Genomes and Genes
Experts and Doctors on protein binding in Miami, Florida, United States
Locale: Miami, Florida, United States
Topic: protein binding
Publications112 found, 100 shown here
- Mirsaeidi M, Gidfar S, Vu A, Schraufnagel D. Annexins family: insights into their functions and potential role in pathogenesis of sarcoidosis. J Transl Med. 2016;14:89 pubmed publisher..The mechanism of action of annexin A11 may be based upon alterations in delivering calcium to two different apoptosis pathways (caspase and P53). ..
- Flynn P, Helfman D. Non-muscle myosin IIB helps mediate TNF cell death signaling independent of actomyosin contractility (AMC). J Cell Biochem. 2010;110:1365-75 pubmed publisher..These findings suggest that NM IIB promotes TNF cell death signaling in a manner independent of its force generating property. ..
- McDonald C, El Hokayem J, Zafar N, Balke J, Bhat V, Mikles D, et al. Allostery mediates ligand binding to Grb2 adaptor in a mutually exclusive manner. J Mol Recognit. 2013;26:92-103 pubmed publisher..Collectively, our study sheds new light on the role of allostery in mediating cellular signaling machinery. ..
- Salas P. Insoluble gamma-tubulin-containing structures are anchored to the apical network of intermediate filaments in polarized CACO-2 epithelial cells. J Cell Biol. 1999;146:645-58 pubmed..Drastic changes in the extraction protocol did not affect coimmunoprecipitation. These results from three independent techniques, indicate that insoluble gamma-tubulin-containing structures are attached to apical intermediate filaments. ..
- Ramsauer V, Carraway C, Salas P, Carraway K. Muc4/sialomucin complex, the intramembrane ErbB2 ligand, translocates ErbB2 to the apical surface in polarized epithelial cells. J Biol Chem. 2003;278:30142-7 pubmed..The ability of Muc4/SMC to alter the localization of ErbB2, particularly a phosphorylated form of it, in epithelial cells, suggests that it has an important role in regulating ErbB2 signaling. ..
- Margolles Clark E, Kenyon N, Ricordi C, Buchwald P. Effective and specific inhibition of the CD40-CD154 costimulatory interaction by a naphthalenesulphonic acid derivative. Chem Biol Drug Des. 2010;76:305-13 pubmed publisher..There is now sufficient structure-activity relationship information to serve as the basis of a drug discovery initiative targeting this important costimulatory interaction. ..
- Bourens M, Dabir D, Tienson H, Sorokina I, Koehler C, Barrientos A. Role of twin Cys-Xaa9-Cys motif cysteines in mitochondrial import of the cytochrome C oxidase biogenesis factor Cmc1. J Biol Chem. 2012;287:31258-69 pubmed publisher..The three proteins are shown to form a ternary complex in mitochondria. Our results suggest that this mechanism facilitates efficient formation of multiple disulfides and prevents the formation of non-native disulfide bonds. ..
- Liang W, Deutscher M. Transfer-messenger RNA-SmpB protein regulates ribonuclease R turnover by promoting binding of HslUV and Lon proteases. J Biol Chem. 2012;287:33472-9 pubmed publisher..RNase R in stationary phase or in cold-shocked cells is not acetylated, and thereby remains stable. Such a regulatory mechanism, dependent on protein acetylation, has not been observed previously in bacterial cells...
- Sandiford S, Slepak V. The Gbeta5-RGS7 complex selectively inhibits muscarinic M3 receptor signaling via the interaction between the third intracellular loop of the receptor and the DEP domain of RGS7. Biochemistry. 2009;48:2282-9 pubmed publisher..These results identify a novel molecular mechanism that can impart receptor subtype selectivity on signal transduction via Gq-coupled muscarinic receptors. ..
