Experts and Doctors on acyltransferases in University Park, Florida, United States


Locale: University Park, Florida, United States
Topic: acyltransferases

Top Publications

  1. Inglese J, Smith J, Benkovic S. Active-site mapping and site-specific mutagenesis of glycinamide ribonucleotide transformylase from Escherichia coli. Biochemistry. 1990;29:6678-87 pubmed
    ..These results imply that Asp 144 may function as a general base within the catalytic center of the transformylase and is in close proximity to His 119 in the folded protein. ..
  2. Marolewski A, Smith J, Benkovic S. Cloning and characterization of a new purine biosynthetic enzyme: a non-folate glycinamide ribonucleotide transformylase from E. coli. Biochemistry. 1994;33:2531-7 pubmed
    ..The purT GAR transformylase is the first unknown de novo purine biosynthetic enzyme to be discovered in the last 30 years and represents another step forward in understanding cellular control of purine levels. ..
  3. Guo L, Phillips A, Arteca R. Amino acid N-malonyltransferases from mung beans. Action on 1-aminocyclopropane-1-carboxylic acid and D-phenylalanine. J Biol Chem. 1993;268:25389-94 pubmed
    ..The enzyme was also inhibited uncompetitively at high concentrations of malonyl-CoA. We propose that the enzyme follows an Ordered Bi-Bi reaction pathway, with the amino acid substrate being bound initially...
  4. Nixon A, Warren M, Benkovic S. Assembly of an active enzyme by the linkage of two protein modules. Proc Natl Acad Sci U S A. 1997;94:1069-73 pubmed
    ..The specific activity of two hybrid proteins was within 100- to 1000-fold of the native purN GAR transformylase validating the approach of constructing an enzyme active site from functional parts of others. ..
  5. Shimada T, Ross A, Muccio D, Brouillette W, Shealy Y. Regulation of hepatic lecithin:retinol acyltransferase activity by retinoic acid receptor-selective retinoids. Arch Biochem Biophys. 1997;344:220-7 pubmed
    ..920). These data implicate the RARs in the induction of hepatic LRAT and suggest a predominant role for RAR-alpha-active ligands. ..
  6. Zolfaghari R, Wang Y, Chen Q, Sancher A, Ross A. Cloning and molecular expression analysis of large and small lecithin:retinol acyltransferase mRNAs in the liver and other tissues of adult rats. Biochem J. 2002;368:621-31 pubmed
    ..The differential usage of these polyadenylation signals can explain the presence of multiple LRAT mRNA transcripts which are expressed in different tissue-specific patterns. ..
  7. Ross A, Zolfaghari R. Regulation of hepatic retinol metabolism: perspectives from studies on vitamin A status. J Nutr. 2004;134:269S-275S pubmed
    ..Moreover, retinoid metabolism in the liver is likely to be closely integrated with that in peripheral tissues through the rapid interorgan transfer and recycling of retinoids, affecting the whole-body economy of vitamin A. ..
  8. Nesbitt N, Baleanu Gogonea C, Cicchillo R, Goodson K, Iwig D, Broadwater J, et al. Expression, purification, and physical characterization of Escherichia coli lipoyl(octanoyl)transferase. Protein Expr Purif. 2005;39:269-82 pubmed
    ..The specific activity of the purified protein is 0.541 U mg(-1), indicating a turnover number of approximately 0.2 s(-1), and the apparent Km values for octanoyl-ACP and apo-H protein are 10.2+/-4.4 and 13.2+/-2.9 microM, respectively. ..
  9. Maresca J, Bryant D. Two genes encoding new carotenoid-modifying enzymes in the green sulfur bacterium Chlorobium tepidum. J Bacteriol. 2006;188:6217-23 pubmed
    ..The other bacteria in which these genes are found are not closely related to green sulfur bacteria or to one another. This suggests that the ability to synthesize modified carotenoids has been a frequently transferred trait...

More Information


  1. Wu L, Ross A. Acidic retinoids synergize with vitamin A to enhance retinol uptake and STRA6, LRAT, and CYP26B1 expression in neonatal lung. J Lipid Res. 2010;51:378-87 pubmed publisher
    ..However, the pronounced and prolonged induction of CYP26B1 by Am580 may counteract lung RE accumulation after the absorption process is completed. ..
  2. Inglese J, Johnson D, Shiau A, Smith J, Benkovic S. Subcloning, characterization, and affinity labeling of Escherichia coli glycinamide ribonucleotide transformylase. Biochemistry. 1990;29:1436-43 pubmed
    ..The ionization state of the cofactor analogue in the enzyme-cofactor complex appears to require the dissociation of the proton at N3 of the pyrimidine within the complex. ..
  3. Zolfaghari R, Ross A. Lecithin:retinol acyltransferase from mouse and rat liver. CDNA cloning and liver-specific regulation by dietary vitamin a and retinoic acid. J Lipid Res. 2000;41:2024-34 pubmed
  4. Zolfaghari R, Ross A. Cloning, gene organization and identification of an alternative splicing process in lecithin:retinol acyltransferase cDNA from human liver. Gene. 2004;341:181-8 pubmed
  5. Estes K, Kalamegham R, Hanna Rose W. Membrane localization of the NlpC/P60 family protein EGL-26 correlates with regulation of vulval cell morphogenesis in Caenorhabditis elegans. Dev Biol. 2007;308:196-205 pubmed
    ..Additional mutagenesis of this residue suggests that EGL-26 attains its membrane localization via a mechanism distinct from that of LRAT. ..
  6. Zolfaghari R, Ross A. An essential set of basic DNA response elements is required for receptor-dependent transcription of the lecithin:retinol acyltransferase (Lrat) gene. Arch Biochem Biophys. 2009;489:1-9 pubmed publisher
    ..Nuclear extracts from RA-treated cells exhibited enhanced binding. Therefore, this proximal region together with basal transcription factors may be sufficient to drive Lrat expression. ..