Genomes and Genes
Experts and Doctors on saccharomyces cerevisiae proteins in Bethesda, Maryland, United States
Locale: Bethesda, Maryland, United States
Topic: saccharomyces cerevisiae proteins
Publications150 found, 100 shown here
- Tjandra N, Suzuki M, Chang S. Refinement of protein structure against non-redundant carbonyl 13C NMR relaxation. J Biomol NMR. 2007;38:243-53 pubmed..In addition, possible variations of the CSA tensor were addressed. ..
- Kryndushkin D, Shewmaker F, Wickner R. Curing of the [URE3] prion by Btn2p, a Batten disease-related protein. EMBO J. 2008;27:2725-35 pubmed publisher..Btn2p curing requires cell division, and our results suggest that Btn2p is part of a system, reminiscent of the mammalian aggresome, that collects aggregates preventing their efficient distribution to progeny cells. ..
- Singh R, Brooks B, Klauda J. Binding and release of cholesterol in the Osh4 protein of yeast. Proteins. 2009;75:468-77 pubmed publisher..The mechanism of sterol release is conceptualized as a molecular ladder with the rungs being amino acids or water-mediated amino acids that interact with 3-OH. ..
- Wollert T, Wunder C, Lippincott Schwartz J, Hurley J. Membrane scission by the ESCRT-III complex. Nature. 2009;458:172-7 pubmed publisher..The minimum set of ESCRT-III and Vps4 proteins capable of multiple cycles of vesicle detachment corresponds to the ancient set of ESCRT proteins conserved from archaea to animals. ..
- Cole H, Howard B, Clark D. The centromeric nucleosome of budding yeast is perfectly positioned and covers the entire centromere. Proc Natl Acad Sci U S A. 2011;108:12687-92 pubmed publisher..The fact that the binding sites for Cbf1 and CBF3 are included within the centromeric nucleosome has important implications for models of the centromeric nucleosome and for kinetochore function. ..
- Anwar K, Klemm R, Condon A, Severin K, Zhang M, Ghirlando R, et al. The dynamin-like GTPase Sey1p mediates homotypic ER fusion in S. cerevisiae. J Cell Biol. 2012;197:209-17 pubmed publisher..Collectively, our results show that Sey1p and its homologues function analogously to ATLs in mediating ER fusion. They also indicate that S. cerevisiae has an alternative fusion mechanism that requires ER SNAREs. ..
- Brinkmann U, Brinkmann E, Gallo M, Pastan I. Cloning and characterization of a cellular apoptosis susceptibility gene, the human homologue to the yeast chromosome segregation gene CSE1. Proc Natl Acad Sci U S A. 1995;92:10427-31 pubmed..Thus, CAS appears to play an important role in both toxin and tumor necrosis factor-mediated cell death, as well as in cell proliferation. ..
- Wang Y, Santini F, Qin K, Huang C. A Mg(2+)-dependent, Ca(2+)-inhibitable serine/threonine protein phosphatase from bovine brain. J Biol Chem. 1995;270:25607-12 pubmed
- Burbelo P, Utani A, Pan Z, Yamada Y. Cloning of the large subunit of activator 1 (replication factor C) reveals homology with bacterial DNA ligases. Proc Natl Acad Sci U S A. 1993;90:11543-7 pubmed..The finding that the region required for the binding of murine A1 p145 to DNA has similarity to a domain found in DNA ligases suggests that this region may be utilized by both proteins in recognizing DNA. ..
- Jung G, Jones G, Wegrzyn R, Masison D. A role for cytosolic hsp70 in yeast [PSI(+)] prion propagation and [PSI(+)] as a cellular stress. Genetics. 2000;156:559-70 pubmed
- Strunnikov A, Aravind L, Koonin E. Saccharomyces cerevisiae SMT4 encodes an evolutionarily conserved protease with a role in chromosome condensation regulation. Genetics. 2001;158:95-107 pubmed..The SIZ1 gene disruption is synthetically lethal with the SIZ2 deletion. We propose that SMT4, SIZ1, and SIZ2 are involved in a novel pathway of chromosome maintenance. ..
