Experts and Doctors on mutation in Ames, Iowa, United States


Locale: Ames, Iowa, United States
Topic: mutation

Top Publications

  1. Kirthi N, Roy Chaudhuri B, Kelley T, Culver G. A novel single amino acid change in small subunit ribosomal protein S5 has profound effects on translational fidelity. RNA. 2006;12:2080-91 pubmed
    ..This study highlights the importance of S5 in ribosome function and cell physiology, and suggests that translational fidelity can be regulated in multiple ways. ..
  2. Edgington N, Blacketer M, Bierwagen T, Myers A. Control of Saccharomyces cerevisiae filamentous growth by cyclin-dependent kinase Cdc28. Mol Cell Biol. 1999;19:1369-80 pubmed
    ..The data suggest that a protein kinase cascade involving Elm1, Hsl1, and Swe1 can modulate Cdc28 activity and that Cdc28 in turn exerts global effects that cause filamentous growth. ..
  3. Li Y, Cai W, Wang C, Yao C, Bao X, Deng H, et al. Domain requirements of the JIL-1 tandem kinase for histone H3 serine 10 phosphorylation and chromatin remodeling in vivo. J Biol Chem. 2013;288:19441-9 pubmed publisher
  4. Devkota S, Joseph R, Boyken S, Fulton D, Andreotti A. An Autoinhibitory Role for the Pleckstrin Homology Domain of Interleukin-2-Inducible Tyrosine Kinase and Its Interplay with Canonical Phospholipid Recognition. Biochemistry. 2017;56:2938-2949 pubmed publisher
  5. Xu M, Brar H, Grosic S, Palmer R, Bhattacharyya M. Excision of an active CACTA-like transposable element from DFR2 causes variegated flowers in soybean [Glycine max (L.) Merr.]. Genetics. 2010;184:53-63 pubmed publisher
    ..The highly active endogenous transposon, Tgm9, should facilitate genomics studies specifically that relate to legume biology. ..
  6. Xu Y, Zhang F, Su Z, McNew J, Shin Y. Hemifusion in SNARE-mediated membrane fusion. Nat Struct Mol Biol. 2005;12:417-22 pubmed
    ..Hemifusion was also detected with wild-type SNAREs when low protein concentrations were reconstituted. Thus, these results show that SNARE-mediated fusion can transit through a hemifusion intermediate. ..
  7. Skaff D, Kim C, Tsai H, Honzatko R, Fromm H. Glucose 6-phosphate release of wild-type and mutant human brain hexokinases from mitochondria. J Biol Chem. 2005;280:38403-9 pubmed
    ..Results here implicate a primary role for the glucose 6-phosphate binding site at the N-terminal half of HKI in the release mechanism. ..
  8. Wang L, Vinogradov E, Bogdanove A. Requirement of the lipopolysaccharide O-chain biosynthesis gene wxocB for type III secretion and virulence of Xanthomonas oryzae pv. Oryzicola. J Bacteriol. 2013;195:1959-69 pubmed publisher
    ..Thus, virulence of the wxocB mutant is likely reduced due primarily to a direct, possibly structural, effect of the loss of the O chain on type III delivery of effector proteins. ..
  9. Chang C, Yoon K, Zimmerman J, Harmon K, Dixon P, Dvorak C, et al. Evolution of porcine reproductive and respiratory syndrome virus during sequential passages in pigs. J Virol. 2002;76:4750-63 pubmed
    ..Although no epitopic changes were detected under the conditions of this experiment, PRRS virus was shown to evolve continuously in infected pigs, with different genes of the viral genome undergoing various degrees of change. ..

More Information


  1. Gao H, Narayanan N, Ellison L, Bhattacharyya M. Two classes of highly similar coiled coil-nucleotide binding-leucine rich repeat genes isolated from the Rps1-k locus encode Phytophthora resistance in soybean. Mol Plant Microbe Interact. 2005;18:1035-45 pubmed
    ..Analyses of independent R0, R1, R2, and R3 progeny populations suggested that both gene classes confer Phytophthora resistance in soybean. A possible evolutionary mechanism for the Class I gene family is proposed. ..
