Genomes and Genes
Experts and Doctors on saccharomyces cerevisiae in Connecticut, United States
Locale: Connecticut, United States
Topic: saccharomyces cerevisiae
Publications159 found, 100 shown here
- Shi I, Hallwyl S, Seong C, Mortensen U, Rothstein R, Sung P. Role of the Rad52 amino-terminal DNA binding activity in DNA strand capture in homologous recombination. J Biol Chem. 2009;284:33275-84 pubmed publisher..These results provide evidence that DNA binding by the evolutionarily conserved amino terminus of Rad52 is needed for the capture of the second DNA end during homologous recombination. ..
- Liu Y, Gaines W, Callender T, Busygina V, Oke A, Sung P, et al. Down-regulation of Rad51 activity during meiosis in yeast prevents competition with Dmc1 for repair of double-strand breaks. PLoS Genet. 2014;10:e1004005 pubmed publisher..These results support the idea that down-regulation of Rad51 activity is important during meiosis to prevent Rad51 from competing with Dmc1 for repair of meiotic DSBs. ..
- Chua P, Roeder G. Tam1, a telomere-associated meiotic protein, functions in chromosome synapsis and crossover interference. Genes Dev. 1997;11:1786-800 pubmed..We discuss here possibilities for how a telomere-associated protein might function in chromosome synapsis and crossover interference. ..
- Van Horn D, Yoo C, Xue D, Shi H, Wolin S. The La protein in Schizosaccharomyces pombe: a conserved yet dispensable phosphoprotein that functions in tRNA maturation. RNA. 1997;3:1434-43 pubmed..Thus, although the La protein is dispensable for growth in these yeasts, both the structure of the protein and its function in pre-tRNA maturation have been highly conserved throughout evolution. ..
- Muth G, Chen L, Kosek A, Strobel S. pH-dependent conformational flexibility within the ribosomal peptidyl transferase center. RNA. 2001;7:1403-15 pubmed..Instead the data suggest that there is pH-dependent conformational flexibility within the peptidyl transferase center, the exact nature and physiological relevance of which is not known. ..
- Coelho P, Bryan A, Kumar A, Shadel G, Snyder M. A novel mitochondrial protein, Tar1p, is encoded on the antisense strand of the nuclear 25S rDNA. Genes Dev. 2002;16:2755-60 pubmed
- Borneman A, Leigh Bell J, Yu H, Bertone P, Gerstein M, Snyder M. Target hub proteins serve as master regulators of development in yeast. Genes Dev. 2006;20:435-48 pubmed..Our results indicate that target hubs can serve as master regulators whose activity is sufficient for the induction of complex developmental responses and therefore represent important regulatory nodes in biological networks. ..
- Li J, Hooker G, Roeder G. Saccharomyces cerevisiae Mer2, Mei4 and Rec114 form a complex required for meiotic double-strand break formation. Genetics. 2006;173:1969-81 pubmed..Mer2 does not show significant colocalization with Mre11 or Rec102 and Mer2 does not co-immunoprecipitate with Rec102. We propose that Mer2, Mei4, and Rec114 form a distinct complex required for DSB formation. ..
- Li J, Agarwal S, Roeder G. SSP2 and OSW1, two sporulation-specific genes involved in spore morphogenesis in Saccharomyces cerevisiae. Genetics. 2007;175:143-54 pubmed..We propose that Ssp2 plays a role in vesicle fusion during PSM formation. ..
- Kreft S, Hochstrasser M. An unusual transmembrane helix in the endoplasmic reticulum ubiquitin ligase Doa10 modulates degradation of its cognate E2 enzyme. J Biol Chem. 2011;286:20163-74 pubmed publisher..These results suggest that the TEB4-Doa10 domain regulates Doa10 association with the Ubc6 membrane anchor, thereby controlling the degradation rate of the E2. ..
- Long A, O Brien C, Malhotra K, Schwall C, Albert A, Watts A, et al. A detergent-free strategy for the reconstitution of active enzyme complexes from native biological membranes into nanoscale discs. BMC Biotechnol. 2013;13:41 pubmed publisher..This facile, single-step strategy obviates the requirement for detergents and yields membrane complexes suitable for structural and functional studies. ..
