces cerevisiae ubiquitin-conjugating enzyme Ubc7. Protein Expr Purif. 1996;7:122-7 pubmed
- Sun B, Chen L, Cao W, Roth A, Davis N. The yeast casein kinase Yck3p is palmitoylated, then sorted to the vacuolar membrane with AP-3-dependent recognition of a YXXPhi adaptin sorting signal. Mol Biol Cell. 2004;15:1397-406 pubmed..Although YXXPhi signals have a well-appreciated role in the adaptin-mediated sorting of mammalian cells, this is the first signal of this class to be identified in yeast. ..
- Liu M, Brusilow W, Needleman R. Activity of the yeast Tat2p tryptophan permease is sensitive to the anti-tumor agent 4-phenylbutyrate. Curr Genet. 2004;46:256-68 pubmed
- Liu Z, Styblo M, Rosen B. Methylarsonous acid transport by aquaglyceroporins. Environ Health Perspect. 2006;114:527-31 pubmed..Identification of MAs(III) as an AQP9 substrate is an important step in understanding physiologic responses to arsenic in mammals, including humans. ..
- Liu Z, Sanchez M, Jiang X, Boles E, Landfear S, Rosen B. Mammalian glucose permease GLUT1 facilitates transport of arsenic trioxide and methylarsonous acid. Biochem Biophys Res Commun. 2006;351:424-30 pubmed..Thus GLUT1 may be a major pathway uptake of both inorganic and methylated arsenicals in erythrocytes or the epithelial cells of the blood-brain barrier, contributing to arsenic-related cardiovascular problems and neurotoxicity. ..
- Ju D, Xu H, Wang X, Xie Y. The transcription activation domain of Rpn4 is separate from its degrons. Int J Biochem Cell Biol. 2010;42:282-6 pubmed publisher..This study provides important information for further understanding the biological functions of Rpn4 and the proteasome system. ..
- Ha S, Ju D, Xie Y. Nuclear import factor Srp1 and its associated protein Sts1 couple ribosome-bound nascent polypeptides to proteasomes for cotranslational degradation. J Biol Chem. 2014;289:2701-10 pubmed publisher..This study unveils a previously unknown role for Srp1 and Sts1 in cotranslational protein degradation and suggests a novel model whereby Srp1 and Sts1 cooperate to couple proteasomes to ribosome-bound nascent polypeptides...
- Joshi A, Fei N, Greenberg M. Get1p and Get2p are required for maintenance of mitochondrial morphology and normal cardiolipin levels. FEMS Yeast Res. 2016;16: pubmed publisher..The genetic interactions observed in this study identify novel physiological modifiers that are required for maintenance of CL levels and mitochondrial morphology. ..
- Yu W, Ye C, Greenberg M. Inositol Hexakisphosphate Kinase 1 (IP6K1) Regulates Inositol Synthesis in Mammalian Cells. J Biol Chem. 2016;291:10437-44 pubmed publisher..This is the first demonstration of IP6K1 as a novel negative regulator of inositol synthesis in mammalian cells. ..
- Gardocki M, Bakewell M, Kamath D, Robinson K, Borovicka K, Lopes J. Genomic analysis of PIS1 gene expression. Eukaryot Cell. 2005;4:604-14 pubmed..Collectively, these studies demonstrate how a collection of genomic and traditional strategies can be implemented to identify a set of genes that affect the regulation of an essential gene. ..
- Chen M, Lopes J. Multiple basic helix-loop-helix proteins regulate expression of the ENO1 gene of Saccharomyces cerevisiae. Eukaryot Cell. 2007;6:786-96 pubmed..These results support the general model that bHLH proteins can coordinate different biological pathways via multiple mechanisms. ..
- Balan V, Miller G, Kaplun L, Balan K, Chong Z, Li F, et al. Life span extension and neuronal cell protection by Drosophila nicotinamidase. J Biol Chem. 2008;283:27810-9 pubmed publisher..Together, our findings establish a life span extending the ability of nicotinamidase in flies and offer a role for nicotinamide-modulating genes in oxidative stress regulated pathways influencing longevity and neuronal cell survival. ..
- Smith M, Petrak M, Boucher P, Barton K, Carter L, Reddy G, et al. Lysine residues at positions 234 and 236 in yeast porin are involved in its assembly into the mitochondrial outer membrane. J Biol Chem. 1995;270:28331-6 pubmed
- Tang K, Finley R, Nie D, Honn K. Identification of 12-lipoxygenase interaction with cellular proteins by yeast two-hybrid screening. Biochemistry. 2000;39:3185-91 pubmed..We also showed that lamin A binds 12-LOX in human tumor cells. These proteins provide the first candidate regulators of 12-LOX. ..
