Genomes and Genes
Experts and Doctors on saccharomyces cerevisiae in Boston, Massachusetts, United States
Locale: Boston, Massachusetts, United States
Topic: saccharomyces cerevisiae
Publications173 found, 100 shown here
- Anderson R, Bitterman K, Wood J, Medvedik O, Sinclair D. Nicotinamide and PNC1 govern lifespan extension by calorie restriction in Saccharomyces cerevisiae. Nature. 2003;423:181-5 pubmed..We conclude that yeast lifespan extension by calorie restriction is the consequence of an active cellular response to a low-intensity stress and speculate that nicotinamide might regulate critical cellular processes in higher organisms. ..
- Katan Khaykovich Y, Struhl K. Heterochromatin formation involves changes in histone modifications over multiple cell generations. EMBO J. 2005;24:2138-49 pubmed..Thus, the transition between euchromatin and heterochromatin is gradual and requires multiple cell division cycles. ..
- Keogh M, Kim J, Downey M, Fillingham J, Chowdhury D, Harrison J, et al. A phosphatase complex that dephosphorylates gammaH2AX regulates DNA damage checkpoint recovery. Nature. 2006;439:497-501 pubmed..The dephosphorylation of gammaH2AX by the HTP-C is necessary for efficient recovery from the DNA damage checkpoint. ..
- Dendrinos K, Becker J, Stucchi A, Saubermann L, LaMorte W, Farraye F. Anti-Saccharomyces cerevisiae antibodies are associated with the development of postoperative fistulas following ileal pouch-anal anastomosis. J Gastrointest Surg. 2006;10:1060-4 pubmed..A larger study is needed to validate these observations. ..
- Proft M, Mas G, de Nadal E, Vendrell A, Noriega N, Struhl K, et al. The stress-activated Hog1 kinase is a selective transcriptional elongation factor for genes responding to osmotic stress. Mol Cell. 2006;23:241-50 pubmed..Thus, in addition to its various functions during transcriptional initiation, Hog1 behaves as a transcriptional elongation factor that is selective for genes induced upon osmotic stress. ..
- Shibata Y, Voss C, Rist J, Hu J, Rapoport T, Prinz W, et al. The reticulon and DP1/Yop1p proteins form immobile oligomers in the tubular endoplasmic reticulum. J Biol Chem. 2008;283:18892-904 pubmed publisher..We propose that oligomerization of the reticulons and DP1/Yop1p is important for both their localization to the tubular domains of the ER and for their ability to form tubules. ..
- Kasperkovitz P, Khan N, Tam J, Mansour M, Davids P, Vyas J. Toll-like receptor 9 modulates macrophage antifungal effector function during innate recognition of Candida albicans and Saccharomyces cerevisiae. Infect Immun. 2011;79:4858-67 pubmed publisher..Our data support a model in which orchestration of antifungal innate immunity involves a complex interplay of fungal ligand combinations, host cell machinery rearrangements, and TLR cooperation and antagonism. ..
- Galluzzi L, Baehrecke E, Ballabio A, Boya P, Bravo San Pedro J, Cecconi F, et al. Molecular definitions of autophagy and related processes. EMBO J. 2017;36:1811-1836 pubmed publisher..The ultimate objective of this collaborative exchange is to formulate recommendations that facilitate the dissemination of knowledge within and outside the field of autophagy research. ..
- Milne G, Ho T, Weaver D. Modulation of Saccharomyces cerevisiae DNA double-strand break repair by SRS2 and RAD51. Genetics. 1995;139:1189-99 pubmed..Thus, RAD52-dependent recombinational repair is controlled both negatively and positively. ..
- Lim A, Powers Lee S. Requirement for the carboxyl-terminal domain of Saccharomyces cerevisiae carbamoyl-phosphate synthetase. J Biol Chem. 1996;271:11400-9 pubmed..We also made the unexpected finding that, even when ammonia is used as the substrate and there is no catalytic role for CPA1, interaction with CPA1 led to an increase in the Vmax of CPA2 in crude extracts. ..