- West A, Clark D, Martin J, Neupert W, Hartl F, Horwich A. Two related genes encoding extremely hydrophobic proteins suppress a lethal mutation in the yeast mitochondrial processing enhancing protein. J Biol Chem. 1992;267:24625-33 pubmed..We suggest that SMF1 and SMF2 are mitochondrial membrane proteins that influence PEP-dependent protein import, possibly at the step of protein translocation. ..
- Burton J, Solomon M. D box and KEN box motifs in budding yeast Hsl1p are required for APC-mediated degradation and direct binding to Cdc20p and Cdh1p. Genes Dev. 2001;15:2381-95 pubmed..These results indicate that D box and KEN box motifs are important for direct binding to the APC machinery, leading to ubiquitination and subsequent protein degradation. ..
- Ling J, Peterson K, Simonovic I, Soll D, Simonovic M. The mechanism of pre-transfer editing in yeast mitochondrial threonyl-tRNA synthetase. J Biol Chem. 2012;287:28518-25 pubmed publisher..In conclusion, we propose that the plasticity of the aminoacylation site in MST1 allows binding of Ser-AMP and the appropriate positioning of the hydrolytic water molecule. ..
- Fröhlich F, Christiano R, Walther T. Native SILAC: metabolic labeling of proteins in prototroph microorganisms based on lysine synthesis regulation. Mol Cell Proteomics. 2013;12:1995-2005 pubmed publisher..As proof of principle, we have used nSILAC to globally analyze yeast proteome changes during salt stress. ..
- Datta B, Li B, Choubey D, Nallur G, Lengyel P. p202, an interferon-inducible modulator of transcription, inhibits transcriptional activation by the p53 tumor suppressor protein, and a segment from the p53-binding protein 1 that binds to p202 overcomes this inhibition. J Biol Chem. 1996;271:27544-55 pubmed..Expression of the 53BP1 segment binding to p202 overcame the inhibition by overexpressed p202 of the transcription of reporters mediated by the p53 or the AP-1 transcription factors and of the proliferation of yeast. ..
- Agarwal S, Roeder G. Zip3 provides a link between recombination enzymes and synaptonemal complex proteins. Cell. 2000;102:245-55 pubmed..We speculate that Zip3 is a component of recombination nodules and serves to link the initiation of synapsis to meiotic recombination...
- Du Y, Pypaert M, Novick P, Ferro Novick S. Aux1p/Swa2p is required for cortical endoplasmic reticulum inheritance in Saccharomyces cerevisiae. Mol Biol Cell. 2001;12:2614-28 pubmed..These findings suggest that Aux1p/Swa2p may be a bifunctional protein with roles in membrane traffic and cortical ER inheritance. In support of this hypothesis, we find that Aux1p/Swa2p localizes to ER membranes. ..
- Busygina V, Sehorn M, Shi I, Tsubouchi H, Roeder G, Sung P. Hed1 regulates Rad51-mediated recombination via a novel mechanism. Genes Dev. 2008;22:786-95 pubmed publisher..These findings shed light on the function of Hed1 and, importantly, unveil a novel mechanism for the regulation of homologous recombination. ..
- He C, Gong P, Hu B, Stewart D, Choi M, Choi A, et al. Identification of activating transcription factor 4 (ATF4) as an Nrf2-interacting protein. Implication for heme oxygenase-1 gene regulation. J Biol Chem. 2001;276:20858-65 pubmed..These results indicate that ATF4 regulates basal and CdCl(2)-induced expression of the ho-1 gene in a cell-specific manner and possibly in a complex with Nrf2. ..
- Ferreira T, Mason A, Pypaert M, Allen K, Slayman C. Quality control in the yeast secretory pathway: a misfolded PMA1 H+-ATPase reveals two checkpoints. J Biol Chem. 2002;277:21027-40 pubmed..Taken together, the results of this study establish Pma1-G381A as a useful new probe for the yeast secretory system. ..
- Sterling C, Sweasy J. DNA polymerase 4 of Saccharomyces cerevisiae is important for accurate repair of methyl-methanesulfonate-induced DNA damage. Genetics. 2006;172:89-98 pubmed..Our studies suggest that Pol4 is critical for accurate repair of DNA lesions induced by MMS. ..
