Genomes and Genes
Experts and Doctors on molecular sequence data in Scotland, United Kingdom
Locale: Scotland, United Kingdom
Topic: molecular sequence data
Publications377 found, 100 shown here
- McMahon S, Oke M, Liu H, Johnson K, Carter L, Kadi N, et al. Purification, crystallization and data collection of Pectobacterium chrysanthemi AcsD, a type A siderophore synthetase. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2008;64:1052-5 pubmed publisher..The protein has no convincing sequence match to any known structure and thus solution is likely to require experimental phasing...
- Gibriel A, Tate R, Yu Y, Rawson Lax E, Hammer H, Tettey J, et al. The p.Arg86Gln change in GARP2 (glutamic acid-rich protein-2) is a common West African-related polymorphism. Gene. 2013;515:155-8 pubmed publisher..The p.Arg86Gln mutation actually appears to be a polymorphism common in ethnic West Africans and not associated with RP. This change may provide a useful marker for West African ancestry. ..
- Kaiser K, Goodwin S. "Site-selected" transposon mutagenesis of Drosophila. Proc Natl Acad Sci U S A. 1990;87:1686-90 pubmed..Phenotypic criteria were used only retrospectively to verify our diagnoses. There are obvious implications of this technique for the mutagenesis of other organisms. ..
- McKay S, Thomson E, Cooke H. Sequence homologies and linkage group conservation of the human and mouse Cenpc genes. Genomics. 1994;22:36-40 pubmed..Secondary sites are present in man on chromosome 12q21.2-q21.33 and in mouse on chromosome 2B. This mouse secondary site is a pseudogene on the basis of DNA sequence. These secondary sites are not syntenic in the two species. ..
- Cook J, McGregor A, Lee T, Milligan G, Eidne K. A disulfide bonding interaction role for cysteines in the extracellular domain of the thyrotropin-releasing hormone receptor. Endocrinology. 1996;137:2851-8 pubmed..Cys100 is not thought to have a disulfide bonding interaction role. Results obtained after chemical modification have shown that free sulfhydryl groups within the TRH-R may also have a role in ligand interactions. ..
- Thompson F, Martin S, Devaney E. Brugia pahangi: characterisation of a small heat shock protein cDNA clone. Exp Parasitol. 1996;83:259-66 pubmed..The expression of the small heat shock protein cDNA appears to be controlled at the level of RNA synthesis and is highly stage specific and temperature dependent. ..
- Hawley S, Davison M, Woods A, Davies S, Beri R, Carling D, et al. Characterization of the AMP-activated protein kinase kinase from rat liver and identification of threonine 172 as the major site at which it phosphorylates AMP-activated protein kinase. J Biol Chem. 1996;271:27879-87 pubmed..A., Selbert, M. A., Goldstein, E. G., Edelman, A. M., Carling, D., and Hardie, D. G. (1995) J. Biol. Chem. 270, 27186-27191). ..
- Spence S, Rena G, Sullivan M, Erdogan S, Houslay M. Receptor-mediated stimulation of lipid signalling pathways in CHO cells elicits the rapid transient induction of the PDE1B isoform of Ca2+/calmodulin-stimulated cAMP phosphodiesterase. Biochem J. 1997;321 ( Pt 1):157-63 pubmed
- Berriman M, Fairlamb A. Detailed characterization of a cyclophilin from the human malaria parasite Plasmodium falciparum. Biochem J. 1998;334 ( Pt 2):437-45 pubmed..We propose that a malarial CyP-CsA complex presents residues 3 and 4 as part of an 'effector surface' for recognition by a downstream target, similar to the proposed mechanism for T-cell immunosuppression. ..
- Rhind S, Hopkins J, Dutia B. Amino-terminal sequencing of sheep CD1 antigens and identification of a sheep CD1D gene. Immunogenetics. 1999;49:225-30 pubmed..The predicted amino acid sequence of this gene shows that it is not identical to the SBU-T6 peptide, indicating that a different, CD1D-like gene was cloned. ..