  2. Liu F, Cui X, Horner H, Weiner H, Schnable P. Mitochondrial aldehyde dehydrogenase activity is required for male fertility in maize. Plant Cell. 2001;13:1063-78 pubmed
    ..Indeed, the RF2 protein is capable of oxidizing at least three aldehydes. Hence, the specific metabolic pathway(s) within which the rf2-encoded mtALDH acts remains to be discovered. ..
  3. Nowling T, Zhou W, Krieger K, Larochelle C, Nguyen Huu M, Jeannotte L, et al. Hoxa5 gene regulation: A gradient of binding activity to a brachial spinal cord element. Dev Biol. 1999;208:134-46 pubmed
    ..The brachial spinal cord element and its binding proteins are likely to be involved in spatial expression of Hoxa5 during development. ..
  4. Wu D, Wright D, Wetzel C, Voytas D, Rodermel S. The IMMUTANS variegation locus of Arabidopsis defines a mitochondrial alternative oxidase homolog that functions during early chloroplast biogenesis. Plant Cell. 1999;11:43-55 pubmed
  5. Zheng H, McKay J, Buss J. H-Ras does not need COP I- or COP II-dependent vesicular transport to reach the plasma membrane. J Biol Chem. 2007;282:25760-8 pubmed
    ..Thus, H-Ras has two simultaneous but distinct means of transport and need not depend on vesicular trafficking for its delivery to the plasma membrane. ..
  6. Cai W, Bao X, Deng H, Jin Y, Girton J, Johansen J, et al. RNA polymerase II-mediated transcription at active loci does not require histone H3S10 phosphorylation in Drosophila. Development. 2008;135:2917-25 pubmed publisher
  7. Bao X, Cai W, Deng H, Zhang W, Krencik R, Girton J, et al. The COOH-terminal domain of the JIL-1 histone H3S10 kinase interacts with histone H3 and is required for correct targeting to chromatin. J Biol Chem. 2008;283:32741-50 pubmed publisher
    ..Taken together these findings indicate that JIL-1 may participate in regulating chromatin structure by multiple and partially redundant mechanisms. ..
  8. Singh R. Evolving concepts on human SMN pre-mRNA splicing. RNA Biol. 2007;4:7-10 pubmed
    ..Considering an antisense-based strategy confers a unique advantage of sequence specificity, availability of many target worthy cis-elements holds strong potential for antisense-mediated therapy of SMA. ..
  9. Wen T, Hochholdinger F, Sauer M, Bruce W, Schnable P. The roothairless1 gene of maize encodes a homolog of sec3, which is involved in polar exocytosis. Plant Physiol. 2005;138:1637-43 pubmed publisher
    ..The preferential accumulation in the rth1 mutant proteome of a negative regulator of the cell cycle (a prohibitin) may at least partially explain the delayed development and flowering of the rth1 mutant...
  10. Zabotina O, Avci U, Cavalier D, Pattathil S, Chou Y, Eberhard S, et al. Mutations in multiple XXT genes of Arabidopsis reveal the complexity of xyloglucan biosynthesis. Plant Physiol. 2012;159:1367-84 pubmed publisher
  11. Dietrich C, Cui F, Packila M, Li J, Ashlock D, Nikolau B, et al. Maize Mu transposons are targeted to the 5' untranslated region of the gl8 gene and sequences flanking Mu target-site duplications exhibit nonrandom nucleotide composition throughout the genome. Genetics. 2002;160:697-716 pubmed
    ..Hence, this study provides strong evidence for the involvement of sequences flanking the TSD in Mu insertion-site selection. ..
  12. Radke K, Johnson K, Guo R, Davidson A, Ambrosio L. Drosophila-raf acts to elaborate dorsoventral pattern in the ectoderm of developing embryos. Genetics. 2001;159:1031-44 pubmed
    ..The consequences of eliminating key D-raf regulatory domains and specific serine residues in the transmission of Egfr and lateral epidermal signals were also addressed here. ..
