Genomes and Genes
Experts and Doctors on escherichia coli in Ann Arbor, Michigan, United States
Locale: Ann Arbor, Michigan, United States
Topic: escherichia coli
Publications172 found, 100 shown here
- Veine D, Mulrooney S, Wang P, Williams C. Formation and properties of mixed disulfides between thioredoxin reductase from Escherichia coli and thioredoxin: evidence that cysteine-138 functions to initiate dithiol-disulfide interchange and to accept the reducing equivalent from reduced flavin. Protein Sci. 1998;7:1441-50 pubmed..These results suggest that Cys138 may be the thiol initiating dithiol-disulfide interchange between thioredoxin reductase and thioredoxin...
- Kung F, Garcia G. tRNA-guanine transglycosylase from Escherichia coli: recognition of full-length 'queuine-cognate' tRNAs. FEBS Lett. 1998;431:427-32 pubmed..Interestingly, the yeast tRNAs are essentially equivalent to the E. coli tRNAs as substrates for TGT, indicating that there is nothing intrinsic to the yeast tRNAs that accounts for the absence of queuine in yeast. ..
- Jayasekera M, Foltin S, Olson E, Holler T. Escherichia coli requires the protease activity of FtsH for growth. Arch Biochem Biophys. 2000;380:103-7 pubmed..Since neither mutant would complement the ftsH defect produced in the absence of arabinose, we conclude that the protease function of FtsH is required for bacterial growth. ..
- Williams C, Arscott L, Muller S, Lennon B, Ludwig M, Wang P, et al. Thioredoxin reductase two modes of catalysis have evolved. Eur J Biochem. 2000;267:6110-7 pubmed..P. falciparum is the major causative agent of malaria and it is hoped that the chemical difference between the two high Mr forms may be exploited for drug design. ..
- Peariso K, Zhou Z, Smith A, Matthews R, Penner Hahn J. Characterization of the zinc sites in cobalamin-independent and cobalamin-dependent methionine synthase using zinc and selenium X-ray absorption spectroscopy. Biochemistry. 2001;40:987-93 pubmed
- Trimmer E, Ballou D, Ludwig M, Matthews R. Folate activation and catalysis in methylenetetrahydrofolate reductase from Escherichia coli: roles for aspartate 120 and glutamate 28. Biochemistry. 2001;40:6216-26 pubmed..The mutant enzyme was able to bind CH(3)-H(4)folate, but reduction of the FAD cofactor was not observed. In the NADH-menadione oxidoreductase assay, the mutant demonstrated a 240-fold decrease in activity. ..
- Kittendorf J, Barcomb L, Nonekowski S, Garcia G. tRNA-guanine transglycosylase from Escherichia coli: molecular mechanism and role of aspartate 89. Biochemistry. 2001;40:14123-33 pubmed..These results are most consistent with aspartate 89 acting as a nucleophile in an associative catalytic mechanism...
- Xu D, Enroth C, Lindqvist Y, Ballou D, Massey V. Studies of the mechanism of phenol hydroxylase: effect of mutation of proline 364 to serine. Biochemistry. 2002;41:13627-36 pubmed
- Stevens S, Cai S, Pellecchia M, Zuiderweg E. The solution structure of the bacterial HSP70 chaperone protein domain DnaK(393-507) in complex with the peptide NRLLLTG. Protein Sci. 2003;12:2588-96 pubmed
- Hiniker A, Bardwell J. In vivo substrate specificity of periplasmic disulfide oxidoreductases. J Biol Chem. 2004;279:12967-73 pubmed
- Xu B, Koenig R. An RNA-binding domain in the thyroid hormone receptor enhances transcriptional activation. J Biol Chem. 2004;279:33051-6 pubmed..We conclude that thyroid hormone receptors bind RNA through a novel domain and that the interaction of this domain with SRA, and perhaps other RNAs, enhances thyroid hormone receptor function. ..
- Hernick M, Fierke C. Molecular recognition by Escherichia coli UDP-3-O-(R-3-hydroxymyristoyl)-N-acetylglucosamine deacetylase is modulated by bound metal ions. Biochemistry. 2006;45:14573-81 pubmed
- Meredith T, Aggarwal P, Mamat U, Lindner B, Woodard R. Redefining the requisite lipopolysaccharide structure in Escherichia coli. ACS Chem Biol. 2006;1:33-42 pubmed..The unprecedented nature of a lipid IV(A) OM redefines the requisite LPS structure for viability in E. coli. ..
