Experts and Doctors on molecular sequence data in Cambridge, Massachusetts, United States

Summary

Locale: Cambridge, Massachusetts, United States
Topic: molecular sequence data

Top Publications

  1. Guo H, Jardetzky T, Garrett T, Lane W, Strominger J, Wiley D. Different length peptides bind to HLA-Aw68 similarly at their ends but bulge out in the middle. Nature. 1992;360:364-6 pubmed
  2. Zamore P, Patton J, Green M. Cloning and domain structure of the mammalian splicing factor U2AF. Nature. 1992;355:609-14 pubmed
    ..It contains two functional domains: a sequence-specific RNA-binding region composed of three ribonucleoprotein-consensus sequence domains, and an arginine/serine-rich motif necessary for splicing but not for binding to pre-mRNA. ..
  3. Koyama S, Yu H, Dalgarno D, Shin T, Zydowsky L, Schreiber S. Structure of the PI3K SH3 domain and analysis of the SH3 family. Cell. 1993;72:945-52 pubmed
    ..The results suggest that while the overall structures of the binding sites in the PI3K and Src SH3 domains are similar, their ligand binding properties may differ. ..
  4. Mäkelä T, Tassan J, Nigg E, Frutiger S, Hughes G, Weinberg R. A cyclin associated with the CDK-activating kinase MO15. Nature. 1994;371:254-7 pubmed
    ..These findings demonstrate that a cyclin/kinase complex can function as a regulator of other cyclin/kinase complexes, and suggest that cyclin/kinase cascades may exist. ..
  5. Cottarel G. Mcs4, a two-component system response regulator homologue, regulates the Schizosaccharomyces pombe cell cycle control. Genetics. 1997;147:1043-51 pubmed
    ..These findings link the two-component signal transduction system to stress response and cell cycle control in S. pombe. ..
  6. Bertin J, Guo Y, Wang L, Srinivasula S, Jacobson M, Poyet J, et al. CARD9 is a novel caspase recruitment domain-containing protein that interacts with BCL10/CLAP and activates NF-kappa B. J Biol Chem. 2000;275:41082-6 pubmed
    ..CARD9 also self-associates and contains extensive coiled-coil motifs that may function as oligomerization domains. We propose here that CARD9 is an upstream activator of BCL10 and NF-kappaB signaling. ..
  7. Burgon P, Lee W, Nixon A, Peralta E, Casey P. Phosphorylation and nuclear translocation of a regulator of G protein signaling (RGS10). J Biol Chem. 2001;276:32828-34 pubmed
    ..Rather, the phosphorylation event correlates with translocation of RGS10 from the plasma membrane and cytosol into the nucleus. ..
  8. Norman D, Chung S, Verdine G. Structural and biochemical exploration of a critical amino acid in human 8-oxoguanine glycosylase. Biochemistry. 2003;42:1564-72 pubmed
    ..Crystal structures of these mutants complexed with an unreactive abasic site in DNA reveal these residues to adopt a sterically disfavored helix-capping conformation. ..
  9. Neafsey D, Palumbi S. Genome size evolution in pufferfish: a comparative analysis of diodontid and tetraodontid pufferfish genomes. Genome Res. 2003;13:821-30 pubmed
    ..We comment on the role that large insertions may play in the evolution of genome size in these two groups...

More Information

Publications197 found, 100 shown here

  1. Towler P, Staker B, Prasad S, Menon S, Tang J, Parsons T, et al. ACE2 X-ray structures reveal a large hinge-bending motion important for inhibitor binding and catalysis. J Biol Chem. 2004;279:17996-8007 pubmed
  2. Fujino T, Wu Z, Lin W, Phillips M, Nedivi E. cpg15 and cpg15-2 constitute a family of activity-regulated ligands expressed differentially in the nervous system to promote neurite growth and neuronal survival. J Comp Neurol. 2008;507:1831-45 pubmed publisher
    ..Our findings suggest that CPG15 and CPG15-2 perform similar cellular functions but may play distinct roles in vivo through their cell-type- and tissue-specific transcriptional regulation. ..
