Genomes and Genes
Experts and Doctors on molecular sequence data in Bethesda, Maryland, United States
Locale: Bethesda, Maryland, United States
Topic: molecular sequence data
Publications555 found, 100 shown here
- Kim M, Lee J, Chung J. In vivo transcription factor recruitment during thyroid hormone receptor-mediated activation. Proc Natl Acad Sci U S A. 1999;96:10092-7 pubmed..On the basis of these findings, we present a model that illustrates the molecular mechanism of TR-mediated activation in vivo. ..
- Ahmad F, Cong L, Stenson Holst L, Wang L, Rahn Landstrom T, Pierce J, et al. Cyclic nucleotide phosphodiesterase 3B is a downstream target of protein kinase B and may be involved in regulation of effects of protein kinase B on thymidine incorporation in FDCP2 cells. J Immunol. 2000;164:4678-88 pubmed..These results suggest that PDE3B, not PDE4, is a target of PKB and that activated PDE3B may regulate cAMP pools that modulate effects of PKB on thymidine incorporation and BAD phosphorylation in FDCP2 cells. ..
- Wang H, Parry D, Jones L, Idler W, Marekov L, Steinert P. In vitro assembly and structure of trichocyte keratin intermediate filaments: a novel role for stabilization by disulfide bonding. J Cell Biol. 2000;151:1459-68 pubmed..Our data suggest a novel role for disulfide bond cross linking in stabilization of these IF and the tissues containing them. ..
- Schmidt A, Wenzke D, McAuliffe J, St Claire M, Elkins W, Murphy B, et al. Mucosal immunization of rhesus monkeys against respiratory syncytial virus subgroups A and B and human parainfluenza virus type 3 by using a live cDNA-derived vaccine based on a host range-attenuated bovine parainfluenza virus type 3 vector backbone. J Virol. 2002;76:1089-99 pubmed
- Horowitz D, Lee E, Mabon S, Misteli T. A cyclophilin functions in pre-mRNA splicing. EMBO J. 2002;21:470-80 pubmed..These results provide an example of the function of a cyclophilin in a complex process and provide insight into the mechanisms of action of cyclophilins. ..
- Carlson B, Hatfield D. Transfer RNAs that insert selenocysteine. Methods Enzymol. 2002;347:24-39 pubmed
- Jerse A, Crow E, Bordner A, Rahman I, Cornelissen C, Moench T, et al. Growth of Neisseria gonorrhoeae in the female mouse genital tract does not require the gonococcal transferrin or hemoglobin receptors and may be enhanced by commensal lactobacilli. Infect Immun. 2002;70:2549-58 pubmed..In summary, these studies support the potential role of nontransferrin, nonhemoglobin iron sources during gonococcal infection of the female genital tract. ..
- Patel V, Aldridge K, Ensley J, Odell E, Boyd A, Jones J, et al. Laminin-gamma2 overexpression in head-and-neck squamous cell carcinoma. Int J Cancer. 2002;99:583-8 pubmed..The data indicate that laminin-gamma2 is frequently overexpressed in HNSCCs and derivative cell lines and that its overexpression is likely to be useful as a marker of head-and-neck squamous malignancy. ..
- Huang Y, Xu L, Sun Y, Nabel G. The assembly of Ebola virus nucleocapsid requires virion-associated proteins 35 and 24 and posttranslational modification of nucleoprotein. Mol Cell. 2002;10:307-16 pubmed..This distinct mechanism of regulation for filovirus assembly suggests new approaches for viral therapies and vaccines for Ebola and related viruses. ..
- Liu Z, Liu Q, Pesce J, Whitmire J, Ekkens M, Foster A, et al. Nippostrongylus brasiliensis can induce B7-independent antigen-specific development of IL-4-producing T cells from naive CD4 T cells in vivo. J Immunol. 2002;169:6959-68 pubmed..10 Th2 cell differentiation; however, DO11.10 T cell cycle progression and migration to the B cell zone are inhibited...
- Xu D, Thompson J, Cisar J. Genetic loci for coaggregation receptor polysaccharide biosynthesis in Streptococcus gordonii 38. J Bacteriol. 2003;185:5419-30 pubmed..Thus, provision of both UDP-Gal and UDP-GalNAc for RPS production by S. gordonii 38 depends on the dual specificity of the epimerase encoded by galE2. ..
