Experts and Doctors on drosophila in University Park, Florida, United States

Summary

Locale: University Park, Florida, United States
Topic: drosophila

Top Publications

  1. Zhang Y, Lai Z. Mob as tumor suppressor is regulated by bantam microRNA through a feedback loop for tissue growth control. Biochem Biophys Res Commun. 2013;439:438-42 pubmed publisher
    ..Our data support a model that bantam up-regulates Mats expression through an unidentified factor that may control Mats stability. ..
  2. Stone M, Rao K, Gheres K, Kim S, Tao J, La Rochelle C, et al. Normal spastin gene dosage is specifically required for axon regeneration. Cell Rep. 2012;2:1340-50 pubmed publisher
    ..Other types of axon and dendrite outgrowth, including regrowth of dendrites after pruning, were normal in heterozygotes. We conclude that regenerative axon growth is uniquely sensitive to spastin gene dosage. ..
  3. Gilbert S, Moyer M, Johnson K. Alternating site mechanism of the kinesin ATPase. Biochemistry. 1998;37:792-9 pubmed
  4. Russo C, Takezaki N, Nei M. Molecular phylogeny and divergence times of drosophilid species. Mol Biol Evol. 1995;12:391-404 pubmed publisher
    ..melanogaster groups apparently diverged about 25 Mya. Many of the D. repleta group species studied here have two functional Adh genes (Adh-1 and Adh-2), and these duplicated genes can be explained by two duplication events...
  5. Nozawa M, Miura S, Nei M. Origins and evolution of microRNA genes in Drosophila species. Genome Biol Evol. 2010;2:180-9 pubmed publisher
    ..Therefore, once miR genes acquired their functions, they appear to have evolved very slowly, maintaining essentially the same structures for a long time. ..
  6. Gilmour D, Fan R. Detecting transcriptionally engaged RNA polymerase in eukaryotic cells with permanganate genomic footprinting. Methods. 2009;48:368-74 pubmed publisher
    ..The method should prove useful in assessing if promoter bound Pol II has engaged in transcription and for investigating the establishment and regulation of transcriptionally engaged Pol II. ..
  7. Clark A, Wang L. Molecular population genetics of Drosophila immune system genes. Genetics. 1997;147:713-24 pubmed
    ..Patterns of interpopulation and interspecific differentiation also reveal differences among the genes in evolutionary forces...
  8. Purnell B, Emanuel P, Gilmour D. TFIID sequence recognition of the initiator and sequences farther downstream in Drosophila class II genes. Genes Dev. 1994;8:830-42 pubmed
    ..Selection of this sequence demonstrates that sequence-specific interactions downstream of the TATA element contribute to the interaction of TFIID on a wide spectrum of promoters. ..
  9. Nguyen M, Stone M, Rolls M. Microtubules are organized independently of the centrosome in Drosophila neurons. Neural Dev. 2011;6:38 pubmed publisher
    ..We conclude that the centrosome is not a major site of microtubule nucleation in Drosophila neurons, and is not required for maintenance of neuronal microtubule organization in these cells. ..