- Zhao J, Kessler M, Moore C. Cleavage factor II of Saccharomyces cerevisiae contains homologues to subunits of the mammalian Cleavage/ polyadenylation specificity factor and exhibits sequence-specific, ATP-dependent interaction with precursor RNA. J Biol Chem. 1997;272:10831-8 pubmed..These results provide additional evidence that certain features of the molecular mechanism of mRNA 3'-end formation are conserved between yeast and mammals, but also highlight unexpected differences. ..
- Rodriguez C, Cho E, Keogh M, Moore C, Greenleaf A, Buratowski S. Kin28, the TFIIH-associated carboxy-terminal domain kinase, facilitates the recruitment of mRNA processing machinery to RNA polymerase II. Mol Cell Biol. 2000;20:104-12 pubmed..Pta1 in yeast extracts binds specifically to the phosphorylated CTD, suggesting that this interaction may mediate coupling of polyadenylation and transcription. ..
- Hidalgo P, Ansari A, Schmidt P, Hare B, Simkovich N, Farrell S, et al. Recruitment of the transcriptional machinery through GAL11P: structure and interactions of the GAL4 dimerization domain. Genes Dev. 2001;15:1007-20 pubmed..The binding of GAL4 to GAL11P, although an artificial interaction, represents a unique structural motif for an activating region capable of binding to a single target to effect gene expression. ..
- Devroe E, Erdjument Bromage H, Tempst P, Silver P. Human Mob proteins regulate the NDR1 and NDR2 serine-threonine kinases. J Biol Chem. 2004;279:24444-51 pubmed..In summary, this work identifies a unique class of human kinase-activating subunits that may be functionally analagous to cyclins. ..
- de Carvalho C, COLAIACOVO M. SUMO-mediated regulation of synaptonemal complex formation during meiosis. Genes Dev. 2006;20:1986-92 pubmed
- Moore M, Schwartzfarb E, Silver P, Yu M. Differential recruitment of the splicing machinery during transcription predicts genome-wide patterns of mRNA splicing. Mol Cell. 2006;24:903-15 pubmed..Broadly, our results provide mechanistic insights into the coordinated regulation of transcription, mRNA processing, and nuclear export in executing complex gene expression programs. ..
- Liberman R, Cotter K, Baleja J, Forgac M. Structural analysis of the N-terminal domain of subunit a of the yeast vacuolar ATPase (V-ATPase) using accessibility of single cysteine substitutions to chemical modification. J Biol Chem. 2013;288:22798-808 pubmed publisher..The possible significance of these results for in vivo regulation of V-ATPase assembly is discussed. ..
- Luna R, Arthanari H, Hiraishi H, Akabayov B, Tang L, Cox C, et al. The interaction between eukaryotic initiation factor 1A and eIF5 retains eIF1 within scanning preinitiation complexes. Biochemistry. 2013;52:9510-8 pubmed publisher..These results suggest that the eIF1A-eIF5-CTD interaction during scanning PICs contributes to the maintenance of eIF1 within the open PIC...
- Eisenmann D, Arndt K, Ricupero S, Rooney J, Winston F. SPT3 interacts with TFIID to allow normal transcription in Saccharomyces cerevisiae. Genes Dev. 1992;6:1319-31 pubmed..Taken together, these results suggest that SPT3 is a TFIID-associated protein, required for TFIID to function at particular promoters in vivo. ..
- Kirchhausen T. Identification of a putative yeast homolog of the mammalian beta chains of the clathrin-associated protein complexes. Mol Cell Biol. 1990;10:6089-90 pubmed
- Wong D, Corbett A, Kent H, Stewart M, Silver P. Interaction between the small GTPase Ran/Gsp1p and Ntf2p is required for nuclear transport. Mol Cell Biol. 1997;17:3755-67 pubmed..Taken together, these data demonstrate that the interaction between Gsp1p and Ntf2p is critical for nuclear transport. ..
