Experts and Doctors on bacterial genes in Chicago, Illinois, United States


Locale: Chicago, Illinois, United States
Topic: bacterial genes

Top Publications

  1. Silver S, Phung L. Bacterial heavy metal resistance: new surprises. Annu Rev Microbiol. 1996;50:753-89 pubmed
    ..Finally, the first bacterial metallothionein (which by definition is a small protein that binds metal cations by means of numerous cysteine thiolates) has been characterized in cyanobacteria. ..
  2. Green J, Merkel W, Nichols B. Characterization and sequence of Escherichia coli pabC, the gene encoding aminodeoxychorismate lyase, a pyridoxal phosphate-containing enzyme. J Bacteriol. 1992;174:5317-23 pubmed
  3. Ueda S, Shiroza T, Kuramitsu H. Sequence analysis of the gtfC gene from Streptococcus mutans GS-5. Gene. 1988;69:101-9 pubmed
    ..The possible evolution of the gtfC gene in S. mutans GS-5 is also discussed. ..
  4. Green J, Nichols B. p-Aminobenzoate biosynthesis in Escherichia coli. Purification of aminodeoxychorismate lyase and cloning of pabC. J Biol Chem. 1991;266:12971-5 pubmed
    ..coli chromosome and lies on a 7.5-kilobase pair EcoRI fragment. A strain harboring a pACYC184 recombinant containing pabC overproduced aminodeoxychorismate lyase activity 77-fold. ..
  5. Nies D, Nies A, Chu L, Silver S. Expression and nucleotide sequence of a plasmid-determined divalent cation efflux system from Alcaligenes eutrophus. Proc Natl Acad Sci U S A. 1989;86:7351-5 pubmed
    ..9-kilobase fragment, constructed, and expressed in E. coli under the control of the phage T7 promoter and in A. eutrophus under the control of the lac promoter...
  6. Kato J, Chu L, Kitano K, DeVault J, Kimbara K, Chakrabarty A, et al. Nucleotide sequence of a regulatory region controlling alginate synthesis in Pseudomonas aeruginosa: characterization of the algR2 gene. Gene. 1989;84:31-8 pubmed
  7. Connolly D, Winkler M. Genetic and physiological relationships among the miaA gene, 2-methylthio-N6-(delta 2-isopentenyl)-adenosine tRNA modification, and spontaneous mutagenesis in Escherichia coli K-12. J Bacteriol. 1989;171:3233-46 pubmed
    ..In addition, the results are consistent with the idea that mechanisms exist to increase spontaneous mutation frequency when cells need to adapt to environmental stress. ..
  8. Skaar E, Lazio M, Seifert H. Roles of the recJ and recN genes in homologous recombination and DNA repair pathways of Neisseria gonorrhoeae. J Bacteriol. 2002;184:919-27 pubmed
    ..These results describe the role of recJ and recN in the recombination-dependent processes of the gonococcus and further define the pathways of homologous recombination in this organism...
  9. Gupta A, Phung L, Taylor D, Silver S. Diversity of silver resistance genes in IncH incompatibility group plasmids. Microbiology. 2001;147:3393-402 pubmed publisher
    ..The silA homologue knockout was complemented back to wild-type resistance by the same gene cloned on a plasmid. Homologues of sil genes have also been identified on other enterobacterial genomes...

More Information


  1. Zhu X, Rebello J, Matsumura P, Volz K. Crystal structures of CheY mutants Y106W and T87I/Y106W. CheY activation correlates with movement of residue 106. J Biol Chem. 1997;272:5000-6 pubmed
    ..Specifically, CheY's signaling ability correlates with the conformational heterogeneity of the Tyr106 side chain. Our data also suggest that these mutations affect the signal at an event subsequent to phosphorylation...
  2. Shankar S, Schlictman D, Chakrabarty A. Regulation of nucleoside diphosphate kinase and an alternative kinase in Escherichia coli: role of the sspA and rnk genes in nucleoside triphosphate formation. Mol Microbiol. 1995;17:935-43 pubmed
    ..Additionally, a role for the stringent starvation protein (SspA) in the modulation of nucleoside triphosphate (NTP) levels in E. coli is also suggested from these experiments. ..
  3. Schwan W, Seifert H, Duncan J. Analysis of the fimB promoter region involved in type 1 pilus phase variation in Escherichia coli. Mol Gen Genet. 1994;242:623-30 pubmed
    ..This putative binding protein may be involved in the regulation of fimB, perhaps by acting as a transcriptional activator. ..
