Experts and Doctors on saccharomyces cerevisiae proteins in New York, United States

Summary

Locale: New York, United States
Topic: saccharomyces cerevisiae proteins

Top Publications

  1. Cullmann G, Fien K, Kobayashi R, Stillman B. Characterization of the five replication factor C genes of Saccharomyces cerevisiae. Mol Cell Biol. 1995;15:4661-71 pubmed
    ..CDC44/RFC1 is known to interact genetically with the gene encoding proliferating cell nuclear antigen, confirming previous biochemical evidence of their functional interaction in DNA replication. ..
  2. Su S, Mitchell A. Molecular characterization of the yeast meiotic regulatory gene RIM1. Nucleic Acids Res. 1993;21:3789-97 pubmed
    ..These findings suggest that RIM1 acts independently of MCK1 and IME4 to stimulate IME1 expression. ..
  3. Cross F, Blake C. The yeast Cln3 protein is an unstable activator of Cdc28. Mol Cell Biol. 1993;13:3266-71 pubmed
    ..These and other results strongly support the idea that Cln proteins function to activate Cdc28 at START. ..
  4. Doseff A, Arndt K. LAS1 is an essential nuclear protein involved in cell morphogenesis and cell surface growth. Genetics. 1995;141:857-71 pubmed
    ..The nuclear localization of LAS1 suggests a role of LAS1 for regulating bud formation and morphogenesis via the expression of components that function directly in these processes. ..
  5. Schwer B, Mao X, Shuman S. Accelerated mRNA decay in conditional mutants of yeast mRNA capping enzyme. Nucleic Acids Res. 1998;26:2050-7 pubmed
    ..These findings provide genetic evidence for the long-held idea that the cap guanylate is critical for mRNA stability. The deadenylation-decapping-degradation pathway appears to be short-circuited when Ceg1 is inactivated. ..
  6. Liu H, Su Y, Becker E, Treisman J, Skolnik E. A Drosophila TNF-receptor-associated factor (TRAF) binds the ste20 kinase Misshapen and activates Jun kinase. Curr Biol. 1999;9:101-4 pubmed
    ..We have extended some of these observations to the mammalian homolog of Msn, Nck-interacting kinase (NIK), suggesting that TRAFs also play a critical role in regulating Ste20 kinases in mammals. ..
  7. Gu W, Malik S, Ito M, Yuan C, Fondell J, Zhang X, et al. A novel human SRB/MED-containing cofactor complex, SMCC, involved in transcription regulation. Mol Cell. 1999;3:97-108 pubmed
    ..These findings demonstrate both positive and negative functional capabilities for the human complex, emphasize novel (CTD-independent) regulatory mechanisms, and link the complex to other human coactivator complexes. ..
  8. Malathi K, Xiao Y, Mitchell A. Catalytic roles of yeast GSK3beta/shaggy homolog Rim11p in meiotic activation. Genetics. 1999;153:1145-52 pubmed
    ..Therefore, Ime1p and Rim11p have two genetically separable functions in the sporulation program. However, catalytic activity of Rim11p is required for sporulation in the presence or absence of Ume6p. ..
  9. Glynn J, Lustig R, Berlin A, Chang F. Role of bud6p and tea1p in the interaction between actin and microtubules for the establishment of cell polarity in fission yeast. Curr Biol. 2001;11:836-45 pubmed
    ..To our knowledge, bud6p is the first protein shown to interact physically with tea1p. These studies delineate a pathway for how microtubule plus ends function to polarize the actin cytoskeleton through actin-associated polarity factors. ..

More Information

Publications412 found, 100 shown here

  1. Martini E, Keeney S, Osley M. A role for histone H2B during repair of UV-induced DNA damage in Saccharomyces cerevisiae. Genetics. 2002;160:1375-87 pubmed
    ..When combined with a ubc13Delta mutation, which is also epistatic with rad5Delta, the htb1-3 mutation enhanced UV-induced cell killing. These results suggest that histone H2B acts in a novel RAD5-dependent branch of PRR. ..
