Experts and Doctors on arabidopsis in East Lansing, Michigan, United States


Locale: East Lansing, Michigan, United States
Topic: arabidopsis

Top Publications

  1. McAndrew R, Olson B, Kadirjan Kalbach D, Chi Ham C, Vitha S, Froehlich J, et al. In vivo quantitative relationship between plastid division proteins FtsZ1 and FtsZ2 and identification of ARC6 and ARC3 in a native FtsZ complex. Biochem J. 2008;412:367-78 pubmed publisher
  2. Andre C, Froehlich J, Moll M, Benning C. A heteromeric plastidic pyruvate kinase complex involved in seed oil biosynthesis in Arabidopsis. Plant Cell. 2007;19:2006-22 pubmed
    ..Therefore, the identified pyruvate kinase catalyzes a crucial step in the conversion of photosynthate into oil, suggesting a preferred plastid route from its substrate phosphoenolpyruvate to fatty acids. ..
  3. Yang W, Pollard M, Li Beisson Y, Beisson F, Feig M, Ohlrogge J. A distinct type of glycerol-3-phosphate acyltransferase with sn-2 preference and phosphatase activity producing 2-monoacylglycerol. Proc Natl Acad Sci U S A. 2010;107:12040-5 pubmed publisher
    ..These results provide insight into the biosynthetic assembly of cutin and suberin, the two most abundant glycerolipid polymers in nature. ..
  4. Bassham D, Raikhel N. The pre-vacuolar t-SNARE AtPEP12p forms a 20S complex that dissociates in the presence of ATP. Plant J. 1999;19:599-603 pubmed
    ..These preliminary data suggest that AtPEP12p may function jointly with alpha-SNAP and NSF in the fusion of transport vesicles containing vacuolar cargo proteins with the pre-vacuolar compartment. ..
  5. Sattler S, Gilliland L, Magallanes Lundback M, Pollard M, DellaPenna D. Vitamin E is essential for seed longevity and for preventing lipid peroxidation during germination. Plant Cell. 2004;16:1419-32 pubmed
    ..The vte mutant phenotypes also explain the strong selection for retention of tocopherol biosynthesis during the evolution of seed-bearing plants. ..
  6. Tian L, DellaPenna D. Characterization of a second carotenoid beta-hydroxylase gene from Arabidopsis and its relationship to the LUT1 locus. Plant Mol Biol. 2001;47:379-88 pubmed
    ..These data indicate that the LUT1 locus encodes a protein necessary for epsilon-ring hydroxylation but unrelated to beta-hydroxylases at the level of amino acid sequence. ..
  7. Roston R, Gao J, Xu C, Benning C. Arabidopsis chloroplast lipid transport protein TGD2 disrupts membranes and is part of a large complex. Plant J. 2011;66:759-69 pubmed publisher
  8. Reumann S, Quan S, Aung K, Yang P, Manandhar Shrestha K, Holbrook D, et al. In-depth proteome analysis of Arabidopsis leaf peroxisomes combined with in vivo subcellular targeting verification indicates novel metabolic and regulatory functions of peroxisomes. Plant Physiol. 2009;150:125-43 pubmed publisher
    ..Thus, this study represents an important step toward defining the complete plant peroxisomal proteome. ..
  9. Cavalier D, Lerouxel O, Neumetzler L, Yamauchi K, Reinecke A, Freshour G, et al. Disrupting two Arabidopsis thaliana xylosyltransferase genes results in plants deficient in xyloglucan, a major primary cell wall component. Plant Cell. 2008;20:1519-37 pubmed publisher
    ..Moreover, the lack of detectable xyloglucan in the xxt1 xxt2 double mutant challenges conventional models of the plant primary cell wall. ..

More Information


  1. Maeda H, Sage T, Isaac G, Welti R, DellaPenna D. Tocopherols modulate extraplastidic polyunsaturated fatty acid metabolism in Arabidopsis at low temperature. Plant Cell. 2008;20:452-70 pubmed publisher
    ..These results provide biochemical and genetic evidence that plastid-synthesized tocopherols modulate ER PUFA metabolism early in the LT adaptation response of Arabidopsis. ..