  4. Ross C, Winkler M. Structure of the Caulobacter crescentus trpFBA operon. J Bacteriol. 1988;170:757-68 pubmed
    ..crescentus and other bacteria. Taken together, these results are relevant to the analysis of gene expression in C. crescentus and the study of trp gene structure and regulation. ..
  5. Kurnasov O, Goral V, Colabroy K, Gerdes S, Anantha S, Osterman A, et al. NAD biosynthesis: identification of the tryptophan to quinolinate pathway in bacteria. Chem Biol. 2003;10:1195-204 pubmed
    ..Representative examples of each of these genes were overexpressed, and the predicted functions are confirmed in each case using unambiguous biochemical assays...
  6. Deretic V, Dikshit R, Konyecsni W, Chakrabarty A, Misra T. The algR gene, which regulates mucoidy in Pseudomonas aeruginosa, belongs to a class of environmentally responsive genes. J Bacteriol. 1989;171:1278-83 pubmed
    ..These findings indicate that novel conditions in lungs affected by cystic fibrosis may be participating in the control of mucoidy. ..
  7. Dong X, Womble D, Rownd R. Transcriptional pausing in a region important for plasmid NR1 replication control. J Bacteriol. 1987;169:5353-63 pubmed
  8. Skaar E, Humayun M, Bae T, Debord K, Schneewind O. Iron-source preference of Staphylococcus aureus infections. Science. 2004;305:1626-8 pubmed
    ..aureus. Heme iron scavenging by means of hts is required for staphylococcal pathogenesis in animal hosts, indicating that heme iron is the preferred iron source during the initiation of infection...
  9. Seifert H, Wilson D. Characterization of a cryptic gene pair from Neisseria gonorrhoeae that is common to pathogenic Neisseria species. Infect Immun. 1992;60:1232-6 pubmed
    ..Homologs of the two genes were detected in pathogenic Neisseria species but not in commensal species. These genes are designated cnp1 and cnp2 (cryptic neisserial protein). ..
  10. Montgomery C, Boyle Vavra S, Daum R. The arginine catabolic mobile element is not associated with enhanced virulence in experimental invasive disease caused by the community-associated methicillin-resistant Staphylococcus aureus USA300 genetic background. Infect Immun. 2009;77:2650-6 pubmed publisher
    ..Nor was the presence of ACME associated with increased dermonecrosis in a model of skin infection. We conclude that ACME is not necessary for virulence in rodent models of CA-MRSA USA300 pneumonia or skin infection. ..
  11. David M, Rudolph K, Hennessy T, Boyle Vavra S, Daum R. Molecular epidemiology of methicillin-resistant Staphylococcus aureus, rural southwestern Alaska. Emerg Infect Dis. 2008;14:1693-9 pubmed publisher
    ..1% were ST8. Among 120 prospective isolates, 57.5% were clindamycin resistant. CA-MRSA epidemiology in southwestern Alaska differs from that in the lower 48 states; ST8 strains were rarely identified and clindamycin resistance was common...
  12. Markovitz A. A new in vivo termination function for DNA polymerase I of Escherichia coli K12. Mol Microbiol. 2005;55:1867-82 pubmed
    ..To explain these results we propose a new in vivo function for wild-type DNA polymerase I in chromosome termination at site(s) not yet identified. ..
  13. Zago A, Chugani S, Chakrabarty A. Cloning and characterization of polyphosphate kinase and exopolyphosphatase genes from Pseudomonas aeruginosa 8830. Appl Environ Microbiol. 1999;65:2065-71 pubmed
    ..However, no coregulation between ppk and ppx promoters has been demonstrated in response to osmotic shock or oxidative stress. ..
  14. Smith L, Gomez M, Shatalin K, Lee H, Neyfakh A. Monitoring of gene knockouts: genome-wide profiling of conditionally essential genes. Genome Biol. 2007;8:R87 pubmed
    ..Our findings suggest that MGK can serve as a robust tool in functional genomics studies. ..
  15. Boyd A, Ghosh M, May T, Shinabarger D, Keogh R, Chakrabarty A. Sequence of the algL gene of Pseudomonas aeruginosa and purification of its alginate lyase product. Gene. 1993;131:1-8 pubmed
    ..Thus, the algL gene is located within a region of the alginate biosynthetic gene cluster that appears to be non-essential for alginate production. ..