  3. Fire E, Gulla S, Grant R, Keating A. Mcl-1-Bim complexes accommodate surprising point mutations via minor structural changes. Protein Sci. 2010;19:507-19 pubmed publisher
  4. Lohman G, Stubbe J. Inactivation of Lactobacillus leichmannii ribonucleotide reductase by 2',2'-difluoro-2'-deoxycytidine 5'-triphosphate: covalent modification. Biochemistry. 2010;49:1404-17 pubmed publisher
    ..1021/bi9021318 ) allow proposal of a mechanism of inactivation of RNR by F(2)CTP involving multiple reaction pathways. The proposed mechanisms share many common features with F(2)CDP inactivation of the class I RNRs. ..
  5. Carroll S, Ortlund E, Thornton J. Mechanisms for the evolution of a derived function in the ancestral glucocorticoid receptor. PLoS Genet. 2011;7:e1002117 pubmed publisher
    ..Taken together, our results highlight the potentially creative role of substitutions that partially degrade protein structure and function and reinforce the importance of permissive mutations in protein evolution...
  6. Bradley R, Merkin J, Lambert N, Burge C. Alternative splicing of RNA triplets is often regulated and accelerates proteome evolution. PLoS Biol. 2012;10:e1001229 pubmed publisher
  7. Slater S, Wold S, Lu M, Boye E, Skarstad K, Kleckner N. E. coli SeqA protein binds oriC in two different methyl-modulated reactions appropriate to its roles in DNA replication initiation and origin sequestration. Cell. 1995;82:927-36 pubmed
    ..Also, binding of hemimethylated oriC by membrane fractions is due to SeqA. Direct interaction of SeqA protein with the replication origin is likely to be involved in both replication initiation and sequestration...
  8. Sevier C, Cuozzo J, Vala A, Aslund F, Kaiser C. A flavoprotein oxidase defines a new endoplasmic reticulum pathway for biosynthetic disulphide bond formation. Nat Cell Biol. 2001;3:874-82 pubmed
  9. LeVier K, Walker G. Genetic analysis of the Sinorhizobium meliloti BacA protein: differential effects of mutations on phenotypes. J Bacteriol. 2001;183:6444-53 pubmed
    ..The remaining 12 site-directed mutants demonstrate mixed phenotypic characteristics and fall into a number of distinctly different groups. These observations may be consistent with a role for BacA in multiple, nonoverlapping functions. ..
  10. Scarr R, Sharp P. PDCD2 is a negative regulator of HCF-1 (C1). Oncogene. 2002;21:5245-54 pubmed
    ..Overexpression of domains of either PDCD2 or HCF-1, which should interfere with interactions between these two proteins, enhances the complementation. ..
  11. Axtell M, Bartel D. Antiquity of microRNAs and their targets in land plants. Plant Cell. 2005;17:1658-73 pubmed
    ..Therefore, several individual miRNA regulatory circuits have ancient origins and have remained intact throughout the evolution and diversification of plants. ..
  12. Giddings L, Liscombe D, Hamilton J, Childs K, DellaPenna D, Buell C, et al. A stereoselective hydroxylation step of alkaloid biosynthesis by a unique cytochrome P450 in Catharanthus roseus. J Biol Chem. 2011;286:16751-7 pubmed publisher
    ..The discovery of CYP71BJ1, which forms part of the pathway leading to 19-O-acetylhörhammericine, will help illuminate how this branch point is controlled in C. roseus. ..
  13. Chicz R, Urban R, Lane W, Gorga J, Stern L, Vignali D, et al. Predominant naturally processed peptides bound to HLA-DR1 are derived from MHC-related molecules and are heterogeneous in size. Nature. 1992;358:764-8 pubmed
  14. Choder M. A general topoisomerase I-dependent transcriptional repression in the stationary phase in yeast. Genes Dev. 1991;5:2315-26 pubmed
    ..A minor population of mRNAs is not subjected to the TOP1-dependent regulation, as their levels do not change in stationary phase. The possible role of topoisomerase I in the general transcriptional repression is discussed. ..
