Experts and Doctors on arsenites in Detroit, Michigan, United States

Summary

Locale: Detroit, Michigan, United States
Topic: arsenites

Top Publications

  1. Rosen B, Weigel U, Monticello R, Edwards B. Molecular analysis of an anion pump: purification of the ArsC protein. Arch Biochem Biophys. 1991;284:381-5 pubmed
    ..The arsC gene was cloned behind a strong promoter and expressed at high levels. The ArsC protein was purified and crystallized. ..
  2. Rosen B. The plasmid-encoded arsenical resistance pump: an anion-translocating ATPase. Res Microbiol. 1990;141:336-41 pubmed
    ..This two-subunit enzyme produces resistance to arsenite and antimonite. A third gene, arsC, expands the substrate specificity to allow for arsenate pumping and resistance. ..
  3. Carlin A, Shi W, Dey S, Rosen B. The ars operon of Escherichia coli confers arsenical and antimonial resistance. J Bacteriol. 1995;177:981-6 pubmed
    ..Expression of the ars genes was inducible by arsenite. By Southern hybridization, the operon was found in all strains of E. coli examined but not in Salmonella typhimurium, Pseudomonas aeruginosa, or Bacillus subtilis. ..
  4. Shi W, Dong J, Scott R, Ksenzenko M, Rosen B. The role of arsenic-thiol interactions in metalloregulation of the ars operon. J Biol Chem. 1996;271:9291-7 pubmed
  5. Ghosh M, Shen J, Rosen B. Pathways of As(III) detoxification in Saccharomyces cerevisiae. Proc Natl Acad Sci U S A. 1999;96:5001-6 pubmed
    ..Disruption of either the ACR3 or YCF1 gene results in sensitivity to arsenite and disruption of both genes produces additive hypersensitivity. Thus, Acr3p and Ycf1p represent separate pathways for the detoxification of arsenite in yeast...
  6. Tisa L, Rosen B. Molecular characterization of an anion pump. The ArsB protein is the membrane anchor for the ArsA protein. J Biol Chem. 1990;265:190-4 pubmed
  7. Liu Z, Shen J, Carbrey J, Mukhopadhyay R, Agre P, Rosen B. Arsenite transport by mammalian aquaglyceroporins AQP7 and AQP9. Proc Natl Acad Sci U S A. 2002;99:6053-8 pubmed
  8. Lin Y, Yang J, Rosen B. ArsD: an As(III) metallochaperone for the ArsAB As(III)-translocating ATPase. J Bioenerg Biomembr. 2007;39:453-8 pubmed publisher
    ..It is proposed that ArsD residues Cys12, Cys13 and Cys18, but not Cys112, Cys113, Cys119 or Cys120, are required for delivery of As(III) to and activation of the ArsAB pump...
  9. Oden K, Gladysheva T, Rosen B. Arsenate reduction mediated by the plasmid-encoded ArsC protein is coupled to glutathione. Mol Microbiol. 1994;12:301-6 pubmed
    ..Since ArsC-catalysed reduction of arsenate provides high level resistance, physical coupling of the ArsC reaction to efflux of the resulting arsenite is hypothesised. ..

More Information

Publications17

  1. Shi J, Vlamis Gardikas A, Aslund F, Holmgren A, Rosen B. Reactivity of glutaredoxins 1, 2, and 3 from Escherichia coli shows that glutaredoxin 2 is the primary hydrogen donor to ArsC-catalyzed arsenate reduction. J Biol Chem. 1999;274:36039-42 pubmed
    ..This suggests that, during the catalytic cycle, ArsC forms a mixed disulfide with GSH before being reduced by glutaredoxin to regenerate the active ArsC reductase. ..
  2. Bhattacharjee H, Rosen B. Structure-function analysis of the ArsA ATPase: contribution of histidine residues. J Bioenerg Biomembr. 2001;33:459-68 pubmed
    ..The results indicate that no single histidine residue plays a direct role in catalysis, and the inhibition by DEPC may be caused by steric hindrance from the carbethoxy group. ..
  3. Fu H, Rosen B, Bhattacharjee H. Biochemical characterization of a novel ArsA ATPase complex from Alkaliphilus metalliredigens QYMF. FEBS Lett. 2010;584:3089-94 pubmed publisher
    ..The results show that AmArsA1-AmArsA2 interaction is needed to form the functional ArsA ATPase. This novel AmArsA1-AmArsA2 complex may provide insight in how it participates with Acr3 in arsenite detoxification...
  4. Fu H, Meng Y, Ord ez E, Villadangos A, Bhattacharjee H, Gil J, et al. Properties of arsenite efflux permeases (Acr3) from Alkaliphilus metalliredigens and Corynebacterium glutamicum. J Biol Chem. 2009;284:19887-95 pubmed publisher
    ..A scanning cysteine accessibility method was used to show that Acr3 has 10 transmembrane segments, and the conserved cysteine would be predicted to be in the fourth transmembrane segment...
  5. Qin J, Lehr C, Yuan C, Le X, McDermott T, Rosen B. Biotransformation of arsenic by a Yellowstone thermoacidophilic eukaryotic alga. Proc Natl Acad Sci U S A. 2009;106:5213-7 pubmed publisher
  6. Ruan X, Bhattacharjee H, Rosen B. Cys-113 and Cys-422 form a high affinity metalloid binding site in the ArsA ATPase. J Biol Chem. 2006;281:9925-34 pubmed
  7. Shen J, Hsu C, Kang B, Rosen B, Bhattacharjee H. The Saccharomyces cerevisiae Arr4p is involved in metal and heat tolerance. Biometals. 2003;16:369-78 pubmed
    ..It is proposed that under heat or metal stress, the soluble ATPase becomes membrane-associated, perhaps through interaction with a partner protein, and that this complex is involved in stress tolerance. ..
  8. Martin P, DeMel S, Shi J, Gladysheva T, Gatti D, Rosen B, et al. Insights into the structure, solvation, and mechanism of ArsC arsenate reductase, a novel arsenic detoxification enzyme. Structure. 2001;9:1071-81 pubmed