Genomes and Genes
Experts and Doctors on saccharomyces cerevisiae proteins in Boston, Massachusetts, United States
Locale: Boston, Massachusetts, United States
Topic: saccharomyces cerevisiae proteins
Publications192 found, 100 shown here
- Maeda T, Tsai A, Saito H. Mutations in a protein tyrosine phosphatase gene (PTP2) and a protein serine/threonine phosphatase gene (PTC1) cause a synthetic growth defect in Saccharomyces cerevisiae. Mol Cell Biol. 1993;13:5408-17 pubmed..GST-PTC1 also had weak (approximately 0.5% of its serine phosphatase activity) protein tyrosine phosphatase activity. ..
- Marsischky G, Lee S, Griffith J, Kolodner R. 'Saccharomyces cerevisiae MSH2/6 complex interacts with Holliday junctions and facilitates their cleavage by phage resolution enzymes. J Biol Chem. 1999;274:7200-6 pubmed..This is consistent with the view that the MSH2/6 complex can function in both mismatch repair and the resolution of recombination intermediates as predicted by genetic studies. ..
- Marsischky G, Kolodner R. Biochemical characterization of the interaction between the Saccharomyces cerevisiae MSH2-MSH6 complex and mispaired bases in DNA. J Biol Chem. 1999;274:26668-82 pubmed
- Shim E, Walker A, Shi Y, Blackwell T. CDK-9/cyclin T (P-TEFb) is required in two postinitiation pathways for transcription in the C. elegans embryo. Genes Dev. 2002;16:2135-46 pubmed
- Storchova Z, Breneman A, Cande J, Dunn J, Burbank K, O TOOLE E, et al. Genome-wide genetic analysis of polyploidy in yeast. Nature. 2006;443:541-7 pubmed..Thus, geometric constraints may have profound effects on genome stability; the phenomenon described here may be relevant in a variety of biological contexts, including disease states such as cancer. ..
- Vasiljeva L, Kim M, Mutschler H, Buratowski S, Meinhart A. The Nrd1-Nab3-Sen1 termination complex interacts with the Ser5-phosphorylated RNA polymerase II C-terminal domain. Nat Struct Mol Biol. 2008;15:795-804 pubmed publisher..Nrd1 recruitment to genes involves a combination of interactions with CTD and Nab3. ..
- Kadowaki T, Schneiter R, Hitomi M, Tartakoff A. Mutations in nucleolar proteins lead to nucleolar accumulation of polyA+ RNA in Saccharomyces cerevisiae. Mol Biol Cell. 1995;6:1103-10 pubmed..mRNA may normally encounter nucleolar components before export and proteins such as Mtr3p may be critical for export of both mRNA and ribosomal subunits. ..
- Gozani O, Feld R, Reed R. Evidence that sequence-independent binding of highly conserved U2 snRNP proteins upstream of the branch site is required for assembly of spliceosomal complex A. Genes Dev. 1996;10:233-43 pubmed..These data indicate that sequence-independent binding of the highly conserved SF3a/SF3b subunits upstream of the branch site is essential for anchoring U2 snRNP to pre-mRNA. ..
- Laprade L, Boyartchuk V, Dietrich W, Winston F. Spt3 plays opposite roles in filamentous growth in Saccharomyces cerevisiae and Candida albicans and is required for C. albicans virulence. Genetics. 2002;161:509-19 pubmed..Furthermore, C. albicans spt3Delta/spt3Delta mutants are avirulent in mice. These experiments demonstrate that Spt3 plays important but opposite roles in filamentous growth in S. cerevisiae and C. albicans. ..
- Lei E, Stern C, Fahrenkrog B, Krebber H, Moy T, Aebi U, et al. Sac3 is an mRNA export factor that localizes to cytoplasmic fibrils of nuclear pore complex. Mol Biol Cell. 2003;14:836-47 pubmed..Finally, Mex67 accumulates at the nuclear rim when SAC3 is mutated, suggesting that Sac3 functions in Mex67 translocation through the NPC. ..
