Experts and Doctors on bacterial proteins in East Lansing, Michigan, United States


Locale: East Lansing, Michigan, United States
Topic: bacterial proteins

Top Publications

  1. Simmons L, Kaguni J. The DnaAcos allele of Escherichia coli: hyperactive initiation is caused by substitution of A184V and Y271H, resulting in defective ATP binding and aberrant DNA replication control. Mol Microbiol. 2003;47:755-65 pubmed
    ..These results indicate that a defect in ATP binding results in aberrant control of DNA replication. ..
  2. Denef V, Klappenbach J, Patrauchan M, Florizone C, Rodrigues J, Tsoi T, et al. Genetic and genomic insights into the role of benzoate-catabolic pathway redundancy in Burkholderia xenovorans LB400. Appl Environ Microbiol. 2006;72:585-95 pubmed
    ..Our data further substantiate the expansive network of tightly interconnected and complexly regulated aromatic degradation pathways in LB400. ..
  3. Overbye L, Sandkvist M, Bagdasarian M. Genes required for extracellular secretion of enterotoxin are clustered in Vibrio cholerae. Gene. 1993;132:101-6 pubmed
    ..Gene 123 (1993) 81-86]. Partial reading frames flanking epsM also demonstrated similarity to genes required for extracellular secretion of pullulanase in Klebsiella oxytoca...
  4. Gopalan S, Bauer D, Alfano J, Loniello A, He S, Collmer A. Expression of the Pseudomonas syringae avirulence protein AvrB in plant cells alleviates its dependence on the hypersensitive response and pathogenicity (Hrp) secretion system in eliciting genotype-specific hypersensitive cell death. Plant Cell. 1996;8:1095-105 pubmed
    ..Thus, both stable and transient expression of avrB in Arabidopsis resulted in RPM1-dependent necrosis, and the only demonstrable site of action for AvrB was inside plant cells. ..
  5. Jin Q, Hu W, Brown I, McGhee G, Hart P, Jones A, et al. Visualization of secreted Hrp and Avr proteins along the Hrp pilus during type III secretion in Erwinia amylovora and Pseudomonas syringae. Mol Microbiol. 2001;40:1129-39 pubmed
  6. Zhao Y, He S, Sundin G. The Erwinia amylovora avrRpt2EA gene contributes to virulence on pear and AvrRpt2EA is recognized by Arabidopsis RPS2 when expressed in pseudomonas syringae. Mol Plant Microbe Interact. 2006;19:644-54 pubmed
    ..The results also suggested that although distinct pathogens such as E. amylovora and P. syringae may contain similar effector genes, expression and secretion of these effectors can be under specific regulation by the native pathogen...
  7. Wolk C, Lechno Yossef S, Jäger K. The insertion sequences of Anabaena sp. strain PCC 7120 and their effects on its open reading frames. J Bacteriol. 2010;192:5289-303 pubmed publisher
    ..This observation leads to the expectation that further sequencing projects will extend this and similar analyses. We also propose an adaptive role for poly(A) sequences in ISs...
  8. McNally R, Toth I, Cock P, Pritchard L, Hedley P, Morris J, et al. Genetic characterization of the HrpL regulon of the fire blight pathogen Erwinia amylovora reveals novel virulence factors. Mol Plant Pathol. 2012;13:160-73 pubmed publisher
    ..The construction of deletion mutants of three novel HrpL-regulated genes resulted in the identification of additional virulence factors as well as mutants displaying abnormal motility and biofilm phenotypes...
  9. Wang Q, Kaguni J. Transcriptional repression of the dnaA gene of Escherichia coli by dnaA protein. Mol Gen Genet. 1987;209:518-25 pubmed
    ..These results indicate that transcription of these genes is regulated by binding of dnaA protein to the promoter regions of these genes. ..

More Information

Publications161 found, 100 shown here

  1. Sandkvist M, Morales V, Bagdasarian M. A protein required for secretion of cholera toxin through the outer membrane of Vibrio cholerae. Gene. 1993;123:81-6 pubmed
    ..Sequence similarities in its potential ATP-binding site suggest that the protein may act as an energy provider or signal transducer in the process of extracellular secretion...
