Experts and Doctors on recombinant proteins in California, United States

Summary

Locale: California, United States
Topic: recombinant proteins

Top Publications

  1. Luznik L, Martone M, Kraus G, Zhang Y, Xu Y, Ellisman M, et al. Localization of human immunodeficiency virus Rev in transfected and virus-infected cells. AIDS Res Hum Retroviruses. 1995;11:795-804 pubmed
    ..Our observations are consistent with the proposed dual role of Rev in mRNA transport and splicing. ..
  2. Williams M, Washburn M, Hans M, Urrutia A, Brust P, Prodanovich P, et al. Structure and functional characterization of a novel human low-voltage activated calcium channel. J Neurochem. 1999;72:791-9 pubmed
    ..Thus, alpha1H-containing channels exhibit biophysical and pharmacological properties characteristic of low voltage-activated, or T-type, Ca2+ channels. ..
  3. Ben Efraim I, Gerace L. Gradient of increasing affinity of importin beta for nucleoporins along the pathway of nuclear import. J Cell Biol. 2001;152:411-7 pubmed
    ..These results indicate that nucleoporins themselves, as well as the nucleocytoplasmic compartmentalization of the Ran system, are likely to play an important role in conferring directionality to nuclear protein import. ..
  4. Robinson D, Ocon S, Rock R, Spudich J. Dynacortin is a novel actin bundling protein that localizes to dynamic actin structures. J Biol Chem. 2002;277:9088-95 pubmed
    ..These results indicate that cells have evolved different actin-filament cross-linking proteins with complementary cellular distributions that collaborate to orchestrate complex cell shape changes. ..
  5. Gale A, Tsavaler A, Griffin J. Molecular characterization of an extended binding site for coagulation factor Va in the positive exosite of activated protein C. J Biol Chem. 2002;277:28836-40 pubmed
    ..In conjunction with previously published work, these results define an extensive FVa binding site in the positive exosite of APC that is primarily involved in binding and cleaving at Arg(506) on FVa. ..
  6. Quintanar L, Stoj C, Wang T, Kosman D, Solomon E. Role of aspartate 94 in the decay of the peroxide intermediate in the multicopper oxidase Fet3p. Biochemistry. 2005;44:6081-91 pubmed
    ..These studies provide new insight into the role of D94 and proton involvement in the reductive cleavage of the O-O bond. ..
  7. Xu B, Fu Y, Liu Y, Agvanian S, Wirka R, Baum R, et al. The ESCRT-III pathway facilitates cardiomyocyte release of cBIN1-containing microparticles. PLoS Biol. 2017;15:e2002354 pubmed publisher
    ..This study links the BAR protein superfamily to the ESCRT pathway for MP biogenesis in mammalian cardiac ventricular cells, identifying elements of a pathway by which cytoplasmic cBIN1 is released into blood. ..
  8. Dintzis S, Velculescu V, Pfeffer S. Receptor extracellular domains may contain trafficking information. Studies of the 300-kDa mannose 6-phosphate receptor. J Biol Chem. 1994;269:12159-66 pubmed
    ..These experiments suggest that the presence of the mannose 6-phosphate receptor extracellular domain may interfere with the rapid recycling of receptors from early endosomes to the cell surface and detain receptors within endosomes. ..
  9. McCutchen Maloney S, Giannecchini C, Hwang M, Thelen M. Domain mapping of the DNA binding, endonuclease, and ERCC1 binding properties of the human DNA repair protein XPF. Biochemistry. 1999;38:9417-25 pubmed
    ..A working model for the XPF protein is presented illustrating the function of XPF in the nucleotide excision pathway and depicting the two functional domains interacting with DNA and ERCC1. ..

More Information

Publications351 found, 100 shown here

  1. Yang B, Steegmaier M, Gonzalez L, Scheller R. nSec1 binds a closed conformation of syntaxin1A. J Cell Biol. 2000;148:247-52 pubmed
    ..These results support the hypothesis that Sec1 proteins function as syntaxin chaperons during vesicle docking, priming, and membrane fusion. ..
  2. Jez J, Noel J. Reaction mechanism of chalcone isomerase. pH dependence, diffusion control, and product binding differences. J Biol Chem. 2002;277:1361-9 pubmed
  3. Calderwood D, Yan B, de Pereda J, Alvarez B, Fujioka Y, Liddington R, et al. The phosphotyrosine binding-like domain of talin activates integrins. J Biol Chem. 2002;277:21749-58 pubmed
    ..These resemblances suggest that the activation of integrins requires the presence of a beta turn at NPX(Y/F) motifs conserved in integrin beta cytoplasmic domains. ..