- Walker A, Shi Y, Blackwell T. An extensive requirement for transcription factor IID-specific TAF-1 in Caenorhabditis elegans embryonic transcription. J Biol Chem. 2004;279:15339-47 pubmed..Our findings imply that in metazoans TFIID may be of widespread importance for transcription and for expression of tissue-specific genes. ..
- Flotho A, Simpson D, Qi M, Elion E. Localized feedback phosphorylation of Ste5p scaffold by associated MAPK cascade. J Biol Chem. 2004;279:47391-401 pubmed..These findings provide evidence of a spatially regulated mechanism for post-activation control of a signaling scaffold that potentiates pathway activation. ..
- Wade J, Hall D, Struhl K. The transcription factor Ifh1 is a key regulator of yeast ribosomal protein genes. Nature. 2004;432:1054-8 pubmed..Thus, Ifh1 association with promoters is the key regulatory step for coordinate expression of RP genes. ..
- Hess D, Winston F. Evidence that Spt10 and Spt21 of Saccharomyces cerevisiae play distinct roles in vivo and functionally interact with MCB-binding factor, SCB-binding factor and Snf1. Genetics. 2005;170:87-94 pubmed..Taken together, these genetic interactions suggest distinct roles for Spt21 and Spt10 in vivo that are sensitive to multiple perturbations in transcription networks. ..
- Proft M, Mas G, de Nadal E, Vendrell A, Noriega N, Struhl K, et al. The stress-activated Hog1 kinase is a selective transcriptional elongation factor for genes responding to osmotic stress. Mol Cell. 2006;23:241-50 pubmed..Thus, in addition to its various functions during transcriptional initiation, Hog1 behaves as a transcriptional elongation factor that is selective for genes induced upon osmotic stress. ..
- Monahan B, Villen J, Marguerat S, Bahler J, Gygi S, Winston F. Fission yeast SWI/SNF and RSC complexes show compositional and functional differences from budding yeast. Nat Struct Mol Biol. 2008;15:873-80 pubmed publisher..Finally, phenotypic and microarray analyses identified widespread requirements for SWI/SNF and RSC on transcription including strong evidence that SWI/SNF directly represses iron-transport genes. ..
- Eisenmann D, Arndt K, Ricupero S, Rooney J, Winston F. SPT3 interacts with TFIID to allow normal transcription in Saccharomyces cerevisiae. Genes Dev. 1992;6:1319-31 pubmed..Taken together, these results suggest that SPT3 is a TFIID-associated protein, required for TFIID to function at particular promoters in vivo. ..
- Wei Y, Chen B, Samson L. Suppression of Escherichia coli alkB mutants by Saccharomyces cerevisiae genes. J Bacteriol. 1995;177:5009-15 pubmed..coli via an alkB-like mechanism remains to be determined, but protection appears to be specific for AlkB-deficient E. coli because none of the genes protect other alkylation-sensitive E. coli strains from killing by MMS. ..
- Bastos R, Ribas de Pouplana L, Enarson M, Bodoor K, Burke B. Nup84, a novel nucleoporin that is associated with CAN/Nup214 on the cytoplasmic face of the nuclear pore complex. J Cell Biol. 1997;137:989-1000 pubmed..In this way, Nup84 could play a central role in the organization of the interface between the pore complex and the cytoplasm. ..
- Hanna Rose W, Licht J, Hansen U. Two evolutionarily conserved repression domains in the Drosophila Kruppel protein differ in activator specificity. Mol Cell Biol. 1997;17:4820-9 pubmed..The different activator specificities of the two regions suggest that they repress transcription by different mechanisms and may play distinct biological roles during Drosophila development. ..
- Wang C, Chua K, Seghezzi W, Lees E, Gozani O, Reed R. Phosphorylation of spliceosomal protein SAP 155 coupled with splicing catalysis. Genes Dev. 1998;12:1409-14 pubmed..Significantly, SAP 155 is phosphorylated concomitant with or just after catalytic step one, making this the first example of a protein modification tightly regulated with splicing catalysis. ..