- Sikora A, Lybarger S, Sandkvist M. Compromised outer membrane integrity in Vibrio cholerae Type II secretion mutants. J Bacteriol. 2007;189:8484-95 pubmed..This suggests that the sugars do not restore the integrity of the outer membrane in the eps mutant strains per se but may provide osmoprotective functions. ..
- Wang X, Hammer N, Chapman M. The molecular basis of functional bacterial amyloid polymerization and nucleation. J Biol Chem. 2008;283:21530-9 pubmed publisher..This work defines the elegant molecular signatures of bacterial amyloid nucleation and polymerization, thereby revealing how nature directs amyloid formation to occur at the correct time and location. ..
- Epstein E, Reizian M, Chapman M. Spatial clustering of the curlin secretion lipoprotein requires curli fiber assembly. J Bacteriol. 2009;191:608-15 pubmed publisher..Finally, we found that efficient fiber assembly was required for the spatial clustering of CsgG. These results suggest a new model where curli fiber formation is spatially coordinated with the CsgG assembly apparatus. ..
- Ren G, Stephan D, Xu Z, Zheng Y, Tang D, Harrison R, et al. Properties of the thioredoxin fold superfamily are modulated by a single amino acid residue. J Biol Chem. 2009;284:10150-9 pubmed publisher..It is isoleucine 75 in the case of thioredoxin. Our findings support the conclusion that a very small percentage of the amino acid residues of thioredoxin-related proteins are capable of dictating the functions of these proteins. ..
- Walk S, Alm E, Gordon D, Ram J, Toranzos G, Tiedje J, et al. Cryptic lineages of the genus Escherichia. Appl Environ Microbiol. 2009;75:6534-44 pubmed publisher..However, our sampling suggests that CIII, CIV, and CV represent environmentally adapted Escherichia lineages that may be more abundant outside the host gastrointestinal tract...
- Cole T, Zhang M, Standiford T, Newstead M, Luther J, Zhang J, et al. IRAK-M modulates expression of IL-10 and cell surface markers CD80 and MHC II after bacterial re-stimulation of tolerized dendritic cells. Immunol Lett. 2012;144:49-59 pubmed publisher..Along with endotoxin, bacterial sonicate is able to induce refractory tolerance in BM-DCs, and IRAK-M plays a role in modulating cell surface expression of MHC class II and CD80 and release of IL-10 during this tolerance. ..
- Dotson G, Dua R, Clemens J, Wooten E, Woodard R. Overproduction and one-step purification of Escherichia coli 3-deoxy-D-manno-octulosonic acid 8-phosphate synthase and oxygen transfer studies during catalysis using isotopic-shifted heteronuclear NMR. J Biol Chem. 1995;270:13698-705 pubmed..The results indicate that the enolic oxygen of the PEP is recovered with the inorganic phosphate, and the C-2 oxygen of KDO 8-P originates from the solvent, H2O. ..
- Chong S, Curnow A, Huston T, Garcia G. tRNA-guanine transglycosylase from Escherichia coli is a zinc metalloprotein. Site-directed mutagenesis studies to identify the zinc ligands. Biochemistry. 1995;34:3694-701 pubmed..These results also suggest that the zinc site is necessary for TGT homotrimer formation and for tRNA binding. ..
- Ganduri Y, Sadda S, Datta M, Jambukeswaran R, Datta P. TdcA, a transcriptional activator of the tdcABC operon of Escherichia coli, is a member of the LysR family of proteins. Mol Gen Genet. 1993;240:395-402 pubmed..Our results suggest that TdcA is related to the LysR family of proteins by common ancestry and, based on its functional role in tdc expression, belongs to the LysR family of transcriptional activators. ..
- Jiang P, Zucker P, Atkinson M, Kamberov E, Tirasophon W, Chandran P, et al. Structure/function analysis of the PII signal transduction protein of Escherichia coli: genetic separation of interactions with protein receptors. J Bacteriol. 1997;179:4342-53 pubmed..Thus, our results support the predictions made from structural studies that the exposed loops of PII and cleft formed at their interface are the sites of regulatory interactions. ..
