Genomes and Genes
Experts and Doctors on saccharomyces cerevisiae in New York, United States
Locale: New York, United States
Topic: saccharomyces cerevisiae
Publications360 found, 100 shown here
- Smith J, Rothstein R. An allele of RFA1 suppresses RAD52-dependent double-strand break repair in Saccharomyces cerevisiae. Genetics. 1999;151:447-58 pubmed..On the basis of these results, we propose that in the absence of wild-type Rad52, the interaction of RP-A with single-stranded DNA inhibits strand annealing, and that this inhibition is overcome by the rfa1-D228Y mutation. ..
- Liang X, Jackson S, Seaman M, Brown K, Kempkes B, Hibshoosh H, et al. Induction of autophagy and inhibition of tumorigenesis by beclin 1. Nature. 1999;402:672-6 pubmed..These findings suggest that decreased expression of autophagy proteins may contribute to the development or progression of breast and other human malignancies. ..
- Cross F, Levine K. Genetic analysis of the relationship between activation loop phosphorylation and cyclin binding in the activation of the Saccharomyces cerevisiae Cdc28p cyclin-dependent kinase. Genetics. 2000;154:1549-59 pubmed..This conclusion was supported by analysis of suppressors of a mutation in the Cdk phosphothreonine-binding pocket created by cyclin binding. ..
- Krogh B, Llorente B, Lam A, Symington L. Mutations in Mre11 phosphoesterase motif I that impair Saccharomyces cerevisiae Mre11-Rad50-Xrs2 complex stability in addition to nuclease activity. Genetics. 2005;171:1561-70 pubmed..Our results show that the structural integrity of the Mre11-Rad50-Xrs2 complex is more important than the catalytic activity of the Mre11 nuclease for the overall functions of the complex in vegetative cells. ..
- Pattingre S, Tassa A, Qu X, Garuti R, Liang X, Mizushima N, et al. Bcl-2 antiapoptotic proteins inhibit Beclin 1-dependent autophagy. Cell. 2005;122:927-39 pubmed..This antiautophagy function of Bcl-2 may help maintain autophagy at levels that are compatible with cell survival, rather than cell death. ..
- Wang L, Schwer B, Englert M, Beier H, Shuman S. Structure-function analysis of the kinase-CPD domain of yeast tRNA ligase (Trl1) and requirements for complementation of tRNA splicing by a plant Trl1 homolog. Nucleic Acids Res. 2006;34:517-27 pubmed..The plant ligase, like yeast Trl1 but unlike T4 RNA ligase 1, requires a 2'-PO4 end for tRNA splicing in vivo. ..
- Zeng X, Neupert W, Tzagoloff A. The metalloprotease encoded by ATP23 has a dual function in processing and assembly of subunit 6 of mitochondrial ATPase. Mol Biol Cell. 2007;18:617-26 pubmed..Atp23p may, therefore, also be a chaperone, which in conjunction with Atp10p mediates the association of subunit 6 with the subunit 9 ring. ..
- García Rodríguez L, Gay A, Pon L. Puf3p, a Pumilio family RNA binding protein, localizes to mitochondria and regulates mitochondrial biogenesis and motility in budding yeast. J Cell Biol. 2007;176:197-207 pubmed..Thus, Puf3p is a mitochondrial protein that contributes to the biogenesis and motility of the organelle. ..
- García Rodríguez L, Crider D, Gay A, Salanueva I, Boldogh I, Pon L. Mitochondrial inheritance is required for MEN-regulated cytokinesis in budding yeast. Curr Biol. 2009;19:1730-5 pubmed publisher..Our studies identify a novel MEN-linked regulatory system that inhibits cytokinesis in response to defects in mitochondrial inheritance in budding yeast. ..
- ..This review broadly surveys the chemistries and chemical mechanisms of these enzymes. ..