  2. Kniewel R, Buglino J, Shen V, Chadha T, Beckwith A, Lima C. Structural analysis of Saccharomyces cerevisiae myo-inositol phosphate synthase. J Struct Funct Genomics. 2002;2:129-34 pubmed
    ..The structure further reveals extensive interactions involved in tetrameric assembly of the enzyme complex. ..
  3. Liu L, Query C, Konarska M. Opposing classes of prp8 alleles modulate the transition between the catalytic steps of pre-mRNA splicing. Nat Struct Mol Biol. 2007;14:519-26 pubmed
    ..Modulation of these events alters splice-site selection and splicing fidelity. ..
  4. Pagant S, Brovman E, Halliday J, Miller E. Mapping of interdomain interfaces required for the functional architecture of Yor1p, a eukaryotic ATP-binding cassette (ABC) transporter. J Biol Chem. 2008;283:26444-51 pubmed publisher
  5. Seo H, Ma Y, Debler E, Wacker D, Kutik S, Blobel G, et al. Structural and functional analysis of Nup120 suggests ring formation of the Nup84 complex. Proc Natl Acad Sci U S A. 2009;106:14281-6 pubmed publisher
    ..These results illuminate important roles of terminal unstructured segments in nucleoporins for the architecture, function, and assembly of the NPC. ..
  6. Smith D, Whitehouse I. Intrinsic coupling of lagging-strand synthesis to chromatin assembly. Nature. 2012;483:434-8 pubmed publisher
    ..Our studies represent the first high-resolution analysis--to our knowledge--of eukaryotic Okazaki fragments in vivo, and reveal the interconnection between lagging-strand synthesis and chromatin assembly. ..
  7. Statnikov A, Henaff M, Lytkin N, Aliferis C. New methods for separating causes from effects in genomics data. BMC Genomics. 2012;13 Suppl 8:S22 pubmed publisher
  8. Das J, Vo T, Wei X, Mellor J, TONG V, Degatano A, et al. Cross-species protein interactome mapping reveals species-specific wiring of stress response pathways. Sci Signal. 2013;6:ra38 pubmed publisher
  9. Baker L, Ueberheide B, Dewell S, Chait B, Zheng D, Allis C. The yeast Snt2 protein coordinates the transcriptional response to hydrogen peroxide-mediated oxidative stress. Mol Cell Biol. 2013;33:3735-48 pubmed publisher
    ..Our results establish a function for Snt2 in regulating transcription in response to oxidative stress and suggest Snt2 may also function in multiple stress pathways. ..
  10. Galdieri L, Chang J, Mehrotra S, Vancura A. Yeast phospholipase C is required for normal acetyl-CoA homeostasis and global histone acetylation. J Biol Chem. 2013;288:27986-98 pubmed publisher
    ..Together, our data indicate that Plc1p and InsPs are required for normal acetyl-CoA homeostasis, which, in turn, regulates global histone acetylation. ..
  11. Hang L, Lopez C, Liu X, Williams J, Chung I, Wei L, et al. Regulation of Ku-DNA association by Yku70 C-terminal tail and SUMO modification. J Biol Chem. 2014;289:10308-17 pubmed publisher
    ..These results suggest a model whereby Yku70 sumoylation upon DNA association strengthens Ku-DNA interaction to promote multiple functions of Ku. ..
  12. Ackerman S, Martin J, Tzagoloff A. Characterization of ATP11 and detection of the encoded protein in mitochondria of Saccharomyces cerevisiae. J Biol Chem. 1992;267:7386-94 pubmed
    ..A fraction eluted from the avidin column and enriched for the biotinated ATP11 protein also contains the alpha and beta subunits of F1-ATPase. ..
  13. Li B, Warner J. Mutation of the Rab6 homologue of Saccharomyces cerevisiae, YPT6, inhibits both early Golgi function and ribosome biosynthesis. J Biol Chem. 1996;271:16813-9 pubmed
  14. Pemberton L, Blobel G. Characterization of the Wtm proteins, a novel family of Saccharomyces cerevisiae transcriptional modulators with roles in meiotic regulation and silencing. Mol Cell Biol. 1997;17:4830-41 pubmed
    ..These data suggested that Wtm1p and Wtm2p are repressors and that Wtm3p has different effects on transcription at different loci. ..
