Genomes and Genes
Experts and Doctors on proteins in California, United States
Locale: California, United States
Publications275 found, 100 shown here
- Kaganovich D, Kopito R, Frydman J. Misfolded proteins partition between two distinct quality control compartments. Nature. 2008;454:1088-95 pubmed publisher..Our findings provide a framework for understanding the preferential accumulation of amyloidogenic proteins in inclusions linked to human disease. ..
- Fu G, Vallee S, Rybakin V, McGuire M, Ampudia J, Brockmeyer C, et al. Themis controls thymocyte selection through regulation of T cell antigen receptor-mediated signaling. Nat Immunol. 2009;10:848-56 pubmed publisher..In support of the idea that Themis is involved in TCR signaling, this protein was phosphorylated quickly after TCR stimulation and was needed for optimal TCR-driven calcium mobilization and activation of the kinase Erk. ..
- Mohan R, Mach K, Bercovici M, Pan Y, Dhulipala L, Wong P, et al. Clinical validation of integrated nucleic acid and protein detection on an electrochemical biosensor array for urinary tract infection diagnosis. PLoS ONE. 2011;6:e26846 pubmed publisher..This platform can be used for multiplexed detection of nucleic acid and protein as the next generation of urinary tract infection diagnostics. ..
- Staeheli P, Grob R, Meier E, Sutcliffe J, Haller O. Influenza virus-susceptible mice carry Mx genes with a large deletion or a nonsense mutation. Mol Cell Biol. 1988;8:4518-23 pubmed..We further show that the Mx mRNA levels in interferon-treated BALB/c and CBA/J cells are about 15-fold lower than in similarly treated Mx+ cells. This is probably due to decreased metabolic stabilities of the mutant mRNAs. ..
- Lu Q, Gore M, Zhang Q, Camenisch T, Boast S, Casagranda F, et al. Tyro-3 family receptors are essential regulators of mammalian spermatogenesis. Nature. 1999;398:723-8 pubmed..Additional observations indicate that these receptors may also be essential for the tropic maintenance of diverse cell types in the mature nervous, immune and reproductive systems. ..
- Luciano F, Zhai D, Zhu X, Bailly Maitre B, Ricci J, Satterthwait A, et al. Cytoprotective peptide humanin binds and inhibits proapoptotic Bcl-2/Bax family protein BimEL. J Biol Chem. 2005;280:15825-35 pubmed..Taken together, our results indicate that the inhibition of BimEL may contribute to the antiapoptotic properties of the HN peptide. ..
- Ackerson C, Jadzinsky P, Sexton J, Bushnell D, Kornberg R. Synthesis and bioconjugation of 2 and 3 nm-diameter gold nanoparticles. Bioconjug Chem. 2010;21:214-8 pubmed publisher..Conjugates with antibody Fc fragment could be used to identify TAP-tagged proteins of interest in electron micrographs, through the binding of a pair of particles to the pair of protein A domains in the TAP tag. ..
- Simon M, Dodson G, Rubin G. An SH3-SH2-SH3 protein is required for p21Ras1 activation and binds to sevenless and Sos proteins in vitro. Cell. 1993;73:169-77 pubmed..We have renamed the E(sev)2B locus downstream of receptor kinases (drk). ..
- Hsu S. Cloning of two novel mammalian paralogs of relaxin/insulin family proteins and their expression in testis and kidney. Mol Endocrinol. 1999;13:2163-74 pubmed..Identification of RIF polypeptides expands the family of relaxin- and insulin-like hormones and allows future elucidation of the physiological role and hormonal mechanisms for these tissue-specific factors. ..
- Asahara H, Tartare Deckert S, Nakagawa T, Ikehara T, Hirose F, Hunter T, et al. Dual roles of p300 in chromatin assembly and transcriptional activation in cooperation with nucleosome assembly protein 1 in vitro. Mol Cell Biol. 2002;22:2974-83 pubmed..As p300 acted cooperatively with NAP-1 in stimulating transcription from a chromatin template in vitro, our results suggest a dual role of NAP-1-p300 complexes in promoting chromatin assembly and transcriptional activation. ..
