Genomes and Genes
Experts and Doctors on saccharomyces cerevisiae in Bethesda, Maryland, United States
Locale: Bethesda, Maryland, United States
Topic: saccharomyces cerevisiae
Publications148 found, 100 shown here
- Kryndushkin D, Wickner R. Nucleotide exchange factors for Hsp70s are required for [URE3] prion propagation in Saccharomyces cerevisiae. Mol Biol Cell. 2007;18:2149-54 pubmed..Sse1p and Fes1p are nucleotide exchange factors for Ssa1p. Interestingly, deletion of either SSE1 or FES1 completely blocked [URE3] propagation. In addition, deletion of SSE1 also interfered with [PSI(+)] propagation. ..
- Cheng T, Hu X, Gnatt A, Brooks P. Differential blocking effects of the acetaldehyde-derived DNA lesion N2-ethyl-2'-deoxyguanosine on transcription by multisubunit and single subunit RNA polymerases. J Biol Chem. 2008;283:27820-8 pubmed publisher..We also include models of the N(2)-Et-dG within the active site of yeast RNAPII, which are compatible with our observations. ..
- McCue P, Phang J. Identification of human intracellular targets of the medicinal Herb St. John's Wort by chemical-genetic profiling in yeast. J Agric Food Chem. 2008;56:11011-7 pubmed publisher..The comprehensive and systematic nature of chemical-genetic profiling in yeast makes this technique attractive for elucidating the potential molecular mechanisms of action of botanical medicines and other bioactive dietary plants. ..
- McGlinchey R, Kryndushkin D, Wickner R. Suicidal [PSI+] is a lethal yeast prion. Proc Natl Acad Sci U S A. 2011;108:5337-41 pubmed publisher..Our findings give a more realistic picture of the impact of the prion change than does focus on "mild" prion variants. ..
- Ren X, Hurley J. Structural basis for endosomal recruitment of ESCRT-I by ESCRT-0 in yeast. EMBO J. 2011;30:2130-9 pubmed publisher..However, this site is non-essential for sorting of the ESCRT cargo Cps1. Taken together, these results show how a conserved motif/domain pair can evolve to use strikingly different binding modes in different organisms. ..
- De Muyt A, Jessop L, Kolar E, Sourirajan A, Chen J, Dayani Y, et al. BLM helicase ortholog Sgs1 is a central regulator of meiotic recombination intermediate metabolism. Mol Cell. 2012;46:43-53 pubmed publisher..We conclude that Sgs1 is a primary regulator of recombination pathway choice during meiosis and suggest a similar function in the mitotic cell cycle. ..
- Xiang X. Nuclear positioning: dynein needed for microtubule shrinkage-coupled movement. Curr Biol. 2012;22:R496-9 pubmed publisher..A new study has revealed a novel protein interaction linking microtubule plus-ends with the cortex and a role for dynein in microtubule shrinkage-coupled movement. ..
- Ragusa M, Stanley R, Hurley J. Architecture of the Atg17 complex as a scaffold for autophagosome biogenesis. Cell. 2012;151:1501-1512 pubmed publisher..The C-terminal EAT domain of Atg1 was shown to sense membrane curvature, dimerize, and tether lipid vesicles. These data suggest a structural mechanism for the organization of Atg9 vesicles into the early phagophore...
- Arimbasseri A, Kassavetis G, Maraia R. Transcription. Comment on "Mechanism of eukaryotic RNA polymerase III transcription termination". Science. 2014;345:524 pubmed publisher..We could not corroborate their findings using bona fide Pol III from two laboratory sources. We show that Pol III efficiently terminates transcription in the absence of a hairpin/duplex in vitro and in vivo. ..
- Tutar Y, Song Y, Masison D. Primate chaperones Hsc70 (constitutive) and Hsp70 (induced) differ functionally in supporting growth and prion propagation in Saccharomyces cerevisiae. Genetics. 2006;172:851-61 pubmed..Our data support a view that intrinsic activities of Hsp70 isoforms are comparable, and functional differences in vivo lie mainly in complex interactions of Hsp70 with cochaperones. ..
- Boeckmann L, Takahashi Y, Au W, Mishra P, Choy J, Dawson A, et al. Phosphorylation of centromeric histone H3 variant regulates chromosome segregation in Saccharomyces cerevisiae. Mol Biol Cell. 2013;24:2034-44 pubmed publisher..Taken together, our results provide a detailed analysis, in vivo and in vitro, of Cse4 phosphorylation and its role in promoting faithful chromosome segregation. ..