- Aguilera A, Klein H. HPR1, a novel yeast gene that prevents intrachromosomal excision recombination, shows carboxy-terminal homology to the Saccharomyces cerevisiae TOP1 gene. Mol Cell Biol. 1990;10:1439-51 pubmed..We conclude that Hpr1 is a novel eucaryotic protein, mutation of which causes an increase in mitotic intrachromosomal excision recombination, and that it may be functionally related to an activity of the topoisomerase I protein. ..
- Brill S, Stillman B. Yeast replication factor-A functions in the unwinding of the SV40 origin of DNA replication. Nature. 1989;342:92-5 pubmed..Substitution of yRF-A in the complete SV40 replication system, however, results in reduced DNA replication, presumably due to a requirement for species-specific interactions between yeast RF-A and the DNA polymerase complex. ..
- Søgaard M, Tani K, Ye R, Geromanos S, Tempst P, Kirchhausen T, et al. A rab protein is required for the assembly of SNARE complexes in the docking of transport vesicles. Cell. 1994;78:937-48 pubmed
- Sherwood P, Tsang S, Osley M. Characterization of HIR1 and HIR2, two genes required for regulation of histone gene transcription in Saccharomyces cerevisiae. Mol Cell Biol. 1993;13:28-38 pubmed..The sequence of the HIR2 gene predicts a protein of 98 kDa. Both gene products contain nuclear targeting signals, and the Hir2 protein is localized in the nucleus. ..
- Neiman A, Stevenson B, Xu H, Sprague G, Herskowitz I, Wigler M, et al. Functional homology of protein kinases required for sexual differentiation in Schizosaccharomyces pombe and Saccharomyces cerevisiae suggests a conserved signal transduction module in eukaryotic organisms. Mol Biol Cell. 1993;4:107-20 pubmed..Consistent with this hypothesis, we show that a mammalian mitogen-activated protein (MAP) kinase, ERK2, can partially replace spk1 function in S. pombe. ..
- Takagaki Y, Manley J. RNA recognition by the human polyadenylation factor CstF. Mol Cell Biol. 1997;17:3907-14 pubmed..We discuss these results in terms of the function and evolution of mRNA 3'-end signals. ..
- Zou H, Rothstein R. Holliday junctions accumulate in replication mutants via a RecA homolog-independent mechanism. Cell. 1997;90:87-96 pubmed..The level of Holliday junctions is greatly reduced in rad52 mutants, but surprisingly, not in mutants defective in the three known mitotically expressed yeast RecA homologs. ..
- Zhang L, Hach A, Wang C. Molecular mechanism governing heme signaling in yeast: a higher-order complex mediates heme regulation of the transcriptional activator HAP1. Mol Cell Biol. 1998;18:3819-28 pubmed..Our studies provide significant insights into the molecular events leading to heme activation of HAP1 and may shed light on molecular mechanisms of various heme-controlled biological processes in diverse organisms. ..
- Strambio de Castillia C, Blobel G, Rout M. Proteins connecting the nuclear pore complex with the nuclear interior. J Cell Biol. 1999;144:839-55 pubmed..In addition, we suggest that the Tpr filaments could provide chromatin-free conduits or tracks to guide the efficient translocation of macromolecules between the nucleoplasm and the NPC. ..
- Carlson M. Glucose repression in yeast. Curr Opin Microbiol. 1999;2:202-7 pubmed..Important advances include evidence that Snf1 regulates the localization of the Mig1 repressor and that Snf1 functions at multiple points to control Cat8 and Sip4, the activators of gluconeogenic genes. ..
- Wiatrowski H, Carlson M. Yap1 accumulates in the nucleus in response to carbon stress in Saccharomyces cerevisiae. Eukaryot Cell. 2003;2:19-26 pubmed..Other stresses such as increased temperature, acidic pH, and ionic stress did not cause nuclear enrichment of GFP-Yap1. These findings suggest a role for Yap1 in the response to carbon stress. ..
- Xu W, Smith F, Subaran R, Mitchell A. Multivesicular body-ESCRT components function in pH response regulation in Saccharomyces cerevisiae and Candida albicans. Mol Biol Cell. 2004;15:5528-37 pubmed..These results indicate that the ESCRT complexes govern a specific signal transduction pathway and suggest that the MVB pathway may provide a signal that regulates pH-responsive transcription. ..