  2. Gilliland L, Magallanes Lundback M, Hemming C, Supplee A, Koornneef M, Bentsink L, et al. Genetic basis for natural variation in seed vitamin E levels in Arabidopsis thaliana. Proc Natl Acad Sci U S A. 2006;103:18834-41 pubmed
  3. Moellering E, Muthan B, Benning C. Freezing tolerance in plants requires lipid remodeling at the outer chloroplast membrane. Science. 2010;330:226-8 pubmed publisher
    ..This SFR2-based mechanism compensates for changes in organelle volume and stabilizes membranes during freezing. ..
  4. Xu C, Yu B, Cornish A, Froehlich J, Benning C. Phosphatidylglycerol biosynthesis in chloroplasts of Arabidopsis mutants deficient in acyl-ACP glycerol-3- phosphate acyltransferase. Plant J. 2006;47:296-309 pubmed
    ..These observations indicate coordinated regulation of plastid lipid metabolism and plant development. ..
  5. Zheng Y, Anderson S, Zhang Y, Garavito R. The structure of sucrose synthase-1 from Arabidopsis thaliana and its functional implications. J Biol Chem. 2011;286:36108-18 pubmed publisher
    ..8- and 2.85-Å resolutions, respectively. The AtSus1 structure provides insights into sucrose catalysis and cleavage, as well as the regulation of sucrose synthase and its interactions with cellular targets. ..
  6. Stefano G, Renna L, Moss T, McNew J, Brandizzi F. In Arabidopsis, the spatial and dynamic organization of the endoplasmic reticulum and Golgi apparatus is influenced by the integrity of the C-terminal domain of RHD3, a non-essential GTPase. Plant J. 2012;69:957-66 pubmed publisher
    ..We conclude that RHD3 is important in ER morphology, but is dispensable for peripheral ER tubulation in an endogenous context, and that its activity relies on the C-terminal region in addition to the GTPase domain. ..
  7. Ahmed S, Rojo E, Kovaleva V, Venkataraman S, Dombrowski J, Matsuoka K, et al. The plant vacuolar sorting receptor AtELP is involved in transport of NH(2)-terminal propeptide-containing vacuolar proteins in Arabidopsis thaliana. J Cell Biol. 2000;149:1335-44 pubmed
    ..We hypothesize that AtELP functions as a vacuolar sorting receptor involved in the targeting of NTPP-, but not CTPP-containing proteins in Arabidopsis. ..
  8. Zheng H, von Mollard G, Kovaleva V, Stevens T, Raikhel N. The plant vesicle-associated SNARE AtVTI1a likely mediates vesicle transport from the trans-Golgi network to the prevacuolar compartment. Mol Biol Cell. 1999;10:2251-64 pubmed
    ..We propose that AtVTI1a functions as a v-SNARE responsible for targeting AtELP-containing vesicles from the trans-Golgi network to the PVC, and that AtVTI1b is involved in a different membrane transport process. ..
  9. Focks N, Sagasser M, Weisshaar B, Benning C. Characterization of tt15, a novel transparent testa mutant of Arabidopsis thaliana (L.) Heynh. Planta. 1999;208:352-7 pubmed
    ..Most notable was a reduction of the cyanidin and quercetin content of tt15 seeds. ..
  10. Bell S, Burgoon L, Last R. MIPHENO: data normalization for high throughput metabolite analysis. BMC Bioinformatics. 2012;13:10 pubmed publisher
    ..MIPHENO is applicable to a wide range of high throughput screenings and the code is freely available as Additional file 1 as well as through an R package in CRAN. ..