  15. Lee P, Rao J, Fliss A, Yang E, Garrett S, Caplan A. The Cdc37 protein kinase-binding domain is sufficient for protein kinase activity and cell viability. J Cell Biol. 2002;159:1051-9 pubmed
    ..This may be a form of regulation by which cells restrict access to Cdc37 until it has passed through a triage involving other chaperones such as Hsp70 and Hsp90. ..
  16. Lin J, Duell K, Saracino M, Konopka J. Identification of residues that contribute to receptor activation through the analysis of compensatory mutations in the G protein-coupled alpha-factor receptor. Biochemistry. 2005;44:1278-87 pubmed
    ..These results suggest that transmembrane domain 7 plays a role in receptor activation in a wide range of G protein-coupled receptors from yeast to humans. ..
  17. Gehret A, Bajaj A, Naider F, Dumont M. Oligomerization of the yeast alpha-factor receptor: implications for dominant negative effects of mutant receptors. J Biol Chem. 2006;281:20698-714 pubmed
    ..Thus, signal transduction by oligomeric receptors appears to be a cooperative process requiring an interaction between functional monomers. ..
  18. Liu Q, Greimann J, Lima C. Reconstitution, activities, and structure of the eukaryotic RNA exosome. Cell. 2006;127:1223-37 pubmed
    ..To elucidate the architecture of a eukaryotic exosome, its conserved surfaces, and the structural basis for RNA decay, we report the X-ray structure determination for the 286 kDa nine-subunit human exosome at 3.35 A. ..
  19. Ron D. Targeting of mRNAs to their sites of unconventional splicing in the unfolded protein response. Mol Cell. 2009;34:133-4 pubmed publisher
    ..2009) are reports on the importance of mRNA localization for unconventional splicing in the unfolded protein response, but the two papers suggest very different mechanisms in mammals and yeast. ..
  20. Gothwal S, Patel N, Colletti M, Sasanuma H, Shinohara M, Hochwagen A, et al. The Double-Strand Break Landscape of Meiotic Chromosomes Is Shaped by the Paf1 Transcription Elongation Complex in Saccharomyces cerevisiae. Genetics. 2016;202:497-512 pubmed publisher
    ..These observations suggest that Rtf1, and by extension the Paf1C, modulate the genomic DSB landscape independently of H3K4 methylation. ..
  21. Medintz I, Jiang H, Michels C. The role of ubiquitin conjugation in glucose-induced proteolysis of Saccharomyces maltose permease. J Biol Chem. 1998;273:34454-62 pubmed
    ..Loss of these functions was not shown to effect glucose-induced proteolysis of maltose permease, but loss of Ubc1, -4, and -5 was found to inhibit maltose permease expression at the post-transcriptional level. ..
  22. Merker R, Klein H. hpr1Delta affects ribosomal DNA recombination and cell life span in Saccharomyces cerevisiae. Mol Cell Biol. 2002;22:421-9 pubmed
    ..The hpr1Delta mutant acts in a pathway distinct from previously described mutants that reduce life span. ..
  23. Yan Q, Lennarz W. Studies on the function of oligosaccharyl transferase subunits. Stt3p is directly involved in the glycosylation process. J Biol Chem. 2002;277:47692-700 pubmed
    ..From all of these results, we conclude that the sequence from residues 516 to 520, WWDYG in Stt3p, plays a central role in glycosylatable peptide recognition and/or the catalytic glycosylation process. ..
  24. Waldo J, Scherrer M. Production and initial characterization of Dad1p, a component of the Dam1-DASH kinetochore complex. PLoS ONE. 2008;3:e3888 pubmed publisher
  25. McIntosh K, Bhattacharya A, Willis I, Warner J. Eukaryotic cells producing ribosomes deficient in Rpl1 are hypersensitive to defects in the ubiquitin-proteasome system. PLoS ONE. 2011;6:e23579 pubmed publisher
    ..Interestingly, in spite of rendering the cells hypersensitive to the proteasome inhibitor MG132, shortage of Rpl1 partially suppresses the stress-invoked temporary repression of ribosome synthesis caused by MG132...