- Barral Y, Mermall V, Mooseker M, Snyder M. Compartmentalization of the cell cortex by septins is required for maintenance of cell polarity in yeast. Mol Cell. 2000;5:841-51 pubmed..We propose that septins maintain cell polarity by specifying a boundary between cortical domains. ..
- Dohlman H, Song J, Ma D, Courchesne W, Thorner J. Sst2, a negative regulator of pheromone signaling in the yeast Saccharomyces cerevisiae: expression, localization, and genetic interaction and physical association with Gpa1 (the G-protein alpha subunit). Mol Cell Biol. 1996;16:5194-209 pubmed..These results demonstrate that Sst2 and Gpa1 interact physically and suggest that Sst2 is a direct negative regulator of Gpa1. ..
- Ferris H, Furukawa Y, Minamino T, Kroetz M, Kihara M, Namba K, et al. FlhB regulates ordered export of flagellar components via autocleavage mechanism. J Biol Chem. 2005;280:41236-42 pubmed..Finally, we provide evidence via peptide analysis and FlhB cleavage variants that the tertiary structure of FlhB plays a significant role in cleavage. Based on these results, we propose that FlhB cleavage is an autocatalytic process. ..
- Rojas M, Farr G, Fernandez C, Lauden L, McCormack J, Wolin S. Yeast Gis2 and its human ortholog CNBP are novel components of stress-induced RNP granules. PLoS ONE. 2012;7:e52824 pubmed publisher..These results implicate both Gis2 and CNBP in mRNA handling during stress. ..
- Sacher M, Stone S, Ferro Novick S. The synaptobrevin-related domains of Bos1p and Sec22p bind to the syntaxin-like region of Sed5p. J Biol Chem. 1997;272:17134-8 pubmed
- Beales P, Bergstrom C, Geerts N, Groves J, Vanderlick T. Single vesicle observations of the cardiolipin-cytochrome C interaction: induction of membrane morphology changes. Langmuir. 2011;27:6107-15 pubmed publisher..We discuss the possible biological implications of our observations in relation to the structure and function of mitochondria. ..
- Castrol C, Koretsky A, Domach M. NMR-Observed phosphate trafficking and polyphosphate dynamics in wild-type and vph1-1 mutant Saccharomyces cerevisae in response to stresses. Biotechnol Prog. 1999;15:65-73 pubmed..Overall, maintaining both polyphosphate and carbohydrate reserves may endow yeast with the ability to rapidly manage the extracellular environment. ..
- Diederich R, Matsuno K, Hing H, Artavanis Tsakonas S. Cytosolic interaction between deltex and Notch ankyrin repeats implicates deltex in the Notch signaling pathway. Development. 1994;120:473-81 pubmed..In addition to representing a new class of viable Notch allele, this mutation behaves similarly to mutations of deltex and further implicates the ankyrin repeats in Notch function. ..
- Horak C, Luscombe N, Qian J, Bertone P, Piccirrillo S, Gerstein M, et al. Complex transcriptional circuitry at the G1/S transition in Saccharomyces cerevisiae. Genes Dev. 2002;16:3017-33 pubmed
- Stone S, Sacher M, Mao Y, Carr C, Lyons P, Quinn A, et al. Bet1p activates the v-SNARE Bos1p. Mol Biol Cell. 1997;8:1175-81 pubmed..Genetic studies suggest that the interactions of Bet1p with Bos1p are regulated by the small GTP-binding protein Ypt1p. ..
- Stolc V, Altman S. Rpp1, an essential protein subunit of nuclear RNase P required for processing of precursor tRNA and 35S precursor rRNA in Saccharomyces cerevisiae. Genes Dev. 1997;11:2414-25 pubmed..Immunoprecipitated complexes cleave both yeast precursor tRNAs and precursor rRNAs. ..
- Dawson I, Roth S, Artavanis Tsakonas S. The Drosophila cell cycle gene fizzy is required for normal degradation of cyclins A and B during mitosis and has homology to the CDC20 gene of Saccharomyces cerevisiae. J Cell Biol. 1995;129:725-37 pubmed..Our data suggest that fzy function is required for normal cell cycle-regulated proteolysis that is necessary for successful progress through mitosis. ..
