Experts and Doctors on phosphates in West Lafayette, Indiana, United States


Locale: West Lafayette, Indiana, United States
Topic: phosphates

Top Publications

  1. Hsieh Y, Wanner B. Global regulation by the seven-component Pi signaling system. Curr Opin Microbiol. 2010;13:198-203 pubmed publisher
    ..We describe a revised model for P(i) signal transduction of the E. coli Pho regulon. ..
  2. Venkatachalam P, Jain A, Sahi S, Raghothama K. Molecular cloning and characterization of phosphate (Pi) responsive genes in Gulf ryegrass (Lolium multiflorum L.): a Pi hyperaccumulator. Plant Mol Biol. 2009;69:1-21 pubmed publisher
    ..Together, these results suggest that the Gulf ryegrass has evolved mechanisms to acquire and hyperaccumulate more Pi under different Pi regimes by activating multiple Pi acquisition and mobilization mechanisms. ..
  3. Jain A, Poling M, Smith A, Nagarajan V, Lahner B, Meagher R, et al. Variations in the composition of gelling agents affect morphophysiological and molecular responses to deficiencies of phosphate and other nutrients. Plant Physiol. 2009;150:1033-49 pubmed publisher
    ..As an alternative, we demonstrate the efficacy of a sterile and contamination-free hydroponic system for dissecting morphophysiological and molecular responses of Arabidopsis to different nutrient deficiencies. ..
  4. Hem S, HogenEsch H. Relationship between physical and chemical properties of aluminum-containing adjuvants and immunopotentiation. Expert Rev Vaccines. 2007;6:685-98 pubmed
  5. Sokolovska A, Hem S, HogenEsch H. Activation of dendritic cells and induction of CD4(+) T cell differentiation by aluminum-containing adjuvants. Vaccine. 2007;25:4575-85 pubmed
    ..These results indicate that aluminum-containing adjuvants activate DCs and influence their ability to direct T(H)1 and T(H)2 responses through the secretion of IL-1beta and IL-18. ..
  6. Morefield G, Sokolovska A, Jiang D, HogenEsch H, Robinson J, Hem S. Role of aluminum-containing adjuvants in antigen internalization by dendritic cells in vitro. Vaccine. 2005;23:1588-95 pubmed
  7. Karthikeyan A, Varadarajan D, Mukatira U, D Urzo M, Damsz B, Raghothama K. Regulated expression of Arabidopsis phosphate transporters. Plant Physiol. 2002;130:221-33 pubmed
    ..The results suggest that members of the Pi transporter family may have similar but nonredundant functions in plants. ..
  8. Chang M, Shi Y, Nail S, HogenEsch H, Adams S, White J, et al. Degree of antigen adsorption in the vaccine or interstitial fluid and its effect on the antibody response in rabbits. Vaccine. 2001;19:2884-9 pubmed
    ..Thus, the degree of adsorption of lysozyme in sheep interstitial fluid rather than the degree of adsorption in the vaccine correlated with the immune response. ..
  9. Liu C, Muchhal U, Raghothama K. Differential expression of TPS11, a phosphate starvation-induced gene in tomato. Plant Mol Biol. 1997;33:867-74 pubmed
    ..The amount of TPSI1 transcript decreased rapidly when Pi-starved tomato plants were resupplied with Pi. These results suggest that TPSI1 gene expression may be a part of the early Pi starvation response mechanism in plants. ..