  26. Lo H, Kunz R, Chen X, Marullo A, Gygi S, Hollingsworth N. Cdc7-Dbf4 is a gene-specific regulator of meiotic transcription in yeast. Mol Cell Biol. 2012;32:541-57 pubmed publisher
    ..Analysis of various phosphorylation site mutants suggests that Cdc7 functions with both Cdk1 and the meiosis-specific kinase Ime2 to control this critical transition point during meiosis. ..
  27. Aitchison J, Blobel G, Rout M. Kap104p: a karyopherin involved in the nuclear transport of messenger RNA binding proteins. Science. 1996;274:624-7 pubmed
    ..This finding suggests that the major function of Kap104p lies in returning mRNA binding proteins to the nucleus after mRNA export. ..
  28. Vannier D, Balderes D, Shore D. Evidence that the transcriptional regulators SIN3 and RPD3, and a novel gene (SDS3) with similar functions, are involved in transcriptional silencing in S. cerevisiae. Genetics. 1996;144:1343-53 pubmed
    ..Together these results show that defects in certain general transcriptional modifiers can have a pronounced influence on position-effect gene silencing in yeast. Mechanisms for this increase in position effect are discussed. ..
  29. Brennwald P. Reversal of fortune. Do Rab GTPases act on the target membrane?. J Cell Biol. 2000;149:1-4 pubmed
  30. Lee J, Hurwitz J. Processive DNA helicase activity of the minichromosome maintenance proteins 4, 6, and 7 complex requires forked DNA structures. Proc Natl Acad Sci U S A. 2001;98:54-9 pubmed
    ..These results support the hypothesis that the Mcm4/6/7 complex can function as a replication helicase. ..
  31. Parlati F, Varlamov O, Paz K, McNew J, Hurtado D, Sollner T, et al. Distinct SNARE complexes mediating membrane fusion in Golgi transport based on combinatorial specificity. Proc Natl Acad Sci U S A. 2002;99:5424-9 pubmed
    ..6%. ..
  32. Boldogh I, Nowakowski D, Yang H, Chung H, Karmon S, Royes P, et al. A protein complex containing Mdm10p, Mdm12p, and Mmm1p links mitochondrial membranes and DNA to the cytoskeleton-based segregation machinery. Mol Biol Cell. 2003;14:4618-27 pubmed
  33. Langston L, Symington L. Gene targeting in yeast is initiated by two independent strand invasions. Proc Natl Acad Sci U S A. 2004;101:15392-7 pubmed
  34. Niranjan T, Guo X, Victor J, Lu A, Hirsch J. Kelch repeat protein interacts with the yeast Galpha subunit Gpa2p at a site that couples receptor binding to guanine nucleotide exchange. J Biol Chem. 2007;282:24231-8 pubmed
    ..The results suggest that binding of Gpa2p to Krh1p does not resemble the binding of Galpha subunits to either Gbeta subunits or effectors, but it instead represents a novel type of functional interaction. ..
  35. Tian G, Kober F, Lewandrowski U, Sickmann A, Lennarz W, Schindelin H. The catalytic activity of protein-disulfide isomerase requires a conformationally flexible molecule. J Biol Chem. 2008;283:33630-40 pubmed publisher
  36. Oeffinger M, Zenklusen D, Ferguson A, Wei K, El Hage A, Tollervey D, et al. Rrp17p is a eukaryotic exonuclease required for 5' end processing of Pre-60S ribosomal RNA. Mol Cell. 2009;36:768-81 pubmed publisher
    ..We show that Rrp17p binds late pre-60S ribosomes, accompanying them from the nucleolus to the nuclear periphery, and provide evidence for physical and functional links between late 60S subunit processing and export. ..
  37. Ziv N, Shuster B, Siegal M, Gresham D. Resolving the Complex Genetic Basis of Phenotypic Variation and Variability of Cellular Growth. Genetics. 2017;206:1645-1657 pubmed publisher
  38. Horowitz D, Kobayashi R, Krainer A. A new cyclophilin and the human homologues of yeast Prp3 and Prp4 form a complex associated with U4/U6 snRNPs. RNA. 1997;3:1374-87 pubmed
    ..The third protein in the complex is a new cyclophilin. Cyclophilins have been proposed to act as chaperones in a variety of cellular processes, and we discuss some possible roles of this U4/U6 snRNP-associated cyclophilin. ..
