Genomes and Genes
Experts and Doctors on molecular cloning in Boston, Massachusetts, United States
Locale: Boston, Massachusetts, United States
Topic: molecular cloning
- Lee S, Yu A, Lytton J. Tissue-specific expression of Na(+)-Ca2+ exchanger isoforms. J Biol Chem. 1994;269:14849-52 pubmed..Therefore, we propose that the structural complexity of the single NCE gene allows it to respond independently to the unique demands of different environments. ..
- Pohl U, Smith J, Tachibana I, Ueki K, Lee H, Ramaswamy S, et al. EHD2, EHD3, and EHD4 encode novel members of a highly conserved family of EH domain-containing proteins. Genomics. 2000;63:255-62 pubmed..Mutation analysis of the five coding exons of EHD2 in gliomas failed to detect any tumor-specific alterations, thus indicating that EHD2 is an unlikely candidate for the 19q tumor suppressor gene. ..
- Jain M, Fujita K, Hsieh C, Endege W, Sibinga N, Yet S, et al. Molecular cloning and characterization of SmLIM, a developmentally regulated LIM protein preferentially expressed in aortic smooth muscle cells. J Biol Chem. 1996;271:10194-9 pubmed..The down-regulation of SmLIM by phenotypic change in vascular smooth muscle cells suggests that it may be involved in their growth and differentiation. ..
- Yu H, King R, Peters J, Kirschner M. Identification of a novel ubiquitin-conjugating enzyme involved in mitotic cyclin degradation. Curr Biol. 1996;6:455-66 pubmed..However, as the patterns of conjugates generated by these E2s are distinct, these enzymes may play different roles in promoting cyclin proteolysis in mitosis. ..
- Bae D, Schaefer M, Partan B, Muglia L. Characterization of the mouse DAX-1 gene reveals evolutionary conservation of a unique amino-terminal motif and widespread expression in mouse tissue. Endocrinology. 1996;137:3921-7 pubmed..Future studies using mouse models of altered DAX-1 expression will be critical in defining the role of this factor in tissue- and development-specific gene regulation. ..
- Oettgen P, Akbarali Y, Boltax J, Best J, Kunsch C, Libermann T. Characterization of NERF, a novel transcription factor related to the Ets factor ELF-1. Mol Cell Biol. 1996;16:5091-106 pubmed..Since NERF and ELF-1 are coexpressed in B and T cells, both might be involved in the regulation of the same genes. ..
- Stukenberg P, Lustig K, McGarry T, King R, Kuang J, Kirschner M. Systematic identification of mitotic phosphoproteins. Curr Biol. 1997;7:338-48 pubmed
- Kumar S, Kao W, Howley P. Physical interaction between specific E2 and Hect E3 enzymes determines functional cooperativity. J Biol Chem. 1997;272:13548-54 pubmed
- Neufeld E, Skalnik D, Lievens P, Orkin S. Human CCAAT displacement protein is homologous to the Drosophila homeoprotein, cut. Nat Genet. 1992;1:50-5 pubmed..As cut participates in determination of cell fate in several tissues in Drosophila, the similarity predicts a broad role for CDP in mammalian development. ..
- Choi H, Chung M, Tzameli I, Simha D, Lee Y, Seol W, et al. Differential transactivation by two isoforms of the orphan nuclear hormone receptor CAR. J Biol Chem. 1997;272:23565-71 pubmed..As expected from its inability to bind DNA, the mCAR2 variant neither transactivates by itself nor inhibits transactivation by hCAR or mCAR1. ..
- Liang X, Jensen K, Frank Kamenetskii M. Very efficient template/primer-independent DNA synthesis by thermophilic DNA polymerase in the presence of a thermophilic restriction endonuclease. Biochemistry. 2004;43:13459-66 pubmed..A possible role of RE-pol DNA synthesis in the evolutionary development of genetic materials is briefly discussed. ..