- Prag G, Misra S, Jones E, Ghirlando R, Davies B, Horazdovsky B, et al. Mechanism of ubiquitin recognition by the CUE domain of Vps9p. Cell. 2003;113:609-20 pubmed..Dimerization of the CUE domain allows both surfaces to contact a single ubiquitin molecule, providing a mechanism for high-affinity binding to monoubiquitin. ..
- Liu S, Leppla S. Retroviral insertional mutagenesis identifies a small protein required for synthesis of diphthamide, the target of bacterial ADP-ribosylating toxins. Mol Cell. 2003;12:603-13 pubmed..KTI11, the analog of DESR1 in yeast, which was originally identified as a gene regulating the sensitivity of yeast to zymocin, is also required for diphthamide biosynthesis, implicating DESR1/KTI11 in multiple biological processes. ..
- Shakoury Elizeh M, Tiedeman J, Rashford J, Ferea T, Demeter J, Garcia E, et al. Transcriptional remodeling in response to iron deprivation in Saccharomyces cerevisiae. Mol Biol Cell. 2004;15:1233-43 pubmed..We provide evidence that yeast subjected to iron deprivation undergo a transcriptional remodeling, resulting in a shift from iron-dependent to parallel, but iron-independent, metabolic pathways. ..
- Thakurta A, Gopal G, Yoon J, Kozak L, Dhar R. Homolog of BRCA2-interacting Dss1p and Uap56p link Mlo3p and Rae1p for mRNA export in fission yeast. EMBO J. 2005;24:2512-23 pubmed..Thus, Dss1p may function at multiple steps of mRNA export, from mRNP biogenesis to their targeting and translocation through the NPC. ..
- Brachmann A, Baxa U, Wickner R. Prion generation in vitro: amyloid of Ure2p is infectious. EMBO J. 2005;24:3082-92 pubmed..Our data indicate that there is no substantial difference between filaments formed in vivo and in vitro. ..
- Jothi R, Cherukuri P, Tasneem A, Przytycka T. Co-evolutionary analysis of domains in interacting proteins reveals insights into domain-domain interactions mediating protein-protein interactions. J Mol Biol. 2006;362:861-75 pubmed
- Fekete C, Mitchell S, Cherkasova V, Applefield D, Algire M, Maag D, et al. N- and C-terminal residues of eIF1A have opposing effects on the fidelity of start codon selection. EMBO J. 2007;26:1602-14 pubmed..We conclude that tight binding of eIF1A to the PIC is an important determinant of AUG selection and is modulated in opposite directions by residues in the NTT and CTT of eIF1A. ..
- Mukhopadhyay D, Dasso M. Modification in reverse: the SUMO proteases. Trends Biochem Sci. 2007;32:286-95 pubmed..The dissimilar sub-nuclear localization patterns of Ulp/SENPs and phenotypes of Ulp/SENP mutants further indicate that different Ulp/SENPs have distinct and non-redundant roles. ..
- Wickner R, Shewmaker F, Kryndushkin D, Edskes H. Protein inheritance (prions) based on parallel in-register beta-sheet amyloid structures. Bioessays. 2008;30:955-64 pubmed publisher..This property of self-reproduction, in turn, allows these proteins to act as de facto genes, encoding heritable information. ..
- Luk E, Ranjan A, FitzGerald P, Mizuguchi G, Huang Y, Wei D, et al. Stepwise histone replacement by SWR1 requires dual activation with histone H2A.Z and canonical nucleosome. Cell. 2010;143:725-36 pubmed publisher..Z-H2B. These results suggest that the combination of H2A-containing nucleosome and free H2A.Z-H2B dimer acting as both effector and substrate for SWR1 governs the specificity and outcome of the replacement reaction. ..