  2. Nomura K, DebRoy S, Lee Y, Pumplin N, Jones J, He S. A bacterial virulence protein suppresses host innate immunity to cause plant disease. Science. 2006;313:220-3 pubmed
    ..HopM1 mediates the destruction of AtMIN7 via the host proteasome. Our results illustrate a strategy by which a bacterial pathogen exploits the host proteasome to subvert host immunity and causes infection in plants. ..
  3. Kim J, Mulrooney S, Hausinger R. The UreEF fusion protein provides a soluble and functional form of the UreF urease accessory protein. J Bacteriol. 2006;188:8413-20 pubmed
    ..Analysis of a series of UreEF deletion mutants revealed that the C terminus of UreF is required to form the UreD(EF)G-UreABC complex, while the N terminus of UreF is essential for activation of urease. ..
  4. Wagner T, Mulks M. Identification of the Actinobacillus pleuropneumoniae leucine-responsive regulatory protein and its involvement in the regulation of in vivo-induced genes. Infect Immun. 2007;75:91-103 pubmed
    ..Analysis of expression from the ilvI and antisense cps1AB promoters in wild-type, lrp mutant, and complemented lrp mutant strains indicated that Lrp is required for induction of expression of ilvI under BCAA limitation. ..
  5. Munir M, Wong K, Xagoraraki I. Release of antibiotic resistant bacteria and genes in the effluent and biosolids of five wastewater utilities in Michigan. Water Res. 2011;45:681-93 pubmed publisher
    ..05) were observed in concentrations of ARGs (except tetW) and ARB between the advanced biosolid treatment methods (i.e., anaerobic digestion and lime stabilization) and the conventional dewatering and gravity thickening methods. ..
  6. Carter E, Proshlyakov D, Hausinger R. Apoprotein isolation and activation, and vibrational structure of the Helicobacter mustelae iron urease. J Inorg Biochem. 2012;111:195-202 pubmed publisher
    ..Results from these studies more clearly define the formation and unique properties of the iron urease metallocenter...
  7. Weigand M, Sundin G. General and inducible hypermutation facilitate parallel adaptation in Pseudomonas aeruginosa despite divergent mutation spectra. Proc Natl Acad Sci U S A. 2012;109:13680-5 pubmed publisher
  8. Rodrigues D, Tiedje J. Multi-locus real-time PCR for quantitation of bacteria in the environment reveals Exiguobacterium to be prevalent in permafrost. FEMS Microbiol Ecol. 2007;59:489-99 pubmed
  9. Felczak M, Kaguni J. DnaAcos hyperinitiates by circumventing regulatory pathways that control the frequency of initiation in Escherichia coli. Mol Microbiol. 2009;72:1348-63 pubmed publisher
    ..These results explain how DnaAcos hyperinitiates to interfere with viability. ..
  10. Stanek M, Cooper T, Lenski R. Identification and dynamics of a beneficial mutation in a long-term evolution experiment with Escherichia coli. BMC Evol Biol. 2009;9:302 pubmed publisher
    ..These results show the unpredictability of adaptive evolution, whereas parallel substitutions at other loci in these same populations reveal the predictability. ..
  11. Somvanshi V, Viswanathan P, Jacobs J, Mulks M, Sundin G, Ciche T. The type 2 secretion Pseudopilin, gspJ, is required for multihost pathogenicity of Burkholderia cenocepacia AU1054. Infect Immun. 2010;78:4110-21 pubmed publisher
    ..This study highlights strain specific differences in B. cenocepacia virulence mechanisms important for understanding what enables environmental microbes to function as opportunistic pathogens. ..
  12. Sutter M, Faulkner M, Aussignargues C, Paasch B, Barrett S, Kerfeld C, et al. Visualization of Bacterial Microcompartment Facet Assembly Using High-Speed Atomic Force Microscopy. Nano Lett. 2016;16:1590-5 pubmed publisher
  13. Parrell D, Zhang Y, Olenic S, Kroos L. Bacillus subtilis Intramembrane Protease RasP Activity in Escherichia coli and In Vitro. J Bacteriol. 2017;199: pubmed publisher
    ..coli, providing evidence that both substrates are cleaved by RasP in vivo but, surprisingly, that only RsiW was cleaved in vitro, suggesting that FtsL has an additional requirement. ..