- Hashimoto N, Feener E, Zhang W, Goldstein B. Insulin receptor protein-tyrosine phosphatases. Leukocyte common antigen-related phosphatase rapidly deactivates the insulin receptor kinase by preferential dephosphorylation of the receptor regulatory domain. J Biol Chem. 1992;267:13811-4 pubmed
- Tran P, Haycraft C, Besschetnova T, Turbe Doan A, Stottmann R, Herron B, et al. THM1 negatively modulates mouse sonic hedgehog signal transduction and affects retrograde intraflagellar transport in cilia. Nat Genet. 2008;40:403-410 pubmed publisher..Specifically, the aln mutation uncouples the roles of anterograde and retrograde transport in SHH signaling, suggesting that anterograde IFT is required for GLI activation and that retrograde IFT modulates this event. ..
- Chaillet J, Knoll J, Horsthemke B, Lalande M. The syntenic relationship between the critical deletion region for the Prader-Willi/Angelman syndromes and proximal mouse chromosome 7. Genomics. 1991;11:773-6 pubmed..Because the Prader-Willi and Angelman syndromes are postulated to result from genomic imprinting within the common deletion, these probes may define a genomically imprinted region on mouse chromosome 7. ..
- Cai G, Anumanthan A, Brown J, Greenfield E, Zhu B, Freeman G. CD160 inhibits activation of human CD4+ T cells through interaction with herpesvirus entry mediator. Nat Immunol. 2008;9:176-85 pubmed publisher..CD160 thus serves as a negative regulator of CD4+ T cell activation through its interaction with HVEM. ..
- Russo T, Thompson J, Godoy V, Malamy M. Cloning and expression of the Bacteroides fragilis TAL2480 neuraminidase gene, nanH, in Escherichia coli. J Bacteriol. 1990;172:2594-600 pubmed..We identified five regions showing great similarity to the Asp boxes, -Ser-X-Asp-X-Gly-X-Thr-Trp-, of other bacterial and viral NANase proteins. ..
- Rual J, Hirozane Kishikawa T, Hao T, Bertin N, Li S, Dricot A, et al. Human ORFeome version 1.1: a platform for reverse proteomics. Genome Res. 2004;14:2128-35 pubmed..1 represents a central resource for the cloning of large sets of human ORFs in various settings for functional proteomics of many types, and will serve as the foundation for subsequent improved versions of the human ORFeome. ..
- Kleijnen M, Alarcon R, Howley P. The ubiquitin-associated domain of hPLIC-2 interacts with the proteasome. Mol Biol Cell. 2003;14:3868-75 pubmed..It is possible that the function of these proteins may be regulated or mediated through proteasomal degradation pathways. ..
- Chen H, Thalmann I, Adams J, Avraham K, Copeland N, Jenkins N, et al. cDNA cloning, tissue distribution, and chromosomal localization of Ocp2, a gene encoding a putative transcription-associated factor predominantly expressed in the auditory organs. Genomics. 1995;27:389-98 pubmed publisher..The Ocp2 gene was mapped to mouse chromosome 4 as well as 11. Our results suggest that OCP-II may be involved in transcription regulation for the development or maintenance of specialized functions of the inner ear...
- Marigo V, Roberts D, Lee S, Tsukurov O, Levi T, Gastier J, et al. Cloning, expression, and chromosomal location of SHH and IHH: two human homologues of the Drosophila segment polarity gene hedgehog. Genomics. 1995;28:44-51 pubmed..Shh mapped to a position 0.6 cM distal to En2 and 1.9 cM proximal to Il6 on mouse chromosome 5. This location is closely linked but distinct from the murine limb mutation Hx and syntenic to human chromosome 7q36. ..
- Azim A, Knoll J, Beggs A, Chishti A. Isoform cloning, actin binding, and chromosomal localization of human erythroid dematin, a member of the villin superfamily. J Biol Chem. 1995;270:17407-13 pubmed..The dematin locus, 8p21.1, is distal to the known locus of human erythroid ankyrin (8p11.2) and may contribute to the etiology of hemolytic anemia in a subset of patients with severe hereditary spherocytosis. ..