More Information

Publications56

  1. Chen L, Stone M, Tao J, Rolls M. Axon injury and stress trigger a microtubule-based neuroprotective pathway. Proc Natl Acad Sci U S A. 2012;109:11842-7 pubmed publisher
    ..We conclude that axon injury or stress triggers a microtubule-based neuroprotective pathway that stabilizes neurons against degeneration. ..
  2. Hill S, Parmar M, Gheres K, Guignet M, Huang Y, Jackson F, et al. Development of dendrite polarity in Drosophila neurons. Neural Dev. 2012;7:34 pubmed publisher
    ..Over time they mature to uniform microtubule polarity. Dendrite identity is established before the mature microtubule arrangement is attained, during the period of mixed microtubule polarity. ..
  3. Schaeffer S, Miller E. Estimates of gene flow in Drosophila pseudoobscura determined from nucleotide sequence analysis of the alcohol dehydrogenase region. Genetics. 1992;132:471-80 pubmed
    ..pseudoobscura. The nucleotide diversity data is consistent with direct and indirect measures of gene flow that show extensive dispersal between populations of D. pseudoobscura...
  4. Nei M, Zhang J. Molecular origin of species. Science. 1998;282:1428-9 pubmed
  5. Bour B, Chakravarti M, West J, Abmayr S. Drosophila SNS, a member of the immunoglobulin superfamily that is essential for myoblast fusion. Genes Dev. 2000;14:1498-511 pubmed
    ..To these ends, we demonstrate that the presence of SNS-expressing cells is absolutely dependent on Notch, and that expression of SNS does not require the myogenic regulatory protein MEF2. ..
  6. Price M, Lai Z. The yan gene is highly conserved in Drosophila and its expression suggests a complex role throughout development. Dev Genes Evol. 1999;209:207-17 pubmed
    ..Ovarian follicle cells also contain Yan protein. Conservation of the structure and expression patterns of yan genes strongly suggests that regulatory mechanisms for their expression are also conserved in these two species...
  7. Galletta B, Niu X, Erickson M, Abmayr S. Identification of a Drosophila homologue to vertebrate Crk by interaction with MBC. Gene. 1999;228:243-52 pubmed
    ..During embryogenesis, the spatial distribution of this transcript is relatively broad and appears to include all germ layers. Finally, dCrk is located on the fourth chromosome, approximately at cytological position 101F-102A. ..
  8. Steitz M, Wickenheisser J, Siegfried E. Overexpression of zeste white 3 blocks wingless signaling in the Drosophila embryonic midgut. Dev Biol. 1998;197:218-33 pubmed
  9. Toung Y, Hsieh T, Tu C. The Drosophila glutathione S-transferase 1-1 is encoded by an intronless gene at 87B. Biochem Biophys Res Commun. 1991;178:1205-11 pubmed
    ..We report that the glutathione S-transferase activity in Kco cells as determined by conjugation with 1-chloro-2,4-dinitrobenzene is elevated slightly to two-fold under heat shock. The implication of this finding is discussed. ..
  10. Tang A, Tu C. Pentobarbital-induced changes in Drosophila glutathione S-transferase D21 mRNA stability. J Biol Chem. 1995;270:13819-25 pubmed
    ..Thus, an increase in gstD21 mRNA stability by PB treatment is probably coupled to a regulatory effect at the translational level. ..
  11. Perdew G, Wiegand H, Vanden Heuvel J, Mitchell C, Singh S. A 50 kilodalton protein associated with raf and pp60(v-src) protein kinases is a mammalian homolog of the cell cycle control protein cdc37. Biochemistry. 1997;36:3600-7 pubmed
    ..These results link studies demonstrating complexation of certain kinases with hsp90/p50 in mammalian cells and a number of reports in yeast and Drosophila, demonstrating the importance of cdc37 in cell cycle and kinase function. ..
  12. Li J, Gilmour D. Distinct mechanisms of transcriptional pausing orchestrated by GAGA factor and M1BP, a novel transcription factor. EMBO J. 2013;32:1829-41 pubmed publisher
    ..In contrast to M1BP and GAF genes, a significant portion of TATA box genes appear to be controlled at preinitiation complex formation. ..
  13. Moyer M, Gilbert S, Johnson K. Purification and characterization of two monomeric kinesin constructs. Biochemistry. 1996;35:6321-9 pubmed
  14. Ahn Y, Zou J, Mitchell P. Segment-specific regulation of the Drosophila AP-2 gene during leg and antennal development. Dev Biol. 2011;355:336-48 pubmed publisher
  15. Thomas G, Zarnescu D, Juedes A, Bales M, Londergan A, Korte C, et al. Drosophila betaHeavy-spectrin is essential for development and contributes to specific cell fates in the eye. Development. 1998;125:2125-34 pubmed
  16. Phillips M, Thomas G. Brush border spectrin is required for early endosome recycling in Drosophila. J Cell Sci. 2006;119:1361-70 pubmed
    ..These data are consistent with the location of spectrin in the terminal web, and suggest that this molecule is required for correct sorting decisions at the early endosome. ..
  17. Lai Z, Wei X, Shimizu T, Ramos E, Rohrbaugh M, Nikolaidis N, et al. Control of cell proliferation and apoptosis by mob as tumor suppressor, mats. Cell. 2005;120:675-85 pubmed
    ..A human Mats ortholog (Mats1) can rescue the lethality associated with loss of Mats function in Drosophila. As Mats1 is mutated in human tumors, Mats-mediated growth inhibition and tumor suppression is likely conserved in humans. ..