- Mollapour M, Tsutsumi S, Truman A, Xu W, Vaughan C, Beebe K, et al. Threonine 22 phosphorylation attenuates Hsp90 interaction with cochaperones and affects its chaperone activity. Mol Cell. 2011;41:672-81 pubmed publisher..Overexpression of Aha1 stimulates the ATPase activity, restores cochaperone interactions, and compensates for the functional defects of these Hsp90 mutants. ..
- Ragusa M, Stanley R, Hurley J. Architecture of the Atg17 complex as a scaffold for autophagosome biogenesis. Cell. 2012;151:1501-1512 pubmed publisher..The C-terminal EAT domain of Atg1 was shown to sense membrane curvature, dimerize, and tether lipid vesicles. These data suggest a structural mechanism for the organization of Atg9 vesicles into the early phagophore...
- Jao C, Ragusa M, Stanley R, Hurley J. A HORMA domain in Atg13 mediates PI 3-kinase recruitment in autophagy. Proc Natl Acad Sci U S A. 2013;110:5486-91 pubmed publisher..These two Arg residues are essential for autophagy, suggesting that the Atg13 HORMA domain could function as a phosphoregulated conformational switch. ..
- Chereji R, Ocampo J, Clark D. MNase-Sensitive Complexes in Yeast: Nucleosomes and Non-histone Barriers. Mol Cell. 2017;65:565-577.e3 pubmed publisher..Thus, histone ChIP-seq experiments are essential to distinguish nucleosomes from other DNA-binding proteins that protect against MNase. ..
- Philpott C, Rashford J, Yamaguchi Iwai Y, Rouault T, Dancis A, Klausner R. Cell-cycle arrest and inhibition of G1 cyclin translation by iron in AFT1-1(up) yeast. EMBO J. 1998;17:5026-36 pubmed..Instead, this reduction occurs at the level of Cln2 translation, a recently recognized locus of cell-cycle control in yeast. ..
- Philpott C, Protchenko O, Kim Y, Boretsky Y, Shakoury Elizeh M. The response to iron deprivation in Saccharomyces cerevisiae: expression of siderophore-based systems of iron uptake. Biochem Soc Trans. 2002;30:698-702 pubmed..The Arn transporters are expressed in intracellular vesicles that correspond to the endosomal compartment, which suggests that intracellular trafficking of the siderophore and/or its transporter may be important for uptake. ..
- Protchenko O, Shakoury Elizeh M, Keane P, Storey J, Androphy R, Philpott C. Role of PUG1 in inducible porphyrin and heme transport in Saccharomyces cerevisiae. Eukaryot Cell. 2008;7:859-71 pubmed publisher..The data from these experiments were consistent with a role for Pug1p in inducible protoporphyrin IX influx and heme efflux. ..
- Hong J, Feng H, Wang F, Ranjan A, Chen J, Jiang J, et al. The catalytic subunit of the SWR1 remodeler is a histone chaperone for the H2A.Z-H2B dimer. Mol Cell. 2014;53:498-505 pubmed publisher..Z. Our results show that the Swr1-Z domain can deliver the H2A.Z-H2B dimer to the DNA-(H3-H4)2 tetrasome to form the nucleosome by a histone chaperone mechanism. ..
- Song Y, Wu Y, Jung G, Tutar Y, Eisenberg E, Greene L, et al. Role for Hsp70 chaperone in Saccharomyces cerevisiae prion seed replication. Eukaryot Cell. 2005;4:289-97 pubmed..Our data suggest that Ssa1-21p interferes with disruption of large Sup35p aggregates, which lack or have limited capacity to function as seed, into polymers that function more efficiently as [PSI+] seeds. ..
- Dong J, Nanda J, Rahman H, Pruitt M, Shin B, Wong C, et al. Genetic identification of yeast 18S rRNA residues required for efficient recruitment of initiator tRNA(Met) and AUG selection. Genes Dev. 2008;22:2242-55 pubmed publisher..Hence, the nonconserved 928:1389 base pair in h28, plus conserved 18S rRNA residues corresponding to P-site contacts in bacterial ribosomes, are critical for efficient Met-tRNA(i)(Met) binding and AUG selection in eukaryotes. ..