- Szczesna D, Ghosh D, Li Q, Gomes A, Guzman G, Arana C, et al. Familial hypertrophic cardiomyopathy mutations in the regulatory light chains of myosin affect their structure, Ca2+ binding, and phosphorylation. J Biol Chem. 2001;276:7086-92 pubmed
- Tao Z, Zhang Z, Grewer C. Neutralization of the aspartic acid residue Asp-367, but not Asp-454, inhibits binding of Na+ to the glutamate-free form and cycling of the glutamate transporter EAAC1. J Biol Chem. 2006;281:10263-72 pubmed..Our data are consistent with a model in which Asp-367, but not Asp-454, is involved in coordinating the bound Na(+) in the glutamate-free transporter form. ..
- Baker A, Fu Q, Hayward W, Victoria S, Pedroso I, Lindsay S, et al. The telomere binding protein TRF2 induces chromatin compaction. PLoS ONE. 2011;6:e19124 pubmed publisher..Our data suggest that nucleosomal chromatin has the ability to facilitate this activity of TRF2 which is thought to be involved in stabilizing looped telomere structures. ..
- SOTO I, Fontanesi F, Myers R, Hamel P, Barrientos A. A heme-sensing mechanism in the translational regulation of mitochondrial cytochrome c oxidase biogenesis. Cell Metab. 2012;16:801-13 pubmed publisher..We conclude that heme sensing by Mss51 regulates COX biogenesis and aerobic energy production. ..
- Bartra S, Ding Y, Fujimoto L, Ring J, Jain V, Ram S, et al. Yersinia pestis uses the Ail outer membrane protein to recruit vitronectin. Microbiology. 2015;161:2174-83 pubmed publisher..Escherichia coli DH5Î± expressing Y. pestis Ail bound vitronectin, but not heat-treated vitronectin. The ability of Ail to directly bind vitronectin was demonstrated by ELISA using purified refolded Ail in nanodiscs. ..
- Khan S, Yanez G, Seldeen K, Wang H, Lindsay S, Fletcher T. Interactions of TRF2 with model telomeric ends. Biochem Biophys Res Commun. 2007;363:44-50 pubmed..We suggest that T-loop formation at telomere ends involves TRF2 binding to the G-strand overhang and oligomerization through both the known TRFH domain and the linker region. ..
- Shembade N, Parvatiyar K, Harhaj N, Harhaj E. The ubiquitin-editing enzyme A20 requires RNF11 to downregulate NF-kappaB signalling. EMBO J. 2009;28:513-22 pubmed publisher..Our studies reveal that RNF11, together with TAX1BP1 and Itch, is an essential component of an A20 ubiquitin-editing protein complex that ensures transient activation of inflammatory signalling pathways. ..
- Schmid A, Bai G, Schmid N, Zaccolo M, Ostrowski L, Conner G, et al. Real-time analysis of cAMP-mediated regulation of ciliary motility in single primary human airway epithelial cells. J Cell Sci. 2006;119:4176-86 pubmed
- Eibl R, Moy V. Atomic force microscopy measurements of protein-ligand interactions on living cells. Methods Mol Biol. 2005;305:439-50 pubmed..This protocol is also suitable for studying single-molecule de-adhesion events in cell-free systems as well as between two living cells. ..
- Nair K, Hanson S, Mendez A, Gurevich E, Kennedy M, Shestopalov V, et al. Light-dependent redistribution of arrestin in vertebrate rods is an energy-independent process governed by protein-protein interactions. Neuron. 2005;46:555-67 pubmed..These results indicate that the distribution of arrestin in rods is controlled by its dynamic interactions with rhodopsin in the OS and microtubules in the IS and that its movement occurs by simple diffusion...
- Rao S, Lyons L, Fahrenholtz C, Wu F, Farooq A, Balkan W, et al. A novel nuclear role for the Vav3 nucleotide exchange factor in androgen receptor coactivation in prostate cancer. Oncogene. 2012;31:716-27 pubmed publisher..These data demonstrate the importance of Vav3 in CRPC and define a novel nuclear function of Vav3 in regulating AR activity. ..
- Bourguignon L, Zhu H, Shao L, Chen Y. CD44 interaction with tiam1 promotes Rac1 signaling and hyaluronic acid-mediated breast tumor cell migration. J Biol Chem. 2000;275:1829-38 pubmed..Taken together, we believe that the linkage between CD44v3 isoform and the PHn-CC-EX domain of Tiam1 is required for HA stimulated Rac1 signaling and cytoskeleton-mediated tumor cell migration during breast cancer progression. ..