  18. Toung Y, Hsieh T, Tu C. Drosophila glutathione S-transferase 1-1 shares a region of sequence homology with the maize glutathione S-transferase III. Proc Natl Acad Sci U S A. 1990;87:31-5 pubmed
    ..Drosophila glutathione S-transferase 1-1 had no obvious homology to any mammalian or parasitic glutathione S-transferases. The gene was found to be a member of a multigene family. ..
  19. Tjota M, Lee S, Wu J, Williams J, Khanna M, Thomas G. Annexin B9 binds to ?(H)-spectrin and is required for multivesicular body function in Drosophila. J Cell Sci. 2011;124:2914-26 pubmed publisher
    ..These results indicate that AnxB9 and ?(H) are intimately involved in endosomal trafficking to the MVB and play a role in maintaining high-fidelity segregation of the apical and lateral domains. ..
  20. Lee H, Tu C. Drosophila glutathione S-transferase D27: functional analysis of two consecutive tyrosines near the N-terminus. Biochem Biophys Res Commun. 1995;209:327-34 pubmed
    ..Results from in vitro biochemical analyses were confirmed by the in vivo activity-based CDNB growth inhibition analyses. Our results clearly indicate that the Drosophila GST D isozymes are different from any of the known mammalian GSTs. ..
  21. Nguyen D, Rohrbaugh M, Lai Z. The Drosophila homolog of Onecut homeodomain proteins is a neural-specific transcriptional activator with a potential role in regulating neural differentiation. Mech Dev. 2000;97:57-72 pubmed
    ..In support of this notion, overexpression of a putative dominant negative form of D-Onecut during eye development does not affect early cell fate specification, but severely affects photoreceptor differentiation. ..
  22. Benjamin L, Gilmour D. Nucleosomes are not necessary for promoter-proximal pausing in vitro on the Drosophila hsp70 promoter. Nucleic Acids Res. 1998;26:1051-5 pubmed
    ..Our results indicate that a downstream nucleosome is not a universal requirement for stably pausing RNA polymerase in the promoter-proximal region. ..
  23. Gilbert S, Webb M, Brune M, Johnson K. Pathway of processive ATP hydrolysis by kinesin. Nature. 1995;373:671-6 pubmed
    ..These results provide an explanation for the motility differences between skeletal myosin and kinesin. ..
  24. Schaeffer S, Miller E. Molecular population genetics of an electrophoretically monomorphic protein in the alcohol dehydrogenase region of Drosophila pseudoobscura. Genetics. 1992;132:163-78 pubmed
    ..The neutral mutation parameter for synonymous sites is heterogeneous between domains of the Adh region. These data indicate that selective constrains on synonymous sites can vary between functional domains...
  25. Clark A, Whittam T. Sequencing errors and molecular evolutionary analysis. Mol Biol Evol. 1992;9:744-52 pubmed
    ..A scan of GenBank for corrections of previous errors reveals that sequencing errors are highly nonrandom. ..
  26. Balczarek K, Lai Z, Kumar S. Evolution of functional diversification of the paired box (Pax) DNA-binding domains. Mol Biol Evol. 1997;14:829-42 pubmed
    ..Implications of such fixed amino acid differences in potentially generating sequence recognition specificities are discussed in the context of some recent experimental findings. ..
  27. Missra A, Gilmour D. Interactions between DSIF (DRB sensitivity inducing factor), NELF (negative elongation factor), and the Drosophila RNA polymerase II transcription elongation complex. Proc Natl Acad Sci U S A. 2010;107:11301-6 pubmed publisher
    ..This raises the possibility that RNA binding by NELF is not necessary in promoter-proximal pausing. ..
  28. Zou B, Yan H, Kawasaki F, Ordway R. MAP1 structural organization in Drosophila: in vivo analysis of FUTSCH reveals heavy- and light-chain subunits generated by proteolytic processing at a conserved cleavage site. Biochem J. 2008;414:63-71 pubmed publisher
  29. Clark A, Wang L. Epistasis in measured genotypes: Drosophila P-element insertions. Genetics. 1997;147:157-63 pubmed
    ..Measured genotypes have been used in other contexts to quantify epistatic effects on phenotypic expression, and these results are also briefly reviewed. ..
  30. Leicht B, Lyckegaard E, Benedict C, Clark A. Conservation of alternative splicing and genomic organization of the myosin alkali light-chain (Mlc1) gene among Drosophila species. Mol Biol Evol. 1993;10:769-90 pubmed
    ..Our results suggest that evolutionary constraints are acting on both the coding and noncoding sequences of the Mlc1 gene to maintain proper expression and function of the two MLC1 polypeptides. ..
  31. Takezaki N, Rzhetsky A, Nei M. Phylogenetic test of the molecular clock and linearized trees. Mol Biol Evol. 1995;12:823-33 pubmed publisher
    ..A linearized tree will then be constructed for a given topology for the remaining sequences under the assumption of rate constancy. We have used these methods to analyze hominoid mitochondrial DNA and drosophilid Adh gene sequences...
  32. Schaeffer S, Miller E. Nucleotide sequence analysis of Adh genes estimates the time of geographic isolation of the Bogota population of Drosophila pseudoobscura. Proc Natl Acad Sci U S A. 1991;88:6097-101 pubmed