- Go E, Wikoff W, Shen Z, O Maille G, Morita H, Conrads T, et al. Mass spectrometry reveals specific and global molecular transformations during viral infection. J Proteome Res. 2006;5:2405-16 pubmed..Overall, the cellular transformation that occurs upon viral infection is a process involving hundreds of proteins and metabolites, many of which are structurally and functionally uncharacterized. ..
- Goetinck P, Stirpe N, Tsonis P, Carlone D. The tandemly repeated sequences of cartilage link protein contain the sites for interaction with hyaluronic acid. J Cell Biol. 1987;105:2403-8 pubmed..Polylysine could not inhibit the interaction of LP with HA. The results indicate that the sites for interaction with HA are in the tandemly repeated sequences of LP and that there are four potential sites available for that interaction. ..
- Simske J, Kaech S, Harp S, Kim S. LET-23 receptor localization by the cell junction protein LIN-7 during C. elegans vulval induction. Cell. 1996;85:195-204 pubmed..These results suggest that proper localization of LET-23 receptor to the Pn.p cell junctions is required for signaling activity. ..
- Takahashi R, Deveraux Q, Tamm I, Welsh K, Assa Munt N, Salvesen G, et al. A single BIR domain of XIAP sufficient for inhibiting caspases. J Biol Chem. 1998;273:7787-90 pubmed..These findings identify BIR2 as the minimal caspase-inhibitory domain of XIAP and indicate that a single BIR domain can be sufficient for binding and inhibiting caspases. ..
- Mer G, Bochkarev A, Gupta R, Bochkareva E, Frappier L, Ingles C, et al. Structural basis for the recognition of DNA repair proteins UNG2, XPA, and RAD52 by replication factor RPA. Cell. 2000;103:449-56 pubmed..Our findings support a hand-off model for the assembly and coordination of different components of the DNA repair machinery. ..
- Zapata J, Pawlowski K, Haas E, Ware C, Godzik A, Reed J. A diverse family of proteins containing tumor necrosis factor receptor-associated factor domains. J Biol Chem. 2001;276:24242-52 pubmed..We propose the moniker TEFs (TD-encompassing factors) for this large family of proteins. ..
- Pascual G, Fong A, Ogawa S, Gamliel A, Li A, Perissi V, et al. A SUMOylation-dependent pathway mediates transrepression of inflammatory response genes by PPAR-gamma. Nature. 2005;437:759-63 pubmed..This mechanism provides an explanation for how an agonist-bound nuclear receptor can be converted from an activator of transcription to a promoter-specific repressor of NF-kappaB target genes that regulate immunity and homeostasis. ..
- Yoh S, Lucas J, Jones K. The Iws1:Spt6:CTD complex controls cotranscriptional mRNA biosynthesis and HYPB/Setd2-mediated histone H3K36 methylation. Genes Dev. 2008;22:3422-34 pubmed publisher..Thus Iws1 connects two distinct CTD-binding proteins, Spt6 and HYPB/Setd2, in a megacomplex that affects mRNA export as well as the histone modification state of active genes. ..
- Simon G, Dix M, Cravatt B. Comparative assessment of large-scale proteomic studies of apoptotic proteolysis. ACS Chem Biol. 2009;4:401-8 pubmed publisher..We discuss the respective strengths of each proteomic method and the potential for these technologies to expand the scope and sensitivity of large-scale studies of proteolysis in biological systems. ..
- Allard J, Herbst R, Carroll P, Simon M. Mutational analysis of the SRC homology 2 domain protein-tyrosine phosphatase Corkscrew. J Biol Chem. 1998;273:13129-35 pubmed..In addition, we found that deletion of either the CSW protein- tyrosine phosphatase insert or the entire CSW carboxyl terminus, which includes a conserved DRK/GRB2 SH2 domain binding sequence, does not abolish CSW function. ..
