Experts and Doctors on operon in Detroit, Michigan, United States


Locale: Detroit, Michigan, United States
Topic: operon

Top Publications

  1. Tisa L, Rosen B. Molecular characterization of an anion pump. The ArsB protein is the membrane anchor for the ArsA protein. J Biol Chem. 1990;265:190-4 pubmed
  2. Rosen B, Weigel U, Monticello R, Edwards B. Molecular analysis of an anion pump: purification of the ArsC protein. Arch Biochem Biophys. 1991;284:381-5 pubmed
    ..The arsC gene was cloned behind a strong promoter and expressed at high levels. The ArsC protein was purified and crystallized. ..
  3. San Francisco M, Tisa L, Rosen B. Identification of the membrane component of the anion pump encoded by the arsenical resistance operon of R-factor R773. Mol Microbiol. 1989;3:15-21 pubmed
    ..In addition to the previously identified ArsA and ArsC proteins, the cells synthesized an inner membrane protein with an apparent mass of 36 kD identified as the ArsB protein. ..
  4. Xu C, Rosen B. Dimerization is essential for DNA binding and repression by the ArsR metalloregulatory protein of Escherichia coli. J Biol Chem. 1997;272:15734-8 pubmed
    ..These results suggest that a core sequence of about 80 residues has all of the information necessary for dimerization, repression, and metal recognition. ..
  5. Hanson B, Runft D, Streeter C, Kumar A, Carion T, Neely M. Functional analysis of the CpsA protein of Streptococcus agalactiae. J Bacteriol. 2012;194:1668-78 pubmed publisher
    ..Taken together, these results suggest that CpsA is a modular protein influencing multiple regulatory functions that may include not only capsule synthesis but also cell wall associated factors...
  6. Pati S, DiSilvestre D, Brusilow W. Regulation of the Escherichia coli uncH gene by mRNA secondary structure and translational coupling. Mol Microbiol. 1992;6:3559-66 pubmed
  7. PALCHAUDHURI S, Rehse S, Hamasha K, Syed T, Kurtovic E, Kurtovic E, et al. Raman spectroscopy of xylitol uptake and metabolism in Gram-positive and Gram-negative bacteria. Appl Environ Microbiol. 2011;77:131-7 pubmed publisher
  8. Fu H, Rosen B, Bhattacharjee H. Biochemical characterization of a novel ArsA ATPase complex from Alkaliphilus metalliredigens QYMF. FEBS Lett. 2010;584:3089-94 pubmed publisher
    ..The results show that AmArsA1-AmArsA2 interaction is needed to form the functional ArsA ATPase. This novel AmArsA1-AmArsA2 complex may provide insight in how it participates with Acr3 in arsenite detoxification...
  9. Lin Y, Yang J, Rosen B. ArsD: an As(III) metallochaperone for the ArsAB As(III)-translocating ATPase. J Bioenerg Biomembr. 2007;39:453-8 pubmed publisher
    ..It is proposed that ArsD residues Cys12, Cys13 and Cys18, but not Cys112, Cys113, Cys119 or Cys120, are required for delivery of As(III) to and activation of the ArsAB pump...

More Information


  1. Qin J, Fu H, Ye J, Bencze K, Stemmler T, Rawlings D, et al. Convergent evolution of a new arsenic binding site in the ArsR/SmtB family of metalloregulators. J Biol Chem. 2007;282:34346-55 pubmed
    ..These results suggest that the As(III)-S(3) binding sites in AfArsR and R773 ArsR arose independently at spatially distinct locations in their three-dimensional structures. ..
  2. Mukhopadhyay R, Rosen B, Phung L, Silver S. Microbial arsenic: from geocycles to genes and enzymes. FEMS Microbiol Rev. 2002;26:311-25 pubmed
    ..Proposed reaction mechanisms in each case involve three cysteine thiols and S-As bond intermediates, so convergent evolution to similar mechanisms has taken place. ..
  3. Shi W, Dong J, Scott R, Ksenzenko M, Rosen B. The role of arsenic-thiol interactions in metalloregulation of the ars operon. J Biol Chem. 1996;271:9291-7 pubmed
  4. Xu C, Shi W, Rosen B. The chromosomal arsR gene of Escherichia coli encodes a trans-acting metalloregulatory protein. J Biol Chem. 1996;271:2427-32 pubmed
    ..In addition, the chromosomally-encoded repressor can regulate expression of the ars operon of plasmid R773, and the R773 repressor can cross-regulate expression from the chromosomal operon. ..
  5. Oden K, Gladysheva T, Rosen B. Arsenate reduction mediated by the plasmid-encoded ArsC protein is coupled to glutathione. Mol Microbiol. 1994;12:301-6 pubmed
    ..Since ArsC-catalysed reduction of arsenate provides high level resistance, physical coupling of the ArsC reaction to efflux of the resulting arsenite is hypothesised. ..
  6. Carlin A, Shi W, Dey S, Rosen B. The ars operon of Escherichia coli confers arsenical and antimonial resistance. J Bacteriol. 1995;177:981-6 pubmed
    ..Expression of the ars genes was inducible by arsenite. By Southern hybridization, the operon was found in all strains of E. coli examined but not in Salmonella typhimurium, Pseudomonas aeruginosa, or Bacillus subtilis. ..
  7. Schemidt R, Hsu D, Deckers Hebestreit G, Altendorf K, Brusilow W. The effects of an atpE ribosome-binding site mutation on the stoichiometry of the c subunit in the F1F0 ATPase of Escherichia coli. Arch Biochem Biophys. 1995;323:423-8 pubmed
    ..Therefore, the stoichiometry of the c subunit assembled into the F1F0 complex appears to be variable, depending on the expression of atpE. ..
  8. Rosen B. The plasmid-encoded arsenical resistance pump: an anion-translocating ATPase. Res Microbiol. 1990;141:336-41 pubmed
    ..This two-subunit enzyme produces resistance to arsenite and antimonite. A third gene, arsC, expands the substrate specificity to allow for arsenate pumping and resistance. ..
  9. Yang H, Cheng J, Finan T, Rosen B, Bhattacharjee H. Novel pathway for arsenic detoxification in the legume symbiont Sinorhizobium meliloti. J Bacteriol. 2005;187:6991-7 pubmed publisher
    ..Thus, AqpS confers arsenate resistance together with ArsC-catalyzed reduction. This is the first report of an aquaglyceroporin with a physiological function in arsenic resistance...
  10. Chen C, Fang M, Majumder A, Wu H. A 72-base pair AT-rich DNA sequence element functions as a bacterial gene silencer. J Biol Chem. 2001;276:9478-85 pubmed
    ..Furthermore, an increased gene-silencing effect was observed with a tandemly repeated AT4 dimer. The possible mechanism of AT4-mediated gene silencing in bacteria is discussed. ..