- Du K, Montminy M. CREB is a regulatory target for the protein kinase Akt/PKB. J Biol Chem. 1998;273:32377-9 pubmed..Our results support the notion that Akt/PKB promotes cell survival, at least in part, by stimulating the expression of cellular genes via the CREB/CBP nuclear transduction pathway. ..
- Schwabish M, Struhl K. Asf1 mediates histone eviction and deposition during elongation by RNA polymerase II. Mol Cell. 2006;22:415-22 pubmed..Lastly, Asf1 inhibits internal initiation from cryptic promoters within coding regions. These results strongly suggest that Asf1 functions as an elongation factor to disassemble and reassemble histones during Pol II elongation. ..
- Kahana J, Schnapp B, Silver P. Kinetics of spindle pole body separation in budding yeast. Proc Natl Acad Sci U S A. 1995;92:9707-11 pubmed..These results indicate that distinct forces act upon the spindle at different times during anaphase. ..
- Fernandes L, Rodrigues Pousada C, Struhl K. Yap, a novel family of eight bZIP proteins in Saccharomyces cerevisiae with distinct biological functions. Mol Cell Biol. 1997;17:6982-93 pubmed..Thus, members of the Yap family carry out overlapping but distinct biological functions. ..
- Wang Y, Elion E. Nuclear export and plasma membrane recruitment of the Ste5 scaffold are coordinated with oligomerization and association with signal transduction components. Mol Biol Cell. 2003;14:2543-58 pubmed
- Wu P, Ruhlmann C, Winston F, Schultz P. Molecular architecture of the S. cerevisiae SAGA complex. Mol Cell. 2004;15:199-208 pubmed..Our data provide insights into the molecular architecture of SAGA and imply a functional organization to the complex. ..
- DERMODY J, Buratowski S. Leo1 subunit of the yeast paf1 complex binds RNA and contributes to complex recruitment. J Biol Chem. 2010;285:33671-9 pubmed publisher..Together, these results suggest that association of Paf1C with RNA stabilizes its localization at actively transcribed regions where it influences chromatin structure. ..
- Park S, Li X, Kim H, Singh C, Tian G, Hoyt M, et al. Reconfiguration of the proteasome during chaperone-mediated assembly. Nature. 2013;497:512-6 pubmed publisher..Thus, the Rpt-CP interface is reconfigured when the lid complex joins the nascent proteasome to form the mature holoenzyme. ..
- Swanson M, Carlson M, Winston F. SPT6, an essential gene that affects transcription in Saccharomyces cerevisiae, encodes a nuclear protein with an extremely acidic amino terminus. Mol Cell Biol. 1990;10:4935-41 pubmed..By use of an epitope-tagged SPT6 protein, we have determined by indirect immunofluorescence that the SPT6 protein is located in the nucleus. ..
- Dollard C, Ricupero Hovasse S, Natsoulis G, Boeke J, Winston F. SPT10 and SPT21 are required for transcription of particular histone genes in Saccharomyces cerevisiae. Mol Cell Biol. 1994;14:5223-8 pubmed..These results suggest that under some conditions, S. cerevisiae may control the level of histone proteins by differential expression of its histone genes. ..
- Elion E, Satterberg B, Kranz J. FUS3 phosphorylates multiple components of the mating signal transduction cascade: evidence for STE12 and FAR1. Mol Biol Cell. 1993;4:495-510 pubmed..These data support a model in which FUS3 mediates transcription and G1 arrest by direct activation of STE12 and FAR1 and phosphorylates many other proteins involved in the response to pheromone. ..
- Garcia Rodriguez C, Rao A. Nuclear factor of activated T cells (NFAT)-dependent transactivation regulated by the coactivators p300/CREB-binding protein (CBP). J Exp Med. 1998;187:2031-6 pubmed..Recruitment of the coactivators p300/CBP by the transactivation domains of NFAT proteins is likely to play a critical role in NFAT-dependent gene expression during the immune response. ..
- Michel B, Komarnitsky P, Buratowski S. Histone-like TAFs are essential for transcription in vivo. Mol Cell. 1998;2:663-73 pubmed..Therefore, in contrast to previous studies in yeast that found only limited roles for TAFs in transcription, we find that the histone-like TAFs are generally required for in vivo transcription. ..
