Genomes and Genes
Experts and Doctors on nih 3t3 cells in New York, United States
Locale: New York, United States
Topic: nih 3t3 cells
Publications146 found, 100 shown here
- Dbouk H, Khalil B, Wu H, Shymanets A, Nurnberg B, Backer J. Characterization of a tumor-associated activating mutation of the p110? PI 3-kinase. PLoS ONE. 2013;8:e63833 pubmed publisher..We propose that the E633K mutant activates p110? by enhancing its basal association with membranes. This study presents the first analysis of an activating oncogenic mutation of p110?. ..
- Benezra M, Hambardzumyan D, Penate Medina O, Veach D, Pillarsetty N, Smith Jones P, et al. Fluorine-labeled dasatinib nanoformulations as targeted molecular imaging probes in a PDGFB-driven murine glioblastoma model. Neoplasia. 2012;14:1132-43 pubmed..18)F-SKI249380 is a PDGFR-selective tracer, which demonstrates improved delivery to PDGFB-driven high-grade gliomas and facilitates treatment planning when coupled with nanoformulations and quantitative PET imaging approaches. ..
- Dabrowska M, Sirotnak F. Regulation of transcription of the human MRP7 gene. Characteristics of the basal promoter and identification of tumor-derived transcripts encoding additional 5' end heterogeneity. Gene. 2004;341:129-39 pubmed..This 5' end heterogeneity infers a more complex intron-exon composition than hitherto shown. ..
- Du Z, Shen Y, Yang W, Mecklenbrauker I, Neel B, Ivashkiv L. Inhibition of IFN-alpha signaling by a PKC- and protein tyrosine phosphatase SHP-2-dependent pathway. Proc Natl Acad Sci U S A. 2005;102:10267-72 pubmed..Together, our data describe a rapidly inducible, direct mechanism of inhibition of Jak-STAT signaling mediated by a PKCbeta-SHP-2 signaling pathway. ..
- Zhao M, Janas J, Niki M, Pandolfi P, Van Aelst L. Dok-1 independently attenuates Ras/mitogen-activated protein kinase and Src/c-myc pathways to inhibit platelet-derived growth factor-induced mitogenesis. Mol Cell Biol. 2006;26:2479-89 pubmed..Our data suggest a mechanistic basis for the inhibitory effect of Dok-1 on growth factor-induced mitogenesis and its role as a tumor suppressor. ..
- Matsuo N, Tanaka S, Yoshioka H, Koch M, Gordon M, Ramirez F. Collagen XXIV (Col24a1) gene expression is a specific marker of osteoblast differentiation and bone formation. Connect Tissue Res. 2008;49:68-75 pubmed publisher..These findings lend further support to the hypothesis that collagen XXIV may be implicated in the formation of a mineralization-competent bone matrix. ..
- Mujtaba S, He Y, Zeng L, Yan S, Plotnikova O, Sachchidanand -, et al. Structural mechanism of the bromodomain of the coactivator CBP in p53 transcriptional activation. Mol Cell. 2004;13:251-63 pubmed..Using structural and biochemical analyses, we define the molecular determinants for the specificity of this molecular recognition. ..
- Snyder M, Huang X, Zhang J. Stat3 is essential for neuronal differentiation through direct transcriptional regulation of the Sox6 gene. FEBS Lett. 2011;585:148-52 pubmed publisher..Moreover, using an RNA interference approach, we show that Stat3 is required for Sox6 expression during neuronal differentiation. ..
- Jimenez M, Perez de Castro I, Benet M, Garcia J, Inghirami G, Pellicer A. The Rgr oncogene induces tumorigenesis in transgenic mice. Cancer Res. 2004;64:6041-9 pubmed..These findings provide new insights into the tumorigenic role of Rgr as a potent oncogene and show that p15INK4b can act as a tumor suppressor gene. ..