  13. Soto Burgos J, Bassham D. SnRK1 activates autophagy via the TOR signaling pathway in Arabidopsis thaliana. PLoS ONE. 2017;12:e0182591 pubmed publisher
    ..Furthermore, an increase in SnRK1 activity was unable to induce autophagy when TOR was also activated. These results demonstrate that SnRK1 acts upstream of TOR in the activation of autophagy in Arabidopsis. ..
  14. Li Y, Wang C, Cai W, Sengupta S, Zavortink M, Deng H, et al. H2Av facilitates H3S10 phosphorylation but is not required for heat shock-induced chromatin decondensation or transcriptional elongation. Development. 2017;144:3232-3240 pubmed publisher
    ..These data strongly suggest that H2Av or H3S10 phosphorylation by JIL-1 is not required for chromatin decondensation or transcriptional elongation in Drosophila. ..
  15. Luangtongkum T, Shen Z, Seng V, Sahin O, Jeon B, Liu P, et al. Impaired fitness and transmission of macrolide-resistant Campylobacter jejuni in its natural host. Antimicrob Agents Chemother. 2012;56:1300-8 pubmed publisher
    ..jejuni will likely decrease in the absence of antibiotic selection pressure...
  16. Yu F, Liu X, Alsheikh M, Park S, Rodermel S. Mutations in SUPPRESSOR OF VARIEGATION1, a factor required for normal chloroplast translation, suppress var2-mediated leaf variegation in Arabidopsis. Plant Cell. 2008;20:1786-804 pubmed publisher
    ..Our data indicate that defects in chloroplast rRNA processing are a common, but not universal, molecular phenotype associated with suppression of var2 variegation. ..
  17. Fan C, Bobik T. The PduX enzyme of Salmonella enterica is an L-threonine kinase used for coenzyme B12 synthesis. J Biol Chem. 2008;283:11322-9 pubmed publisher
    ..To our knowledge, PduX is the first enzyme shown to phosphorylate free L-threonine and the first L-threonine kinase shown to function in coenzyme B(12) synthesis. ..
  18. Wang Z, Treder K, Miller W. Structure of a viral cap-independent translation element that functions via high affinity binding to the eIF4E subunit of eIF4F. J Biol Chem. 2009;284:14189-202 pubmed publisher
    ..We conclude that the PTE recruits eIF4F by binding eIF4E. The PTE represents a different class of translation enhancer element, as defined by its structure and ability to bind eIF4E in the absence of an m(7)G cap...
  19. Laederach A, Cradic K, Brazin K, Zamoon J, Fulton D, Huang X, et al. Competing modes of self-association in the regulatory domains of Bruton's tyrosine kinase: intramolecular contact versus asymmetric homodimerization. Protein Sci. 2002;11:36-45 pubmed
  20. Ross J, Fernandez de Castro J, Zhao J, Samuel M, Walters E, Rios C, et al. Generation of an inbred miniature pig model of retinitis pigmentosa. Invest Ophthalmol Vis Sci. 2012;53:501-7 pubmed publisher
    ..This large-animal model can serve as a novel tool for the study of the pathogenesis and therapeutic intervention in the most common form of adRP...
  21. Huang Z, Yeakley J, Garcia E, Holdridge J, Fan J, Whitham S. Salicylic acid-dependent expression of host genes in compatible Arabidopsis-virus interactions. Plant Physiol. 2005;137:1147-59 pubmed
  22. PLUMMER P, Sahin O, Burrough E, Sippy R, Mou K, Rabenold J, et al. Critical role of LuxS in the virulence of Campylobacter jejuni in a guinea pig model of abortion. Infect Immun. 2012;80:585-93 pubmed publisher
    ..These studies provide the first direct evidence that LuxS plays an important role in the virulence of C. jejuni using an in vivo model of natural disease...
  23. Reinhardt T, Lippolis J, Shull G, Horst R. Null mutation in the gene encoding plasma membrane Ca2+-ATPase isoform 2 impairs calcium transport into milk. J Biol Chem. 2004;279:42369-73 pubmed
    ..This major secretory function represents a novel biological role for the plasma membrane Ca2+-ATPases, which are generally regarded as premier regulators of intracellular Ca2+. ..