  39. Colby G, Wu M, Tzagoloff A. MTO1 codes for a mitochondrial protein required for respiration in paromomycin-resistant mutants of Saccharomyces cerevisiae. J Biol Chem. 1998;273:27945-52 pubmed
    ..To explain these phenotypic characteristics, we proposed that the Mto1p.Mss1p complex plays a role in optimizing mitochondrial protein synthesis in yeast, possibly by a proofreading mechanism. ..
  40. Neiman A, Katz L, Brennwald P. Identification of domains required for developmentally regulated SNARE function in Saccharomyces cerevisiae. Genetics. 2000;155:1643-55 pubmed
    ..Deletion studies indicate that activation and inhibition are separable functions of the Spo20p N terminus. Our results reveal an additional layer of regulation of the SNARE complex, which is necessary only in sporulating cells. ..
  41. Wasch R, Cross F. APC-dependent proteolysis of the mitotic cyclin Clb2 is essential for mitotic exit. Nature. 2002;418:556-62 pubmed
    ..Cdh1 and Sic1 may be required for further inactivation of Clb2-Cdk1, regulating cell size and the length of G1. ..
  42. Kee K, Protacio R, Arora C, Keeney S. Spatial organization and dynamics of the association of Rec102 and Rec104 with meiotic chromosomes. EMBO J. 2004;23:1815-24 pubmed
    ..These studies reveal unexpected behaviors for Rec102 and Rec104, and point to distinct roles and subcomplexes among the DSB proteins. ..
  43. Fung C, Fortin G, Peterson S, Symington L. The rad51-K191R ATPase-defective mutant is impaired for presynaptic filament formation. Mol Cell Biol. 2006;26:9544-54 pubmed
    ..We suggest the Rad51-K191R protein either forms an altered filament or is defective in turnover, resulting in a reduced pool of free protein available for DNA binding. ..
  44. Hardy C, Sussel L, Shore D. A RAP1-interacting protein involved in transcriptional silencing and telomere length regulation. Genes Dev. 1992;6:801-14 pubmed
    ..By use of the two-hybrid system, we have isolated a mutation in RIF1 that partially restores the interaction with rap1s mutant proteins. ..
  45. Field J, Vojtek A, Ballester R, Bolger G, Colicelli J, Ferguson K, et al. Cloning and characterization of CAP, the S. cerevisiae gene encoding the 70 kd adenylyl cyclase-associated protein. Cell. 1990;61:319-27 pubmed
  46. Kuchin S, Yeghiayan P, Carlson M. Cyclin-dependent protein kinase and cyclin homologs SSN3 and SSN8 contribute to transcriptional control in yeast. Proc Natl Acad Sci U S A. 1995;92:4006-10 pubmed
    ..Genetic analysis indicates that the SSN3-SSN8 complex contributes to transcriptional repression of diversely regulated genes and also affects induction of the GAL1 promoter. ..
  47. Bailly V, Lamb J, Sung P, Prakash S, Prakash L. Specific complex formation between yeast RAD6 and RAD18 proteins: a potential mechanism for targeting RAD6 ubiquitin-conjugating activity to DNA damage sites. Genes Dev. 1994;8:811-20 pubmed
    ..We also show that RAD6 forms separate complexes with RAD18 and with UBR1, and the extremely conserved amino terminus of RAD6 that is required for complex formation with UBR1 is dispensable for complex formation with RAD18. ..
  48. Lin E, Kozak C, Orlofsky A, Prystowsky M. The bcl-2 family member, Bcl2a1, maps to mouse chromosome 9 and human chromosome 15. Mamm Genome. 1997;8:293-4 pubmed
  49. Fang Y, Fliss A, Rao J, Caplan A. SBA1 encodes a yeast hsp90 cochaperone that is homologous to vertebrate p23 proteins. Mol Cell Biol. 1998;18:3727-34 pubmed
    ..In vivo analysis of known Hsp90 substrate proteins revealed that Sba1 loss of function had only a mild effect on the activity of the tyrosine kinase v-Src and steroid hormone receptors. ..