  16. Helm R, Barnhart M, Seifert H. pilQ Missense mutations have diverse effects on PilQ multimer formation, piliation, and pilus function in Neisseria gonorrhoeae. J Bacteriol. 2007;189:3198-207 pubmed
    ..The collection of phenotypes expressed by these mutants suggests that PilQ has an active role in pilus expression and function...
  17. Liu S, Ogretmen B, Chuang Y, Stark B. Selection and characterization of alpha-amylase-overproducing recombinant Escherichia coli containing the bacterial hemoglobin gene. Appl Microbiol Biotechnol. 1992;38:239-42 pubmed
    ..The two latter results may be of particular importance in determining the way in which hemoglobin enhances the production of cloned protein products in recombinant bacteria...
  18. Shinabarger D, May T, Boyd A, Ghosh M, Chakrabarty A. Nucleotide sequence and expression of the Pseudomonas aeruginosa algF gene controlling acetylation of alginate. Mol Microbiol. 1993;9:1027-35 pubmed
    ..We show that a plasmid containing both the algF and algA gene complements the alginate acetylation defect of the algF mutant strain. ..
  19. O Connell W, Bangsborg J, Cianciotto N. Characterization of a Legionella micdadei mip mutant. Infect Immun. 1995;63:2840-5 pubmed
    ..Similarly, the mutant was less able to parasitize Hartmannella amoebae. Taken together, these data argue that Mip specifically potentiates intracellular growth by L. micdadei...
  20. Schlictman D, Shankar S, Chakrabarty A. The Escherichia coli genes sspA and rnk can functionally replace the Pseudomonas aeruginosa alginate regulatory gene algR2. Mol Microbiol. 1995;16:309-20 pubmed
    ..aeruginosa algR2 mutant. Furthermore, rnk was able to restore growth of the algR2 mutant in the presence of Tween 20, which inhibits other Ndk-like activities...
  21. Sato Y, Kuramitsu H. Sequence analysis of the Streptococcus mutans scrB gene. Infect Immun. 1988;56:1956-60 pubmed
    ..In addition, a region of amino acid homology with the S. mutans fructosyltransferase and B. subtilis levansucrase proteins was also detected. ..
  22. Castillo J, Greenberg J. Evolutionary dynamics of Ralstonia solanacearum. Appl Environ Microbiol. 2007;73:1225-38 pubmed
    ..Finally, genes essential for species survival are under purifying selection, and those directly involved in pathogenesis might be under diversifying selection...
  23. Silver S. Bacterial silver resistance: molecular biology and uses and misuses of silver compounds. FEMS Microbiol Rev. 2003;27:341-53 pubmed
    ..Ag is generally without adverse effects for humans, and argyria (irreversible discoloration of the skin resulting from subepithelial silver deposits) is rare and mostly of cosmetic concern...
  24. Hui F, Morrison D. Genetic transformation in Streptococcus pneumoniae: nucleotide sequence analysis shows comA, a gene required for competence induction, to be a member of the bacterial ATP-dependent transport protein family. J Bacteriol. 1991;173:372-81 pubmed
    ..These similarities suggest that ComA functions in the transport of some molecule, possibly pneumococcal competence factor itself...
  25. Roa B, Connolly D, Winkler M. Overlap between pdxA and ksgA in the complex pdxA-ksgA-apaG-apaH operon of Escherichia coli K-12. J Bacteriol. 1989;171:4767-77 pubmed
    ..The results are also considered in terms of ksgA expression. ..
  26. Ton That H, Schneewind O. Assembly of pili in Gram-positive bacteria. Trends Microbiol. 2004;12:228-34 pubmed
  27. Dogan I, Pagilla K, Webster D, Stark B. Expression of Vitreoscilla hemoglobin in Gordonia amarae enhances biosurfactant production. J Ind Microbiol Biotechnol. 2006;33:693-700 pubmed
    ..4-fold and 2.4-fold for limited and normal aeration, respectively. The engineered strain may be an improved source for producing purified biosurfactant or an aid to microorganisms bioremediating sparingly soluble contaminants in situ...
  28. Silver S, Phung L, Silver G. Silver as biocides in burn and wound dressings and bacterial resistance to silver compounds. J Ind Microbiol Biotechnol. 2006;33:627-34 pubmed
    ..These are beneficial products needing further study, although each has drawbacks. The genes (and proteins) involved in bacterial resistance to Ag have been defined and studied in recent years...