- Raben N, Exelbert R, Spiegel R, Sherman J, Nakajima H, Plotz P, et al. Functional expression of human mutant phosphofructokinase in yeast: genetic defects in French Canadian and Swiss patients with phosphofructokinase deficiency. Am J Hum Genet. 1995;56:131-41 pubmed..The mutants expressed in yeast generate functional enzyme with modest changes in thermal stability. The advantages and limitations of the yeast system for expression of human mutant PFKs are discussed...
- Choi S, Lee J, Zoll W, Merrick W, Dever T. Promotion of met-tRNAiMet binding to ribosomes by yIF2, a bacterial IF2 homolog in yeast. Science. 1998;280:1757-60 pubmed..Thus, the mechanism of protein synthesis in eukaryotes and prokaryotes is more similar than was previously realized...
- Luk E, Vu N, Patteson K, Mizuguchi G, Wu W, Ranjan A, et al. Chz1, a nuclear chaperone for histone H2AZ. Mol Cell. 2007;25:357-68 pubmed..The presence of this motif in other metazoan proteins suggests that H2AZ-specific chaperones may be widely conserved. ..
- Qiu H, Hu C, Hinnebusch A. Phosphorylation of the Pol II CTD by KIN28 enhances BUR1/BUR2 recruitment and Ser2 CTD phosphorylation near promoters. Mol Cell. 2009;33:752-62 pubmed publisher..By contrast, CTK1 is responsible for the bulk of Ser2P in total Pol II and at promoter-distal sites. In addition to phosphorylating Ser2 near promoters, BUR1/BUR2 also stimulates Ser2P formation by CTK1 during transcription elongation. ..
- Chon H, Sparks J, Rychlik M, Nowotny M, Burgers P, Crouch R, et al. RNase H2 roles in genome integrity revealed by unlinking its activities. Nucleic Acids Res. 2013;41:3130-43 pubmed publisher..However, this AGS-related mutation accumulates 2-5 bp deletions at a very similar rate as the deletion strain. ..
- Vermut M, Widner W, Dinman J, Wickner R. Sequence of MKT1, needed for propagation of M2 satellite dsRNA of the L-A virus of Saccharomyces cerevisiae. Yeast. 1994;10:1477-9 pubmed..We report that MKT1 encodes a 92,979 Da protein with serine-rich regions and the retroviral protease signature, DTG, but with no substantial homology to proteins presently in the databases. ..
- Ichida M, Nemoto S, Finkel T. Identification of a specific molecular repressor of the peroxisome proliferator-activated receptor gamma Coactivator-1 alpha (PGC-1alpha). J Biol Chem. 2002;277:50991-5 pubmed..In addition, our results suggest that other co-activators might also have specific repressors, thereby identifying another layer of combinatorial complexity in transcriptional regulation. ..
- Kerscher O, Crotti L, Basrai M. Recognizing chromosomes in trouble: association of the spindle checkpoint protein Bub3p with altered kinetochores and a unique defective centromere. Mol Cell Biol. 2003;23:6406-18 pubmed..Our studies represent the first comprehensive molecular analysis of spindle checkpoint protein function in the context of a wild-type or defective kinetochore(s) by use of live-cell imaging and the ChIP technique in S. cerevisiae. ..
- Crotti L, Basrai M. Functional roles for evolutionarily conserved Spt4p at centromeres and heterochromatin in Saccharomyces cerevisiae. EMBO J. 2004;23:1804-14 pubmed..Our results highlight the evolutionary conservation of pathways required for genome stability in yeast and humans. ..
- Ross E, Edskes H, Terry M, Wickner R. Primary sequence independence for prion formation. Proc Natl Acad Sci U S A. 2005;102:12825-30 pubmed..These results suggest that [PSI+] formation is driven primarily by the amino acid composition of the Sup35p prion domain, and that the Sup35p oligopeptide repeats are not required for prion maintenance. ..
- Mollapour M, Tsutsumi S, Truman A, Xu W, Vaughan C, Beebe K, et al. Threonine 22 phosphorylation attenuates Hsp90 interaction with cochaperones and affects its chaperone activity. Mol Cell. 2011;41:672-81 pubmed publisher..Overexpression of Aha1 stimulates the ATPase activity, restores cochaperone interactions, and compensates for the functional defects of these Hsp90 mutants. ..