- Jordanides N, Eskdale J, Stuart R, Gallagher G. Allele associations reveal four prominent haplotypes at the human interleukin-6 (IL-6) locus. Genes Immun. 2000;1:451-5 pubmed..We propose that these haplotypes be identified as IL6.0103, IL6.0204, IL6.0207 and IL6.0307, in recognition of their frequency in this population and the alleles that they contain. ..
- Lizcano J, Deak M, Morrice N, Kieloch A, Hastie C, Dong L, et al. Molecular basis for the substrate specificity of NIMA-related kinase-6 (NEK6). Evidence that NEK6 does not phosphorylate the hydrophobic motif of ribosomal S6 protein kinase and serum- and glucocorticoid-induced protein kinase in vivo. J Biol Chem. 2002;277:27839-49 pubmed
- He B, Paterson R, Stock N, Durbin J, Durbin R, Goodbourn S, et al. Recovery of paramyxovirus simian virus 5 with a V protein lacking the conserved cysteine-rich domain: the multifunctional V protein blocks both interferon-beta induction and interferon signaling. Virology. 2002;303:15-32 pubmed..Thus, SV5 blocks two distinct pathways of the innate immune response, both of which require the presence of the C-terminal specific cysteine-rich domain of the multifunctional SV5 V protein. ..
- Dornan J, Taylor P, Walkinshaw M. Structures of immunophilins and their ligand complexes. Curr Top Med Chem. 2003;3:1392-409 pubmed..For all structures discussed in the review a summary of the available biological data is also presented. ..
- Lohkamp B, McDermott G, Campbell S, Coggins J, Lapthorn A. The structure of Escherichia coli ATP-phosphoribosyltransferase: identification of substrate binding sites and mode of AMP inhibition. J Mol Biol. 2004;336:131-44 pubmed..The role of oligomerisation in inhibition and the structural basis for the synergistic inhibition by histidine and AMP are discussed. ..
- Wilson L, Reyns G, Darras V, Coughtrie M. cDNA cloning, functional expression, and characterization of chicken sulfotransferases belonging to the SULT1B and SULT1C families. Arch Biochem Biophys. 2004;428:64-72 pubmed..This is the first detailed characterization of sulfotransferases from the chicken, and it demonstrates that the avian and mammalian SULT1 enzymes are closely related in both structure and function. ..
- Jowsey P, Doherty A, Rouse J. Human PTIP facilitates ATM-mediated activation of p53 and promotes cellular resistance to ionizing radiation. J Biol Chem. 2004;279:55562-9 pubmed..Lowering hPTIP levels also increased cellular sensitivity to IR, suggesting that this protein plays a critical role in maintaining genome stability. ..
- Auld G, Campbell D, Morrice N, Cohen P. Identification of calcium-regulated heat-stable protein of 24 kDa (CRHSP24) as a physiological substrate for PKB and RSK using KESTREL. Biochem J. 2005;389:775-83 pubmed
- Huston E, Lynch M, Mohamed A, Collins D, Hill E, MacLeod R, et al. EPAC and PKA allow cAMP dual control over DNA-PK nuclear translocation. Proc Natl Acad Sci U S A. 2008;105:12791-6 pubmed publisher..Through this signaling system EPAC activation can thereby impact on the Ser-473 phosphorylation status of PKB/Akt and the repair of etoposide-induced DSBs. ..
- Semple F, Webb S, Li H, Patel H, Perretti M, Jackson I, et al. Human beta-defensin 3 has immunosuppressive activity in vitro and in vivo. Eur J Immunol. 2010;40:1073-8 pubmed publisher..This implies a dual role for hBD3 in antimicrobial activity and resolution of inflammation. ..
- Kelly B, Gillespie C, Sherman D, Brophy P. Schwann cells of the myelin-forming phenotype express neurofilament protein NF-M. J Cell Biol. 1992;118:397-410 pubmed..These data demonstrate that Schwann cells remodel their IFs by expressing NF-M before acquiring the myelin-forming phenotype and that IF proteins of the neurofilament-type are not restricted to neurons in the vertebrate nervous system. ..
