Experts and Doctors on cho cells in Iowa City, Iowa, United States


Locale: Iowa City, Iowa, United States
Topic: cho cells

Top Publications

  1. Eppinga R, Li Y, Lin J, Lin J. Tropomyosin and caldesmon regulate cytokinesis speed and membrane stability during cell division. Arch Biochem Biophys. 2006;456:161-74 pubmed
    ..These results support a model where Ca(2+)/calmodulin and cdk1 dynamically control caldesmon inhibition of tropomyosin-activated actomyosin to regulate division speed and to suppress membrane blebs. ..
  2. O Hara S, Lin J. Accumulation of tropomyosin isoform 5 at the infection sites of host cells during Cryptosporidium invasion. Parasitol Res. 2006;99:45-54 pubmed
    ..Cryptosporidium infection appears to rearrange and recruit host TM isoforms in both culture cells and in the mouse. Localized accumulation of tropomyosin at the infection sites may facilitate parasite invasion. ..
  3. Stoltz D, Ozer E, Ng C, Yu J, Reddy S, Lusis A, et al. Paraoxonase-2 deficiency enhances Pseudomonas aeruginosa quorum sensing in murine tracheal epithelia. Am J Physiol Lung Cell Mol Physiol. 2007;292:L852-60 pubmed
    ..In summary, these results show that loss of PON2 impairs 3OC12-HSL degradation by airway epithelial cells and suggests that diffusion of 3OC12-HSL into the airway cells can be the rate-limiting step for degradation of the molecule. ..
  4. Gode Potratz C, Chodur D, McCarter L. Calcium and iron regulate swarming and type III secretion in Vibrio parahaemolyticus. J Bacteriol. 2010;192:6025-38 pubmed publisher
    ..Thus, calcium and iron, two ions pertinent for a marine organism and pathogen, play a signaling role with global consequences on the regulation of gene sets that are relevant for surface colonization and infection. ..
  5. Ashbourne Excoffon K, Moninger T, Zabner J. The coxsackie B virus and adenovirus receptor resides in a distinct membrane microdomain. J Virol. 2003;77:2559-67 pubmed
    ..Residence in a lipid-rich domain provides a mechanism that allows CAR to interact with other cell adhesion proteins and yet function as an adenovirus receptor. ..
  6. Warren K, Lin J, McDermott J, Lin J. Forced expression of chimeric human fibroblast tropomyosin mutants affects cytokinesis. J Cell Biol. 1995;129:697-708 pubmed
    ..This suggests that regulation of microfilament integrity in vivo requires stabilizing factors other than, or in addition to, TM. ..
  7. Warren K, Lin J, Wamboldt D, Lin J. Overexpression of human fibroblast caldesmon fragment containing actin-, Ca++/calmodulin-, and tropomyosin-binding domains stabilizes endogenous tropomyosin and microfilaments. J Cell Biol. 1994;125:359-68 pubmed
    ..Therefore, it is likely that CaD39 can enhance TM's binding to F-actin in vivo, thus reducing TM's rate of turnover and stabilizing actin microfilament bundles. ..
  8. Ahluwalia J, Topp J, Weirather K, Zimmerman M, Stamnes M. A role for calcium in stabilizing transport vesicle coats. J Biol Chem. 2001;276:34148-55 pubmed
    ..The primary target for the effects of calcium on coatomer recruitment is a step that occurs after ADP-ribosylation factor binding to the membrane. We suggest that a calcium gradient may serve to regulate the timing of vesicle uncoating. ..
  9. Kanzaki M, Watson R, Hou J, Stamnes M, Saltiel A, Pessin J. Small GTP-binding protein TC10 differentially regulates two distinct populations of filamentous actin in 3T3L1 adipocytes. Mol Biol Cell. 2002;13:2334-46 pubmed
    ..Together, these data demonstrate that TC10 can differentially regulate two types of filamentous actin in adipocytes dependent on distinct functional domains and its subcellular compartmentalization. ..

More Information


  1. Li Y, Wessels D, Wang T, Lin J, Soll D, Lin J. Regulation of caldesmon activity by Cdc2 kinase plays an important role in maintaining membrane cortex integrity during cell division. Cell Mol Life Sci. 2003;60:198-211 pubmed
    ..These results suggest that mitosis-specific phosphorylation of CaD plays a role in inhibiting bleb formation and that the N-terminal fragment of CaD is required for cell size determination. ..
