Experts and Doctors on iron sulfur proteins in Boston, Massachusetts, United States


Locale: Boston, Massachusetts, United States
Topic: iron sulfur proteins

Top Publications

  1. Hidalgo E, Demple B. Activation of SoxR-dependent transcription in vitro by noncatalytic or NifS-mediated assembly of [2Fe-2S] clusters into apo-SoxR. J Biol Chem. 1996;271:7269-72 pubmed
    ..Catalytic generation of [2Fe-2S]-containing SoxR could allow for rapid activation of this transcription factor in vivo. ..
  2. Ding H, Hidalgo E, Demple B. The redox state of the [2Fe-2S] clusters in SoxR protein regulates its activity as a transcription factor. J Biol Chem. 1996;271:33173-5 pubmed
    ..The results suggest that one-electron oxidation and reduction of the [2Fe-2S] cluster regulate SoxR transcriptional activity. ..
  3. Wingert R, Galloway J, Barut B, Foott H, Fraenkel P, Axe J, et al. Deficiency of glutaredoxin 5 reveals Fe-S clusters are required for vertebrate haem synthesis. Nature. 2005;436:1035-39 pubmed
    ..These findings uncover a connection between haem biosynthesis and Fe-S clusters, indicating that haemoglobin production in the differentiating red cell is regulated through Fe-S cluster assembly. ..
  4. Shaw G, Cope J, Li L, Corson K, Hersey C, Ackermann G, et al. Mitoferrin is essential for erythroid iron assimilation. Nature. 2006;440:96-100 pubmed
    ..Our data show that mfrn functions as the principal mitochondrial iron importer essential for haem biosynthesis in vertebrate erythroblasts...
  5. Bak D, Zuris J, Paddock M, Jennings P, Elliott S. Redox characterization of the FeS protein MitoNEET and impact of thiazolidinedione drug binding. Biochemistry. 2009;48:10193-5 pubmed publisher
    ..In contrast, a His87Cys mutant negates the ability of TZDs to affect the midpoint potential, suggesting a model of drug binding in which His87 is critical to communication with the FeS center of mitoNEET. ..
  6. Xiao Y, Zahariou G, Sanakis Y, Liu P. IspG enzyme activity in the deoxyxylulose phosphate pathway: roles of the iron-sulfur cluster. Biochemistry. 2009;48:10483-5 pubmed publisher
    ..In addition, we have improved the Escherichia coli IspG activity to 550 nmol min(-1) mg(-1), which is approximately 20-fold greater than that of the NADPH-Fpr-FldA system in the literature. ..
  7. Hidalgo E, Bollinger J, Bradley T, Walsh C, Demple B. Binuclear [2Fe-2S] clusters in the Escherichia coli SoxR protein and role of the metal centers in transcription. J Biol Chem. 1995;270:20908-14 pubmed
    ..However, lowering the thiol concentration by dilution allowed spontaneous regeneration of active Fe-SoxR. ..
  8. Iuchi S, Lin E. Adaptation of Escherichia coli to redox environments by gene expression. Mol Microbiol. 1993;9:9-15 pubmed
    ..In addition, the regulator FhlA is involved in a cascade-controlled pathway for the formate branch of the pyruvate fermentation pathway. ..
  9. Membrillo Hernandez J, Lin E. Regulation of expression of the adhE gene, encoding ethanol oxidoreductase in Escherichia coli: transcription from a downstream promoter and regulation by fnr and RpoS. J Bacteriol. 1999;181:7571-9 pubmed
    ..The results support the previously postulated ribosomal binding site (RBS) occlusion model, according to which RNase III cleavage is required to release the RBS from a stem-loop structure in the long transcript. ..

More Information


  1. Mitra S, Elliott S. Oxidative disassembly of the [2Fe-2S] cluster of human Grx2 and redox regulation in the mitochondria. Biochemistry. 2009;48:3813-5 pubmed publisher
    ..We find that the cluster signal is stable at positive potentials up to 0.5 V but that cluster destruction occurs readily when oxidative pulses in excess of this value are applied. ..