  14. Chodavarapu S, Gomez R, Vicente M, Kaguni J. Escherichia coli Dps interacts with DnaA protein to impede initiation: a model of adaptive mutation. Mol Microbiol. 2008;67:1331-46 pubmed publisher
  15. Lee J, Son B, Viswanathan P, Luethy P, Kroos L. Combinatorial regulation of fmgD by MrpC2 and FruA during Myxococcus xanthus development. J Bacteriol. 2011;193:1681-9 pubmed publisher
    ..This would explain why fmgD transcription begins later during development and is more dependent on C-signaling than transcription of fmgA and fmgBC. ..
  16. Dong G, Vieille C, Zeikus J. Cloning, sequencing, and expression of the gene encoding amylopullulanase from Pyrococcus furiosus and biochemical characterization of the recombinant enzyme. Appl Environ Microbiol. 1997;63:3577-84 pubmed
    ..5. Ca2+ increased the enzyme activity, thermostability, and substrate affinity. The enzyme was highly resistant to chemical denaturing reagents, and its activity increased up to twofold in the presence of surfactants. ..
  17. Boer J, Hausinger R. Klebsiella aerogenes UreF: identification of the UreG binding site and role in enhancing the fidelity of urease activation. Biochemistry. 2012;51:2298-308 pubmed publisher
    ..Further experiments with these complexes provided evidence that UreF gates the GTPase activity of UreG to enhance the fidelity of urease metallocenter assembly, especially in the presence of the noncognate metal Zn. ..
  18. Gulati M, Jain N, Anand B, Prakash B, Britton R. Mutational analysis of the ribosome assembly GTPase RbgA provides insight into ribosome interaction and ribosome-stimulated GTPase activation. Nucleic Acids Res. 2013;41:3217-27 pubmed publisher
    ..Finally, our results support a model in which the GTPase activity of RbgA directly participates in the maturation of the large subunit rather than solely promoting dissociation of RbgA from the 50S subunit. ..
  19. Karkman A, Johnson T, Lyra C, Stedtfeld R, Tamminen M, Tiedje J, et al. High-throughput quantification of antibiotic resistance genes from an urban wastewater treatment plant. FEMS Microbiol Ecol. 2016;92: pubmed publisher
    ..Wastewater discharge enriched only a few genes, namely Tn25 type transposase gene and clinical class 1 integrons, in the sediment near the discharge pipe, but those enriched genes may indicate a potential for horizontal gene transfer. ..
  20. Mathupala S, Lowe S, Podkovyrov S, Zeikus J. Sequencing of the amylopullulanase (apu) gene of Thermoanaerobacter ethanolicus 39E, and identification of the active site by site-directed mutagenesis. J Biol Chem. 1993;268:16332-44 pubmed
    ..These findings substantiate amylopullulanase as a new type of amylosaccharidase...
  21. Konstantinidis K, Ramette A, Tiedje J. Toward a more robust assessment of intraspecies diversity, using fewer genetic markers. Appl Environ Microbiol. 2006;72:7286-93 pubmed
    ..Our results are reproducible within the Salmonella, Burkholderia, and Shewanella groups and therefore are expected to have general applicability for microevolution studies, including metagenomic surveys. ..
  22. Speth E, Imboden L, Hauck P, He S. Subcellular localization and functional analysis of the Arabidopsis GTPase RabE. Plant Physiol. 2009;149:1824-37 pubmed publisher
    ..RabE down-regulation did not affect plant susceptibility to pathogenic P. syringae bacteria; conversely, expression of the constitutively active RabE1d-Q74L enhanced plant defenses, conferring resistance to P. syringae infection. ..
  23. Zhang Y, Halder S, Kerr R, Parrell D, Ruotolo B, Kroos L. Complex Formed between Intramembrane Metalloprotease SpoIVFB and Its Substrate, Pro-σK. J Biol Chem. 2016;291:10347-62 pubmed publisher
  24. Johnson T, Stedtfeld R, Wang Q, Cole J, Hashsham S, Looft T, et al. Clusters of Antibiotic Resistance Genes Enriched Together Stay Together in Swine Agriculture. MBio. 2016;7:e02214-15 pubmed publisher
  25. Sutton M, Kaguni J. Threonine 435 of Escherichia coli DnaA protein confers sequence-specific DNA binding activity. J Biol Chem. 1997;272:23017-24 pubmed
  26. Zarzycki J, Kerfeld C. The crystal structures of the tri-functional Chloroflexus aurantiacus and bi-functional Rhodobacter sphaeroides malyl-CoA lyases and comparison with CitE-like superfamily enzymes and malate synthases. BMC Struct Biol. 2013;13:28 pubmed publisher
    ..Further studies involving site-directed mutagenesis based on these structures may be required to solve this puzzling question. ..