  11. Zou C, Lehti Shiu M, Thomashow M, Shiu S. Evolution of stress-regulated gene expression in duplicate genes of Arabidopsis thaliana. PLoS Genet. 2009;5:e1000581 pubmed publisher
  12. Kohzuma K, Dal Bosco C, Meurer J, Kramer D. Light- and metabolism-related regulation of the chloroplast ATP synthase has distinct mechanisms and functions. J Biol Chem. 2013;288:13156-63 pubmed publisher
  13. Bednarek S, Reynolds T, Schroeder M, Grabowski R, Hengst L, Gallwitz D, et al. A small GTP-binding protein from Arabidopsis thaliana functionally complements the yeast YPT6 null mutant. Plant Physiol. 1994;104:591-6 pubmed
    ..The isolation of this gene will aid in the dissection of the machinery involved in soluble protein sorting at the trans-Golgi network of plants. ..
  14. Taylor C, Bariola P, delCardayre S, Raines R, Green P. RNS2: a senescence-associated RNase of Arabidopsis that diverged from the S-RNases before speciation. Proc Natl Acad Sci U S A. 1993;90:5118-22 pubmed
    ..On the basis of these observations, we suggest that one role of RNS2 in Arabidopsis may be to remobilize phosphate, particularly when cells senesce or when phosphate becomes limiting. ..
  15. Gilmour S, Lin C, Thomashow M. Purification and properties of Arabidopsis thaliana COR (cold-regulated) gene polypeptides COR15am and COR6.6 expressed in Escherichia coli. Plant Physiol. 1996;111:293-9 pubmed
    ..Uemura, S.J. Gilmour, M.F. Thomashow, P.L. Steponkus [1996] Plant Physiology 111: 313-327), the effects of COR15amr and COR6.6r on the cryostability and lyotropic phase behavior of liposomes are examined. ..
  16. Withers J, Yao J, Mecey C, Howe G, Melotto M, He S. Transcription factor-dependent nuclear localization of a transcriptional repressor in jasmonate hormone signaling. Proc Natl Acad Sci U S A. 2012;109:20148-53 pubmed publisher
    ..Results also provide evidence for a transcription factor-dependent mechanism for nuclear import of a cognate transcriptional repressor JAZ9 in plants. ..
  17. Marti L, Stefano G, Tamura K, Hawes C, Renna L, Held M, et al. A missense mutation in the vacuolar protein GOLD36 causes organizational defects in the ER and aberrant protein trafficking in the plant secretory pathway. Plant J. 2010;63:901-13 pubmed publisher
    ..We speculate that GOLD36 may be a factor that is necessary for ER integrity because of its ability to limit deleterious effects of other secretory proteins on the ER. ..
  18. Schilmiller A, Koo A, Howe G. Functional diversification of acyl-coenzyme A oxidases in jasmonic acid biosynthesis and action. Plant Physiol. 2007;143:812-24 pubmed
    ..brassicicola challenge. Thus, different types of biotic stress may induce JA synthesis via distinct enzymatic routes. ..
  19. Nomura K, DebRoy S, Lee Y, Pumplin N, Jones J, He S. A bacterial virulence protein suppresses host innate immunity to cause plant disease. Science. 2006;313:220-3 pubmed
    ..HopM1 mediates the destruction of AtMIN7 via the host proteasome. Our results illustrate a strategy by which a bacterial pathogen exploits the host proteasome to subvert host immunity and causes infection in plants. ..
  20. Zhao Y, He S, Sundin G. The Erwinia amylovora avrRpt2EA gene contributes to virulence on pear and AvrRpt2EA is recognized by Arabidopsis RPS2 when expressed in pseudomonas syringae. Mol Plant Microbe Interact. 2006;19:644-54 pubmed
    ..The results also suggested that although distinct pathogens such as E. amylovora and P. syringae may contain similar effector genes, expression and secretion of these effectors can be under specific regulation by the native pathogen...
  21. Voll L, Jamai A, Renné P, Voll H, McClung C, Weber A. The photorespiratory Arabidopsis shm1 mutant is deficient in SHM1. Plant Physiol. 2006;140:59-66 pubmed
    ..Our findings establish SHM1 as the defective gene in the Arabidopsis shm1-1 mutant. ..