- Pallas D, Weller W, Jaspers S, Miller T, Lane W, Roberts T. The third subunit of protein phosphatase 2A (PP2A), a 55-kilodalton protein which is apparently substituted for by T antigens in complexes with the 36- and 63-kilodalton PP2A subunits, bears little resemblance to T antigens. J Virol. 1992;66:886-93 pubmed..Somewhat surprisingly, there was no clear homology with T-antigen amino acid sequences. ..
- Ho K, Nichols C, Lederer W, Lytton J, Vassilev P, Kanazirska M, et al. Cloning and expression of an inwardly rectifying ATP-regulated potassium channel. Nature. 1993;362:31-8 pubmed..But the presence of an H5 region, which is likely to form the ion conduction pathway, indicates that the protein may share a common origin with voltage-gated potassium channel proteins. ..
- Dangond F, Hafler D, Tong J, Randall J, Kojima R, Utku N, et al. Differential display cloning of a novel human histone deacetylase (HDAC3) cDNA from PHA-activated immune cells. Biochem Biophys Res Commun. 1998;242:648-52 pubmed..Functional activity of the expressed HDAC3 protein was confirmed in alpha-HDAC3 antibody immunoprecipitates by a histone deacetylase assay. Our study suggests the participation of HDACs in cell cycle progression and activation. ..
- Kwon J, Keates S, Simeonidis S, Grall F, Libermann T, Keates A. ESE-1, an enterocyte-specific Ets transcription factor, regulates MIP-3alpha gene expression in Caco-2 human colonic epithelial cells. J Biol Chem. 2003;278:875-84 pubmed
- RayChaudhuri D, Park J. Escherichia coli cell-division gene ftsZ encodes a novel GTP-binding protein. Nature. 1992;359:251-4 pubmed..The membrane association of FtsZ and the known ability of GTPases to act as molecular switches implicate FtsZ in a GTP-activated signal transduction pathway that may regulate the start of septation in E. coli. ..
- Yergeau D, Hetherington C, Wang Q, Zhang P, Sharpe A, Binder M, et al. Embryonic lethality and impairment of haematopoiesis in mice heterozygous for an AML1-ETO fusion gene. Nat Genet. 1997;15:303-6 pubmed..This indicates that AML1-ETO may have other functions besides blocking wild-type AML1, a property that may be important in leukaemogenesis. ..
- Withers D, Gutierrez J, Towery H, Burks D, Ren J, Previs S, et al. Disruption of IRS-2 causes type 2 diabetes in mice. Nature. 1998;391:900-4 pubmed..Our results indicate that dysfunction of IRS-2 may contribute to the pathophysiology of human type 2 diabetes. ..
- Yelick P, Abduljabbar T, Stashenko P. zALK-8, a novel type I serine/threonine kinase receptor, is expressed throughout early zebrafish development. Dev Dyn. 1998;211:352-61 pubmed..3 kb. zALK-8 mRNA expression correlates well with known functions of TGF-beta family members as early axial patterning and mesoderm-inducing growth factors and as potent growth and differentiation factors in craniofacial development. ..
- Schwartz F, Eisenman R, Knoll J, Gessler M, Bruns G. cDNA sequence, genomic organization, and evolutionary conservation of a novel gene from the WAGR region. Genomics. 1995;29:526-32 pubmed..The extensive conservation of the predicted protein suggests a fundamental function of the gene product and will enable evaluation of the role of the 239FB gene in neurogenesis in model organisms...