- Zheng P, Fay D, Burton J, Xiao H, Pinkham J, Stern D. SPK1 is an essential S-phase-specific gene of Saccharomyces cerevisiae that encodes a nuclear serine/threonine/tyrosine kinase. Mol Cell Biol. 1993;13:5829-42 pubmed
- Huber E, Heinemeyer W, Li X, Arendt C, Hochstrasser M, Groll M. A unified mechanism for proteolysis and autocatalytic activation in the 20S proteasome. Nat Commun. 2016;7:10900 pubmed publisher..This work provides insights into the basic mechanism of proteolysis and propeptide autolysis, as well as the evolutionary pressures that drove the proteasome to become a threonine protease. ..
- Nandabalan K, Roeder G. Binding of a cell-type-specific RNA splicing factor to its target regulatory sequence. Mol Cell Biol. 1995;15:1953-60 pubmed..RNase T1 footprinting indicates that the Mer1 protein contacts MER2 RNA at several points in the 5' exon and in the intron. Thus, Mer1 interacts directly with a regulatory element in MER2 RNA and promotes splicing. ..
- Kusmierczyk A, Kunjappu M, Funakoshi M, Hochstrasser M. A multimeric assembly factor controls the formation of alternative 20S proteasomes. Nat Struct Mol Biol. 2008;15:237-44 pubmed publisher..Our data demonstrate that Pba3-Pba4 orchestrates formation of a specific type of proteasome, the first example of a trans-acting factor that controls assembly of alternative proteasomal complexes. ..
- Nair J, Muller H, Peterson M, Novick P. Sec2 protein contains a coiled-coil domain essential for vesicular transport and a dispensable carboxy terminal domain. J Cell Biol. 1990;110:1897-909 pubmed..The Sec2 protein may function in conjunction with the Sec4 and Sec15 proteins to control vesicular traffic. ..
- Page B, Satterwhite L, Rose M, Snyder M. Localization of the Kar3 kinesin heavy chain-related protein requires the Cik1 interacting protein. J Cell Biol. 1994;124:507-19 pubmed..These data indicate that interaction between a putative kinesin heavy chain-related protein and another protein can determine the localization of motor activity and thereby affect the functional specificity of the motor complex. ..
- Govindan B, Bowser R, Novick P. The role of Myo2, a yeast class V myosin, in vesicular transport. J Cell Biol. 1995;128:1055-68 pubmed..Our observations are consistent with a role for Myo2 in transporting a class of secretory vesicles from the mother cell along actin cables into the bud. ..
- Rockmill B, Engebrecht J, Scherthan H, Loidl J, Roeder G. The yeast MER2 gene is required for chromosome synapsis and the initiation of meiotic recombination. Genetics. 1995;141:49-59 pubmed..We suggest that the primary defect in the mer2 mutant is in the initiation of meiotic genetic exchange. ..
- Dunbar D, Wormsley S, Agentis T, Baserga S. Mpp10p, a U3 small nucleolar ribonucleoprotein component required for pre-18S rRNA processing in yeast. Mol Cell Biol. 1997;17:5803-12 pubmed..These results reveal a novel protein essential for ribosome biogenesis and further elucidate the composition of the U3 snoRNP. ..
- Burchett S, Scott A, Errede B, Dohlman H. Identification of novel pheromone-response regulators through systematic overexpression of 120 protein kinases in yeast. J Biol Chem. 2001;276:26472-8 pubmed..These results reveal two new components of the pheromone-signaling cascade in yeast, each acting at a point downstream of the MAPK. ..
- Verkamp E, Jahn M, Jahn D, Kumar A, Soll D. Glutamyl-tRNA reductase from Escherichia coli and Synechocystis 6803. Gene structure and expression. J Biol Chem. 1992;267:8275-80 pubmed..and Beale, S. I. (1991) J. Biol. Chem. 266, 9740-9745). These experiments are the first direct demonstration of GluTR activity of the HemA protein and provide further evidence for two pathways of ALA formation in prokaryotes. ..
