Experts and Doctors on fungal rna in Bethesda, Maryland, United States
Locale: Bethesda, Maryland, United States
Topic: fungal rna
Veres Z, Stadtman T. A purified selenophosphate-dependent enzyme from Salmonella typhimurium catalyzes the replacement of sulfur in 2-thiouridine residues in tRNAs with selenium. Proc Natl Acad Sci U S A. 1994;91:8092-6 pubmed
..A labeled compound in the nucleoside mixture was coeluted with authentic 5-methylaminomethyl-2-selenouridine...
VALASEK L, Phan L, Schoenfeld L, Valášková V, Hinnebusch A. Related eIF3 subunits TIF32 and HCR1 interact with an RNA recognition motif in PRT1 required for eIF3 integrity and ribosome binding. EMBO J. 2001;20:891-904 pubmed
..Hence, the PRT1 RRM is crucial for the integrity and ribosome-binding activity of eIF3. ..
Bacikova D, Horowitz D. Genetic and functional interaction of evolutionarily conserved regions of the Prp18 protein and the U5 snRNA. Mol Cell Biol. 2005;25:2107-16 pubmed
Jivotovskaya A, VALASEK L, Hinnebusch A, Nielsen K. Eukaryotic translation initiation factor 3 (eIF3) and eIF2 can promote mRNA binding to 40S subunits independently of eIF4G in yeast. Mol Cell Biol. 2006;26:1355-72 pubmed
Karpova T, Kim M, Spriet C, Nalley K, Stasevich T, Kherrouche Z, et al
. Concurrent fast and slow cycling of a transcriptional activator at an endogenous promoter. Science. 2008;319:466-9 pubmed publisher
..These findings counter the prevailing view that slow cycling initiates transcription. ..
Dong J, Nanda J, Rahman H, Pruitt M, Shin B, Wong C, et al
. Genetic identification of yeast 18S rRNA residues required for efficient recruitment of initiator tRNA(Met) and AUG selection. Genes Dev. 2008;22:2242-55 pubmed publisher
..Hence, the nonconserved 928:1389 base pair in h28, plus conserved 18S rRNA residues corresponding to P-site contacts in bacterial ribosomes, are critical for efficient Met-tRNA(i)(Met) binding and AUG selection in eukaryotes. ..
Dev K, Qiu H, Dong J, Zhang F, Barthlme D, Hinnebusch A. The beta/Gcd7 subunit of eukaryotic translation initiation factor 2B (eIF2B), a guanine nucleotide exchange factor, is crucial for binding eIF2 in vivo. Mol Cell Biol. 2010;30:5218-33 pubmed publisher
..In aggregate, these findings provide compelling evidence that ?/Gcd7 is crucial for binding of substrate by eIF2B in vivo, beyond its dispensable regulatory role in the inhibition of eIF2B by eIF (?P). ..