- Zisch A, Kalo M, Chong L, Pasquale E. Complex formation between EphB2 and Src requires phosphorylation of tyrosine 611 in the EphB2 juxtamembrane region. Oncogene. 1998;16:2657-70 pubmed..Our mutational analysis also indicated that tyrosines 605 and 611 are important for EphB2 kinase activity. We propose Src kinase as a downstream effector that mediates the neuron's response to Eph receptor activation. ..
- Tamm I, Wang Y, Sausville E, Scudiero D, Vigna N, Oltersdorf T, et al. IAP-family protein survivin inhibits caspase activity and apoptosis induced by Fas (CD95), Bax, caspases, and anticancer drugs. Cancer Res. 1998;58:5315-20 pubmed..Although quantitative differences may exist, these observations suggest commonality in the mechanisms used by IAP-family proteins to suppress apoptosis. ..
- Morris M, Heitz A, Mery J, Heitz F, Divita G. An essential phosphorylation-site domain of human cdc25C interacts with both 14-3-3 and cyclins. J Biol Chem. 2000;275:28849-57 pubmed..Combining our structural and biochemical data, we propose a detailed model of the molecular mechanism of cdc25C regulation by differential association with 14-3-3 and cdc2-cyclin B. ..
- Putnam N, Butts T, Ferrier D, Furlong R, Hellsten U, Kawashima T, et al. The amphioxus genome and the evolution of the chordate karyotype. Nature. 2008;453:1064-71 pubmed publisher..These genome-scale events shaped the vertebrate genome and provided additional genetic variation for exploitation during vertebrate evolution...
- Lopez M, Klein A, Dimbil U, Scott M. Anatomically defined neuron-based rescue of neurodegenerative Niemann-Pick type C disorder. J Neurosci. 2011;31:4367-78 pubmed publisher..This work may have broad implications for understanding the anatomical and cellular basis of neurological signs and symptoms of other neurodegenerative and lysosomal disorders. ..
- Nakamura T, Lipton S. Emerging role of protein-protein transnitrosylation in cell signaling pathways. Antioxid Redox Signal. 2013;18:239-49 pubmed publisher..Elucidation of additional transnitrosylation cascades will further our understanding of the enzymes that catalyze nitrosation, thereby contributing to NO-mediated signaling pathways. ..
- Hoppmann C, Wong A, Yang B, Li S, Hunter T, Shokat K, et al. Site-specific incorporation of phosphotyrosine using an expanded genetic code. Nat Chem Biol. 2017;13:842-844 pubmed publisher..We demonstrate its general compatibility with proteins of various sizes, phosphotyrosine sites and functions, and reveal a possible role of tyrosine phosphorylation in negative regulation of ubiquitination. ..
- Shatsky M, Saigo K, Burdach S, Leung L, Levitt L. Histidine-rich glycoprotein blocks T cell rosette formation and modulates both T cell activation and immunoregulation. J Biol Chem. 1989;264:8254-9 pubmed..HRGP is thus capable of modulating T cell activation and T cell immunoregulation; HRGP may function as a natural suppressive regulator of human T lymphocyte activation. ..
- Bowman E, Campbell J, Druey K, Scheschonka A, Kehrl J, Butcher E. Regulation of chemotactic and proadhesive responses to chemoattractant receptors by RGS (regulator of G-protein signaling) family members. J Biol Chem. 1998;273:28040-8 pubmed
- Sidow A, Bulotsky M, Kerrebrock A, Birren B, Altshuler D, Jaenisch R, et al. A novel member of the F-box/WD40 gene family, encoding dactylin, is disrupted in the mouse dactylaplasia mutant. Nat Genet. 1999;23:104-7 pubmed..In conjuction with recent biochemical studies, this report demonstrates the importance of this gene family in vertebrate embryonic development. ..