- Bacon R, Salminen A, Ruohola H, Novick P, Ferro Novick S. The GTP-binding protein Ypt1 is required for transport in vitro: the Golgi apparatus is defective in ypt1 mutants. J Cell Biol. 1989;109:1015-22 pubmed..We have also established genetic interactions between ypt1 and a subset of the other genes required for transport to and through the Golgi apparatus. ..
- Tsubouchi T, Zhao H, Roeder G. The meiosis-specific zip4 protein regulates crossover distribution by promoting synaptonemal complex formation together with zip2. Dev Cell. 2006;10:809-19 pubmed..This clustering depends on Zip1. Our results suggest an interaction between crossover pathways such that a protein (Zip1) acting in one pathway influences the distribution of crossovers promoted by a parallel (Mms4-dependent) pathway. ..
- Charette J, Baserga S. The DEAD-box RNA helicase-like Utp25 is an SSU processome component. RNA. 2010;16:2156-69 pubmed publisher..Thus, Utp25 is a novel SSU processome component that, along with Utp3, forms the first identified interactions among the different SSU processome subcomplexes. ..
- Prakash R, Krejci L, Van Komen S, Anke Schürer K, Kramer W, Sung P. Saccharomyces cerevisiae MPH1 gene, required for homologous recombination-mediated mutation avoidance, encodes a 3' to 5' DNA helicase. J Biol Chem. 2005;280:7854-60 pubmed..These results, thus, establish Mph1 as an ATP-dependent DNA helicase, and the availability of purified Mph1 should facilitate efforts at deciphering the role of this protein in homologous recombination and mutation avoidance. ..
- Rockmill B, Roeder G. A meiosis-specific protein kinase homolog required for chromosome synapsis and recombination. Genes Dev. 1991;5:2392-404 pubmed..The sequence of the MEK1 gene predicts a 56.8-kD protein with homology to serine-threonine protein kinases. The MEK1 gene maps to chromosome XV, 13 cM proximal to CDC64. Models for the function of the MEK1 gene product are proposed. ..
- Snyder M. The SPA2 protein of yeast localizes to sites of cell growth. J Cell Biol. 1989;108:1419-29 pubmed..Thus, SPA2 is a newly identified yeast gene that is involved in the direction and control of cell division, and whose gene product localizes to the site of cell growth. ..
- Yamamoto Y, Matsui M, Ang L, Deng X. Role of a COP1 interactive protein in mediating light-regulated gene expression in arabidopsis. Plant Cell. 1998;10:1083-94 pubmed..Taken together, our data indicate that CIP7 acts as a positive regulator of light-regulated genes and is a potential direct downstream target of COP1 for mediating light control of gene expression. ..
- Barrett J, Manning B, Snyder M. The Kar3p kinesin-related protein forms a novel heterodimeric structure with its associated protein Cik1p. Mol Biol Cell. 2000;11:2373-85 pubmed..These findings demonstrate that the Kar3-Cik1 complex has a novel heterodimeric structure not observed previously for kinesin complexes. ..
- Chen Q, Ma E, Behar K, Xu T, Haddad G. Role of trehalose phosphate synthase in anoxia tolerance and development in Drosophila melanogaster. J Biol Chem. 2002;277:3274-9 pubmed..We conclude that trehalose contributes to anoxia tolerance in flies; this protection is likely to be due to a reduction of protein aggregation. ..
- Burton J, Tsakraklides V, Solomon M. Assembly of an APC-Cdh1-substrate complex is stimulated by engagement of a destruction box. Mol Cell. 2005;18:533-42 pubmed..Moreover, an intact D box domain within a substrate was required to stimulate the association between the Cdh1p-substrate complex and the APC. ..
- Engebrecht J, Roeder G. Yeast mer1 mutants display reduced levels of meiotic recombination. Genetics. 1989;121:237-47 pubmed..Strains carrying disruptions of the MER1 gene are mitotically viable. The epistatic relationships between MER1 and previously characterized meiotic genes are described. ..