  4. Bassler B, Wright M, Silverman M. Sequence and function of LuxO, a negative regulator of luminescence in Vibrio harveyi. Mol Microbiol. 1994;12:403-12 pubmed
  5. Kiefer P, Grimshaw C, Whiteley J. The comparative interaction of quinonoid (6R)-dihydrobiopterin and an alternative dihydropterin substrate with wild-type and mutant rat dihydropteridine reductases. Biochemistry. 1997;36:9438-45 pubmed
    ..The location of this loop within the three-dimensional structure is consistent with putative substrate binding loops for other members of the short chain dehydrogenase/reductase (SDR) family, which includes dihydropteridine reductase. ..
  6. Lim R, Halpain S. Regulated association of microtubule-associated protein 2 (MAP2) with Src and Grb2: evidence for MAP2 as a scaffolding protein. J Biol Chem. 2000;275:20578-87 pubmed
  7. Sasaki Y, Rothe T, Premkumar L, Das S, Cui J, Talantova M, et al. Characterization and comparison of the NR3A subunit of the NMDA receptor in recombinant systems and primary cortical neurons. J Neurophysiol. 2002;87:2052-63 pubmed
    ..Finally, a new longer splice variant of NR3A has been cloned and found to be expressed in rodent cortical neurons by single-cell RT-PCR and in situ hybridization. ..
  8. Kumagai J, Hsu S, Matsumi H, Roh J, Fu P, Wade J, et al. INSL3/Leydig insulin-like peptide activates the LGR8 receptor important in testis descent. J Biol Chem. 2002;277:31283-6 pubmed
    ..Demonstration of INSL3 as the ligand for LGR8 facilitates understanding of the mechanism of testis descent and allows studies on the role of INSL3 in gonadal and other physiological processes. ..
  9. Lee J, Mandell E, Tucey T, Morris D, Lundblad V. The Est3 protein associates with yeast telomerase through an OB-fold domain. Nat Struct Mol Biol. 2008;15:990-7 pubmed
    ..Our analysis of Est3 may be instructive in generating comparable missense mutations on the surface of the OB-fold domain of TPP1. ..
  10. Wong G, Muller O, Clark R, Conroy L, Moran M, Polakis P, et al. Molecular cloning and nucleic acid binding properties of the GAP-associated tyrosine phosphoprotein p62. Cell. 1992;69:551-8 pubmed
    ..These data suggest that p120-GAP and p62 play a role in some aspect of mRNA processing or utilization and that this role may be regulated by tyrosine phosphorylation, and indirectly, by p21ras. ..
  11. Clipstone N, Crabtree G. Identification of calcineurin as a key signalling enzyme in T-lymphocyte activation. Nature. 1992;357:695-7 pubmed
  12. McCarter L. MotX, the channel component of the sodium-type flagellar motor. J Bacteriol. 1994;176:5988-98 pubmed
  13. Flaherty K, Zozulya S, Stryer L, McKay D. Three-dimensional structure of recoverin, a calcium sensor in vision. Cell. 1993;75:709-16 pubmed
    ..The other two EF hands have novel structural features that prevent or impair calcium binding. A concave hydrophobic surface formed by EF hands 1 and 2 may participate in the read out of calcium signals by recoverin and its homologs. ..
  14. Du X, Fox J, Pei S. Identification of a binding sequence for the 14-3-3 protein within the cytoplasmic domain of the adhesion receptor, platelet glycoprotein Ib alpha. J Biol Chem. 1996;271:7362-7 pubmed
    ..Similarity between the GPIb-alpha C-terminal sequence and the serine-rich regions of Raf and Bcr kinases suggests a possible serine-rich recognition motif for the 14-3-3 protein. ..
  15. Wei P, Garber M, Fang S, Fischer W, Jones K. A novel CDK9-associated C-type cyclin interacts directly with HIV-1 Tat and mediates its high-affinity, loop-specific binding to TAR RNA. Cell. 1998;92:451-62 pubmed
    ..Moreover, overexpression of human cyclin T rescues Tat activity in nonpermissive rodent cells. We propose that Tat directs cyclin T-CDK9 to RNAPII through cooperative binding to TAR RNA. ..
  16. van Meyel D, O Keefe D, Jurata L, Thor S, Gill G, Thomas J. Chip and apterous physically interact to form a functional complex during Drosophila development. Mol Cell. 1999;4:259-65 pubmed
    ..This model is confirmed by the activity of a chimeric fusion between Chip and Ap that reconstitutes the complex and rescues the ap mutant phenotype. ..
  17. Movassat J, Beattie G, Lopez A, Portha B, Hayek A. Keratinocyte growth factor and beta-cell differentiation in human fetal pancreatic endocrine precursor cells. Diabetologia. 2003;46:822-9 pubmed
    ..The growth promoting effect of KGF on beta cells occurred mainly through the activation of ductal cell proliferation and their subsequent differentiation into beta cells. ..