- Komarnitsky P, Michel B, Buratowski S. TFIID-specific yeast TAF40 is essential for the majority of RNA polymerase II-mediated transcription in vivo. Genes Dev. 1999;13:2484-9 pubmed..Here we show that mutations in the yeast TFIID-specific protein Taf40 lead to a general cessation of transcription, even in the presence of excess TBP, suggesting that the TFIID complex is required at most promoters in vivo. ..
- See R, Calvo D, Kawa H, Luke M, Yuan Z, Shi Y. Stimulation of p300-mediated transcription by the kinase MEKK1. J Biol Chem. 2001;276:16310-7 pubmed..Taken together, these results identify MEKK1 as a kinase that is likely to be involved in the regulation of the transactivation potential of p300 and support a role of p300 in MEKK1-induced apoptosis. ..
- Ye Y, Meyer H, Rapoport T. The AAA ATPase Cdc48/p97 and its partners transport proteins from the ER into the cytosol. Nature. 2001;414:652-6 pubmed..We propose that the Cdc48/p97-Ufd1-Npl4 complex extracts proteins from the ER membrane for cytosolic degradation. ..
- Eroglu B, Powers Lee S. Unmasking a functional allosteric domain in an allosterically nonresponsive carbamoyl-phosphate synthetase. J Biol Chem. 2002;277:45466-72 pubmed..These findings strongly suggest that the nonresponsive sCPS is not able to communicate occupancy of the allosteric site to the active site but does contain a latent allosteric interaction domain. ..
- Hall D, Struhl K. The VP16 activation domain interacts with multiple transcriptional components as determined by protein-protein cross-linking in vivo. J Biol Chem. 2002;277:46043-50 pubmed..We show that the VP16 activation domain directly interacts with TATA-binding protein (TBP), TFIIB, and the SAGA histone acetylase complex in vivo. ..
- Elsasser S, Chandler Militello D, Müller B, Hanna J, Finley D. Rad23 and Rpn10 serve as alternative ubiquitin receptors for the proteasome. J Biol Chem. 2004;279:26817-22 pubmed..Surprisingly, Rad23 and Ubp6 do not compete with each other for proteasome binding. Thus, Rpn1 may act as a scaffold to assemble on the proteasome multiple proteins that act to either bind or hydrolyze multiubiquitin chains. ..
- Pan F, Egile C, Lipkin T, Li R. ARPC1/Arc40 mediates the interaction of the actin-related protein 2 and 3 complex with Wiskott-Aldrich syndrome protein family activators. J Biol Chem. 2004;279:54629-36 pubmed
- Hieronymus H, Yu M, Silver P. Genome-wide mRNA surveillance is coupled to mRNA export. Genes Dev. 2004;18:2652-62 pubmed..These results demonstrate coupling of mRNA surveillance to mRNA export and suggest specificity of the RNA surveillance machinery for different transcript populations. Broadly, these findings link DNA and RNA surveillance to mRNA export. ..
- Markstein M, Pitsouli C, Villalta C, Celniker S, Perrimon N. Exploiting position effects and the gypsy retrovirus insulator to engineer precisely expressed transgenes. Nat Genet. 2008;40:476-83 pubmed publisher..The generality of our approach makes it adaptable to other model systems to identify and modify loci for optimal transgene expression. ..
- DeLuna A, Springer M, Kirschner M, Kishony R. Need-based up-regulation of protein levels in response to deletion of their duplicate genes. PLoS Biol. 2010;8:e1000347 pubmed publisher..Physiologically, such need-based responsiveness could provide an adaptive mechanism for compensation of genetic, environmental, or stochastic perturbations in protein abundance. ..
- Ezeokonkwo C, Zhelkovsky A, Lee R, Bohm A, Moore C. A flexible linker region in Fip1 is needed for efficient mRNA polyadenylation. RNA. 2011;17:652-64 pubmed publisher..Our results indicate that the Fip1 linker, through its flexibility and protein/protein interactions, allows Pap1 to reach the 3' end of the cleaved RNA and efficiently initiate poly(A) addition. ..
