Experts and Doctors on two hybrid system techniques in Chicago, Illinois, United States


Locale: Chicago, Illinois, United States
Topic: two hybrid system techniques

Top Publications

  1. Draper E, Dubrovskyi O, Bar E, Stone D. Dse1 may control cross talk between the pheromone and filamentation pathways in yeast. Curr Genet. 2009;55:611-21 pubmed publisher
    ..Thus, the interaction of Dse1 with both Gbeta and Ste11 may be designed to control cross talk between the pheromone and filamentation pathways. ..
  2. Viswanathan V, Lukic S, Koutsouris A, Miao R, Muza M, Hecht G. Cytokeratin 18 interacts with the enteropathogenic Escherichia coli secreted protein F (EspF) and is redistributed after infection. Cell Microbiol. 2004;6:987-97 pubmed
    ..These results suggest that modifications in 14-3-3 interactions and IF network, modulated by type III secreted proteins, may be an important step in EPEC pathogenesis. ..
  3. Chen K, Merino C, Sigrist S, Featherstone D. The 4.1 protein coracle mediates subunit-selective anchoring of Drosophila glutamate receptors to the postsynaptic actin cytoskeleton. J Neurosci. 2005;25:6667-75 pubmed
  4. Dell E, Connor J, Chen S, Stebbins E, Skiba N, Mochly Rosen D, et al. The betagamma subunit of heterotrimeric G proteins interacts with RACK1 and two other WD repeat proteins. J Biol Chem. 2002;277:49888-95 pubmed
  5. Niu J, Scheschonka A, Druey K, Davis A, Reed E, Kolenko V, et al. RGS3 interacts with 14-3-3 via the N-terminal region distinct from the RGS (regulator of G-protein signalling) domain. Biochem J. 2002;365:677-84 pubmed
    ..This study describes a new level in the regulation of G-protein signalling, in which the inhibitors of G-proteins, RGS proteins, can themselves be regulated by phosphorylation and binding 14-3-3. ..
  6. Oh Y, Fung L. Brain proteins interacting with the tetramerization region of non-erythroid alpha spectrin. Cell Mol Biol Lett. 2007;12:604-20 pubmed
    ..Future structural and functional studies of these proteins to provide interaction mechanisms will no doubt lead to a better understanding of brain physiology and pathophysiology. ..
  7. Li X, Chen H, Oh S, Chishti A. A Presenilin-like protease associated with Plasmodium falciparum micronemes is involved in erythrocyte invasion. Mol Biochem Parasitol. 2008;158:22-31 pubmed
    ..PfSPP is the first microneme-associated intramembrane aspartyl protease identified in the apicomplexan parasites that interacts with a major transmembrane receptor on host erythrocytes. ..
  8. Qi C, Chang J, Zhu Y, Yeldandi A, Rao S, Zhu Y. Identification of protein arginine methyltransferase 2 as a coactivator for estrogen receptor alpha. J Biol Chem. 2002;277:28624-30 pubmed
    ..In this respect PRMT2 differs from coactivators PRMT1 and CARM1 (coactivator-associated arginine methyltransferase). These results suggest that PRMT2 is a novel ERalpha coactivator. ..
  9. Stohl E, Brockman J, Burkle K, Morimatsu K, Kowalczykowski S, Seifert H. Escherichia coli RecX inhibits RecA recombinase and coprotease activities in vitro and in vivo. J Biol Chem. 2003;278:2278-85 pubmed
    ..Our combined in vitro and in vivo data indicate that RecX can inhibit both RecA recombinase and coprotease activities. ..