  18. Cheeseman I, Niessen S, Anderson S, Hyndman F, Yates J, Oegema K, et al. A conserved protein network controls assembly of the outer kinetochore and its ability to sustain tension. Genes Dev. 2004;18:2255-68 pubmed
    ..Thus, this protein network is a conserved constituent of the outer kinetochore, and the functions defined by our analysis in C. elegans are likely to be widely relevant. ..
  19. Bieschke J, Zhang Q, Powers E, Lerner R, Kelly J. Oxidative metabolites accelerate Alzheimer's amyloidogenesis by a two-step mechanism, eliminating the requirement for nucleation. Biochemistry. 2005;44:4977-83 pubmed
    ..Metabolite-initiated amyloidogenesis offers an explanation for how Abeta aggregation could occur at physiological nanomolar concentrations. ..
  20. Ruegg M, Tsim K, Horton S, Kroger S, Escher G, Gensch E, et al. The agrin gene codes for a family of basal lamina proteins that differ in function and distribution. Neuron. 1992;8:691-9 pubmed
  21. Yamaguchi Y, Ruoslahti E. Expression of human proteoglycan in Chinese hamster ovary cells inhibits cell proliferation. Nature. 1988;336:244-6 pubmed
    ..These results reveal a novel growth inhibitory mechanism which may be related to contact inhibition of cell proliferation. ..
  22. Vaughan J, Donaldson C, Bittencourt J, Perrin M, Lewis K, Sutton S, et al. Urocortin, a mammalian neuropeptide related to fish urotensin I and to corticotropin-releasing factor. Nature. 1995;378:287-92 pubmed
  23. Foletti D, Lin R, Finley M, Scheller R. Phosphorylated syntaxin 1 is localized to discrete domains along a subset of axons. J Neurosci. 2000;20:4535-44 pubmed
    ..These phosphosyntaxin domains may define fusion sites for a novel class of vesicles outside classical active zones. ..
  24. Lee S, Hatakeyama S, Yu S, Bao X, Ohyama C, Khoo K, et al. Core3 O-glycan synthase suppresses tumor formation and metastasis of prostate carcinoma PC3 and LNCaP cells through down-regulation of alpha2beta1 integrin complex. J Biol Chem. 2009;284:17157-69 pubmed publisher
    ..These findings indicate that addition of core3 O-glycans to beta1 and alpha2 integrin subunits in prostate cancer cells suppresses tumor formation and tumor metastasis. ..
  25. Thielges M, Chung J, Axup J, Fayer M. Influence of histidine tag attachment on picosecond protein dynamics. Biochemistry. 2011;50:5799-805 pubmed publisher
  26. Sheridan P, Sheline C, Cannon K, Voz M, Pazin M, Kadonaga J, et al. Activation of the HIV-1 enhancer by the LEF-1 HMG protein on nucleosome-assembled DNA in vitro. Genes Dev. 1995;9:2090-104 pubmed
    ..We conclude that transcription activation by LEF-1 in vitro is a chromatin-dependent process that requires a functional trans-activation domain in addition to the HMG domain. ..
  27. Hammond M, Shyamala V, Siani M, Gallegos C, Feucht P, Abbott J, et al. Receptor recognition and specificity of interleukin-8 is determined by residues that cluster near a surface-accessible hydrophobic pocket. J Biol Chem. 1996;271:8228-35 pubmed
    ..Thus this region contains a second site for IL-8 receptor recognition that, in combination with the Glu4-Leu5-Arg6 region, can modulate receptor binding affinity and IL8R1 specificity. ..
  28. Armstrong J, Emerson B. NF-E2 disrupts chromatin structure at human beta-globin locus control region hypersensitive site 2 in vitro. Mol Cell Biol. 1996;16:5634-44 pubmed
    ..Lastly, nucleosome disruption by NF-E2 is an ATP-dependent process, suggesting the involvement of energy-dependent nucleosome remodeling factors. ..
  29. Stuart D, Wittenberg C. CLB5 and CLB6 are required for premeiotic DNA replication and activation of the meiotic S/M checkpoint. Genes Dev. 1998;12:2698-710 pubmed
    ..We conclude that CLB5 and CLB6 are essential for premeiotic DNA replication and, consequently, for activation of a meiotic DNA replication checkpoint. ..
  30. Chen H, Lin R, Xie W, Wilpitz D, Evans R. Regulation of hormone-induced histone hyperacetylation and gene activation via acetylation of an acetylase. Cell. 1999;98:675-86 pubmed
    ..These results provide strong in vivo evidence that histone acetylation plays a key role in hormone-induced gene activation and define cofactor acetylation as a novel regulatory mechanism in hormonal signaling. ..