- Massey A, Follenzi A, Kiffin R, Zhang C, Cuervo A. Early cellular changes after blockage of chaperone-mediated autophagy. Autophagy. 2008;4:442-56 pubmed..Based on the time-course of the cellular changes, we propose that a minimal threshold of these toxic products needs to accumulate in order to constitutively activate macroautophagy and thus return cellular homeostasis to normal. ..
- Su W, Yeku O, Olepu S, Genna A, Park J, Ren H, et al. 5-Fluoro-2-indolyl des-chlorohalopemide (FIPI), a phospholipase D pharmacological inhibitor that alters cell spreading and inhibits chemotaxis. Mol Pharmacol. 2009;75:437-46 pubmed publisher..However, FIPI does inhibit PLD regulation of F-actin cytoskeleton reorganization, cell spreading, and chemotaxis, indicating potential utility for it as a therapeutic for autoimmunity and cancer metastasis. ..
- Shen J, Vil M, Prewett M, Damoci C, Zhang H, Li H, et al. Development of a fully human anti-PDGFRbeta antibody that suppresses growth of human tumor xenografts and enhances antitumor activity of an anti-VEGFR2 antibody. Neoplasia. 2009;11:594-604 pubmed..Taken together, these results lend great support to the use of PDGFRbeta antagonists in combination with other antiangiogenic agents in the treatment of a broad range of human cancers. ..
- Liberatore R, Bieniasz P. Tetherin is a key effector of the antiretroviral activity of type I interferon in vitro and in vivo. Proc Natl Acad Sci U S A. 2011;108:18097-101 pubmed publisher..These data indicate that tetherin is a bona fide antiviral protein and can reduce retroviral replication and disease in vivo. ..
- Zammarchi F, Boutsalis G, Cartegni L. 5' UTR control of native ERG and of Tmprss2:ERG variants activity in prostate cancer. PLoS ONE. 2013;8:e49721 pubmed publisher..Usage of this ATG can be preferentially down-regulated by directed antisense-based compounds, possibly representing the basis of a targeted approach that distinguishes between tumor-associated and normal ERG. ..
- Kim J, Seandel M, Falciatori I, Wen D, Rafii S. CD34+ testicular stromal cells support long-term expansion of embryonic and adult stem and progenitor cells. Stem Cells. 2008;26:2516-22 pubmed publisher..Disclosure of potential conflicts of interest is found at the end of this article. ..
- Ching G, Liem R. RE1 silencing transcription factor is involved in regulating neuron-specific expression of alpha-internexin and neurofilament genes. J Neurochem. 2009;109:1610-23 pubmed publisher..Thus, REST regulates expression of these neuronal genes, partly by a HDAC-dependent epigenetic mechanism. ..
- Benet M, Dulman R, Suzme R, de Miera E, Vega M, Nguyen T, et al. Wild type N-ras displays anti-malignant properties, in part by downregulating decorin. J Cell Physiol. 2012;227:2341-51 pubmed publisher..The components of the pathways modulated by wt N-ras mediating its anti-malignant effects are potential targets for therapeutic intervention in cancer. ..
- Anguiano J, Garner T, Mahalingam M, Das B, Gavathiotis E, Cuervo A. Chemical modulation of chaperone-mediated autophagy by retinoic acid derivatives. Nat Chem Biol. 2013;9:374-82 pubmed publisher..We demonstrate that chemical enhancement of CMA protects cells from oxidative stress and from proteotoxicity, supporting a potential therapeutic opportunity when reduced CMA contributes to cellular dysfunction and disease. ..
- Rivas S, ArmisÃ©n R, Rojas D, Maldonado E, Huerta H, Tapia J, et al. The Ski Protein is Involved in the Transformation Pathway of Aurora Kinase A. J Cell Biochem. 2016;117:334-43 pubmed publisher..Altogether, these results suggest that the Ski protein is a target in the transformation pathway mediated by the AURKA oncogene. ..
- David C, Chen M, Assanah M, Canoll P, Manley J. HnRNP proteins controlled by c-Myc deregulate pyruvate kinase mRNA splicing in cancer. Nature. 2010;463:364-8 pubmed publisher..Our results thus define a pathway that regulates an alternative splicing event required for tumour cell proliferation. ..