- JÃ¤schke H, Neumann S, Moore S, Thomas C, Colson A, Costanzi S, et al. A low molecular weight agonist signals by binding to the transmembrane domain of thyroid-stimulating hormone receptor (TSHR) and luteinizing hormone/chorionic gonadotropin receptor (LHCGR). J Biol Chem. 2006;281:9841-4 pubmed..These findings provide the first evidence that, in contrast to the ectodomain binding of cognate ligands, a LMW agonist can bind to and activate glycoprotein hormone receptors via interaction with their transmembrane domain. ..
- Gonzalez N, Nakagawa T, Mantey S, Sancho V, Uehara H, Katsuno T, et al. Molecular basis for the selectivity of the mammalian bombesin peptide, neuromedin B, for its receptor. J Pharmacol Exp Ther. 2009;331:265-76 pubmed publisher..The effect at position 199 is primarily due to differences in hydrophobicity of the substitution, whereas steric factors and charge of the substitution at position 203 were important determinants of NMB selectivity. ..
- Hirsch F, Germana S, Gustafsson K, Pratt K, Sachs D, LeGuern C. Structure and expression of class II alpha genes in miniature swine. J Immunol. 1992;149:841-6 pubmed
- Pelton J, Torchia D, Meadow N, Wong C, Roseman S. Secondary structure of the phosphocarrier protein IIIGlc, a signal-transducing protein from Escherichia coli, determined by heteronuclear three-dimensional NMR spectroscopy. Proc Natl Acad Sci U S A. 1991;88:3479-83 pubmed
- Klion A, Raghavan N, Brindley P, Nutman T. Cloning and characterization of a species-specific repetitive DNA sequence from Loa loa. Mol Biochem Parasitol. 1991;45:297-305 pubmed..Species specificity of LL3M9REP was confirmed by amplification of the repetitive region using genomic DNA as a template in the polymerase chain reaction...
- Koonin E, Rudd K. SpoU protein of Escherichia coli belongs to a new family of putative rRNA methylases. Nucleic Acids Res. 1993;21:5519 pubmed
- Janz S, Muller J, Shaughnessy J, Potter M. Detection of recombinations between c-myc and immunoglobulin switch alpha in murine plasma cell tumors and preneoplastic lesions by polymerase chain reaction. Proc Natl Acad Sci U S A. 1993;90:7361-5 pubmed..We conclude that t(12;15) is the probable primary, if not initiating, oncogenic step in plasmacytomagenesis. ..
- Bzdega T, Chin H, Kim H, Jung H, Kozak C, Klee W. Regional expression and chromosomal localization of the delta opiate receptor gene. Proc Natl Acad Sci U S A. 1993;90:9305-9 pubmed..The gene, which is present as a single copy, has been mapped to the distal region of mouse Chromosome 4. ..
- Steele F, Chader G, Johnson L, Tombran Tink J. Pigment epithelium-derived factor: neurotrophic activity and identification as a member of the serine protease inhibitor gene family. Proc Natl Acad Sci U S A. 1993;90:1526-30 pubmed..Thus, PEDF could exert a paracrine effect in the embryonic retina, influencing neuronal differentiation by a mechanism that does not involve classic inhibition of serine protease activity. ..
- Hong J, Wilson G, Fox C, Kehrl J. Isolation and characterization of a novel B cell activation gene. J Immunol. 1993;150:3895-904 pubmed..Further characterization of the BL34 gene and its protein should lead to insights to its role in B cell function and the consequences of its over-expression in acute lymphocytic leukemia. ..
- Iacangelo A, Eiden L. Chromogranin A: current status as a precursor for bioactive peptides and a granulogenic/sorting factor in the regulated secretory pathway. Regul Pept. 1995;58:65-88 pubmed
- Hamasaki Katagiri N, Katagiri Y, Tabor C, Tabor H. Spermine is not essential for growth of Saccharomyces cerevisiae: identification of the SPE4 gene (spermine synthase) and characterization of a spe4 deletion mutant. Gene. 1998;210:195-201 pubmed..Tabor, C. W., Tabor, H., 1997. Spermidine biosynthesis in Saccharomyces cerevisiae: Polyamine requirement of a null mutant of the SPE3 gene (spermidine synthase). Gene 187, 35-43]. ..