  15. Martinez Cruzado J. Evolution of the autosomal chorion cluster in Drosophila. IV. The Hawaiian Drosophila: rapid protein evolution and constancy in the rate of DNA divergence. J Mol Evol. 1990;31:402-23 pubmed
    ..8% million years, whereas synonymous sites are substituted at a rate of 1.2% million years. ..
  16. Gouaux J, Lipscomb W. Crystal structures of phosphonoacetamide ligated T and phosphonoacetamide and malonate ligated R states of aspartate carbamoyltransferase at 2.8-A resolution and neutral pH. Biochemistry. 1990;29:389-402 pubmed
    ..Crystallographic residuals refined to 0.16-0.18 at 2.8-A resolution for the three structures. ..
  17. McAlear M, Howell E, Espenshade K, Holm C. Proliferating cell nuclear antigen (pol30) mutations suppress cdc44 mutations and identify potential regions of interaction between the two encoded proteins. Mol Cell Biol. 1994;14:4390-7 pubmed
    ..This face identifies a region likely to be important for functional interaction between the CDC44 and POL30 gene products. ..
  18. Grether M, Abrams J, Agapite J, White K, Steller H. The head involution defective gene of Drosophila melanogaster functions in programmed cell death. Genes Dev. 1995;9:1694-708 pubmed
    ..This phenotype can be suppressed completely by expression of the anti-apoptotic p35 protein from baculovirus, indicating that p35 may act genetically downstream from hid. ..
  19. Deng H, Lee J, Goldstein L, Branton D. Drosophila development requires spectrin network formation. J Cell Biol. 1995;128:71-9 pubmed
    ..Our results show that spectrin's capacity to form a network is a crucial aspect of its function in nonerythroid cells. ..
  20. Liao S, Zhang J, Jeffery D, Koleske A, Thompson C, Chao D, et al. A kinase-cyclin pair in the RNA polymerase II holoenzyme. Nature. 1995;374:193-6 pubmed
    ..These results indicate that the SRB10/11 kinase is involved in CTD phosphorylation and suggest that this modification has a role in the response to transcriptional regulators in vivo. ..
  21. Aiba A, Chen C, Herrup K, Rosenmund C, Stevens C, Tonegawa S. Reduced hippocampal long-term potentiation and context-specific deficit in associative learning in mGluR1 mutant mice. Cell. 1994;79:365-75 pubmed
    ..We propose that mGluR1 is not "in line" in LTP production, but rather modulates the plasticity process, and hence affects context-specific associative learning. ..
  22. Tang Z, Scherer P, Okamoto T, Song K, Chu C, Kohtz D, et al. Molecular cloning of caveolin-3, a novel member of the caveolin gene family expressed predominantly in muscle. J Biol Chem. 1996;271:2255-61 pubmed
    ..Our results also suggest that other as yet unknown caveolin family members are likely to exist and may be expressed in a regulated or tissue-specific fashion. ..
  23. Korinek W, Bi E, Epp J, Wang L, Ho J, Chant J. Cyk3, a novel SH3-domain protein, affects cytokinesis in yeast. Curr Biol. 2000;10:947-50 pubmed
    ..Thus, Cyk3 appears to be important for cytokinesis and acts potentially downstream of Iqg1. ..
  24. Sutton M, Farrow M, Burton B, Walker G. Genetic interactions between the Escherichia coli umuDC gene products and the beta processivity clamp of the replicative DNA polymerase. J Bacteriol. 2001;183:2897-909 pubmed
    ..Four possible models for how interactions of UmuD(2)C with the epsilon and the beta subunits of DNA polymerase III might help to regulate DNA replication in response to DNA damage are discussed. ..
  25. Giribet G, Edgecombe G, Wheeler W. Arthropod phylogeny based on eight molecular loci and morphology. Nature. 2001;413:157-61 pubmed
    ..The optimal 'total evidence' cladogram supports the crustacean-hexapod clade, recognizes pycnogonids as sister to other euarthropods, and indicates monophyly of Myriapoda and Mandibulata. ..