- Stoffel A, Chaurushiya M, Singh B, Levine A. Activation of NF-kappaB and inhibition of p53-mediated apoptosis by API2/mucosa-associated lymphoid tissue 1 fusions promote oncogenesis. Proc Natl Acad Sci U S A. 2004;101:9079-84 pubmed..Furthermore, they provide evidence underlying the emerging role of the NF-kappaB signaling pathway in the inhibition of apoptosis. ..
- Schlesinger S, Goff S. Silencing of proviruses in embryonic cells: efficiency, stability and chromatin modifications. EMBO Rep. 2013;14:73-9 pubmed publisher..The results indicate that epigenetic mechanisms with different specificity and efficiency are used to silence the exogenous retroviral sequences in embryonic cells...
- Lee J, Hayman E, Pegram H, Santos E, Heller G, Sadelain M, et al. In vivo inhibition of human CD19-targeted effector T cells by natural T regulatory cells in a xenotransplant murine model of B cell malignancy. Cancer Res. 2011;71:2871-81 pubmed publisher..These findings have significant implications for the design of future clinical trials utilizing CAR-based adoptive T-cell therapies of cancer. ..
- Leslie K, Lang C, Devgan G, Azare J, Berishaj M, Gerald W, et al. Cyclin D1 is transcriptionally regulated by and required for transformation by activated signal transducer and activator of transcription 3. Cancer Res. 2006;66:2544-52 pubmed..These results suggest both a cdk-dependent and cdk-independent role for cyclin D1 in modulating transformation by different oncogenes. ..
- Folker E, Ostlund C, Luxton G, Worman H, Gundersen G. Lamin A variants that cause striated muscle disease are defective in anchoring transmembrane actin-associated nuclear lines for nuclear movement. Proc Natl Acad Sci U S A. 2011;108:131-6 pubmed publisher..Nesprin-2G TAN lines also slipped in SUN2-depleted cells. Our results establish A-type lamins as anchors for nesprin-2G-SUN2 TAN lines to allow productive movement and proper positioning of the nucleus by actin...
- Likar Y, Zurita J, Dobrenkov K, Shenker L, Cai S, Neschadim A, et al. A new pyrimidine-specific reporter gene: a mutated human deoxycytidine kinase suitable for PET during treatment with acycloguanosine-based cytotoxic drugs. J Nucl Med. 2010;51:1395-403 pubmed publisher..Additionally, the prosuicidal activity of these reporters with pyrimidine-based analogs will allow for the safe elimination of transduced cells. ..
- Bejarano E, Girao H, Yuste A, Patel B, Marques C, Spray D, et al. Autophagy modulates dynamics of connexins at the plasma membrane in a ubiquitin-dependent manner. Mol Biol Cell. 2012;23:2156-69 pubmed publisher..This study reveals a novel regulatory role for macroautophagy in GJ function that is directly dependent on the ubiquitinylation of plasma membrane connexins. ..
- Persaud K, Tille J, Liu M, Zhu Z, Jimenez X, Pereira D, et al. Involvement of the VEGF receptor 3 in tubular morphogenesis demonstrated with a human anti-human VEGFR-3 monoclonal antibody that antagonizes receptor activation by VEGF-C. J Cell Sci. 2004;117:2745-56 pubmed..Similar results were obtained with HUVECs. Together, these findings point to a role for VEGFR-3 in vascular tubular morphogenesis and highlight the utility of hF4-3C5 as a tool for the investigation of the biology of VEGFR-3. ..
- Shih A, Dai C, Hu X, Rosenblum M, Koutcher J, Holland E. Dose-dependent effects of platelet-derived growth factor-B on glial tumorigenesis. Cancer Res. 2004;64:4783-9 pubmed..Our data show that PDGFR signaling quantitatively regulates tumor grade and is required to sustain high-grade oligodendrogliomas. ..