- Garrett D, Seok Y, Peterkofsky A, Gronenborn A, Clore G. Solution structure of the 40,000 Mr phosphoryl transfer complex between the N-terminal domain of enzyme I and HPr. Nat Struct Biol. 1999;6:166-73 pubmed publisher..The transition state can be formed with minimal changes in overall conformation, and is stabilized in favor of phosphorylated HPr, thereby accounting for the directionality of phosphoryl transfer...
- Huang Y, Maraia R. Comparison of the RNA polymerase III transcription machinery in Schizosaccharomyces pombe, Saccharomyces cerevisiae and human. Nucleic Acids Res. 2001;29:2675-90 pubmed..The idea that the divergence in downstream TFIIIC subunits is associated with differences in pol III termination-related mechanisms that have been noted in the yeast and human systems but not reviewed previously is also considered. ..
- Senkevich T, Moss B. Vaccinia virus H2 protein is an essential component of a complex involved in virus entry and cell-cell fusion. J Virol. 2005;79:4744-54 pubmed..Therefore, H2 and A28 are the only proteins presently known to be specifically required for vaccinia virus entry and are likely components of a fusion complex...
- Goh S, Lee Y, Bhanu N, Cam M, Desper R, Martin B, et al. A newly discovered human alpha-globin gene. Blood. 2005;106:1466-72 pubmed..These results suggest the human genome encodes a previously unrecognized globin member of the avian alpha-D family that is transcribed in a highly regulated pattern in erythroid cells. ..
- Hong S, Haldin C, Lawson N, Weinstein B, Dawid I, Hukriede N. The zebrafish kohtalo/trap230 gene is required for the development of the brain, neural crest, and pronephric kidney. Proc Natl Acad Sci U S A. 2005;102:18473-8 pubmed..These results suggest that critical targets of TRAP230 function may include proteins important for cell mobility, cell sorting, and tissue assembly. ..
- Kametaka S, Moriyama K, Burgos P, Eisenberg E, Greene L, Mattera R, et al. Canonical interaction of cyclin G associated kinase with adaptor protein 1 regulates lysosomal enzyme sorting. Mol Biol Cell. 2007;18:2991-3001 pubmed..Thus, interactions between the PsiG[PDE][PsiLM]-motif sequences in GAK and the AP1-gamma-ear domain are critical for the recruitment of GAK to the TGN and the function of GAK in lysosomal enzyme sorting. ..
- Marini J, Cabral W, Barnes A, Chang W. Components of the collagen prolyl 3-hydroxylation complex are crucial for normal bone development. Cell Cycle. 2007;6:1675-81 pubmed..On a clinical level, defects in the components of the prolyl 3-hydroxylation complex have been shown to be the long-sought cause of severe and lethal recessive osteogenesis imperfecta. ..
- Zurawski D, Mumy K, Faherty C, McCormick B, Maurelli A. Shigella flexneri type III secretion system effectors OspB and OspF target the nucleus to downregulate the host inflammatory response via interactions with retinoblastoma protein. Mol Microbiol. 2009;71:350-68 pubmed publisher..The requirement of multiple T3SS effectors to modulate the innate immune response correlates to the complexity of the human immune system. ..
- Tikhonova A, Van Laethem F, Hanada K, Lu J, Pobezinsky L, Hong C, et al. Î±Î² T cell receptors that do not undergo major histocompatibility complex-specific thymic selection possess antibody-like recognition specificities. Immunity. 2012;36:79-91 pubmed publisher..This study demonstrates that, without MHC-specific thymic selection, Î±Î²TCRs can resemble antibodies in recognizing conformational epitopes on MHC-independent ligands. ..
- Wei H, Xiang L, Wayne A, Chertov O, FitzGerald D, Bera T, et al. Immunotoxin resistance via reversible methylation of the DPH4 promoter is a unique survival strategy. Proc Natl Acad Sci U S A. 2012;109:6898-903 pubmed publisher..Incubation of sensitive cells with the methylation inhibitor 5-azacytidine prevented the emergence of resistant cells, suggesting that this agent in combination with HA22 may be useful in the treatment of some cases of ALL. ..