- Antúnez de Mayolo A, Lisby M, Erdeniz N, Thybo T, Mortensen U, Rothstein R. Multiple start codons and phosphorylation result in discrete Rad52 protein species. Nucleic Acids Res. 2006;34:2587-97 pubmed..We also show that the Rad52 protein can be translated from the last three start sites and expression from any one of them is sufficient for spontaneous recombination and the repair of gamma-ray-induced double-strand breaks. ..
- Napetschnig J, Blobel G, Hoelz A. Crystal structure of the N-terminal domain of the human protooncogene Nup214/CAN. Proc Natl Acad Sci U S A. 2007;104:1783-8 pubmed..We propose a mechanism by which the C-terminal peptide extension is involved in NPC assembly. ..
- Dimitri A, Goodenough A, Guengerich F, Broyde S, Scicchitano D. Transcription processing at 1,N2-ethenoguanine by human RNA polymerase II and bacteriophage T7 RNA polymerase. J Mol Biol. 2008;375:353-66 pubmed..Thus, our data suggest that this repair pathway is likely engaged in the clearance of the 1,N(2)-epsilon G from actively transcribed DNA...
- Liang K, Wang X, Li T. Robust discovery of periodically expressed genes using the laplace periodogram. BMC Bioinformatics. 2009;10:15 pubmed publisher..The Laplace periodogram is shown to perform well for both data with and without the presence of outliers, and also for data that are non-uniformly sampled. ..
- Schwer B, Schneider S, Pei Y, Aronova A, Shuman S. Characterization of the Schizosaccharomyces pombe Spt5-Spt4 complex. RNA. 2009;15:1241-50 pubmed publisher..pombe growth, but only when Spt4 was present. Synthetic lethality of the spt5(1-835) spt4Delta double mutant at 34 degrees C suggests that interaction of Spt4 with the central domain of Spt5 overlaps functionally with the Spt5 CTD. ..
- Jain R, Vanamee E, Dzikovski B, Buku A, Johnson R, Prakash L, et al. An iron-sulfur cluster in the polymerase domain of yeast DNA polymerase ?. J Mol Biol. 2014;426:301-8 pubmed publisher..Collectively, our work suggests that Pol? is perhaps more sensitive than other DNA polymerases to changes in oxidative stress in eukaryotic cells...
- Tzagoloff A, Shtanko A. Mitochondrial and cytoplasmic isoleucyl-, glutamyl- and arginyl-tRNA synthetases of yeast are encoded by separate genes. Eur J Biochem. 1995;230:582-6 pubmed
- Mao X, Schwer B, Shuman S. Yeast mRNA cap methyltransferase is a 50-kilodalton protein encoded by an essential gene. Mol Cell Biol. 1995;15:4167-74 pubmed..Our finding that the ABD1 gene is required for yeast growth provides the first genetic evidence that a cap methyltransferase (and, by inference, the cap methyl group) plays an essential role in cellular function in vivo. ..
- Di Como C, Bose R, Arndt K. Overexpression of SIS2, which contains an extremely acidic region, increases the expression of SWI4, CLN1 and CLN2 in sit4 mutants. Genetics. 1995;139:95-107 pubmed
- Treitel M, Carlson M. Repression by SSN6-TUP1 is directed by MIG1, a repressor/activator protein. Proc Natl Acad Sci U S A. 1995;92:3132-6 pubmed..Finally, LexA-MIG1 does not repress transcription in glucose-deprived cells, and MIG1 is differentially phosphorylated in response to glucose availability. We suggest a role for phosphorylation in regulating repression. ..
- Vallier L, Coons D, Bisson L, Carlson M. Altered regulatory responses to glucose are associated with a glucose transport defect in grr1 mutants of Saccharomyces cerevisiae. Genetics. 1994;136:1279-85 pubmed..We suggest that GRR1 affects the glucose sensing process and that the association between transport and regulation may reflect the involvement of a transporter in glucose sensing. ..