- Zhang K, Fischer T, Porter R, Dhakshnamoorthy J, Zofall M, Zhou M, et al. Clr4/Suv39 and RNA quality control factors cooperate to trigger RNAi and suppress antisense RNA. Science. 2011;331:1624-7 pubmed publisher..These findings link Clr4 to RNA quality control machinery and suggest a pathway for processing potentially deleterious RNAs through the coordinated actions of RNAi and other RNA processing activities. ..
- Pierce M, Maddelein M, Roberts B, Wickner R. A novel Rtg2p activity regulates nitrogen catabolism in yeast. Proc Natl Acad Sci U S A. 2001;98:13213-8 pubmed..These characteristics suggest that Rtg2p acts in the upstream part of the nitrogen catabolism regulation pathway. ..
- Lipman D, Souvorov A, Koonin E, Panchenko A, Tatusova T. The relationship of protein conservation and sequence length. BMC Evol Biol. 2002;2:20 pubmed..For all the organisms studied, there seems to be a significant evolutionary trend favoring shorter proteins in the absence of other, more specific functional constraints. ..
- Im Y, Hurley J. Integrated structural model and membrane targeting mechanism of the human ESCRT-II complex. Dev Cell. 2008;14:902-13 pubmed publisher..ESCRT-II is targeted to endosomal membranes by the lipid-binding activities of both the Vps36 GLUE domain and the first helix of Vps22. These data provide a unifying structural and functional framework for the ESCRT-II complex. ..
- Chon H, Sparks J, Rychlik M, Nowotny M, Burgers P, Crouch R, et al. RNase H2 roles in genome integrity revealed by unlinking its activities. Nucleic Acids Res. 2013;41:3130-43 pubmed publisher..However, this AGS-related mutation accumulates 2-5 bp deletions at a very similar rate as the deletion strain. ..
- Chantalat S, Park S, Hua Z, Liu K, Gobin R, Peyroche A, et al. The Arf activator Gea2p and the P-type ATPase Drs2p interact at the Golgi in Saccharomyces cerevisiae. J Cell Sci. 2004;117:711-22 pubmed..We characterize one such mutant that has a thermosensitive phenotype, and show that it has morphological defects along the secretory pathway in the formation of secretory granules/vesicles. ..
- Babu M, Iyer L, Balaji S, Aravind L. The natural history of the WRKY-GCM1 zinc fingers and the relationship between transcription factors and transposons. Nucleic Acids Res. 2006;34:6505-20 pubmed..We also use the WRKY-GCM1 superfamily as an example to illustrate the importance of transposons in the emergence of new TFs in different lineages. ..
- Jothi R, Przytycka T, Aravind L. Discovering functional linkages and uncharacterized cellular pathways using phylogenetic profile comparisons: a comprehensive assessment. BMC Bioinformatics. 2007;8:173 pubmed
- Speransky V, Taylor K, Edskes H, Wickner R, Steven A. Prion filament networks in [URE3] cells of Saccharomyces cerevisiae. J Cell Biol. 2001;153:1327-36 pubmed..These observations support and further specify the concept of the [URE3] prion as a self-propagating amyloid. ..
- Ichida M, Nemoto S, Finkel T. Identification of a specific molecular repressor of the peroxisome proliferator-activated receptor gamma Coactivator-1 alpha (PGC-1alpha). J Biol Chem. 2002;277:50991-5 pubmed..In addition, our results suggest that other co-activators might also have specific repressors, thereby identifying another layer of combinatorial complexity in transcriptional regulation. ..
- Sattlegger E, Hinnebusch A. Separate domains in GCN1 for binding protein kinase GCN2 and ribosomes are required for GCN2 activation in amino acid-starved cells. EMBO J. 2000;19:6622-33 pubmed..GCN1 expression conferred sensitivity to paromomycin in a manner dependent on its ribosome binding domain, supporting the idea that GCN1 binds near the ribosomal acceptor site to promote GCN2 activation by uncharged tRNA. ..