- Zhang K, Noda M, Vass W, Papageorge A, Lowy D. Identification of small clusters of divergent amino acids that mediate the opposing effects of ras and Krev-1. Science. 1990;249:162-5 pubmed..Because this region in Ras proteins has been implicated in effector function, the results suggest that Krev-1 suppresses ras-induced transformation by interfering with interaction of Ras with its effector...
- Stolow D, Haynes S. Cabeza, a Drosophila gene encoding a novel RNA binding protein, shares homology with EWS and TLS, two genes involved in human sarcoma formation. Nucleic Acids Res. 1995;23:835-43 pubmed..Therefore, it appears that these genes constitute a new sub-family of RNA binding proteins. ..
- Krah R, Kozyavkin S, Slesarev A, Gellert M. A two-subunit type I DNA topoisomerase (reverse gyrase) from an extreme hyperthermophile. Proc Natl Acad Sci U S A. 1996;93:106-10 pubmed..The appearance of this unique structure in a highly conserved enzyme family supports the hypothesis that the methanogens branched from other prokaryotes and eukaryotes very early in evolution...
- Allman D, Jain A, Dent A, Maile R, Selvaggi T, Kehry M, et al. BCL-6 expression during B-cell activation. Blood. 1996;87:5257-68 pubmed..These observations suggest that the germinal center reaction provides unique activation signals to B cells that allow for continued, high-level BCL-6 expression. ..
- Alexander Miller M, Parker K, Tsukui T, Pendleton C, Coligan J, Berzofsky J. Molecular analysis of presentation by HLA-A2.1 of a promiscuously binding V3 loop peptide from the HIV-envelope protein to human cytotoxic T lymphocytes. Int Immunol. 1996;8:641-9 pubmed..Agretopic residue similarities with the hepatitis B nucleocapsid decamer suggest that these residues may comprise an alternative motif of anchors utilized by decamers for binding to HLA-A2.1. ..
- van Rhee A, Fischer B, van Galen P, Jacobson K. Modelling the P2Y purinoceptor using rhodopsin as template. Drug Des Discov. 1995;13:133-54 pubmed
- Jaworski C, Wistow G. LP2, a differentiation-associated lipid-binding protein expressed in bovine lens. Biochem J. 1996;320 ( Pt 1):49-54 pubmed..LP2 is thus another potential target for oxidative stress during cataract formation in lens. ..
- Rhee S, Martin R, Rosner J, Davies D. A novel DNA-binding motif in MarA: the first structure for an AraC family transcriptional activator. Proc Natl Acad Sci U S A. 1998;95:10413-8 pubmed..Extensive interactions between the recognition helices and the DNA major groove provide the sequence specificity...
- Asano K, Krishnamoorthy T, Phan L, Pavitt G, Hinnebusch A. Conserved bipartite motifs in yeast eIF5 and eIF2Bepsilon, GTPase-activating and GDP-GTP exchange factors in translation initiation, mediate binding to their common substrate eIF2. EMBO J. 1999;18:1673-88 pubmed..Thus, the bipartite motif in eIF5 appears to be multifunctional, stimulating its recruitment to the 40S pre-initiation complex through interaction with eIF3 in addition to binding of its substrate eIF2. ..
- Martinez A, Kapas S, Miller M, Ward Y, Cuttitta F. Coexpression of receptors for adrenomedullin, calcitonin gene-related peptide, and amylin in pancreatic beta-cells. Endocrinology. 2000;141:406-11 pubmed..These data further implicate AM and the other components of its peptide family as important regulators of insulin release. ..
- Wassarman D, Aoyagi N, Pile L, Schlag E. TAF250 is required for multiple developmental events in Drosophila. Proc Natl Acad Sci U S A. 2000;97:1154-9 pubmed..Because this region is not conserved in the yeast TAF250 homologue, TAF145, it may define an activity for TAF250 that is unique to higher eukaryotes. ..