- Sheppard C, Trimmer E, Matthews R. Purification and properties of NADH-dependent 5, 10-methylenetetrahydrofolate reductase (MetF) from Escherichia coli. J Bacteriol. 1999;181:718-25 pubmed
- Jiang P, Ninfa A. Regulation of autophosphorylation of Escherichia coli nitrogen regulator II by the PII signal transduction protein. J Bacteriol. 1999;181:1906-11 pubmed
- Staker B, Korber P, Bardwell J, Saper M. Structure of Hsp15 reveals a novel RNA-binding motif. EMBO J. 2000;19:749-57 pubmed..An intriguing pattern emerges: stress proteins, ribosomal proteins and tRNA synthetases repeatedly share a conserved motif. This may imply a hitherto unrecognized functional similarity between these three protein classes. ..
- Liepman A, Olsen L. Peroxisomal alanine : glyoxylate aminotransferase (AGT1) is a photorespiratory enzyme with multiple substrates in Arabidopsis thaliana. Plant J. 2001;25:487-98 pubmed..We conclude that Arabidopsis AGT1 is a peroxisomal photorespiratory enzyme that catalyzes transamination reactions with multiple substrates. ..
- Atkinson M, Blauwkamp T, Ninfa A. Context-dependent functions of the PII and GlnK signal transduction proteins in Escherichia coli. J Bacteriol. 2002;184:5364-75 pubmed..In addition, we describe results suggesting that an additional, unknown mechanism may control the cellular level of GlnK. ..
- Palermo E, Kuroda K. Chemical structure of cationic groups in amphiphilic polymethacrylates modulates the antimicrobial and hemolytic activities. Biomacromolecules. 2009;10:1416-28 pubmed publisher..As this value was decreased further to alpha = 0.5, loss of activity was observed. The activities of polymers containing quaternary ammonium groups were shown to be pH-independent. ..
- Fagan R, Palfey B. Roles in binding and chemistry for conserved active site residues in the class 2 dihydroorotate dehydrogenase from Escherichia coli. Biochemistry. 2009;48:7169-78 pubmed publisher..This finding is consistent with previous results that class 2 DHODs use a stepwise mechanism for DHO oxidation. ..
- Patrone J, Yao J, Scott N, Dotson G. Selective inhibitors of bacterial phosphopantothenoylcysteine synthetase. J Am Chem Soc. 2009;131:16340-1 pubmed publisher..A phosphodiester intermediate mimic was the most potent of the compounds synthesized and displayed slow-onset, tight-binding kinetics toward E. faecalis PPCS. ..
- Kim Y, Park J, Reimer T, Baker D, Kawai T, Kumar H, et al. Viral infection augments Nod1/2 signaling to potentiate lethality associated with secondary bacterial infections. Cell Host Microbe. 2011;9:496-507 pubmed publisher..Thus, crosstalk between type I IFNs and Nod1/Nod2 signaling promotes bacterial recognition, but induces harmful effects in the virally infected host. ..
- Thompson A, Bernard S, Skiniotis G, Gestwicki J. Visualization and functional analysis of the oligomeric states of Escherichia coli heat shock protein 70 (Hsp70/DnaK). Cell Stress Chaperones. 2012;17:313-27 pubmed publisher..Together, these studies suggest that DnaK oligomers are composed of ordered multimers that are functionally distinct from monomeric DnaK. Thus, oligomerization of DnaK might be an important step in chaperone cycling. ..
- Nelson A, Walk S, Taube S, Taniuchi M, Houpt E, Wobus C, et al. Disruption of the human gut microbiota following Norovirus infection. PLoS ONE. 2012;7:e48224 pubmed publisher..These results demonstrate that some patients have a disrupted microbiota following Norovirus infection, and therefore may be at elevated risk for long-term health complications. ..
- Cech D, Markin K, Woodard R. Identification of a d-Arabinose-5-Phosphate Isomerase in the Gram-Positive Clostridium tetani. J Bacteriol. 2017;199: pubmed publisher..We have speculated a regulatory role for this API in C. tetani, an organism that does not produce lipopolysaccharide. ..
- Jones P, Cashel M, Glaser G, Neidhardt F. Function of a relaxed-like state following temperature downshifts in Escherichia coli. J Bacteriol. 1992;174:3903-14 pubmed..Our results indicate that the previously reported decrease in the (p)ppGpp level following temperature downshift plays a physiological role in the regulation of gene expression and adaptation for growth at low temperature. ..