- Isaya G, Miklos D, Rollins R. MIP1, a new yeast gene homologous to the rat mitochondrial intermediate peptidase gene, is required for oxidative metabolism in Saccharomyces cerevisiae. Mol Cell Biol. 1994;14:5603-16 pubmed..A CEN plasmid-encoded YMIP protein restored normal MIP activity along with respiratory competence. Thus, YMIP is a functional homolog of RMIP and represents a new component of the yeast mitochondrial import machinery. ..
- Roemer T, Madden K, Chang J, Snyder M. Selection of axial growth sites in yeast requires Axl2p, a novel plasma membrane glycoprotein. Genes Dev. 1996;10:777-93 pubmed..We suggest that Axl2p acts as an anchor in the plasma membrane that helps direct new growth components and/or polarity establishment components to the cortical axial budding site. ..
- Mason A, Allen K, Slayman C. C-terminal truncations of the Saccharomyces cerevisiae PMA1 H+-ATPase have major impacts on protein conformation, trafficking, quality control, and function. Eukaryot Cell. 2014;13:43-52 pubmed publisher..Thus, the C terminus is much more than a simple appendage and profoundly influences the structure, biogenesis, and function of the yeast H(+)-ATPase. ..
- Menees T, Roeder G. MEI4, a yeast gene required for meiotic recombination. Genetics. 1989;123:675-82 pubmed..The mei4 mutation is able to rescue the spore-inviability phenotype of spo13 and 52 strains (i.e., mei4 spo13 rad52 mutants produce viable spores), indicating that MEI4 acts before RAD52 in the meiotic recombination pathway. ..
- Augagneur Y, Jaubert L, Schiavoni M, Pachikara N, Garg A, Usmani Brown S, et al. Identification and functional analysis of the primary pantothenate transporter, PfPAT, of the human malaria parasite Plasmodium falciparum. J Biol Chem. 2013;288:20558-67 pubmed publisher..The essential function of PfPAT and its ability to deliver both pantothenate and fenpropimorph makes it an attractive target for the development and delivery of new classes of antimalarial drugs...
- Yoo C, Wolin S. The yeast La protein is required for the 3' endonucleolytic cleavage that matures tRNA precursors. Cell. 1997;89:393-402 pubmed..We propose that binding by Lhp1p stabilizes pre-tRNAs in conformations that allow the 3' endonucleolytic cleavage to occur. ..
- Walch Solimena C, Collins R, Novick P. Sec2p mediates nucleotide exchange on Sec4p and is involved in polarized delivery of post-Golgi vesicles. J Cell Biol. 1997;137:1495-509 pubmed..We propose that Sec2p functions to couple the activation of Sec4p to the polarized delivery of vesicles to the site of exocytosis. ..
- Finger F, Novick P. Sec3p is involved in secretion and morphogenesis in Saccharomyces cerevisiae. Mol Biol Cell. 1997;8:647-62 pubmed..This suggests that SEC gene products are involved in determining the bud site and is consistent with a role for Sec3p in determining the correct site of exocytosis. ..
- San Segundo P, Roeder G. Pch2 links chromatin silencing to meiotic checkpoint control. Cell. 1999;97:313-24 pubmed..Under certain circumstances, Sir3-dependent localization of Pch2 to telomeres also provides checkpoint function. These unexpected findings link the nucleolus, chromatin silencing, and the pachytene checkpoint. ..
- Long K, Cedervall T, Walch Solimena C, Noe D, Huddleston M, Annan R, et al. Phosphorylation of the Saccharomyces cerevisiae La protein does not appear to be required for its functions in tRNA maturation and nascent RNA stabilization. RNA. 2001;7:1589-602 pubmed..Thus, although La proteins from yeast to humans are phosphoproteins, phosphorylation does not appear to be required for any of the identified functions of the S. cerevisiae protein. ..
- Serino G, Su H, Peng Z, Tsuge T, Wei N, Gu H, et al. Characterization of the last subunit of the Arabidopsis COP9 signalosome: implications for the overall structure and origin of the complex. Plant Cell. 2003;15:719-31 pubmed..Comparative analyses of the subunit interaction of CSN revealed a set of conserved subunit contacts and resulted in a model of CSN subunit topology, some aspects of which were substantiated by in vivo cross-link tests. ..
