Experts and Doctors on arsenates in Detroit, Michigan, United States
Locale: Detroit, Michigan, United States
Carlin A, Shi W, Dey S, Rosen B. The ars operon of Escherichia coli confers arsenical and antimonial resistance. J Bacteriol. 1995;177:981-6 pubmed
..Expression of the ars genes was inducible by arsenite. By Southern hybridization, the operon was found in all strains of E. coli examined but not in Salmonella typhimurium, Pseudomonas aeruginosa, or Bacillus subtilis. ..
Rosen B, Weigel U, Monticello R, Edwards B. Molecular analysis of an anion pump: purification of the ArsC protein. Arch Biochem Biophys. 1991;284:381-5 pubmed
..The arsC gene was cloned behind a strong promoter and expressed at high levels. The ArsC protein was purified and crystallized. ..
Shi J, Vlamis Gardikas A, Aslund F, Holmgren A, Rosen B. Reactivity of glutaredoxins 1, 2, and 3 from Escherichia coli shows that glutaredoxin 2 is the primary hydrogen donor to ArsC-catalyzed arsenate reduction. J Biol Chem. 1999;274:36039-42 pubmed
..This suggests that, during the catalytic cycle, ArsC forms a mixed disulfide with GSH before being reduced by glutaredoxin to regenerate the active ArsC reductase. ..
Mukhopadhyay R, Shi J, Rosen B. Purification and characterization of ACR2p, the Saccharomyces cerevisiae arsenate reductase. J Biol Chem. 2000;275:21149-57 pubmed
..This suggests that during the catalytic cycle, Acr2p forms a mixed disulfide with GSH before being reduced by glutaredoxin to regenerate the active Acr2p reductase. ..
Shi J, Mukhopadhyay R, Rosen B. Identification of a triad of arginine residues in the active site of the ArsC arsenate reductase of plasmid R773. FEMS Microbiol Lett. 2003;227:295-301 pubmed
..The data support the hypothesis that this triad of arginine residues is involved in arsenate binding and transition-state stabilization. ..
Oden K, Gladysheva T, Rosen B. Arsenate reduction mediated by the plasmid-encoded ArsC protein is coupled to glutathione. Mol Microbiol. 1994;12:301-6 pubmed
..Since ArsC-catalysed reduction of arsenate provides high level resistance, physical coupling of the ArsC reaction to efflux of the resulting arsenite is hypothesised. ..
Martin P, DeMel S, Shi J, Gladysheva T, Gatti D, Rosen B, et al
. Insights into the structure, solvation, and mechanism of ArsC arsenate reductase, a novel arsenic detoxification enzyme. Structure. 2001;9:1071-81 pubmed
Mukhopadhyay R, Rosen B. Arsenate reductases in prokaryotes and eukaryotes. Environ Health Perspect. 2002;110 Suppl 5:745-8 pubmed
..The yeast Acr2p has an active site motif HC(X)(5)R that is conserved in protein phosphotyrosine phosphatases and rhodanases, suggesting that these three groups of enzymes may have evolved from an ancestral oxyanion-binding protein. ..
DeMel S, Shi J, Martin P, Rosen B, Edwards B. Arginine 60 in the ArsC arsenate reductase of E. coli plasmid R773 determines the chemical nature of the bound As(III) product. Protein Sci. 2004;13:2330-40 pubmed
..We propose that this intermediate is more stable than the monohydroxyarsenite intermediate of the native enzyme, resulting in slow release of product and, consequently, loss of activity. ..