Genomes and Genes
Experts and Doctors on cultured cells in College Park, Maryland, United States
Locale: College Park, Maryland, United States
Topic: cultured cells
- Heuck K, Ellestad L, Proudman J, Porter T. Somatotropin response in vitro to corticosterone and triiodothyronine during chick embryonic development: Involvement of type I and type II glucocorticoid receptors. Domest Anim Endocrinol. 2009;36:186-96 pubmed publisher..We conclude that positive somatotroph responses to CORT are lost during chicken embryonic development and that GR is the primary regulator of CORT-induced GH secretion. ..
- Wamer W, Vath P, Falvey D. In vitro studies on the photobiological properties of aloe emodin and aloin A. Free Radic Biol Med. 2003;34:233-42 pubmed..Although aloin A was not directly photocytotoxic, we found that human skin fibroblasts can metabolize aloin A to aloe emodin. ..
- Song H, Santi N, Evensen O, Vakharia V. Molecular determinants of infectious pancreatic necrosis virus virulence and cell culture adaptation. J Virol. 2005;79:10289-99 pubmed..Highly virulent isolates possess residues Thr217 and Ala221; moderate- to low-virulence strains have Pro217 and Ala221; and strains containing Thr221 are almost avirulent, irrespective of the residue at position 217. ..
- Yang X, Izadi H, Coleman A, Wang P, Ma Y, Fikrig E, et al. Borrelia burgdorferi lipoprotein BmpA activates pro-inflammatory responses in human synovial cells through a protein moiety. Microbes Infect. 2008;10:1300-8 pubmed publisher..These results show that a protein moiety of BmpA can induce cytokine responses in synovial cells via activation of the NF-kappaB and p38 MAP kinase pathways and thus, could potentially contribute to the genesis of Lyme arthritis...
- Yang Z, Mosser D, Zhang X. Activation of the MAPK, ERK, following Leishmania amazonensis infection of macrophages. J Immunol. 2007;178:1077-85 pubmed..Thus, our findings reveal an important role of MAPK, ERK signaling in the pathogenesis of Leishmania infection. ..
- Shah S, Nolan R, Davis E, Stokin G, Niesman I, Canto I, et al. Examination of potential mechanisms of amyloid-induced defects in neuronal transport. Neurobiol Dis. 2009;36:11-25 pubmed publisher..ROS and membrane permeability are also unlikely to be responsible for fibril-mediated toxicity. Collectively, our results indicate that multiple initiating factors converge upon pathways of defective transport. ..
- Sohn E, Paape M, Connor E, Bannerman D, Fetterer R, Peters R. Bacterial lipopolysaccharide stimulates bovine neutrophil production of TNF-alpha, IL-1beta, IL-12 and IFN-gamma. Vet Res. 2007;38:809-18 pubmed
- Richard K, Pierce S, Song W. The agonists of TLR4 and 9 are sufficient to activate memory B cells to differentiate into plasma cells in vitro but not in vivo. J Immunol. 2008;181:1746-52 pubmed..Thus, although memory B cells are sensitive to TLR agonists in vitro, TLR agonists alone appear to have little affect on B cell memory in vivo. ..
- Wong S, Shih R, Schoene N, Lei K. Zinc-induced G2/M blockage is p53 and p21 dependent in normal human bronchial epithelial cells. Am J Physiol Cell Physiol. 2008;294:C1342-9 pubmed publisher..Thus high zinc status in NHBE cells upregulates p53 expression which in turn elevates p21 that eventually induces G2/M blockage. ..
- Srinivasan M, Frauwirth K. Reciprocal NFAT1 and NFAT2 nuclear localization in CD8+ anergic T cells is regulated by suboptimal calcium signaling. J Immunol. 2007;179:3734-41 pubmed..Thus, our work provides new insight into how T cell stimulation conditions might dictate specific NFAT isoform activation and implicates NFAT2 involvement in the expression of anergy-related genes. ..
- Patrone J, Bish S, Stein D. TNF-alpha-independent IL-8 expression: alterations in bacterial challenge dose cause differential human monocytic cytokine response. J Immunol. 2006;177:1314-22 pubmed..Therefore, we propose a mechanism where a low MOI gonococcal challenge results in diminished AP-1 activity and TNF-alpha production while IL-8 levels remain constant. ..
- Bohsali A, Abdalla H, Velmurugan K, Briken V. The non-pathogenic mycobacteria M. smegmatis and M. fortuitum induce rapid host cell apoptosis via a caspase-3 and TNF dependent pathway. BMC Microbiol. 2010;10:237 pubmed publisher..These observations lend support to the hypothesis that the strong induction of the innate immune response is a major reason for the lack of pathogenicity in fast-growing mycobacteria. ..
- Flannery A, Czibener C, Andrews N. Palmitoylation-dependent association with CD63 targets the Ca2+ sensor synaptotagmin VII to lysosomes. J Cell Biol. 2010;191:599-613 pubmed publisher..These findings identify palmitoylation-dependent association with the tetraspanin CD63 as the mechanism by which Syt VII is targeted to lysosomes. ..
- Xiao Y, Lu Y, Heu S, Hutcheson S. Organization and environmental regulation of the Pseudomonas syringae pv. syringae 61 hrp cluster. J Bacteriol. 1992;174:1734-41 pubmed..syringae 61 hrp genes at levels exceeding 0.028%. The results indicate that enhanced expression of hrp genes occurs early in the interaction with nonhost plant species in an apparent response to altered nutritional conditions...
- McCann C, Kriebel P, Parent C, Losert W. Cell speed, persistence and information transmission during signal relay and collective migration. J Cell Sci. 2010;123:1724-31 pubmed publisher..We envision that signal relay has a similar key role in the migration of a variety of chemotaxing mammalian cells that can relay chemoattractant signals. ..
- Higgins S, Ellestad L, Trakooljul N, McCarthy F, Saliba J, Cogburn L, et al. Transcriptional and pathway analysis in the hypothalamus of newly hatched chicks during fasting and delayed feeding. BMC Genomics. 2010;11:162 pubmed publisher..Considering that POMC, an important gene in controlling metabolism, was central to this network, this gene network may play an important role in regulation of feeding and metabolism in birds. ..
- Bretz J, Mock N, Charity J, Zeyad S, Baker C, Hutcheson S. A translocated protein tyrosine phosphatase of Pseudomonas syringae pv. tomato DC3000 modulates plant defence response to infection. Mol Microbiol. 2003;49:389-400 pubmed..The results indicate that HopPtoD2 is a translocated effector with protein tyrosine phosphatase activity that modulates plant defence responses. ..
- Lorber B, Ades I. Regulation of biogenesis of liver delta-aminolevulinate synthase: effects of structural modifications of heme on the enzyme's RNA. Int J Biochem. 1989;21:439-43 pubmed..5. In conclusion, the effect of a given metallo-porphyrin on liver ALA synthase RNA following side chain modification depended on the coordinated metal...