- Helling R. icdB mutants of Escherichia coli. J Bacteriol. 1995;177:2592-3 pubmed..icdB mutations map at 16 min, lead to the specific loss of citrate synthase, and are complemented by a prophage containing a gltA+ gene. Thus, they are allelic with gltA. ..
- Reuter K, Chong S, Ullrich F, Kersten H, Garcia G. Serine 90 is required for enzymic activity by tRNA-guanine transglycosylase from Escherichia coli. Biochemistry. 1994;33:7041-6 pubmed..Native PAGE of wild-type and mutant TGTs in the absence and presence of substrate tRNA exhibited band shifts indicating that both mutants retain the ability to bind tRNA.(ABSTRACT TRUNCATED AT 250 WORDS) ..
- Salleh H, Patel M, Woodard R. Essential cysteines in 3-deoxy-D-manno-octulosonic acid 8-phosphate synthase from Escherichia coli: analysis by chemical modification and site-directed mutagenesis. Biochemistry. 1996;35:8942-7 pubmed
- Garcia G, Chong S. Cysteine 265 is in the active site of, but is not essential for catalysis by tRNA-guanine transglycosylase (TGT) from Escherichia coli. J Protein Chem. 1997;16:11-7 pubmed..1966), EMBO J. 15, 2850-2857], which reveals that the residue corresponding to cysteine 265 is distant from the putative catalytic site, but is in the middle of a region of the enzyme surface proposed to bind tRNA. ..
- Gang D, Lavid N, Zubieta C, Chen F, Beuerle T, Lewinsohn E, et al. Characterization of phenylpropene O-methyltransferases from sweet basil: facile change of substrate specificity and convergent evolution within a plant O-methyltransferase family. Plant Cell. 2002;14:505-19 pubmed..This prediction was confirmed by site-directed mutagenesis, in which the appropriate mutants of CVOMT1 (F260S) and EOMT1 (S261F) were produced that exhibited the opposite substrate preference relative to the respective native enzyme. ..
- Osuchowski M, Siddiqui J, Copeland S, Remick D. Sequential ELISA to profile multiple cytokines from small volumes. J Immunol Methods. 2005;302:172-81 pubmed..Given the simplistic validation procedure, the proposed sequential ELISA protocol has a wide potential for further modifications to include other inflammation-related targets. ..
- Nance M, Kreutz B, Tesmer V, Sterne Marr R, Kozasa T, Tesmer J. Structural and functional analysis of the regulator of G protein signaling 2-g?q complex. Structure. 2013;21:438-48 pubmed publisher..RGS subfamilies that do not serve as GAPs for G?q are unlikely to form analogous stabilizing interactions. ..
- Blomfield I, McClain M, Princ J, Calie P, Eisenstein B. Type 1 fimbriation and fimE mutants of Escherichia coli K-12. J Bacteriol. 1991;173:5298-307 pubmed..We believe that these results, which are at odds with a previous study using fim-containing multicopy plasmids, reflect differences in gene copy number. ..
- Granston A, Thompson D, Friedman D. Identification of a second promoter for the metY-nusA-infB operon of Escherichia coli. J Bacteriol. 1990;172:2336-42 pubmed..We demonstrate that P-1 is active in vivo. ..
- Su T, Schweizer H, Oxender D. Carbon-starvation induction of the ugp operon, encoding the binding protein-dependent sn-glycerol-3-phosphate transport system in Escherichia coli. Mol Gen Genet. 1991;230:28-32 pubmed..This conclusion is supported by the fact that pho-independent ugp expression correlated with the presence of the cya and crp gene products. ..
- Craven M, Friedman D. Analysis of the Escherichia coli nusA10(Cs) allele: relating nucleotide changes to phenotypes. J Bacteriol. 1991;173:1485-91 pubmed..These changes alter the reading frame of nusA in a functionally important domain [as identified by the nusA1 and nusA11(Ts) mutations], resulting in an arginine-rich region that may be involved with RNA binding. ..
- Vlahos C, Dekker E. Active-site residues of 2-keto-4-hydroxyglutarate aldolase from Escherichia coli. Bromopyruvate inactivation and labeling of glutamate 45. J Biol Chem. 1990;265:20384-9 pubmed..coli 2-keto-4-hydroxyglutarate aldolase is essential for catalytic activity, most likely acting as the amphoteric proton donor/acceptor that is required as a participant in the overall mechanism of the reaction catalyzed. ..