- Clifford D, Marinco S, Brush G. The meiosis-specific protein kinase Ime2 directs phosphorylation of replication protein A. J Biol Chem. 2004;279:6163-70 pubmed..Our studies have identified a novel meiosis-specific reaction that targets a key protein required for DNA replication, repair, and recombination. This pathway could be important in differentiating mitotic and meiotic DNA metabolism. ..
- Wang L, Mao X, Ju D, Xie Y. Rpn4 is a physiological substrate of the Ubr2 ubiquitin ligase. J Biol Chem. 2004;279:55218-23 pubmed..This study not only identified the ubiquitination apparatus for Rpn4 but also unveiled the first physiological substrate of Ubr2. The biological significance of Ubr2-mediated degradation of Rpn4 is also discussed. ..
- Roth A, Papanayotou I, Davis N. The yeast kinase Yck2 has a tripartite palmitoylation signal. Mol Biol Cell. 2011;22:2702-15 pubmed publisher..These soluble proteins have no clear means of accessing membranes and thus may require active capture out of the cytoplasm for palmitoylation by their membrane-localized transferases. ..
- Medler S, Al Husini N, Raghunayakula S, Mukundan B, Aldea A, Ansari A. Evidence for a complex of transcription factor IIB with poly(A) polymerase and cleavage factor 1 subunits required for gene looping. J Biol Chem. 2011;286:33709-18 pubmed publisher..These results suggest that a complex of TFIIB, CF1 subunits, and Pap1 exists in yeast cells. Furthermore, TFIIB interaction with the CF1 complex and Pap1 is crucial for gene looping and transcriptional regulation. ..
- Ha S, Ju D, Xie Y. The N-terminal domain of Rpn4 serves as a portable ubiquitin-independent degron and is recognized by specific 19S RP subunits. Biochem Biophys Res Commun. 2012;419:226-31 pubmed publisher..This is the first time that specific 19S RP subunits have been identified interacting with a Ub-independent degron. This study provides insight into the mechanism by which Ub-independent substrates are recruited to the 26S proteasome. ..
- Banerjee A, Gregori L, Xu Y, Chau V. The bacterially expressed yeast CDC34 gene product can undergo autoubiquitination to form a multiubiquitin chain-linked protein. J Biol Chem. 1993;268:5668-75 pubmed
- Rosen B. Families of arsenic transporters. Trends Microbiol. 1999;7:207-12 pubmed..Yeasts extrude arsenite using Acr3p, a plasma membrane carrier protein, or sequester it in vacuoles as the glutathione conjugate using Ycf1p, an ABC transporter...
- Nakashima H, Grahovac M, Mazzarella R, Fujiwara H, Kitchen J, Threat T, et al. Two novel mouse genes--Nubp2, mapped to the t-complex on chromosome 17, and Nubp1, mapped to chromosome 16--establish a new gene family of nucleotide-binding proteins in eukaryotes. Genomics. 1999;60:152-60 pubmed..Interestingly, both regions are syntenic with human chromosome 16p13.1-p13.3, suggesting that a chromosomal breakage between Nubp2 and Nubp1 probably occurred during the evolution of mouse chromosomes. ..
- Sugiura N, Patel R, Corriveau R. N-methyl-D-aspartate receptors regulate a group of transiently expressed genes in the developing brain. J Biol Chem. 2001;276:14257-63 pubmed..The results suggest that highly specific NMDA receptor-dependent regulation of gene expression plays an important role in the transition from proliferation of neuronal precursors to differentiation of neurons. ..
- Shen J, Hsu C, Kang B, Rosen B, Bhattacharjee H. The Saccharomyces cerevisiae Arr4p is involved in metal and heat tolerance. Biometals. 2003;16:369-78 pubmed..It is proposed that under heat or metal stress, the soluble ATPase becomes membrane-associated, perhaps through interaction with a partner protein, and that this complex is involved in stress tolerance. ..
- Lu L, Roberts G, Simon K, Yu J, Hudson A. Rsf1p, a protein required for respiratory growth of Saccharomyces cerevisiae. Curr Genet. 2003;43:263-72 pubmed..Because of its role in both respiratory growth and mt function, we designate the YMR030 W coding sequence RSF1 (respiration factor 1). ..
- Hancock L, Behta R, Lopes J. Genomic analysis of the Opi- phenotype. Genetics. 2006;173:621-34 pubmed..Regulation in response to inositol is also coordinated with the unfolded protein response (UPR). Consistent with this, several Opi(-) mutants were found to affect the UPR (yhi9, ede1, and vps74). ..