- Xiao A, Li H, Shechter D, Ahn S, Fabrizio L, Erdjument Bromage H, et al. WSTF regulates the H2A.X DNA damage response via a novel tyrosine kinase activity. Nature. 2009;457:57-62 pubmed publisher..Our work demonstrates a new mechanism that regulates the DNA damage response and expands our knowledge of domains that contain intrinsic tyrosine kinase activity. ..
- Rosenfeld A. Suppression of cellular transformation by poly (A) binding protein interacting protein 2 (Paip2). PLoS ONE. 2011;6:e25116 pubmed publisher..The ability of Paip2 to bind to Pabp is required to suppress the transformed phenotype mediated by hRas(V12). These observations indicate that Paip2 is able to function as a tumor suppressor. ..
- Lewis T, Prywes R. Serum regulation of Id1 expression by a BMP pathway and BMP responsive element. Biochim Biophys Acta. 2013;1829:1147-59 pubmed publisher..Given these and other findings we conclude that the Id family of IEGs is regulated by BMPs in serum through similar BREs. This represents a second pathway for serum regulation of IEGs. ..
- Aguilo F, Li S, Balasubramaniyan N, Sancho A, Benko S, Zhang F, et al. Deposition of 5-Methylcytosine on Enhancer RNAs Enables the Coactivator Function of PGC-1α. Cell Rep. 2016;14:479-492 pubmed publisher..Enrichment of m(5)C within eRNA species coincides with metabolic stress of fasting in vivo. Collectively, these findings illustrate the complex epigenetic circuitry imposed by PGC-1α at the eRNA level to fine-tune energy metabolism. ..
- Wang X, Lee H, Capozza F, Marmon S, Sotgia F, Brooks J, et al. Tyrosine phosphorylation of caveolin-2 at residue 27: differences in the spatial and temporal behavior of phospho-Cav-2 (pY19 and pY27). Biochemistry. 2004;43:13694-706 pubmed..Taken together, these results suggest that, in addition to the common characteristics that these two sites appear to share, phospho-Cav-2 (pY19) and phospho-Cav-2 (pY27) may each possess a set of unique functional roles. ..
- Talos F, Petrenko O, Mena P, Moll U. Mitochondrially targeted p53 has tumor suppressor activities in vivo. Cancer Res. 2005;65:9971-81 pubmed..Our in vivo data on the direct mitochondrial apoptotic p53 program lays the groundwork to further investigate its efficacy and safety and to address its possible therapeutic value in the future. ..
- Breda L, Casu C, Gardenghi S, Bianchi N, Cartegni L, NARLA M, et al. Therapeutic hemoglobin levels after gene transfer in ?-thalassemia mice and in hematopoietic cells of ?-thalassemia and sickle cells disease patients. PLoS ONE. 2012;7:e32345 pubmed publisher..This approach would provide vital information to select the best candidates for these clinical trials, before patients undergo myeloablation and bone marrow transplant. ..
- Mason J, Morrison D, Bassit B, Dimri M, Band H, Licht J, et al. Tyrosine phosphorylation of Sprouty proteins regulates their ability to inhibit growth factor signaling: a dual feedback loop. Mol Biol Cell. 2004;15:2176-88 pubmed..Thus, these data highlight a novel negative and positive regulatory loop that allows for the controlled, homeostatic inhibition of RTK signaling. ..
- Bernardi R, Scaglioni P, Bergmann S, Horn H, Vousden K, Pandolfi P. PML regulates p53 stability by sequestering Mdm2 to the nucleolus. Nat Cell Biol. 2004;6:665-72 pubmed..These findings demonstrate an unexpected role of PML in the nucleolar network for tumour suppression. ..
- Ezratty E, Partridge M, Gundersen G. Microtubule-induced focal adhesion disassembly is mediated by dynamin and focal adhesion kinase. Nat Cell Biol. 2005;7:581-90 pubmed..Inhibition of dynamin prevents migration of cells with a focal adhesion phenotype. Our results show that focal adhesion disassembly involves microtubules, dynamin and FAK, and is not simply the reversal of focal adhesion formation. ..