- Bhattacharya S, Michels C, Leung M, Arany Z, Kung A, Livingston D. Functional role of p35srj, a novel p300/CBP binding protein, during transactivation by HIF-1. Genes Dev. 1999;13:64-75 pubmed
- Li Y, Chen W. Characterization of mouse cathepsin K gene, the gene promoter, and the gene expression. J Bone Miner Res. 1999;14:487-99 pubmed
- Bergemann A, Cheng H, Brambilla R, Klein R, Flanagan J. ELF-2, a new member of the Eph ligand family, is segmentally expressed in mouse embryos in the region of the hindbrain and newly forming somites. Mol Cell Biol. 1995;15:4921-9 pubmed..Comparable patterns have been described for Eph family receptors, including Sek-4 and Nuk/Sek-3, suggesting roles for ELF-2 in patterning these regions of the embryo. ..
- Fleming J, Steinkamp M, Kawatsuji R, Tartaglini E, Pinkus J, Pinkus G, et al. Characterization of a murine high-affinity thiamine transporter, Slc19a2. Mol Genet Metab. 2001;74:273-80 pubmed..Immunohistochemistry studies indicate that Slc19a2 is expressed on the cell surface and intracellularly, and is specifically localized to a subpopulation of cells in cochlea, small intestine, and pancreas. ..
- Shi J, Kandror K. Sortilin is essential and sufficient for the formation of Glut4 storage vesicles in 3T3-L1 adipocytes. Dev Cell. 2005;9:99-108 pubmed..Thus, sortilin is not only essential, but also sufficient for biogenesis of GSVs and acquisition of insulin responsiveness in adipose cells. ..
- Wong S, Carroll P, Rahme L, Ausubel F, Calderwood S. Modulation of expression of the ToxR regulon in Vibrio cholerae by a member of the two-component family of response regulators. Infect Immun. 1998;66:5854-61 pubmed..varA represents an additional modulating factor in the coordinate expression of virulence factors in V. cholerae...
- Shafman T, Khanna K, Kedar P, Spring K, Kozlov S, Yen T, et al. Interaction between ATM protein and c-Abl in response to DNA damage. Nature. 1997;387:520-3 pubmed..These findings indicate that ATM is involved in the activation of c-Abl by DNA damage and this interaction may in part mediate radiation-induced G1 arrest. ..
- Tsukaguchi H, Weremowicz S, Morton C, Hediger M. Functional and molecular characterization of the human neutral solute channel aquaporin-9. Am J Physiol. 1999;277:F685-96 pubmed..1-22.2. Our data show that hAQP9 serves as a promiscuous solute channel expressed in both liver and peripheral leukocytes, where it is ideally suited to transport of metabolites and/or nutrients into and out of these cells ..
- Rao V, Corradetti M, Chen J, Peng J, Yuan J, Prestwich G, et al. Expression cloning of protein targets for 3-phosphorylated phosphoinositides. J Biol Chem. 1999;274:37893-900 pubmed..This report demonstrates the utility of this technique for isolating and characterizing 3'-PPI-binding proteins and has broad applicability for the isolation of binding domains for other lipid products. ..
- Ahn A, Freener C, Gussoni E, Yoshida M, Ozawa E, Kunkel L. The three human syntrophin genes are expressed in diverse tissues, have distinct chromosomal locations, and each bind to dystrophin and its relatives. J Biol Chem. 1996;271:2724-30 pubmed
- David V, Hochstenbach F, Rajagopalan S, Brenner M. Interaction with newly synthesized and retained proteins in the endoplasmic reticulum suggests a chaperone function for human integral membrane protein IP90 (calnexin). J Biol Chem. 1993;268:9585-92 pubmed..Together, these data offer further evidence suggesting that IP90 may act in assisting protein assembly and/or in the retention within the ER of unassembled protein subunits. ..
- Lee Y, Beinborn M, McBride E, Lu M, Kolakowski L, Kopin A. The human brain cholecystokinin-B/gastrin receptor. Cloning and characterization. J Biol Chem. 1993;268:8164-9 pubmed..Our data suggest that the CCK-B and gastrin receptors are identical and that the long standing distinction between them may no longer apply. ..