- Blair Johnson M, Fiedler T, Fenna R. Human myeloperoxidase: structure of a cyanide complex and its interaction with bromide and thiocyanate substrates at 1.9 A resolution. Biochemistry. 2001;40:13990-7 pubmed..These results support a model for a single common binding site for halides and thiocyanate as substrates or as inhibitors near the delta-meso carbon of the porphyrin ring in myeloperoxidase...
- Rao C. Potential Therapy for Neisseria Gonorrhoeae Infections With Human Chorionic Gonadotropin. Reprod Sci. 2015;22:1484-7 pubmed publisher..Based on these data, we suggest testing hCG in clinical trials on infected women. ..
- Aguero T, Jin Z, Chorghade S, Kalsotra A, King M, Yang J. Maternal Dead-end 1 promotes translation of nanos1 by binding the eIF3 complex. Development. 2017;144:3755-3765 pubmed publisher..This work uncovers a novel translational regulatory mechanism that is fundamentally important for germline development. ..
- Ofengand J, Del Campo M, Kaya Y. Mapping pseudouridines in RNA molecules. Methods. 2001;25:365-73 pubmed..A poly(A) tailing procedure is described that allows extension of the method to within a few nucleotides of the 3' terminus. ..
- Horn D, Al Ali H, Barrientos A. Cmc1p is a conserved mitochondrial twin CX9C protein involved in cytochrome c oxidase biogenesis. Mol Cell Biol. 2008;28:4354-64 pubmed publisher..Our results suggest that Cmc1p could play a direct or indirect role in copper trafficking and distribution to COX and Sod1p. ..
- Ross J, Plano G. A C-terminal region of Yersinia pestis YscD binds the outer membrane secretin YscC. J Bacteriol. 2011;193:2276-89 pubmed publisher..Finally, replacement of the YscD TM domain with a TM domain of dissimilar sequence had no effect on the T3S process, indicating that the TM domain has no sequence-specific function in the assembly or function of the T3SS. ..
- Liu C, Balsamo V, Sun D, Naja M, Wang X, Rosen B, et al. A 3D localized surface plasmon resonance biosensor for the study of trivalent arsenic binding to the ArsA ATPase. Biosens Bioelectron. 2012;38:19-26 pubmed publisher..Binding of As(III) to ArsA is greatly facilitated by the presence of magnesium ion and ATP. ..
- Enninga J, Levay A, Fontoura B. Sec13 shuttles between the nucleus and the cytoplasm and stably interacts with Nup96 at the nuclear pore complex. Mol Cell Biol. 2003;23:7271-84 pubmed..These results indicate that Sec13 stably interacts with Nup96 at the NPC during interphase and that the shuttling of Sec13 between the nucleus and the cytoplasm may couple and regulate functions between these two compartments. ..
- Marrero E, Rossi S, Darr A, Tsoulfas P, Rotundo R. Translational regulation of acetylcholinesterase by the RNA-binding protein Pumilio-2 at the neuromuscular synapse. J Biol Chem. 2011;286:36492-9 pubmed publisher..Finally, we found that PUM2 is regulated by the motor nerve suggesting a trans-synaptic mechanism for locally regulating translation of specific proteins involved in modulating synaptic transmission, analogous to CNS synapses. ..
- Diez Sampedro A, Barcelona S. Sugar binding residue affects apparent Na+ affinity and transport stoichiometry in mouse sodium/glucose cotransporter type 3B. J Biol Chem. 2011;286:7975-82 pubmed publisher..This model explains the uncoupled charge:sugar transport phenotype observed in wild type and G457E-mSGLT3b compared with SGLT1 and the sugar-activated cation transport without sugar transport that occurs in hSGLT3. ..