- Motegi A, Sood R, Moinova H, Markowitz S, Liu P, Myung K. Human SHPRH suppresses genomic instability through proliferating cell nuclear antigen polyubiquitination. J Cell Biol. 2006;175:703-8 pubmed..Therefore, the yRad5/SHPRH-dependent pathway is a conserved and fundamental DNA repair mechanism that protects the genome from genotoxic stress. ..
- Xiao H, Mizuguchi G, Wisniewski J, Huang Y, Wei D, Wu C. Nonhistone Scm3 binds to AT-rich DNA to organize atypical centromeric nucleosome of budding yeast. Mol Cell. 2011;43:369-80 pubmed publisher..These findings suggest a model in which centromere-bound Scm3 aids recruitment of Cse4/H4 to assemble and maintain an H2A/H2B-deficient centromeric nucleosome. ..
- Caplan S, Hartnell L, Aguilar R, Naslavsky N, Bonifacino J. Human Vam6p promotes lysosome clustering and fusion in vivo. J Cell Biol. 2001;154:109-22 pubmed..This study implicates hVam6p as a mammalian tethering/docking factor characterized with intrinsic ability to promote lysosome fusion in vivo. ..
- Banerjee S, Smith S, Oum J, Liaw H, Hwang J, Sikdar N, et al. Mph1p promotes gross chromosomal rearrangement through partial inhibition of homologous recombination. J Cell Biol. 2008;181:1083-93 pubmed publisher..Furthermore, spontaneous RPA foci at DSBs are destabilized by the mph1Delta mutation. Therefore, Mph1p promotes GCR formation by partially suppressing HR, likely through its interaction with RPA. ..
- Woodford M, Truman A, Dunn D, Jensen S, Cotran R, Bullard R, et al. Mps1 Mediated Phosphorylation of Hsp90 Confers Renal Cell Carcinoma Sensitivity and Selectivity to Hsp90 Inhibitors. Cell Rep. 2016;14:872-884 pubmed publisher..Mps1 expression level can potentially serve as a predictive indicator of tumor response to Hsp90 inhibitors. ..
- Iyer L, Aravind L, Bork P, Hofmann K, Mushegian A, Zhulin I, et al. Quod erat demonstrandum? The mystery of experimental validation of apparently erroneous computational analyses of protein sequences. Genome Biol. 2001;2:RESEARCH0051 pubmed..Some of these experiments might signify discovery of extremely unusual forms of the respective enzymes, whereas the results of others could be due to artifacts. ..
- Nielsen K, VALASEK L, Sykes C, Jivotovskaya A, Hinnebusch A. Interaction of the RNP1 motif in PRT1 with HCR1 promotes 40S binding of eukaryotic initiation factor 3 in yeast. Mol Cell Biol. 2006;26:2984-98 pubmed..Thus, the PRT1 RNP1 motif coordinates the functions of HCR1 and TIF32 in 40S binding of eIF3 and is needed for optimal preinitiation complex assembly and AUG recognition in vivo. ..
- Wickner R, Edskes H, Kryndushkin D, McGlinchey R, Bateman D, Kelly A. Prion diseases of yeast: amyloid structure and biology. Semin Cell Dev Biol. 2011;22:469-75 pubmed publisher..Moreover, we have pointed out that this amyloid architecture can explain how one protein can faithfully transmit any of several conformations to new protein monomers. This explains how proteins can be genes. ..
- Anderson J, Phan L, Hinnebusch A. The Gcd10p/Gcd14p complex is the essential two-subunit tRNA(1-methyladenosine) methyltransferase of Saccharomyces cerevisiae. Proc Natl Acad Sci U S A. 2000;97:5173-8 pubmed..Our results provide a demonstration of a two-component tRNA MTase and suggest that binding of AdoMet and tRNA substrates depends on different subunits of the complex. ..