- Hubbard M, Cohen P. Regulation of protein phosphatase-1G from rabbit skeletal muscle. 1. Phosphorylation by cAMP-dependent protein kinase at site 2 releases catalytic subunit from the glycogen-bound holoenzyme. Eur J Biochem. 1989;186:701-9 pubmed
- Anton I, Coggins J. Sequencing and overexpression of the Escherichia coli aroE gene encoding shikimate dehydrogenase. Biochem J. 1988;249:319-26 pubmed..E. coli shikimate dehydrogenase is homologous to the shikimate dehydrogenase domain of the fungal arom multifunctional enzymes and to the catabolic quinate dehydrogenase of Neurospora crassa. ..
- Bickmore W, Longbottom D, Oghene K, Fletcher J, van Heyningen V. Colocalization of the human CD59 gene to 11p13 with the MIC11 cell surface antigen. Genomics. 1993;17:129-35 pubmed..This localization and the results of immunoprecipitation experiments suggest that the CD59 gene could encode the MIC11 antigen; alternatively this region of 11p13 may contain a cluster of genes encoding cell surface molecules. ..
- Davies R, Roberts A, Morris A, Griffith G, Jerecic J, Ghandi S, et al. Enhanced access to rare brain cDNAs by prescreening libraries: 207 new mouse brain ESTs. Genomics. 1994;24:456-63 pubmed..In total, the comparative sequencing experiments generated 207 new mouse and 11 new rat brain ESTs. ..
- Hall P, Kearsey J, Coates P, Norman D, Warbrick E, Cox L. Characterisation of the interaction between PCNA and Gadd45. Oncogene. 1995;10:2427-33 pubmed..These data provide support for the notion that PCNA-Gadd45 interactions co-ordinate cell cycle and DNA repair. ..
- Snaith H, Armstrong C, Guo Y, Kaiser K, Cohen P. Deficiency of protein phosphatase 2A uncouples the nuclear and centrosome cycles and prevents attachment of microtubules to the kinetochore in Drosophila microtubule star (mts) embryos. J Cell Sci. 1996;109 ( Pt 13):3001-12 pubmed
- Roberts H, Sternberg J, Chappell L. Characterization of calcineurin from Hymenolepis microstoma and H. diminuta and its interaction with cyclosporin A. Parasitology. 1997;114 ( Pt 3):279-83 pubmed..microstoma cyclophilin/CsA. These data suggest the anthelmintic mode of action of CsA in these helminth models does not involve the inhibition of a signal transduction pathway requiring interaction with calcineurin...
- Sargent F, Ballantine S, Rugman P, Palmer T, Boxer D. Reassignment of the gene encoding the Escherichia coli hydrogenase 2 small subunit--identification of a soluble precursor of the small subunit in a hypB mutant. Eur J Biochem. 1998;255:746-54 pubmed
- McSharry C, Xia Y, Holland C, Kennedy M. Natural immunity to Ascaris lumbricoides associated with immunoglobulin E antibody to ABA-1 allergen and inflammation indicators in children. Infect Immun. 1999;67:484-9 pubmed..IgE antibody responses in conjunction with innate inflammatory processes therefore appear to associate with natural immunity to ascariasis. ..
- Zhu H, Alexeev D, Hunter D, Campopiano D, Sadler P. Oxo-iron clusters in a bacterial iron-trafficking protein: new roles for a conserved motif. Biochem J. 2003;376:35-41 pubmed publisher..These findings have implications for understanding bacterial iron acquisition and dissimilation, and organic/mineral interfaces...
- Gare D, Boyd J, Connolly B. Developmental regulation and secretion of nematode-specific cysteine-glycine domain proteins in Trichinella spiralis. Mol Biochem Parasitol. 2004;134:257-66 pubmed..Both predicted proteins contain an N-terminal signal peptide and we subsequently confirmed by MALDI-TOF mass spectrometric analyses of excretory/secretory peptide spots excised from two-dimensional gels that Ts-CCG-2 is secreted. ..