- Silva Herzog E, Joseph S, Avery A, Coba J, Wolf K, Fields K, et al. Scc1 (CP0432) and Scc4 (CP0033) function as a type III secretion chaperone for CopN of Chlamydia pneumoniae. J Bacteriol. 2011;193:3490-6 pubmed publisher..The Scc1/Scc4 chaperone promoted the efficient secretion of CopN via a heterologous T3SS, whereas, the Scc3 chaperone, which binds to a C-terminal region of CopN, reduced CopN secretion. ..
- Goel M, Sienkiewicz A, Picciani R, Lee R, Bhattacharya S. Cochlin induced TREK-1 co-expression and annexin A2 secretion: role in trabecular meshwork cell elongation and motility. PLoS ONE. 2011;6:e23070 pubmed publisher..Annexin A2 expression and secretion follows cochlin-TREK-1 syn-expression and correlates with cell elongation. Thus cytoskeleton changes in response to fluid shear sensed by cochlin are further mediated by TREK-1 and annexin A2. ..
- Vargas N, Alvarez Cubela S, Giraldo J, Nieto M, Fort N, Cechin S, et al. TAT-mediated transduction of MafA protein in utero results in enhanced pancreatic insulin expression and changes in islet morphology. PLoS ONE. 2011;6:e22364 pubmed publisher..To our knowledge, this is also the first report on the use of protein transduction in utero. ..
- Frasca D, Van der Put E, Riley R, Blomberg B. Reduced Ig class switch in aged mice correlates with decreased E47 and activation-induced cytidine deaminase. J Immunol. 2004;172:2155-62 pubmed..Our studies show that impaired induction of E47, and subsequently activation-induced cytidine deaminase, contribute to poor CSR and production of secondary isotypes in senescence. ..
- Espert S, Elsinghorst E, Munson G. The tib adherence locus of enterotoxigenic Escherichia coli is regulated by cyclic AMP receptor protein. J Bacteriol. 2011;193:1369-76 pubmed publisher..Thus, these studies demonstrate that CRP positively regulates the expression of the glycosylated afimbrial adhesin TibA through occupancy of a binding site within tibDBp. ..
- Witherow D, Slepak V. A novel kind of G protein heterodimer: the G beta5-RGS complex. Receptors Channels. 2003;9:205-12 pubmed..This review summarizes the information about the assembly and function of G beta5-RGS dimers, as well as their posttranslational modifications and localization. ..
- Yuan F, El Hokayem J, Zhou W, Zhang Y. FANCI protein binds to DNA and interacts with FANCD2 to recognize branched structures. J Biol Chem. 2009;284:24443-52 pubmed publisher..Our data suggest that the FANCI-FANCD2 complex may participate in repair of damaged replication forks through its preferential recognition of branched structures. ..
- Vincent H, Deutscher M. Substrate recognition and catalysis by the exoribonuclease RNase R. J Biol Chem. 2006;281:29769-75 pubmed..Based on these data and a homology model of RNase R, derived from the structure of the closely related enzyme, RNase II, we present a model for how RNase R interacts with its substrates and degrades RNA. ..
- Abaffy T, Malhotra A, Luetje C. The molecular basis for ligand specificity in a mouse olfactory receptor: a network of functionally important residues. J Biol Chem. 2007;282:1216-24 pubmed..Our results demonstrate a powerful approach based on the synergy between computational predictions and physiological assays. ..
- Kapiloff M, Piggott L, Sadana R, Li J, Heredia L, Henson E, et al. An adenylyl cyclase-mAKAPbeta signaling complex regulates cAMP levels in cardiac myocytes. J Biol Chem. 2009;284:23540-6 pubmed publisher..Taken together, these results show that the association of AC5 with the mAKAPbeta complex is required for the regulation of cAMP second messenger controlling cardiac myocyte hypertrophy. ..
- Nakamura R, Hackam A. Analysis of Dickkopf3 interactions with Wnt signaling receptors. Growth Factors. 2010;28:232-42 pubmed publisher..Additionally, Krm2 abolished Dkk3-mediated potentiation of Wnt signaling. Therefore, our data establish that Krm proteins are novel binding partners of Dkk3 and suggest a mechanism by which Dkk3 potentiates Wnt signaling. ..