- Kryndushkin D, Wickner R, Tycko R. The core of Ure2p prion fibrils is formed by the N-terminal segment in a parallel cross-Î² structure: evidence from solid-state NMR. J Mol Biol. 2011;409:263-77 pubmed publisher..Finally, we show that a model in which CTD units are attached rigidly to the parallel Î²-sheet core is consistent with steric constraints. ..
- Xiao H, Mizuguchi G, Wisniewski J, Huang Y, Wei D, Wu C. Nonhistone Scm3 binds to AT-rich DNA to organize atypical centromeric nucleosome of budding yeast. Mol Cell. 2011;43:369-80 pubmed publisher..These findings suggest a model in which centromere-bound Scm3 aids recruitment of Cse4/H4 to assemble and maintain an H2A/H2B-deficient centromeric nucleosome. ..
- Toulmay A, Prinz W. A conserved membrane-binding domain targets proteins to organelle contact sites. J Cell Sci. 2012;125:49-58 pubmed publisher..The SMP domain binds membranes and is necessary for protein targeting to MCSs. Proteins containing this domain could be involved in lipid metabolism. This is the first protein domain found exclusively in proteins at MCSs. ..
- Woodford M, Truman A, Dunn D, Jensen S, Cotran R, Bullard R, et al. Mps1 Mediated Phosphorylation of Hsp90 Confers Renal Cell Carcinoma Sensitivity and Selectivity to Hsp90 Inhibitors. Cell Rep. 2016;14:872-884 pubmed publisher..Mps1 expression level can potentially serve as a predictive indicator of tumor response to Hsp90 inhibitors. ..
- Pouliot J, Robertson C, Nash H. Pathways for repair of topoisomerase I covalent complexes in Saccharomyces cerevisiae. Genes Cells. 2001;6:677-87 pubmed..The efficiency of these pathways differs for complexes induced with the chemotherapeutic agent camptothecin vs. those accumulated by mutant forms of topoisomerase I. ..
- Takahashi Y, Dulev S, Liu X, Hiller N, Zhao X, Strunnikov A. Cooperation of sumoylated chromosomal proteins in rDNA maintenance. PLoS Genet. 2008;4:e1000215 pubmed publisher..In addition, binding of cohesin and condensin to rDNA is altered in the mms21-CH E3-deficient mutant. ..
- Ren X, Hurley J. VHS domains of ESCRT-0 cooperate in high-avidity binding to polyubiquitinated cargo. EMBO J. 2010;29:1045-54 pubmed publisher..Mutational analysis of all the five ubiquitin-binding sites in yeast ESCRT-0 shows that cooperation between them is required for the sorting of the Lys63-linked polyubiquitinated cargo Cps1 to the vacuole. ..
- Philpott C, Leidgens S, Frey A. Metabolic remodeling in iron-deficient fungi. Biochim Biophys Acta. 2012;1823:1509-20 pubmed publisher..However, the regulatory mechanisms that accomplish these adaptations differ greatly between fungal species. This article is part of a Special Issue entitled: Cell Biology of Metals. ..
- Kimura S, Smith H, Hankinson O, Nebert D. Analysis of two benzo[a]pyrene-resistant mutants of the mouse hepatoma Hepa-1 P(1)450 gene via cDNA expression in yeast. EMBO J. 1987;6:1929-33 pubmed..This alteration in a single amino acid from arginine to proline might block electron flow directly, or change secondary structure of the protein, such that normal monooxygenation of benzo[a]pyrene cannot occur. ..
- Neuwald A. A shared domain between a spindle assembly checkpoint protein and Ypt/Rab-specific GTPase-activators. Trends Biochem Sci. 1997;22:243-4 pubmed
- McDonald J, Tissier A, Frank E, Iwai S, Hanaoka F, Woodgate R. DNA polymerase iota and related rad30-like enzymes. Philos Trans R Soc Lond B Biol Sci. 2001;356:53-60 pubmed..Based upon in vitro studies, it appears that Pol iota has the lowest fidelity of any eukaryotic polymerase studied to date and we speculate as to the possible cellular functions of such a remarkably error-prone DNA polymerase. ..