  31. Du L, Nakamura T, Moser B, Russell P. Retention but not recruitment of Crb2 at double-strand breaks requires Rad1 and Rad3 complexes. Mol Cell Biol. 2003;23:6150-8 pubmed
  32. Comoletti D, Flynn R, Jennings L, Chubykin A, Matsumura T, Hasegawa H, et al. Characterization of the interaction of a recombinant soluble neuroligin-1 with neurexin-1beta. J Biol Chem. 2003;278:50497-505 pubmed
    ..We show here that glycosylation processing of neuroligin, in addition to mRNA splicing and gene selection, contributes to the specificity of the neurexin-beta/neuroligin-1 association. ..
  33. Steffek M, Newton G, Av Gay Y, Fahey R. Characterization of Mycobacterium tuberculosis mycothiol S-conjugate amidase. Biochemistry. 2003;42:12067-76 pubmed
    ..Significant activity was also seen with the mycothiol S-conjugate of the antibiotic cerulenin as a substrate for Mca. ..
  34. Verdecia M, Larkin R, Ferrer J, Riek R, Chory J, Noel J. Structure of the Mg-chelatase cofactor GUN4 reveals a novel hand-shaped fold for porphyrin binding. PLoS Biol. 2005;3:e151 pubmed
  35. Levi B, Hyun J, Nelson E, Li S, Montoro D, Wan D, et al. Nonintegrating knockdown and customized scaffold design enhances human adipose-derived stem cells in skeletal repair. Stem Cells. 2011;29:2018-29 pubmed publisher
    ..This study therefore suggests that genetic targeting of hASCs combined with custom scaffold design can optimize hASCs for skeletal regenerative medicine. ..
  36. Guttman M, Komives E. The structure, dynamics, and binding of the LA45 module pair of the low-density lipoprotein receptor suggest an important role for LA4 in ligand release. Biochemistry. 2011;50:11001-8 pubmed publisher
    ..The ligand affinity of both modules is enhanced when the two are linked. LA4 is more flexible than LA5 and remains so even in the module pair, which likely is related to its weaker calcium binding affinity. ..
  37. Basco R, Segal M, Reed S. Negative regulation of G1 and G2 by S-phase cyclins of Saccharomyces cerevisiae. Mol Cell Biol. 1995;15:5030-42 pubmed
    ..Thus, Clb5 and Clb6 may stabilize S phase by promoting DNA replication while inhibiting other cell cycle activities. ..
  38. de Vries T, Srnka C, Palcic M, Swiedler S, van den Eijnden D, Macher B. Acceptor specificity of different length constructs of human recombinant alpha 1,3/4-fucosyltransferases. Replacement of the stem region and the transmembrane domain of fucosyltransferase V by protein A results in an enzyme with GDP-fucose hydrolyzin. J Biol Chem. 1995;270:8712-22 pubmed
    ..All three Fuc-Ts had an absolute requirement for a hydroxyl at C-6 of galactose in addition to the accepting hydroxyl at C-3 or C-4 of GlcNAc. ..
  39. Broome M, Hunter T. The PDGF receptor phosphorylates Tyr 138 in the c-Src SH3 domain in vivo reducing peptide ligand binding. Oncogene. 1997;14:17-34 pubmed
    ..However, Tyr 138 phosphorylation was found to diminish SH3 domain peptide ligand binding ability in vitro. ..
  40. Han J, Liu S, Rose D, Schlaepfer D, McDonald H, Ginsberg M. Phosphorylation of the integrin alpha 4 cytoplasmic domain regulates paxillin binding. J Biol Chem. 2001;276:40903-9 pubmed
    ..Consequently, alpha4 phosphorylation is a biochemical mechanism to modulate paxillin binding to alpha4 integrins with consequent regulation of alpha4 integrin-dependent cellular functions. ..
  41. Zhao X, Zhuang S, Chen Y, Boss G, Pilz R. Cyclic GMP-dependent protein kinase regulates CCAAT enhancer-binding protein beta functions through inhibition of glycogen synthase kinase-3. J Biol Chem. 2005;280:32683-92 pubmed
    ..We conclude that cGMP increases the DNA binding potential of C/EBPbeta by preventing the negative effects of GSK-3 phosphorylation. ..
  42. Meier J, Barrows Yano T, Foley T, Wike C, Burkart M. The unusual macrocycle forming thioesterase of mycolactone. Mol Biosyst. 2008;4:663-71 pubmed publisher
    ..These findings suggest that MLSA2 TE utilizes a unique biochemical mechanism for macrocycle formation. ..