- Zhang L, Lubin A, Chen H, Sun Z, Gong F. The deubiquitinating protein USP24 interacts with DDB2 and regulates DDB2 stability. Cell Cycle. 2012;11:4378-84 pubmed publisher..In addition, we demonstrated that USP24 can cleave an ubiquitinated form of DDB2 in vitro. Taken together, our results suggest that the ubiquitin-specific protease USP24 is a novel regulator of DDB2 stability. ..
- Al Ali H, Ding Y, Slepak T, Wu W, Sun Y, Martinez Y, et al. The mTOR Substrate S6 Kinase 1 (S6K1) Is a Negative Regulator of Axon Regeneration and a Potential Drug Target for Central Nervous System Injury. J Neurosci. 2017;37:7079-7095 pubmed publisher..We provide mechanistic evidence that S6K1's negative feedback to PI3K signaling is involved in axon growth inhibition, and show that phosphorylation of S6K1 is a more appropriate regeneration indicator than is S6 phosphorylation. ..
- Forteza R, Casalino Matsuda S, Monzon M, Fries E, Rugg M, Milner C, et al. TSG-6 potentiates the antitissue kallikrein activity of inter-alpha-inhibitor through bikunin release. Am J Respir Cell Mol Biol. 2007;36:20-31 pubmed..e., pre-alpha-inhibitor) were also induced by TNF-alpha in primary cultures. Our results suggest that TSG-6 may play an important protective role in bronchial epithelium by increasing the antiprotease screen on the airway lumen. ..
- Chu I, Sun J, Arnaout A, Kahn H, Hanna W, Narod S, et al. p27 phosphorylation by Src regulates inhibition of cyclin E-Cdk2. Cell. 2007;128:281-94 pubmed..Importantly, we report that in tamoxifen-resistant breast cancer cell lines, Src inhibition can increase p27 levels and restore tamoxifen sensitivity. These data provide a new rationale for Src inhibitors in cancer therapy. ..
- Galvis A, Giambini H, Villasana Z, Barbieri M. Functional determinants of ras interference 1 mutants required for their inhbitory activity on endocytosis. Exp Cell Res. 2009;315:820-35 pubmed publisher..Based on these data, our results showed that mutations in the Vps9 domain of Rin1 lead to a loss-of-function phenotype, indicating a specific structure-function relationship between Rab5 and Rin1. ..
- Caberoy N, Li W. Unconventional secretion of tubby and tubby-like protein 1. FEBS Lett. 2009;583:3057-62 pubmed publisher..Moreover, unconventional secretion of tubby and Tulp1 is independent of endoplasmic reticulum-Golgi pathway. These data implicate that Tulps may function extracellularly as well. ..
- McDonald C, McIntosh S, Mikles D, Bhat V, Deegan B, Seldeen K, et al. Biophysical analysis of binding of WW domains of the YAP2 transcriptional regulator to PPXY motifs within WBP1 and WBP2 adaptors. Biochemistry. 2011;50:9616-27 pubmed publisher..Collectively, our study sheds light on the molecular determinants of a key WW-ligand interaction pertinent to cellular functions in health and disease. ..
- Li J, Vargas M, Kapiloff M, Dodge Kafka K. Regulation of MEF2 transcriptional activity by calcineurin/mAKAP complexes. Exp Cell Res. 2013;319:447-54 pubmed publisher..Together these data illustrate the importance of calcineurin anchoring by the mAKAP scaffold for MEF2 regulation. ..
- De Silva D, Fontanesi F, Barrientos A. The DEAD box protein Mrh4 functions in the assembly of the mitochondrial large ribosomal subunit. Cell Metab. 2013;18:712-25 pubmed publisher..We conclude that Mrh4 plays an essential role during the late stages of mitoribosome assembly by promoting remodeling of the 21S rRNA-protein interactions. ..
- Zuo Y, Deutscher M. Mechanism of action of RNase T. I. Identification of residues required for catalysis, substrate binding, and dimerization. J Biol Chem. 2002;277:50155-9 pubmed..These data define the domains necessary for RNase T action, and together with information in the accompanying article, have led to the formulation of a detailed model for the structure and mechanism of action of RNase T. ..