- Shakoury Elizeh M, Tiedeman J, Rashford J, Ferea T, Demeter J, Garcia E, et al. Transcriptional remodeling in response to iron deprivation in Saccharomyces cerevisiae. Mol Biol Cell. 2004;15:1233-43 pubmed..We provide evidence that yeast subjected to iron deprivation undergo a transcriptional remodeling, resulting in a shift from iron-dependent to parallel, but iron-independent, metabolic pathways. ..
- Stojmirović A, Yu Y. Information flow in interaction networks II: channels, path lengths, and potentials. J Comput Biol. 2012;19:379-403 pubmed publisher..Through examples involving the yeast pheromone response pathway, we illustrate the versatility and stability of our new framework. ..
- Ohno H, Stewart J, Fournier M, Bosshart H, Rhee I, Miyatake S, et al. Interaction of tyrosine-based sorting signals with clathrin-associated proteins. Science. 1995;269:1872-5 pubmed..The interaction was confirmed by in vitro binding assays. Thus, it is likely that the medium chains serve as signal-binding components of the clathrin-dependent sorting machinery. ..
- Wolffe A. Histone deacetylase: a regulator of transcription. Science. 1996;272:371-2 pubmed
- Ganguly S, Mummaneni P, Steinbach P, Klein D, Coon S. Characterization of the Saccharomyces cerevisiae homolog of the melatonin rhythm enzyme arylalkylamine N-acetyltransferase (EC 184.108.40.206). J Biol Chem. 2001;276:47239-47 pubmed..Perhaps the most striking difference is that fungal AANATs lack the regulatory domains of the vertebrate enzyme, which appear to be essential for the regulatory role the enzyme plays in photochemical transduction. ..
- Babenko V, Krylov D. Comparative analysis of complete genomes reveals gene loss, acquisition and acceleration of evolutionary rates in Metazoa, suggests a prevalence of evolution via gene acquisition and indicates that the evolutionary rates in animals tend to be conserv. Nucleic Acids Res. 2004;32:5029-35 pubmed..Finally, we show that the rate of evolution of a gene family in animals has a clear, but not absolute, tendency to be conserved. ..
- Khan M, Chock P, Stadtman E. Knockout of caspase-like gene, YCA1, abrogates apoptosis and elevates oxidized proteins in Saccharomyces cerevisiae. Proc Natl Acad Sci U S A. 2005;102:17326-31 pubmed..These findings suggest that deletion of the apoptotic-related caspase-like gene leads to a large H(2)O(2)-dependent accumulation of oxidized proteins and up-regulation of 20S proteasome activity. ..
- Karpova T, Kim M, Spriet C, Nalley K, Stasevich T, Kherrouche Z, et al. Concurrent fast and slow cycling of a transcriptional activator at an endogenous promoter. Science. 2008;319:466-9 pubmed publisher..These findings counter the prevailing view that slow cycling initiates transcription. ..
- Kryndushkin D, Wickner R, Shewmaker F. FUS/TLS forms cytoplasmic aggregates, inhibits cell growth and interacts with TDP-43 in a yeast model of amyotrophic lateral sclerosis. Protein Cell. 2011;2:223-36 pubmed publisher..Moreover, the biophysical properties of FUS aggregates in yeast are distinctly different from many amyloidogenic proteins, suggesting they are not composed of amyloid. ..
- Wickner R, Edskes H, Bateman D, Kelly A, Gorkovskiy A. The yeast prions [PSI+] and [URE3] are molecular degenerative diseases. Prion. 2011;5:258-62 pubmed publisher..Recently, we showed that the array of [PSI] and [URE3] prions includes a majority of lethal or very toxic variants, a result not expected if either prion were an adaptive cellular response to stress. ..
- Rojas M, Gingras A, Dever T. Protein phosphatase PP1/GLC7 interaction domain in yeast eIF2? bypasses targeting subunit requirement for eIF2? dephosphorylation. Proc Natl Acad Sci U S A. 2014;111:E1344-53 pubmed publisher..Taken together, these results indicate that, in contrast to the paradigm of distinct PP1-targeting or regulatory subunits, the unique N terminus of yeast eIF2? functions in cis to target GLC7 to dephosphorylate eIF2?. ..
- Olsen D, Savner E, Mathew A, Zhang F, Krishnamoorthy T, Phan L, et al. Domains of eIF1A that mediate binding to eIF2, eIF3 and eIF5B and promote ternary complex recruitment in vivo. EMBO J. 2003;22:193-204 pubmed..We propose a modular organization for eIF1A wherein a core ribosome-binding domain is flanked by flexible segments that mediate interactions with other factors involved in recruitment of TC and release of eIF1A at subunit joining. ..
