Genomes and Genes
Experts and Doctors on molecular sequence data in North Carolina, United States
Locale: North Carolina, United States
Topic: molecular sequence data
Publications159 found, 100 shown here
- Lai W, Kennington E, Blackshear P. Interactions of CCCH zinc finger proteins with mRNA: non-binding tristetraprolin mutants exert an inhibitory effect on degradation of AU-rich element-containing mRNAs. J Biol Chem. 2002;277:9606-13 pubmed..These data suggest that TTP may act, at least in part, by physically interacting with an enzyme activity or protein complex and functionally stimulating its ability to deadenylate class II ARE-containing mRNAs. ..
- Ponamarev M, Longley M, Nguyen D, Kunkel T, Copeland W. Active site mutation in DNA polymerase gamma associated with progressive external ophthalmoplegia causes error-prone DNA synthesis. J Biol Chem. 2002;277:15225-8 pubmed..The error-prone DNA synthesis observed for the Y955C pol gamma is consistent with the accumulation of mtDNA mutations in patients with PEO. ..
- Carrick D, Lai W, Blackshear P. The tandem CCCH zinc finger protein tristetraprolin and its relevance to cytokine mRNA turnover and arthritis. Arthritis Res Ther. 2004;6:248-64 pubmed..The TTP pathway of TNF-alpha and GM-CSF mRNA degradation is a possible novel target for anti-TNF-alpha therapies for rheumatoid arthritis, and also for other conditions proven to respond to anti-TNF-alpha therapy. ..
- Graziewicz M, Longley M, Copeland W. DNA polymerase gamma in mitochondrial DNA replication and repair. Chem Rev. 2006;106:383-405 pubmed
- Shi H, Teng C. Characterization of a mitogen-response unit in the mouse lactoferrin gene promoter. J Biol Chem. 1994;269:12973-80 pubmed..The mobility shift assay suggested that AP1 and CREB or related proteins participated in complex formation with the mLF-CRE, whereas different proteins bound to the EGF/TGF-alpha-response element. ..
- Jetten A, Bernacki S, Floyd E, Saunders N, Pieniazek J, Lotan R. Expression of a preprorelaxin-like gene during squamous differentiation of rabbit tracheobronchial epithelial cells and its suppression by retinoic acid. Cell Growth Differ. 1992;3:549-56 pubmed..The concentration at which retinoic acid caused a 50% inhibition of SQ10 mRNA levels was approximately 5 nM.(ABSTRACT TRUNCATED AT 250 WORDS) ..
- Novak P, Dev I. Degradation of a signal peptide by protease IV and oligopeptidase A. J Bacteriol. 1988;170:5067-75 pubmed
- Wood E, Berger H, Sherman P, Lapetina E. Hepatocytes and macrophages express an identical cytokine inducible nitric oxide synthase gene. Biochem Biophys Res Commun. 1993;191:767-74 pubmed..These differences are most likely the result of species variability which suggests that hepatocytes and macrophages express the same NOS gene upon induction. ..
- Gu L, Huang S, Sander M. Single amino acid changes alter the repair specificity of Drosophila Rrp1. Isolation of mutants deficient in repair of oxidative DNA damage. J Biol Chem. 1994;269:32685-92 pubmed..Glu-461 appears to be essential for the nuclease function for Rrp1. Lys-463 and, to a lesser extent, Thr-462 influence the substrate specificity of the Rrp1 nuclease. ..
- Burnett V, Lawton M, Philpot R. Cloning and sequencing of flavin-containing monooxygenases FMO3 and FMO4 from rabbit and characterization of FMO3. J Biol Chem. 1994;269:14314-22 pubmed..Rabbit genomic DNA was probed under conditions of low stringency with a mixture of 5' cDNA fragments encoding all five FMO forms and produced results consistent with the possibility of one additional FMO. ..
- Itagaki K, Carver G, Philpot R. Expression and characterization of a modified flavin-containing monooxygenase 4 from humans. J Biol Chem. 1996;271:20102-7 pubmed..As with FMO4, the difference in expression levels between FMO3 and elongated FMO3 (FMO3*) appears to be related to translation rather than transcription. The functional characteristics of FMO3 and FMO3* are not significantly different. ..
- Minnick D, Astatke M, Joyce C, Kunkel T. A thumb subdomain mutant of the large fragment of Escherichia coli DNA polymerase I with reduced DNA binding affinity, processivity, and frameshift fidelity. J Biol Chem. 1996;271:24954-61 pubmed..The results are discussed in light of remarkably similar observations with T7 DNA polymerase in the presence or absence of thioredoxin, an accessory subunit that affects these same properties. ..