- Bergwitz C, Rasmussen M, Derobertis C, Wee M, Sinha S, Chen H, et al. Roles of major facilitator superfamily transporters in phosphate response in Drosophila. PLoS ONE. 2012;7:e31730 pubmed publisher..Finally, while phosphate is essential for Drosophila larval development, loss of MFS13 activity is compatible with viability indicating redundancy at the levels of the transporters...
- Hanna J, Waterman D, Boselli M, Finley D. Spg5 protein regulates the proteasome in quiescence. J Biol Chem. 2012;287:34400-9 pubmed publisher..Thus, Spg5 is a positive regulator of the proteasome that is critical for survival of cells that have ceased to proliferate due to nutrient limitation. ..
- Kuras L, Struhl K. Binding of TBP to promoters in vivo is stimulated by activators and requires Pol II holoenzyme. Nature. 1999;399:609-13 pubmed
- Helmlinger D, Marguerat S, Villen J, Gygi S, Bahler J, Winston F. The S. pombe SAGA complex controls the switch from proliferation to sexual differentiation through the opposing roles of its subunits Gcn5 and Spt8. Genes Dev. 2008;22:3184-95 pubmed publisher..Thus, SAGA plays distinct roles in the control of the switch from proliferation to differentiation in S. pombe through the dynamic and opposing activities of Gcn5 and Spt8. ..
- Meyers R, Cantley L. Cloning and characterization of a wortmannin-sensitive human phosphatidylinositol 4-kinase. J Biol Chem. 1997;272:4384-90 pubmed..Catt, J. K., and Balla, T. (1995) Proc. Natl. Acad. Sci. U. S. A. 92, 5317-5321). ..
- Larschan E, Winston F. The S. cerevisiae SAGA complex functions in vivo as a coactivator for transcriptional activation by Gal4. Genes Dev. 2001;15:1946-56 pubmed..These results, taken together with previous studies, demonstrate a dependent pathway for the recruitment of TBP to GAL gene promoters consisting of the recruitment of SAGA by Gal4 and the subsequent recruitment of TBP by SAGA. ..
- Raschle M, Van Komen S, Chi P, Ellenberger T, Sung P. Multiple interactions with the Rad51 recombinase govern the homologous recombination function of Rad54. J Biol Chem. 2004;279:51973-80 pubmed..These results highlight the functional importance of the N-terminal Rad51 interaction domain of Rad54 and reveal that Rad54 contacts Rad51 through separable epitopes. ..
- Mattheakis L, Shen W, Collier R. DPH5, a methyltransferase gene required for diphthamide biosynthesis in Saccharomyces cerevisiae. Mol Cell Biol. 1992;12:4026-37 pubmed..Consistent with these results, elongation factor 2 from the dph5 null mutant was found to have weak ADP-ribosyl acceptor activity, which was detectable only in the presence of high concentrations of fragment A. ..
- Lee H, Chun M, Kandror K. Tip60 and HDAC7 interact with the endothelin receptor a and may be involved in downstream signaling. J Biol Chem. 2001;276:16597-600 pubmed..We thus suggest that protein acetylase and deacetylase interact with ETA in a ligand-dependent fashion and may participate in ET signal transduction. ..
- Anderson R, Bitterman K, Wood J, Medvedik O, Sinclair D. Nicotinamide and PNC1 govern lifespan extension by calorie restriction in Saccharomyces cerevisiae. Nature. 2003;423:181-5 pubmed..We conclude that yeast lifespan extension by calorie restriction is the consequence of an active cellular response to a low-intensity stress and speculate that nicotinamide might regulate critical cellular processes in higher organisms. ..
- Zhu C, Byers K, McCord R, Shi Z, Berger M, Newburger D, et al. High-resolution DNA-binding specificity analysis of yeast transcription factors. Genome Res. 2009;19:556-66 pubmed publisher..These approaches could be adapted to identify TFs and cis regulatory elements in higher eukaryotes. ..