  50. Merker R, Klein H. Role of transcription in plasmid maintenance in the hpr1Delta mutant of Saccharomyces cerevisiae. Mol Cell Biol. 2002;22:8763-73 pubmed
    ..Models for the role of Hpr1p in mature mRNA formation and the cause of plasmid instability in the absence of the Hpr1 protein are discussed. ..
  51. Gundersen G, Bretscher A. Cell biology. Microtubule asymmetry. Science. 2003;300:2040-1 pubmed
  52. Feierbach B, Verde F, Chang F. Regulation of a formin complex by the microtubule plus end protein tea1p. J Cell Biol. 2004;165:697-707 pubmed
    ..Thus, these experiments begin to elucidate how microtubules contribute to the proper spatial regulation of actin assembly and polarized cell growth. ..
  53. Barrientos A, Zambrano A, Tzagoloff A. Mss51p and Cox14p jointly regulate mitochondrial Cox1p expression in Saccharomyces cerevisiae. EMBO J. 2004;23:3472-82 pubmed
    ..The release of Mss51p from the complex occurs at a downstream step in the assembly pathway, probably catalyzed by Shy1p. ..
  54. Schwartz T, Walczak R, Blobel G. Circular permutation as a tool to reduce surface entropy triggers crystallization of the signal recognition particle receptor beta subunit. Protein Sci. 2004;13:2814-8 pubmed
    ..The success of this method profits from prior knowledge of the domain fold, which is becoming increasingly common in today's postgenomic era. ..
  55. Gadura N, Robinson L, Michels C. Glc7-Reg1 phosphatase signals to Yck1,2 casein kinase 1 to regulate transport activity and glucose-induced inactivation of Saccharomyces maltose permease. Genetics. 2006;172:1427-39 pubmed
  56. Aronova A, Bacikova D, Crotti L, Horowitz D, Schwer B. Functional interactions between Prp8, Prp18, Slu7, and U5 snRNA during the second step of pre-mRNA splicing. RNA. 2007;13:1437-44 pubmed
  57. Debler E, Ma Y, Seo H, Hsia K, Noriega T, Blobel G, et al. A fence-like coat for the nuclear pore membrane. Mol Cell. 2008;32:815-26 pubmed publisher
    ..Shared architectural and mechanistic principles with the COPII coat indicate a common evolutionary origin and support the notion that the NPC coat represents another class of membrane coats. ..
  58. Boothroyd C, Dreesen O, Leonova T, Ly K, Figueiredo L, Cross G, et al. A yeast-endonuclease-generated DNA break induces antigenic switching in Trypanosoma brucei. Nature. 2009;459:278-81 pubmed publisher
  59. Ho C, Mazon G, Lam A, Symington L. Mus81 and Yen1 promote reciprocal exchange during mitotic recombination to maintain genome integrity in budding yeast. Mol Cell. 2010;40:988-1000 pubmed publisher
  60. Copic A, Latham C, Horlbeck M, D Arcangelo J, Miller E. ER cargo properties specify a requirement for COPII coat rigidity mediated by Sec13p. Science. 2012;335:1359-62 pubmed publisher
    ..Thus, Sec13p may rigidify the COPII cage and increase its membrane-bending capacity; this function could be bypassed when a bst mutation renders the membrane more deformable. ..
  61. Wang D, Mansisidor A, Prabhakar G, Hochwagen A. Condensin and Hmo1 Mediate a Starvation-Induced Transcriptional Position Effect within the Ribosomal DNA Array. Cell Rep. 2016;14:1010-1017 pubmed publisher
    ..Starvation-induced nucleolar contraction and differential desilencing of the outer rDNA repeats may provide a mechanism to activate rDNA-encoded RNAPII transcription units without causing general rDNA instability. ..
  62. Lesage P, Yang X, Carlson M. Yeast SNF1 protein kinase interacts with SIP4, a C6 zinc cluster transcriptional activator: a new role for SNF1 in the glucose response. Mol Cell Biol. 1996;16:1921-8 pubmed
    ..These findings suggest that the SNF1 kinase interacts with a transcriptional activator to modulate its activity and provide the first direct evidence for a role of SNF1 in activating transcription in response to glucose limitation. ..