- Schweizer H, Datta P. Genetic analysis of the tdcABC operon of Escherichia coli K-12. J Bacteriol. 1988;170:5360-3 pubmed..The order of markers in the clockwise direction was dnaG uxaA tdc argG. A tdc deletion was isolated and mapped to this region of the chromosome. By using a tdcB-lacZ fusion the clockwise direction of transcription of tdc was determined. ..
- Peng H, Ding X, Coon M. Isolation and heterologous expression of cloned cDNAs for two rabbit nasal microsomal proteins, CYP2A10 and CYP2A11, that are related to nasal microsomal cytochrome P450 form a. J Biol Chem. 1993;268:17253-60 pubmed..P450 2A10 is generally more active than P450 2A11 and strikingly so in the conversion of testosterone to androstenedione. ..
- Drake S, Koomey M. The product of the pilQ gene is essential for the biogenesis of type IV pili in Neisseria gonorrhoeae. Mol Microbiol. 1995;18:975-86 pubmed..aeruginosa PilQ- mutants by virtue of their expression of rare pilus filaments. Taking these data into account, PilQ is proposed to function in the terminal steps of organelle biogenesis by acting as a pilus channel or pore...
- Miller P, Sulavik M. Overlaps and parallels in the regulation of intrinsic multiple-antibiotic resistance in Escherichia coli. Mol Microbiol. 1996;21:441-8 pubmed..The observed resistance to clinically important antibiotics appears to reflect an overlap with broad-ranged adaptive responses by free-living bacteria to noxious plant materials in their natural environment. ..
- Schauer A, Cheng S, Zheng C, St Pierre L, Alessi D, Hidayetoglu D, et al. The alpha subunit of RNA polymerase and transcription antitermination. Mol Microbiol. 1996;21:839-51 pubmed
- Gong T, Hegeman A, Shin J, Lindberg K, Barald K, Lomax M. Novel genes expressed in the chick otocyst during development: identification using differential display of RNA. Int J Dev Neurosci. 1997;15:585-94 pubmed..These results identified both the avian and human homologues of an evolutionarily conserved gene encoding a small acidic protein of unknown function; however, expression of this gene was not restricted to otocysts. ..
- Helling R. Pathway choice in glutamate synthesis in Escherichia coli. J Bacteriol. 1998;180:4571-5 pubmed..Therefore, substrate availability and/or modulation of enzyme activity are likely to be major determinants of pathway choice in glutamate synthesis. ..
- Muse W, Bender R. The amino-terminal 100 residues of the nitrogen assimilation control protein (NAC) encode all known properties of NAC from Klebsiella aerogenes and Escherichia coli. J Bacteriol. 1999;181:934-40 pubmed..However, MBP-NACE-his6 was completely resistant to cleavage by factor Xa, suggesting an interaction between the C and N termini of this protein. ..
- Lennon B, Williams C, Ludwig M. Crystal structure of reduced thioredoxin reductase from Escherichia coli: structural flexibility in the isoalloxazine ring of the flavin adenine dinucleotide cofactor. Protein Sci. 1999;8:2366-79 pubmed..Distributions of bending angles in published oxidized and reduced flavoenzyme structures are different from those found in studies of free flavins, indicating that the protein environment has a significant effect on bending. ..
- Hamouda T, Baker J. Antimicrobial mechanism of action of surfactant lipid preparations in enteric Gram-negative bacilli. J Appl Microbiol. 2000;89:397-403 pubmed..Understanding the mechanisms of action of these biocidal formulations will help to produce improved formulations with broader spectra of activity. ..
- Goss T, Perez Matos A, Bender R. Roles of glutamate synthase, gltBD, and gltF in nitrogen metabolism of Escherichia coli and Klebsiella aerogenes. J Bacteriol. 2001;183:6607-19 pubmed
- Blauwkamp T, Ninfa A. Physiological role of the GlnK signal transduction protein of Escherichia coli: survival of nitrogen starvation. Mol Microbiol. 2002;46:203-14 pubmed..The specific roles of GlnK during nitrogen starvation were not the result of a distinct function of the protein, as expression of PII from the glnK promoter in cells lacking GlnK restored the wild-type phenotypes. ..