More Information


  1. Peterson R, Andhare R, Rousche K, Knudson W, Wang W, Grossfield J, et al. CD44 modulates Smad1 activation in the BMP-7 signaling pathway. J Cell Biol. 2004;166:1081-91 pubmed
    ..These results support a functional link between the BMP signaling cascade and CD44. Thus, changes in hyaluronan-cell interactions may serve as a means to modulate cellular responsiveness to BMP. ..
  2. Brooke J, Cha J, Eidels L. Latent transforming growth factor beta-binding protein-3 and fibulin-1C interact with the extracellular domain of the heparin-binding EGF-like growth factor precursor. BMC Cell Biol. 2002;3:2 pubmed
    ..The interactions of the extracellular domain of proHB-EGF with LTBP-3 and fibulin-1C suggest novel functions for HB-EGF between cell and tissue surfaces. ..
  3. Jiang F, Ai J, Xiao W, Wang Z. FB1, an E2A fusion partner in childhood leukemia, interacts with U19/EAF2 and inhibits its transcriptional activity. Cancer Lett. 2007;253:265-72 pubmed
    ..Interestingly, FB1 inhibited the transcriptional activity of U19/EAF2 but not EAF1. FB1 is an important binding partner and a functional regulator of U19/EAF2, EAF1, and/or ELL. ..
  4. Marty C, Browning D, Ye R. Identification of tetratricopeptide repeat 1 as an adaptor protein that interacts with heterotrimeric G proteins and the small GTPase Ras. Mol Cell Biol. 2003;23:3847-58 pubmed
    ..Thus, TPR1 is a novel adaptor protein for Ras and selected Galpha proteins that may be involved in protein-protein interaction relating to G-protein signaling. ..
  5. Erfurth F, Hemenway C, de Erkenez A, Domer P. MLL fusion partners AF4 and AF9 interact at subnuclear foci. Leukemia. 2004;18:92-102 pubmed
    ..The disruption of this web of interactions by fusion with MLL may be important to leukemogenesis. ..
  6. Wolfe B, Takaki T, Petronczki M, Glotzer M. Polo-like kinase 1 directs assembly of the HsCyk-4 RhoGAP/Ect2 RhoGEF complex to initiate cleavage furrow formation. PLoS Biol. 2009;7:e1000110 pubmed publisher
    ..Collectively, our data reveal that formation of the HsCyk-4-Ect2 complex is subject to multiple layers of regulation to ensure that RhoA activation occurs between the segregated sister chromatids during anaphase. ..
  7. Samanta J, Kessler J. Interactions between ID and OLIG proteins mediate the inhibitory effects of BMP4 on oligodendroglial differentiation. Development. 2004;131:4131-42 pubmed
  8. Cvetanovic M, Rooney R, Garcia J, Toporovskaya N, Zoghbi H, Opal P. The role of LANP and ataxin 1 in E4F-mediated transcriptional repression. EMBO Rep. 2007;8:671-7 pubmed
    ..These results provide the first functional link, to our knowledge, between LANP and ataxin 1, and indicate a potential mechanism for the transcriptional aberrations observed in SCA1. ..
  9. Xu Y, Leung C, Lee D, Kennedy B, Crispino J. MTB, the murine homolog of condensin II subunit CAP-G2, represses transcription and promotes erythroid cell differentiation. Leukemia. 2006;20:1261-9 pubmed
  10. Sengupta S, Miller K, Homma K, Edge R, Cheatham M, Dallos P, et al. Interaction between the motor protein prestin and the transporter protein VAPA. Biochim Biophys Acta. 2010;1803:796-804 pubmed publisher
    ..These data suggest that VAPA could be involved in prestin's transportation inside OHCs and may facilitate the targeting of this abundant OHC protein to the plasma membrane. ..
  11. Herzig R, Andersson U, Scarpulla R. Dynein light chain interacts with NRF-1 and EWG, structurally and functionally related transcription factors from humans and drosophila. J Cell Sci. 2000;113 Pt 23:4263-73 pubmed
    ..Thus, the interaction with dynein light chain is conserved between transcription factors that are structurally and functionally similar between humans and Drosophila. ..
  12. Niu J, Vaiskunaite R, Suzuki N, Kozasa T, Carr D, Dulin N, et al. Interaction of heterotrimeric G13 protein with an A-kinase-anchoring protein 110 (AKAP110) mediates cAMP-independent PKA activation. Curr Biol. 2001;11:1686-90 pubmed
    ..Finally, AKAP110 significantly potentiated G alpha 13-induced activation of PKA. Thus, AKAP110 provides a link between heterotrimeric G proteins and cAMP-independent activation of PKA. ..
  13. Grasberger H, Bell G. Subcellular recruitment by TSG118 and TSPYL implicates a role for zinc finger protein 106 in a novel developmental pathway. Int J Biochem Cell Biol. 2005;37:1421-37 pubmed
    ..These results identify ZFP106 as a potential player in a novel pathway involved in testis development. ..
  14. Wentz Hunter K, Ueda J, Yue B. Protein interactions with myocilin. Invest Ophthalmol Vis Sci. 2002;43:176-82 pubmed
    ..The interaction of myocilin with RLC, a component of the myosin motor protein complex, implies a role for myocilin in the actomyosin system, linking in turn this novel protein to functional status of the TM. ..
  15. Chen X, Bonne S, Hatzfeld M, van Roy F, Green K. Protein binding and functional characterization of plakophilin 2. Evidence for its diverse roles in desmosomes and beta -catenin signaling. J Biol Chem. 2002;277:10512-22 pubmed
  16. Wu J, Luo S, Jiang H, Li H. Mammalian CHORD-containing protein 1 is a novel heat shock protein 90-interacting protein. FEBS Lett. 2005;579:421-6 pubmed