    ..This divergence time suggests that D. pseudoobscura extended its range from North America to South America in a period of Pleistocene glaciation, when habitat suitable for the species presumably existed in lowland Central America...
  33. Nozawa M, Nei M. Evolutionary dynamics of olfactory receptor genes in Drosophila species. Proc Natl Acad Sci U S A. 2007;104:7122-7 pubmed
    ..The difference in evolutionary pattern between Drosophila and mammals can be explained partly by the differences of gene expression mechanisms and partly by the environmental and behavioral differences. ..
  34. Schaeffer S, Anderson W. Mechanisms of genetic exchange within the chromosomal inversions of Drosophila pseudoobscura. Genetics. 2005;171:1729-39 pubmed
    ..At least one case where selection rather than proximity to an inversion breakpoint is responsible for reduction in polymorphism was identified. ..
  35. Wu Y, Kawasaki F, Ordway R. Properties of short-term synaptic depression at larval neuromuscular synapses in wild-type and temperature-sensitive paralytic mutants of Drosophila. J Neurophysiol. 2005;93:2396-405 pubmed
  36. Schaeffer S, Goetting Minesky M, Kovacevic M, Peoples J, Graybill J, Miller J, et al. Evolutionary genomics of inversions in Drosophila pseudoobscura: evidence for epistasis. Proc Natl Acad Sci U S A. 2003;100:8319-24 pubmed
    ..pseudoobscura have emerged as suppressors of recombination to maintain positive epistatic relationships among loci within gene arrangements that developed as the species adapted to a heterogeneous environment. ..
  37. Neely K, Workman J. The complexity of chromatin remodeling and its links to cancer. Biochim Biophys Acta. 2002;1603:19-29 pubmed
    ..Their subunit composition and brief overview of their functional properties will be discussed. Also, current links between human chromatin-remodeling complexes and cell growth and cancer will be presented. ..
  38. Bour B, O Brien M, Lockwood W, Goldstein E, Bodmer R, Taghert P, et al. Drosophila MEF2, a transcription factor that is essential for myogenesis. Genes Dev. 1995;9:730-41 pubmed
    ..These results indicate that MEF2 is required for later aspects of differentiation of the three major types of musculature, which include body wall muscles, gut musculature, and the heart, in the Drosophila embryo. ..
  39. Lai Z, Fetchko M, Li Y. Repression of Drosophila photoreceptor cell fate through cooperative action of two transcriptional repressors Yan and Tramtrack. Genetics. 1997;147:1131-7 pubmed
    ..Reduction of either yan or ttk activity suppresses eye phenotypes of the kinase suppressor of ras (ksr) gene mutation, which is consistent with the involvement of yan and ttk in the Ras/MAPK pathway. ..
  40. Achary B, Campbell K, Co I, Gilmour D. RNAi screen in Drosophila larvae identifies histone deacetylase 3 as a positive regulator of the hsp70 heat shock gene expression during heat shock. Biochim Biophys Acta. 2014;1839:355-63 pubmed publisher
    ..Additionally, we show that HDAC3 and SMRTER contribute to hsp70 gene expression at a step subsequent to HSF-mediated activation and release of the paused Pol II that resides at the promoter prior to heat shock induction. ..
  41. Ghosh S, Missra A, Gilmour D. Negative elongation factor accelerates the rate at which heat shock genes are shut off by facilitating dissociation of heat shock factor. Mol Cell Biol. 2011;31:4232-43 pubmed publisher
    ..Activators are typically viewed as controlling Pol II. Our results reveal a possible reciprocal relationship in which paused Pol II influences the activator. ..
  42. Moyer M, Gilbert S, Johnson K. Pathway of ATP hydrolysis by monomeric and dimeric kinesin. Biochemistry. 1998;37:800-13 pubmed
  43. Meisel R. Repeat mediated gene duplication in the Drosophila pseudoobscura genome. Gene. 2009;438:1-7 pubmed publisher
    ..This suggests that the molecular mechanisms responsible for chromosomal rearrangements and some duplicated genes have overlapping processes. ..
  44. Schaeffer S, Miller E. Estimates of linkage disequilibrium and the recombination parameter determined from segregating nucleotide sites in the alcohol dehydrogenase region of Drosophila pseudoobscura. Genetics. 1993;135:541-52 pubmed
    ..The most likely explanation for the two clusters of linkage disequilibria is epistatic selection between sites in the cluster to maintain pre-mRNA secondary structure...
  45. Clark A, Leicht B, Muse S. Length variation and secondary structure of introns in the Mlc1 gene in six species of Drosophila. Mol Biol Evol. 1996;13:471-82 pubmed
    ..Relative rate tests also suggest a retardation in the rate of Mlc1 sequence evolution in the simulans clade. ..
  46. Schaeffer S. Molecular population genetics of sequence length diversity in the Adh region of Drosophila pseudoobscura. Genet Res. 2002;80:163-75 pubmed
    ..Genome rearrangement and size-dependent intron evolution are proposed as mechanisms that limit runaway intron expansion. ..
  47. Tang A, Tu C. Biochemical characterization of Drosophila glutathione S-transferases D1 and D21. J Biol Chem. 1994;269:27876-84 pubmed
    ..This is the first definitive demonstration that DDT dehydrochlorinase activity is an intrinsic property of a Drosophila GST. Our results suggest that GST D1 may play a role in DDT metabolism in Drosophila. ..