- Kirchhausen T, Davis A, Frucht S, Greco B, Payne G, Tubb B. AP17 and AP19, the mammalian small chains of the clathrin-associated protein complexes show homology to Yap17p, their putative homolog in yeast. J Biol Chem. 1991;266:11153-7 pubmed..The yeast sequence predicts Yap17p, a protein with 147 amino acids and a Mr of 17,373 that is slightly more related to the mammalian AP17 chain than to its AP19 counterpart. ..
- Ibrahim E, Hims M, Shomron N, Burge C, Slaugenhaupt S, Reed R. Weak definition of IKBKAP exon 20 leads to aberrant splicing in familial dysautonomia. Hum Mutat. 2007;28:41-53 pubmed
- Musacchio M, Perrimon N. The Drosophila kekkon genes: novel members of both the leucine-rich repeat and immunoglobulin superfamilies expressed in the CNS. Dev Biol. 1996;178:63-76 pubmed..Genetic analysis reveals that deletion of the kek1 gene causes no obvious developmental defects. The coexpression of kek2 in the CNS leads us to suggest that Kek1 is part of a family of cell surface proteins with redundant function. ..
- Herrera V, Ponce L, Bagamasbad P, VanPelt B, Didishvili T, Ruiz Opazo N. Embryonic lethality in Dear gene-deficient mice: new player in angiogenesis. Physiol Genomics. 2005;23:257-68 pubmed
- Gerard N, Lu B, Liu P, Craig S, Fujiwara Y, Okinaga S, et al. An anti-inflammatory function for the complement anaphylatoxin C5a-binding protein, C5L2. J Biol Chem. 2005;280:39677-80 pubmed..Accordingly, up-regulation of C5L2 may be of benefit in inflammatory states driven by C5a, including sepsis, asthma, cystic fibrosis, and chronic obstructive lung disease. ..
- Reynolds D, Gurley D, Austen K, Serafin W. Cloning of the cDNA and gene of mouse mast cell protease-6. Transcription by progenitor mast cells and mast cells of the connective tissue subclass. J Biol Chem. 1991;266:3847-53 pubmed
- Fernandez C, Butterfield C, Jackson G, Moses M. Structural and functional uncoupling of the enzymatic and angiogenic inhibitory activities of tissue inhibitor of metalloproteinase-2 (TIMP-2): loop 6 is a novel angiogenesis inhibitor. J Biol Chem. 2003;278:40989-95 pubmed..In addition, we report, for the first time, the discovery of Loop 6 as a novel and potent inhibitor of angiogenesis. ..
- Naisbitt S, Valtschanoff J, Allison D, Sala C, Kim E, Craig A, et al. Interaction of the postsynaptic density-95/guanylate kinase domain-associated protein complex with a light chain of myosin-V and dynein. J Neurosci. 2000;20:4524-34 pubmed..We discuss the possibility that the GKAP/DLC interaction may be involved in trafficking of the PSD-95 complex by motor proteins. ..
- Wolf G, Trub T, Ottinger E, Groninga L, Lynch A, White M, et al. PTB domains of IRS-1 and Shc have distinct but overlapping binding specificities. J Biol Chem. 1995;270:27407-10 pubmed..Shc and IRS-1 are phosphorylated by distinct but overlapping sets of receptor-linked tyrosine kinases. These differences may be accounted for by the inherent specificities of their respective PTB domains. ..
- Takada Y, Hemler M. The primary structure of the VLA-2/collagen receptor alpha 2 subunit (platelet GPIa): homology to other integrins and the presence of a possible collagen-binding domain. J Cell Biol. 1989;109:397-407 pubmed..L. Strominger, and M. E. Hemler. 1987. Proc. Natl. Acad. Sci. USA. 84:3239-3243). Resolution of this discrepancy suggests that there may be another VLA heterodimer that resembles VLA-2 in size but has a different amino acid sequence. ..