  2. Eppinga R, Peng I, Lin J, Wu C, Lin J. Opposite effects of overexpressed myosin Va or heavy meromyosin Va on vesicle distribution, cytoskeleton organization, and cell motility in nonmuscle cells. Cell Motil Cytoskeleton. 2008;65:197-215 pubmed
    ..Together, these data suggest that myosin Va overexpression induces actin bundles in vivo whereas the tailless version fails to bundle actin and disrupts cell motility. ..
  3. Wood J, Russo A. Autoregulation of cell-specific MAP kinase control of the tryptophan hydroxylase promoter. J Biol Chem. 2001;276:21262-71 pubmed
    ..These data suggest a model for the autoregulation of serotonin biosynthesis by repression of MAP kinase stimulation of the TPH promoter. ..
  4. Stoltz D, Ozer E, Recker T, Estin M, Yang X, Shih D, et al. A common mutation in paraoxonase-2 results in impaired lactonase activity. J Biol Chem. 2009;284:35564-71 pubmed publisher
    ..These data suggest that impaired lactonase activity may play a role in innate immunity, atherosclerosis, and other diseases associated with the PON2 311 SNP. ..
  5. Inamori K, Hara Y, Willer T, Anderson M, Zhu Z, Yoshida Moriguchi T, et al. Xylosyl- and glucuronyltransferase functions of LARGE in ?-dystroglycan modification are conserved in LARGE2. Glycobiology. 2013;23:295-302 pubmed publisher
    ..Our results suggest that LARGE and LARGE2 catalyze the same glycosylation reactions for the functional modification of ?-DG, but that they have different biochemical properties. ..
  6. Toro R, Saadi I, Kuburas A, Nemer M, Russo A. Cell-specific activation of the atrial natriuretic factor promoter by PITX2 and MEF2A. J Biol Chem. 2004;279:52087-94 pubmed
    ..These results demonstrate a promoter- and cell-specific functional interaction between PITX2 and MEF2A and suggest the possibility of coordinate control by these factors in the oral epithelium. ..
  7. Nakano Y, Banfi B, Jesaitis A, Dinauer M, Allen L, Nauseef W. Critical roles for p22phox in the structural maturation and subcellular targeting of Nox3. Biochem J. 2007;403:97-108 pubmed
    ..Taken together, these studies demonstrate critical roles for p22phox at several distinct points in the maturation and assembly of a functionally competent Nox3 in the plasma membrane. ..
  8. Dayal D, Martin S, Owens K, Aykin Burns N, Zhu Y, Boominathan A, et al. Mitochondrial complex II dysfunction can contribute significantly to genomic instability after exposure to ionizing radiation. Radiat Res. 2009;172:737-45 pubmed publisher
    ..These results support the hypothesis that mitochondrial dysfunction originating from complex II can contribute to radiation-induced genomic instability by increasing steady-state levels of reactive oxygen species. ..
  9. Li Y, Lin J, Reiter R, Daniels K, Soll D, Lin J. Caldesmon mutant defective in Ca(2+)-calmodulin binding interferes with assembly of stress fibers and affects cell morphology, growth and motility. J Cell Sci. 2004;117:3593-604 pubmed
    ..Together, these results demonstrate that CaD plays a crucial role in mediating the effects of Ca(2+)-CaM on the dynamics of the actin cytoskeleton during cell migration. ..
  10. Excoffon K, Traver G, Zabner J. The role of the extracellular domain in the biology of the coxsackievirus and adenovirus receptor. Am J Respir Cell Mol Biol. 2005;32:498-503 pubmed
    ..Surprisingly, the D2 domain was required for adenovirus fiber-knob binding and infection. In summary, the entire extracellular domain of CAR is of vital importance to the biology of this highly conserved and important protein...
  11. Wang Q, Lu T, Adams E, Lin J, Lin J. Intercalated disc protein, mXin?, suppresses p120-catenin-induced branching phenotype via its interactions with p120-catenin and cortactin. Arch Biochem Biophys. 2013;535:91-100 pubmed publisher
    ..It is known that p120-catenin directly regulates Rho GTPases, leading to the branching phenotype. Thus, mXin? may sequester the p120-catenin from inhibiting RhoA activity and/or from activating Rac1 activity. ..
  12. Chatterjee T, Sharma R, Fisher R. Molecular cloning of a novel variant of the pituitary adenylate cyclase-activating polypeptide (PACAP) receptor that stimulates calcium influx by activation of L-type calcium channels. J Biol Chem. 1996;271:32226-32 pubmed
  13. Okada S, Pessin J. Interactions between Src homology (SH) 2/SH3 adapter proteins and the guanylnucleotide exchange factor SOS are differentially regulated by insulin and epidermal growth factor. J Biol Chem. 1996;271:25533-8 pubmed
  14. Saadi I, Kuburas A, Engle J, Russo A. Dominant negative dimerization of a mutant homeodomain protein in Axenfeld-Rieger syndrome. Mol Cell Biol. 2003;23:1968-82 pubmed
    ..These results suggest that the position 50 residue in the PITX2 homeodomain plays an important role in both DNA binding and dimerization activities. ..
