Experts and Doctors on calcium in Urbana, Illinois, United States


Locale: Urbana, Illinois, United States
Topic: calcium

Top Publications

  1. Scott D, Docampo R. Characterization of isolated acidocalcisomes of Trypanosoma cruzi. J Biol Chem. 2000;275:24215-21 pubmed
  2. Seufferheld M, Kim K, Whitfield J, Valerio A, Caetano Anolles G. Evolution of vacuolar proton pyrophosphatase domains and volutin granules: clues into the early evolutionary origin of the acidocalcisome. Biol Direct. 2011;6:50 pubmed publisher
    ..This result is remarkable and highlights the possibility that a high degree of cellular compartmentalization could already have been present in the LUCA. ..
  3. Prakasam A, Chien Y, Maruthamuthu V, Leckband D. Calcium site mutations in cadherin: impact on adhesion and evidence of cooperativity. Biochemistry. 2006;45:6930-9 pubmed
    ..However, the influence of the calcium sites on cadherin structure and function depends on their location in the protein. ..
  4. Ohkubo Y, Tajkhorshid E. Distinct structural and adhesive roles of Ca2+ in membrane binding of blood coagulation factors. Structure. 2008;16:72-81 pubmed publisher
  5. Kummerow F, Zhou Q, Mahfouz M, Smiricky M, Grieshop C, Schaeffer D. Trans fatty acids in hydrogenated fat inhibited the synthesis of the polyunsaturated fatty acids in the phospholipid of arterial cells. Life Sci. 2004;74:2707-23 pubmed
  6. Gumbart J, Wiener M, Tajkhorshid E. Coupling of calcium and substrate binding through loop alignment in the outer-membrane transporter BtuB. J Mol Biol. 2009;393:1129-42 pubmed publisher
    ..Based on our results, we suggest that the large size of cobalamin compared to other TonB-dependent transporter substrates explains the requirement of Ca(2+) binding for high-affinity substrate recruitment in BtuB. ..
  7. Moreno S, Docampo R. Calcium regulation in protozoan parasites. Curr Opin Microbiol. 2003;6:359-64 pubmed
    ..Recent findings have shed light on the function of these transporters, the roles that they play in cellular metabolism and their potential use for targeting them for new therapies. ..
  8. Boettcher J, Davis Harrison R, Clay M, Nieuwkoop A, Ohkubo Y, Tajkhorshid E, et al. Atomic view of calcium-induced clustering of phosphatidylserine in mixed lipid bilayers. Biochemistry. 2011;50:2264-73 pubmed publisher
    ..The results of MD simulations performed on PS lipid bilayers in the presence or absence of Ca(2+) provide an atomic view of the conformational effects underlying the observed spectra. ..
  9. Marchesini N, Ruiz F, Vieira M, Docampo R. Acidocalcisomes are functionally linked to the contractile vacuole of Dictyostelium discoideum. J Biol Chem. 2002;277:8146-53 pubmed
    ..Together, these results suggest that the mass-dense granules of D. discoideum are homologous to the acidocalcisomes described in protozoan parasites and are linked to the function of the contractile vacuole. ..