- Logothetis D, Kammen B, Lindpaintner K, Bisbas D, Nadal Ginard B. Gating charge differences between two voltage-gated K+ channels are due to the specific charge content of their respective S4 regions. Neuron. 1993;10:1121-9 pubmed..However, differences in residues inside the S4 region are not sufficient to account for each channel's characteristic voltage range of activation. ..
- Mirzabekov T, Bannert N, Farzan M, Hofmann W, Kolchinsky P, Wu L, et al. Enhanced expression, native purification, and characterization of CCR5, a principal HIV-1 coreceptor. J Biol Chem. 1999;274:28745-50 pubmed..The methods described herein contribute to the analysis of CCR5 and are likely to be applicable to many other GPCRs. ..
- Veale M, Raab M, Li Z, da Silva A, Kraeft S, Weremowicz S, et al. Novel isoform of lymphoid adaptor FYN-T-binding protein (FYB-130) interacts with SLP-76 and up-regulates interleukin 2 production. J Biol Chem. 1999;274:28427-35 pubmed..1. FYB-130 therefore represents a novel variant of FYB protein that can up-regulate T-cell receptor-driven interleukin 2 production in mature T-cells. ..
- Zhang F, Lücke C, Baier L, Sacchettini J, Hamilton J. Solution structure of human intestinal fatty acid binding protein with a naturally-occurring single amino acid substitution (A54T) that is associated with altered lipid metabolism. Biochemistry. 2003;42:7339-47 pubmed..Structural details of this portal region will be discussed in view of the influence residue 54 exerts on the functional properties of human I-FABP. ..
- Chen X, Rubock M, Whitman M. A transcriptional partner for MAD proteins in TGF-beta signalling. Nature. 1996;383:691-6 pubmed..A model is proposed to explain how TGF-beta superfamily signals might regulate the expression of specific genes in the early embryo. ..
- Wong K, Cantley L. Cloning and characterization of a human phosphatidylinositol 4-kinase. J Biol Chem. 1994;269:28878-84 pubmed
- Qiu X, Lin Y, Thome K, Pian P, Schlegel B, Weremowicz S, et al. An eukaryotic RuvB-like protein (RUVBL1) essential for growth. J Biol Chem. 1998;273:27786-93 pubmed..Yeast with a null mutation in scRUVBL1 was nonviable. Thus RUVBL1 is an eukaryotic member of the RuvB/clamp loader family of structurally related proteins from bacteria and eukaryotes that is essential for viability of yeast. ..
- Perraud A, Fleig A, Dunn C, Bagley L, Launay P, Schmitz C, et al. ADP-ribose gating of the calcium-permeable LTRPC2 channel revealed by Nudix motif homology. Nature. 2001;411:595-9 pubmed..These results indicate that intracellular ADPR regulates calcium entry into cells that express LTRPC2. ..
- Larson R, Corbi A, Berman L, Springer T. Primary structure of the leukocyte function-associated molecule-1 alpha subunit: an integrin with an embedded domain defining a protein superfamily. J Cell Biol. 1989;108:703-12 pubmed..These structural features indicate a bipartite evolution from the integrin family and from an I domain family. These features may also correspond to relevant functional domains. ..
- Dykxhoorn D, Wu Y, Xie H, Yu F, Lal A, Petrocca F, et al. miR-200 enhances mouse breast cancer cell colonization to form distant metastases. PLoS ONE. 2009;4:e7181 pubmed publisher..These results suggest that for some tumors, tumor colonization at metastatic sites might be enhanced by MET. Therefore the epithelial nature of a tumor does not predict metastatic outcome. ..
- Salehi Ashtiani K, Yang X, Derti A, Tian W, Hao T, Lin C, et al. Isoform discovery by targeted cloning, 'deep-well' pooling and parallel sequencing. Nat Methods. 2008;5:597-600 pubmed publisher..This ORFeome discovery pipeline will be applicable to any eukaryotic species with a sequenced genome. ..