- Zheng X, Tocher D, Dickson C, Bell J, Teale A. Highly unsaturated fatty acid synthesis in vertebrates: new insights with the cloning and characterization of a delta6 desaturase of Atlantic salmon. Lipids. 2005;40:13-24 pubmed..Furthermore, expression of both delta6 and delta5 fatty acyl desaturase genes in intestine, liver, red muscle, and adipose tissue was higher in salmon fed a diet containing vegetable oil than in fish fed a diet containing fish oil...
- Agaba M, Tocher D, Zheng X, Dickson C, Dick J, Teale A. Cloning and functional characterisation of polyunsaturated fatty acid elongases of marine and freshwater teleost fish. Comp Biochem Physiol B Biochem Mol Biol. 2005;142:342-52 pubmed..The fish elongases generally showed greater activity or similar activities with n-3 than with n-6 homologues, with the exception of the cod enzyme which was more active towards n-6 fatty acids. ..
- Trinkle Mulcahy L, Andersen J, Lam Y, Moorhead G, Mann M, Lamond A. Repo-Man recruits PP1 gamma to chromatin and is essential for cell viability. J Cell Biol. 2006;172:679-92 pubmed..The data indicate that Repo-Man forms an essential complex with PP1gamma and is required for the recruitment of PP1 to chromatin. ..
- Gibellini F, Hunter W, Smith T. Biochemical characterization of the initial steps of the Kennedy pathway in Trypanosoma brucei: the ethanolamine and choline kinases. Biochem J. 2008;415:135-44 pubmed publisher..Both enzymes recognized analogues with substituents on C-2, but substitutions on C-1 and elongations of the carbon chain were not well tolerated...
- Perona Wright G, Jenkins S, O Connor R, Zienkiewicz D, McSorley H, Maizels R, et al. A pivotal role for CD40-mediated IL-6 production by dendritic cells during IL-17 induction in vivo. J Immunol. 2009;182:2808-15 pubmed publisher..Our data delineate sequential requirements for DC expression of CD40 and production of IL-6 during Th17 polarization in vitro and in vivo, and reveal distinct costimulatory requirements for Th1 vs Th17 generation. ..
- Smith H, Peggie M, Campbell D, Vandermoere F, Carrick E, Cohen P. Identification of the phosphorylation sites on the E3 ubiquitin ligase Pellino that are critical for activation by IRAK1 and IRAK4. Proc Natl Acad Sci U S A. 2009;106:4584-90 pubmed publisher..These observations imply that dephosphorylation of multiple sites is required to inactivate Pellino 1, which could be a device for prolonging Pellino's E3 ubiquitin ligase activity in vivo. ..
- Lebaron S, Segerstolpe A, French S, Dudnakova T, de Lima Alves F, Granneman S, et al. Rrp5 binding at multiple sites coordinates pre-rRNA processing and assembly. Mol Cell. 2013;52:707-19 pubmed publisher..A key role in coordinating preribosomal assembly and processing was confirmed by chromatin spreads. Following depletion of Rrp5, cotranscriptional cleavage was lost and preribosome compaction greatly reduced. ..
- Loenen W, Daniel A, Braymer H, Murray N. Organization and sequence of the hsd genes of Escherichia coli K-12. J Mol Biol. 1987;198:159-70 pubmed..The R polypeptide shows homology with a variety of ATPases, and pronounced regions of alpha-helical structure are predicted, one of which is amphipathic. ..
- O Dell K, Armstrong J, Yang M, Kaiser K. Functional dissection of the Drosophila mushroom bodies by selective feminization of genetically defined subcompartments. Neuron. 1995;15:55-61 pubmed..Expression of transformer in other mushroom body domains, and in control lines, has no such effect. Our data support the view that genetically defined subsets of mushroom body intrinsic neurons perform different functional roles...