- Abeijon C, Chen L. The role of glucosidase I (Cwh41p) in the biosynthesis of cell wall beta-1,6-glucan is indirect. Mol Biol Cell. 1998;9:2729-38 pubmed..These results demonstrate that the role of glucosidase I (Cwh41p) in the biosynthesis of cell wall beta-1,6-glucan is indirect and that dolichol-P-glucose is not an intermediate in this pathway. ..
- Lee L, Klee S, Evangelista M, Boone C, Pellman D. Control of mitotic spindle position by the Saccharomyces cerevisiae formin Bni1p. J Cell Biol. 1999;144:947-61 pubmed..Additionally, we present evidence that other bipolar bud site determinants together with cortical actin are also required for spindle orientation. ..
- Geisberg J, Holstege F, Young R, Struhl K. Yeast NC2 associates with the RNA polymerase II preinitiation complex and selectively affects transcription in vivo. Mol Cell Biol. 2001;21:2736-42 pubmed..Thus, NC2 is associated with the Pol II preinitiation complex, and it can play a direct and positive role at certain promoters in vivo. ..
- Sheen V, Feng Y, Graham D, Takafuta T, Shapiro S, Walsh C. Filamin A and Filamin B are co-expressed within neurons during periods of neuronal migration and can physically interact. Hum Mol Genet. 2002;11:2845-54 pubmed..These results suggest that FLNA and FLNB may form both homodimers and heterodimers and that their interaction could potentially compensate for the loss of FLNA function during cortical development within PH individuals. ..
- Wu P, Ruhlmann C, Winston F, Schultz P. Molecular architecture of the S. cerevisiae SAGA complex. Mol Cell. 2004;15:199-208 pubmed..Our data provide insights into the molecular architecture of SAGA and imply a functional organization to the complex. ..
- Kim T, Buratowski S. Two Saccharomyces cerevisiae JmjC domain proteins demethylate histone H3 Lys36 in transcribed regions to promote elongation. J Biol Chem. 2007;282:20827-35 pubmed..Taken together, these findings indicate that a general function of histone demethylases for H3 Lys(36) is to promote transcription elongation by antagonizing repressive Lys(36) methylation by Set2. ..
- Mekhail K, Seebacher J, Gygi S, Moazed D. Role for perinuclear chromosome tethering in maintenance of genome stability. Nature. 2008;456:667-70 pubmed publisher..Our results therefore reveal an ancient mechanism in which interactions between INM proteins and chromosomal proteins ensure genome stability. ..
- Ezeokonkwo C, Zhelkovsky A, Lee R, Bohm A, Moore C. A flexible linker region in Fip1 is needed for efficient mRNA polyadenylation. RNA. 2011;17:652-64 pubmed publisher..Our results indicate that the Fip1 linker, through its flexibility and protein/protein interactions, allows Pap1 to reach the 3' end of the cleaved RNA and efficiently initiate poly(A) addition. ..
- Tishkoff D, Johnson A, Kolodner R. Molecular and genetic analysis of the gene encoding the Saccharomyces cerevisiae strand exchange protein Sep1. Mol Cell Biol. 1991;11:2593-608 pubmed..The interaction between sep1, rad50, and spo13 mutations suggested that SEP1 acts in meiosis in a pathway that is parallel to the RAD50 pathway. ..
- Swanson M, Carlson M, Winston F. SPT6, an essential gene that affects transcription in Saccharomyces cerevisiae, encodes a nuclear protein with an extremely acidic amino terminus. Mol Cell Biol. 1990;10:4935-41 pubmed..By use of an epitope-tagged SPT6 protein, we have determined by indirect immunofluorescence that the SPT6 protein is located in the nucleus. ..
- Chi N, Kolodner R. The effect of DNA mismatches on the ATPase activity of MSH1, a protein in yeast mitochondria that recognizes DNA mismatches. J Biol Chem. 1994;269:29993-7 pubmed..This interaction may provide a system for elucidating the role of ATP in mismatch recognition and repair. ..
- Maeda T, Tsai A, Saito H. Mutations in a protein tyrosine phosphatase gene (PTP2) and a protein serine/threonine phosphatase gene (PTC1) cause a synthetic growth defect in Saccharomyces cerevisiae. Mol Cell Biol. 1993;13:5408-17 pubmed..GST-PTC1 also had weak (approximately 0.5% of its serine phosphatase activity) protein tyrosine phosphatase activity. ..