- Bergamin E, Hallock P, Burden S, Hubbard S. The cytoplasmic adaptor protein Dok7 activates the receptor tyrosine kinase MuSK via dimerization. Mol Cell. 2010;39:100-9 pubmed publisher..The structure provides the molecular basis for MuSK activation by Dok7 and for rationalizing several Dok7 loss-of-function mutations found in patients with congenital myasthenic syndromes. ..
- Xiong J, Fan S, Meng Q, Schramm L, Wang C, Bouzahza B, et al. BRCA1 inhibition of telomerase activity in cultured cells. Mol Cell Biol. 2003;23:8668-90 pubmed..Finally, endogenous BRCA1 and c-Myc were detected on transfected mouse and human TERT promoter segments in vivo. We postulate that inhibition of telomerase may contribute to the BRCA1 tumor suppressor activity. ..
- Evans K, Gomes E, Reisenweber S, Gundersen G, Lauring B. Linking axonal degeneration to microtubule remodeling by Spastin-mediated microtubule severing. J Cell Biol. 2005;168:599-606 pubmed..Using purified components, we also show that Spastin interacts directly with microtubules and is sufficient for severing. These studies suggest that defects in microtubule severing are a cause of axonal degeneration in human disease. ..
- Vethantham V, Rao N, Manley J. Sumoylation modulates the assembly and activity of the pre-mRNA 3' processing complex. Mol Cell Biol. 2007;27:8848-58 pubmed
- Yasuda Y, Li Z, Greenbaum D, Bogyo M, Weber E, Bromme D. Cathepsin V, a novel and potent elastolytic activity expressed in activated macrophages. J Biol Chem. 2004;279:36761-70 pubmed..Because the GAG content is reduced in atherosclerotic plaques, an increase of cathepsins V and K activities may accelerate the destruction of the elastin matrix in diseased arteries. ..
- Perez de Castro I, Benet M, Jimenez M, Alzabin S, Malumbres M, Pellicer A. Mouse p10, an alternative spliced form of p15INK4b, inhibits cell cycle progression and malignant transformation. Cancer Res. 2005;65:3249-56 pubmed
- Donepudi M, Resh M. c-Src trafficking and co-localization with the EGF receptor promotes EGF ligand-independent EGF receptor activation and signaling. Cell Signal. 2008;20:1359-67 pubmed publisher..These data suggest that the synergy between c-Src and EGFR also occurs as these two kinases traffic together, and that their co-localization promotes EGFR-mediated signaling. ..
- Chyou S, Ekland E, Carpenter A, Tzeng T, Tian S, Michaud M, et al. Fibroblast-type reticular stromal cells regulate the lymph node vasculature. J Immunol. 2008;181:3887-96 pubmed
- Dubeykovskaya Z, Dubeykovskiy A, Solal Cohen J, Wang T. Secreted trefoil factor 2 activates the CXCR4 receptor in epithelial and lymphocytic cancer cell lines. J Biol Chem. 2009;284:3650-62 pubmed publisher..Overall these data identify CXCR4 as a bona fide signaling receptor for TFF2 and suggest a mechanism through which TFF2 may modulate immune and tumorigenic responses in vivo. ..
- Shen J, Snapp E, Lippincott Schwartz J, Prywes R. Stable binding of ATF6 to BiP in the endoplasmic reticulum stress response. Mol Cell Biol. 2005;25:921-32 pubmed..We propose that stable BiP binding is essential for ATF6 regulation and that ER stress dissociates BiP from ATF6 by actively restarting the BiP ATPase cycle. ..
- Krivtsov A, Rozov F, Zinovyeva M, Hendrikx P, Jiang Y, Visser J, et al. Jedi--a novel transmembrane protein expressed in early hematopoietic cells. J Cell Biochem. 2007;101:767-84 pubmed..On the basis of obtained results, we suggest that Jedi is involved in the fine regulation of the early stages of hematopoietic differentiation, presumably through the Notch signaling pathway. ..