  63. Couve A, Hirsch J. Loss of sustained Fus3p kinase activity and the G1 arrest response in cells expressing an inappropriate pheromone receptor. Mol Cell Biol. 1996;16:4478-85 pubmed
  64. Compagnone Post P, Osley M. Mutations in the SPT4, SPT5, and SPT6 genes alter transcription of a subset of histone genes in Saccharomyces cerevisiae. Genetics. 1996;143:1543-54 pubmed
    ..The results are consistent with the idea that the effects of the spt mutations on nucleosome assembly and/or stability activate repressors of HTA1 transcription. ..
  65. Wolf E, Vassilev A, Makino Y, Sali A, Nakatani Y, Burley S. Crystal structure of a GCN5-related N-acetyltransferase: Serratia marcescens aminoglycoside 3-N-acetyltransferase. Cell. 1998;94:439-49 pubmed
    ..Motif B contributes acidic residues to the binding site for the positively charged antibiotic substrate...
  66. Song W, Carlson M. Srb/mediator proteins interact functionally and physically with transcriptional repressor Sfl1. EMBO J. 1998;17:5757-65 pubmed
    ..We propose that Sfl1, when bound to its site, interacts with Srb/mediator proteins to inhibit transcription by RNA polymerase II holoenzyme. ..
  67. Lu A, Hirsch J. Cyclic AMP-independent regulation of protein kinase A substrate phosphorylation by Kelch repeat proteins. Eukaryot Cell. 2005;4:1794-800 pubmed
    ..These findings suggest that Gpb1p and Gpb2p mediate their effects on the cAMP/PKA signaling pathway either by inhibiting the activity of PKA in a cAMP-independent manner or by activating phosphatases that act on PKA substrates. ..
  68. Hedbacker K, Carlson M. Regulation of the nucleocytoplasmic distribution of Snf1-Gal83 protein kinase. Eukaryot Cell. 2006;5:1950-6 pubmed
    ..Finally, we characterize the effects of glucose phosphorylation on localization. These studies define roles for Snf1 and Gal83 in determining the nucleocytoplasmic distribution of Snf1-Gal83 protein kinase. ..
  69. Tanaka N, Aronova A, Schwer B. Ntr1 activates the Prp43 helicase to trigger release of lariat-intron from the spliceosome. Genes Dev. 2007;21:2312-25 pubmed
    ..Our results demonstrate for the first time that regulating the motor activity of a DEAH-box protein by an accessory factor is critical for mRNA splicing. ..
  70. Floer M, Bryant G, Ptashne M. HSP90/70 chaperones are required for rapid nucleosome removal upon induction of the GAL genes of yeast. Proc Natl Acad Sci U S A. 2008;105:2975-80 pubmed publisher
    ..Removal of promoter-bound nucleosomes is delayed in a chaperone mutant, and our findings suggest an involvement of HSP90 and HSP70 in this early step in gene induction. ..
  71. Mitchell A. A VAST staging area for regulatory proteins. Proc Natl Acad Sci U S A. 2008;105:7111-2 pubmed publisher
  72. Garbarino J, Padamsee M, Wilcox L, Oelkers P, D Ambrosio D, Ruggles K, et al. Sterol and diacylglycerol acyltransferase deficiency triggers fatty acid-mediated cell death. J Biol Chem. 2009;284:30994-1005 pubmed publisher
    ..In metazoans, many of these processes are implicated in insulin secretion thus linking lipotoxicity with early aspects of pancreatic beta-cell dysfunction, diabetes, and the metabolic syndrome. ..
  73. Ran F, Gadura N, Michels C. Hsp90 cochaperone Aha1 is a negative regulator of the Saccharomyces MAL activator and acts early in the chaperone activation pathway. J Biol Chem. 2010;285:13850-62 pubmed publisher
    ..These results suggest an interaction between Aha1 and residues near the C terminus of Mal63 thereby regulating Hsp90 association. A novel mechanism for the negative regulation of the MAL activator by Aha1 cochaperone is proposed. ..