- Chattopadhyay M, Tabor C, Tabor H. Spermidine but not spermine is essential for hypusine biosynthesis and growth in Saccharomyces cerevisiae: spermine is converted to spermidine in vivo by the FMS1-amine oxidase. Proc Natl Acad Sci U S A. 2003;100:13869-74 pubmed..We have also shown this conversion in enzymatic studies by using the purified amine oxidase from yeast. ..
- Wagner W, Hammer J. Myosin V and the endoplasmic reticulum: the connection grows. J Cell Biol. 2003;163:1193-6 pubmed..Together with other studies in plant and animal cells, the results of Estrada et al. (2003) support the idea that myosin V acts as a universal motor for the transport of ER membranes. ..
- VALASEK L, Nielsen K, Zhang F, Fekete C, Hinnebusch A. Interactions of eukaryotic translation initiation factor 3 (eIF3) subunit NIP1/c with eIF1 and eIF5 promote preinitiation complex assembly and regulate start codon selection. Mol Cell Biol. 2004;24:9437-55 pubmed..Thus, the NIP1-NTD is required for efficient assembly of preinitiation complexes and also regulates the selection of AUG start codons in vivo. ..
- Dev K, Qiu H, Dong J, Zhang F, Barthlme D, Hinnebusch A. The beta/Gcd7 subunit of eukaryotic translation initiation factor 2B (eIF2B), a guanine nucleotide exchange factor, is crucial for binding eIF2 in vivo. Mol Cell Biol. 2010;30:5218-33 pubmed publisher..In aggregate, these findings provide compelling evidence that ?/Gcd7 is crucial for binding of substrate by eIF2B in vivo, beyond its dispensable regulatory role in the inhibition of eIF2B by eIF (?P). ..
- Chereji R, Ocampo J, Clark D. MNase-Sensitive Complexes in Yeast: Nucleosomes and Non-histone Barriers. Mol Cell. 2017;65:565-577.e3 pubmed publisher..Thus, histone ChIP-seq experiments are essential to distinguish nucleosomes from other DNA-binding proteins that protect against MNase. ..
- Xu C. cDNA cloning of a mouse factor that activates transcription from a metal response element of the mouse metallothionein-I gene in yeast. DNA Cell Biol. 1993;12:517-25 pubmed..The cDNA hybridizes to a 550-base mRNA that is constitutively expressed in mouse tissue culture cells. The ability of the mouse factor to activate transcription in yeast cells is dependent upon the carbon source. ..
- Dell Angelica E, Puertollano R, Mullins C, Aguilar R, Vargas J, Hartnell L, et al. GGAs: a family of ADP ribosylation factor-binding proteins related to adaptors and associated with the Golgi complex. J Cell Biol. 2000;149:81-94 pubmed..Disruption of both GGA genes in yeast resulted in impaired trafficking of carboxypeptidase Y to the vacuole. These observations suggest that GGAs are components of ARF-regulated coats that mediate protein trafficking at the TGN. ..
- Seo E, Liu F, Kawagishi Kobayashi M, Ung T, Cao C, Dar A, et al. Protein kinase PKR mutants resistant to the poxvirus pseudosubstrate K3L protein. Proc Natl Acad Sci U S A. 2008;105:16894-9 pubmed publisher..We propose that these paradoxical effects of the PKR mutations on pseudosubstrate vs. substrate interactions reflect differences between the rigid K3L protein and the plastic nature of eIF2alpha around the Ser-51 phosphorylation site...
- Cui F, Cole H, Clark D, Zhurkin V. Transcriptional activation of yeast genes disrupts intragenic nucleosome phasing. Nucleic Acids Res. 2012;40:10753-64 pubmed publisher..This unusual pattern suggests that the +1 nucleosomes might be prone to sliding, thereby facilitating transcription. ..
- Tatusov R, Altschul S, Koonin E. Detection of conserved segments in proteins: iterative scanning of sequence databases with alignment blocks. Proc Natl Acad Sci U S A. 1994;91:12091-5 pubmed..The procedure described was used to detect novel conserved motifs of potential biological importance. ..