- Corbett A, Silver P. The NTF2 gene encodes an essential, highly conserved protein that functions in nuclear transport in vivo. J Biol Chem. 1996;271:18477-84 pubmed..Taken together, these results demonstrate the exquisite functional conservation of this protein throughout evolution and indicate a critical in vivo role in nuclear transport. ..
- Benson J, Lawande R, Howley P. Conserved interaction of the papillomavirus E2 transcriptional activator proteins with human and yeast TFIIB proteins. J Virol. 1997;71:8041-7 pubmed..Finally, we demonstrate in vitro interaction between Saccharomyces cerevisiae TFIIB and BPV-1 E2, an observation that is consistent with the importance of the E2-TFIIB interaction for BPV-1 E2 transactivation in both systems. ..
- Kadosh D, Struhl K. Histone deacetylase activity of Rpd3 is important for transcriptional repression in vivo. Genes Dev. 1998;12:797-805 pubmed..These results suggest that the histone deacetylase activity of Rpd3 is important, but perhaps not absolutely required, for transcriptional repression in vivo. ..
- Ehmann D, Gehring A, Walsh C. Lysine biosynthesis in Saccharomyces cerevisiae: mechanism of alpha-aminoadipate reductase (Lys2) involves posttranslational phosphopantetheinylation by Lys5. Biochemistry. 1999;38:6171-7 pubmed..This is a novel mechanism for a fungal enzyme essential for amino acid metabolism. ..
- Larschan E, Winston F. The S. cerevisiae SAGA complex functions in vivo as a coactivator for transcriptional activation by Gal4. Genes Dev. 2001;15:1946-56 pubmed..These results, taken together with previous studies, demonstrate a dependent pathway for the recruitment of TBP to GAL gene promoters consisting of the recruitment of SAGA by Gal4 and the subsequent recruitment of TBP by SAGA. ..
- Keogh M, Cho E, Podolny V, Buratowski S. Kin28 is found within TFIIH and a Kin28-Ccl1-Tfb3 trimer complex with differential sensitivities to T-loop phosphorylation. Mol Cell Biol. 2002;22:1288-97 pubmed..Surprisingly, these phosphorylation site mutants appear to destabilize the association of the cyclin subunit within the context of TFIIH but not within the trimer complex. ..
- Martens J, Laprade L, Winston F. Intergenic transcription is required to repress the Saccharomyces cerevisiae SER3 gene. Nature. 2004;429:571-4 pubmed..This work identifies a previously unknown class of transcriptional regulatory genes. ..
- Mendelsohn R, Helmerhorst E, Cipollo J, Kukuruzinska M. A hypomorphic allele of the first N-glycosylation gene, ALG7, causes mitochondrial defects in yeast. Biochim Biophys Acta. 2005;1723:33-44 pubmed..Moreover, another N-glycosylation mutant with a LLO defect, alg6, was respiratory deficient. Collectively, our studies provide evidence that the dysregulation of N-glycosylation in haploid yeast cells leads to mitochondrial dysfunction. ..
- Cheslock P, Kemp B, Boumil R, Dawson D. The roles of MAD1, MAD2 and MAD3 in meiotic progression and the segregation of nonexchange chromosomes. Nat Genet. 2005;37:756-60 pubmed..These findings suggest plausible models for the basis of errant meiotic segregation in humans. ..
- Monahan B, Villen J, Marguerat S, Bahler J, Gygi S, Winston F. Fission yeast SWI/SNF and RSC complexes show compositional and functional differences from budding yeast. Nat Struct Mol Biol. 2008;15:873-80 pubmed publisher..Finally, phenotypic and microarray analyses identified widespread requirements for SWI/SNF and RSC on transcription including strong evidence that SWI/SNF directly represses iron-transport genes. ..