More Information


  1. Noe S, Green M, HogenEsch H, Hem S. Mechanism of immunopotentiation by aluminum-containing adjuvants elucidated by the relationship between antigen retention at the inoculation site and the immune response. Vaccine. 2010;28:3588-94 pubmed publisher
    ..The presence of an aluminum-containing adjuvant was essential for immunopotentiation, but retention of the antigen at the inoculation site was not required...
  2. Baxter I, Vitek O, Lahner B, Muthukumar B, Borghi M, Morrissey J, et al. The leaf ionome as a multivariable system to detect a plant's physiological status. Proc Natl Acad Sci U S A. 2008;105:12081-6 pubmed publisher
  3. Metcalf W, Steed P, Wanner B. Identification of phosphate starvation-inducible genes in Escherichia coli K-12 by DNA sequence analysis of psi::lacZ(Mu d1) transcriptional fusions. J Bacteriol. 1990;172:3191-200 pubmed
    ..psiE and psiF::lacZ(Mu d1)s lie in uncharacterized open reading frames near the xylE and phoA genes, respectively. Six other psi::lacZ(Mu d1)s lie in yet unreported Escherichia coli sequences. ..
  4. Varadarajan D, Karthikeyan A, Matilda P, Raghothama K. Phosphite, an analog of phosphate, suppresses the coordinated expression of genes under phosphate starvation. Plant Physiol. 2002;129:1232-40 pubmed
    ..Synchronized suppression of multiple Pi starvation-induced genes by Phi points to its action on the early molecular events, probably signal transduction, in Pi starvation response...
  5. Steed P, Wanner B. Use of the rep technique for allele replacement to construct mutants with deletions of the pstSCAB-phoU operon: evidence of a new role for the PhoU protein in the phosphate regulon. J Bacteriol. 1993;175:6797-809 pubmed
    ..Further, our results contradict those of M. Muda, N. N. Rao, and A. Torriani (J. Bacteriol. 174:8057-8064, 1992), who reported that the PhoU protein was required for Pi uptake. ..
  6. Zhang M, Zhou M, Van Etten R, Stauffacher C. Crystal structure of bovine low molecular weight phosphotyrosyl phosphatase complexed with the transition state analog vanadate. Biochemistry. 1997;36:15-23 pubmed
    ..The structure of the vanadate complex may be expected to closely resemble the transition state for reactions catalyzed by protein tyrosine phosphatases. ..
  7. Gumaelius L, Lahner B, Salt D, Banks J. Arsenic hyperaccumulation in gametophytes of Pteris vittata. A new model system for analysis of arsenic hyperaccumulation. Plant Physiol. 2004;136:3198-208 pubmed
    ..vittata using gametophytes as an easily manipulated model system...
  8. Metcalf W, Wanner B. Involvement of the Escherichia coli phn (psiD) gene cluster in assimilation of phosphorus in the form of phosphonates, phosphite, Pi esters, and Pi. J Bacteriol. 1991;173:587-600 pubmed
    ..Furthermore, our discovery of Pi-regulated genes for the assimilation of reduced P suggests that a P redox cycle may be important in biology. ..
  9. Wanner B, Chang B. The phoBR operon in Escherichia coli K-12. J Bacteriol. 1987;169:5569-74 pubmed
    ..Unexpectedly, an antisense pho omega Mu d1(lacZ) insertion within the promoter-proximal end of the phoB gene expressed the lacZ reporter gene, thus allowing for the possibility that the phoBR operon is regulated by an antisense RNA. ..
  10. Miura K, Rus A, Sharkhuu A, Yokoi S, Karthikeyan A, Raghothama K, et al. The Arabidopsis SUMO E3 ligase SIZ1 controls phosphate deficiency responses. Proc Natl Acad Sci U S A. 2005;102:7760-5 pubmed
    ..These results indicate that AtSIZ1 is a SUMO E3 ligase and that sumoylation is a control mechanism that acts both negatively and positively on different Pi deficiency responses. ..
  11. Ramaiah M, Jain A, Baldwin J, Karthikeyan A, Raghothama K. Characterization of the phosphate starvation-induced glycerol-3-phosphate permease gene family in Arabidopsis. Plant Physiol. 2011;157:279-91 pubmed publisher
    ..A 9-fold induction of AtG3Pp3 in the mutant provided some evidence for a lack of functional redundancy in the gene family. These results thus reflect differential roles of members of the G3Pp family in the maintenance of Pi homeostasis. ..
  12. Devaiah B, Madhuvanthi R, Karthikeyan A, Raghothama K. Phosphate starvation responses and gibberellic acid biosynthesis are regulated by the MYB62 transcription factor in Arabidopsis. Mol Plant. 2009;2:43-58 pubmed publisher
    ..Such cross-talk between Pi homeostasis and GA might have broader implications on flowering, root development and adaptive mechanisms during nutrient stress. ..
  13. Wanner B. Gene regulation by phosphate in enteric bacteria. J Cell Biochem. 1993;51:47-54 pubmed
    ..Cross regulation of the PHO regulon by CreC and acetyl phosphate may be examples of underlying control mechanisms important for the general (global) control of cell growth and metabolism. ..
  14. Cao A, Jain A, Baldwin J, Raghothama K. Phosphate differentially regulates 14-3-3 family members and GRF9 plays a role in Pi-starvation induced responses. Planta. 2007;226:1219-30 pubmed
  15. Devaiah B, Nagarajan V, Raghothama K. Phosphate homeostasis and root development in Arabidopsis are synchronized by the zinc finger transcription factor ZAT6. Plant Physiol. 2007;145:147-59 pubmed