- Janvier K, Kato Y, Boehm M, Rose J, Martina J, Kim B, et al. Recognition of dileucine-based sorting signals from HIV-1 Nef and LIMP-II by the AP-1 gamma-sigma1 and AP-3 delta-sigma3 hemicomplexes. J Cell Biol. 2003;163:1281-90 pubmed..These observations reveal a novel mode of recognition of sorting signals involving the gamma/delta and sigma subunits of AP-1 and AP-3. ..
- Nielsen K, VALASEK L, Sykes C, Jivotovskaya A, Hinnebusch A. Interaction of the RNP1 motif in PRT1 with HCR1 promotes 40S binding of eukaryotic initiation factor 3 in yeast. Mol Cell Biol. 2006;26:2984-98 pubmed..Thus, the PRT1 RNP1 motif coordinates the functions of HCR1 and TIF32 in 40S binding of eIF3 and is needed for optimal preinitiation complex assembly and AUG recognition in vivo. ..
- Qiu H, Hu C, Wong C, Hinnebusch A. The Spt4p subunit of yeast DSIF stimulates association of the Paf1 complex with elongating RNA polymerase II. Mol Cell Biol. 2006;26:3135-48 pubmed..spt4Delta reduces trimethylation of Lys4 on histone H3, demonstrating a new role for yeast DSIF in promoting a Paf1C-dependent function in elongation. ..
- Bonifacino J, Dell Angelica E. Immunoprecipitation. Curr Protoc Cell Biol. 2001;Chapter 7:Unit 7.2 pubmed publisher..An additional method is provided for increasing the specificity of the technique by reprecipitating the antigen with the same or a different antibody. ..
- Venancio T, Aravind L. CYSTM, a novel cysteine-rich transmembrane module with a role in stress tolerance across eukaryotes. Bioinformatics. 2010;26:149-52 pubmed publisher..Thus, CYSTM proteins are likely to be part of a novel cellular protective mechanism that is widely active in eukaryotes, including humans. Supplementary data are available at Bioinformatics online. ..
- Huang Y, Siwo G, Wuchty S, Ferdig M, Przytycka T. Symmetric Epistasis Estimation (SEE) and its application to dissecting interaction map of Plasmodium falciparum. Mol Biosyst. 2012;8:1544-52 pubmed publisher..falciparum, potentially reflecting host-pathogen interactions or drug-induced selection. ..
- Balasundaram D, Tabor C, Tabor H. Oxygen toxicity in a polyamine-depleted spe2 delta mutant of Saccharomyces cerevisiae. Proc Natl Acad Sci U S A. 1993;90:4693-7 pubmed..However, since the mutant cells still required spermidine or spermine for growth when incubated under strictly anaerobic conditions, polyamines must also have other essential functions. ..
- Hinnebusch A, Asano K, Olsen D, Phan L, Nielsen K, VALASEK L. Study of translational control of eukaryotic gene expression using yeast. Ann N Y Acad Sci. 2004;1038:60-74 pubmed..Thus, apart from its critical role in the starvation response, GCN4 regulation is a valuable tool for dissecting the contributions of multiple translation factors in the eukaryotic initiation pathway. ..
- Calado R, Graf S, Wilkerson K, Kajigaya S, Ancliff P, Dror Y, et al. Mutations in the SBDS gene in acquired aplastic anemia. Blood. 2007;110:1141-6 pubmed..We propose that heterozygosity for the 258 + 2 T>C SBDS mutation predisposes to AA by accelerating telomere shortening of leukocytes via a telomerase-independent mechanism. ..
- Monaghan E, Gable K, Dunn T. Mutations in the Lcb2p subunit of serine palmitoyltransferase eliminate the requirement for the TSC3 gene in Saccharomyces cerevisiae. Yeast. 2002;19:659-70 pubmed
- Yoon S, Govind C, Qiu H, Kim S, Dong J, Hinnebusch A. Recruitment of the ArgR/Mcm1p repressor is stimulated by the activator Gcn4p: a self-checking activation mechanism. Proc Natl Acad Sci U S A. 2004;101:11713-8 pubmed..By recruiting an arginine-regulated repressor, Gcn4p can precisely modulate its activation function at ARG1 according to the availability of arginine. ..
- Qiu H, Hu C, Zhang F, Hwang G, Swanson M, Boonchird C, et al. Interdependent recruitment of SAGA and Srb mediator by transcriptional activator Gcn4p. Mol Cell Biol. 2005;25:3461-74 pubmed..Thus, while Tra1p can bind directly to Gcn4p in vitro, it requires other SAGA subunits for efficient recruitment in vivo. ..