  43. Rennie E, Hansen S, Baidoo E, Hadi M, Keasling J, Scheller H. Three members of the Arabidopsis glycosyltransferase family 8 are xylan glucuronosyltransferases. Plant Physiol. 2012;159:1408-17 pubmed publisher
    ..We also show that several related proteins, GUX2 to GUX5 and Plant Glycogenin-like Starch Initiation Protein6, are Golgi localized and that only two of these proteins, GUX2 and GUX4, have activity as xylan ?-glucuronosyltransferases. ..
  44. Krensky A, Nelson P. Expression of chemokine RANTES and production of monoclonal antibodies. Methods Enzymol. 1997;287:162-74 pubmed
    ..This chapter describes the various techniques used to make recombinant RANTES protein, to test its activity, and to generate monoclonal antibodies to assess RANTES protein cell distribution. ..
  45. Matter M, Zhang Z, Nordstedt C, Ruoslahti E. The alpha5beta1 integrin mediates elimination of amyloid-beta peptide and protects against apoptosis. J Cell Biol. 1998;141:1019-30 pubmed
    ..Thus, the alpha5beta1 integrin may protect against Abeta deposition and toxicity, which is a course of Alzheimer's disease lesions. ..
  46. Tzeng Y, Zhou X, Hoch J. Phosphorylation of the Spo0B response regulator phosphotransferase of the phosphorelay initiating development in Bacillus subtilis. J Biol Chem. 1998;273:23849-55 pubmed
  47. Zhou Q, Zhao J, Al Zoghaibi F, Zhou A, Wiedmer T, Silverman R, et al. Transcriptional control of the human plasma membrane phospholipid scramblase 1 gene is mediated by interferon-alpha. Blood. 2000;95:2593-9 pubmed
    ..Blood. 2000;95:2593-2599) ..
  48. Liu C, Schultz P. Recombinant expression of selectively sulfated proteins in Escherichia coli. Nat Biotechnol. 2006;24:1436-40 pubmed
    ..This general approach to the biosynthesis of sulfated proteins should facilitate further study and application of tyrosine sulfation. ..
  49. O Reilly M, Paulson J. Multivalent ligands for siglecs. Methods Enzymol. 2010;478:343-63 pubmed publisher
    ..This chapter describes the use of multivalent ligands to probe cell surfaces, as well as to investigate ligand binding to recombinant siglecs. ..
  50. Andrade L, Chan E, Raska I, Peebles C, Roos G, Tan E. Human autoantibody to a novel protein of the nuclear coiled body: immunological characterization and cDNA cloning of p80-coilin. J Exp Med. 1991;173:1407-19 pubmed
  51. Simon C, Frances A, Piquette G, el Danasouri I, Zurawski G, Dang W, et al. Embryonic implantation in mice is blocked by interleukin-1 receptor antagonist. Endocrinology. 1994;134:521-8 pubmed
  52. Hay J, Hirling H, Scheller R. Mammalian vesicle trafficking proteins of the endoplasmic reticulum and Golgi apparatus. J Biol Chem. 1996;271:5671-9 pubmed
    ..The distinct localizations of rsec22 and rbet1 may reflect their participation in opposite directions of membrane flow between the endoplasmic reticulum and Golgi apparatus. ..
  53. Akama T, Nakayama J, Nishida K, Hiraoka N, Suzuki M, McAuliffe J, et al. Human corneal GlcNac 6-O-sulfotransferase and mouse intestinal GlcNac 6-O-sulfotransferase both produce keratan sulfate. J Biol Chem. 2001;276:16271-8 pubmed
    ..This observation suggests that mouse intestinal GlcNAc 6-O-sulfotransferase is the orthologue of hCGn6ST and functions as a sulfotransferase to produce keratan sulfate in the cornea. ..
  54. Lupardus P, Byun T, Yee M, Hekmat Nejad M, Cimprich K. A requirement for replication in activation of the ATR-dependent DNA damage checkpoint. Genes Dev. 2002;16:2327-32 pubmed
    ..These data indicate that replication is required for activation of the DNA damage checkpoint and suggest a unifying model for ATR activation by diverse lesions during S phase. ..
  55. Kinukawa M, Nomura M, Vacquier V. A sea urchin sperm flagellar adenylate kinase with triplicated catalytic domains. J Biol Chem. 2007;282:2947-55 pubmed publisher
    ..Thus, Sp-AK could contribute substantially to ATP synthesis utilized for motility. Alternatively, Sp-AK could function in the removal of ADP, which is a potent inhibitor of dynein ATPase...
  56. Rabuka D, Rush J, DeHart G, Wu P, Bertozzi C. Site-specific chemical protein conjugation using genetically encoded aldehyde tags. Nat Protoc. 2012;7:1052-67 pubmed publisher
    ..The process of generating aldehyde-tagged protein followed by chemical conjugation and purification takes 20 d. ..