- Benham C. Duplex destabilization in superhelical DNA is predicted to occur at specific transcriptional regulatory regions. J Mol Biol. 1996;255:425-34 pubmed..The occurrence of the observed SIDD patterns provides a new approach to searching uncharacterized genomic sequences for transcriptionally active regions. ..
- Oehlen L, McKinney J, Cross F. Ste12 and Mcm1 regulate cell cycle-dependent transcription of FAR1. Mol Cell Biol. 1996;16:2830-7 pubmed..This indicates that transcription of FAR1 in the G1 phase is essential for accumulation of the protein and for pheromone-induced cell cycle arrest. ..
- Erdmann R, Blobel G. Identification of Pex13p a peroxisomal membrane receptor for the PTS1 recognition factor. J Cell Biol. 1996;135:111-21 pubmed
- Pagano M. Cell cycle regulation by the ubiquitin pathway. FASEB J. 1997;11:1067-75 pubmed..This pathway targets substrates containing one or more destruction box motif. ..
- Neff N, Ellis N, Ye T, Noonan J, Huang K, Sanz M, et al. The DNA helicase activity of BLM is necessary for the correction of the genomic instability of bloom syndrome cells. Mol Biol Cell. 1999;10:665-76 pubmed..BLM complements a phenotype of a Saccharomyces cerevisiae sgs1 top3 strain, and the missense alleles do not. This work demonstrates the importance of the enzymatic activity of BLM for its function and nuclear localization pattern. ..
- Sherwood P, Carlson M. Efficient export of the glucose transporter Hxt1p from the endoplasmic reticulum requires Gsf2p. Proc Natl Acad Sci U S A. 1999;96:7415-20 pubmed..These findings suggest that Gsf2p functions in the ER to promote the secretion of certain hexose transporters. ..
- Kuchin S, Treich I, Carlson M. A regulatory shortcut between the Snf1 protein kinase and RNA polymerase II holoenzyme. Proc Natl Acad Sci U S A. 2000;97:7916-20 pubmed..These results suggest that direct regulatory interactions between signal transduction pathways and RNA polymerase II holoenzyme provide a mechanism for transcriptional control in response to important signals. ..
- Vannier D, Damay P, Shore D. A role for Sds3p, a component of the Rpd3p/Sin3p deacetylase complex, in maintaining cellular integrity in Saccharomyces cerevisiae. Mol Genet Genomics. 2001;265:560-8 pubmed..Taken together, these data indicate that the transcription factor Swi6p and the Rpd3p-based deacetylase complex act in parallel pathways to activate genes required for cell wall biosynthesis. ..
- Shi W, Tanaka K, Crother T, Taylor M, Almo S, Schramm V. Structural analysis of adenine phosphoribosyltransferase from Saccharomyces cerevisiae. Biochemistry. 2001;40:10800-9 pubmed..Mutation of one loop residue (Tyr103Phe) increases k(cat) by 4-fold, implicating altered dynamics for the catalytic site loop. ..
- James S, Turner W, Schwer B. How Slu7 and Prp18 cooperate in the second step of yeast pre-mRNA splicing. RNA. 2002;8:1068-77 pubmed..All three proteins are released from the spliceosome after step 2 concomitant with release of mature mRNA. ..
- Alexandrov A, Martzen M, Phizicky E. Two proteins that form a complex are required for 7-methylguanosine modification of yeast tRNA. RNA. 2002;8:1253-66 pubmed..This functional two-protein family appears to be retained in eukaryotes, as expression of both corresponding human proteins, METTL1 and WDR4, is required for m7G-methyltransferase activity. ..
- Silva A, Xu X, Kim H, Fillingham J, Kislinger T, Mennella T, et al. The replication-independent histone H3-H4 chaperones HIR, ASF1, and RTT106 co-operate to maintain promoter fidelity. J Biol Chem. 2012;287:1709-18 pubmed publisher..We propose that nucleosome shielding is the primary means to maintain promoter fidelity, and histone replacement is most efficiently mediated in yeast cells by a HIR/Asf1/H3-H4/Rtt106 pathway. ..