- Groppe J, Greenwald J, Wiater E, Rodriguez Leon J, Economides A, Kwiatkowski W, et al. Structural basis of BMP signalling inhibition by the cystine knot protein Noggin. Nature. 2002;420:636-42 pubmed..The scaffold of Noggin contains a cystine (the oxidized form of cysteine) knot topology similar to that of BMPs; thus, ligand and antagonist seem to have evolved from a common ancestral gene. ..
- Puto L, Pestonjamasp K, King C, Bokoch G. p21-activated kinase 1 (PAK1) interacts with the Grb2 adapter protein to couple to growth factor signaling. J Biol Chem. 2003;278:9388-93 pubmed..Blockade of this interaction decreased the epidermal growth factor-induced extension of membrane lamellae. Thus Grb2 may serve as an important mechanism for linking downstream PAK signaling to various upstream pathways. ..
- Chen H, Lilley C, Yu Q, Lee D, Chou J, Narvaiza I, et al. APOBEC3A is a potent inhibitor of adeno-associated virus and retrotransposons. Curr Biol. 2006;16:480-5 pubmed..They also highlight the functional differences between APOBEC3 proteins. The APOBEC3 family members have distinct functions and may have evolved to resist various classes of genetic elements. ..
- Nachury M. Tandem affinity purification of the BBSome, a critical regulator of Rab8 in ciliogenesis. Methods Enzymol. 2008;439:501-13 pubmed publisher..When combined with state-of-the art mass spectrometry, these methods can provide a nearly complete BBSome interactome containing factors such as Rabin8. ..
- Sengupta D, Heilshorn S. Protein-engineered biomaterials: highly tunable tissue engineering scaffolds. Tissue Eng Part B Rev. 2010;16:285-93 pubmed publisher..The modularity, tunability, and sequence specificity of protein-engineered biomaterials make them attractive candidates for use as substrates for a variety of tissue engineering applications. ..
- Ren G, Webster J, Liu Z, Zhang R, Miao Z, Liu H, et al. In vivo targeting of HER2-positive tumor using 2-helix affibody molecules. Amino Acids. 2012;43:405-13 pubmed publisher..Two-helix small protein scaffold holds great promise as a novel and robust platform for imaging and therapy applications. ..
- Christianson J, Olzmann J, Shaler T, Sowa M, Bennett E, Richter C, et al. Defining human ERAD networks through an integrative mapping strategy. Nat Cell Biol. 2011;14:93-105 pubmed publisher..This analysis supports an adaptive organization for the mammalian ERAD machinery and reveals a number of metazoan-specific genes not previously linked to ERAD. ..
- Toyama B, Hetzer M. Protein homeostasis: live long, won't prosper. Nat Rev Mol Cell Biol. 2013;14:55-61 pubmed publisher..This diverse group of long-lived proteins might be particularly prone to accumulation of damage and thus have a crucial role in the functional deterioration of key regulatory processes during ageing. ..
- Goodrich L, Johnson R, Milenkovic L, McMahon J, Scott M. Conservation of the hedgehog/patched signaling pathway from flies to mice: induction of a mouse patched gene by Hedgehog. Genes Dev. 1996;10:301-12 pubmed..As in flies, mouse ptc transcription appears to be indicative of hedgehog signal reception. The results support the existence of a conserved signaling pathway used for pattern formation in insects and mammals. ..
- Derry J, Wiedemann P, Blair P, Wang Y, Kerns J, Lemahieu V, et al. The mouse homolog of the Wiskott-Aldrich syndrome protein (WASP) gene is highly conserved and maps near the scurfy (sf) mutation on the X chromosome. Genomics. 1995;29:471-7 pubmed..Northern analysis of sf tissue samples indicated the presence of WASP mRNA in liver and skin, presumably as a consequence of lymphocytic infiltration, but non abnormalities in the amount or size of mRNA present. ..