- Atkinson M, Pattaramanon N, Ninfa A. Governor of the glnAp2 promoter of Escherichia coli. Mol Microbiol. 2002;46:1247-57 pubmed
- Winter J, Linke K, Jatzek A, Jakob U. Severe oxidative stress causes inactivation of DnaK and activation of the redox-regulated chaperone Hsp33. Mol Cell. 2005;17:381-92 pubmed..Upon return to nonstress conditions, Hsp33 becomes inactivated while DnaK reactivates and resumes its task to support protein folding. ..
- Park J, Kim Y, Nunez G. RICK promotes inflammation and lethality after gram-negative bacterial infection in mice stimulated with lipopolysaccharide. Infect Immun. 2009;77:1569-78 pubmed publisher..These results demonstrate that RICK contributes to the induction of proinflammatory responses and susceptibility to gram-negative bacteria after exposure to LPS, a condition that is associated with reduced Toll-like receptor signaling. ..
- Yu G, Nguyen T, Guo Y, Schauvinhold I, Auldridge M, Bhuiyan N, et al. Enzymatic functions of wild tomato methylketone synthases 1 and 2. Plant Physiol. 2010;154:67-77 pubmed publisher..In contrast, the 3-ketoacid decarboxylase activity of ShMKS1, which belongs to the alpha/beta-hydrolase fold superfamily, appears to have emerged more recently, possibly within the genus Solanum...
- Berry D, Holder D, Xi C, Raskin L. Comparative transcriptomics of the response of Escherichia coli to the disinfectant monochloramine and to growth conditions inducing monochloramine resistance. Water Res. 2010;44:4924-31 pubmed publisher..This characterization highlights the bacterial responses responsible for decreased sensitivity to monochloramine under different growth conditions. ..
- Minty J, Lesnefsky A, Lin F, Chen Y, Zaroff T, Veloso A, et al. Evolution combined with genomic study elucidates genetic bases of isobutanol tolerance in Escherichia coli. Microb Cell Fact. 2011;10:18 pubmed publisher..coli for isobutanol production. We suggest that rpoS and post-transcriptional regulators, such as hfq, RNA helicases, and sRNAs may be interesting mutagenesis targets for future global phenotype engineering. ..
- Wang D, Hosteen O, Fierke C. ZntR-mediated transcription of zntA responds to nanomolar intracellular free zinc. J Inorg Biochem. 2012;111:173-81 pubmed publisher..A kinetically-controlled transcription model is sufficient to explain the observed regulation of intracellular free zinc concentration by ZntR and ZntA after zinc shock. ..
- Brumbaugh A, Smith S, Mobley H. Immunization with the yersiniabactin receptor, FyuA, protects against pyelonephritis in a murine model of urinary tract infection. Infect Immun. 2013;81:3309-16 pubmed publisher..Together, these iron receptor antigens will facilitate the development of a broadly protective, multivalent UTI vaccine to effectively target diverse strains of UPEC. ..
- Stowe T, Sedgley C, Stowe B, Fenno J. The effects of chlorhexidine gluconate (0.12%) on the antimicrobial properties of tooth-colored ProRoot mineral trioxide aggregate. J Endod. 2004;30:429-31 pubmed..MTA/chlorhexidine showed significantly larger zones of inhibition (p < 0.0002, paired t test). In conclusion, substituting 0.12% chlorhexidine gluconate for water enhanced the antimicrobial activity of tooth-colored ProRoot MTA. ..
- Hondorp E, Matthews R. Oxidative stress inactivates cobalamin-independent methionine synthase (MetE) in Escherichia coli. PLoS Biol. 2004;2:e336 pubmed..In eukaryotes, glutathionylation of key proteins involved in protein synthesis leads to inhibition of translation. Our studies suggest a simpler mechanism is employed by E. coli to achieve the same effect. ..
- Chang L, Bertelsen E, Wisén S, Larsen E, Zuiderweg E, Gestwicki J. High-throughput screen for small molecules that modulate the ATPase activity of the molecular chaperone DnaK. Anal Biochem. 2008;372:167-76 pubmed..Moreover, because this family of chaperones has been linked to numerous diseases, this platform might be used to generate new therapeutic leads. ..
- Datta S, Koutmos M, Pattridge K, Ludwig M, Matthews R. A disulfide-stabilized conformer of methionine synthase reveals an unexpected role for the histidine ligand of the cobalamin cofactor. Proc Natl Acad Sci U S A. 2008;105:4115-20 pubmed publisher..This unanticipated intermodular interaction is expected to play a major role in controlling the distribution of conformers required for the catalytic and the reactivation cycles of the enzyme. ..