  57. von Zastrow M, Kobilka B. Antagonist-dependent and -independent steps in the mechanism of adrenergic receptor internalization. J Biol Chem. 1994;269:18448-52 pubmed
  58. Almenoff J, Jurka J, Schoolnik G. Induction of heat-stable enterotoxin receptor activity by a human Alu repeat. J Biol Chem. 1994;269:16610-7 pubmed
    ..An unrelated Alu-rich genomic clone did not confer ST binding, suggesting that there may be structural and functional specificity within individual Alu sequences. ..
  59. Kiefer P, Varughese K, Su Y, Xuong N, Chang C, Gupta P, et al. Altered structural and mechanistic properties of mutant dihydropteridine reductases. J Biol Chem. 1996;271:3437-44 pubmed
    ..This is of little value to dihydropteridine reductase but may be significant in the sequentially analogous short chain dehydrogenases/reductases, where a serine is the amino acid of choice for this position. ..
  60. Chen S, Sawchuk R, Brundage R, Horvath C, Mendenhall H, Gunther R, et al. Plasma and lymph pharmacokinetics of recombinant human interleukin-2 and polyethylene glycol-modified interleukin-2 in pigs. J Pharmacol Exp Ther. 2000;293:248-59 pubmed
    ..v. dosing, and IL-2 would be favored over PEG-IL-2 to maximize lymph and minimize plasma exposure. Because IL-2 efficacy may be related to lymph concentrations, dosing regimens can now be designed to test this hypothesis. ..
  61. Fearns C, Kline L, Gram H, Di Padova F, Zurini M, Han J, et al. Coordinate activation of endogenous p38alpha, beta, gamma, and delta by inflammatory stimuli. J Leukoc Biol. 2000;67:705-11 pubmed
    ..The kinetics of activation of each of the p38s were similar for each stimulus used, suggesting a common upstream activation pathway. Simultaneous activation of the p38s suggests that all four may be important in inflammation. ..
  62. Tarui T, Mazar A, Cines D, Takada Y. Urokinase-type plasminogen activator receptor (CD87) is a ligand for integrins and mediates cell-cell interaction. J Biol Chem. 2001;276:3983-90 pubmed
  63. Brive L, Takayama S, Briknarová K, Homma S, Ishida S, Reed J, et al. The carboxyl-terminal lobe of Hsc70 ATPase domain is sufficient for binding to BAG1. Biochem Biophys Res Commun. 2001;289:1099-105 pubmed
    ..This result suggests that the stabilizing contacts for docking of BAG1 to Hsc70 are located in the C-terminal lobe of the ATPase domain. These findings provide new insights into the role of co-chaperones as nucleotide exchange factors. ..
  64. Demarest S, Martinez Yamout M, Chung J, Chen H, Xu W, Dyson H, et al. Mutual synergistic folding in recruitment of CBP/p300 by p160 nuclear receptor coactivators. Nature. 2002;415:549-53 pubmed
    ..Our study uncovers a unique mechanism, called 'synergistic folding', through which p160 coactivators recruit CBP/p300 to allow transmission of the hormonal signal to the transcriptional machinery. ..
  65. Kong M, Wang C, Donoghue D. Interaction of fibroblast growth factor receptor 3 and the adapter protein SH2-B. A role in STAT5 activation. J Biol Chem. 2002;277:15962-70 pubmed
    ..Taken together, our results identify SH2-B beta as a novel FGFR3 binding partner that mediates signal transduction. ..
  66. Vora H, McIntire J, Kumar P, Deshpande M, Khosla C. A scaleable manufacturing process for pro-EP-B2, a cysteine protease from barley indicated for celiac sprue. Biotechnol Bioeng. 2007;98:177-85 pubmed
    ..15 h(-1). A lyophilization procedure was developed that yielded protein with 85% recoverable activity after 7 weeks of storage at room temperature. The process was successfully scaled up to 100 L with comparable purity and recovery. ..
  67. Partridge J, Madsen M, Ardi V, Papagiannakopoulos T, Kupriyanova T, Quigley J, et al. Functional analysis of matrix metalloproteinases and tissue inhibitors of metalloproteinases differentially expressed by variants of human HT-1080 fibrosarcoma exhibiting high and low levels of intravasation and metastasis. J Biol Chem. 2007;282:35964-77 pubmed
    ..Collectively, these findings demonstrate that tumor-derived MMPs may have protective functions in cancer cell intravasation, i.e. not promoting but rather catalytically interfering with the early stages of cancer dissemination. ..