- Shen M, Jones I, Mohrenweiser H. Nonconservative amino acid substitution variants exist at polymorphic frequency in DNA repair genes in healthy humans. Cancer Res. 1998;58:604-8 pubmed
- Zhao L, Donaldson C, Smith G, Vale W. The structures of the mouse and human urocortin genes (Ucn and UCN). Genomics. 1998;50:23-33 pubmed
- Goller M, Iacovoni J, Vogt P, Kruse U. Glutaredoxin is a direct target of oncogenic jun. Oncogene. 1998;16:2945-8 pubmed..Expression of the glutaredoxin mRNA could be induced by a Jun-estrogen receptor chimaera in the absence of de novo protein biosynthesis. Based on this observation we conclude that glutaredoxin is a direct target of v-Jun. ..
- Ulaner G, Hu J, Vu T, Giudice L, Hoffman A. Telomerase activity in human development is regulated by human telomerase reverse transcriptase (hTERT) transcription and by alternate splicing of hTERT transcripts. Cancer Res. 1998;58:4168-72 pubmed
- Guo B, Zhai D, Cabezas E, Welsh K, Nouraini S, Satterthwait A, et al. Humanin peptide suppresses apoptosis by interfering with Bax activation. Nature. 2003;423:456-61 pubmed..We speculate therefore that HN arose from mitochondria and transferred to the nuclear genome, providing a mechanism for protecting these organelles from Bax. ..
- Copp J, Manning G, Hunter T. TORC-specific phosphorylation of mammalian target of rapamycin (mTOR): phospho-Ser2481 is a marker for intact mTOR signaling complex 2. Cancer Res. 2009;69:1821-7 pubmed publisher..Thus, phospho-S2481 on mTOR serves as a biomarker for intact mTORC2 and its sensitivity to rapamycin. ..
- Morris G, Huey R, Lindstrom W, Sanner M, Belew R, Goodsell D, et al. AutoDock4 and AutoDockTools4: Automated docking with selective receptor flexibility. J Comput Chem. 2009;30:2785-91 pubmed publisher..We also report its utility in analysis of covalently bound ligands, using both a grid-based docking method and a modification of the flexible sidechain technique. ..
- Durrant J, Amaro R, Xie L, Urbaniak M, Ferguson M, Haapalainen A, et al. A multidimensional strategy to detect polypharmacological targets in the absence of structural and sequence homology. PLoS Comput Biol. 2010;6:e1000648 pubmed publisher..As it is capable of identifying potential secondary targets, the strategy described here may play a useful role in future efforts to reduce drug side effects and/or to increase polypharmacology. ..
- Ferreira J, Bôer B, Grinberg M, Brum P, Mochly Rosen D. Protein quality control disruption by PKCβII in heart failure; rescue by the selective PKCβII inhibitor, βIIV5-3. PLoS ONE. 2012;7:e33175 pubmed publisher..PQC disruption by increased PKCβII activity in vivo appears to contribute to the pathophysiology of heart failure, suggesting that PKCβII inhibition may benefit patients with heart failure. (218 words). ..
- Rabuka D, Rush J, DeHart G, Wu P, Bertozzi C. Site-specific chemical protein conjugation using genetically encoded aldehyde tags. Nat Protoc. 2012;7:1052-67 pubmed publisher..The process of generating aldehyde-tagged protein followed by chemical conjugation and purification takes 20 d. ..
- Wang Y, Fonslow B, Wong C, Nakorchevsky A, Yates J. Improving the comprehensiveness and sensitivity of sheathless capillary electrophoresis-tandem mass spectrometry for proteomic analysis. Anal Chem. 2012;84:8505-13 pubmed publisher..From comparative analysis of SPME-tITP-CE with direct injection CE, the SPME-tITP process improved comprehensiveness and sensitivity. ..