- Bloch K, Hong C, Eddy R, Shows T, Quertermous T. cDNA cloning and chromosomal assignment of the endothelin 2 gene: vasoactive intestinal contractor peptide is rat endothelin 2. Genomics. 1991;10:236-42 pubmed..The endothelin peptide encoded by the rat gene differed from ET2 at 1 of 21 residues and was identical to mouse VIC. We conclude that VIC is the mouse and rat analogue of the human ET2 gene. ..
- Kong T, Westerman K, Faigle M, Eltzschig H, Colgan S. HIF-dependent induction of adenosine A2B receptor in hypoxia. FASEB J. 2006;20:2242-50 pubmed..These findings may provide an important link between hypoxia and metabolic conditions associated with inflammation and angiogenesis. ..
- Vig M, Beck A, Billingsley J, Lis A, Parvez S, Peinelt C, et al. CRACM1 multimers form the ion-selective pore of the CRAC channel. Curr Biol. 2006;16:2073-9 pubmed..Our data provide unequivocal evidence that CRACM1 multimers form the Ca(2+)-selective CRAC-channel pore. ..
- Bastos R, Ribas de Pouplana L, Enarson M, Bodoor K, Burke B. Nup84, a novel nucleoporin that is associated with CAN/Nup214 on the cytoplasmic face of the nuclear pore complex. J Cell Biol. 1997;137:989-1000 pubmed..In this way, Nup84 could play a central role in the organization of the interface between the pore complex and the cytoplasm. ..
- Tolias K, Bikoff J, Burette A, Paradis S, Harrar D, Tavazoie S, et al. The Rac1-GEF Tiam1 couples the NMDA receptor to the activity-dependent development of dendritic arbors and spines. Neuron. 2005;45:525-38 pubmed..Taken together, these findings define a molecular mechanism by which NMDA receptor signaling controls the growth and morphology of dendritic arbors and spines. ..
- Wang Y, El Zaru M, Surks H, Mendelsohn M. Formin homology domain protein (FHOD1) is a cyclic GMP-dependent protein kinase I-binding protein and substrate in vascular smooth muscle cells. J Biol Chem. 2004;279:24420-6 pubmed..Based on the known functions of FHOD1, the data are consistent with a role for FHOD1 in cyclic GMP-dependent inhibition of VSMC stress fiber formation and/or migration. ..
- Shaw S, Cepek K, Murphy E, Russell G, Brenner M, Parker C. Molecular cloning of the human mucosal lymphocyte integrin alpha E subunit. Unusual structure and restricted RNA distribution. J Biol Chem. 1994;269:6016-25 pubmed..High levels of alpha E and beta 7 mRNA were restricted to mucosal lymphocytes supporting the hypothesis that alpha E beta 7 plays a role in the localization or site-specific functions of intra-epithelial T-lymphocytes. ..
- Ren D, Navarro B, Perez G, Jackson A, Hsu S, Shi Q, et al. A sperm ion channel required for sperm motility and male fertility. Nature. 2001;413:603-9 pubmed..CatSper is thus vital to cAMP-mediated Ca2+ influx in sperm, sperm motility and fertilization. CatSper represents an excellent target for non-hormonal contraceptives for both men and women. ..
- Melville S, Labbe R, Sonenshein A. Expression from the Clostridium perfringens cpe promoter in C. perfringens and Bacillus subtilis. Infect Immun. 1994;62:5550-8 pubmed..In B. subtilis, however, the 5' end was internal to the 45-bp insertion, suggesting the use of a different promoter than that utilized by C. perfringens...
- Layne M, Yet S, Maemura K, Hsieh C, Bernfield M, Perrella M, et al. Impaired abdominal wall development and deficient wound healing in mice lacking aortic carboxypeptidase-like protein. Mol Cell Biol. 2001;21:5256-61 pubmed..5-day-postconception embryos exhibited a reduced proliferative capacity compared with wild-type cells. These results indicate that ACLP is an ECM protein that is essential for embryonic development and dermal wound healing processes. ..