- Niimi T, Munakata M, Keck Waggoner C, Popescu N, Levitt R, Hisada M, et al. A polymorphism in the human UGRP1 gene promoter that regulates transcription is associated with an increased risk of asthma. Am J Hum Genet. 2002;70:718-25 pubmed..1 times more likely to have asthma than were those with the wild-type allele (G/G). ..
- Dalakas M, Dagvadorj A, Goudeau B, Park K, Takeda K, Simon Casteras M, et al. Progressive skeletal myopathy, a phenotypic variant of desmin myopathy associated with desmin mutations. Neuromuscul Disord. 2003;13:252-8 pubmed..Progressive skeletal myopathy is a rare phenotypic variant of desmin myopathy allelic to the more frequent cardio-skeletal form. ..
- Agarwal R, Caspi R. Rodent models of experimental autoimmune uveitis. Methods Mol Med. 2004;102:395-419 pubmed..The ability to induce EAU in various gene-manipulated, including HLA-transgenic, mouse strains makes the EAU model suitable for the study of basic mechanisms as well as in clinically relevant interventions. ..
- Dyer K, Rosenberg H, Zhang J. Isolation, characterization, and evolutionary divergence of mouse RNase 6: evidence for unusual evolution in rodents. J Mol Evol. 2004;59:657-65 pubmed..66. These data suggest that the ancestral rodent RNase 6 was subject to accelerated evolution, resulting in the conserved modern gene, which most likely plays an important role in mouse physiology. ..
- Wang L, Chmelik R, Tang D, Nirenberg M. Identification and analysis of vnd/NK-2 homeodomain binding sites in genomic DNA. Proc Natl Acad Sci U S A. 2005;102:7097-102 pubmed..The DNase I footprinting result showed that there are two strong binding sites and five weak binding sites in the fragment between -385 and -675 bp from the transcription start site of the vnd/NK-2 gene. ..
- Bibb L, King N, Kunkle C, Schmitt M. Analysis of a heme-dependent signal transduction system in Corynebacterium diphtheriae: deletion of the chrAS genes results in heme sensitivity and diminished heme-dependent activation of the hmuO promoter. Infect Immun. 2005;73:7406-12 pubmed..Transcriptional analysis of the chrAS operon revealed that it was not autoregulated or affected by iron or heme levels...
- Koonin E, Makarova K, Elkins J. Orthologs of the small RPB8 subunit of the eukaryotic RNA polymerases are conserved in hyperthermophilic Crenarchaeota and "Korarchaeota". Biol Direct. 2007;2:38 pubmed..These findings suggest that all 12 core subunits of eukaryotic RNAPs were already present in the last common ancestor of the extant archaea...
- Iyer L, Abhiman S, Aravind L. A new family of polymerases related to superfamily A DNA polymerases and T7-like DNA-dependent RNA polymerases. Biol Direct. 2008;3:39 pubmed publisher..They also provide evidence that enigmatic DNA viruses, such as Sputnik, might have emerged from mobile elements coding these polymerases. ..
- Dev K, Santangelo T, Rothenburg S, Neculai D, Dey M, Sicheri F, et al. Archaeal aIF2B interacts with eukaryotic translation initiation factors eIF2alpha and eIF2Balpha: Implications for aIF2B function and eIF2B regulation. J Mol Biol. 2009;392:701-22 pubmed publisher..In this model, the evolutionarily conserved regions and sites of regulatory mutations in the three eIF2B subunits in yeast are juxtaposed in one continuous binding surface for phosphorylated eIF2alpha. ..
- Raman J, Guan Y, Perrine C, Gerken T, Tabak L. UDP-N-acetyl-?-D-galactosamine:polypeptide N-acetylgalactosaminyltransferases: completion of the family tree. Glycobiology. 2012;22:768-77 pubmed publisher..Our findings provide a comprehensive view of the complexities of mucin-type O-glycan formation and provide insight into the underlying mechanisms employed to heavily decorate mucins and mucin-like domains with carbohydrate. ..