  26. Hwang H, Olson S, Esko J, Horvitz H. Caenorhabditis elegans early embryogenesis and vulval morphogenesis require chondroitin biosynthesis. Nature. 2003;423:439-43 pubmed
    ..Our findings show that chondroitin is crucial for both cytokinesis and morphogenesis during C. elegans development. ..
  27. Taylor N, Ge Z, Shen Z, Dewhirst F, Fox J. Cytolethal distending toxin: a potential virulence factor for Helicobacter cinaedi. J Infect Dis. 2003;188:1892-7 pubmed
    ..cinaedi isolates. H. cinaedi shares the production of Cdt with other enteric pathogens, including enterohepatic Helicobacter species. This is the first report of a putative virulence factor in H. cinaedi...
  28. Siddiqui S, Sauer R, Baker T. Role of the processing pore of the ClpX AAA+ ATPase in the recognition and engagement of specific protein substrates. Genes Dev. 2004;18:369-74 pubmed
    ..These results demonstrate that the ClpX pore functions in the recognition and catalytic engagement of specific substrates, and that ClpX recognizes different substrate classes in at least two distinct fashions. ..
  29. Burdick J, Chung C, Jia X, Randolph M, Langer R. Controlled degradation and mechanical behavior of photopolymerized hyaluronic acid networks. Biomacromolecules. 2005;6:386-91 pubmed
    ..Finally, auricular swine chondrocytes produced neocartilage when photoencapsulated in the hyaluronic acid networks. This work presents a next step toward the development of advanced in vivo curable biomaterials...
  30. Mutsuddi M, Chaffee B, Cassidy J, Silver S, Tootle T, Rebay I. Using Drosophila to decipher how mutations associated with human branchio-oto-renal syndrome and optical defects compromise the protein tyrosine phosphatase and transcriptional functions of eyes absent. Genetics. 2005;170:687-95 pubmed
  31. Fernandez Suarez M, Chen T, Ting A. Protein-protein interaction detection in vitro and in cells by proximity biotinylation. J Am Chem Soc. 2008;130:9251-3 pubmed publisher
    ..Protein-protein interaction detection by proximity biotinylation has the advantages of low background, high sensitivity, small AP tag size, and good spatial resolution in cells. ..
  32. Shakey Q, Bates B, Wu J. An approach to quantifying N-linked glycoproteins by enzyme-catalyzed 18O3-labeling of solid-phase enriched glycopeptides. Anal Chem. 2010;82:7722-8 pubmed publisher
    ..The strategy can be easily adapted to the analysis of glycoproteins in tissues, cell lines, and other sample origins. ..
  33. Woo L, Egner P, Belanger C, Wattanawaraporn R, Trudel L, Croy R, et al. Aflatoxin B1-DNA adduct formation and mutagenicity in livers of neonatal male and female B6C3F1 mice. Toxicol Sci. 2011;122:38-44 pubmed publisher
    ..The gender difference in the subsequent development of HCC highlights the importance of elucidating additional factors that modulate HCC development. ..
  34. DeLaBarre B, Gross S, Fang C, Gao Y, Jha A, Jiang F, et al. Full-length human glutaminase in complex with an allosteric inhibitor. Biochemistry. 2011;50:10764-70 pubmed publisher
    ..The importance of these loops with regard to overall enzymatic activity of the tetramer was revealed by a series of GAC point mutants designed to create a BPTES resistant GAC. ..
  35. Gavin J, Chen J, Liao H, Rollins N, Yang X, Xu Q, et al. Mechanistic studies on activation of ubiquitin and di-ubiquitin-like protein, FAT10, by ubiquitin-like modifier activating enzyme 6, Uba6. J Biol Chem. 2012;287:15512-22 pubmed publisher
    ..Together with expression level analysis in IM-9 cells, this study sheds light on the potential role of cytokine-induced FAT10 expression in regulating Uba6 pathways. ..
  36. Neafsey D, Galinsky K, Jiang R, Young L, Sykes S, Saif S, et al. The malaria parasite Plasmodium vivax exhibits greater genetic diversity than Plasmodium falciparum. Nat Genet. 2012;44:1046-50 pubmed publisher
    ..vivax. These findings warrant a deeper survey of variation in P. vivax to equip disease interventions targeting the distinctive biology of this neglected but major pathogen. ..