- Manley N, Caso J, Works M, Cutler A, Zemlyak I, Sun G, et al. Derivation of injury-responsive dendritic cells for acute brain targeting and therapeutic protein delivery in the stroke-injured rat. PLoS ONE. 2013;8:e61789 pubmed publisher..These results demonstrate a non-invasive method to target ischemic brain injury and may ultimately provide a way to selectively deliver therapeutic compounds to the injured brain. ..
- Gavis E, Hogness D. Phosphorylation, expression and function of the Ultrabithorax protein family in Drosophila melanogaster. Development. 1991;112:1077-93 pubmed
- Fritzler M, Hamel J, Ochs R, Chan E. Molecular characterization of two human autoantigens: unique cDNAs encoding 95- and 160-kD proteins of a putative family in the Golgi complex. J Exp Med. 1993;178:49-62 pubmed..Sequence analysis demonstrated that golgin-95 and golgin-160 share 43% sequence similarity and, therefore, may be functionally related proteins. ..
- Yeh T, Ogawa W, Danielsen A, Roth R. Characterization and cloning of a 58/53-kDa substrate of the insulin receptor tyrosine kinase. J Biol Chem. 1996;271:2921-8 pubmed..This sequence was utilized to isolate a cDNA clone that encodes a previously uncharacterized 53-kDa protein which, when expressed in mammalian cells, is tyrosine phosphorylated by the insulin receptor. ..
- Liu Y, Elly C, Yoshida H, Bonnefoy Berard N, Altman A. Activation-modulated association of 14-3-3 proteins with Cbl in T cells. J Biol Chem. 1996;271:14591-5 pubmed
- Schlaepfer D, Broome M, Hunter T. Fibronectin-stimulated signaling from a focal adhesion kinase-c-Src complex: involvement of the Grb2, p130cas, and Nck adaptor proteins. Mol Cell Biol. 1997;17:1702-13 pubmed..These results show that there are additional FN-stimulated pathways to ERK2 that do not involve Grb2 binding to FAK. ..
- Lopez Girona A, Furnari B, Mondesert O, Russell P. Nuclear localization of Cdc25 is regulated by DNA damage and a 14-3-3 protein. Nature. 1999;397:172-5 pubmed..Thus, Rad24 appears to function as an attachable nuclear-export signal that enhances the nuclear export of Cdc25 in response to DNA damage. ..
- Milenkovic L, Goodrich L, Higgins K, Scott M. Mouse patched1 controls body size determination and limb patterning. Development. 1999;126:4431-40 pubmed..The polydactyly was preceded by unexpected anterior limb bud transcription of Shh, so one function of ptc1 is to repress Shh expression in the anterior limb bud. ..
- Eystathioy T, Jakymiw A, Fujita D, Fritzler M, Chan E. Human autoantibodies to a novel Golgi protein golgin-67: high similarity with golgin-95/gm 130 autoantigen. J Autoimmun. 2000;14:179-87 pubmed..Golgin-67 is thus the smallest member of a growing family of Golgi autoantigens rich in alpha-helical coiled-coil domain. The current hypothesis for the generation of autoimmune antibody to the Golgi complex is discussed. ..
- Kain K, Klemke R. Inhibition of cell migration by Abl family tyrosine kinases through uncoupling of Crk-CAS complexes. J Biol Chem. 2001;276:16185-92 pubmed..Thus, Abl and Arg kinases play a critical role in preventing cell migration through regulation of Crk and CAS adaptor protein complexes, which are necessary for cell movement. ..