- Sugui J, Peterson S, Clark L, Nardone G, Folio L, Riedlinger G, et al. Aspergillus tanneri sp. nov., a new pathogen that causes invasive disease refractory to antifungal therapy. J Clin Microbiol. 2012;50:3309-17 pubmed publisher..Our phenotypic and genetic characterization of the new species and the case reports will assist future diagnosis of infection caused by A. tanneri and lead to more appropriate patient management. ..
- States D, Walseth T, Lee H. Similarities in amino acid sequences of Aplysia ADP-ribosyl cyclase and human lymphocyte antigen CD38. Trends Biochem Sci. 1992;17:495 pubmed
- Datiles M, Schumer D, Zigler J, Russell P, Anderson L, Garland D. Two-dimensional gel electrophoretic analysis of human lens proteins. Curr Eye Res. 1992;11:669-77 pubmed..Two-dimensional gel electrophoresis provides a sensitive and practical technique for characterizing all of the proteins of the human lens. ..
- Tsarev S, Emerson S, Reyes G, Tsareva T, Legters L, Malik I, et al. Characterization of a prototype strain of hepatitis E virus. Proc Natl Acad Sci U S A. 1992;89:559-63 pubmed..Identification of this region as hypervariable was obtained by partial sequencing of a third isolate of hepatitis E virus from Kirgizia...
- Hohjoh H, Singer M. Cytoplasmic ribonucleoprotein complexes containing human LINE-1 protein and RNA. EMBO J. 1996;15:630-9 pubmed
- Rosenberg H, Dyer K. Diversity among the primate eosinophil-derived neurotoxin genes: a specific C-terminal sequence is necessary for enhanced ribonuclease activity. Nucleic Acids Res. 1997;25:3532-6 pubmed
- Kays W, Piatigorsky J. Aldehyde dehydrogenase class 3 expression: identification of a cornea-preferred gene promoter in transgenic mice. Proc Natl Acad Sci U S A. 1997;94:13594-9 pubmed..We demonstrate regulatory sequences capable of directing cornea-specific gene expression, affording the opportunity for genetic engineering in this transparent tissue. ..
- Donadel G, Marinos N, DeSilva M, Lu J, Notkins A, Lan M. Molecular cloning and characterization of a highly basic protein, IA-4, expressed in pancreatic islets and brain. Neuroendocrinology. 1998;67:190-6 pubmed..The high expression of IA-4 protein in neuroendocrine cells and its unique amino acid sequence suggest that IA-4 may have an important, but still undetermined, function in these special cell types. ..
- Xiang B, Weiler S, Nirenberg M, Ferretti J. Structural basis of an embryonically lethal single Ala --> Thr mutation in the vnd/NK-2 homeodomain. Proc Natl Acad Sci U S A. 1998;95:7412-6 pubmed..In the A35T mutant this amide proton resonance is shifted upfield by 1.27 ppm relative to the resonance frequency for the wild-type analog, thereby indicating a significant alteration of this i,i-5 hydrogen bond. ..
- Huang K, Louis J, Donaldson L, Lim F, Sharrocks A, Clore G. Solution structure of the MEF2A-DNA complex: structural basis for the modulation of DNA bending and specificity by MADS-box transcription factors. EMBO J. 2000;19:2615-28 pubmed..The structure of the MEF2S domain is entirely different from that of the equivalent SAM domain in SRF and MCM1, accounting for the absence of cross-reactivity with other proteins that interact with these transcription factors. ..
- Du J, Feng L, Yang F, Lu B. Activity- and Ca(2+)-dependent modulation of surface expression of brain-derived neurotrophic factor receptors in hippocampal neurons. J Cell Biol. 2000;150:1423-34 pubmed..These findings may help us to understand the role of neuronal activity in neurotrophin function and the mechanism for receptor tyrosine kinase signaling. ..
- Remaley A, Bark S, Walts A, Freeman L, Shulenin S, Annilo T, et al. Comparative genome analysis of potential regulatory elements in the ABCG5-ABCG8 gene cluster. Biochem Biophys Res Commun. 2002;295:276-82 pubmed..In summary, several potential regulatory elements were found for the ABCG5 and ABCG8 genes, and the intergenic region was found to act as a bidirectional promoter. ..