- Glass K, Huttenhower C, Quackenbush J, Yuan G. Passing messages between biological networks to refine predicted interactions. PLoS ONE. 2013;8:e64832 pubmed publisher..An implementation of the PANDA algorithm is available at www.sourceforge.net/projects/panda-net. ..
- Jin Y, Eser U, Struhl K, Churchman L. The Ground State and Evolution of Promoter Region Directionality. Cell. 2017;170:889-898.e10 pubmed publisher..Thus, promoter regions are intrinsically bidirectional and are shaped by evolution to bias transcription toward coding versus non-coding RNAs. ..
- Fishman Lobell J, Rudin N, Haber J. Two alternative pathways of double-strand break repair that are kinetically separable and independently modulated. Mol Cell Biol. 1992;12:1292-303 pubmed..4 kb. Moreover, the rate of deletion formation corresponds to the time at which complementary regions become single stranded. Gap repair processes are independent of distance but are reduced in rad52 mutants and in G1-arrested cells. ..
- Ng H, Feng Q, Wang H, Erdjument Bromage H, Tempst P, Zhang Y, et al. Lysine methylation within the globular domain of histone H3 by Dot1 is important for telomeric silencing and Sir protein association. Genes Dev. 2002;16:1518-27 pubmed..Our results indicate that histone modifications in the core globular domain have important biological functions. ..
- Berger M, Philippakis A, Qureshi A, He F, Estep P, Bulyk M. Compact, universal DNA microarrays to comprehensively determine transcription-factor binding site specificities. Nat Biotechnol. 2006;24:1429-35 pubmed..The unbiased coverage of all k-mers permits high-throughput interrogation of binding site preferences, including nucleotide interdependencies, at unprecedented resolution. ..
- Labunskyy V, Gerashchenko M, Delaney J, Kaya A, Kennedy B, Kaeberlein M, et al. Lifespan extension conferred by endoplasmic reticulum secretory pathway deficiency requires induction of the unfolded protein response. PLoS Genet. 2014;10:e1004019 pubmed publisher..These findings demonstrate that the UPR is an important determinant of lifespan that governs ER stress and identify a signaling network that couples stress resistance to longevity...
- Wei Y, Chen B, Samson L. Suppression of Escherichia coli alkB mutants by Saccharomyces cerevisiae genes. J Bacteriol. 1995;177:5009-15 pubmed..coli via an alkB-like mechanism remains to be determined, but protection appears to be specific for AlkB-deficient E. coli because none of the genes protect other alkylation-sensitive E. coli strains from killing by MMS. ..
- Nadeau K, Das A, Walsh C. Hsp90 chaperonins possess ATPase activity and bind heat shock transcription factors and peptidyl prolyl isomerases. J Biol Chem. 1993;268:1479-87 pubmed..We also detect binding of the other family of PPIases, the cyclophilins, to immobilized hsp90, consistent with a functional convergence of protein foldases. ..
- Kho C, Huggins G, Endege W, Hsieh C, Lee M, Haber E. Degradation of E2A proteins through a ubiquitin-conjugating enzyme, UbcE2A. J Biol Chem. 1997;272:3845-51 pubmed..Finally, antisense UbcE2A reduces E12 degradation. By participating in the degradation of the E2A proteins, UbcE2A may regulate cell growth and differentiation. ..
- Moqtaderi Z, Keaveney M, Struhl K. The histone H3-like TAF is broadly required for transcription in yeast. Mol Cell. 1998;2:675-82 pubmed
- He X, Khan A, Cheng H, Pappas D, Hampsey M, Moore C. Functional interactions between the transcription and mRNA 3' end processing machineries mediated by Ssu72 and Sub1. Genes Dev. 2003;17:1030-42 pubmed
- Wang Y, Elion E. Nuclear export and plasma membrane recruitment of the Ste5 scaffold are coordinated with oligomerization and association with signal transduction components. Mol Biol Cell. 2003;14:2543-58 pubmed
- Srinivasan S, Meyer R, Lugo R, Rahimi N. Identification of PDCL3 as a novel chaperone protein involved in the generation of functional VEGF receptor 2. J Biol Chem. 2013;288:23171-81 pubmed publisher..Taken together, our data provide strong evidence for the role of PDCL3 in angiogenesis and establishes the molecular mechanism by which it regulates VEGFR-2 expression and function. ..