- Petrini J, Walsh M, DiMare C, Chen X, Korenberg J, Weaver D. Isolation and characterization of the human MRE11 homologue. Genomics. 1995;29:80-6 pubmed..The MRE11 locus maps to human chromosome 11q21 in a region frequently associated with cancer-related chromosomal abnormalities. A MRE11-related locus was found on chromosome 7q11.2-q11.3. ..
- Kessler M, Zhao J, Moore C. Purification of the Saccharomyces cerevisiae cleavage/polyadenylation factor I. Separation into two components that are required for both cleavage and polyadenylation of mRNA 3' ends. J Biol Chem. 1996;271:27167-75 pubmed..RNA15 is the only subunit of CF I that can be cross-linked to pre-mRNA. ..
- Halfon M, Gisselbrecht S, Lu J, Estrada B, Keshishian H, Michelson A. New fluorescent protein reporters for use with the Drosophila Gal4 expression system and for vital detection of balancer chromosomes. Genesis. 2002;34:135-8 pubmed
- de Carvalho C, COLAIACOVO M. SUMO-mediated regulation of synaptonemal complex formation during meiosis. Genes Dev. 2006;20:1986-92 pubmed
- Duina A, Rufiange A, Bracey J, Hall J, Nourani A, Winston F. Evidence that the localization of the elongation factor Spt16 across transcribed genes is dependent upon histone H3 integrity in Saccharomyces cerevisiae. Genetics. 2007;177:101-12 pubmed
- Hughes R, Chan F, White R, Zon L. Cloning and chromosomal localization of a mouse cDNA with homology to the Saccharomyces cerevisiae gene zuotin. Genomics. 1995;29:546-50 pubmed..The DnaJ domain and candidate phosphorylation sites of zuotin and ZRF1 are highly conserved. ZRF1 gene is localized on chromosome 5. The structural similarity of zuotin and ZRF1 suggests conservation of function of this DnaJ subfamily. ..
- Feng Y, Song L, Kincaid E, Mahanty S, Elion E. Functional binding between Gbeta and the LIM domain of Ste5 is required to activate the MEKK Ste11. Curr Biol. 1998;8:267-78 pubmed..Pheromone also induces Ste5-dependent phosphorylation of Gbeta. ..
- Matangkasombut O, Buratowski R, Swilling N, Buratowski S. Bromodomain factor 1 corresponds to a missing piece of yeast TFIID. Genes Dev. 2000;14:951-62 pubmed..The structural and functional similarities suggest that Bdf1 corresponds to the carboxy-terminal region of higher eukaryotic TAF(II)250 and that the interaction between TFIID and Bdf1 is important for proper gene expression. ..
- Geisberg J, Holstege F, Young R, Struhl K. Yeast NC2 associates with the RNA polymerase II preinitiation complex and selectively affects transcription in vivo. Mol Cell Biol. 2001;21:2736-42 pubmed..Thus, NC2 is associated with the Pol II preinitiation complex, and it can play a direct and positive role at certain promoters in vivo. ..
- Peng J, Schwartz D, Elias J, Thoreen C, Cheng D, Marsischky G, et al. A proteomics approach to understanding protein ubiquitination. Nat Biotechnol. 2003;21:921-6 pubmed..The methodology described here provides a general tool for the large-scale analysis and characterization of protein ubiquitination. ..
- Shaw G, Cope J, Li L, Corson K, Hersey C, Ackermann G, et al. Mitoferrin is essential for erythroid iron assimilation. Nature. 2006;440:96-100 pubmed..Our data show that mfrn functions as the principal mitochondrial iron importer essential for haem biosynthesis in vertebrate erythroblasts...
- Helmling S, Zhelkovsky A, Moore C. Fip1 regulates the activity of Poly(A) polymerase through multiple interactions. Mol Cell Biol. 2001;21:2026-37 pubmed..These findings show that Fip1 has a crucial regulatory function in the polyadenylation reaction by controlling the activity of poly(A) tail synthesis through multiple interactions within the polyadenylation complex. ..