- Monaghan E, Gable K, Dunn T. Mutations in the Lcb2p subunit of serine palmitoyltransferase eliminate the requirement for the TSC3 gene in Saccharomyces cerevisiae. Yeast. 2002;19:659-70 pubmed
- Difilippantonio M, Petersen S, Chen H, Johnson R, Jasin M, Kanaar R, et al. Evidence for replicative repair of DNA double-strand breaks leading to oncogenic translocation and gene amplification. J Exp Med. 2002;196:469-80 pubmed..Thus, mice deficient in NHEJ provide excellent models to study the etiology of unbalanced translocations and amplification events during tumorigenesis. ..
- Sloan S, Rutkai E, King R, Velikodvorskaya T, Weisberg R. Protection of antiterminator RNA by the transcript elongation complex. Mol Microbiol. 2007;63:1197-208 pubmed..These results argue that the continuity of the RNA that connects put sequences to the growing point is not required for persistence of the antiterminating modification in vivo...
- Maeda K, Das D, Yin P, Tsuchiya K, Ogata Aoki H, Nakata H, et al. Involvement of the second extracellular loop and transmembrane residues of CCR5 in inhibitor binding and HIV-1 fusion: insights into the mechanism of allosteric inhibition. J Mol Biol. 2008;381:956-74 pubmed publisher..The data should not only help delineate the dynamics of CCR5 following inhibitor binding but also aid in designing CCR5 inhibitors that are more potent against HIV-1 and prevent or delay the emergence of resistant HIV-1 variants. ..
- Daniel J, Pellegrini M, Lee J, Paull T, Feigenbaum L, Nussenzweig A. Multiple autophosphorylation sites are dispensable for murine ATM activation in vivo. J Cell Biol. 2008;183:777-83 pubmed publisher..These results lend further support to the notion that ATM autophosphorylation correlates with the DNA damage-induced activation of the kinase but is not required for ATM function in vivo. ..
- Jenkins L, Yamaguchi H, Hayashi R, Cherry S, Tropea J, Miller M, et al. Two distinct motifs within the p53 transactivation domain bind to the Taz2 domain of p300 and are differentially affected by phosphorylation. Biochemistry. 2009;48:1244-55 pubmed publisher..However, the more negative value of DeltaC(p) for Taz2 binding to the first (-330 cal/(mol.K)) compared to the second site (-234 cal/(mol.K)) suggests that the importance of nonpolar and polar interactions differs between the two sites. ..
- De Vega S, Iwamoto T, Yamada Y. Fibulins: multiple roles in matrix structures and tissue functions. Cell Mol Life Sci. 2009;66:1890-902 pubmed publisher..In this review, we describe the structure and function of these proteins and discuss the implication of fibulins in development and diseases. ..
- Kash J, Qi L, Dugan V, Jagger B, Hrabal R, Memoli M, et al. Prior infection with classical swine H1N1 influenza viruses is associated with protective immunity to the 2009 pandemic H1N1 virus. Influenza Other Respir Viruses. 2010;4:121-7 pubmed publisher..Implications of these findings are discussed in the context of accumulating data suggesting partial protection of older persons during the 2009 pandemic. ..
- Sastalla I, Maltese L, Pomerantseva O, Pomerantsev A, Keane Myers A, Leppla S. Activation of the latent PlcR regulon in Bacillus anthracis. Microbiology. 2010;156:2982-93 pubmed publisher..In contrast, the toxicity of bacterial culture supernatants from a PlcR-positive strain towards murine macrophages occurred independently of anthrolysin O expression in vitro and in vivo...
- Zhou Z, Feng H, Zhou B, Ghirlando R, Hu K, Zwolak A, et al. Structural basis for recognition of centromere histone variant CenH3 by the chaperone Scm3. Nature. 2011;472:234-7 pubmed publisher..Scm3(CBD) induces major conformational changes and sterically occludes DNA-binding sites in the structure of Cse4 and H4. These findings have implications for the assembly and architecture of the centromeric nucleosome. ..
- Liu M, Eiden M. Role of human endogenous retroviral long terminal repeats (LTRs) in maintaining the integrity of the human germ line. Viruses. 2011;3:901-5 pubmed publisher..The ability of ERV-9 regulatory regions to contribute to the maintenance of male germ line stability is yet another example of how ERVs have evolved to serve an important function in the physiology of their human hosts. ..