- Sassanfar M, Samson L. Identification and preliminary characterization of an O6-methylguanine DNA repair methyltransferase in the yeast Saccharomyces cerevisiae. J Biol Chem. 1990;265:20-5 pubmed..Judging from its molecular weight and substrate specificity, the yeast DNA MTase is more closely related to mammalian MTases than to Escherichia coli MTases. ..
- Finley D, Bartel B, Varshavsky A. The tails of ubiquitin precursors are ribosomal proteins whose fusion to ubiquitin facilitates ribosome biogenesis. Nature. 1989;338:394-401 pubmed..These results suggest a novel 'chaperone' function for ubiquitin, in which its covalent association with other proteins promotes the formation of specific cellular structures. ..
- Tzamarias D, Struhl K. Functional dissection of the yeast Cyc8-Tup1 transcriptional co-repressor complex. Nature. 1994;369:758-61 pubmed..We suggest that Tup1 performs the repression function of the Cyc8-Tup1 co-repressor complex, and that Cyc8 serves as a link with the pathway-specific DNA-binding proteins. ..
- Lee D, Goldberg A. Selective inhibitors of the proteasome-dependent and vacuolar pathways of protein degradation in Saccharomyces cerevisiae. J Biol Chem. 1996;271:27280-4 pubmed..These two classes of inhibitors can thus be used to distinguish the cytosolic and vacuolar proteolytic pathways and to increase the cellular content of short-lived proteins. ..
- Quintana D, Hou Z, Thome K, Hendricks M, Saha P, Dutta A. Identification of HsORC4, a member of the human origin of replication recognition complex. J Biol Chem. 1997;272:28247-51 pubmed..HsORC4P is coimmunoprecipitated from cell extracts with another subunit of human ORC, HsORC2P, consistent with it being a part of the putative human origin recognition complex. ..
- Shen E, Henry M, Weiss V, Valentini S, Silver P, Lee M. Arginine methylation facilitates the nuclear export of hnRNP proteins. Genes Dev. 1998;12:679-91 pubmed..Together, these findings establish that one biological role for arginine methylation is in facilitating the export of certain hnRNPs out of the nucleus. ..
- Glickman M, Rubin D, Fried V, Finley D. The regulatory particle of the Saccharomyces cerevisiae proteasome. Mol Cell Biol. 1998;18:3149-62 pubmed..Overall, regulatory particles from yeasts and mammals are remarkably similar, suggesting that the specific mechanistic features of the proteasome have been closely conserved over the course of evolution. ..
- Matlack K, Misselwitz B, Plath K, Rapoport T. BiP acts as a molecular ratchet during posttranslational transport of prepro-alpha factor across the ER membrane. Cell. 1999;97:553-64 pubmed..Antibodies against the substrate can replace BiP, indicating that a Brownian ratchet is sufficient to achieve translocation. ..
- Xu T, Forgac M. Subunit D (Vma8p) of the yeast vacuolar H+-ATPase plays a role in coupling of proton transport and ATP hydrolysis. J Biol Chem. 2000;275:22075-81 pubmed..These results suggest that subunit D plays an important role in coupling of proton transport and ATP hydrolysis and that only low rates of turnover of the enzyme are required to support in vivo dissociation. ..
- Gao Q, Kumar A, Srinivasan S, Singh L, Mukai H, Ono Y, et al. PKN binds and phosphorylates human papillomavirus E6 oncoprotein. J Biol Chem. 2000;275:14824-30 pubmed..Finally, we show that PKN phosphorylates E6, demonstrating for the first time that HPV E6 is a phosphoprotein. Our finding suggests a novel link between HPV E6 mediated oncogenesis and regulation of a well known phosphorylation cascade. ..
- Gross S, Moore C. Rna15 interaction with the A-rich yeast polyadenylation signal is an essential step in mRNA 3'-end formation. Mol Cell Biol. 2001;21:8045-55 pubmed..These results illustrate that the yeast 3' end is defined and processed by a mechanism surprisingly different from that used by the mammalian system. ..