- Kim Y, Yun C, Philpott C. Ferrichrome induces endosome to plasma membrane cycling of the ferrichrome transporter, Arn1p, in Saccharomyces cerevisiae. EMBO J. 2002;21:3632-42 pubmed..Thus, siderophores influence the trafficking of the Arn transporters within the cell and this trafficking is important for transporter function...
- Lee J, Pestova T, Shin B, Cao C, Choi S, Dever T. Initiation factor eIF5B catalyzes second GTP-dependent step in eukaryotic translation initiation. Proc Natl Acad Sci U S A. 2002;99:16689-94 pubmed..Thus, in contrast to bacteria where the single GTPase IF2 is sufficient to catalyze translation initiation, eukaryotic cells require hydrolysis of GTP by both eIF2 and eIF5B to complete translation initiation. ..
- Jeong H, Backlund P, CHEN H, Karavanov A, Crouch R. RNase H2 of Saccharomyces cerevisiae is a complex of three proteins. Nucleic Acids Res. 2004;32:407-14 pubmed..The multisubunit nature of S.cerevisiae RNase H2 may be important both for structural purposes and to provide a means of interacting with other proteins involved in DNA replication/repair and transcription. ..
- Chiu W, Wagner S, HerrmannovÃ¡ A, Burela L, Zhang F, Saini A, et al. The C-terminal region of eukaryotic translation initiation factor 3a (eIF3a) promotes mRNA recruitment, scanning, and, together with eIF3j and the eIF3b RNA recognition motif, selection of AUG start codons. Mol Cell Biol. 2010;30:4415-34 pubmed publisher
- Cole H, Howard B, Clark D. Activation-induced disruption of nucleosome position clusters on the coding regions of Gcn4-dependent genes extends into neighbouring genes. Nucleic Acids Res. 2011;39:9521-35 pubmed publisher..We conclude that activation results in gene-wide chromatin remodelling and that this remodelling can even extend into the chromatin of flanking genes. ..
- Cui F, Cole H, Clark D, Zhurkin V. Transcriptional activation of yeast genes disrupts intragenic nucleosome phasing. Nucleic Acids Res. 2012;40:10753-64 pubmed publisher..This unusual pattern suggests that the +1 nucleosomes might be prone to sliding, thereby facilitating transcription. ..
- Huang Y, Intine R, Mozlin A, Hasson S, Maraia R. Mutations in the RNA polymerase III subunit Rpc11p that decrease RNA 3' cleavage activity increase 3'-terminal oligo(U) length and La-dependent tRNA processing. Mol Cell Biol. 2005;25:621-36 pubmed..The data indicate that Rpc11p limits RNA 3'-U length and that this significantly restricts pre-tRNAs to a La-independent pathway of maturation in fission yeast. ..
- Qiu H, Hu C, Zhang F, Hwang G, Swanson M, Boonchird C, et al. Interdependent recruitment of SAGA and Srb mediator by transcriptional activator Gcn4p. Mol Cell Biol. 2005;25:3461-74 pubmed..Thus, while Tra1p can bind directly to Gcn4p in vitro, it requires other SAGA subunits for efficient recruitment in vivo. ..
- Hinnebusch A, Asano K, Olsen D, Phan L, Nielsen K, VALASEK L. Study of translational control of eukaryotic gene expression using yeast. Ann N Y Acad Sci. 2004;1038:60-74 pubmed..Thus, apart from its critical role in the starvation response, GCN4 regulation is a valuable tool for dissecting the contributions of multiple translation factors in the eukaryotic initiation pathway. ..
- Kim Y, McLaughlin N, Lindstrom K, Tsukiyama T, Clark D. Activation of Saccharomyces cerevisiae HIS3 results in Gcn4p-dependent, SWI/SNF-dependent mobilization of nucleosomes over the entire gene. Mol Cell Biol. 2006;26:8607-22 pubmed..We propose that Gcn4p stimulates nucleosome mobilization over the entire HIS3 gene by the SWI/SNF complex. We suggest that the net effect of interplay among remodeling machines at HIS3 is to create a highly dynamic chromatin structure. ..