  27. Mittal S, Kroos L. A combination of unusual transcription factors binds cooperatively to control Myxococcus xanthus developmental gene expression. Proc Natl Acad Sci U S A. 2009;106:1965-70 pubmed publisher
    ..We propose that this unusual mechanism of combinatorial control allows coordination of morphogenetic C-signaling with starvation signaling and cell death, determining spatiotemporal gene expression and cell fate. ..
  28. Kroos L, Zhang B, Ichikawa H, Yu Y. Control of sigma factor activity during Bacillus subtilis sporulation. Mol Microbiol. 1999;31:1285-94 pubmed
    ..Together, the mechanisms controlling sigma factor activity ensure that genes are expressed at the proper time and level in each cell type. ..
  29. Liu J, Qin L, Ferguson Miller S. Crystallographic and online spectral evidence for role of conformational change and conserved water in cytochrome oxidase proton pump. Proc Natl Acad Sci U S A. 2011;108:1284-9 pubmed publisher
    ..A mechanism is described that explains the conformational change and the incomplete response of the D-path mutant. ..
  30. Schwartz S, Black T, Jager K, Panoff J, Wolk C. Regulation of an osmoticum-responsive gene in Anabaena sp. strain PCC 7120. J Bacteriol. 1998;180:6332-7 pubmed
  31. Snyder L, Blight S, Auchtung J. Regulation of translation of the head protein of T4 bacteriophage by specific binding of EF-Tu to a leader sequence. J Mol Biol. 2003;334:349-61 pubmed
  32. Bakermans C, Nealson K. Relationship of critical temperature to macromolecular synthesis and growth yield in Psychrobacter cryopegella. J Bacteriol. 2004;186:2340-5 pubmed
    ..Identifying the specific processes which result in T(critical) will be vital to understanding both low-temperature growth and growth over a wide range of temperatures...
  33. Sloup R, Konal A, Severin G, Korir M, Bagdasarian M, Bagdasarian M, et al. Cyclic Di-GMP and VpsR Induce the Expression of Type II Secretion in Vibrio cholerae. J Bacteriol. 2017;199: pubmed publisher
    ..Here, we show that the widely conserved second messenger cyclic di-GMP (c-di-GMP) upregulates expression of the eps genes encoding the T2SS in the pathogen V. cholerae via the c-di-GMP-dependent transcription factor VpsR. ..
  34. Mah M, Maher V, Thomas H, Reid T, King C, McCormick J. Mutations induced by aminofluorene-DNA adducts during replication in human cells. Carcinogenesis. 1989;10:2321-8 pubmed
    ..The most prominent mutation hot spot was also the most prominent hot spot for adduct formation as judged by the frequency of termination of in vitro polymerization by the Klenow fragment on N-AcO-TFA-AF-treated plasmids...
  35. Priemé A, Braker G, Tiedje J. Diversity of nitrite reductase (nirK and nirS) gene fragments in forested upland and wetland soils. Appl Environ Microbiol. 2002;68:1893-900 pubmed
  36. Zhou R, Kroos L. BofA protein inhibits intramembrane proteolysis of pro-sigmaK in an intercompartmental signaling pathway during Bacillus subtilis sporulation. Proc Natl Acad Sci U S A. 2004;101:6385-90 pubmed
    ..The approach described herein should be applicable to studies of other RIP events and amenable to developing in vitro assays for I-Clips. ..
  37. Schaefer L, Uicker W, Wicker Planquart C, Foucher A, Jault J, Britton R. Multiple GTPases participate in the assembly of the large ribosomal subunit in Bacillus subtilis. J Bacteriol. 2006;188:8252-8 pubmed publisher
    ..Our results indicate that, as has been demonstrated for Saccharomyces cerevisiae ribosome biogenesis, bacterial 50S ribosome assembly requires the function of multiple essential GTPases...
  38. Ludlam A, McNatt M, Carr K, Kaguni J. Essential amino acids of Escherichia coli DnaC protein in an N-terminal domain interact with DnaB helicase. J Biol Chem. 2001;276:27345-53 pubmed
    ..Bond, M. W., Miyajima, A., Kobori, J., and Arai, K. (1987) J. Biol. Chem. 262, 10475-10480). ..