- Jy W, Jimenez J, Mauro L, Horstman L, Cheng P, Ahn E, et al. Endothelial microparticles induce formation of platelet aggregates via a von Willebrand factor/ristocetin dependent pathway, rendering them resistant to dissociation. J Thromb Haemost. 2005;3:1301-8 pubmed..Multimer analysis of vWf on EMP showed unusually large vWf (ULvWf). In summary, EMP carries ULvWf multimers, promote platelet aggregates, and increase the stability of the aggregates thus formed. ..
- Qiu F, Rebolledo S, Gonzalez C, Larsson H. Subunit interactions during cooperative opening of voltage-gated proton channels. Neuron. 2013;77:288-98 pubmed publisher..These observations suggest that, following an initial S4 charge movement in the two subunits, there is a second, cooperative conformational change, involving interactions between subunits, that opens both pathways in Hv1 channels. ..
- Yanez G, Khan S, Locovei A, Pedroso I, Fletcher T. DNA structure-dependent recruitment of telomeric proteins to single-stranded/double-stranded DNA junctions. Biochem Biophys Res Commun. 2005;328:49-56 pubmed..This suggests that an intramolecular G-quadruplex has the potential to disrupt certain telomeric assemblies, but efficient recruitment of appropriate DNA repair proteins provides the means to overcome this obstacle. ..
- Oriolo A, Wald F, Canessa G, Salas P. GCP6 binds to intermediate filaments: a novel function of keratins in the organization of microtubules in epithelial cells. Mol Biol Cell. 2007;18:781-94 pubmed..We conclude that GCP6 participates in the attachment of MTOCs to IFs in epithelial cells and is among the factors that determine the peculiar architecture of microtubules in polarized epithelia. ..
- Boettner D, Friesen H, Andrews B, Lemmon S. Clathrin light chain directs endocytosis by influencing the binding of the yeast Hip1R homologue, Sla2, to F-actin. Mol Biol Cell. 2011;22:3699-714 pubmed publisher..We propose that CLC directs endocytic progression by pruning the Sla2-actin attachments in the clathrin lattice, providing direction for membrane internalization. ..
- Gao L, Harhaj E. HSP90 protects the human T-cell leukemia virus type 1 (HTLV-1) tax oncoprotein from proteasomal degradation to support NF-?B activation and HTLV-1 replication. J Virol. 2013;87:13640-54 pubmed publisher..Taken together, our results reveal that Tax is a novel HSP90 client protein and HSP90 inhibitors may exert therapeutic benefits for ATL and HAM/TSP patients...
- Loor G, Zhang S, Zhang P, Toomey N, Lee M. Identification of DNA replication and cell cycle proteins that interact with PCNA. Nucleic Acids Res. 1997;25:5041-6 pubmed..Of the cell cycle proteins investigated, CDK2, CDK4 and CDK5 were bound. This study presents strong evidence that PCNA is a component of protein complexes containing DNA replication, repair and cell cycle regulatory proteins. ..
- Guo Y, Guettouche T, Fenna M, Boellmann F, Pratt W, Toft D, et al. Evidence for a mechanism of repression of heat shock factor 1 transcriptional activity by a multichaperone complex. J Biol Chem. 2001;276:45791-9 pubmed..This repression mechanism may be required for a proportional transcriptional response to stress. Formation of the heterocomplex may also represent the first step toward returning the hHSF1 to its unactivated form. ..
- Carastro L, Tan C, Selg M, Jack H, So A, Downey K. Identification of delta helicase as the bovine homolog of HUPF1: demonstration of an interaction with the third subunit of DNA polymerase delta. Nucleic Acids Res. 2002;30:2232-43 pubmed..Immunoprecipitation experiments demonstrated that HUPF1 interacts with the 66-kDa third subunit of pol delta in vivo...