More Information


  1. West D, Qin Y, Peterson Q, Thomas D, Palchaudhuri R, Morrison K, et al. Differential effects of procaspase-3 activating compounds in the induction of cancer cell death. Mol Pharm. 2012;9:1425-34 pubmed publisher
    ..These studies suggest strategies for anticancer clinical development, specifically bolus dosing for PAC-1 and continuous rate infusion for S-PAC-1. ..
  2. Docampo R, Moreno S. Acidocalcisome: A novel Ca2+ storage compartment in trypanosomatids and apicomplexan parasites. Parasitol Today. 1999;15:443-8 pubmed
    ..A number of energy-utilizing pumps and exchangers have been found in these organelles, which underlines their importance in the homeostasis of different elements, as discussed here by Roberto Docampo and Silvia Moreno. ..
  3. Oh M, Wu X, Kim H, Harper J, Zielinski R, Clouse S, et al. CDPKs are dual-specificity protein kinases and tyrosine autophosphorylation attenuates kinase activity. FEBS Lett. 2012;586:4070-5 pubmed publisher
    ..While much remains to be done, it is clear that several CDPKs are dual-specificity kinases, which raises the possibility that phosphotyrosine signaling may play a role in Ca(2+)/CDPK-mediated processes. ..
  4. Vetting M, Bouvier J, Gerlt J, Almo S. Purification, crystallization and structural elucidation of D-galactaro-1,4-lactone cycloisomerase from Agrobacterium tumefaciens involved in pectin degradation. Acta Crystallogr F Struct Biol Commun. 2016;72:36-41 pubmed publisher
    ..The lactone ring appears to be oriented within the active site by stacking interactions with Trp298. ..
  5. Sanders J, Song Y, Chan J, Zhang Y, Jennings S, Kosztowski T, et al. Pyridinium-1-yl bisphosphonates are potent inhibitors of farnesyl diphosphate synthase and bone resorption. J Med Chem. 2005;48:2957-63 pubmed
    ..As such, they represent useful new leads for the discovery of new bone resorption, antiinfective and anticancer drugs. ..
  6. Srinivas S, Cronan J. An Eight-Residue Deletion in Escherichia coli FabG Causes Temperature-Sensitive Growth and Lipid Synthesis Plus Resistance to the Calmodulin Inhibitor Trifluoperazine. J Bacteriol. 2017;199: pubmed publisher
    ..Understanding its structural dynamics and its influence on lipid A synthesis also allows us to explore lipid homeostasis as a mechanism for antibiotic resistance. ..
  7. Sasser T, Padolina M, Fratti R. The yeast vacuolar ABC transporter Ybt1p regulates membrane fusion through Ca2+ transport modulation. Biochem J. 2012;448:365-72 pubmed publisher
    ..We also observed a striking delay in SNARE-dependent Ca2+ efflux. As vacuole fusion can be inhibited by high Ca2+ concentrations, we suggest that the delayed efflux in ybt1? vacuoles leads to the enhanced SNARE function...
  8. Luan B, Carr R, Caffrey M, Aksimentiev A. The effect of calcium on the conformation of cobalamin transporter BtuB. Proteins. 2010;78:1153-62 pubmed publisher
    ..Finally, we characterize conformation dynamics of the TonB box in different X-ray structures and find an interesting correlation between the stability of the TonB box structure and calcium binding. ..
  9. Sasser T, Lawrence G, Karunakaran S, Brown C, Fratti R. The yeast ATP-binding cassette (ABC) transporter Ycf1p enhances the recruitment of the soluble SNARE Vam7p to vacuoles for efficient membrane fusion. J Biol Chem. 2013;288:18300-10 pubmed publisher
    ..The attenuated fusion of ycf1? vacuoles was rescued by the addition of recombinant Vam7p to in vitro experiments. Thus, Ycf1p contributes in the recruitment of Vam7p to the vacuole for efficient membrane fusion. ..
  10. Beatty J, Sylwestrak E, Cox C. Two distinct populations of projection neurons in the rat lateral parafascicular thalamic nucleus and their cholinergic responsiveness. Neuroscience. 2009;162:155-73 pubmed publisher
    ..Our results indicate two distinct subpopulations of lPf projection neurons, and interestingly lPf neurons respond differentially to muscarinic receptor activation based on their axonal target. ..
  11. Cam A, Sivaguru M, Gonzalez de Mejia E. Endocytic mechanism of internalization of dietary peptide lunasin into macrophages in inflammatory condition associated with cardiovascular disease. PLoS ONE. 2013;8:e72115 pubmed publisher
    ..Lunasin may be responsible for attenuation of CVD risk factors by interacting with pathways involved in endocytosis and inflammation...
  12. Seufferheld M, Lea C, Vieira M, Oldfield E, Docampo R. The H(+)-pyrophosphatase of Rhodospirillum rubrum is predominantly located in polyphosphate-rich acidocalcisomes. J Biol Chem. 2004;279:51193-202 pubmed
    ..Taken together, these results suggest that the H(+)-PPase of R. rubrum has two distinct roles depending on its location acting as an intracellular proton pump in acidocalcisomes but in PPi synthesis in the chromatophore membranes...
  13. Garden R, Shippy S, Li L, Moroz T, Sweedler J. Proteolytic processing of the Aplysia egg-laying hormone prohormone. Proc Natl Acad Sci U S A. 1998;95:3972-7 pubmed
    ..A differential peptide distribution is observed at a specific target cell, and a low-frequency variation of AP, [Thr21]AP, is detected in a single animal...
  14. Krivoshik A, Barr L. Force relaxes before the fall of cytosolic calcium in the photomechanical response of rat sphincter pupillae. Am J Physiol Cell Physiol. 2000;279:C274-80 pubmed
    ..We conclude that control of contraction in the sphincter pupillae probably involves an inhibitory process as well as activation by [Ca(2+)](i)...