- Lu Z, Jiang G, Blume Jensen P, Hunter T. Epidermal growth factor-induced tumor cell invasion and metastasis initiated by dephosphorylation and downregulation of focal adhesion kinase. Mol Cell Biol. 2001;21:4016-31 pubmed
- Erickson G, Fuqua L, Shimasaki S. Analysis of spatial and temporal expression patterns of bone morphogenetic protein family members in the rat uterus over the estrous cycle. J Endocrinol. 2004;182:203-17 pubmed
- Nomura M, Vacquier V. Proteins associated with soluble adenylyl cyclase in sea urchin sperm flagella. Cell Motil Cytoskeleton. 2006;63:582-90 pubmed..The sAC-associated proteins could be important in linking membrane signal transduction to energy utilisation in the regulation of flagellar motility. ..
- Li W, Wooley J, Godzik A. Probing metagenomics by rapid cluster analysis of very large datasets. PLoS ONE. 2008;3:e3375 pubmed publisher..A Web server with our clustering results and annotations of predicted protein clusters is available online at http://tools.camera.calit2.net/gos under the CAMERA project. ..
- Kim M, Soontornniyomkij V, Ji B, ZHOU X. System-wide immunohistochemical analysis of protein co-localization. PLoS ONE. 2012;7:e32043 pubmed publisher..Repeated use of the same section could combine assessments of co-localization and differential expression of multiple proteins. ..
- Miller M, Duhl D, Vrieling H, Cordes S, Ollmann M, Winkes B, et al. Cloning of the mouse agouti gene predicts a secreted protein ubiquitously expressed in mice carrying the lethal yellow mutation. Genes Dev. 1993;7:454-67 pubmed..We speculate that dominant pleiotropic effects of Ay are caused by ectopic activation of a signaling pathway similar to that used during normal hair growth. ..
- Lim R, Halpain S. Regulated association of microtubule-associated protein 2 (MAP2) with Src and Grb2: evidence for MAP2 as a scaffolding protein. J Biol Chem. 2000;275:20578-87 pubmed
- Reimann J, Gardner B, Margottin Goguet F, Jackson P. Emi1 regulates the anaphase-promoting complex by a different mechanism than Mad2 proteins. Genes Dev. 2001;15:3278-85 pubmed..We find that Emi1 binds the substrate-binding region of Cdc20 and prevents substrate binding to the APC, illustrating a novel mechanism of APC inhibition. ..
- Salvesen G, Duckett C. IAP proteins: blocking the road to death's door. Nat Rev Mol Cell Biol. 2002;3:401-10 pubmed..Here, we review the functional and structural properties of this fascinating group of proteins, and of several recently identified IAP-binding factors that regulate IAP function. ..
- Paulmurugan R, Gambhir S. Novel fusion protein approach for efficient high-throughput screening of small molecule-mediating protein-protein interactions in cells and living animals. Cancer Res. 2005;65:7413-20 pubmed..00001 nmol/L) of rapamycin. For a similar fusion system employing split-EGFP, flow cytometry analysis showed significant level of rapamycin-induced complementation. ..
- Chamero P, Marton T, Logan D, Flanagan K, Cruz J, Saghatelian A, et al. Identification of protein pheromones that promote aggressive behaviour. Nature. 2007;450:899-902 pubmed..Our results substantiate the idea of MUP proteins as pheromone ligands that mediate male-male aggression through the accessory olfactory neural pathway...
- Zhang X, Facette M, Humphries J, Shen Z, Park Y, Sutimantanapi D, et al. Identification of PAN2 by quantitative proteomics as a leucine-rich repeat-receptor-like kinase acting upstream of PAN1 to polarize cell division in maize. Plant Cell. 2012;24:4577-89 pubmed publisher..Together, these results place PAN2 at the top of a cascade of events promoting the polarization of SMC divisions, potentially functioning to perceive or amplify GMC-derived polarizing cues. ..
- van den Bedem H, Bhabha G, Yang K, Wright P, Fraser J. Automated identification of functional dynamic contact networks from X-ray crystallography. Nat Methods. 2013;10:896-902 pubmed publisher..CONTACT-guided mutagenesis can exploit the structure-dynamics-function relationship in protein engineering and design. ..