  74. Powrie E, Zenklusen D, Singer R. A nucleoporin, Nup60p, affects the nuclear and cytoplasmic localization of ASH1 mRNA in S. cerevisiae. RNA. 2011;17:134-44 pubmed publisher
    ..These results demonstrate transcript specificity of a nuclear mRNA retention defect and identify a specific nucleoporin as a functional component of the localization pathway in budding yeast. ..
  75. Januszyk K, Liu Q, Lima C. Activities of human RRP6 and structure of the human RRP6 catalytic domain. RNA. 2011;17:1566-77 pubmed publisher
  76. Wilson M, Harreman M, Taschner M, Reid J, Walker J, Erdjument Bromage H, et al. Proteasome-mediated processing of Def1, a critical step in the cellular response to transcription stress. Cell. 2013;154:983-995 pubmed publisher
    ..Together, these results outline the multistep mechanism of Rpb1 polyubiquitylation triggered by transcription stress and uncover the key role played by Def1 as a facilitator of Elongin-Cullin ubiquitin ligase function. ..
  77. Pan Z, Chen M, Hurwitz J. The subunits of activator 1 (replication factor C) carry out multiple functions essential for proliferating-cell nuclear antigen-dependent DNA synthesis. Proc Natl Acad Sci U S A. 1993;90:6-10 pubmed
    ..These activities are essential for the DNA elongation reactions that lead to the synthesis of leading-strand DNA and the maturation of Okazaki fragments. ..
  78. Cross F, Levine K. Genetic analysis of the relationship between activation loop phosphorylation and cyclin binding in the activation of the Saccharomyces cerevisiae Cdc28p cyclin-dependent kinase. Genetics. 2000;154:1549-59 pubmed
    ..This conclusion was supported by analysis of suppressors of a mutation in the Cdk phosphothreonine-binding pocket created by cyclin binding. ..
  79. Oelkers P, Cromley D, Padamsee M, Billheimer J, Sturley S. The DGA1 gene determines a second triglyceride synthetic pathway in yeast. J Biol Chem. 2002;277:8877-81 pubmed
    ..Yeast strains deficient in both diacylglycerol and sterol esterification showed only a slight growth defect indicating that neutral lipid synthesis is dispensable under common laboratory conditions. ..
  80. Martin A, Schneider S, Schwer B. Prp43 is an essential RNA-dependent ATPase required for release of lariat-intron from the spliceosome. J Biol Chem. 2002;277:17743-50 pubmed
    ..We show that the lariat-intron is not accessible to debranching by purified Dbr1 when it is held in the T123A-arrested splicing complex. Our results define a new ATP-dependent step of splicing that is catalyzed by Prp43. ..
  81. Ryan K, Calvo O, Manley J. Evidence that polyadenylation factor CPSF-73 is the mRNA 3' processing endonuclease. RNA. 2004;10:565-73 pubmed
    ..Taken together, the available data provide strong evidence that CPSF-73 is the 3' processing endonuclease. ..
  82. Hon T, Lee H, Hu Z, Iyer V, Zhang L. The heme activator protein Hap1 represses transcription by a heme-independent mechanism in Saccharomyces cerevisiae. Genetics. 2005;169:1343-52 pubmed
    ..Further, we found that repression by Hap1 requires the function of Hsp70 (Ssa), but not Hsp90. These results show that Hap1 binds to its own promoter and represses transcription in a heme-independent but Hsp70-dependent manner. ..
  83. Ahn S, Diaz R, Grunstein M, Allis C. Histone H2B deacetylation at lysine 11 is required for yeast apoptosis induced by phosphorylation of H2B at serine 10. Mol Cell. 2006;24:211-20 pubmed
    ..Together, these studies underscore a concerted series of enzyme reactions governing histone modifications that promote a switch from cell proliferation to cell death. ..
  84. Rauceo J, Blankenship J, Fanning S, Hamaker J, Deneault J, Smith F, et al. Regulation of the Candida albicans cell wall damage response by transcription factor Sko1 and PAS kinase Psk1. Mol Biol Cell. 2008;19:2741-51 pubmed publisher
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