  37. Kovalenko O, Olland A, Piche Nicholas N, Godbole A, King D, Svenson K, et al. Atypical antigen recognition mode of a shark immunoglobulin new antigen receptor (IgNAR) variable domain characterized by humanization and structural analysis. J Biol Chem. 2013;288:17408-19 pubmed publisher
    ..This information broadens the general understanding of antigen recognition and provides a framework for further design and humanization of shark IgNARs. ..
  38. Meziane O, Piquet S, Bossé G, Gagné D, Paquet E, Robert C, et al. The human decapping scavenger enzyme DcpS modulates microRNA turnover. Sci Rep. 2015;5:16688 pubmed publisher
    ..We also demonstrate that this new function for DcpS requires the contribution of the 5'-3' exonuclease Xrn2. Our findings support a conserved role of DcpS as a modulator of miRNA turnover in animals. ..
  39. Goelz S, Hession C, Goff D, Griffiths B, Tizard R, Newman B, et al. ELFT: a gene that directs the expression of an ELAM-1 ligand. Cell. 1990;63:1349-56 pubmed
    ..Furthermore, ELFT is expressed specifically in cell types that bind to ELAM-1, suggesting that this enzyme is an important regulator of inflammatory events in vivo. ..
  40. Cutting S, Zheng L, Losick R. Gene encoding two alkali-soluble components of the spore coat from Bacillus subtilis. J Bacteriol. 1991;173:2915-9 pubmed
    ..The map position of cotF is 349 degrees. Transcription of cotF commenced coincidently (during h 6 of sporulation) with genes known to be under the control of sporulation transcription factor sigma kappa. ..
  41. Zheng L, Donovan W, Fitz James P, Losick R. Gene encoding a morphogenic protein required in the assembly of the outer coat of the Bacillus subtilis endospore. Genes Dev. 1988;2:1047-54 pubmed
  42. Chen L, Frankel A, Harder J, Fawell S, Barsoum J, Pepinsky B. Increased cellular uptake of the human immunodeficiency virus-1 Tat protein after modification with biotin. Anal Biochem. 1995;227:168-75 pubmed
    ..The increased uptake of Tat and Tat conjugates by addition of hydrophobic groups may significantly enhance the usefulness of Tat as a delivery vehicle, and the approach may be applicable to other systems. ..
  43. Robison K, Gilbert W, Church G. Large scale bacterial gene discovery by similarity search. Nat Genet. 1994;7:205-14 pubmed publisher
    ..Seven proteins are members of families which have not been described in prokaryotic sequences. We also describe 49 re-interpretations of existing sequence data of particular biological significance...
  44. Palombella V, Rando O, Goldberg A, Maniatis T. The ubiquitin-proteasome pathway is required for processing the NF-kappa B1 precursor protein and the activation of NF-kappa B. Cell. 1994;78:773-85 pubmed
    ..Thus, the ubiquitin-proteasome pathway functions not only in the complete degradation of polypeptides, but also in the regulated processing of precursors into active proteins. ..
  45. Shyjan A, Heldin P, Butcher E, Yoshino T, Briskin M. Functional cloning of the cDNA for a human hyaluronan synthase. J Biol Chem. 1996;271:23395-9 pubmed
  46. Schievella A, Chen J, Graham J, Lin L. MADD, a novel death domain protein that interacts with the type 1 tumor necrosis factor receptor and activates mitogen-activated protein kinase. J Biol Chem. 1997;272:12069-75 pubmed
    ..These data indicate that MADD links TNFR1 with MAP kinase activation and arachidonic acid release and provide further insight into the mechanisms by which TNF exerts its pleiotropic effects. ..
  47. Chou J, Li H, Salvesen G, Yuan J, Wagner G. Solution structure of BID, an intracellular amplifier of apoptotic signaling. Cell. 1999;96:615-24 pubmed
    ..Additionally, we show that the overall structure of BID is preserved after cleavage by Caspase 8. We propose that BID has both BH3 domain-dependent and -independent modes of action in inducing mitochondrial damage. ..