- Zhou Z, Feng H, Zhou B, Ghirlando R, Hu K, Zwolak A, et al. Structural basis for recognition of centromere histone variant CenH3 by the chaperone Scm3. Nature. 2011;472:234-7 pubmed publisher..Scm3(CBD) induces major conformational changes and sterically occludes DNA-binding sites in the structure of Cse4 and H4. These findings have implications for the assembly and architecture of the centromeric nucleosome. ..
- Shin B, Kim J, Walker S, Dong J, Lorsch J, Dever T. Initiation factor eIF2Î³ promotes eIF2-GTP-Met-tRNAi(Met) ternary complex binding to the 40S ribosome. Nat Struct Mol Biol. 2011;18:1227-34 pubmed publisher..Thus, despite their structural similarity, eIF2 and EF-Tu bind tRNAs in substantially different manners, and we propose that the tRNA-binding domain III of EF-Tu has acquired a new ribosome-binding function in eIF2Î³. ..
- Kryndushkin D, Ihrke G, Piermartiri T, Shewmaker F. A yeast model of optineurin proteinopathy reveals a unique aggregation pattern associated with cellular toxicity. Mol Microbiol. 2012;86:1531-47 pubmed publisher..This study generates a mechanistic framework for investigating how OPTN may trigger pathological changes in ALS and other OPTN-linked neurodegenerative disorders. ..
- Monaghan E, Gable K, Dunn T. Mutations in the Lcb2p subunit of serine palmitoyltransferase eliminate the requirement for the TSC3 gene in Saccharomyces cerevisiae. Yeast. 2002;19:659-70 pubmed
- Sakchaisri K, Asano S, Yu L, Shulewitz M, Park C, Park J, et al. Coupling morphogenesis to mitotic entry. Proc Natl Acad Sci U S A. 2004;101:4124-9 pubmed..This mechanism links assembly of a cellular structure to passage into mitosis. ..
- Fekete C, Applefield D, Blakely S, Shirokikh N, Pestova T, Lorsch J, et al. The eIF1A C-terminal domain promotes initiation complex assembly, scanning and AUG selection in vivo. EMBO J. 2005;24:3588-601 pubmed..Thus, the OB-fold is crucial for ribosome-binding and the C-terminal domain of eIF1A has eukaryotic-specific functions in TC recruitment and scanning. ..
- Mendiratta G, Eriksson P, Clark D. Cooperative binding of the yeast Spt10p activator to the histone upstream activating sequences is mediated through an N-terminal dimerization domain. Nucleic Acids Res. 2007;35:812-21 pubmed..Residues 1-396 are sufficient for normal growth, indicating that the critical functions of Spt10p reside in the N-terminal domains. ..
- Wickner R, Shewmaker F, Edskes H, Kryndushkin D, Nemecek J, McGlinchey R, et al. Prion amyloid structure explains templating: how proteins can be genes. FEMS Yeast Res. 2010;10:980-91 pubmed publisher..The prion domain sequences generally vary more rapidly in evolution than does the remainder of the molecule, producing a barrier to prion transmission, perhaps selected in evolution by this protection. ..
- VALASEK L, Mathew A, Shin B, Nielsen K, Szamecz B, Hinnebusch A. The yeast eIF3 subunits TIF32/a, NIP1/c, and eIF5 make critical connections with the 40S ribosome in vivo. Genes Dev. 2003;17:786-99 pubmed
- Govind C, Qiu H, Ginsburg D, Ruan C, Hofmeyer K, Hu C, et al. Phosphorylated Pol II CTD recruits multiple HDACs, including Rpd3C(S), for methylation-dependent deacetylation of ORF nucleosomes. Mol Cell. 2010;39:234-46 pubmed publisher..A strong correlation between increased acetylation and lower histone occupancy in HDA mutants implies that histone acetylation is important for nucleosome eviction. ..
