Experts and Doctors on membrane transport proteins in West Lafayette, Indiana, United States


Locale: West Lafayette, Indiana, United States
Topic: membrane transport proteins

Top Publications

  1. Tan Y, Arnold R, Luo Z. Legionella pneumophila regulates the small GTPase Rab1 activity by reversible phosphorylcholination. Proc Natl Acad Sci U S A. 2011;108:21212-7 pubmed publisher
    ..Our results reveal reversible phosphorylcholination as a mechanism for balanced modulation of host cellular processes by a bacterial pathogen. ..
  2. Spector J, Zakharov S, Lill Y, Sharma O, Cramer W, Ritchie K. Mobility of BtuB and OmpF in the Escherichia coli outer membrane: implications for dynamic formation of a translocon complex. Biophys J. 2010;99:3880-6 pubmed publisher
    ..Thus, formation of the outer membrane translocon for cellular import of the nuclease colicins is a demonstrably dynamic process, because it depends on lateral diffusion of BtuB and collisional interaction with relatively immobile OmpF. ..
  3. Liu Y, Gao P, Banga S, Luo Z. An in vivo gene deletion system for determining temporal requirement of bacterial virulence factors. Proc Natl Acad Sci U S A. 2008;105:9385-90 pubmed publisher
    ..Our study has provided a technical concept for analyzing the temporal requirement of specific bacterial proteins or protein complexes in infection or development. ..
  4. Sharma O, Cramer W. Minimum length requirement of the flexible N-terminal translocation subdomain of colicin E3. J Bacteriol. 2007;189:363-8 pubmed
  5. Heatwole V, Somerville R. Cloning, nucleotide sequence, and characterization of mtr, the structural gene for a tryptophan-specific permease of Escherichia coli K-12. J Bacteriol. 1991;173:108-15 pubmed
    ..The hydropathy plots of the two permeases were similar. Possible operator sites for the tyrosine and tryptophan repressors are situated within the region of DNA that is likely to be the mtr promoter. ..
  6. Yang H, Richter G, Wang X, Młodzińska E, Carraro N, Ma G, et al. Sterols and sphingolipids differentially function in trafficking of the Arabidopsis ABCB19 auxin transporter. Plant J. 2013;74:37-47 pubmed publisher
    ..The accessibility of this system to direct live imaging and biochemical analysis makes it ideal for the modeling and analysis of sterol and sphingolipid regulation of ABCB/P-glycoprotein transporters...
  7. Goole J, Lindley D, Roth W, Carl S, Amighi K, Kauffmann J, et al. The effects of excipients on transporter mediated absorption. Int J Pharm. 2010;393:17-31 pubmed publisher
  8. Sharma O, Yamashita E, Zhalnina M, Zakharov S, Datsenko K, Wanner B, et al. Structure of the complex of the colicin E2 R-domain and its BtuB receptor. The outer membrane colicin translocon. J Biol Chem. 2007;282:23163-70 pubmed
    ..Arg(452) at the R/C-domain interface in colicin E2 was found have an essential role at a putative site of protease cleavage, which would liberate the C-terminal activity domain for passage through the outer membrane translocon. ..
  9. Blakeslee J, Bandyopadhyay A, Lee O, Mravec J, Titapiwatanakun B, Sauer M, et al. Interactions among PIN-FORMED and P-glycoprotein auxin transporters in Arabidopsis. Plant Cell. 2007;19:131-47 pubmed
    ..These results suggest that PINs and PGPs characterize coordinated, independent auxin transport mechanisms but also function interactively in a tissue-specific manner...