- Lipes M, Napolitano M, Jeang K, Chang N, Leonard W. Identification, cloning, and characterization of an immune activation gene. Proc Natl Acad Sci U S A. 1988;85:9704-8 pubmed..Using a baculovirus expression system, we have shown that this gene encodes a secreted product. It is therefore possible that Act-2 represents a newly discovered cytokine. ..
- Si J, Borst D, Redmond T, Nickerson J. Cloning of cDNAs encoding human interphotoreceptor retinoid-binding protein (IRBP) and comparison with bovine IRBP sequences. Gene. 1989;80:99-108 pubmed..Thus, the human sequence is virtually full length, is similar to the bovine sequence, and contains a striking fourfold repeat. ..
- Abbotts J, Sengupta D, Zon G, Wilson S. Studies on the mechanism of Escherichia coli DNA polymerase I large fragment. Effect of template sequence and substrate variation on termination of synthesis. J Biol Chem. 1988;263:15094-103 pubmed..The results are consistent with a model in which termination occurs with several enzyme forms that are in equilibrium in an ordered catalytic mechanism. ..
- Singer D, Hare J, Golding H, Flaherty L, Rudikoff S. Characterization of a new subfamily of class I genes in the H-2 complex of the mouse. Immunogenetics. 1988;28:13-21 pubmed..Examination of various species of wild mice demonstrates the presence of a discrete Mb1 subfamily over long evolutionary periods of time. ..
- Nicholls P, Johnson V, Blanford M, Andrew S. An improved method for generating single-chain antibodies from hybridomas. J Immunol Methods. 1993;165:81-91 pubmed..Our method will be generally useful for screening single-chain antibodies for function prior to large scale production in vivo. ..
- Wolffe A, Khochbin S, Dimitrov S. What do linker histones do in chromatin?. Bioessays. 1997;19:249-55 pubmed..Surprisingly, H1 can both activate and repress transcription. We discuss how this architectural protein might accomplish this important regulatory role. ..
- Sakata H, Stahl S, Taylor W, Rosenberg J, Sakaguchi K, Wingfield P, et al. Heparin binding and oligomerization of hepatocyte growth factor/scatter factor isoforms. Heparan sulfate glycosaminoglycan requirement for Met binding and signaling. J Biol Chem. 1997;272:9457-63 pubmed..These findings establish the importance of the N domain for heparin-ligand and ligand-ligand interactions, and demonstrate a crucial role for HSGAG in receptor binding and signal transduction. ..
- Qian X, Esteban L, Vass W, Upadhyaya C, Papageorge A, Yienger K, et al. The Sos1 and Sos2 Ras-specific exchange factors: differences in placental expression and signaling properties. EMBO J. 2000;19:642-54 pubmed..We conclude that Sos1 participates in both short- and long-term signaling, while Sos2-dependent signals are predominantly short-term. ..
- Kirschner L, Sandrini F, Monbo J, Lin J, Carney J, Stratakis C. Genetic heterogeneity and spectrum of mutations of the PRKAR1A gene in patients with the carney complex. Hum Mol Genet. 2000;9:3037-46 pubmed..CNC is the first human disease recognized to be caused by mutations of the PKA holoenzyme, a critical component of cellular signaling. ..
- Nagashima M, Shiseki M, Miura K, Hagiwara K, Linke S, Pedeux R, et al. DNA damage-inducible gene p33ING2 negatively regulates cell proliferation through acetylation of p53. Proc Natl Acad Sci U S A. 2001;98:9671-6 pubmed..Furthermore, p33ING2 expression increases the acetylation of p53 at Lys-382. Taken together, p33ING2 is a DNA damage-inducible gene that negatively regulates cell proliferation through activation of p53 by enhancing its acetylation. ..
- Worley B, Van den Broeke L, Goletz T, Pendleton C, Daschbach E, Thomas E, et al. Antigenicity of fusion proteins from sarcoma-associated chromosomal translocations. Cancer Res. 2001;61:6868-75 pubmed..These may be useful for the development of novel immunotherapies for sarcoma patients with appropriate HLA molecules and tumors bearing these translocations. ..