Experts and Doctors on green fluorescent proteins in Miami, Florida, United States

Summary

Locale: Miami, Florida, United States
Topic: green fluorescent proteins

Top Publications

  1. Abdulreda M, Faleo G, Molano R, Lopez Cabezas M, Molina J, Tan Y, et al. High-resolution, noninvasive longitudinal live imaging of immune responses. Proc Natl Acad Sci U S A. 2011;108:12863-8 pubmed publisher
  2. Koilkonda R, Hauswirth W, Guy J. Efficient expression of self-complementary AAV in ganglion cells of the ex vivo primate retina. Mol Vis. 2009;15:2796-802 pubmed
    ..Donated ex vivo primate eyes may serve as a model system for testing RGC expression before in vivo intravitreal injections of this and perhaps other AAV serotypes. ..
  3. Perea Martinez I, Nagai T, Chaudhari N. Functional cell types in taste buds have distinct longevities. PLoS ONE. 2013;8:e53399 pubmed publisher
    ..A small number of slow-cycling cells may also exist within the perimeter of the taste bud. Based on their incidence, we hypothesize that these may be progenitors for Type III cells. ..
  4. Yu H, Ozdemir S, Koilkonda R, Chou T, Porciatti V, Chiodo V, et al. Mutant NADH dehydrogenase subunit 4 gene delivery to mitochondria by targeting sequence-modified adeno-associated virus induces visual loss and optic atrophy in mice. Mol Vis. 2012;18:1668-83 pubmed
  5. Wiley D, Nordfeldth R, Rosenzweig J, DaFonseca C, Gustin R, Wolf Watz H, et al. The Ser/Thr kinase activity of the Yersinia protein kinase A (YpkA) is necessary for full virulence in the mouse, mollifying phagocytes, and disrupting the eukaryotic cytoskeleton. Microb Pathog. 2006;40:234-43 pubmed
    ..More generally, our finding that the 'eukaryotic-like' kinase domain of YpkA is important for virulence illustrates how a bacterium can utilize a host-like factor or activity in order to enhance its survival following host cell contact. ..
  6. Valledor M, Hu Q, Schiller P, Myers R. Fluorescent protein engineering by in vivo site-directed mutagenesis. IUBMB Life. 2012;64:684-9 pubmed publisher
    ..A set of rules to simplify mutant isolation by recombineering is provided. ..
  7. Pearse D, Sanchez A, Pereira F, Andrade C, Puzis R, Pressman Y, et al. Transplantation of Schwann cells and/or olfactory ensheathing glia into the contused spinal cord: Survival, migration, axon association, and functional recovery. Glia. 2007;55:976-1000 pubmed
  8. Soderblom C, Luo X, Blumenthal E, Bray E, Lyapichev K, Ramos J, et al. Perivascular fibroblasts form the fibrotic scar after contusive spinal cord injury. J Neurosci. 2013;33:13882-7 pubmed publisher
    ..Our results identify collagen1?1 cells as a novel source of the fibrotic scar after spinal cord injury and shift the focus from the meninges to the vasculature during scar formation. ..
  9. Williams R, Pearse D, Tresco P, Bunge M. The assessment of adeno-associated vectors as potential intrinsic treatments for brainstem axon regeneration. J Gene Med. 2012;14:20-34 pubmed publisher
    ..e. complete transection and cell implantation) should be used in combination with AAV-EGFP to directly assess AAV-mediated expression of therapeutic transgenes as intrinsic treatments to improve axonal regeneration. ..