  68. Shrestha E, Hussein M, Savas J, Ouimet M, Barrett T, Leone S, et al. Poly(ADP-ribose) Polymerase 1 Represses Liver X Receptor-mediated ABCA1 Expression and Cholesterol Efflux in Macrophages. J Biol Chem. 2016;291:11172-84 pubmed publisher
    ..These findings shed light on the important role of PARP-1 on LXR-regulated lipid homeostasis. Understanding the interplay between PARP-1 and LXR may provide insights into developing novel therapeutics for treating atherosclerosis. ..
  69. Hsu S, Kim Y, Li S, Durrant E, Pace R, Woods V, et al. Structural insights into glucan phosphatase dynamics using amide hydrogen-deuterium exchange mass spectrometry. Biochemistry. 2009;48:9891-902 pubmed publisher
    ..Therefore, our results suggest that these regions of the DSP participate in the presentation of the phosphoglucan to the active site and provide the first structural analysis and mode of action of this unique class of phosphatases. ..
  70. Wickham T, Filardo E, Cheresh D, Nemerow G. Integrin alpha v beta 5 selectively promotes adenovirus mediated cell membrane permeabilization. J Cell Biol. 1994;127:257-64 pubmed
    ..These studies provide evidence for the involvement of a cellular receptor in virus-mediated membrane permeabilization and suggest a novel biological role for integrin alpha v beta 5 in the infectious pathway of a human adenovirus. ..
  71. Mol C, Arvai A, Slupphaug G, Kavli B, Alseth I, Krokan H, et al. Crystal structure and mutational analysis of human uracil-DNA glycosylase: structural basis for specificity and catalysis. Cell. 1995;80:869-78 pubmed
    ..Specific binding of uracil flipped out from a DNA duplex provides a structural mechanism for damaged base recognition. ..
  72. Cui M, Parry G, Oeth P, Larson H, Smith M, Huang R, et al. Transcriptional regulation of the tissue factor gene in human epithelial cells is mediated by Sp1 and EGR-1. J Biol Chem. 1996;271:2731-9 pubmed
    ..These data indicated that TF gene expression in HeLa cells was regulated by both Sp1 and EGR-1. ..
  73. Horan T, Wen J, Narhi L, Parker V, Garcia A, Arakawa T, et al. Dimerization of the extracellular domain of granuloycte-colony stimulating factor receptor by ligand binding: a monovalent ligand induces 2:2 complexes. Biochemistry. 1996;35:4886-96 pubmed
    ..This is consistent with the idea that G-CSF binding alters the conformation of the receptor, resulting in an increase in receptor-receptor interactions. ..
  74. Bushnell D, Bamdad C, Kornberg R. A minimal set of RNA polymerase II transcription protein interactions. J Biol Chem. 1996;271:20170-4 pubmed
    ..Protein blotting revealed an interaction between the largest subunit of TFIIE and third largest subunit of TFIIH, which may be responsible for TFIIE binding to TFIIH. ..
  75. Richieri G, Ogata R, Kleinfeld A. Thermodynamic and kinetic properties of fatty acid interactions with rat liver fatty acid-binding protein. J Biol Chem. 1996;271:31068-74 pubmed
  76. Saphire A, Guan T, Schirmer E, Nemerow G, Gerace L. Nuclear import of adenovirus DNA in vitro involves the nuclear protein import pathway and hsc70. J Biol Chem. 2000;275:4298-304 pubmed
    ..However, recombinant transport factors that are sufficient for hexon import in permeabilized cells do not support DNA import, indicating that there are other as yet unidentified factors required for this process. ..
  77. Yokoyama K, Erickson H, Ikeda Y, Takada Y. Identification of amino acid sequences in fibrinogen gamma -chain and tenascin C C-terminal domains critical for binding to integrin alpha vbeta 3. J Biol Chem. 2000;275:16891-8 pubmed
  78. McRee D, Williams P, Sridhar V, Pastuszyn A, Bren K, Patel K, et al. Recombinant cytochrome rC557 obtained from Escherichia coli cells expressing a truncated Thermus thermophilus cycA gene. Heme inversion in an improperly matured protein. J Biol Chem. 2001;276:6537-44 pubmed
    ..The unusual spectral properties of rC(557) are rationalized in terms of this structure. ..
  79. Mowla S, Farhadi H, Pareek S, Atwal J, Morris S, Seidah N, et al. Biosynthesis and post-translational processing of the precursor to brain-derived neurotrophic factor. J Biol Chem. 2001;276:12660-6 pubmed
    ..These data suggest that 28-kDa pro-BDNF is not an obligatory intermediate in the formation of the 14-kDa form in the constitutive secretory pathway. ..
  80. Kehlenbach R, Assheuer R, Kehlenbach A, Becker J, Gerace L. Stimulation of nuclear export and inhibition of nuclear import by a Ran mutant deficient in binding to Ran-binding protein 1. J Biol Chem. 2001;276:14524-31 pubmed
    ..These results provide physiological evidence that release of Ran-GTP from importin beta by RanBP1 and importin alpha is critical for the recycling of importin beta to a transport-competent state. ..