  48. Xu X, Nakano T, Wick S, Dubay M, Brizuela L. Mechanism of Cdk2/Cyclin E inhibition by p27 and p27 phosphorylation. Biochemistry. 1999;38:8713-22 pubmed
  49. Willis L, Walker G. A novel Sinorhizobium meliloti operon encodes an alpha-glucosidase and a periplasmic-binding-protein-dependent transport system for alpha-glucosides. J Bacteriol. 1999;181:4176-84 pubmed
  50. Lugovskoy A, Zhou P, Chou J, McCarty J, Li P, Wagner G. Solution structure of the CIDE-N domain of CIDE-B and a model for CIDE-N/CIDE-N interactions in the DNA fragmentation pathway of apoptosis. Cell. 1999;99:747-55 pubmed
    ..Based on these results and mutagenesis data, we propose a model for the CIDE-N/ CIDE-N complex and discuss the role of this novel bipolar interaction in mediating downstream events of apoptosis. ..
  51. Youn H, Grozinger C, Liu J. Calcium regulates transcriptional repression of myocyte enhancer factor 2 by histone deacetylase 4. J Biol Chem. 2000;275:22563-7 pubmed
    ..Together, HDAC4, MITR, and Cabin1 constitute a family of calcium-sensitive transcriptional repressors of MEF2. ..
  52. Rubinson D, Dillon C, Kwiatkowski A, Sievers C, Yang L, Kopinja J, et al. A lentivirus-based system to functionally silence genes in primary mammalian cells, stem cells and transgenic mice by RNA interference. Nat Genet. 2003;33:401-6 pubmed
    ..They may also provide new approaches for gene therapy. ..
  53. de la Cruz I, Levin J, Cummins C, Anderson P, Horvitz H. sup-9, sup-10, and unc-93 may encode components of a two-pore K+ channel that coordinates muscle contraction in Caenorhabditis elegans. J Neurosci. 2003;23:9133-45 pubmed
    ..elegans, Drosophila, and humans. Our results indicate that SUP-9 and perhaps other two-pore K+ channels function as multiprotein complexes, and that UNC-93 and SUP-10 likely define new classes of ion channel regulatory proteins. ..
  54. Lin C, Jao C, Ting A. Genetically encoded fluorescent reporters of histone methylation in living cells. J Am Chem Soc. 2004;126:5982-3 pubmed
    ..These reporters should be useful for studying gene silencing and X-chromosome inactivation with high spatial and temporal resolution in intact cells and may also aid in the search for conjectured histone demethylase activity. ..
  55. Neafsey D, Blumenstiel J, Hartl D. Different regulatory mechanisms underlie similar transposable element profiles in pufferfish and fruitflies. Mol Biol Evol. 2004;21:2310-8 pubmed
    ..Inference of regulatory processes governing TE profiles should take into account factors such as effective population size, incidence of inbreeding/outcrossing, and other species-specific traits. ..
  56. Jacobs M, Black J, Futer O, Swenson L, Hare B, Fleming M, et al. Pim-1 ligand-bound structures reveal the mechanism of serine/threonine kinase inhibition by LY294002. J Biol Chem. 2005;280:13728-34 pubmed
    ..Although the phosphatidylinositol 3-kinase inhibitor LY294002 also inhibits Pim-1, the structure of the LY294002.Pim-1 complex reveals a new binding mode that may be general for Ser/Thr kinases. ..
  57. Palmer Toy D, Krastins B, Sarracino D, Nadol J, Merchant S. Efficient method for the proteomic analysis of fixed and embedded tissues. J Proteome Res. 2005;4:2404-11 pubmed
    ..Moreover, we demonstrate that a high level of sequence coverage can be observed for proteins of interest. ..
  58. Wen D, Wildes C, Silvian L, Walus L, Mi S, Lee D, et al. Disulfide structure of the leucine-rich repeat C-terminal cap and C-terminal stalk region of Nogo-66 receptor. Biochemistry. 2005;44:16491-501 pubmed
    ..A structural model of the LRRCT with extended residues 311-344 from the CT stalk region is proposed, and its function in coreceptor binding is discussed. ..