  39. Bakermans C, Tollaksen S, Giometti C, Wilkerson C, Tiedje J, Thomashow M. Proteomic analysis of Psychrobacter cryohalolentis K5 during growth at subzero temperatures. Extremophiles. 2007;11:343-54 pubmed
    ..In summary, these data suggest that low-temperature stresses continue to intensify as growth temperatures decrease to -4 degrees C. ..
  40. Achila D, Gulati M, Jain N, Britton R. Biochemical characterization of ribosome assembly GTPase RbgA in Bacillus subtilis. J Biol Chem. 2012;287:8417-23 pubmed publisher
    ..These results support a model in which RbgA promotes a late step in ribosome biogenesis and that one role of GTP hydrolysis is to stimulate dissociation of RbgA from the ribosome. ..
  41. Sutton M, Kaguni J. Novel alleles of the Escherichia coli dnaA gene. J Mol Biol. 1997;271:693-703 pubmed
    ..Many of the missense mutations mapping to the C-terminal 61 residues were inactive for replication from the pSC101 origin. These are defective in DNA binding. Mutations that mapped elsewhere were temperature-sensitive. ..
  42. Martin P, Shea R, Mulks M. Identification of a plasmid-encoded gene from Haemophilus ducreyi which confers NAD independence. J Bacteriol. 2001;183:1168-74 pubmed publisher
    ..Identification of a function for this gene in the Pasteurellaceae should help to elucidate the role of its homologues in other species...
  43. Large T, Walk S, Whittam T. Variation in acid resistance among shiga toxin-producing clones of pathogenic Escherichia coli. Appl Environ Microbiol. 2005;71:2493-500 pubmed
  44. Riekhof W, Andre C, Benning C. Two enzymes, BtaA and BtaB, are sufficient for betaine lipid biosynthesis in bacteria. Arch Biochem Biophys. 2005;441:96-105 pubmed
    ..Comparative analysis of RsBtaA and its bacterial orthologs revealed a motif with similarity to the AdoMet binding pocket of methyltransferases, and allowed the prediction of residues involved in substrate binding. ..
  45. Viswanathan P, Ueki T, Inouye S, Kroos L. Combinatorial regulation of genes essential for Myxococcus xanthus development involves a response regulator and a LysR-type regulator. Proc Natl Acad Sci U S A. 2007;104:7969-74 pubmed
    ..FruA and LadA act on a regulatory region spanning >400 bp to bring about proper temporal and spatial expression of the dev operon, resembling the regulation of developmental genes in multicellular eukaryotes. ..
  46. Zwiesler Vollick J, Plovanich Jones A, Nomura K, Bandyopadhyay S, Joardar V, Kunkel B, et al. Identification of novel hrp-regulated genes through functional genomic analysis of the Pseudomonas syringae pv. tomato DC3000 genome. Mol Microbiol. 2002;45:1207-18 pubmed
    ..An AvrRpt2-based type III translocation assay provides evidence that some of the hrpS-regulated novel genes encode putative effector proteins...
  47. Du Y, Arvidson C. Identification of ZipA, a signal recognition particle-dependent protein from Neisseria gonorrhoeae. J Bacteriol. 2003;185:2122-30 pubmed
  48. Mulrooney S, Ward S, Hausinger R. Purification and properties of the Klebsiella aerogenes UreE metal-binding domain, a functional metallochaperone of urease. J Bacteriol. 2005;187:3581-5 pubmed
    ..This domain was purified and shown to exhibit metal-binding properties similar to those of UreE lacking only the His-rich tail. ..
  49. Hassler B, Kohli N, Zeikus J, Lee I, Worden R. Renewable dehydrogenase-based interfaces for bioelectronic applications. Langmuir. 2007;23:7127-33 pubmed
  50. Chang Z, Kuchar J, Hausinger R. Chemical cross-linking and mass spectrometric identification of sites of interaction for UreD, UreF, and urease. J Biol Chem. 2004;279:15305-13 pubmed
  51. Kroos L. Bacterial development in the fast lane. J Bacteriol. 2008;190:4373-6 pubmed publisher
  52. Parker R, Laut C, Gaddy J, Zadoks R, Davies H, Manning S. Association between genotypic diversity and biofilm production in group B Streptococcus. BMC Microbiol. 2016;16:86 pubmed publisher
    ..The findings herein also demonstrate that biofilms must be considered in the treatment of pregnant women, particularly for women with heavy GBS colonization. ..