More Information

Publications36

  1. Huang Y, Grant J, Roper S. Glutamate may be an efferent transmitter that elicits inhibition in mouse taste buds. PLoS ONE. 2012;7:e30662 pubmed publisher
    ..Indeed, glutamate-evoked 5-HT release inhibited taste-evoked ATP secretion. The findings are consistent with a role for glutamate in taste buds as an inhibitory efferent transmitter that acts via ionotropic synaptic glutamate receptors. ..
  2. Stepanek L, Stoker A, Stoeckli E, Bixby J. Receptor tyrosine phosphatases guide vertebrate motor axons during development. J Neurosci. 2005;25:3813-23 pubmed
    ..Our results indicate that RPTPs, and especially PTPRO, are required for axon growth and guidance in the developing vertebrate limb. ..
  3. Schaal S, Kitay B, Cho K, Lo T, Barakat D, Marcillo A, et al. Schwann cell transplantation improves reticulospinal axon growth and forelimb strength after severe cervical spinal cord contusion. Cell Transplant. 2007;16:207-28 pubmed
  4. Tomchik S, Berg S, Kim J, Chaudhari N, Roper S. Breadth of tuning and taste coding in mammalian taste buds. J Neurosci. 2007;27:10840-8 pubmed
  5. Rao S, Lyons L, Fahrenholtz C, Wu F, Farooq A, Balkan W, et al. A novel nuclear role for the Vav3 nucleotide exchange factor in androgen receptor coactivation in prostate cancer. Oncogene. 2012;31:716-27 pubmed publisher
    ..These data demonstrate the importance of Vav3 in CRPC and define a novel nuclear function of Vav3 in regulating AR activity. ..
  6. Mackenzie I, Nicholson A, Sarkar M, Messing J, Purice M, Pottier C, et al. TIA1 Mutations in Amyotrophic Lateral Sclerosis and Frontotemporal Dementia Promote Phase Separation and Alter Stress Granule Dynamics. Neuron. 2017;95:808-816.e9 pubmed publisher
    ..Moreover, TDP-43 in SGs became less mobile and insoluble. The identification of TIA1 mutations in ALS/FTD reinforces the importance of RNA metabolism and SG dynamics in ALS/FTD pathogenesis. ..
  7. Nedeau A, Gallagher K, Liu Z, Velazquez O. Elevation of hemopexin-like fragment of matrix metalloproteinase-2 tissue levels inhibits ischemic wound healing and angiogenesis. J Vasc Surg. 2011;54:1430-8 pubmed publisher
    ..Microscopic examination shows the wounds exhibit fewer capillaries, supporting the hypothesis that PEX2 decreases angiogenesis. ..
  8. Egea S, Dickerson I. Direct interactions between calcitonin-like receptor (CLR) and CGRP-receptor component protein (RCP) regulate CGRP receptor signaling. Endocrinology. 2012;153:1850-60 pubmed publisher
    ..To date, RCP has been found to interact only with CLR and represents a novel neuroendocrine regulatory step in GPCR signaling. ..
  9. Rodriguez Diaz R, Speier S, Molano R, Formoso A, Gans I, Abdulreda M, et al. Noninvasive in vivo model demonstrating the effects of autonomic innervation on pancreatic islet function. Proc Natl Acad Sci U S A. 2012;109:21456-61 pubmed publisher
    ..This study directly demonstrates that autonomic axons innervating the islet modulate glucose homeostasis...
  10. Koilkonda R, Yu H, Chou T, Feuer W, Ruggeri M, Porciatti V, et al. Safety and effects of the vector for the Leber hereditary optic neuropathy gene therapy clinical trial. JAMA Ophthalmol. 2014;132:409-20 pubmed publisher
  11. Trakhtenberg E, Wang Y, Morkin M, Fernandez S, Mlacker G, Shechter J, et al. Regulating Set-?'s Subcellular Localization Toggles Its Function between Inhibiting and Promoting Axon Growth and Regeneration. J Neurosci. 2014;34:7361-74 pubmed publisher
    ..Thus, Set-? differentially regulates axon growth and regeneration depending on subcellular localization and phosphorylation. ..
  12. Witherow D, Tovey S, Wang Q, Willars G, Slepak V. G beta 5.RGS7 inhibits G alpha q-mediated signaling via a direct protein-protein interaction. J Biol Chem. 2003;278:21307-13 pubmed
    ..Gbeta5 complex and cyan fluorescence protein-tagged Galphaq, indicating a direct interaction between these molecules. ..
  13. Hatzistergos K, Quevedo H, Oskouei B, Hu Q, Feigenbaum G, Margitich I, et al. Bone marrow mesenchymal stem cells stimulate cardiac stem cell proliferation and differentiation. Circ Res. 2010;107:913-22 pubmed publisher
    ..MSCs stimulate host CSCs, a new mechanism of action underlying successful cell-based therapeutics. ..
  14. Levay A, Peacock J, Lu Y, Koch M, Hinton R, Kadler K, et al. Scleraxis is required for cell lineage differentiation and extracellular matrix remodeling during murine heart valve formation in vivo. Circ Res. 2008;103:948-56 pubmed publisher
    ..Collectively, our studies have identified an in vivo requirement for scx during valvulogenesis and demonstrate its role in cell lineage differentiation and matrix distribution in remodeling valve structures. ..