- Yu X, Breitman M, Goldberg J. A structure-based mechanism for Arf1-dependent recruitment of coatomer to membranes. Cell. 2012;148:530-42 pubmed publisher..A bivalent GTP-dependent binding mode has implications for the dynamics of coatomer interaction with the Golgi and for the selection of cargo molecules. ..
- Wasmuth E, Lima C. Exo- and endoribonucleolytic activities of yeast cytoplasmic and nuclear RNA exosomes are dependent on the noncatalytic core and central channel. Mol Cell. 2012;48:133-44 pubmed publisher..These results suggest the exosome core and central channel is essential because it modulates each of the known RNase activities of the yeast RNA exosome. ..
- Macro L, Jaiswal J, Simon S. Dynamics of clathrin-mediated endocytosis and its requirement for organelle biogenesis in Dictyostelium. J Cell Sci. 2012;125:5721-32 pubmed publisher..We hypothesize that dependence of endocytosis on specific components of the CME pathway evolved later, as demonstrated by internalization independent of AP2 function...
- Liu X, Mitchell J, Wozniak R, Blobel G, Fan J. Structural evolution of the membrane-coating module of the nuclear pore complex. Proc Natl Acad Sci U S A. 2012;109:16498-503 pubmed publisher..Another contact region is situated toward the end of the helical region of Sp-Nup120(1-950). Our findings provide information about the evolution and the assembly of the coatomer module of the nuclear pore complex. ..
- Kim J, Kim J, McGinty R, Nguyen U, Muir T, Allis C, et al. The n-SET domain of Set1 regulates H2B ubiquitylation-dependent H3K4 methylation. Mol Cell. 2013;49:1121-33 pubmed publisher..As not all members of the H3K4 methyltransferase family contain n-SET domains, our studies draw attention to the n-SET domain as a predictor of an H2B ubiquitylation-sensing mechanism that leads to downstream H3K4 methylation. ..
- Bernstein K, Mimitou E, Mihalevic M, Chen H, Sunjaveric I, Symington L, et al. Resection activity of the Sgs1 helicase alters the affinity of DNA ends for homologous recombination proteins in Saccharomyces cerevisiae. Genetics. 2013;195:1241-51 pubmed publisher..We suggest a model where the role of Sgs1 in end resection along with Sae2 is important for removing Mre11 from DNA ends during repair. ..
- Bemena L, Mukama O, Neiman A, Li Z, Gao X, Nakanishi H. In vitro reconstitution of the yeast spore wall dityrosine layer discloses the mechanism of its assembly. J Biol Chem. 2017;292:15880-15891 pubmed publisher..In conclusion, our results provide insight into the mechanism of dityrosine layer formation, and the in vitro assay described here may be used to investigate additional mechanisms in spore wall assembly. ..
- Hardy C, Sussel L, Shore D. A RAP1-interacting protein involved in transcriptional silencing and telomere length regulation. Genes Dev. 1992;6:801-14 pubmed..By use of the two-hybrid system, we have isolated a mutation in RIF1 that partially restores the interaction with rap1s mutant proteins. ..
- Field J, Vojtek A, Ballester R, Bolger G, Colicelli J, Ferguson K, et al. Cloning and characterization of CAP, the S. cerevisiae gene encoding the 70 kd adenylyl cyclase-associated protein. Cell. 1990;61:319-27 pubmed
- Kuchin S, Yeghiayan P, Carlson M. Cyclin-dependent protein kinase and cyclin homologs SSN3 and SSN8 contribute to transcriptional control in yeast. Proc Natl Acad Sci U S A. 1995;92:4006-10 pubmed..Genetic analysis indicates that the SSN3-SSN8 complex contributes to transcriptional repression of diversely regulated genes and also affects induction of the GAL1 promoter. ..