- Spektor A, Tsang W, Khoo D, Dynlacht B. Cep97 and CP110 suppress a cilia assembly program. Cell. 2007;130:678-90 pubmed..Identification of Cep97 and other genes involved in regulation of cilia assembly may accelerate our understanding of human ciliary diseases, including renal disease and retinal degeneration. ..
- Cai C, Peng Y, Buckley M, Wei J, Chen F, Liebes L, et al. Epidermal growth factor receptor activation in prostate cancer by three novel missense mutations. Oncogene. 2008;27:3201-10 pubmed publisher..Molecules that regulate the mechanisms of their oncogenic activation represent novel targets for limiting tumor cell progression, and further elucidation of these mutations will have utility in prostate cancer treatment. ..
- Schlesinger S, Lee A, Wang G, Green L, Goff S. Proviral silencing in embryonic cells is regulated by Yin Yang 1. Cell Rep. 2013;4:50-8 pubmed publisher..Finally, we report an embryonic cell-specific interaction between YY1 and Trim28. Our results suggest a major role for YY1 in the silencing of both exogenous retroviruses and ERVs in embryonic cells. ..
- Li H, Okamoto K, Peart M, Prives C. Lysine-independent turnover of cyclin G1 can be stabilized by B'alpha subunits of protein phosphatase 2A. Mol Cell Biol. 2009;29:919-28 pubmed publisher..Our results thus indicate that proteasomal turnover of cyclin G1 is regulated by noncanonical processes. ..
- Soccio R, Adams R, Maxwell K, Breslow J. Differential gene regulation of StarD4 and StarD5 cholesterol transfer proteins. Activation of StarD4 by sterol regulatory element-binding protein-2 and StarD5 by endoplasmic reticulum stress. J Biol Chem. 2005;280:19410-8 pubmed..Because StarD4 is regulated by sterols via SREBP-2, whereas StarD5 is activated by ER stress, they likely serve distinct functions in cholesterol metabolism. ..
- Goodchild R, Dauer W. The AAA+ protein torsinA interacts with a conserved domain present in LAP1 and a novel ER protein. J Cell Biol. 2005;168:855-62 pubmed
- Chen Z, Torrens J, Anand A, Spiegelman B, Friedman J. Krox20 stimulates adipogenesis via C/EBPbeta-dependent and -independent mechanisms. Cell Metab. 2005;1:93-106 pubmed..These data indicate that Krox20 is necessary for adipogenesis and that, when overexpressed, Krox20 potently stimulates adipogenesis via C/EBPbeta-dependent and -independent mechanisms. ..
- Feng R, Desbordes S, Xie H, Tillo E, Pixley F, Stanley E, et al. PU.1 and C/EBPalpha/beta convert fibroblasts into macrophage-like cells. Proc Natl Acad Sci U S A. 2008;105:6057-62 pubmed publisher..Our data suggest that it might become possible to induce the transdifferentiation of skin-derived fibroblasts into cell types desirable for tissue regeneration. ..
- Tian W, Liou H. RNAi-mediated c-Rel silencing leads to apoptosis of B cell tumor cells and suppresses antigenic immune response in vivo. PLoS ONE. 2009;4:e5028 pubmed publisher..Our study thus validates the efficacy of c-Rel-siRNA, and suggests the development of siRNA-based therapy, as well as small molecular inhibitors for the treatment of B cell tumors as well as autoimmune diseases. ..
- Gauthier N, Rossier O, Mathur A, Hone J, Sheetz M. Plasma membrane area increases with spread area by exocytosis of a GPI-anchored protein compartment. Mol Biol Cell. 2009;20:3261-72 pubmed publisher..Therefore, we suggest that fibroblasts are able to regulate about half of their original PM area by the addition of membrane via a glycosylphosphatidylinositol-anchored protein compartment. ..
- Sashida G, Bae N, Di Giandomenico S, Asai T, Gurvich N, Bazzoli E, et al. The mef/elf4 transcription factor fine tunes the DNA damage response. Cancer Res. 2011;71:4857-65 pubmed publisher..Thus, although ELF4 promotes the phosphorylation of H2AX by ATM, its activity must be dampened by ATM-dependent phosphorylation and degradation to avoid an excessive DNA damage response. ..