- Boehm A, Higgins J, Franzusoff A, Schlom J, Hodge J. Concurrent vaccination with two distinct vaccine platforms targeting the same antigen generates phenotypically and functionally distinct T-cell populations. Cancer Immunol Immunother. 2010;59:397-408 pubmed publisher..These studies provide the rationale for future clinical studies investigating concurrent administration of vaccine platforms targeting a single antigen to enhance the antigen-specific immune response. ..
- Wickner R, Shewmaker F, Edskes H, Kryndushkin D, Nemecek J, McGlinchey R, et al. Prion amyloid structure explains templating: how proteins can be genes. FEMS Yeast Res. 2010;10:980-91 pubmed publisher..The prion domain sequences generally vary more rapidly in evolution than does the remainder of the molecule, producing a barrier to prion transmission, perhaps selected in evolution by this protection. ..
- Romanienko P, Camerini Otero R. Cloning, characterization, and localization of mouse and human SPO11. Genomics. 1999;61:156-69 pubmed..Human SPO11 was also detected in several somatic tissues. Mouse Spo11 was localized to chromosome 2H4, and human SPO11 was localized to chromosome 20q13.2-q13.3, a region amplified in some breast and ovarian tumors. ..
- Jeong H, Backlund P, CHEN H, Karavanov A, Crouch R. RNase H2 of Saccharomyces cerevisiae is a complex of three proteins. Nucleic Acids Res. 2004;32:407-14 pubmed..The multisubunit nature of S.cerevisiae RNase H2 may be important both for structural purposes and to provide a means of interacting with other proteins involved in DNA replication/repair and transcription. ..
- Liu C, Pouliot J, Nash H. The role of TDP1 from budding yeast in the repair of DNA damage. DNA Repair (Amst). 2004;3:593-601 pubmed..The relationship between the role of TDP1 in Saccharomyces and its role in metazoans is discussed. ..
- Zhou Z, Feng H, Zhou B, Ghirlando R, Hu K, Zwolak A, et al. Structural basis for recognition of centromere histone variant CenH3 by the chaperone Scm3. Nature. 2011;472:234-7 pubmed publisher..Scm3(CBD) induces major conformational changes and sterically occludes DNA-binding sites in the structure of Cse4 and H4. These findings have implications for the assembly and architecture of the centromeric nucleosome. ..
- Pouliot J, Yao K, Robertson C, Nash H. Yeast gene for a Tyr-DNA phosphodiesterase that repairs topoisomerase I complexes. Science. 1999;286:552-5 pubmed..The presence of this gene in humans may have implications for the effectiveness of topoisomerase I poisons, such as the camptothecins, in chemotherapy. ..
- Clark D. Nucleosome positioning, nucleosome spacing and the nucleosome code. J Biomol Struct Dyn. 2010;27:781-93 pubmed..Such a code might facilitate the shunting of nucleosomes from one array to another by ATP-dependent chromatin remodelling machines. ..
- Srinivasan S, Seaman M, Nemoto Y, Daniell L, Suchy S, Emr S, et al. Disruption of three phosphatidylinositol-polyphosphate 5-phosphatase genes from Saccharomyces cerevisiae results in pleiotropic abnormalities of vacuole morphology, cell shape, and osmohomeostasis. Eur J Cell Biol. 1997;74:350-60 pubmed..No defect in carboxypeptidase Y sorting was seen in a processing and targeting assay. Abnormal actin cytoskeleton morphology was present in some of the strains carrying mutations in two of the genes. ..
- Lee J, Choi S, Roll Mecak A, Burley S, Dever T. Universal conservation in translation initiation revealed by human and archaeal homologs of bacterial translation initiation factor IF2. Proc Natl Acad Sci U S A. 1999;96:4342-7 pubmed..We propose a universally conserved function for IF2 in facilitating the proper binding of initiator methionyl-tRNA to the ribosomal P site. ..
- Kim Y, McLaughlin N, Lindstrom K, Tsukiyama T, Clark D. Activation of Saccharomyces cerevisiae HIS3 results in Gcn4p-dependent, SWI/SNF-dependent mobilization of nucleosomes over the entire gene. Mol Cell Biol. 2006;26:8607-22 pubmed..We propose that Gcn4p stimulates nucleosome mobilization over the entire HIS3 gene by the SWI/SNF complex. We suggest that the net effect of interplay among remodeling machines at HIS3 is to create a highly dynamic chromatin structure. ..