    ..The unique Chp-1-Hsp90 interaction may be indicative of a distinct biological activity of Chp-1 and functional diversification of CHORD-containing proteins during evolution. ..
  17. Regan J, Laimins L. Bap31 is a novel target of the human papillomavirus E5 protein. J Virol. 2008;82:10042-51 pubmed publisher
    ..These studies identify a novel binding partner of the high-risk HPV E5 proteins and provide insight into how the E5 proteins may modulate the life cycle in differentiating cells...
  18. Andreeva A, Kutuzov M, Vaiskunaite R, Profirovic J, Meigs T, Predescu S, et al. G alpha12 interaction with alphaSNAP induces VE-cadherin localization at endothelial junctions and regulates barrier function. J Biol Chem. 2005;280:30376-83 pubmed
  19. Kroczynska B, King Simmons L, Alloza L, Alava M, Elguindi E, Blond S. BIP co-chaperone MTJ1/ERDJ1 interacts with inter-alpha-trypsin inhibitor heavy chain 4. Biochem Biophys Res Commun. 2005;338:1467-77 pubmed
    ..This work reveals that the SANT2 domain of HTJ1 is a genuine protein-protein interaction module. ..
  20. Lapik Y, Kaufman L. The Arabidopsis cupin domain protein AtPirin1 interacts with the G protein alpha-subunit GPA1 and regulates seed germination and early seedling development. Plant Cell. 2003;15:1578-90 pubmed
    ..These data indicate that AtPirin1 likely functions immediately downstream of GPA1 in regulating seed germination and early seedling development. ..
  21. Okano K, Schnaper H, Bomsztyk K, Hayashida T. RACK1 binds to Smad3 to modulate transforming growth factor-beta1-stimulated alpha2(I) collagen transcription in renal tubular epithelial cells. J Biol Chem. 2006;281:26196-204 pubmed
    ..These results suggest that RACK1 modulates transcription of alpha2(I) collagen by TGF-beta1 through interference with Smad3 binding to the gene promoter. ..
  22. Warpeha K, Upadhyay S, Yeh J, Adamiak J, Hawkins S, Lapik Y, et al. The GCR1, GPA1, PRN1, NF-Y signal chain mediates both blue light and abscisic acid responses in Arabidopsis. Plant Physiol. 2007;143:1590-600 pubmed
    ..We also demonstrate that this mechanism is present in imbibed seeds wherein it affects germination rate. ..
  23. Bu H, Kashireddy P, Chang J, Zhu Y, Zhang Z, Zheng W, et al. ERBP, a novel estrogen receptor binding protein enhancing the activity of estrogen receptor. Biochem Biophys Res Commun. 2004;317:54-9 pubmed
    ..The interaction of ERBP with ERalpha was further confirmed in vivo by immunoprecipitation. Transient transfection experiment demonstrated that ERBP enhanced the transcriptional activity of ERalpha. ..
  24. Smith E, Xu Y, Tomson B, Leung C, Fujiwara Y, Orkin S, et al. More than blood, a novel gene required for mammalian postimplantation development. Mol Cell Biol. 2004;24:1168-73 pubmed
    ..Furthermore, Mtb(-/-) blastocysts exhibited a higher frequency of apoptotic cells than wild-type or heterozygous blastocysts. These findings demonstrate that Mtb is a novel gene that is essential for early embryonic development. ..
  25. Rajan S, Plant L, Rabin M, Butler M, Goldstein S. Sumoylation silences the plasma membrane leak K+ channel K2P1. Cell. 2005;121:37-47 pubmed
    ..Removal of the peptide adduct by SUMO protease reveals K2P1 to be a K+-selective, pH-sensitive, openly rectifying channel regulated by reversible peptide linkage. ..
  26. Krause A, Zacharias W, Camarata T, Linkhart B, Law E, Lischke A, et al. Tbx5 and Tbx4 transcription factors interact with a new chicken PDZ-LIM protein in limb and heart development. Dev Biol. 2004;273:106-20 pubmed
    ..The identification of a new Tbx5/4-specific binding factor further suggests a novel mechanism for Tbx transcription factor regulation in development and disease. ..
  27. Park K, Kim K, Howard A, Stark B, Webster D. Vitreoscilla hemoglobin binds to subunit I of cytochrome bo ubiquinol oxidases. J Biol Chem. 2002;277:33334-7 pubmed
  28. Mislow J, Holaska J, Kim M, Lee K, Segura Totten M, Wilson K, et al. Nesprin-1alpha self-associates and binds directly to emerin and lamin A in vitro. FEBS Lett. 2002;525:135-40 pubmed
    ..We propose that membrane-anchored nesprin-1alpha antiparallel dimers interact with both emerin and lamin A to provide scaffolding at the inner nuclear membrane. ..
  29. Lipatova Z, Tokarev A, Jin Y, Mulholland J, Weisman L, Segev N. Direct interaction between a myosin V motor and the Rab GTPases Ypt31/32 is required for polarized secretion. Mol Biol Cell. 2008;19:4177-87 pubmed publisher
    ..Together, these results indicate that Ypt31/32 play roles in both the formation of trans-Golgi vesicles and their subsequent Myo2-dependent motility. ..
  30. Chen S, Chen S, Tokarev A, Liu F, Jedd G, Segev N. Ypt31/32 GTPases and their novel F-box effector protein Rcy1 regulate protein recycling. Mol Biol Cell. 2005;16:178-92 pubmed
  31. Yue L, Lu S, Garces J, Jin T, Li J. Protein kinase C-regulated dynamitin-macrophage-enriched myristoylated alanine-rice C kinase substrate interaction is involved in macrophage cell spreading. J Biol Chem. 2000;275:23948-56 pubmed
    ..Furthermore, the regulation of this interaction by PKC and Ca(2+)/calmodulin provides a possible regulatory mechanism for cell adhesion and spreading. ..