- Glover D, Leibowitz M, McLean D, Parry H. Mutations in aurora prevent centrosome separation leading to the formation of monopolar spindles. Cell. 1995;81:95-105 pubmed..The size of the single centrosomal body in these circular figures suggests that loss of function of the serine-threonine protein kinase encoded by aur leads to a failure of the centrosomes to separate and form a bipolar spindle. ..
- Roff M, Thompson J, Rodriguez M, Jacque J, Baleux F, Arenzana Seisdedos F, et al. Role of IkappaBalpha ubiquitination in signal-induced activation of NFkappaB in vivo. J Biol Chem. 1996;271:7844-50 pubmed..Thus signal-induced activation of NF-kappaB involves phosphorylation-dependent ubiquitination of IkappaBalpha, which targets the protein for rapid degradation by the proteasome and releases NF-kappaB for translocation to the nucleus. ..
- Nicoll G, Ni J, Liu D, Klenerman P, Munday J, Dubock S, et al. Identification and characterization of a novel siglec, siglec-7, expressed by human natural killer cells and monocytes. J Biol Chem. 1999;274:34089-95 pubmed..Immunoprecipitation experiments indicated that siglec-7 is expressed as a monomer of approximately 65 kDa. ..
- Campbell L, Hardwick K. Analysis of Bub3 spindle checkpoint function in Xenopus egg extracts. J Cell Sci. 2003;116:617-28 pubmed..We discuss the mechanism by which our alpha-XBub3 antibodies interfere with the checkpoint and possible roles for XBub3 in the spindle checkpoint pathway. ..
- Westwood P, Martin I, Fantes P. Fission yeast Cdc37 is required for multiple cell cycle functions. Mol Genet Genomics. 2004;271:82-90 pubmed..The cells arrest heterogeneously, with a significant proportion showing mitotic defects; paradoxically, a proportion of the cells show a short-cell phenotype consistent with an advanced cell cycle. ..
- McHugh B, Krause S, Yu B, Deans A, Heasman S, McLaughlin P, et al. Invadolysin: a novel, conserved metalloprotease links mitotic structural rearrangements with cell migration. J Cell Biol. 2004;167:673-86 pubmed..Thus, invadolysin identifies a new family of conserved metalloproteases whose activity appears to be essential for the coordination of mitotic progression, but which also plays an unexpected role in cell migration. ..
- Jaleel M, McBride A, Lizcano J, Deak M, Toth R, Morrice N, et al. Identification of the sucrose non-fermenting related kinase SNRK, as a novel LKB1 substrate. FEBS Lett. 2005;579:1417-23 pubmed..These results provide evidence that SNRK could mediate some of the physiological effects of LKB1. ..
- Friedrich J, Panov K, Cabart P, Russell J, Zomerdijk J. TBP-TAF complex SL1 directs RNA polymerase I pre-initiation complex formation and stabilizes upstream binding factor at the rDNA promoter. J Biol Chem. 2005;280:29551-8 pubmed
- Longhurst D, Watanabe M, Rothstein J, Jackson M. Interaction of PDZRhoGEF with microtubule-associated protein 1 light chains: link between microtubules, actin cytoskeleton, and neuronal polarity. J Biol Chem. 2006;281:12030-40 pubmed..This coordinated action of the actin and microtubular cytoskeletons is essential for the development and maintenance of neuronal polarity. ..
- Szoor B, Wilson J, McElhinney H, Tabernero L, Matthews K. Protein tyrosine phosphatase TbPTP1: A molecular switch controlling life cycle differentiation in trypanosomes. J Cell Biol. 2006;175:293-303 pubmed..It may be possible to use PTP1B inhibitors to block trypanosomatid transmission. ..
- Sibthorpe D, Sturlaugsdóttir R, Kristjansson B, Thorarensen H, Skulason S, Johnston I. Characterisation and expression of the paired box protein 7 (Pax7) gene in polymorphic Arctic charr (Salvelinus alpinus). Comp Biochem Physiol B Biochem Mol Biol. 2006;145:371-83 pubmed..The potential role of multiple alternative splicing of the Pax7 gene for the generation of different Arctic charr morphs is briefly discussed. ..