- Alarcon Vargas D, RONAI Z. c-Jun-NH2 kinase (JNK) contributes to the regulation of c-Myc protein stability. J Biol Chem. 2004;279:5008-16 pubmed..In all, these findings point to the contribution of JNK to the regulation of c-Myc protein stability under normal growth conditions. ..
- Ford B, Skowronek K, Boykevisch S, Bar Sagi D, Nassar N. Structure of the G60A mutant of Ras: implications for the dominant negative effect. J Biol Chem. 2005;280:25697-705 pubmed..Our data suggest that the dominant negative effect observed for RasG60A.GTP could result from the sequestering of Sos in a non-productive Ras-GTP-guanine nucleotide exchange factor ternary complex. ..
- Wang D, Tan Y, Kreitzer G, Nakai Y, Shan D, Zheng Y, et al. G proteins G12 and G13 control the dynamic turnover of growth factor-induced dorsal ruffles. J Biol Chem. 2006;281:32660-7 pubmed..Thus, Galpha(12)/Galpha(13) critically regulate dorsal ruffle turnover. ..
- Schmoranzer J, Fawcett J, Segura M, Tan S, Vallee R, Pawson T, et al. Par3 and dynein associate to regulate local microtubule dynamics and centrosome orientation during migration. Curr Biol. 2009;19:1065-74 pubmed publisher..We propose that Par3 acts as a cortical factor that tethers MTs through its association with LIC2 dynein. ..
- Dujardin D, Barnhart L, Stehman S, Gomes E, Gundersen G, Vallee R. A role for cytoplasmic dynein and LIS1 in directed cell movement. J Cell Biol. 2003;163:1205-11 pubmed..Together, these results implicate a leading edge cortical pool of dynein in both early and persistent steps in directed cell movement. ..
- Wen Y, Eng C, Schmoranzer J, Cabrera Poch N, Morris E, Chen M, et al. EB1 and APC bind to mDia to stabilize microtubules downstream of Rho and promote cell migration. Nat Cell Biol. 2004;6:820-30 pubmed..These results show an evolutionarily conserved pathway for microtubule capture, and suggest that mDia functions as a scaffold protein for EB1 and APC to stabilize microtubules and promote cell migration. ..
- Goll M, Kirpekar F, Maggert K, Yoder J, Hsieh C, Zhang X, et al. Methylation of tRNAAsp by the DNA methyltransferase homolog Dnmt2. Science. 2006;311:395-8 pubmed..Indirect sequence recognition is also a feature of eukaryotic DNA methyltransferases, which may have arisen from a Dnmt2-like RNA methyltransferase. ..
- Subbaramaiah K, Howe L, Port E, Brogi E, Fishman J, Liu C, et al. HER-2/neu status is a determinant of mammary aromatase activity in vivo: evidence for a cyclooxygenase-2-dependent mechanism. Cancer Res. 2006;66:5504-11 pubmed..Collectively, our data indicate that COX-2 is the functional intermediate linking HER-2/neu and aromatase and suggest that inhibitors of PGE(2) synthesis will suppress estrogen biosynthesis in breast tissue. ..
- Eng C, Huckaba T, Gundersen G. The formin mDia regulates GSK3beta through novel PKCs to promote microtubule stabilization but not MTOC reorientation in migrating fibroblasts. Mol Biol Cell. 2006;17:5004-16 pubmed..Novel PKCs also interacted with mDia in vivo and in vitro. These results identify a new activity for the formin mDia in regulating GSK3beta through novel PKCs and implicate novel PKCs as new factors in the MT stabilization pathway. ..
- Busino L, Bassermann F, Maiolica A, Lee C, Nolan P, Godinho S, et al. SCFFbxl3 controls the oscillation of the circadian clock by directing the degradation of cryptochrome proteins. Science. 2007;316:900-4 pubmed..Silencing of Fbxl3 produced no effect in Cry1-/-;Cry2-/- cells, which shows that Fbxl3 controls clock oscillations by mediating the degradation of CRY proteins. ..