  29. Reeves A, Brikun I, Cernota W, Leach B, Gonzalez M, Weber J. Effects of methylmalonyl-CoA mutase gene knockouts on erythromycin production in carbohydrate-based and oil-based fermentations of Saccharopolyspora erythraea. J Ind Microbiol Biotechnol. 2006;33:600-9 pubmed
  30. Ahmed M, Borsch C, Taylor S, Vazquez Laslop N, Neyfakh A. A protein that activates expression of a multidrug efflux transporter upon binding the transporter substrates. J Biol Chem. 1994;269:28506-13 pubmed
  31. Mazmanian S, Skaar E, Gaspar A, Humayun M, Gornicki P, Jelenska J, et al. Passage of heme-iron across the envelope of Staphylococcus aureus. Science. 2003;299:906-9 pubmed publisher
    ..Thus, Isd appears to act as an import apparatus that uses cell wall-anchored proteins to relay heme-iron across the bacterial envelope...
  32. Shen F, Du W, Davydova E, Karymov M, Pandey J, Ismagilov R. Nanoliter multiplex PCR arrays on a SlipChip. Anal Chem. 2010;82:4606-12 pubmed publisher
    ..The SlipChip will be useful for applications involving PCR arrays and lays the foundation for new strategies for diagnostics, point-of-care devices, and immobilization-based arrays. ..
  33. Nichols B, Green J. Cloning and sequencing of Escherichia coli ubiC and purification of chorismate lyase. J Bacteriol. 1992;174:5309-16 pubmed
    ..Of the several enzymes that convert chorismate to intermediates of E. coli biosynthetic pathways, chorismate lyase is the last to be isolated and characterized. ..
  34. Nies A, Nies D, Silver S. Nucleotide sequence and expression of a plasmid-encoded chromate resistance determinant from Alcaligenes eutrophus. J Biol Chem. 1990;265:5648-53 pubmed
  35. Shinabarger D, Berry A, May T, Rothmel R, Fialho A, Chakrabarty A. Purification and characterization of phosphomannose isomerase-guanosine diphospho-D-mannose pyrophosphorylase. A bifunctional enzyme in the alginate biosynthetic pathway of Pseudomonas aeruginosa. J Biol Chem. 1991;266:2080-8 pubmed
    ..DNA sequencing of a cloned mutant algA gene from P. aeruginosa revealed that a point mutation at nucleotide 961 greatly decreased the levels of both PMI and GMP in a crude extract. ..
  36. Lee J, Edwards C, Hulett F. Bacillus licheniformis APase I gene promoter: a strong well-regulated promoter in B. subtilis. J Gen Microbiol. 1991;137:1127-33 pubmed
    ..Heterologous expression studies of the promoter using a fusion with the lacZ gene indicated that it functions as a very strong inducible promoter in B. subtilis that is tightly regulated by phosphate concentration. ..
  37. Robey M, Cianciotto N. Legionella pneumophila feoAB promotes ferrous iron uptake and intracellular infection. Infect Immun. 2002;70:5659-69 pubmed
    ..These data represent the first evidence for the importance of ferrous iron transport for intracellular replication by a human pathogen...
  38. Lee J, Edwards C, Hulett F. Identification of four unique clones encoding 10 kDa proteins from Bacillus that cause phenotypic complementation of a phoA mutant strain of Escherichia coli. J Gen Microbiol. 1991;137:667-77 pubmed
    ..It is clear that phenotypic complementation with cloned DNA from another genus does not ensure the identification of an APase gene. Possible mechanisms for the abnormal phenotypic complementation are discussed. ..
  39. Schoenlein P, Roa B, Winkler M. Divergent transcription of pdxB and homology between the pdxB and serA gene products in Escherichia coli K-12. J Bacteriol. 1989;171:6084-92 pubmed
    ..The homology between PdxB and SerA provides considerable support for Jensen's model of enzyme recruitment as the basis for the evolution of different biosynthetic pathways. ..
  40. Stark B, Urgun Demirtas M, Pagilla K. Role of hemoglobin in improving biodegradation of aromatic contaminants under hypoxic conditions. J Mol Microbiol Biotechnol. 2008;15:181-9 pubmed publisher
    ..Although not always the case, for the most part, and particularly in bioreactors, the advantages due to vgb/VHb were greater under conditions of limited aeration or hypoxic conditions...