  53. Achkar J, Xian M, Zhao H, Frost J. Biosynthesis of phloroglucinol. J Am Chem Soc. 2005;127:5332-3 pubmed
  54. Fowler J, Wulff C, Straley S, Brubaker R. Growth of calcium-blind mutants of Yersinia pestis at 37 degrees C in permissive Ca2+-deficient environments. Microbiology. 2009;155:2509-21 pubmed publisher
    ..The Ca(2+)-blind yopD phenotype was fully suppressed in a Ca(2+)-independent background lacking the injectisome-associated inner-membrane component YscV but not peripheral YscK, suggesting that the core translocon energizes YopD. ..
  55. Sambanthamoorthy K, Sloup R, Parashar V, Smith J, Kim E, Semmelhack M, et al. Identification of small molecules that antagonize diguanylate cyclase enzymes to inhibit biofilm formation. Antimicrob Agents Chemother. 2012;56:5202-11 pubmed publisher
    ..cholerae, one of which also inhibits biofilm formation by Pseudomonas aeruginosa in a continuous-flow system. These molecules represent the first compounds described that are able to inhibit DGC activity to prevent biofilm formation. ..
  56. Brubaker R. Interleukin-10 and inhibition of innate immunity to Yersiniae: roles of Yops and LcrV (V antigen). Infect Immun. 2003;71:3673-81 pubmed
  57. Awai K, Wolk C. Identification of the glycosyl transferase required for synthesis of the principal glycolipid characteristic of heterocysts of Anabaena sp. strain PCC 7120. FEMS Microbiol Lett. 2007;266:98-102 pubmed
    ..An insertional mutant of all5341 synthesized only a nonglycosylated form of heterocyst envelope glycolipid, and lacked a glycolipid layer. All5341 appears to be the transferase required to glycosylate the glycolipid aglycone. ..
  58. Hayden H, Matamouros S, Hager K, Brittnacher M, Rohmer L, Radey M, et al. Genomic Analysis of Salmonella enterica Serovar Typhimurium Characterizes Strain Diversity for Recent U.S. Salmonellosis Cases and Identifies Mutations Linked to Loss of Fitness under Nitrosative and Oxidative Stress. MBio. 2016;7:e00154 pubmed publisher
    ..Additionally, defining simple correlates of pathogenesis may have predictive value for patient outcome. ..
  59. Klug R, Benning C. Two enzymes of diacylglyceryl-O-4'-(N,N,N,-trimethyl)homoserine biosynthesis are encoded by btaA and btaB in the purple bacterium Rhodobacter sphaeroides. Proc Natl Acad Sci U S A. 2001;98:5910-5 pubmed publisher
    ..Because the equivalent reactions have been proposed for different eukaryotes, it seems likely that orthologs of btaA/btaB may be present in other betaine lipid-containing organisms...
  60. Tian M, Chaudhry F, Ruzicka D, Meagher R, Staiger C, Day B. Arabidopsis actin-depolymerizing factor AtADF4 mediates defense signal transduction triggered by the Pseudomonas syringae effector AvrPphB. Plant Physiol. 2009;150:815-24 pubmed publisher
    ..Collectively, this study identifies AtADF4 as a novel component of the plant defense signaling pathway and provides strong evidence for actin dynamics as a primary component that orchestrates plant defenses against P. syringae. ..
  61. He S, Nomura K, Whittam T. Type III protein secretion mechanism in mammalian and plant pathogens. Biochim Biophys Acta. 2004;1694:181-206 pubmed
    ..Here, we summarize the most salient aspects of the TTSS, with special emphasis on recent findings. ..
  62. Riekhof W, Sears B, Benning C. Annotation of genes involved in glycerolipid biosynthesis in Chlamydomonas reinhardtii: discovery of the betaine lipid synthase BTA1Cr. Eukaryot Cell. 2005;4:242-52 pubmed
    ..Site-directed mutagenesis of the active sites of the two domains of BTA1Cr allowed us to study their activities separately, demonstrating directly their functional homology to the bacterial orthologs BtaARs and BtaBRs. ..