- Bartolini F, Moseley J, Schmoranzer J, Cassimeris L, Goode B, Gundersen G. The formin mDia2 stabilizes microtubules independently of its actin nucleation activity. J Cell Biol. 2008;181:523-36 pubmed publisher..These results indicate that mDia2 has a novel MT stabilization activity that is separate from its actin nucleation activity. ..
- Lotti F, Imlach W, Saieva L, Beck E, Hao L, Li D, et al. An SMN-dependent U12 splicing event essential for motor circuit function. Cell. 2012;151:440-54 pubmed publisher..These findings directly link defective splicing of critical neuronal genes induced by SMN deficiency to motor circuit dysfunction, establishing a molecular framework for the selective pathology of SMA. ..
- Tung C, Han M, Qi J. Total control of fat cells from adipogenesis to apoptosis using a xanthene analog. PLoS ONE. 2017;12:e0179158 pubmed publisher..These dual functionalities make MI-401 an effective agent in the regulation of the birth and death of adipocytes. ..
- Selvaraj A, Prywes R. Expression profiling of serum inducible genes identifies a subset of SRF target genes that are MKL dependent. BMC Mol Biol. 2004;5:13 pubmed..MKL may also contribute to the induction of other SRF target genes however its role is not essential, possibly due to other activation mechanisms such as MAPK phosphorylation of TCFs. ..
- Kuo T, Calame K. B lymphocyte-induced maturation protein (Blimp)-1, IFN regulatory factor (IRF)-1, and IRF-2 can bind to the same regulatory sites. J Immunol. 2004;173:5556-63 pubmed..Thus, our data suggest that Blimp-1 competes in vivo with a subset of IRF proteins and help predict the sites and IRF family members that may be affected. ..
- Kentsis A, Topisirovic I, Culjkovic B, Shao L, Borden K. Ribavirin suppresses eIF4E-mediated oncogenic transformation by physical mimicry of the 7-methyl guanosine mRNA cap. Proc Natl Acad Sci U S A. 2004;101:18105-10 pubmed..In all, ribavirin's association with eIF4E may provide a pharmacologic means for the interruption of posttranscriptional networks of oncogenes that maintain and enhance neoplasia and malignancy in human cancer. ..
- Gomes E, Jani S, Gundersen G. Nuclear movement regulated by Cdc42, MRCK, myosin, and actin flow establishes MTOC polarization in migrating cells. Cell. 2005;121:451-63 pubmed..These results show that nuclear repositioning is an initial polarizing event in migrating cells and that the positions of the nucleus and the MTOC are established by separate regulatory pathways. ..
- Sarkaria I, O charoenrat P, Talbot S, Reddy P, Ngai I, Maghami E, et al. Squamous cell carcinoma related oncogene/DCUN1D1 is highly conserved and activated by amplification in squamous cell carcinomas. Cancer Res. 2006;66:9437-44 pubmed..Collectively, these data suggest that SCCRO is a novel component of the HH signaling pathway involved in the malignant transformation of squamous cell lineage. ..
- Mancia F, Brenner Morton S, Siegel R, Assur Z, Sun Y, Schieren I, et al. Production and characterization of monoclonal antibodies sensitive to conformation in the 5HT2c serotonin receptor. Proc Natl Acad Sci U S A. 2007;104:4303-8 pubmed..Five of the nine mAbs have cytoplasmic epitopes, and two of these are sensitive to the ligand state of the receptor. These properties should be useful both for structural analysis and in probes of function. ..
- Hoshino A, Fujii H. Nuclear translocation of 2-amino-3-ketobutyrate coenzyme A ligase by cold and osmotic stress. Cell Stress Chaperones. 2007;12:186-91 pubmed..A large region of KBL was required for stress-induced nuclear translocation. The KBL reporter system will be a useful tool for the investigation of cold and osmotic stress responses. ..