  15. Buaboocha T, Liao B, Zielinski R. Isolation of cDNA and genomic DNA clones encoding a calmodulin-binding protein related to a family of ATPases involved in cell division and vesicle fusion. Planta. 2001;212:774-81 pubmed
    ..DNA blot hybridization analyses indicate that a single-copy gene in Arabidopsis is likely to encode CIP111. ..
  16. Luo S, Rohloff P, Cox J, Uyemura S, Docampo R. Trypanosoma brucei plasma membrane-type Ca(2+)-ATPase 1 (TbPMC1) and 2 (TbPMC2) genes encode functional Ca(2+)-ATPases localized to the acidocalcisomes and plasma membrane, and essential for Ca(2+) homeostasis and growth. J Biol Chem. 2004;279:14427-39 pubmed publisher
    ..These results establish that T. brucei PMCA-Ca(2+)-ATPases are essential for parasite viability and validate them as targets for drug development...
  17. Seufferheld M, Vieira M, Ruiz F, Rodrigues C, Moreno S, Docampo R. Identification of organelles in bacteria similar to acidocalcisomes of unicellular eukaryotes. J Biol Chem. 2003;278:29971-8 pubmed publisher
    ..The results suggest that acidocalcisomes arose before the prokaryotic and eukaryotic lineages diverged...
  18. Lee A, Kirichenko A, Vygodina T, Siletsky S, Das T, Rousseau D, et al. Ca(2+)-binding site in Rhodobacter sphaeroides cytochrome C oxidase. Biochemistry. 2002;41:8886-98 pubmed
    ..It is proposed that the tightly bound Ca(2+) plays a structural role in the bacterial oxidases while the reversible binding with the mammalian enzyme may be involved in the regulation of mitochondrial function...
  19. Oh M, Kim H, Wu X, Clouse S, Zielinski R, Huber S. Calcium/calmodulin inhibition of the Arabidopsis BRASSINOSTEROID-INSENSITIVE 1 receptor kinase provides a possible link between calcium and brassinosteroid signalling. Biochem J. 2012;443:515-23 pubmed publisher
    ..coli. These results establish a novel assay for recombinant BRI1 transphosphorylation activity and collectively uncover a possible new link between Ca2+ and BR signalling. ..
  20. Ruiz F, Lea C, Oldfield E, Docampo R. Human platelet dense granules contain polyphosphate and are similar to acidocalcisomes of bacteria and unicellular eukaryotes. J Biol Chem. 2004;279:44250-7 pubmed
    ..The results suggest that acidocalcisomes have been conserved during evolution from bacteria to humans...
  21. Ouyang H, Han H, Roh J, Hemp J, Hosler J, Gennis R. Functional importance of a pair of conserved glutamic acid residues and of Ca(2+) binding in the cbb(3)-type oxygen reductases from Rhodobacter sphaeroides and Vibrio cholerae. Biochemistry. 2012;51:7290-6 pubmed publisher
    ..A more likely role for these glutamates is the maintenance of the structural integrity of the active conformation of the enzyme...
  22. Hakansson K, Lim N, Hoppe H, Reid K. Crystal structure of the trimeric alpha-helical coiled-coil and the three lectin domains of human lung surfactant protein D. Structure. 1999;7:255-64 pubmed
    ..The positively charged surface might facilitate binding to negatively charged structures, such as lipopolysaccharides. ..
  23. Ohsako S, Janulis L, Hayashi Y, Bunick D. Characterization of domains in mice of calnexin-t, a putative molecular chaperone required in sperm fertility, with use of glutathione S-transferase-fusion proteins. Biol Reprod. 1998;59:1214-23 pubmed
    ..In addition, the cytoplasmic domain of calnexin-t was shown to be highly phosphorylated immediately after protein synthesis and constitutively phosphorylated during spermatogenesis. ..
  24. Nguyen T, Chen K. Role of divalent cations in plasmid DNA adsorption to natural organic matter-coated silica surface. Environ Sci Technol. 2007;41:5370-5 pubmed
    ..In the case of the DNA layer adsorbed in the presence of CaCl2 without background Na+, softening of the layer does not occur with the rinses...
  25. Piedras RenterĂ­a E, Chen C, Best P. Antisense oligonucleotides against rat brain alpha1E DNA and its atrial homologue decrease T-type calcium current in atrial myocytes. Proc Natl Acad Sci U S A. 1997;94:14936-41 pubmed
    ..These results support the hypothesis that a member of the E class of calcium channel genes encodes a low voltage-activated calcium channel in atrial myocytes. ..
  26. Ohsako S, Hayashi Y, Bunick D. Molecular cloning and sequencing of calnexin-t. An abundant male germ cell-specific calcium-binding protein of the endoplasmic reticulum. J Biol Chem. 1994;269:14140-8 pubmed
    ..These results suggest that spermatogenic cell endoplasmic reticulum has a unique calcium binding protein, calnexin-t, which appears to be a calnexin variant. ..
  27. Zielinski R. Characterization of three new members of the Arabidopsis thaliana calmodulin gene family: conserved and highly diverged members of the gene family functionally complement a yeast calmodulin null. Planta. 2002;214:446-55 pubmed
    ..CaM9, and to a lesser degree CaM8, however, appear to represent Ca2+-binding sensor proteins that interact with a more limited set of target proteins than do more conventional CaM isoforms. ..
  28. Yoon T, Lu X, Diao J, Lee S, Ha T, Shin Y. Complexin and Ca2+ stimulate SNARE-mediated membrane fusion. Nat Struct Mol Biol. 2008;15:707-13 pubmed publisher
    ..We suggest that SNARE complexes, complexins and phospholipids collectively form a complex substrate for Ca(2+) and Ca(2+)-sensing fusion effectors in neurotransmitter release. ..
  29. Che T, Adeola O, Azain M, Carter S, Cromwell G, Hill G, et al. Effect of dietary acids on growth performance of nursery pigs: a cooperative study. J Anim Sci. 2012;90:4408-13 pubmed publisher
    ..05). In summary, under the conditions of this experiment, the acid treatments had no effect but the antibiotic improved growth performance...