  15. Kim J, Roberts C, Maruyama Y, Berg S, Roper S, Chaudhari N. Faithful expression of GFP from the PLCbeta2 promoter in a functional class of taste receptor cells. Chem Senses. 2006;31:213-9 pubmed
    ..These PLCbeta2 promoter-GFP transgenic lines promise to be useful for studying taste transduction, sensory signal processing, and taste bud development. ..
  16. Landin A, Kim J, Chaudhari N. Liposome-mediated transfection of mature taste cells. J Neurobiol. 2005;65:12-21 pubmed
    ..These studies should permit ex vivo strategies for studying development and cellular function in taste cells. ..
  17. Nair K, Hanson S, Mendez A, Gurevich E, Kennedy M, Shestopalov V, et al. Light-dependent redistribution of arrestin in vertebrate rods is an energy-independent process governed by protein-protein interactions. Neuron. 2005;46:555-67 pubmed
    ..These results indicate that the distribution of arrestin in rods is controlled by its dynamic interactions with rhodopsin in the OS and microtubules in the IS and that its movement occurs by simple diffusion...
  18. Enninga J, Levay A, Fontoura B. Sec13 shuttles between the nucleus and the cytoplasm and stably interacts with Nup96 at the nuclear pore complex. Mol Cell Biol. 2003;23:7271-84 pubmed
    ..These results indicate that Sec13 stably interacts with Nup96 at the NPC during interphase and that the shuttling of Sec13 between the nucleus and the cytoplasm may couple and regulate functions between these two compartments. ..
  19. Koilkonda R, Chou T, Porciatti V, Hauswirth W, Guy J. Induction of rapid and highly efficient expression of the human ND4 complex I subunit in the mouse visual system by self-complementary adeno-associated virus. Arch Ophthalmol. 2010;128:876-83 pubmed publisher
  20. Dvoryanchikov G, Sinclair M, Perea Martinez I, Wang T, Chaudhari N. Inward rectifier channel, ROMK, is localized to the apical tips of glial-like cells in mouse taste buds. J Comp Neurol. 2009;517:1-14 pubmed publisher
    ..We propose that in taste buds, ROMK in type I/glial-like cells may serve a homeostatic function, excreting excess K(+) through the apical pore, and allowing excitable taste cells to maintain a hyperpolarized resting membrane potential...
  21. Collette J, Chi R, Boettner D, Fernandez Golbano I, Plemel R, Merz A, et al. Clathrin functions in the absence of the terminal domain binding site for adaptor-associated clathrin-box motifs. Mol Biol Cell. 2009;20:3401-13 pubmed publisher
    ..These data suggest that additional or alternative binding sites exist on the TD propeller to help facilitate the recruitment of clathrin to sites of vesicle formation. ..
  22. Solans A, Zambrano A, Rodriguez M, Barrientos A. Cytotoxicity of a mutant huntingtin fragment in yeast involves early alterations in mitochondrial OXPHOS complexes II and III. Hum Mol Genet. 2006;15:3063-81 pubmed
    ..Our yeast model represents a very useful paradigm to study mitochondrial physiology alterations in the pathogenic mechanism of HD. ..
  23. Marin Castano M, Striker G, Alcazar O, Catanuto P, Espinosa Heidmann D, Cousins S. Repetitive nonlethal oxidant injury to retinal pigment epithelium decreased extracellular matrix turnover in vitro and induced sub-RPE deposits in vivo. Invest Ophthalmol Vis Sci. 2006;47:4098-112 pubmed
    ..This study suggests that HQ may be another type of oxidant that causes injury to the RPE and may explain the association between environmental oxidants and early AMD. ..
  24. Hunker C, Galvis A, Kruk I, Giambini H, Veisaga M, Barbieri M. Rab5-activating protein 6, a novel endosomal protein with a role in endocytosis. Biochem Biophys Res Commun. 2006;340:967-75 pubmed
    ..This study is the first to show that RAP6 is a novel regulator of endocytosis that exhibits GEF activity specific for Rab5 and GAP activity specific for Ras. ..
  25. Panickar K, Nonner D, Barrett J. Overexpression of Bcl-xl protects septal neurons from prolonged hypoglycemia and from acute ischemia-like stress. Neuroscience. 2005;135:73-80 pubmed
    ..Bcl-xl's protective effects may require an intact electron transport chain. ..
  26. Mustafi S, Rivero N, Olson J, Stahl P, Barbieri M. Regulation of Rab5 function during phagocytosis of live Pseudomonas aeruginosa in macrophages. Infect Immun. 2013;81:2426-36 pubmed publisher
    ..aeruginosa. These studies provide evidence that live P. aeruginosa cells are able to influence their rate of phagocytosis in macrophages by directly regulating activation of Rab5. ..
  27. Li W. Synergistic antibody induction by antigen-CD40 ligand fusion protein as improved immunogen. Immunology. 2005;115:215-22 pubmed
    ..These results suggest that GFP-CD40LT fusion protein induces a GFP-specific B-cell activation and antibody response in an antigen-guided fashion. The potential application of this novel strategy in vaccine development is discussed. ..