  22. Kosma D, Molina I, Ohlrogge J, Pollard M. Identification of an Arabidopsis fatty alcohol:caffeoyl-Coenzyme A acyltransferase required for the synthesis of alkyl hydroxycinnamates in root waxes. Plant Physiol. 2012;160:237-48 pubmed publisher
  23. Yang J, Han K. Functional characterization of allantoinase genes from Arabidopsis and a nonureide-type legume black locust. Plant Physiol. 2004;134:1039-49 pubmed
    ..In addition, the gene expression in the bark/cambial region was up-regulated in spring and fall when compared with summer, suggesting its involvement in nitrogen mobilization. ..
  24. Sanderfoot A, Kovaleva V, Bassham D, Raikhel N. Interactions between syntaxins identify at least five SNARE complexes within the Golgi/prevacuolar system of the Arabidopsis cell. Mol Biol Cell. 2001;12:3733-43 pubmed
    ..On the PVC, a SYP5-type syntaxin interacts specifically with a SYP2-type syntaxin, as well as the SNARE VTI11, forming a SNARE complex likely involved in TGN-to-PVC trafficking. ..
  25. Moellering E, Benning C. Phosphate regulation of lipid biosynthesis in Arabidopsis is independent of the mitochondrial outer membrane DGS1 complex. Plant Physiol. 2010;152:1951-9 pubmed publisher
    ..Apparently, the DGS1 wild-type protein does not directly affect lipid metabolism in mitochondria or chloroplasts. ..
  26. Glynn J, Yang Y, Vitha S, Schmitz A, Hemmes M, Miyagishima S, et al. PARC6, a novel chloroplast division factor, influences FtsZ assembly and is required for recruitment of PDV1 during chloroplast division in Arabidopsis. Plant J. 2009;59:700-11 pubmed publisher
    ..Our findings indicate that PARC6, like ARC6, plays a role in coordinating the internal and external components of the chloroplast division complex, but that PARC6 has evolved distinct functions in the division process. ..
  27. Ferguson A, Zhao D, Jiang N. Selective acquisition and retention of genomic sequences by Pack-Mutator-like elements based on guanine-cytosine content and the breadth of expression. Plant Physiol. 2013;163:1419-32 pubmed publisher
    ..Such selective acquisition/retention provides these elements greater chances of carrying functional sequences that may provide new genetic resources for the evolution of new genes or the modification of existing genes. ..
  28. Glynn J, Froehlich J, Osteryoung K. Arabidopsis ARC6 coordinates the division machineries of the inner and outer chloroplast membranes through interaction with PDV2 in the intermembrane space. Plant Cell. 2008;20:2460-70 pubmed publisher
  29. Tian M, Chaudhry F, Ruzicka D, Meagher R, Staiger C, Day B. Arabidopsis actin-depolymerizing factor AtADF4 mediates defense signal transduction triggered by the Pseudomonas syringae effector AvrPphB. Plant Physiol. 2009;150:815-24 pubmed publisher
    ..Collectively, this study identifies AtADF4 as a novel component of the plant defense signaling pathway and provides strong evidence for actin dynamics as a primary component that orchestrates plant defenses against P. syringae. ..
  30. Xu C, Fan J, Riekhof W, Froehlich J, Benning C. A permease-like protein involved in ER to thylakoid lipid transfer in Arabidopsis. EMBO J. 2003;22:2370-9 pubmed
    ..Mutations in a permease-like protein of the outer chloroplastic envelope are responsible for the primary biochemical defect. It is proposed that this protein is part of a lipid transfer complex. ..
  31. Stockinger E, Gilmour S, Thomashow M. Arabidopsis thaliana CBF1 encodes an AP2 domain-containing transcriptional activator that binds to the C-repeat/DRE, a cis-acting DNA regulatory element that stimulates transcription in response to low temperature and water deficit. Proc Natl Acad Sci U S A. 1997;94:1035-40 pubmed
    ..We conclude that CBF1 can function as a transcriptional activator that binds to the C-repeat/DRE DNA regulatory element and, thus, is likely to have a role in cold- and dehydration-regulated gene expression in Arabidopsis. ..
  32. Kaur N, Hu J. Dynamics of peroxisome abundance: a tale of division and proliferation. Curr Opin Plant Biol. 2009;12:781-8 pubmed publisher
    ..In this review we attempt to summarize the current state of our knowledge of peroxisome division/proliferation and their regulation in plants. ..