- Gutierrez E, Shin B, Woolstenhulme C, Kim J, Saini P, Buskirk A, et al. eIF5A promotes translation of polyproline motifs. Mol Cell. 2013;51:35-45 pubmed publisher..Thus, eIF5A, like its bacterial ortholog EFP, is proposed to stimulate the peptidyl transferase activity of the ribosome and facilitate the reactivity of poor substrates like Pro...
- Ross K, COHEN FIX O. A role for the FEAR pathway in nuclear positioning during anaphase. Dev Cell. 2004;6:729-35 pubmed..We propose that at anaphase onset, the FEAR pathway activates cytoplasmic microtubule-associated forces that facilitate chromosome segregation to the mother cell. ..
- Kostelansky M, Sun J, Lee S, Kim J, Ghirlando R, Hierro A, et al. Structural and functional organization of the ESCRT-I trafficking complex. Cell. 2006;125:113-26 pubmed..The C-terminal domain of Vps28 binds the ESCRT-II complex. The structure shows how ESCRT-I is assembled by a compact core from which the Vps23 UEV domain, the Vps28 C domain, and other domains project to bind their partners. ..
- Schulz T, Choi M, Raychaudhuri S, Mears J, Ghirlando R, Hinshaw J, et al. Lipid-regulated sterol transfer between closely apposed membranes by oxysterol-binding protein homologues. J Cell Biol. 2009;187:889-903 pubmed publisher..Together, these findings suggest a new model of how ORPs could sense and regulate the lipid composition of adjacent membranes. ..
- Wickner R, Edskes H, Shewmaker F, Kryndushkin D, Nemecek J, McGlinchey R, et al. The relationship of prions and translation. Wiley Interdiscip Rev RNA. 2010;1:81-9 pubmed publisher..Another connection of prions with translation is the influence on prion propagation and generation of ribosome-associated chaperones, the Ssbs, and a chaperone activity intrinsic to the 60S ribosomal subunits. ..
- Ainsworth W, Hughes B, Au W, Sakelaris S, Kerscher O, Benton M, et al. Cytoplasmic localization of Hug1p, a negative regulator of the MEC1 pathway, coincides with the compartmentalization of Rnr2p-Rnr4p. Biochem Biophys Res Commun. 2013;439:443-8 pubmed publisher..Taken together, the genetic interactions, gene expression, and localization studies support a novel role for Hug1p as a negative regulator of the MEC1 checkpoint response through its compartmentalization with Rnr2p-Rnr4p. ..
- Wisniewski J, Orosz A, Allada R, Wu C. The C-terminal region of Drosophila heat shock factor (HSF) contains a constitutively functional transactivation domain. Nucleic Acids Res. 1996;24:367-74 pubmed..The Drosophila and human HSF transactivation domains are both rich in hydrophobic and acidic residues and may be structurally conserved, despite limited sequence identity. ..
- Qiang Y, Endo Y, Rubin J, Rudikoff S. Wnt signaling in B-cell neoplasia. Oncogene. 2003;22:1536-45 pubmed
- Yoon S, Govind C, Qiu H, Kim S, Dong J, Hinnebusch A. Recruitment of the ArgR/Mcm1p repressor is stimulated by the activator Gcn4p: a self-checking activation mechanism. Proc Natl Acad Sci U S A. 2004;101:11713-8 pubmed..By recruiting an arginine-regulated repressor, Gcn4p can precisely modulate its activation function at ARG1 according to the availability of arginine. ..
- VALASEK L, Nielsen K, Zhang F, Fekete C, Hinnebusch A. Interactions of eukaryotic translation initiation factor 3 (eIF3) subunit NIP1/c with eIF1 and eIF5 promote preinitiation complex assembly and regulate start codon selection. Mol Cell Biol. 2004;24:9437-55 pubmed..Thus, the NIP1-NTD is required for efficient assembly of preinitiation complexes and also regulates the selection of AUG start codons in vivo. ..