  81. Bonfoco E, Li E, Kolbinger F, Cooper N. Characterization of a novel proapoptotic caspase-2- and caspase-9-binding protein. J Biol Chem. 2001;276:29242-50 pubmed
    ..These findings identify a novel proapoptotic caspase adaptor protein. ..
  82. Osborne M, Schnell J, Benkovic S, Dyson H, Wright P. Backbone dynamics in dihydrofolate reductase complexes: role of loop flexibility in the catalytic mechanism. Biochemistry. 2001;40:9846-59 pubmed
    ..Finally, the relaxation data provide evidence for long-range motional coupling between the adenosine binding loop and distant regions of the protein. ..
  83. Belogrudov G, Hatefi Y. Factor B and the mitochondrial ATP synthase complex. J Biol Chem. 2002;277:6097-103 pubmed
    ..A likely factor B gene composed of 5 exons has been identified on chromosome 14q21.3, and the functional role of factor B in the mammalian ATP synthase complex has been discussed. ..
  84. Voloudakis A, Malpica C, Aleman Verdaguer M, Stark D, Fauquet C, Beachy R. Structural characterization of Tobacco etch virus coat protein mutants. Arch Virol. 2004;149:699-712 pubmed
    ..These results suggest that the Asp198Glu mutation has an altered secondary structure which affects the capacity of the protein to polymerize but did not affect in vitro protein-RNA interactions. ..
  85. Denault J, Bekes M, Scott F, Sexton K, Bogyo M, Salvesen G. Engineered hybrid dimers: tracking the activation pathway of caspase-7. Mol Cell. 2006;23:523-33 pubmed
    ..However, we see no evidence for an analogous intermediate of the related executioner caspase-3. Our study demonstrates the efficiency by which the executioner caspases are activated in vivo. ..
  86. Mulyasasmita W, Lee J, Heilshorn S. Molecular-level engineering of protein physical hydrogels for predictive sol-gel phase behavior. Biomacromolecules. 2011;12:3406-11 pubmed publisher
  87. Hatton O, Lambert S, Krams S, Martinez O. Src kinase and Syk activation initiate PI3K signaling by a chimeric latent membrane protein 1 in Epstein-Barr virus (EBV)+ B cell lymphomas. PLoS ONE. 2012;7:e42610 pubmed publisher
    ..Fyn, Syk, and PI3K/Akt antagonists thus may present potential new therapeutic strategies that target the oncogene LMP1 for treatment of EBV+ B cell lymphomas. ..
  88. Li L, Yin Q, Kuss P, Maliga Z, Millán J, Wu H, et al. Hydrolysis of 2'3'-cGAMP by ENPP1 and design of nonhydrolyzable analogs. Nat Chem Biol. 2014;10:1043-8 pubmed publisher
    ..2'3'-cG(s)A(s)MP is therefore improved over natural 2'3'-cGAMP as a model agonist and has potential as a vaccine adjuvant and cancer therapeutic. ..
  89. Rodionova I, Zhang Z, Mehla J, Goodacre N, Babu M, Emili A, et al. The phosphocarrier protein HPr of the bacterial phosphotransferase system globally regulates energy metabolism by directly interacting with multiple enzymes in Escherichia coli. J Biol Chem. 2017;292:14250-14257 pubmed publisher
    ..These observations suggest that HPr serves as a directly interacting global regulator of carbon and energy metabolism and probably of other physiological processes in enteric bacteria. ..
  90. Affholter J, Hsieh C, Francke U, Roth R. Insulin-degrading enzyme: stable expression of the human complementary DNA, characterization of its protein product, and chromosomal mapping of the human and mouse genes. Mol Endocrinol. 1990;4:1125-35 pubmed
    ..In addition, expression of various site-directed mutants of IDE will aid in identifying the residues of IDE and protease III that are essential to the activity of this unique family of proteinases. ..
  91. Tracy S, van der Geer P, Hunter T. The receptor-like protein-tyrosine phosphatase, RPTP alpha, is phosphorylated by protein kinase C on two serines close to the inner face of the plasma membrane. J Biol Chem. 1995;270:10587-94 pubmed
    ..Our results suggest that activation of PKC by cytokines modulates RPTP alpha function in several different ways. ..
  92. Paschal B, Gerace L. Identification of NTF2, a cytosolic factor for nuclear import that interacts with nuclear pore complex protein p62. J Cell Biol. 1995;129:925-37 pubmed
    ..NTF2 interacts with at least one additional cytosolic transport activity, indicating that it could be part of a multicomponent system of cytosolic factors that assemble at the pore complex during nuclear import. ..