- Lei E, Silver P. Intron status and 3'-end formation control cotranscriptional export of mRNA. Genes Dev. 2002;16:2761-6 pubmed..Conversely, Yra1 recruitment to genes without introns is not dependent on splicing. Finally, 3'-end formation is required for Yra1 recruitment to genes regardless of intron status. ..
- Wang Y, Inoue T, Forgac M. Subunit a of the yeast V-ATPase participates in binding of bafilomycin. J Biol Chem. 2005;280:40481-8 pubmed..84 +/- 0.04 nm) that was slightly higher than wild-type (0.60 +/- 0.07 nm). These results suggest that subunit a of V-ATPase participates along with subunit c in binding bafilomycin. ..
- Lee Y, Elledge S. Control of ribonucleotide reductase localization through an anchoring mechanism involving Wtm1. Genes Dev. 2006;20:334-44 pubmed..In the presence of DNA damage this association is disrupted and Rnr2/Rnr4 become cytoplasmic, where they join with Rnr1 to form an intact complex. ..
- Duina A, Rufiange A, Bracey J, Hall J, Nourani A, Winston F. Evidence that the localization of the elongation factor Spt16 across transcribed genes is dependent upon histone H3 integrity in Saccharomyces cerevisiae. Genetics. 2007;177:101-12 pubmed
- Medvedik O, Lamming D, Kim K, Sinclair D. MSN2 and MSN4 link calorie restriction and TOR to sirtuin-mediated lifespan extension in Saccharomyces cerevisiae. PLoS Biol. 2007;5:e261 pubmed..These findings suggest that TOR and sirtuins may be part of the same longevity pathway in higher organisms, and that they may promote genomic stability during aging. ..
- Kadowaki T, Schneiter R, Hitomi M, Tartakoff A. Mutations in nucleolar proteins lead to nucleolar accumulation of polyA+ RNA in Saccharomyces cerevisiae. Mol Biol Cell. 1995;6:1103-10 pubmed..mRNA may normally encounter nucleolar components before export and proteins such as Mtr3p may be critical for export of both mRNA and ribosomal subunits. ..
- Roberts S, Winston F. Essential functional interactions of SAGA, a Saccharomyces cerevisiae complex of Spt, Ada, and Gcn5 proteins, with the Snf/Swi and Srb/mediator complexes. Genetics. 1997;147:451-65 pubmed..These findings suggest that SAGA has multiple activities and plays critical roles in transcription by RNA polymerase II. ..
- Werner E, Lee J, Hansen L, Yuan M, Shoelson S. Insulin resistance due to phosphorylation of insulin receptor substrate-1 at serine 302. J Biol Chem. 2004;279:35298-305 pubmed
- Hieronymus H, Yu M, Silver P. Genome-wide mRNA surveillance is coupled to mRNA export. Genes Dev. 2004;18:2652-62 pubmed..These results demonstrate coupling of mRNA surveillance to mRNA export and suggest specificity of the RNA surveillance machinery for different transcript populations. Broadly, these findings link DNA and RNA surveillance to mRNA export. ..
- Fresco L, Buratowski S. Conditional mutants of the yeast mRNA capping enzyme show that the cap enhances, but is not required for, mRNA splicing. RNA. 1996;2:584-96 pubmed..In addition, steady-state levels of several mRNAs were decreased, perhaps due to increased degradation of uncapped mRNAs. In contrast to splicing, mRNA polyadenylation and transport to the cytoplasm were unaffected. ..
- Keogh M, Mennella T, Sawa C, Berthelet S, Krogan N, Wolek A, et al. The Saccharomyces cerevisiae histone H2A variant Htz1 is acetylated by NuA4. Genes Dev. 2006;20:660-5 pubmed..Function-specific modifications may help explain how the same component of chromatin can function in diverse pathways. ..