- Wu M, Repetto B, Glerum D, Tzagoloff A. Cloning and characterization of FAD1, the structural gene for flavin adenine dinucleotide synthetase of Saccharomyces cerevisiae. Mol Cell Biol. 1995;15:264-71 pubmed..This mechanism of suppression is supported by the presence of mitochondrial FAD synthetase activity in S. cerevisiae transformed with FAD1 on a high-copy-number plasmid but not in mitochondrial of a wild-type strain. ..
- Fan H, Klein H. Characterization of mutations that suppress the temperature-sensitive growth of the hpr1 delta mutant of Saccharomyces cerevisiae. Genetics. 1994;137:945-56 pubmed..The SOH1 gene has been cloned and sequenced. The null allele is 10-fold increased for recombination as measured by deletion of a leu2 direct repeat. ..
- Schuler E, Lin F, Worman H. Characterization of the human gene encoding LBR, an integral protein of the nuclear envelope inner membrane. J Biol Chem. 1994;269:11312-7 pubmed..These results are the first to demonstrate the structural organization of a vertebrate gene encoding an integral membrane protein of the nuclear envelope that may be a member of a family of polypeptides conserved in evolution. ..
- Pemberton L, Rosenblum J, Blobel G. A distinct and parallel pathway for the nuclear import of an mRNA-binding protein. J Cell Biol. 1997;139:1645-53 pubmed..Thus, at least two parallel pathways function in the import of mRNA-binding proteins, suggesting the need for the coordination of these pathways. ..
- Fang Y, Fliss A, Rao J, Caplan A. SBA1 encodes a yeast hsp90 cochaperone that is homologous to vertebrate p23 proteins. Mol Cell Biol. 1998;18:3727-34 pubmed..In vivo analysis of known Hsp90 substrate proteins revealed that Sba1 loss of function had only a mild effect on the activity of the tyrosine kinase v-Src and steroid hormone receptors. ..
- Rudge S, Cavenagh M, Kamath R, Sciorra V, Morris A, Kahn R, et al. ADP-Ribosylation factors do not activate yeast phospholipase Ds but are required for sporulation. Mol Biol Cell. 1998;9:2025-36 pubmed..The implications of these results are discussed with respect to current models of Arf signaling. ..
- Xiao Y, Mitchell A. Shared roles of yeast glycogen synthase kinase 3 family members in nitrogen-responsive phosphorylation of meiotic regulator Ume6p. Mol Cell Biol. 2000;20:5447-53 pubmed..These findings argue that nitrogen limitation governs Rim11p/Mck1p-dependent phosphorylation of Ume6p, which in turn is required for Ume6p-Ime1p interaction and meiotic gene activation. ..
- Hausmann S, Ho C, Schwer B, Shuman S. An essential function of Saccharomyces cerevisiae RNA triphosphatase Cet1 is to stabilize RNA guanylyltransferase Ceg1 against thermal inactivation. J Biol Chem. 2001;276:36116-24 pubmed..Genetic experiments implicate two peptide segments, (287)KPVSLYVW(295) and (337)WQNLKNLEQPLN(348), as likely constituents of the Cet1-binding site on Ceg1. ..
- Morgan E, Shah N, Symington L. The requirement for ATP hydrolysis by Saccharomyces cerevisiae Rad51 is bypassed by mating-type heterozygosity or RAD54 in high copy. Mol Cell Biol. 2002;22:6336-43 pubmed
- Merker R, Klein H. Role of transcription in plasmid maintenance in the hpr1Delta mutant of Saccharomyces cerevisiae. Mol Cell Biol. 2002;22:8763-73 pubmed..Models for the role of Hpr1p in mature mRNA formation and the cause of plasmid instability in the absence of the Hpr1 protein are discussed. ..
- Barrientos A, Zambrano A, Tzagoloff A. Mss51p and Cox14p jointly regulate mitochondrial Cox1p expression in Saccharomyces cerevisiae. EMBO J. 2004;23:3472-82 pubmed..The release of Mss51p from the complex occurs at a downstream step in the assembly pathway, probably catalyzed by Shy1p. ..