- Arad M, Benson D, Perez Atayde A, McKenna W, Sparks E, Kanter R, et al. Constitutively active AMP kinase mutations cause glycogen storage disease mimicking hypertrophic cardiomyopathy. J Clin Invest. 2002;109:357-62 pubmed
- Arata Y, Baleja J, Forgac M. Localization of subunits D, E, and G in the yeast V-ATPase complex using cysteine-mediated cross-linking to subunit B. Biochemistry. 2002;41:11301-7 pubmed..Subunit D is thus the most likely homologue to the gamma subunit of F(1), which undergoes rotation during ATP hydrolysis and serves an essential function in rotary catalysis. ..
- Reenan R, Kolodner R. Characterization of insertion mutations in the Saccharomyces cerevisiae MSH1 and MSH2 genes: evidence for separate mitochondrial and nuclear functions. Genetics. 1992;132:975-85 pubmed..26%) PMS events were observed. These results indicate that MSH1 plays a role in repair or stability of mtDNA and MSH2 plays a role in repair of 4-bp insertion/deletion mispairs in the nucleus. ..
- Kumar S, Kao W, Howley P. Physical interaction between specific E2 and Hect E3 enzymes determines functional cooperativity. J Biol Chem. 1997;272:13548-54 pubmed
- Bao S, Chang M, Auclair D, Sun Y, Wang Y, Wong W, et al. HRad17, a human homologue of the Schizosaccharomyces pombe checkpoint gene rad17, is overexpressed in colon carcinoma. Cancer Res. 1999;59:2023-8 pubmed
- Martens J, Wu P, Winston F. Regulation of an intergenic transcript controls adjacent gene transcription in Saccharomyces cerevisiae. Genes Dev. 2005;19:2695-704 pubmed..Moreover, our results demonstrate a mechanism by which intergenic transcription allows activators to act indirectly as repressors. ..
- Keogh M, Kim J, Downey M, Fillingham J, Chowdhury D, Harrison J, et al. A phosphatase complex that dephosphorylates gammaH2AX regulates DNA damage checkpoint recovery. Nature. 2006;439:497-501 pubmed..The dephosphorylation of gammaH2AX by the HTP-C is necessary for efficient recovery from the DNA damage checkpoint. ..
- Kim T, Buratowski S. Two Saccharomyces cerevisiae JmjC domain proteins demethylate histone H3 Lys36 in transcribed regions to promote elongation. J Biol Chem. 2007;282:20827-35 pubmed..Taken together, these findings indicate that a general function of histone demethylases for H3 Lys(36) is to promote transcription elongation by antagonizing repressive Lys(36) methylation by Set2. ..
- Tzamarias D, Struhl K. Functional dissection of the yeast Cyc8-Tup1 transcriptional co-repressor complex. Nature. 1994;369:758-61 pubmed..We suggest that Tup1 performs the repression function of the Cyc8-Tup1 co-repressor complex, and that Cyc8 serves as a link with the pathway-specific DNA-binding proteins. ..
- Eisenmann D, Chapon C, Roberts S, Dollard C, Winston F. The Saccharomyces cerevisiae SPT8 gene encodes a very acidic protein that is functionally related to SPT3 and TATA-binding protein. Genetics. 1994;137:647-57 pubmed
- Gordon J, Shikov S, Kuehner J, Liriano M, Lee E, STAFFORD W, et al. Reconstitution of CF IA from overexpressed subunits reveals stoichiometry and provides insights into molecular topology. Biochemistry. 2011;50:10203-14 pubmed publisher..On the basis of our results and existing interaction data, we present a topological model for heterohexameric CF IA and its association with RNA and Hrp1. ..
- Yaglom J, Goldberg A, Finley D, Sherman M. The molecular chaperone Ydj1 is required for the p34CDC28-dependent phosphorylation of the cyclin Cln3 that signals its degradation. Mol Cell Biol. 1996;16:3679-84 pubmed..The reconstitution of this process with pure components provides evidence of a direct role for the chaperone in the phosphorylation of Cln3. ..