- Hunker C, Galvis A, Kruk I, Giambini H, Veisaga M, Barbieri M. Rab5-activating protein 6, a novel endosomal protein with a role in endocytosis. Biochem Biophys Res Commun. 2006;340:967-75 pubmed..This study is the first to show that RAP6 is a novel regulator of endocytosis that exhibits GEF activity specific for Rab5 and GAP activity specific for Ras. ..
- Liang W, Deutscher M. A novel mechanism for ribonuclease regulation: transfer-messenger RNA (tmRNA) and its associated protein SmpB regulate the stability of RNase R. J Biol Chem. 2010;285:29054-8 pubmed publisher..These data define a previously unknown regulatory process in which the stability of an RNase is determined by its interaction with an RNA and an RNA-associated protein. ..
- Campos M, Abreu A, Nlend M, Cobas M, Conner G, Whitney P. Purification and characterization of PLUNC from human tracheobronchial secretions. Am J Respir Cell Mol Biol. 2004;30:184-92 pubmed..This study showed that plunc gene product is expressed both in vivo and in vitro, detailed a method for its purification and provided basic information on its biochemical properties in secretions. ..
- Pilonieta M, Munson G. The chaperone IpgC copurifies with the virulence regulator MxiE. J Bacteriol. 2008;190:2249-51 pubmed publisher..In this paper, we report that the chaperone forms a specific and stable heteromer with MxiE...
- Vincent H, Deutscher M. The roles of individual domains of RNase R in substrate binding and exoribonuclease activity. The nuclease domain is sufficient for digestion of structured RNA. J Biol Chem. 2009;284:486-94 pubmed publisher..We also show that the nuclease domain of RNase R binds RNA more tightly than the nuclease domain of RNase II. This tighter binding may help to explain the difference in catalytic properties between RNase R and RNase II. ..
- Chen B, Young J, Leng F. DNA bending by the mammalian high-mobility group protein AT hook 2. Biochemistry. 2010;49:1590-5 pubmed publisher..The DNA bending angles were estimated to be 34.2 degrees , 33.5 degrees , and 35.4 degrees , respectively. ..
- Vasquez Del Carpio R, Kaplan F, Weaver K, VanWye J, Alves Guerra M, Robbins D, et al. Assembly of a Notch transcriptional activation complex requires multimerization. Mol Cell Biol. 2011;31:1396-408 pubmed publisher..These interactions form a preactivation complex that is then resolved by CSL to form the Notch transcriptional activation complex on DNA. ..
- Liang W, Malhotra A, Deutscher M. Acetylation regulates the stability of a bacterial protein: growth stage-dependent modification of RNase R. Mol Cell. 2011;44:160-6 pubmed publisher..These findings indicate that acetylation can regulate the stability of a bacterial protein. ..
- Mim C, Tao Z, Grewer C. Two conformational changes are associated with glutamate translocation by the glutamate transporter EAAC1. Biochemistry. 2007;46:9007-18 pubmed
- Bigford G, Chaudhry N, Keane R, Holohean A. 5-Hydroxytryptamine 5HT2C receptors form a protein complex with N-methyl-D-aspartate GluN2A subunits and activate phosphorylation of Src protein to modulate motoneuronal depolarization. J Biol Chem. 2012;287:11049-59 pubmed publisher..These findings reveal that a distinct multiprotein complex links 5-hydroxytryptamine-activated intracellular signaling events with NMDA-mediated functional activity. ..
- Madireddi S, Eun S, Mehta A, Birta A, Zajonc D, Niki T, et al. Regulatory T Cell-Mediated Suppression of Inflammation Induced by DR3 Signaling Is Dependent on Galectin-9. J Immunol. 2017;199:2721-2728 pubmed publisher..Similar results were seen in an allergic lung inflammation model. Thus, we demonstrate a novel function of Galectin-9 in facilitating activity of DR3 related to Treg-mediated suppression. ..
- Zuo Y, Deutscher M. Mechanism of action of RNase T. II. A structural and functional model of the enzyme. J Biol Chem. 2002;277:50160-4 pubmed..These data explain why RNase T must dimerize to function. The model provides a detailed framework on which to explain the mechanism of action of RNase T. ..