  63. Yoder Himes D, Kroos L. Regulation of the Myxococcus xanthus C-signal-dependent Omega4400 promoter by the essential developmental protein FruA. J Bacteriol. 2006;188:5167-76 pubmed
    ..Also, the highly localized effects of mutations between bp -86 and -77 on DNA binding in vitro, together with recently published footprints, allow us to predict a consensus binding site of GTCG/CGA/G for the FruA DNA-binding domain. ..
  64. Lechno Yossef S, Fan Q, Wojciuch E, Wolk C. Identification of ten Anabaena sp. genes that under aerobic conditions are required for growth on dinitrogen but not for growth on fixed nitrogen. J Bacteriol. 2011;193:3482-9 pubmed publisher
    ..Elucidation of the roles of these genes should enhance understanding of heterocyst biology. ..
  65. Hwang D, Kaguni J. Purification and characterization of the dnaA46 gene product. J Biol Chem. 1988;263:10625-32 pubmed
    ..In contrast with the wild type protein, DNA synthesis dependent on dnaA46 protein showed a pronounced lag before incorporation of deoxyribonucleotides. ..
  66. Cheek J, Broderick J. Adenosylmethionine-dependent iron-sulfur enzymes: versatile clusters in a radical new role. J Biol Inorg Chem. 2001;6:209-26 pubmed
    ..The purpose of this review is to present the current state of knowledge of this newly emerging group of Fe-S/AdoMet enzymes. ..
  67. Parnell J, Denef V, Park J, Tsoi T, Tiedje J. Environmentally relevant parameters affecting PCB degradation: carbon source- and growth phase-mitigated effects of the expression of the biphenyl pathway and associated genes in Burkholderia xenovorans LB400. Biodegradation. 2010;21:147-56 pubmed publisher
  68. Son B, Liu Y, Kroos L. Combinatorial regulation by MrpC2 and FruA involves three sites in the fmgE promoter region during Myxococcus xanthus development. J Bacteriol. 2011;193:2756-66 pubmed publisher
  69. Schmidt B, McCracken J, Ferguson Miller S. A discrete water exit pathway in the membrane protein cytochrome c oxidase. Proc Natl Acad Sci U S A. 2003;100:15539-42 pubmed
    ..The significant broadening of the Mn2+ signal after the limited number of turnovers strongly suggests that the water exits the protein by means of one discrete pathway, not by random diffusion. ..
  70. Bordowitz J, Montgomery B. Photoregulation of cellular morphology during complementary chromatic adaptation requires sensor-kinase-class protein RcaE in Fremyella diplosiphon. J Bacteriol. 2008;190:4069-74 pubmed publisher
    ..Detailed microscopic and biochemical analyses indicate that RcaE is required for the photoregulation of cell and filament morphologies of F. diplosiphon in response to red and green light. ..
  71. Katharios Lanwermeyer S, Rakic Martinez M, Elhanafi D, Ratani S, Tiedje J, Kathariou S. Coselection of cadmium and benzalkonium chloride resistance in conjugative transfers from nonpathogenic Listeria spp. to other Listeriae. Appl Environ Microbiol. 2012;78:7549-56 pubmed publisher
    ..The results suggest that nonpathogenic Listeria spp. may behave as reservoirs for disinfectant and heavy metal resistance genes for other listeriae, including the pathogenic species L. monocytogenes. ..
  72. Black T, Wolk C. Analysis of a Het- mutation in Anabaena sp. strain PCC 7120 implicates a secondary metabolite in the regulation of heterocyst spacing. J Bacteriol. 1994;176:2282-92 pubmed
    ..The presence of hetN, as the only ORF, on a replicating plasmid suppresses heterocyst formation in wild-type cells, whereas the additional presence of hetI alleviates this effect. ..
  73. Benning C, Somerville C. Isolation and genetic complementation of a sulfolipid-deficient mutant of Rhodobacter sphaeroides. J Bacteriol. 1992;174:2352-60 pubmed
    ..The putative sqdA gene product is a protein with a molecular mass of 33.6 kDa that has no sequence similarity to any enzyme of known function. ..
  74. Sutton M, Kaguni J. Novel alleles of the Escherichia coli dnaA gene are defective in replication of pSC101 but not of oriC. J Bacteriol. 1995;177:6657-65 pubmed
    ..We speculate that one or more of these pSC101-specific mutants are defective in interaction with pSC101 RepA protein, which is also required for initiation of plasmid DNA replication. ..
