Experts and Doctors on affinity chromatography in Ann Arbor, Michigan, United States


Locale: Ann Arbor, Michigan, United States
Topic: affinity chromatography

Top Publications

  1. Korber P, Zander T, Herschlag D, Bardwell J. A new heat shock protein that binds nucleic acids. J Biol Chem. 1999;274:249-56 pubmed
    ..0, 5 mM MgCl2. A role of Hsp15 in binding nucleic acids puts this protein into a different functional category from that of many other heat shock proteins that act as molecular chaperones or proteases on protein substrates. ..
  2. Das A, Uhler M, Hajra A. Molecular cloning and expression of mammalian peroxisomal trans-2-enoyl-coenzyme A reductase cDNAs. J Biol Chem. 2000;275:24333-40 pubmed publisher
    ..Northern blot analysis demonstrated that a single transcript of 1.3 kilobases is present in most mouse tissues, with particularly high concentrations in liver and kidney...
  3. O Rourke K, Laherty C, Dixit V. Thrombospondin 1 and thrombospondin 2 are expressed as both homo- and heterotrimers. J Biol Chem. 1992;267:24921-4 pubmed
    ..In addition, homotrimeric TSP2 has a lower affinity for heparin than homotrimeric TSP1. Thus, the heparin affinity of TSP can be modulated by the expression of TSP as homo- or heterotrimers. ..
  4. Larsen R, Rajan V, Ruff M, Kukowska Latallo J, Cummings R, Lowe J. Isolation of a cDNA encoding a murine UDPgalactose:beta-D-galactosyl- 1,4-N-acetyl-D-glucosaminide alpha-1,3-galactosyltransferase: expression cloning by gene transfer. Proc Natl Acad Sci U S A. 1989;86:8227-31 pubmed
    ..This cloning approach may be generally applicable to the isolation of cDNAs encoding other mammalian glycosyltransferases. ..
  5. Shibuya N, Goldstein I, Van Damme E, Peumans W. Binding properties of a mannose-specific lectin from the snowdrop (Galanthus nivalis) bulb. J Biol Chem. 1988;263:728-34 pubmed
    ..Glycopeptides which carry Man(alpha 1-3)Man units were retarded on the immobilized GNA column whereas those lacking this unit or with hybrid type glycan chains were not retarded on the column...
  6. Fu G, Markovitz D. Purification of the pets factor. A nuclear protein that binds to the inducible TG-rich element of the human immunodeficiency virus type 2 enhancer. J Biol Chem. 1996;271:19599-605 pubmed
    ..As retroviruses generally expropriate important human regulatory proteins for their own use, the 43-kDa pets factor is also likely to play a significant role in signal transduction in T cells and in other cellular processes. ..
  7. Tayeh M, Dotson G, Clemens J, Woodard R. Overproduction and one-step purification of Escherichia coli UDP-N-acetylglucosamine enolpyruvyl reductase. Protein Expr Purif. 1995;6:757-62 pubmed
  8. Sheppard C, Trimmer E, Matthews R. Purification and properties of NADH-dependent 5, 10-methylenetetrahydrofolate reductase (MetF) from Escherichia coli. J Bacteriol. 1999;181:718-25 pubmed
  9. Heaton J, Dlakic W, Dlakic M, Gelehrter T. Identification and cDNA cloning of a novel RNA-binding protein that interacts with the cyclic nucleotide-responsive sequence in the Type-1 plasminogen activator inhibitor mRNA. J Biol Chem. 2001;276:3341-7 pubmed
    ..Thus, we have described a novel RNA-binding protein that identifies a family of proteins with a previously undefined sequence motif. Our results suggest that this protein, PAI-RBP1, may play a role in regulation of mRNA stability. ..

More Information


  1. SpychaƂa J, Madrid Marina V, Fox I. High Km soluble 5'-nucleotidase from human placenta. Properties and allosteric regulation by IMP and ATP. J Biol Chem. 1988;263:18759-65 pubmed
  2. Czar M, Owens Grillo J, Dittmar K, Hutchison K, Zacharek A, Leach K, et al. Characterization of the protein-protein interactions determining the heat shock protein (hsp90.hsp70.hsp56) heterocomplex. J Biol Chem. 1994;269:11155-61 pubmed
    ..The presence of hsp56 in the heat shock protein heterocomplex is not stabilized by molybdate but hsp56 is stabilized if the glucocorticoid receptor is present in addition to hsp90 and hsp70. ..
  3. Chen Y, Kelly V, Stachura S, Garcia G. Characterization of the human tRNA-guanine transglycosylase: confirmation of the heterodimeric subunit structure. RNA. 2010;16:958-68 pubmed publisher
    ..hQTRTD1 has been implicated as the salvage enzyme that generates free queuine from QMP. Work is ongoing in our laboratory to confirm this activity. ..
  4. Winter H, Goldstein I. Facile preparation of the alpha-Gal-recognizing Griffonia simplicifolia I-B4 isolectin. Carbohydr Res. 2004;339:153-5 pubmed
    ..The procedure has the advantages over previous affinity procedures of speed (the isolation of B4 isolectin can be achieved in one day), simplicity, and high degree of resolution of the B4 isolectin...