- Zhou J, Zhong Q, Li G, Greenberg M. Loss of cardiolipin leads to longevity defects that are alleviated by alterations in stress response signaling. J Biol Chem. 2009;284:18106-14 pubmed publisher..These findings show for the first time that perturbation of CL synthesis leads to decreased longevity in yeast, which is restored by altering signaling through stress response pathways. ..
- Cook J, Kondapalli K, Rawat S, Childs W, Murugesan Y, Dancis A, et al. Molecular details of the yeast frataxin-Isu1 interaction during mitochondrial Fe-S cluster assembly. Biochemistry. 2010;49:8756-65 pubmed publisher..These results were combined with previously published data to generate a structural model for how the Fe-S cluster protein assembly complex can come together to accomplish Fe-S cluster assembly. ..
- Joshi A, Thompson M, Fei N, Huttemann M, Greenberg M. Cardiolipin and mitochondrial phosphatidylethanolamine have overlapping functions in mitochondrial fusion in Saccharomyces cerevisiae. J Biol Chem. 2012;287:17589-97 pubmed publisher..Taken together, these data demonstrate for the first time in vivo that CL and mitochondrial PE are required to maintain tubular mitochondrial morphology and have overlapping functions in mitochondrial fusion. ..
- Ye C, Lou W, Li Y, Chatzispyrou I, Huttemann M, Lee I, et al. Deletion of the cardiolipin-specific phospholipase Cld1 rescues growth and life span defects in the tafazzin mutant: implications for Barth syndrome. J Biol Chem. 2014;289:3114-25 pubmed publisher..We conclude that transcriptional regulation of Cld1-mediated deacylation of CL influences energy metabolism by modulating the relative contribution of glycolysis and respiration. ..
- Bhagwat A, McClelland M. DNA mismatch correction by Very Short Patch repair may have altered the abundance of oligonucleotides in the E. coli genome. Nucleic Acids Res. 1992;20:1663-8 pubmed..Further, they provide an explanation for the known scarcity of CTAG containing restriction enzyme sites among the genomes of enteric bacteria and identify VSP repair as a force in shaping the sequence composition of bacterial genomes. ..
- Hinton A, Zuiderweg E, Ackerman S. A purified subfragment of yeast Atp11p retains full molecular chaperone activity. J Biol Chem. 2003;278:34110-3 pubmed..Moreover, preliminary 15N-1H heteronuclear single quantum coherence spectra obtained with Atp11pTRNC indicate that the truncated protein is well ordered and amenable to structure determination by nuclear magnetic resonance. ..
- Ju S, Shaltiel G, Shamir A, Agam G, Greenberg M. Human 1-D-myo-inositol-3-phosphate synthase is functional in yeast. J Biol Chem. 2004;279:21759-65 pubmed..Because inositol metabolism may play a key role in the etiology and treatment of bipolar illness, functional conservation of the key enzyme in inositol biosynthesis underscores the power of the yeast model in studies of this disorder. ..
- Zhong Q, Gvozdenovic Jeremic J, Webster P, Zhou J, Greenberg M. Loss of function of KRE5 suppresses temperature sensitivity of mutants lacking mitochondrial anionic lipids. Mol Biol Cell. 2005;16:665-75 pubmed..quot; These findings demonstrated that mitochondrial anionic lipids are required for cellular functions that are essential in cell wall biogenesis, the maintenance of cell integrity, and survival at elevated temperature. ..
- Lu L, Roberts G, Oszust C, Hudson A. The YJR127C/ZMS1 gene product is involved in glycerol-based respiratory growth of the yeast Saccharomyces cerevisiae. Curr Genet. 2005;48:235-46 pubmed..Thus, the product of YJR127C/ZMS1 is involved in transcriptional control for genes in both cellular genetic compartments, many of which specify products required for glycerol-based growth, respiration, and other functions. ..
- Swick L, Kapatos G. A yeast 2-hybrid analysis of human GTP cyclohydrolase I protein interactions. J Neurochem. 2006;97:1447-55 pubmed
- Roth A, Wan J, Bailey A, Sun B, Kuchar J, Green W, et al. Global analysis of protein palmitoylation in yeast. Cell. 2006;125:1003-13 pubmed
- Barnes V, Strunk B, Lee I, Huttemann M, Pile L. Loss of the SIN3 transcriptional corepressor results in aberrant mitochondrial function. BMC Biochem. 2010;11:26 pubmed publisher..The findings that both yeast and Drosophila SIN3 affect mitochondrial activity suggest an evolutionarily conserved role for SIN3 in the control of cellular energy production. ..