- Libuda D, Winston F. Alterations in DNA replication and histone levels promote histone gene amplification in Saccharomyces cerevisiae. Genetics. 2010;184:985-97 pubmed publisher..Taken together, our results suggest that either reduced histone levels or slowed replication forks stimulate the HTA2-HTB2 amplification event, contributing to the restoration of normal chromatin structure. ..
- Deng W, Babu I, Su D, Yin S, Begley T, Dedon P. Trm9-Catalyzed tRNA Modifications Regulate Global Protein Expression by Codon-Biased Translation. PLoS Genet. 2015;11:e1005706 pubmed publisher..Together, these results suggest that Trm9-catalyzed tRNA modifications play a significant role in regulating protein expression within the cell. ..
- Qiu X, Lin Y, Thome K, Pian P, Schlegel B, Weremowicz S, et al. An eukaryotic RuvB-like protein (RUVBL1) essential for growth. J Biol Chem. 1998;273:27786-93 pubmed..Yeast with a null mutation in scRUVBL1 was nonviable. Thus RUVBL1 is an eukaryotic member of the RuvB/clamp loader family of structurally related proteins from bacteria and eukaryotes that is essential for viability of yeast. ..
- Kuras L, Struhl K. Binding of TBP to promoters in vivo is stimulated by activators and requires Pol II holoenzyme. Nature. 1999;399:609-13 pubmed
- King D, Hall B, Iwamoto M, Win K, Chang J, Ellenberger T. Domain structure and protein interactions of the silent information regulator Sir3 revealed by screening a nested deletion library of protein fragments. J Biol Chem. 2006;281:20107-19 pubmed..The resulting Sir3-Sir4 complexes form progressively higher order assemblies with increasing protein concentration, with implications for the mechanism of gene silencing. ..
- Reibarkh M, Yamamoto Y, Singh C, del Rio F, Fahmy A, Lee B, et al. Eukaryotic initiation factor (eIF) 1 carries two distinct eIF5-binding faces important for multifactor assembly and AUG selection. J Biol Chem. 2008;283:1094-103 pubmed..Thus, eIF5 is an excellent candidate for the direct partner of eIF1-KH that mediates the critical link. The direct interaction at eIF1-KH also places eIF5 near the decoding site of the 40 S subunit. ..
- Kessler M, Zhao J, Moore C. Purification of the Saccharomyces cerevisiae cleavage/polyadenylation factor I. Separation into two components that are required for both cleavage and polyadenylation of mRNA 3' ends. J Biol Chem. 1996;271:27167-75 pubmed..RNA15 is the only subunit of CF I that can be cross-linked to pre-mRNA. ..
- Cherkasova V, Lyons D, Elion E. Fus3p and Kss1p control G1 arrest in Saccharomyces cerevisiae through a balance of distinct arrest and proliferative functions that operate in parallel with Far1p. Genetics. 1999;151:989-1004 pubmed..Thus, Fus3p and Kss1p control G1 arrest through a balance of arrest functions that inhibit the Cdc28p machinery and proliferative functions that bypass this inhibition. ..
- Farley F, Satterberg B, Goldsmith E, Elion E. Relative dependence of different outputs of the Saccharomyces cerevisiae pheromone response pathway on the MAP kinase Fus3p. Genetics. 1999;151:1425-44 pubmed..Finally, suppression analysis argues that Kss1p contributes to the overall pheromone response in a wild-type strain, but that Fus3p is the critical kinase for all of the outputs tested. ..
- Shanks R, Kamieniecki R, Dawson D. The Kar3-interacting protein Cik1p plays a critical role in passage through meiosis I in Saccharomyces cerevisiae. Genetics. 2001;159:939-51 pubmed..Cellular localization experiments reveal that Kar3p, Cik1p, and Vik1p are present throughout meiosis and are consistent with Cik1p and Vik1p having different meiotic roles. ..
- Ng H, Dole S, Struhl K. The Rtf1 component of the Paf1 transcriptional elongation complex is required for ubiquitination of histone H2B. J Biol Chem. 2003;278:33625-8 pubmed..We suggest that Rtf1 affects genome-wide ubiquitination of H2B by a mechanism that is distinct from its function as a transcriptional elongation factor. ..