- Rauceo J, Blankenship J, Fanning S, Hamaker J, Deneault J, Smith F, et al. Regulation of the Candida albicans cell wall damage response by transcription factor Sko1 and PAS kinase Psk1. Mol Biol Cell. 2008;19:2741-51 pubmed publisher..Our results show that protein kinase Psk1 is required for expression of SKO1 and of Sko1-dependent genes in response to caspofungin. Thus Psk1 and Sko1 lie in a newly described signal transduction pathway. ..
- Dyavaiah M, Rooney J, Chittur S, Lin Q, Begley T. Autophagy-dependent regulation of the DNA damage response protein ribonucleotide reductase 1. Mol Cancer Res. 2011;9:462-75 pubmed publisher..Our results highlight roles for autophagy and TOR signaling in regulating a specific protein and demonstrate the importance of these pathways in optimizing the DNA damage response. ..
- Bernstein K, Juanchich A, Sunjevaric I, Rothstein R. The Shu complex regulates Rad52 localization during rDNA repair. DNA Repair (Amst). 2013;12:786-90 pubmed publisher..Our results suggest that in the absence of UAF30, the Shu complex plays a central role in Rad52 rDNA localization as long as Rad52 can be sumoylated. This discrimination is important for rDNA copy number homeostasis. ..
- Wang D, Mansisidor A, Prabhakar G, Hochwagen A. Condensin and Hmo1 Mediate a Starvation-Induced Transcriptional Position Effect within the Ribosomal DNA Array. Cell Rep. 2016;14:1010-1017 pubmed publisher..Starvation-induced nucleolar contraction and differential desilencing of the outer rDNA repeats may provide a mechanism to activate rDNA-encoded RNAPII transcription units without causing general rDNA instability. ..
- Tu J, Carlson M. REG1 binds to protein phosphatase type 1 and regulates glucose repression in Saccharomyces cerevisiae. EMBO J. 1995;14:5939-46 pubmed..These results suggest that REG1 is a regulatory subunit of PP1 that targets its activity to proteins in the glucose repression regulatory pathway. ..
- Yen W, Chico L, Lei M, Lue N. Telomerase regulatory subunit Est3 in two Candida species physically interacts with the TEN domain of TERT and telomeric DNA. Proc Natl Acad Sci U S A. 2011;108:20370-5 pubmed publisher..Our findings provide insights on the mechanisms and evolution of a widely conserved and functionally critical telomeric/telomerase component. ..
- Estruch F, Treitel M, Yang X, Carlson M. N-terminal mutations modulate yeast SNF1 protein kinase function. Genetics. 1992;132:639-50 pubmed..Finally, we present evidence that when the C terminus is deleted, overexpression of the SNF1 kinase domain is deleterious to the cell. ..
- Mitchell A, Bowdish K. Selection for early meiotic mutants in yeast. Genetics. 1992;131:65-72 pubmed..Suppressors of IME1 toxicity include recessive mutations in two genes, RIM11 and RIM16 (Regulator of Inducer of Meiosis), that are required for IME1 to activate IME2 expression. RIM11 maps near CIN4 on chromosome XIII. ..
- Russnak R, Nehrke K, Platt T. REF2 encodes an RNA-binding protein directly involved in yeast mRNA 3'-end formation. Mol Cell Biol. 1995;15:1689-97 pubmed..As the first gene genetically identified to participate in mRNA 3'-end maturation prior to the final polyadenylation step, REF2 provides an ideal starting point for identifying related genes in this event. ..
- Rout M, Blobel G. Isolation of the yeast nuclear pore complex. J Cell Biol. 1993;123:771-83 pubmed..It retains all but one of the eight known NPC proteins. In addition it contains as many as 80 uncharacterized proteins that are candidate NPC proteins. ..
- Huang K, Symington L. Suppressors of a Saccharomyces cerevisiae pkc1 mutation identify alleles of the phosphatase gene PTC1 and of a novel gene encoding a putative basic leucine zipper protein. Genetics. 1995;141:1275-85 pubmed..Because PTC1 was initially isolated as a component of the Hog1p MAPK pathway, it appears that these two MAPK cascades share a common regulatory feature. ..