  33. Moreno J, Shyu C, Campos M, Patel L, Chung H, Yao J, et al. Negative feedback control of jasmonate signaling by an alternative splice variant of JAZ10. Plant Physiol. 2013;162:1006-17 pubmed publisher
    ..4 links transcription factors to a corepressor complex and suggest how JA-induced transcription and alternative splicing of JAZ10 premessenger RNA creates a regulatory circuit to attenuate JA responses. ..
  34. Beisson F, Li Y, Bonaventure G, Pollard M, Ohlrogge J. The acyltransferase GPAT5 is required for the synthesis of suberin in seed coat and root of Arabidopsis. Plant Cell. 2007;19:351-68 pubmed
    ..These results provide evidence for a critical role of GPAT5 in polyester biogenesis in seed coats and roots and for the importance of lipid polymer structures in the normal function of these organs. ..
  35. Cavalier D, Keegstra K. Two xyloglucan xylosyltransferases catalyze the addition of multiple xylosyl residues to cellohexaose. J Biol Chem. 2006;281:34197-207 pubmed
    ..On the basis of these results, we concluded that AtXT1 and AtXT2 have the same acceptor specificities and generate the same products in vitro. The implications of these results for understanding in vivo XyG biosynthesis are considered. ..
  36. Froehlich J, Benning C, Dormann P. The digalactosyldiacylglycerol (DGDG) synthase DGD1 is inserted into the outer envelope membrane of chloroplasts in a manner independent of the general import pathway and does not depend on direct interaction with monogalactosyldiacylglycerol synthas. J Biol Chem. 2001;276:31806-12 pubmed
    ..Transformation of DGD1 constructs into cyanobacteria resulted in the expression of active DGDG synthase and demonstrated that DGDG synthesis depends on MGDG lipid, but does not require direct interaction with the plant MGDG synthase. ..
  37. Gille S, Hänsel U, Ziemann M, Pauly M. Identification of plant cell wall mutants by means of a forward chemical genetic approach using hydrolases. Proc Natl Acad Sci U S A. 2009;106:14699-704 pubmed publisher
    ..Xeg-113 plants exhibit more elongated hypocotyls than WT, providing genetic evidence that plant O-glycosylation--more specifically, extensin arabinosylation--is important for cell elongation. ..
  38. Muthan B, Roston R, Froehlich J, Benning C. Probing Arabidopsis chloroplast diacylglycerol pools by selectively targeting bacterial diacylglycerol kinase to suborganellar membranes. Plant Physiol. 2013;163:61-74 pubmed publisher
    ..Finally, we use the outer envelope-targeted DAGK line as a tool to probe the accessibility of DAG generated in response to osmotic stress. ..
  39. Bariola P, Howard C, Taylor C, Verburg M, Jaglan V, Green P. The Arabidopsis ribonuclease gene RNS1 is tightly controlled in response to phosphate limitation. Plant J. 1994;6:673-85 pubmed
    ..The characterization of RNS1, in particular, provides an important first step towards elucidating the mechanisms by which plants sense and respond to P(i) limitation, a prominent condition in many soil types. ..
  40. Kadirjan Kalbach D, Yoder D, Ruckle M, Larkin R, Osteryoung K. FtsHi1/ARC1 is an essential gene in Arabidopsis that links chloroplast biogenesis and division. Plant J. 2012;72:856-67 pubmed publisher
    ..Taken together, our analyses reveal that FtsHi1 functions in an essential, envelope-associated process that may couple plastid development with division. ..
  41. Trentmann S, Kende H. Analysis of Arabidopsis cDNA that shows homology to the tomato E8 cDNA. Plant Mol Biol. 1995;29:161-6 pubmed
    ..The 2A6 protein shows three domains that are highly conserved among E8, ACC oxidases, and 2-ODDs. ..