  41. Gr ndling A, Missiakas D, Schneewind O. Staphylococcus aureus mutants with increased lysostaphin resistance. J Bacteriol. 2006;188:6286-97 pubmed publisher
    ..Other lyr mutants with intermediate lysostaphin resistance carried bursa aurealis insertions in genes specifying GTP pyrophosphokinase or enzymes of the purine biosynthetic pathway...
  42. Kato J, Misra T, Chakrabarty A. AlgR3, a protein resembling eukaryotic histone H1, regulates alginate synthesis in Pseudomonas aeruginosa. Proc Natl Acad Sci U S A. 1990;87:2887-91 pubmed
    ..coli demonstrate the presence of a 39-kDa polypeptide encoded by the cloned algR3 gene. ..
  43. Lee J, Hulett F. Nucleotide sequence of the phoP gene encoding PhoP, the response regulator of the phosphate regulon of Bacillus subtilis. Nucleic Acids Res. 1992;20:5848 pubmed
  44. Hendrickson W, Rudd K. Physical map location of the argFGH operon of Escherichia coli. J Bacteriol. 1992;174:3836-7 pubmed
  45. Ballicora M, Erben E, Yazaki T, Bertolo A, Demonte A, Schmidt J, et al. Identification of regions critically affecting kinetics and allosteric regulation of the Escherichia coli ADP-glucose pyrophosphorylase by modeling and pentapeptide-scanning mutagenesis. J Bacteriol. 2007;189:5325-33 pubmed
    ..We postulate this region as critical for the allosteric regulation of the enzyme, participating in the communication between the catalytic and regulatory domains. ..
  46. Yoon K, Silver S. A second gene in the Staphylococcus aureus cadA cadmium resistance determinant of plasmid pI258. J Bacteriol. 1991;173:7636-42 pubmed
    ..The cadC gene is needed in addition to cadA for full resistance to cadmium in S. aureus and Bacillus subtilis. cadC functions both in cis and in trans...
  47. Bookstein C, Edwards C, Kapp N, Hulett F. The Bacillus subtilis 168 alkaline phosphatase III gene: impact of a phoAIII mutation on total alkaline phosphatase synthesis. J Bacteriol. 1990;172:3730-7 pubmed
    ..The APase III gene has been mapped to approximately 50 degrees on the B. subtilis chromosome. ..
  48. Allard K, Viswanathan V, Cianciotto N. lbtA and lbtB are required for production of the Legionella pneumophila siderophore legiobactin. J Bacteriol. 2006;188:1351-63 pubmed
    ..The discovery of lbtAB represents the first identification of genes required for L. pneumophila siderophore expression...
  49. Kapp N, Edwards C, Chesnut R, Hulett F. The Bacillus subtilis phoAIV gene: effects of in vitro inactivation on total alkaline phosphatase production. Gene. 1990;96:95-100 pubmed
    ..There was no effect on the production of sporulation APase. The phoAIV::pJM103 insertion was mapped and determined to be located at approx. 73 degrees on the B. subtilis 360 degrees chromosome. ..
  50. Malakooti J, Komeda Y, Matsumura P. DNA sequence analysis, gene product identification, and localization of flagellar motor components of Escherichia coli. J Bacteriol. 1989;171:2728-34 pubmed
    ..In addition, we subcloned a 2.2-kilobase-pair DNA fragment capable of complementing the remaining four genes of the flaA operon (flbD [fliO], flaR [fliP], flaQ [fliQ], and flaP [fliR]). ..
  51. Shiroza T, Kuramitsu H. Sequence analysis of the Streptococcus mutans fructosyltransferase gene and flanking regions. J Bacteriol. 1988;170:810-6 pubmed
    ..In addition, ORF 3 is transcribed from the ftf DNA coding strand. Partial sequencing of ORF 4 suggests that its gene product may be an extracellular protein. ..
  52. Jones K, Buikema W, Haselkorn R. Heterocyst-specific expression of patB, a gene required for nitrogen fixation in Anabaena sp. strain PCC 7120. J Bacteriol. 2003;185:2306-14 pubmed
  53. Robey M, O Connell W, Cianciotto N. Identification of Legionella pneumophila rcp, a pagP-like gene that confers resistance to cationic antimicrobial peptides and promotes intracellular infection. Infect Immun. 2001;69:4276-86 pubmed
    ..However, in low-Mg(2+) medium, the rcp mutant was impaired in stationary-phase survival. This is the first demonstration of a pagP-like gene, involved in resistance to CAMPs, being required for intracellular infection and virulence. ..