  75. Kim J, Mulrooney S, Hausinger R. Biosynthesis of active Bacillus subtilis urease in the absence of known urease accessory proteins. J Bacteriol. 2005;187:7150-4 pubmed publisher
    ..Additional investigations of the system lead to the suggestion that B. subtilis may use unidentified accessory proteins for in vivo urease activation...
  76. Raghunathan K, Harris P, Spurbeck R, ARVIDSON C, Arvidson D. Crystal structure of an efficacious gonococcal adherence inhibitor: an enolase from Lactobacillus gasseri. FEBS Lett. 2014;588:2212-6 pubmed publisher
    ..Determined using molecular replacement to 2.08Å, the structure has a flexible and surface exposed catalytic loop containing lysines, and may play a role in the inhibitory function. ..
  77. Fernández Piñas F, Leganés F, Wolk C. A third genetic locus required for the formation of heterocysts in Anabaena sp. strain PCC 7120. J Bacteriol. 1994;176:5277-83 pubmed
    ..Filaments of strain P6 and of the wild-type strain, when bearing the complementing fragment on a pDU1-based plasmid, showed an increased frequency of clustered heterocysts compared with that of the wild-type strain. ..
  78. Stigter J, Schneider M, de Bruijn F. Azorhizobium caulinodans nitrogen fixation (nif/fix) gene regulation: mutagenesis of the nifA -24/-12 promoter element, characterization of a ntrA(rpoN) gene, and derivation of a model. Mol Plant Microbe Interact. 1993;6:238-52 pubmed
    ..caulinodans nif genes, but not the central nif regulatory gene nifA. Based on these results, a new model for the regulation of nif/fix gene expression in A. caulinodans is proposed. ..
  79. Sutton M, Kaguni J. The Escherichia coli dnaA gene: four functional domains. J Mol Biol. 1997;274:546-61 pubmed
  80. Foster G, McGhee G, Jones A, Sundin G. Nucleotide sequences, genetic organization, and distribution of pEU30 and pEL60 from Erwinia amylovora. Appl Environ Microbiol. 2004;70:7539-44 pubmed
    ..The gene content of pEU30 resembled plasmids found in plant-associated bacteria, while that of pEL60 was most similar to IncL/M plasmids inhabiting enteric bacteria. ..
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    ..Based on similarities in the replication process between prokaryotes and eukaryotes, these results suggest that a similar mechanism may load the eukaryotic replicative helicase. ..
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    ..Interested users are invited to download a beta version (available on iOS or Android), submit AR information using the application, and provide feedback on current and prospective functionalities. ..
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    ..Since the assembly of T4 heads is thought to occur on the inner face of the inner membrane, we propose that gplit interferes with a normal regulation which coordinates the synthesis of proteins and the assembly of T4 heads. ..
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    ..Transcription of human snRNA genes is activated by these direct protein-protein interactions. ..
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    ..Our results show that the mutant fails to form a stable oligomeric DnaA-oriC complex, which is required for the loading of DnaB. ..
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    ..g. oxidative stress, starvation). We also conclude based on sequence, physiological and expression analyses that this regulator is more appropriately termed Fnr and recommend this descriptor be used in future publications...
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    ..In this issue of Molecular Microbiology, work by Nissan et al. sheds light onto the molecular-genetic patterns involved in determining host specificity and pathogen virulence in the Pantoea-gypsophila interaction. ..
  91. Carr K, Kaguni J. Stoichiometry of DnaA and DnaB protein in initiation at the Escherichia coli chromosomal origin. J Biol Chem. 2001;276:44919-25 pubmed
    ..That only two DnaB hexamers are bound, one for each replication fork, suggests that the binding of additional molecules of DnaA in forming the prepriming complex restricts the loading of additional DnaB hexamers that can bind at oriC. ..
  92. Reusch R, Shabalin O, Crumbaugh A, Wagner R, Schroder O, Wurm R. Posttranslational modification of E. coli histone-like protein H-NS and bovine histones by short-chain poly-(R)-3-hydroxybutyrate (cPHB). FEBS Lett. 2002;527:319-22 pubmed
    ..The molecular characteristics of cPHB and its presence in histone-like and histone proteins of diverse organisms suggest it may play a role in DNA binding and/or DNA organization. ..