- Harhaj E, Harhaj N, Grant C, Mostoller K, Alefantis T, Sun S, et al. Human T cell leukemia virus type I Tax activates CD40 gene expression via the NF-kappa B pathway. Virology. 2005;333:145-58 pubmed..Collectively, these results indicate that CD40 is a novel Tax-regulated gene, and the regulation of CD40 by Tax may play a role in cellular activation and HTLV-I-induced disease pathogenesis. ..
- Seldeen K, McDonald C, Deegan B, Farooq A. Single nucleotide variants of the TGACTCA motif modulate energetics and orientation of binding of the Jun-Fos heterodimeric transcription factor. Biochemistry. 2009;48:1975-83 pubmed publisher..Our study also bears important consequences for the occurrence of single nucleotide polymorphisms within the TGACTCA cis element at specific gene promoters between different individuals. ..
- Vincent H, Deutscher M. Insights into how RNase R degrades structured RNA: analysis of the nuclease domain. J Mol Biol. 2009;387:570-83 pubmed publisher..On the basis of these data, we propose a model in which tight binding within the nuclease domain allows RNase R to capitalize on the natural thermal breathing of an RNA duplex to degrade structured RNAs. ..
- Jiang X, Lu X, McNamara G, Liu X, Cubedo E, Sarosiek K, et al. HGAL, a germinal center specific protein, decreases lymphoma cell motility by modulation of the RhoA signaling pathway. Blood. 2010;116:5217-27 pubmed publisher..This mechanism may underlie the limited dissemination and better outcome of patients with HGAL-expressing DLBCL and cHL. ..
- Wu F, Peacock S, Rao S, Lemmon S, Burnstein K. Novel interaction between the co-chaperone Cdc37 and Rho GTPase exchange factor Vav3 promotes androgen receptor activity and prostate cancer growth. J Biol Chem. 2013;288:5463-74 pubmed publisher..Taken together, we identified a novel Vav3 interacting protein that enhances Vav3 co-activation of AR and prostate cancer cell proliferation. Vav3-Cdc37 interaction may provide a new therapeutic target in prostate cancer. ..
- Wojcikiewicz E, Koenen R, Fraemohs L, Minkiewicz J, Azad H, Weber C, et al. LFA-1 binding destabilizes the JAM-A homophilic interaction during leukocyte transmigration. Biophys J. 2009;96:285-93 pubmed publisher..In turn, the greater strength of the LFA-1/JAM-A complex permits it to support the tension needed to disrupt the JAM-A homophilic interaction, thus allowing transendothelial migration to proceed. ..
- Collette J, Chi R, Boettner D, Fernandez Golbano I, Plemel R, Merz A, et al. Clathrin functions in the absence of the terminal domain binding site for adaptor-associated clathrin-box motifs. Mol Biol Cell. 2009;20:3401-13 pubmed publisher..These data suggest that additional or alternative binding sites exist on the TD propeller to help facilitate the recruitment of clathrin to sites of vesicle formation. ..
- Caberoy N, Maiguel D, Kim Y, Li W. Identification of tubby and tubby-like protein 1 as eat-me signals by phage display. Exp Cell Res. 2010;316:245-57 pubmed publisher..These data reveal a novel activity of tubby and Tulp1, and demonstrate that unbiased identification of eat-me signals by the broadly applicable strategy of ORF phage display can provide detailed insights into phagocyte biology. ..
- Caberoy N, Zhou Y, Li W. Tubby and tubby-like protein 1 are new MerTK ligands for phagocytosis. EMBO J. 2010;29:3898-910 pubmed publisher..PPBD was mapped to the highly conserved 54 amino acids at the C-terminal end of tubby and Tulp1. These data suggest that tubby and Tulp1 are novel bridging molecules to facilitate phagocytosis through MerTK. ..
- Marapakala K, Qin J, Rosen B. Identification of catalytic residues in the As(III) S-adenosylmethionine methyltransferase. Biochemistry. 2012;51:944-51 pubmed publisher..The rate-limiting step was identified as the conversion of DMAs(III) to trimethylarsine, and DMAs(III) accumulates as the principal product. ..