- Mosialos G, Hanissian S, Jawahar S, Vara L, Kieff E, Chatila T. A Ca2+/calmodulin-dependent protein kinase, CaM kinase-Gr, expressed after transformation of primary human B lymphocytes by Epstein-Barr virus (EBV) is induced by the EBV oncogene LMP1. J Virol. 1994;68:1697-705 pubmed..In view of the key role played by LMP1 in B-lymphocyte immortalization by EBV, these findings implicate CaM kinase-Gr as a potential mediator of B-lymphocyte growth transformation. ..
- Li Y, Alexander M, Wucherpfennig A, Yelick P, Chen W, Stashenko P. Cloning and complete coding sequence of a novel human cathepsin expressed in giant cells of osteoclastomas. J Bone Miner Res. 1995;10:1197-202 pubmed..These data strongly support the hypothesis that cathepsin X represents a novel cysteine proteinase which is expressed at high levels in osteoclasts. ..
- Hsieh C, Yoshizumi M, Endege W, Kho C, Jain M, Kashiki S, et al. APEG-1, a novel gene preferentially expressed in aortic smooth muscle cells, is down-regulated by vascular injury. J Biol Chem. 1996;271:17354-9 pubmed..Taken together, these data indicate that APEG-1 is a novel marker for differentiated ASMC and may have a role in regulating growth and differentiation of this cell type. ..
- Brooks C, Scherer P, Cleveland K, Whittemore J, Lodish H, Cheatham B. Pantophysin is a phosphoprotein component of adipocyte transport vesicles and associates with GLUT4-containing vesicles. J Biol Chem. 2000;275:2029-36 pubmed..In addition, pantophysin may provide a marker for the analysis of other vesicles in adipocytes. ..
- Patterson C, Perrella M, Hsieh C, Yoshizumi M, Lee M, Haber E. Cloning and functional analysis of the promoter for KDR/flk-1, a receptor for vascular endothelial growth factor. J Biol Chem. 1995;270:23111-8 pubmed..Understanding the KDR/flk-1 promoter will allow us to investigate endothelial cell-specific gene regulation and to uncover methods for targeting gene delivery specifically to endothelial cells. ..
- Pfleger C, Salic A, Lee E, Kirschner M. Inhibition of Cdh1-APC by the MAD2-related protein MAD2L2: a novel mechanism for regulating Cdh1. Genes Dev. 2001;15:1759-64 pubmed..MAD2L2 specifically binds and inhibits Cdh1-APC, paralleling the effect of MAD2 on Cdc20. We suggest that MAD2L2 and MAD2 inhibit the release of substrates from APC and propose a mechanism of inhibition. ..
- Jin Y, Burakoff S, Bierer B. Molecular cloning of a 25-kDa high affinity rapamycin binding protein, FKBP25. J Biol Chem. 1992;267:10942-5 pubmed..These data demonstrate that FKBP25 has a higher affinity for rapamycin than for FK506 and suggest that this cellular receptor may be an important target molecule for immunosuppression by rapamycin. ..
- Pollak M, Brown E, Chou Y, Hebert S, Marx S, Steinmann B, et al. Mutations in the human Ca(2+)-sensing receptor gene cause familial hypocalciuric hypercalcemia and neonatal severe hyperparathyroidism. Cell. 1993;75:1297-303 pubmed..We conclude that the mammalian Ca(2+)-sensing receptor "sets" the extracellular Ca2+ level and is defective in individuals with FHH and NSHPT. ..
- Apte S, Mattei M, Olsen B. Cloning of the cDNA encoding human tissue inhibitor of metalloproteinases-3 (TIMP-3) and mapping of the TIMP3 gene to chromosome 22. Genomics. 1994;19:86-90 pubmed..Using in situ hybridization to human metaphase chromosomes, we have assigned the locus for the TIMP3 gene to the q12.1-q13.2 region of human chromosome 22. ..