- Copela L, Fernandez C, Sherrer R, Wolin S. Competition between the Rex1 exonuclease and the La protein affects both Trf4p-mediated RNA quality control and pre-tRNA maturation. RNA. 2008;14:1214-27 pubmed publisher..Our experiments reveal that one consequence of Rex1p-dependent 3' trimming is the generation of aberrant RNAs that are targeted for decay by TRAMP. ..
- Paul A, Pollard T. Energetic requirements for processive elongation of actin filaments by FH1FH2-formins. J Biol Chem. 2009;284:12533-40 pubmed publisher..Because formins are most vulnerable to dissociation during translocation along the growing barbed end, we propose that the flexible linker influences the lifetime of this translocative state. ..
- Gerstein M. Patterns of protein-fold usage in eight microbial genomes: a comprehensive structural census. Proteins. 1998;33:518-34 pubmed..This implies there are no marked preferences for proteins with particular numbers of TM-helices (e.g. 7-TM) in microbial genomes. ..
- Cheng A, Ross K, Kaldis P, Solomon M. Dephosphorylation of cyclin-dependent kinases by type 2C protein phosphatases. Genes Dev. 1999;13:2946-57 pubmed..Furthermore, PP2C-like enzymes are the predominant phosphatases toward human Cdk2 in HeLa cell extracts, indicating that the substrate specificity of PP2Cs toward CDKs is evolutionarily conserved. ..
- Finger F, Novick P. Synthetic interactions of the post-Golgi sec mutations of Saccharomyces cerevisiae. Genetics. 2000;156:943-51 pubmed..The significance of these results is discussed in the context of both secretory pathway function and the utility of synthetic lethality studies and their interpretation. ..
- Guo W, Tamanoi F, Novick P. Spatial regulation of the exocyst complex by Rho1 GTPase. Nat Cell Biol. 2001;3:353-60 pubmed..These results reveal the action of parallel pathways for the polarized localization of the exocytic machinery, both of which are under the control of Rho1, a master regulator of cell polarity. ..
- Gogarten J, Hilario E. Inteins, introns, and homing endonucleases: recent revelations about the life cycle of parasitic genetic elements. BMC Evol Biol. 2006;6:94 pubmed..BMC Evol Biol 2006, 6:42) provide important stepping stones towards integrated studies on how these parasitic elements evolve through time together with, or despite, their hosts. ..
- Xue X, Raynard S, Busygina V, Singh A, Sung P. Role of replication protein A in double holliday junction dissolution mediated by the BLM-Topo III?-RMI1-RMI2 protein complex. J Biol Chem. 2013;288:14221-7 pubmed publisher..Examination of these mutants ascertains the significance of the RMI1-RPA interaction in dHJ dissolution. Our results thus implicate RPA as a cofactor of the BTR complex in dHJ dissolution. ..
- Turi T, Webster P, Rose J. Brefeldin A sensitivity and resistance in Schizosaccharomyces pombe. Isolation of multiple genes conferring resistance. J Biol Chem. 1994;269:24229-36 pubmed..The overexpression of pap1 probably confers BFA resistance indirectly by inducing expression of one or more other proteins. The isolation of several genes conferring BFA resistance suggests several mechanisms are involved. ..
- Burton J, Roberts D, Montaldi M, Novick P, De Camilli P. A mammalian guanine-nucleotide-releasing protein enhances function of yeast secretory protein Sec4. Nature. 1993;361:464-7 pubmed..The Mss4 protein also stimulates GDP release from Ypt1 and from the mammalian protein Rab3a, but not from Ras2. Mss4 shows sequence similarity to Dss4, a yeast protein with similar biochemical properties...
- Sheu Y, Santos B, Fortin N, Costigan C, Snyder M. Spa2p interacts with cell polarity proteins and signaling components involved in yeast cell morphogenesis. Mol Cell Biol. 1998;18:4053-69 pubmed..We thus propose that Spa2p, Pea2p, and Bud6p function together, perhaps as a complex, to promote polarized morphogenesis through regulation of the actin cytoskeleton and signaling pathways. ..