- An F, Clewell D. Characterization of the determinant (traB) encoding sex pheromone shutdown by the hemolysin/bacteriocin plasmid pAD1 in Enterococcus faecalis. Plasmid. 1994;31:215-21 pubmed publisher..The deduced product has a mass of 43.7 kDa with the C-terminal third consisting primarily of hydrophobic amino acid residues...
- Roessler B, Nosal J, Smith P, Heidler S, Palella T, Switzer R, et al. Human X-linked phosphoribosylpyrophosphate synthetase superactivity is associated with distinct point mutations in the PRPS1 gene. J Biol Chem. 1993;268:26476-81 pubmed..Normal, N. B., and S. M. PRS1 cDNAs were introduced into Escherichia coli BL21 (DE3)/pLyS, and recombinant N. B. and S. M. PRS1s showed the purine nucleotide feedback resistance phenotypes characteristic of PRS from patients' cells. ..
- Collet J, D Souza J, Jakob U, Bardwell J. Thioredoxin 2, an oxidative stress-induced protein, contains a high affinity zinc binding site. J Biol Chem. 2003;278:45325-32 pubmed..The zinc-free protein conserves its reductase activity. Altogether, our results suggest that the zinc center might play the role of a redox switch, changing a yet to be identified activity. ..
- Yoon M, Patwardhan A, Qiao C, Mansoorabadi S, Menefee A, Reed G, et al. Reaction of adenosylcobalamin-dependent glutamate mutase with 2-thiolglutarate. Biochemistry. 2006;45:11650-7 pubmed..The thioglycolyl radical accumulates on the enzyme, suggesting it is too stable to undergo further steps in the mechanism at a detectable rate. ..
- Lane M, Simms A, Mobley H. complex interplay between type 1 fimbrial expression and flagellum-mediated motility of uropathogenic Escherichia coli. J Bacteriol. 2007;189:5523-33 pubmed..Altogether, these data provide further insight into the complex interplay between type 1 fimbrial expression and flagellum-mediated motility. ..
- Jiang P, Ninfa A. A source of ultrasensitivity in the glutamine response of the bicyclic cascade system controlling glutamine synthetase adenylylation state and activity in Escherichia coli. Biochemistry. 2011;50:10929-40 pubmed publisher..Our results suggest that the AT activity is only bound and activated by completely unmodified PII and that this design is largely responsible for ultrasensitivity of the bicyclic system. ..
- Prongay A, Engelke D, Williams C. Characterization of two active site mutations of thioredoxin reductase from Escherichia coli. J Biol Chem. 1989;264:2656-64 pubmed..The finding of activity in the mutants indicates that the remaining thiol can carry out interchange with the disulfide of thioredoxin, and the resulting mixed disulfide can be reduced by NADPH via the flavin. ..
- Helling R. Why does Escherichia coli have two primary pathways for synthesis of glutamate?. J Bacteriol. 1994;176:4664-8 pubmed..The use of alternative routes for glutamate synthesis implies that the energy cost of biosynthesis may be less when energy is limited than when energy is unlimited. ..
- Matthews R, Sheppard C, Goulding C. Methylenetetrahydrofolate reductase and methionine synthase: biochemistry and molecular biology. Eur J Pediatr. 1998;157 Suppl 2:S54-9 pubmed
- Meredith T, Woodard R. Escherichia coli YrbH is a D-arabinose 5-phosphate isomerase. J Biol Chem. 2003;278:32771-7 pubmed..The value of Keq (Ru5P/A5P) is 0.50 +/- 0.06. Homology searches of the E. coli genome suggest yrbH may be one of multiple genes that encode proteins with API activity. ..
- Walters M, Mobley H. Identification of uropathogenic Escherichia coli surface proteins by shotgun proteomics. J Microbiol Methods. 2009;78:131-5 pubmed publisher..The method described here presents a system to rapidly identify the outer membrane proteome of bacteria, which may prove valuable in vaccine development. ..
- Cheriyan M, Walters M, Kang B, Anzaldi L, Toone E, Fierke C. Directed evolution of a pyruvate aldolase to recognize a long chain acyl substrate. Bioorg Med Chem. 2011;19:6447-53 pubmed publisher..maritima KDPG aldolase. These data indicate that numerous mutations distal from the active site contribute to enhanced 'uniform binding' of the substrates, which is the first step in the evolution of novel catalytic activity. ..