  59. McGinness K, Baker T, Sauer R. Engineering controllable protein degradation. Mol Cell. 2006;22:701-7 pubmed
  60. Myers S, Freeman C, Auton A, Donnelly P, McVean G. A common sequence motif associated with recombination hot spots and genome instability in humans. Nat Genet. 2008;40:1124-9 pubmed publisher
  61. Konstantinidis K, Braff J, Karl D, DeLong E. Comparative metagenomic analysis of a microbial community residing at a depth of 4,000 meters at station ALOHA in the North Pacific subtropical gyre. Appl Environ Microbiol. 2009;75:5345-55 pubmed publisher
  62. Tinoco A, Kim Y, Tagore D, Wiwczar J, Lane W, Danial N, et al. A peptidomics strategy to elucidate the proteolytic pathways that inactivate peptide hormones. Biochemistry. 2011;50:2213-22 pubmed publisher
    ..Moreover, integrating this peptidomics approach with bioassays (i.e., GSIS) provides a general strategy to reveal proteolytic pathways that may regulate the activity of peptide hormones. ..
  63. Zhong X, Cooley C, Seth N, Juo Z, Presman E, Resendes N, et al. Engineering novel Lec1 glycosylation mutants in CHO-DUKX cells: molecular insights and effector modulation of N-acetylglucosaminyltransferase I. Biotechnol Bioeng. 2012;109:1723-34 pubmed publisher
    ..The data support the hypothesis that modulating GnTI activity can influence antibody effector functions for proteins with an IgG1 immunoglobulin Fc domain. ..
  64. Martinez D, Oliver B, Gräser Y, Goldberg J, Li W, Martinez Rossi N, et al. Comparative genome analysis of Trichophyton rubrum and related dermatophytes reveals candidate genes involved in infection. MBio. 2012;3:e00259-12 pubmed publisher
    ..These genome analyses identify gene families that are important to our understanding of how dermatophytes cause chronic infections, how they interact with epithelial cells, and how they respond to the host immune response. ..
  65. Moon T, Lou C, Tamsir A, Stanton B, Voigt C. Genetic programs constructed from layered logic gates in single cells. Nature. 2012;491:249-53 pubmed publisher
    ..This work demonstrates the successful layering of orthogonal logic gates, a design strategy that could enable the construction of large, integrated circuits in single cells. ..
  66. McKee A, Kleckner N. Mutations in Saccharomyces cerevisiae that block meiotic prophase chromosome metabolism and confer cell cycle arrest at pachytene identify two new meiosis-specific genes SAE1 and SAE3. Genetics. 1997;146:817-34 pubmed
    ..SAE1 encodes a 208-residue protein homologous to vertebrate mRNA cap-binding protein 20. SAE3 corresponds to a meiosis-specific RNA encoding an unusually short open reading frame of 50 codons. ..
  67. Peterson R, Schreiber S. Kinase phosphorylation: Keeping it all in the family. Curr Biol. 1999;9:R521-4 pubmed
    ..Recent findings suggest that the elusive PDK2 may actually be a familiar kinase with an atypical associate. ..
  68. Xu H, Dembski M, Yang Q, Yang D, Moriarty A, Tayber O, et al. Dual specificity mitogen-activated protein (MAP) kinase phosphatase-4 plays a potential role in insulin resistance. J Biol Chem. 2003;278:30187-92 pubmed
    ..Our data suggest that MKP-4 negatively regulates insulin signaling and, consequently, may contribute to the pathogenesis of insulin resistance. ..
  69. Hoyle J, Tang Y, Wiellette E, Wardle F, Sive H. nlz gene family is required for hindbrain patterning in the zebrafish. Dev Dyn. 2004;229:835-46 pubmed
    ..These data indicate that one function of the nlz gene family is to specify or maintain r4 identity, and to limit r3 and r5 during hindbrain formation. ..