- Khor V, Shen W, Kraemer F. Lipid droplet metabolism. Curr Opin Clin Nutr Metab Care. 2013;16:632-7 pubmed publisher..Lipid droplets are dynamically active, responding to changes in cellular physiology, as well as interacting with cytosolic proteins and other organelles to control lipid homeostasis. ..
- Tsai A, Puglisi J, Uemura S. Probing the Translation Dynamics of Ribosomes Using Zero-Mode Waveguides. Prog Mol Biol Transl Sci. 2016;139:1-43 pubmed publisher..Given the success of the technology, similarly complex biological processes could be studied in near-physiological conditions with the controllability of conventional in vitro experiments. ..
- Halenbeck R, Macdonald H, Roulston A, Chen T, Conroy L, Williams L. CPAN, a human nuclease regulated by the caspase-sensitive inhibitor DFF45. Curr Biol. 1998;8:537-40 pubmed..CPAN is a tightly regulated endonuclease with unique characteristics that might represent a distinctive family of endonucleases. ..
- Spencer K, Graus Porta D, Leng J, Hynes N, Klemke R. ErbB2 is necessary for induction of carcinoma cell invasion by ErbB family receptor tyrosine kinases. J Cell Biol. 2000;148:385-97 pubmed..Thus, ErbB2 serves as a critical component that couples ErbB receptor tyrosine kinases to the migration/invasion machinery of carcinoma cells. ..
- Chu Z, Pio F, Xie Z, Welsh K, Krajewska M, Krajewski S, et al. A novel enhancer of the Apaf1 apoptosome involved in cytochrome c-dependent caspase activation and apoptosis. J Biol Chem. 2001;276:9239-45 pubmed..NAC expression in vivo is associated with terminal differentiation of short lived cells in epithelia and some other tissues. The ability of NAC to enhance Apaf1-apoptosome function reveals a novel paradigm for apoptosis regulation. ..
- Chen J, Fang G. MAD2B is an inhibitor of the anaphase-promoting complex. Genes Dev. 2001;15:1765-70 pubmed..Unlike MAD2, whose interaction with MAD1 is required for mitotic checkpoint control, MAD2B does not interact with MAD1, suggesting that MAD2B may relay a different cellular signal to APC. ..
- Prekeris R, Davies J, Scheller R. Identification of a novel Rab11/25 binding domain present in Eferin and Rip proteins. J Biol Chem. 2001;276:38966-70 pubmed..The data suggest that various Rab effectors compete with each other for binding to Rab11/25 possibly accounting for the diversity of Rab11 functions. ..
- Price D, Brooks C. Modern protein force fields behave comparably in molecular dynamics simulations. J Comput Chem. 2002;23:1045-57 pubmed..When all three proteins are considered together, no force field showed any consistent trend in variations for most of the properties monitored in the study. ..
- Tedesco D, Lukas J, Reed S. The pRb-related protein p130 is regulated by phosphorylation-dependent proteolysis via the protein-ubiquitin ligase SCF(Skp2). Genes Dev. 2002;16:2946-57 pubmed..Thus, like the Cdk inhibitor p27(Kip1), p130 turnover is regulated by Cdk-dependent G1 phosphorylation leading to ubiquitin-dependent proteolysis. ..
- Chen J, Im W, Brooks C. Refinement of NMR structures using implicit solvent and advanced sampling techniques. J Am Chem Soc. 2004;126:16038-47 pubmed..This refinement procedure is also expected to be useful when redundant experimental data are not readily available, such as for large multidomain biomolecules and in solid-state NMR structure determination. ..
- Roelink H, Nusse R. Expression of two members of the Wnt family during mouse development--restricted temporal and spatial patterns in the developing neural tube. Genes Dev. 1991;5:381-8 pubmed..Characteristic expression patterns of these two closely related genes suggest that Wnt-3 and Wnt-3A play distinct roles in cell-cell signaling during morphogenesis of the developing neural tube. ..