  15. Waters S, Yamauchi K, Pessin J. Insulin-stimulated disassociation of the SOS-Grb2 complex. Mol Cell Biol. 1995;15:2791-9 pubmed
    ..Together, these data suggest a molecular mechanism accounting for the transient activation of ras due to the uncoupling of the SOS-Grb2 complex following SOS phosphorylation. ..
  16. Urbanowski M, Brutinel E, Yahr T. Translocation of ExsE into Chinese hamster ovary cells is required for transcriptional induction of the Pseudomonas aeruginosa type III secretion system. Infect Immun. 2007;75:4432-9 pubmed
  17. Thompson A, Nessler R, Wisco D, Anderson E, Winckler B, Sheff D. Recycling endosomes of polarized epithelial cells actively sort apical and basolateral cargos into separate subdomains. Mol Biol Cell. 2007;18:2687-97 pubmed
    ..This suggests that subdomain and transport vesicle formation occur as separate sorting steps and that both processes may contribute to sorting fidelity. ..
  18. Eppinga R, Li Y, Lin J, Mak A, Lin J. Requirement of reversible caldesmon phosphorylation at P21-activated kinase-responsive sites for lamellipodia extensions during cell migration. Cell Motil Cytoskeleton. 2006;63:543-62 pubmed
    ..We conclude that phosphorylation of caldesmon by PAK is a dynamic process required to regulate actin dynamics and membrane protrusions in wound-induced cell migration. ..
  19. Harding W, Tidgewell K, Byrd N, Cobb H, Dersch C, Butelman E, et al. Neoclerodane diterpenes as a novel scaffold for mu opioid receptor ligands. J Med Chem. 2005;48:4765-71 pubmed
    ..This represents the identification of a novel structural class of mu opioid receptor agonists. ..
  20. Price M, Thompson R, Eshcol J, Wemmie J, Benson C. Stomatin modulates gating of acid-sensing ion channels. J Biol Chem. 2004;279:53886-91 pubmed
    ..Our findings indicate that modulation of DEG/ENaC channels by stomatin-like proteins is evolutionarily conserved and may have important implications for mammalian nociception and mechanosensation. ..
  21. Hruska Hageman A, Benson C, Leonard A, Price M, Welsh M. PSD-95 and Lin-7b interact with acid-sensing ion channel-3 and have opposite effects on H+- gated current. J Biol Chem. 2004;279:46962-8 pubmed
    ..The finding that multiple PDZ-containing proteins bind ASIC3 and can influence its presence in the plasma membrane suggests that they may play an important role in the contribution of ASIC3 to nociception and mechanosensation. ..
  22. Pessin J, Okada S. Insulin and EGF receptors integrate the Ras and Rap signaling pathways. Endocr J. 1999;46 Suppl:S11-6 pubmed
    ..Thus, efficient regulation of receptor tyrosine kinase downstream signaling events require the coordinate interplay of these two pathways. ..
  23. Inamori K, Yoshida Moriguchi T, Hara Y, Anderson M, Yu L, Campbell K. Dystroglycan function requires xylosyl- and glucuronyltransferase activities of LARGE. Science. 2012;335:93-6 pubmed publisher
    ..This modification allowed ?-DG to bind laminin-G domain-containing ECM ligands. ..
  24. Excoffon K, Avenarius M, Hansen M, Kimberling W, Najmabadi H, Smith R, et al. The Coxsackievirus and Adenovirus Receptor: a new adhesion protein in cochlear development. Hear Res. 2006;215:1-9 pubmed
  25. Eshcol J, Harding A, Hattori T, Costa V, Welsh M, Benson C. Acid-sensing ion channel 3 (ASIC3) cell surface expression is modulated by PSD-95 within lipid rafts. Am J Physiol Cell Physiol. 2008;295:C732-9 pubmed publisher
    ..In addition, we found that cell surface ASIC3 is enriched in the lipid raft fraction. These data suggest that PSD-95 and ASIC3 interact within lipid rafts and that this raft interaction is required for PSD-95 to modulate ASIC3. ..
  26. Kao A, Waters S, Okada S, Pessin J. Insulin stimulates the phosphorylation of the 66- and 52-kilodalton Shc isoforms by distinct pathways. Endocrinology. 1997;138:2474-80 pubmed
    ..These data demonstrate that insulin stimulates the serine phosphorylation of the 66-kDa Shc isoform through a MEK-dependent mechanism. ..