  70. Nalefski E, Sultzman L, Martin D, Kriz R, Towler P, Knopf J, et al. Delineation of two functionally distinct domains of cytosolic phospholipase A2, a regulatory Ca(2+)-dependent lipid-binding domain and a Ca(2+)-independent catalytic domain. J Biol Chem. 1994;269:18239-49 pubmed
    ..These results delineate two functionally distinct domains of cPLA2, the Ca(2+)-independent catalytic domain, and the regulatory CaLB domain that presents the catalytic domain to the membrane in response to elevated Ca2+. ..
  71. Schorle H, Meier P, Buchert M, Jaenisch R, Mitchell P. Transcription factor AP-2 essential for cranial closure and craniofacial development. Nature. 1996;381:235-8 pubmed
  72. Kuroda Kawaguchi T, Skaletsky H, Brown L, Minx P, Cordum H, Waterston R, et al. The AZFc region of the Y chromosome features massive palindromes and uniform recurrent deletions in infertile men. Nat Genet. 2001;29:279-86 pubmed
    ..Deletions of AZFc that cause infertility are remarkably uniform, spanning a 3.5-Mb segment and bounded by 229-kb direct repeats that probably served as substrates for homologous recombination. ..
  73. Bradley J, Baltus A, Skaletsky H, Royce Tolland M, Dewar K, Page D. An X-to-autosome retrogene is required for spermatogenesis in mice. Nat Genet. 2004;36:872-6 pubmed
    ..This recurrence implies a strong selective pressure, perhaps to enable ribosome assembly in male meiotic cells. ..
  74. Xie X, Lu J, Kulbokas E, Golub T, Mootha V, Lindblad Toh K, et al. Systematic discovery of regulatory motifs in human promoters and 3' UTRs by comparison of several mammals. Nature. 2005;434:338-45 pubmed
    ..The overall results provide a systematic view of gene regulation in the human, which will be refined as additional mammalian genomes become available. ..
  75. Elbaz J, Yin P, Voigt C. Genetic encoding of DNA nanostructures and their self-assembly in living bacteria. Nat Commun. 2016;7:11179 pubmed publisher
    ..Genetically encoding DNA nanostructures provides a route for their production as well as applications in living cells. ..
  76. Sloma A, Rufo G, Theriault K, Dwyer M, Wilson S, Pero J. Cloning and characterization of the gene for an additional extracellular serine protease of Bacillus subtilis. J Bacteriol. 1991;173:6889-95 pubmed
    ..The vpr gene maps in the ctrA-sacA-epr region of the chromosome and is not required for growth or sporulation. ..
  77. Hallam S, Mincer T, Schleper C, Preston C, Roberts K, Richardson P, et al. Pathways of carbon assimilation and ammonia oxidation suggested by environmental genomic analyses of marine Crenarchaeota. PLoS Biol. 2006;4:e95 pubmed
    ..These findings further substantiate the likely global metabolic importance of Crenarchaeota with respect to key steps in the biogeochemical transformation of carbon and nitrogen in marine ecosystems...
  78. Pepinsky R, Silvian L, Berkowitz S, Farrington G, Lugovskoy A, Walus L, et al. Improving the solubility of anti-LINGO-1 monoclonal antibody Li33 by isotype switching and targeted mutagenesis. Protein Sci. 2010;19:954-66 pubmed publisher
    ..Through these efforts we were able to improve the solubility of the Li33 Mab from 0.3 mg/mL to >50 mg/mL and reduce aggregation to an acceptable level. These strategies can be readily applied to other proteins with solubility issues. ..
  79. Buhl L, Jorquera R, Akbergenova Y, Huntwork Rodriguez S, Volfson D, Littleton J. Differential regulation of evoked and spontaneous neurotransmitter release by C-terminal modifications of complexin. Mol Cell Neurosci. 2013;52:161-72 pubmed publisher
  80. Libermann T, Baltimore D. Activation of interleukin-6 gene expression through the NF-kappa B transcription factor. Mol Cell Biol. 1990;10:2327-34 pubmed
    ..Because NF-kappa B is involved in the control of a variety of genes activated upon inflammation, NF-kappa B may play a central role in the inflammatory response to infection and tissue injury. ..
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