  24. Chen H, Rosin F, Prat S, Hannapel D. Interacting transcription factors from the three-amino acid loop extension superclass regulate tuber formation. Plant Physiol. 2003;132:1391-404 pubmed
    ..Our results demonstrate an interaction between KNOX and BEL1-like transcription factors of potato that may potentially regulate processes of development...
  25. Kim S, Bassham D. TNO1 is involved in salt tolerance and vacuolar trafficking in Arabidopsis. Plant Physiol. 2011;156:514-26 pubmed publisher
    ..These data demonstrate that TNO1 is involved in vacuolar trafficking and salt tolerance, potentially via roles in vesicle fusion and in maintaining TGN structure or identity. ..
  26. Shao Z, Zhang Y, Ye Q, Saldanha J, Powell Coffman J. C. elegans SWAN-1 Binds to EGL-9 and regulates HIF-1-mediated resistance to the bacterial pathogen Pseudomonas aeruginosa PAO1. PLoS Pathog. 2010;6:e1001075 pubmed publisher
    ..aeruginosa. These data support a model in which SWAN-1, MBK-1 and EGL-9 regulate HIF-1 transcriptional activity and modulate resistance to P. aeruginosa PAO1 fast killing. ..
  27. Jia Y, Lisch D, Ohtsu K, Scanlon M, Nettleton D, Schnable P. Loss of RNA-dependent RNA polymerase 2 (RDR2) function causes widespread and unexpected changes in the expression of transposons, genes, and 24-nt small RNAs. PLoS Genet. 2009;5:e1000737 pubmed publisher
  28. Bannantine J, Bermudez L. No holes barred: invasion of the intestinal mucosa by Mycobacterium avium subsp. paratuberculosis. Infect Immun. 2013;81:3960-5 pubmed publisher
    ..These notable findings are summarized along with added mechanistic details of the early infection model. We conclude by proposing critical next steps to further elucidate the process of M. avium subsp. paratuberculosis invasion. ..
  29. Fu A, Park S, Rodermel S. Sequences required for the activity of PTOX (IMMUTANS), a plastid terminal oxidase: in vitro and in planta mutagenesis of iron-binding sites and a conserved sequence that corresponds to Exon 8. J Biol Chem. 2005;280:42489-96 pubmed
    ..Chloroplast import assays revealed that mutant enzymes lacking Exon 8 have enhanced turnover. We conclude that the Exon 8 domain is required not only for PTOX activity but also for its stability. ..
  30. Dinges J, Colleoni C, James M, Myers A. Mutational analysis of the pullulanase-type debranching enzyme of maize indicates multiple functions in starch metabolism. Plant Cell. 2003;15:666-80 pubmed
    ..Therefore, pullulanase-type DBE partially compensates for the defect in isoamylase-type DBE, suggesting a function during starch synthesis as well as degradation. ..
  31. Srivastava R, Deng Y, Shah S, Rao A, Howell S. BINDING PROTEIN is a master regulator of the endoplasmic reticulum stress sensor/transducer bZIP28 in Arabidopsis. Plant Cell. 2013;25:1416-29 pubmed publisher
    ..Thus, the dissociation of BiP from the C-terminal tail of bZIP28 is a major switch that activates one arm of the unfolded protein response signaling pathway in plants. ..
  32. Yang Q, He Y, Kabahuma M, Chaya T, Kelly A, Borrego E, et al. A gene encoding maize caffeoyl-CoA O-methyltransferase confers quantitative resistance to multiple pathogens. Nat Genet. 2017;49:1364-1372 pubmed publisher
  33. Wang W, Gorrell A, Honzatko R, Fromm H. A study of Escherichia coli adenylosuccinate synthetase association states and the interface residues of the homodimer. J Biol Chem. 1997;272:7078-84 pubmed
    ..Our results suggest that IMP binding induces enzyme dimerization and that two residues in the interface region, Arg143 and Asp231, play significant roles in IMP and GTP binding. ..