- Kabcenell A, Goud B, Northup J, Novick P. Binding and hydrolysis of guanine nucleotides by Sec4p, a yeast protein involved in the regulation of vesicular traffic. J Biol Chem. 1990;265:9366-72 pubmed..0012 min-1. By analogy with other classes of GTP binding proteins, both the nucleotide exchange and hydrolysis activities of Sec4p may be modulated in vivo to facilitate its role in the regulation of intercompartmental membrane traffic. ..
- Astatke M, Grindley N, Joyce C. Deoxynucleoside triphosphate and pyrophosphate binding sites in the catalytically competent ternary complex for the polymerase reaction catalyzed by DNA polymerase I (Klenow fragment). J Biol Chem. 1995;270:1945-54 pubmed..Mutations of Arg682, His734, and Tyr766 affect the binding of DNA, suggesting that these mutations, whose effect on dNTP binding is small, may influence dNTP binding indirectly via the positioning of the DNA template-primer. ..
- Du L, Novick P. Pag1p, a novel protein associated with protein kinase Cbk1p, is required for cell morphogenesis and proliferation in Saccharomyces cerevisiae. Mol Biol Cell. 2002;13:503-14 pubmed..Pag1p is a conserved protein. The homologs of Pag1p in other organisms are likely to form complexes with the Cbk1p-related kinases and function with those kinases in the same biological processes. ..
- Ahel I, Stathopoulos C, Ambrogelly A, Sauerwald A, Toogood H, Hartsch T, et al. Cysteine activation is an inherent in vitro property of prolyl-tRNA synthetases. J Biol Chem. 2002;277:34743-8 pubmed publisher..Therefore, ProRS exhibits a natural level of mischarging that is to date unequalled among the aminoacyl-tRNA synthetases...
- Hooker G, Roeder G. A Role for SUMO in meiotic chromosome synapsis. Curr Biol. 2006;16:1238-43 pubmed..The ubc9 mutant phenotype provides the first evidence for a cause-and-effect relationship between sumoylation and synapsis...
- Marotti L, Newitt R, Wang Y, Aebersold R, Dohlman H. Direct identification of a G protein ubiquitination site by mass spectrometry. Biochemistry. 2002;41:5067-74 pubmed..These findings indicate that the alpha-helical domain may serve to regulate the turnover of Gpa1. ..
- Chang W, Zaarour R, Reck Peterson S, Rinn J, Singer R, Snyder M, et al. Myo2p, a class V myosin in budding yeast, associates with a large ribonucleic acid-protein complex that contains mRNAs and subunits of the RNA-processing body. RNA. 2008;14:491-502 pubmed publisher..P-body disassembly is delayed in the motor mutant, myo2-66, indicating that Myo2p may facilitate the release of mRNAs from the P-body. ..
- Trent J, Nimmesgern E, Wall J, Hartl F, Horwich A. A molecular chaperone from a thermophilic archaebacterium is related to the eukaryotic protein t-complex polypeptide-1. Nature. 1991;354:490-3 pubmed publisher..In Saccharomyces cerevisiae, TCP1 is an essential protein that may play a part in mitotic spindle formation. We suggest that TF55 in archaebacteria and TCP1 in the eukaryotic cytosol are members of a new class of molecular chaperones...
- Horwich A, Willison K. Protein folding in the cell: functions of two families of molecular chaperone, hsp 60 and TF55-TCP1. Philos Trans R Soc Lond B Biol Sci. 1993;339:313-25; discussion 325-6 pubmed..We review here studies of both hsp 60 and other family members, discussing their essential physiological roles and mechanism of action. ..
- Abeliovich H, Grote E, Novick P, Ferro Novick S. Tlg2p, a yeast syntaxin homolog that resides on the Golgi and endocytic structures. J Biol Chem. 1998;273:11719-27 pubmed..Based on these findings we propose that Tlg2p is a t-SNARE that functions in transport from the endosome to the late Golgi and on the endocytic pathway. ..
- Leu J, Chua P, Roeder G. The meiosis-specific Hop2 protein of S. cerevisiae ensures synapsis between homologous chromosomes. Cell. 1998;94:375-86 pubmed..We propose that the Hop2 protein functions to prevent synapsis between nonhomologous chromosomes. ..