- Koepp D, Wong D, Corbett A, Silver P. Dynamic localization of the nuclear import receptor and its interactions with transport factors. J Cell Biol. 1996;133:1163-76 pubmed
- Dai Y, Wong B, Yen Y, Oettinger M, Kwon J, Johnson R. Determinants of HMGB proteins required to promote RAG1/2-recombination signal sequence complex assembly and catalysis during V(D)J recombination. Mol Cell Biol. 2005;25:4413-25 pubmed..The resulting complex must be sufficiently dynamic to enable the series of RAG1/2-mediated chemical reactions on the DNA. ..
- Prather D, Krogan N, Emili A, Greenblatt J, Winston F. Identification and characterization of Elf1, a conserved transcription elongation factor in Saccharomyces cerevisiae. Mol Cell Biol. 2005;25:10122-35 pubmed..Finally, purification of Elf1 suggests an association with casein kinase II, previously implicated in roles in transcription. Together, these results suggest an important role for Elf1 in the regulation of transcription elongation. ..
- Yoshida S, Bartolini S, Pellman D. Mechanisms for concentrating Rho1 during cytokinesis. Genes Dev. 2009;23:810-23 pubmed publisher..These findings comprehensively define the targeting mechanisms of Rho1 essential for cytokinesis in yeast, and are likely to be relevant to cytokinesis in other organisms. ..
- del Río Portilla F -, Gaskell A, Gilbert D, Ladias J, Wagner G. Solution structure of the hRPABC14.4 subunit of human RNA polymerases. Nat Struct Biol. 1999;6:1039-42 pubmed..4 structure accounts for mutagenesis results in Saccharomyces cerevisiae and provides a structural working model for elucidating the role of this subunit in the molecular architecture and function of the human nuclear RNA polymerases. ..
- Berger M, Philippakis A, Qureshi A, He F, Estep P, Bulyk M. Compact, universal DNA microarrays to comprehensively determine transcription-factor binding site specificities. Nat Biotechnol. 2006;24:1429-35 pubmed..The unbiased coverage of all k-mers permits high-throughput interrogation of binding site preferences, including nucleotide interdependencies, at unprecedented resolution. ..
- Hickman M, Spatt D, Winston F. The Hog1 mitogen-activated protein kinase mediates a hypoxic response in Saccharomyces cerevisiae. Genetics. 2011;188:325-38 pubmed publisher..Taken together, our results have uncovered a previously unknown hypoxic-response pathway that may be conserved throughout eukaryotes. ..
- Lechler T, Li R. In vitro reconstitution of cortical actin assembly sites in budding yeast. J Cell Biol. 1997;138:95-103 pubmed..It is composed of a novel protein, Pca1. Sequence analysis suggests that Pca1 has the potential to interact with SH3 domain-containing proteins and phospholipids. ..
- Seedorf M, Damelin M, Kahana J, Taura T, Silver P. Interactions between a nuclear transporter and a subset of nuclear pore complex proteins depend on Ran GTPase. Mol Cell Biol. 1999;19:1547-57 pubmed..These data indicate that transport receptors such as Pse1p interact in a Ran-dependent manner with certain nucleoporins. These nucleoporins may represent major docking sites for Pse1p as it moves in or out of the nucleus via the NPC. ..
- Lee L, Klee S, Evangelista M, Boone C, Pellman D. Control of mitotic spindle position by the Saccharomyces cerevisiae formin Bni1p. J Cell Biol. 1999;144:947-61 pubmed..Additionally, we present evidence that other bipolar bud site determinants together with cortical actin are also required for spindle orientation. ..
- Hidalgo P, Ansari A, Schmidt P, Hare B, Simkovich N, Farrell S, et al. Recruitment of the transcriptional machinery through GAL11P: structure and interactions of the GAL4 dimerization domain. Genes Dev. 2001;15:1007-20 pubmed..The binding of GAL4 to GAL11P, although an artificial interaction, represents a unique structural motif for an activating region capable of binding to a single target to effect gene expression. ..
- Jefferies K, Forgac M. Subunit H of the vacuolar (H+) ATPase inhibits ATP hydrolysis by the free V1 domain by interaction with the rotary subunit F. J Biol Chem. 2008;283:4512-9 pubmed..These results indicate that subunits H and F are proximal in free V1, supporting the hypothesis that subunit H inhibits free V1 by bridging the rotary and stator domains. ..