- Lavery D, McEwan I. The human androgen receptor AF1 transactivation domain: interactions with transcription factor IIF and molten-globule-like structural characteristics. Biochem Soc Trans. 2006;34:1054-7 pubmed..We hypothesize that this partially folded intermediate state is important for, and enables the AF1 domain to make, multiple protein-protein interactions. The structural aspects of AR-AF1 and interactions with TFIIF are discussed. ..
- Lukashev A, Ivanova O, Eremeeva T, Iggo R. Evidence of frequent recombination among human adenoviruses. J Gen Virol. 2008;89:380-8 pubmed publisher..Comparison of the right end (nt 18838-33452) of this isolate with that of the ATCC Ad6 strain showed clear evidence of multiple recombination events...
- Dardente H, Birnie M, Lincoln G, Hazlerigg D. RFamide-related peptide and its cognate receptor in the sheep: cDNA cloning, mRNA distribution in the hypothalamus and the effect of photoperiod. J Neuroendocrinol. 2008;20:1252-9 pubmed publisher..Taken together, these data strengthen the emerging view that interplay between ependymal cells and the pars tuberalis might be important for the seasonal timing system...
- Thein M, Winter A, Stepek G, McCormack G, Stapleton G, Johnstone I, et al. Combined extracellular matrix cross-linking activity of the peroxidase MLT-7 and the dual oxidase BLI-3 is critical for post-embryonic viability in Caenorhabditis elegans. J Biol Chem. 2009;284:17549-63 pubmed publisher..This points toward a functionally cooperative relationship for these two hypodermally expressed proteins that is essential for collagen cross-linking and proper extracellular matrix formation. ..
- Laursen L, Chan C, Ffrench Constant C. Translation of myelin basic protein mRNA in oligodendrocytes is regulated by integrin activation and hnRNP-K. J Cell Biol. 2011;192:797-811 pubmed publisher..Furthermore, knockdown of hnRNP-K inhibits MBP protein synthesis during myelination. Together, these results identify a novel pathway by which axoglial adhesion molecules coordinate MBP synthesis with myelin sheath formation. ..
- Klejnot M, Kozielski F. Structural insights into human Kif7, a kinesin involved in Hedgehog signalling. Acta Crystallogr D Biol Crystallogr. 2012;68:154-9 pubmed publisher..These data are a first step towards structural characterization of a kinesin-4 family member and of this interesting molecular motor of medical significance. ..
- Alessi D, MacDougall L, Sola M, Ikebe M, Cohen P. The control of protein phosphatase-1 by targetting subunits. The major myosin phosphatase in avian smooth muscle is a novel form of protein phosphatase-1. Eur J Biochem. 1992;210:1023-35 pubmed
- Warbrick E, Lane D, Glover D, Cox L. A small peptide inhibitor of DNA replication defines the site of interaction between the cyclin-dependent kinase inhibitor p21WAF1 and proliferating cell nuclear antigen. Curr Biol. 1995;5:275-82 pubmed..abstract truncated at 250 words) ..
- Curran J, Argyle D, Cox P, Onions D, Nicolson L. Nucleotide sequence of the equine interferon gamma cDNA. DNA Seq. 1994;4:405-7 pubmed..The predicted equine IFN gamma amino acid sequence is 67% identical to that of the human equivalent and 78% to the bovine equivalent. ..
- Thompson J, Doxakis E, Pinon L, Strachan P, Buj Bello A, Wyatt S, et al. GFRalpha-4, a new GDNF family receptor. Mol Cell Neurosci. 1998;11:117-26 pubmed..Our findings extend the family of GFRalpha proteins and provide information about the tissues in which GFRalpha-4 may function during development. ..
- Floyd H, Ni J, Cornish A, Zeng Z, Liu D, Carter K, et al. Siglec-8. A novel eosinophil-specific member of the immunoglobulin superfamily. J Biol Chem. 2000;275:861-6 pubmed..Using specific monoclonal antibodies, siglec-8 could be detected only on eosinophils and hence appears to be the first example of an eosinophil-specific transmembrane receptor. ..