  34. Han J, Sahin O, Barton Y, Zhang Q. Key role of Mfd in the development of fluoroquinolone resistance in Campylobacter jejuni. PLoS Pathog. 2008;4:e1000083 pubmed publisher
  35. Liu S, Yeh C, Tang H, Nettleton D, Schnable P. Gene mapping via bulked segregant RNA-Seq (BSR-Seq). PLoS ONE. 2012;7:e36406 pubmed publisher
    ..The gl3 gene encodes a putative myb transcription factor, which directly or indirectly affects the expression of a number of genes involved in the biosynthesis of very-long-chain fatty acids. ..
  36. De la Rosa M, Nelson S. An interaction between the Walker A and D-loop motifs is critical to ATP hydrolysis and cooperativity in bacteriophage T4 Rad50. J Biol Chem. 2011;286:26258-66 pubmed publisher
    ..Additionally, we propose that the asparagine acts as a ?-phosphate sensor and, through its interaction with the conserved D-loop aspartate, transmits conformational changes across the dimer interface to the second ATP binding site...
  37. Li X, Ilarslan H, Brachova L, Qian H, Li L, Che P, et al. Reverse-genetic analysis of the two biotin-containing subunit genes of the heteromeric acetyl-coenzyme A carboxylase in Arabidopsis indicates a unidirectional functional redundancy. Plant Physiol. 2011;155:293-314 pubmed publisher
  38. Yu F, Park S, Rodermel S. Functional redundancy of AtFtsH metalloproteases in thylakoid membrane complexes. Plant Physiol. 2005;138:1957-66 pubmed
  39. Shao Z, Zhang Y, Powell Coffman J. Two distinct roles for EGL-9 in the regulation of HIF-1-mediated gene expression in Caenorhabditis elegans. Genetics. 2009;183:821-9 pubmed publisher
  40. Yi G, Lauter A, Scott M, Becraft P. The thick aleurone1 mutant defines a negative regulation of maize aleurone cell fate that functions downstream of defective kernel1. Plant Physiol. 2011;156:1826-36 pubmed publisher
    ..The thk1 mutation was found to be caused by a deletion of approximately 2 megabases. ..
  41. Deng H, Cai W, Wang C, Lerach S, Delattre M, Girton J, et al. JIL-1 and Su(var)3-7 interact genetically and counteract each other's effect on position-effect variegation in Drosophila. Genetics. 2010;185:1183-92 pubmed publisher
    ..These observations suggest a model where Su(var)3-7 functions as an effector downstream of Su(var)3-9 and H3K9 dimethylation in heterochromatic spreading and gene silencing that is normally counteracted by JIL-1 kinase activity. ..
  42. Maxson J, Luty S, Macmaniman J, Paik J, Gotlib J, Greenberg P, et al. The Colony-Stimulating Factor 3 Receptor T640N Mutation Is Oncogenic, Sensitive to JAK Inhibition, and Mimics T618I. Clin Cancer Res. 2016;22:757-64 pubmed publisher
    ..Because of its similarities to CSF3R T618I, the T640N mutation likely has diagnostic and therapeutic relevance in CNL/aCML. ..
  43. Johansen K, Johansen J. Regulation of chromatin structure by histone H3S10 phosphorylation. Chromosome Res. 2006;14:393-404 pubmed
  44. Gao M, Wanat J, Stinard P, James M, Myers A. Characterization of dull1, a maize gene coding for a novel starch synthase. Plant Cell. 1998;10:399-412 pubmed
    ..We conclude that Du1 codes for a starch synthase, most likely SSII, and that secondary effects of du1- mutations, such as reduction of SBEIIa, result from the primary deficiency in this starch synthase. ..
  45. Kang C, Kim S, Fromm H. Subunit complementation of Escherichia coli adenylosuccinate synthetase. J Biol Chem. 1996;271:29722-8 pubmed
    ..9 microM, respectively. A concentration dependence of the specific activity of the wild-type protein in this study shows that the Kd for dimer dissociation is approximately 1 microM. ..