More Information


  1. Zakharov S, Zhalnina M, Sharma O, Cramer W. The colicin E3 outer membrane translocon: immunity protein release allows interaction of the cytotoxic domain with OmpF porin. Biochemistry. 2006;45:10199-207 pubmed
  2. Zaitseva J, Zhang H, Binnie R, Hermodson M. The proteins encoded by the rbs operon of Escherichia coli: II. Use of chimeric protein constructs to isolate and characterize RbsC. Protein Sci. 1996;5:1100-7 pubmed
    ..This is a substantial increase in amounts from any previous RbsC production vectors. All proteins from the rbs operon have now been overproduced and substantially purified...
  3. Zakharov S, Eroukova V, Rokitskaya T, Zhalnina M, Sharma O, Loll P, et al. Colicin occlusion of OmpF and TolC channels: outer membrane translocons for colicin import. Biophys J. 2004;87:3901-11 pubmed
    ..These data are considered in the context of a two-receptor translocon model for colicin entry into cells. ..
  4. Leiman P, Basler M, Ramagopal U, Bonanno J, Sauder J, Pukatzki S, et al. Type VI secretion apparatus and phage tail-associated protein complexes share a common evolutionary origin. Proc Natl Acad Sci U S A. 2009;106:4154-9 pubmed publisher
    ..We propose that T6SS is a multicomponent structure whose extracellular part resembles both structurally and functionally a bacteriophage tail, an efficient machine that translocates proteins and DNA across lipid membranes into cells...
  5. Hou Y, Zhu Y, Wang P, Zhao Y, Xie S, Batelli G, et al. Type One Protein Phosphatase 1 and Its Regulatory Protein Inhibitor 2 Negatively Regulate ABA Signaling. PLoS Genet. 2016;12:e1005835 pubmed publisher
    ..Taken together, our results uncover TOPP1 and AtI-2 as negative regulators of ABA signaling. ..
  6. Heatwole V, Somerville R. The tryptophan-specific permease gene, mtr, is differentially regulated by the tryptophan and tyrosine repressors in Escherichia coli K-12. J Bacteriol. 1991;173:3601-4 pubmed
    ..One transcript was induced by the Tyr repressor. The tryptophan-dependent interaction of Trp repressor with an operator target within the mtr promoter was demonstrated by means of a restriction endonuclease protection assay. ..
  7. Barroga C, Zhang H, Wajih N, Bouyer J, Hermodson M. The proteins encoded by the rbs operon of Escherichia coli: I. Overproduction, purification, characterization, and functional analysis of RbsA. Protein Sci. 1996;5:1093-9 pubmed
    ..However, an inconsistency between the several results from deletion strains raises questions regarding the interpretations of the in vivo data...
  8. Carl S, Lindley D, Das D, Couraud P, Weksler B, Romero I, et al. ABC and SLC transporter expression and proton oligopeptide transporter (POT) mediated permeation across the human blood--brain barrier cell line, hCMEC/D3 [corrected]. Mol Pharm. 2010;7:1057-68 pubmed publisher
    ..Additional studies are required to adequately determine the potential to establish an in vivo correlation...
  9. Yang H, Murphy A. Functional expression and characterization of Arabidopsis ABCB, AUX 1 and PIN auxin transporters in Schizosaccharomyces pombe. Plant J. 2009;59:179-91 pubmed publisher
    ..Taken together, these results suggest that the S. pombe system described here can be employed for comparative analyses and subsequent structural characterizations of plant transport proteins. ..
  10. Zakharov S, Sharma O, Zhalnina M, Cramer W. Primary events in the colicin translocon: FRET analysis of colicin unfolding initiated by binding to BtuB and OmpF. Biochemistry. 2008;47:12802-9 pubmed publisher
    ..The R-domain Arg399 is responsible for electrostatic interaction with BtuB. It is concluded that free energy for colicin unfolding is provided by binding of the R- domain to BtuB and binding/insertion of the T-domain to/into OmpF. ..
  11. Titapiwatanakun B, Blakeslee J, Bandyopadhyay A, Yang H, Mravec J, Sauer M, et al. ABCB19/PGP19 stabilises PIN1 in membrane microdomains in Arabidopsis. Plant J. 2009;57:27-44 pubmed publisher
    ..These data suggest that ABCB19 stabilises PIN1 localisation at the plasma membrane in discrete cellular subdomains where PIN1 and ABCB19 expression overlaps...
  12. Haldimann A, Daniels L, Wanner B. Use of new methods for construction of tightly regulated arabinose and rhamnose promoter fusions in studies of the Escherichia coli phosphate regulon. J Bacteriol. 1998;180:1277-86 pubmed
    ..The growth defect is apparently a consequence of high-level Pst synthesis resulting from autogenous control of PhoB and PhoR synthesis in the absence of PhoU. ..
  13. Mukhopadhyay A, Avramova L, Weiner H. Tom34 unlike Tom20 does not interact with the leader sequences of mitochondrial precursor proteins. Arch Biochem Biophys. 2002;400:97-104 pubmed
    ..It is concluded from our observation that Tom34 is a cytosolic protein, whose role appeared to be to interact with mature portion of some preproteins and may keep them in an unfolded, import compatible state. ..
  14. Kurisu G, Zakharov S, Zhalnina M, Bano S, Eroukova V, Rokitskaya T, et al. The structure of BtuB with bound colicin E3 R-domain implies a translocon. Nat Struct Biol. 2003;10:948-54 pubmed
    ..The translocator was concluded to be OmpF from the occlusion of OmpF channels by colicin E3. ..
  15. Oldham M, Davidson A, Chen J. Structural insights into ABC transporter mechanism. Curr Opin Struct Biol. 2008;18:726-33 pubmed publisher
    ..It appears that rigid-body rotations of the transmembrane subunits, coinciding with the opening and closing of the nucleotide-binding subunits, couples ATP hydrolysis to substrate translocation...
  16. Venkatachalam P, Thulaseedharan A, Raghothama K. Molecular identification and characterization of a gene associated with the onset of tapping panel dryness (TPD) syndrome in rubber tree (Hevea brasiliensis Muell.) by mRNA differential display. Mol Biotechnol. 2009;41:42-52 pubmed publisher
    ..These results suggest that down-regulation of HbTOM20 in TPD-affected trees may play an important role in alteration of mitochondrial metabolism resulting in impaired latex biosynthesis...
  17. Zhu W, Banga S, Tan Y, Zheng C, Stephenson R, Gately J, et al. Comprehensive identification of protein substrates of the Dot/Icm type IV transporter of Legionella pneumophila. PLoS ONE. 2011;6:e17638 pubmed publisher
    ..Thus, our efforts have identified a large number of novel substrates of the Dot/Icm system and have revealed the diverse features recognizable by this protein transporter...
  18. Ramaiah M, Jain A, Baldwin J, Karthikeyan A, Raghothama K. Characterization of the phosphate starvation-induced glycerol-3-phosphate permease gene family in Arabidopsis. Plant Physiol. 2011;157:279-91 pubmed publisher
    ..A 9-fold induction of AtG3Pp3 in the mutant provided some evidence for a lack of functional redundancy in the gene family. These results thus reflect differential roles of members of the G3Pp family in the maintenance of Pi homeostasis. ..
  19. Steed P, Wanner B. Use of the rep technique for allele replacement to construct mutants with deletions of the pstSCAB-phoU operon: evidence of a new role for the PhoU protein in the phosphate regulon. J Bacteriol. 1993;175:6797-809 pubmed
    ..Further, our results contradict those of M. Muda, N. N. Rao, and A. Torriani (J. Bacteriol. 174:8057-8064, 1992), who reported that the PhoU protein was required for Pi uptake. ..
  20. Blakeslee J, Bandyopadhyay A, Peer W, Makam S, Murphy A. Relocalization of the PIN1 auxin efflux facilitator plays a role in phototropic responses. Plant Physiol. 2004;134:28-31 pubmed