- Hastie C, Carnegie G, Morrice N, Cohen P. A novel 50 kDa protein forms complexes with protein phosphatase 4 and is located at centrosomal microtubule organizing centres. Biochem J. 2000;347 Pt 3:845-55 pubmed..Native 450 kDa and 600 kDa PPP4 complexes are inactive, but can be activated by basic proteins, suggesting that PPP4R2 may also regulate the activity of PPP4c at centrosomal microtubule organising centres. ..
- Chamberlain L, Burgoyne R. Cysteine-string protein: the chaperone at the synapse. J Neurochem. 2000;74:1781-9 pubmed..Targets for Csp include the vesicle protein VAMP/synaptobrevin and the plasma membrane protein syntaxin 1, the significance of which is discussed in possible models to account for current knowledge of Csp function. ..
- Mackenzie S, Baillie G, McPhee I, Bolger G, Houslay M. ERK2 mitogen-activated protein kinase binding, phosphorylation, and regulation of the PDE4D cAMP-specific phosphodiesterases. The involvement of COOH-terminal docking sites and NH2-terminal UCR regions. J Biol Chem. 2000;275:16609-17 pubmed..The PDE4D gene thus encodes a series of isoenzymes that are either inhibited or activated by ERK2 phosphorylation and thereby offers the potential for ERK2 activation either to increase or decrease cAMP levels in cellular compartments. ..
- Cheung P, Trinkle Mulcahy L, Cohen P, Lucocq J. Characterization of a novel phosphatidylinositol 3-phosphate-binding protein containing two FYVE fingers in tandem that is targeted to the Golgi. Biochem J. 2001;355:113-21 pubmed..Cell localization studies using a green fluorescent protein fusion show that TAFF1 is localized to the Golgi, and that the Golgi targeting sequence is located within the N-terminal 187 residues and not in either FYVE domain. ..
- Roszak A, Robinson D, Krell T, Hunter I, Fredrickson M, Abell C, et al. The structure and mechanism of the type II dehydroquinase from Streptomyces coelicolor. Structure. 2002;10:493-503 pubmed..The active site of the structure of DHQase in complex with the transition state analog also includes molecules of tartrate and glycerol, which provide a basis for further inhibitor design...
- Bridgen A, Dalrymple D, Elliott R. Dugbe nairovirus S segment: correction of published sequence and comparison of five isolates. Virology. 2002;294:364-71 pubmed..Phylogenetic trees were constructed for these nairovirus S segment sequences. ..
- Winter A, Myllyharju J, Page A. A hypodermally expressed prolyl 4-hydroxylase from the filarial nematode Brugia malayi is soluble and active in the absence of protein disulfide isomerase. J Biol Chem. 2003;278:2554-62 pubmed..elegans. Although active by itself, the B. malayi PHY-1 was not able to replace enzyme function in a C. elegans P4H mutant...
- Jowsey I, Thomson R, Orton T, Elcombe C, Hayes J. Biochemical and genetic characterization of a murine class Kappa glutathione S-transferase. Biochem J. 2003;373:559-69 pubmed..Furthermore, the association of these enzymes with mitochondrial fractions is consistent with them performing a specific conserved biological role within this organelle. ..
- Hickson G, Matheson J, Riggs B, Maier V, Fielding A, Prekeris R, et al. Arfophilins are dual Arf/Rab 11 binding proteins that regulate recycling endosome distribution and are related to Drosophila nuclear fallout. Mol Biol Cell. 2003;14:2908-20 pubmed
- Wimmer B, Craig B, Pilkington J, Pemberton J. Non-invasive assessment of parasitic nematode species diversity in wild Soay sheep using molecular markers. Int J Parasitol. 2004;34:625-31 pubmed..The method was also tested for its applicability to high through-put screening of faecal samples. ..