- Shi H, Bencze K, Stemmler T, Philpott C. A cytosolic iron chaperone that delivers iron to ferritin. Science. 2008;320:1207-10 pubmed publisher..Depletion of PCBP1 in human cells inhibited ferritin iron loading and increased cytosolic iron pools. Thus, PCBP1 can function as a cytosolic iron chaperone in the delivery of iron to ferritin. ..
- Webster M, McCaffery J, COHEN FIX O. Vesicle trafficking maintains nuclear shape in Saccharomyces cerevisiae during membrane proliferation. J Cell Biol. 2010;191:1079-88 pubmed publisher..Moreover, flares may facilitate maintenance of a constant nuclear/cell volume ratio in the face of altered membrane proliferation. ..
- Ron D, Zannini M, Lewis M, Wickner R, Hunt L, Graziani G, et al. A region of proto-dbl essential for its transforming activity shows sequence similarity to a yeast cell cycle gene, CDC24, and the human breakpoint cluster gene, bcr. New Biol. 1991;3:372-9 pubmed..Thus, our findings indicate that the conserved region is an essential domain that may reflect important functional similarities among these otherwise highly divergent molecules. ..
- Yuan D, Stearman R, Dancis A, Dunn T, Beeler T, Klausner R. The Menkes/Wilson disease gene homologue in yeast provides copper to a ceruloplasmin-like oxidase required for iron uptake. Proc Natl Acad Sci U S A. 1995;92:2632-6 pubmed
- Vazquez de Aldana C, Dever T, Hinnebusch A. Mutations in the alpha subunit of eukaryotic translation initiation factor 2 (eIF-2 alpha) that overcome the inhibitory effect of eIF-2 alpha phosphorylation on translation initiation. Proc Natl Acad Sci U S A. 1993;90:7215-9 pubmed..These mutations may identify a region in eIF-2 alpha that participates directly in a physical interaction with the GCN3 subunit of eIF-2B. ..
- Philpott C, Rashford J, Yamaguchi Iwai Y, Rouault T, Dancis A, Klausner R. Cell-cycle arrest and inhibition of G1 cyclin translation by iron in AFT1-1(up) yeast. EMBO J. 1998;17:5026-36 pubmed..Instead, this reduction occurs at the level of Cln2 translation, a recently recognized locus of cell-cycle control in yeast. ..
- Vogel J, Kristie T. The novel coactivator C1 (HCF) coordinates multiprotein enhancer formation and mediates transcription activation by GABP. EMBO J. 2000;19:683-90 pubmed..The interaction and coordinated assembly of the enhancer proteins by the C1 factor may be critical for the regulation of the HSV lytic-latent cycle. ..
- Wickner R, Shewmaker F, Kryndushkin D, Edskes H. Protein inheritance (prions) based on parallel in-register beta-sheet amyloid structures. Bioessays. 2008;30:955-64 pubmed publisher..This property of self-reproduction, in turn, allows these proteins to act as de facto genes, encoding heritable information. ..
- Kryndushkin D, Shewmaker F, Wickner R. Curing of the [URE3] prion by Btn2p, a Batten disease-related protein. EMBO J. 2008;27:2725-35 pubmed publisher..Btn2p curing requires cell division, and our results suggest that Btn2p is part of a system, reminiscent of the mammalian aggresome, that collects aggregates preventing their efficient distribution to progeny cells. ..
- Maraia R, Lamichhane T. 3' processing of eukaryotic precursor tRNAs. Wiley Interdiscip Rev RNA. 2011;2:362-75 pubmed publisher..A pathway is proposed in which 3' oligo(U) length is a primary determinant of La binding with subsequent steps distinguished by 3'-endo versus exo nucleases,chaperone activities, and nuclear surveillance. ..
- Au W, Dawson A, Rawson D, Taylor S, Baker R, Basrai M. A novel role of the N terminus of budding yeast histone H3 variant Cse4 in ubiquitin-mediated proteolysis. Genetics. 2013;194:513-8 pubmed publisher..Here, we demonstrate a role for ubiquitination of the N terminus of Cse4 in regulating Cse4 proteolysis for faithful chromosome segregation and a role for Doa1 in ubiquitination of Cse4. ..