  42. Sanjaya -, Miller R, Durrett T, Kosma D, Lydic T, Muthan B, et al. Altered lipid composition and enhanced nutritional value of Arabidopsis leaves following introduction of an algal diacylglycerol acyltransferase 2. Plant Cell. 2013;25:677-93 pubmed publisher
  43. Cocuron J, Lerouxel O, Drakakaki G, Alonso A, Liepman A, Keegstra K, et al. A gene from the cellulose synthase-like C family encodes a beta-1,4 glucan synthase. Proc Natl Acad Sci U S A. 2007;104:8550-5 pubmed
    ..These and other observations provide a compelling case that the CSLC gene family encode proteins that synthesize the XyG backbone...
  44. Li Y, Beisson F, Ohlrogge J, Pollard M. Monoacylglycerols are components of root waxes and can be produced in the aerial cuticle by ectopic expression of a suberin-associated acyltransferase. Plant Physiol. 2007;144:1267-77 pubmed
  45. Zeng W, Brutus A, Kremer J, Withers J, Gao X, Jones A, et al. A genetic screen reveals Arabidopsis stomatal and/or apoplastic defenses against Pseudomonas syringae pv. tomato DC3000. PLoS Pathog. 2011;7:e1002291 pubmed publisher
    ..Identification of SCORD5 begins to implicate an important role of stress-associated protein translation in stomatal guard cell signaling in response to microbe-associated molecular patterns and bacterial infection. ..
  46. Salas J, Ohlrogge J. Characterization of substrate specificity of plant FatA and FatB acyl-ACP thioesterases. Arch Biochem Biophys. 2002;403:25-34 pubmed
    ..The characterization of the resulting chimeric enzymes pointed to the N-terminus as a determinant of the substrate specificity for both FatA and FatB acyl-ACP thioesterases. ..
  47. Bonaventure G, Ohlrogge J. Differential regulation of mRNA levels of acyl carrier protein isoforms in Arabidopsis. Plant Physiol. 2002;128:223-35 pubmed
    ..The deletion of 58 bp containing the conserved motifs of the ACP1 5'-UTR resulted in 10- to 20-fold lower gene expression in leaf and seed tissues of transgenic Arabidopsis plants. ..
  48. Varbanova M, Porter K, Lu F, Ralph J, Hammerschmidt R, Jones A, et al. Molecular and biochemical basis for stress-induced accumulation of free and bound p-coumaraldehyde in cucumber. Plant Physiol. 2011;157:1056-66 pubmed publisher
    ..This mechanism represents a potentially evolutionarily conserved process to efficiently and quickly respond to biotic and abiotic stresses in cucurbit plants, resulting in the rapid lignification of affected tissues...
  49. Gilliland L, Pawloski L, Kandasamy M, Meagher R. Arabidopsis actin gene ACT7 plays an essential role in germination and root growth. Plant J. 2003;33:319-28 pubmed
    ..The act7 mutant phenotypes demonstrate a significant requirement for functional ACT7 protein during root development and explain the strong negative selection component seen for the act7-1 mutant. ..
  50. Conger R, Chen Y, Fornaciari S, Faso C, Held M, Renna L, et al. Evidence for the involvement of the Arabidopsis SEC24A in male transmission. J Exp Bot. 2011;62:4917-26 pubmed publisher
    ..The results also attribute a novel role to SEC24 proteins in a multicellular model system, specifically in male fertility. ..
  51. Pan R, Hu J. The conserved fission complex on peroxisomes and mitochondria. Plant Signal Behav. 2011;6:870-2 pubmed
    ..Sharing a conserved fission machine between these metabolically linked subcellular compartments throughout evolution may have some biological significance. ..
  52. Adhikari N, Froehlich J, Strand D, Buck S, Kramer D, Larkin R. GUN4-porphyrin complexes bind the ChlH/GUN5 subunit of Mg-Chelatase and promote chlorophyll biosynthesis in Arabidopsis. Plant Cell. 2011;23:1449-67 pubmed publisher
  53. Zeng W, Keegstra K. AtCSLD2 is an integral Golgi membrane protein with its N-terminus facing the cytosol. Planta. 2008;228:823-38 pubmed publisher
    ..Combining this observation with the transmembrane domain predictions, we postulate that the large hydrophilic domain of this protein also faces the cytosol. ..