- Feng X, Colloms S. In vitro transposition of ISY100, a bacterial insertion sequence belonging to the Tc1/mariner family. Mol Microbiol. 2007;65:1432-43 pubmed..Our results demonstrate that there are no fundamental differences between the transposition mechanisms of IS630 family elements in bacteria and Tc1/mariner elements in higher eukaryotes. ..
- Macqueen D, Johnston I. Evolution of follistatin in teleosts revealed through phylogenetic, genomic and expression analyses. Dev Genes Evol. 2008;218:1-14 pubmed..Later, fst1 was expressed in myogenic progenitors of the pectoral fin buds and also within the pax7(+) cell layer external to the myotome. ..
- D az Rosales P, Bird S, Wang T, Fujiki K, Davidson W, Zou J, et al. Rainbow trout interleukin-2: cloning, expression and bioactivity analysis. Fish Shellfish Immunol. 2009;27:414-22 pubmed publisher..The potential signalling pathways involved and possible use as an adjuvant for fish vaccines are discussed...
- Alberdi M, Watson E, McAllister G, Harris J, Paxton E, Thomson J, et al. Expression by Lawsonia intracellularis of type III secretion system components during infection. Vet Microbiol. 2009;139:298-303 pubmed publisher..By analogy to other bacteria, this T3SS may be crucial for intracellular development and is likely to play a significant role in the virulence of this unusual pathogen...
- Richardson E, Limaye B, Inamdar H, Datta A, Manjari K, Pullinger G, et al. Genome sequences of Salmonella enterica serovar typhimurium, Choleraesuis, Dublin, and Gallinarum strains of well- defined virulence in food-producing animals. J Bacteriol. 2011;193:3162-3 pubmed publisher..This provides valuable measures of intraserovar diversity and opportunities to formally link genotypes to phenotypes in target animals. ..
- Fiskerstrand C, Roy D, Green I, Sargan D. Cloning, expression and characterization of ovine interleukins 1 alpha and beta. Cytokine. 1992;4:418-28 pubmed..The presence of a Factor Xa cleavage site in the IL-1 alpha proprotein suggests that Factor Xa may be involved in the processing of ovine IL-1 alpha to its mature form. ..
- Moisan M, Edwards C, Seckl J. Differential promoter usage by the rat 11 beta-hydroxysteroid dehydrogenase gene. Mol Endocrinol. 1992;6:1082-7 pubmed
- Dent P, Campbell D, Hubbard M, Cohen P. Multisite phosphorylation of the glycogen-binding subunit of protein phosphatase-1G by cyclic AMP-dependent protein kinase and glycogen synthase kinase-3. FEBS Lett. 1989;248:67-72 pubmed
- Jacobs H, Elliott D, Math V, Farquharson A. Nucleotide sequence and gene organization of sea urchin mitochondrial DNA. J Mol Biol. 1988;202:185-217 pubmed..The separation of the ribosomal RNA genes from each other and from the putative control region imposes special demands on the transcription of the genome...
- Sinclair M, Gilchrist J, Aitken R. Bovine IgG repertoire is dominated by a single diversified VH gene family. J Immunol. 1997;159:3883-9 pubmed..Comparison of germline VH sequences with those gathered from adult bovine splenic cDNA demonstrates a pattern of nucleotide substitution that is consistent with diversification through somatic hypermutation. ..
- McClive P, Pall G, Newton K, Lee M, Mullins J, Forrester L. Gene trap integrations expressed in the developing heart: insertion site affects splicing of the PT1-ATG vector. Dev Dyn. 1998;212:267-76 pubmed..One of these exon integrations results in a lethal neonatal phenotype. ..
- Millar C, Powell K, Hickson G, Bader M, Gould G. Evidence for a role for ADP-ribosylation factor 6 in insulin-stimulated glucose transporter-4 (GLUT4) trafficking in 3T3-L1 adipocytes. J Biol Chem. 1999;274:17619-25 pubmed..These data suggest an important role for ARF6 in regulating cell surface levels of GLUT4 in adipocytes, and argue for a role for both ARF5 and ARF6 in the regulation of membrane trafficking to the plasma membrane. ..