- Chen J, Sadowski H, Kohanski R, Wang L. Stat5 is a physiological substrate of the insulin receptor. Proc Natl Acad Sci U S A. 1997;94:2295-300 pubmed..Taken together, our data strongly suggest that IR interacts with and phosphorylates Stat5 in vitro and in tissues physiologically sensitive to insulin. ..
- Johnson E, Blobel G. Ubc9p is the conjugating enzyme for the ubiquitin-like protein Smt3p. J Biol Chem. 1997;272:26799-802 pubmed..cerevisiae. These results suggest that, like ubiquitination, Smt3p conjugation may be a critical modification in cell cycle regulation. ..
- Vainberg I, Lewis S, Rommelaere H, Ampe C, Vandekerckhove J, Klein H, et al. Prefoldin, a chaperone that delivers unfolded proteins to cytosolic chaperonin. Cell. 1998;93:863-73 pubmed..We show that by directing target proteins to chaperonin, prefoldin promotes folding in an environment in which there are many competing pathways for nonnative proteins. ..
- Ho C, Schwer B, Shuman S. Genetic, physical, and functional interactions between the triphosphatase and guanylyltransferase components of the yeast mRNA capping apparatus. Mol Cell Biol. 1998;18:5189-98 pubmed..Hence, an RNA triphosphatase activity is essential for eukaryotic cell growth. This work highlights the potential for regulating mRNA cap formation through protein-protein interactions. ..
- Zhao X, Muller E, Rothstein R. A suppressor of two essential checkpoint genes identifies a novel protein that negatively affects dNTP pools. Mol Cell. 1998;2:329-40 pubmed..Finally, both in vivo and in vitro experiments show that Sml1 binds to Rnr1. We propose that Sml1 inhibits dNTP synthesis posttranslationally by binding directly to Rnr1 and that Mec1 and Rad53 are required to relieve this inhibition. ..
- Si K, Maitra U. The Saccharomyces cerevisiae homologue of mammalian translation initiation factor 6 does not function as a translation initiation factor. Mol Cell Biol. 1999;19:1416-26 pubmed..These results indicate that eIF6 does not act as a true translation initiation factor. Rather, the protein may be involved in the biogenesis and/or stability of 60S ribosomal subunits. ..
- Knoblauch R, Garabedian M. Role for Hsp90-associated cochaperone p23 in estrogen receptor signal transduction. Mol Cell Biol. 1999;19:3748-59 pubmed..Together, these results strongly suggest that p23 plays an important role in ER signal transduction. ..
- Smith J, Zou H, Rothstein R. Characterization of genetic interactions with RFA1: the role of RPA in DNA replication and telomere maintenance. Biochimie. 2000;82:71-8 pubmed..Furthermore, this result represents the first evidence of a role for RPA in telomere maintenance. ..
- Rozovsky S, Jogl G, Tong L, McDermott A. Solution-state NMR investigations of triosephosphate isomerase active site loop motion: ligand release in relation to active site loop dynamics. J Mol Biol. 2001;310:271-80 pubmed..The rate of ligand release is less than 1000 s(-1) at 0 degrees C and more than 1000 s(-1) at 30 degrees C. Therefore, loop motion and product release are probably concerted and likely to represent a rate limiting step for chemistry. ..
- Xu W, Mitchell A. Yeast PalA/AIP1/Alix homolog Rim20p associates with a PEST-like region and is required for its proteolytic cleavage. J Bacteriol. 2001;183:6917-23 pubmed..We suggest that Rim20p may serve as part of a scaffold that places Rim101p and Rim13p in close proximity. ..
- Barros M, Nobrega F, Tzagoloff A. Mitochondrial ferredoxin is required for heme A synthesis in Saccharomyces cerevisiae. J Biol Chem. 2002;277:9997-10002 pubmed..The increase in heme A is greater in mutants that overexpress Cox15p and ferredoxin. These results are consistent with a requirement of ferredoxin and indirectly of ferredoxin reductase in hydroxylation of heme O. ..