    ..To our knowledge, ZAT6 is the first cysteine-2/histidine-2 zinc finger transcription factor reported to regulate root development and nutrient stress responses. ..
  16. Shi Y, HogenEsch H, Regnier F, Hem S. Detoxification of endotoxin by aluminum hydroxide adjuvant. Vaccine. 2001;19:1747-52 pubmed
    ..Aluminum hydroxide adjuvant detoxifies endotoxin by adsorbing it in the vaccine and then not releasing it in interstitial fluid upon administration...
  17. Baldwin J, Karthikeyan A, Raghothama K. LEPS2, a phosphorus starvation-induced novel acid phosphatase from tomato. Plant Physiol. 2001;125:728-37 pubmed
    ..The bacterially (Escherichia coli) expressed protein exhibits phosphatase activity against the synthetic substrate p-nitrophenyl phosphate. The pH optimum of the enzyme activity suggests that LePS2 is an acid phosphatase. ..
  18. Haldimann A, Daniels L, Wanner B. Use of new methods for construction of tightly regulated arabinose and rhamnose promoter fusions in studies of the Escherichia coli phosphate regulon. J Bacteriol. 1998;180:1277-86 pubmed
    ..The growth defect is apparently a consequence of high-level Pst synthesis resulting from autogenous control of PhoB and PhoR synthesis in the absence of PhoU. ..
  19. Wanner B. Genes for phosphonate biodegradation in Escherichia coli. SAAS Bull Biochem Biotechnol. 1992;5:1-6 pubmed
    ..These interpretations are based on results from a combination of molecular genetic and molecular biological studies on phosphonate metabolism in E. coli. ..
  20. Saddoris K, Fleet J, Radcliffe J. Sodium-dependent phosphate uptake in the jejunum is post-transcriptionally regulated in pigs fed a low-phosphorus diet and is independent of dietary calcium concentration. J Nutr. 2010;140:731-6 pubmed publisher
    ..These data suggest that restricting dietary aP concentration by only 43% stimulates Na(+)-dependent phosphate uptake and expression of the NaPi-IIb protein in the BBM of the small intestine and through a post-transcriptional mechanism. ..
  21. Wanner B, Wilmes Riesenberg M. Involvement of phosphotransacetylase, acetate kinase, and acetyl phosphate synthesis in control of the phosphate regulon in Escherichia coli. J Bacteriol. 1992;174:2124-30 pubmed
    ..Further, it provides a functional basis for the concept of cross-regulation in the PHO regulon. This is also the first evidence that acetyl phosphate may have a role as an effector of gene regulation. ..
  22. Devaiah B, Karthikeyan A, Raghothama K. WRKY75 transcription factor is a modulator of phosphate acquisition and root development in Arabidopsis. Plant Physiol. 2007;143:1789-801 pubmed
    ..WRKY75 is the first member of the WRKY transcription factor family reported to be involved in regulating a nutrient starvation response and root development. ..
  23. Liu C, Muchhal U, Uthappa M, Kononowicz A, Raghothama K. Tomato phosphate transporter genes are differentially regulated in plant tissues by phosphorus. Plant Physiol. 1998;116:91-9 pubmed
    ..Divided root-system studies support the hypothesis that signal(s) for the Pi-starvation response may arise internally because of the changes in cellular concentration of phosphorus. ..
  24. Jiang W, Metcalf W, Lee K, Wanner B. Molecular cloning, mapping, and regulation of Pho regulon genes for phosphonate breakdown by the phosphonatase pathway of Salmonella typhimurium LT2. J Bacteriol. 1995;177:6411-21 pubmed
    ..Complementation studies indicate that the S. typhimurium phn locus probably includes genes both for phosphonate transport and for catalysis of C-P bond cleavage. ..
  25. Baldwin J, Karthikeyan A, Cao A, Raghothama K. Biochemical and molecular analysis of LePS2;1: a phosphate starvation induced protein phosphatase gene from tomato. Planta. 2008;228:273-80 pubmed publisher
    ..To our knowledge this is the first comprehensive analysis of a protein phosphatase induced during phosphate starvation...
  26. Karthikeyan A, Ballachanda D, Raghothama K. Promoter deletion analysis elucidates the role of cis elements and 5'UTR intron in spatiotemporal regulation of AtPht1;4 expression in Arabidopsis. Physiol Plant. 2009;136:10-8 pubmed publisher
    ..However, expression of AtPht1;4 in aerial parts of the plant was not influenced by the intron. Together these results suggest that expression of AtPht1;4 in the roots and aerial parts is regulated by independent mechanisms. ..
  27. Iyer S, HogenEsch H, Hem S. Relationship between the degree of antigen adsorption to aluminum hydroxide adjuvant in interstitial fluid and antibody production. Vaccine. 2003;21:1219-23 pubmed
    ..The results indicated that antigen presenting cells can take up desorbed antigen from interstitial fluid as well as antigen adsorbed to aluminum-containing adjuvants...
  28. Shi Y, HogenEsch H, Hem S. Change in the degree of adsorption of proteins by aluminum-containing adjuvants following exposure to interstitial fluid: freshly prepared and aged model vaccines. Vaccine. 2001;20:80-5 pubmed
    ..However, only 60% of the ovalbumin adsorbed to aluminum hydroxide adjuvant was eluted during exposure to sheep lymph fluid for 24h after the model vaccine aged for 11 weeks at 4 degrees C. ..