  27. Kusama N, Gautam M, Harding A, Snyder P, Benson C. Acid-sensing ion channels (ASICs) are differentially modulated by anions dependent on their subunit composition. Am J Physiol Cell Physiol. 2013;304:C89-101 pubmed publisher
    ..We speculate that modulation of ASIC gating by Cl(-) is a novel mechanism to sense shifts in extracellular fluid composition. ..
  28. Okada S, Kao A, Ceresa B, Blaikie P, Margolis B, Pessin J. The 66-kDa Shc isoform is a negative regulator of the epidermal growth factor-stimulated mitogen-activated protein kinase pathway. J Biol Chem. 1997;272:28042-9 pubmed
  29. Walder R, Gautam M, Wilson S, Benson C, Sluka K. Selective targeting of ASIC3 using artificial miRNAs inhibits primary and secondary hyperalgesia after muscle inflammation. Pain. 2011;152:2348-56 pubmed publisher
  30. Okada S, Matsuda M, Anafi M, Pawson T, Pessin J. Insulin regulates the dynamic balance between Ras and Rap1 signaling by coordinating the assembly states of the Grb2-SOS and CrkII-C3G complexes. EMBO J. 1998;17:2554-65 pubmed
    ..Together, these data demonstrate that insulin treatment results in the de-repression of Rap1 inhibitory function on the Raf1 kinase concomitant with Ras activation and stimulation of the downstream Raf1/MEK/ERK cascade. ..
  31. Schnizler M, Schnizler K, Zha X, Hall D, Wemmie J, Hell J, et al. The cytoskeletal protein alpha-actinin regulates acid-sensing ion channel 1a through a C-terminal interaction. J Biol Chem. 2009;284:2697-705 pubmed publisher
    ..These findings suggest that alpha-actinins may link ASIC1a to a macromolecular complex in the postsynaptic membrane where it regulates ASIC1a activity. ..
  32. Choi S, Gustafson Wagner E, Wang Q, Harlan S, Sinn H, Lin J, et al. The intercalated disk protein, mXinalpha, is capable of interacting with beta-catenin and bundling actin filaments [corrected]. J Biol Chem. 2007;282:36024-36 pubmed
    ..In support of this model, we found that the actin binding and bundling activity of mXinalpha was enhanced in the presence of beta-catenin. ..
  33. Simpson D, Katavic P, Lozama A, Harding W, Parrish D, Deschamps J, et al. Synthetic studies of neoclerodane diterpenes from Salvia divinorum: preparation and opioid receptor activity of salvinicin analogues. J Med Chem. 2007;50:3596-603 pubmed
    ..This indicates that additional structural modifications of 1a may lead to analogues with higher potency and utility as drug abuse medications. ..
  34. Seiler M, Miller A, Zabner J, Halbert C. Adeno-associated virus types 5 and 6 use distinct receptors for cell entry. Hum Gene Ther. 2006;17:10-9 pubmed
  35. Harding W, Schmidt M, Tidgewell K, Kannan P, Holden K, Dersch C, et al. Synthetic studies of neoclerodane diterpenes from Salvia divinorum: selective modification of the furan ring. Bioorg Med Chem Lett. 2006;16:3170-4 pubmed
    ..In addition, a procedure has been found to remove the furan skeleton completely. Biological results indicate that replacement of the furan ring with an N-sulfonylpyrrole leads to reduced affinity and efficacy at kappa opioid receptors...
  36. Brown K, Hostager B, Bishop G. Regulation of TRAF2 signaling by self-induced degradation. J Biol Chem. 2002;277:19433-8 pubmed
    ..Several approaches show that ubiquitination and proteasomal activity are integral to TRAF2 degradation, and inhibition of this process potentiates CD40 signaling. ..
  37. Crosbie R, Lebakken C, Holt K, Venzke D, Straub V, Lee J, et al. Membrane targeting and stabilization of sarcospan is mediated by the sarcoglycan subcomplex. J Cell Biol. 1999;145:153-65 pubmed
    ..In addition, the SG- SPN subcomplex functions to stabilize alpha-dystroglycan to the muscle plasma membrane. Taken together, our data provide important information about assembly and function of the SG-SPN subcomplex. ..
  38. Saadi I, Toro R, Kuburas A, Semina E, Murray J, Russo A. An unusual class of PITX2 mutations in Axenfeld-Rieger syndrome. Birth Defects Res A Clin Mol Teratol. 2006;76:175-81 pubmed
    ..The results indicate that the PITX2 C-terminal domain has inhibitory activity and support the notion that ARS may also be caused by gain-of-function mutations. ..