- Staudinger J, Lu J, Olson E. Specific interaction of the PDZ domain protein PICK1 with the COOH terminus of protein kinase C-alpha. J Biol Chem. 1997;272:32019-24 pubmed..Association of PKCalpha with PICK1 provides a potential mechanism for the selective targeting of PKCalpha to unique subcellular sites. ..
- Kliewer S, Moore J, Wade L, Staudinger J, Watson M, Jones S, et al. An orphan nuclear receptor activated by pregnanes defines a novel steroid signaling pathway. Cell. 1998;92:73-82 pubmed..Our results provide evidence for the existence of a novel steroid hormone signaling pathway with potential implications in the regulation of steroid hormone and sterol homeostasis. ..
- Abbott B, Schmid J, Brown J, Wood C, White R, Buckalew A, et al. RT-PCR quantification of AHR, ARNT, GR, and CYP1A1 mRNA in craniofacial tissues of embryonic mice exposed to 2,3,7,8-tetrachlorodibenzo-p-dioxin and hydrocortisone. Toxicol Sci. 1999;47:76-85 pubmed
- Zhang F, Nakanishi G, Kurebayashi S, Yoshino K, Perantoni A, Kim Y, et al. Characterization of Glis2, a novel gene encoding a Gli-related, Krüppel-like transcription factor with transactivation and repressor functions. Roles in kidney development and neurogenesis. J Biol Chem. 2002;277:10139-49 pubmed..Our results suggest that Glis2 behaves as a bifunctional transcriptional regulator. Both the activation and repressor functions may play an important role in the regulation of gene expression during embryonic development. ..
- Bebenek K, Garcia Diaz M, Patishall S, Kunkel T. Biochemical properties of Saccharomyces cerevisiae DNA polymerase IV. J Biol Chem. 2005;280:20051-8 pubmed..These properties are consistent with a possible role for pol IV in base excision repair and with its known role in non-homologous end joining of double strand breaks, perhaps including those with damaged ends. ..
- Nakamura N, Shibata H, O Brien D, Mori C, Eddy E. Spermatogenic cell-specific type 1 hexokinase is the predominant hexokinase in sperm. Mol Reprod Dev. 2008;75:632-40 pubmed..These results strongly suggest that HK1, HK2, HK3, and GCK are unlikely to have a role in glycolysis in sperm and that HK1S encoded by Hk1_v2 and Hk1_v3 serves this role. ..
- Cavanaugh N, Beard W, Wilson S. DNA polymerase beta ribonucleotide discrimination: insertion, misinsertion, extension, and coding. J Biol Chem. 2010;285:24457-65 pubmed publisher..These results suggest that the sugar pucker at the primer terminus plays a crucial role in DNA synthesis; a 3'-endo sugar pucker facilitates nucleotide insertion, whereas a 2'-endo conformation inhibits insertion. ..
- Lehmann J, McKee D, Watson M, Willson T, Moore J, Kliewer S. The human orphan nuclear receptor PXR is activated by compounds that regulate CYP3A4 gene expression and cause drug interactions. J Clin Invest. 1998;102:1016-23 pubmed..These findings provide a molecular explanation for the ability of disparate chemicals to induce CYP3A4 levels and, furthermore, provide a basis for developing in vitro assays to aid in predicting whether drugs will interact in humans. ..
- Gardlik S, Gasser R, Philpot R, Serabjit Singh C. The major alpha-class glutathione S-transferases of rabbit lung and liver. Primary sequences, expression, and regulation. J Biol Chem. 1991;266:19681-7 pubmed..This tissue- and species-dependent expression of the predominant class of cytosolic GST implies unique functions for each isozyme and may contribute to the differential susceptibility of tissues and animals to toxicants. ..
- Fukasawa K, Li W, Yagi K, Luo C, Li S. Molecular evolution of mammalian lactate dehydrogenase-A genes and pseudogenes: association of a mouse processed pseudogene with a B1 repetitive sequence. Mol Biol Evol. 1986;3:330-42 pubmed..20 X 10(-9), which is considerably lower than the average rate of 0.88 X 10(-9) for 35 mammalian genes. Thus, the mammalian LDH-A gene appears to be highly conserved in evolution. ..
- Mannen H, Tsoi S, Krushkal J, Li W, Li S. The cDNA cloning and molecular evolution of reptile and pigeon lactate dehydrogenase isozymes. Mol Biol Evol. 1997;14:1081-7 pubmed publisher..The pigeon testicular LDH-C isozyme was evidently duplicated from LDH-B (heart), so it is not orthologous to the mammalian testicular LDH-C isozymes...
- Miller S, Scappini E, O BRYAN J. Ubiquitin-interacting motifs inhibit aggregation of polyQ-expanded huntingtin. J Biol Chem. 2007;282:10096-103 pubmed..Furthermore, we found that expression of single, isolated UIMs inhibited aggregation of mutant Htt. These data suggest that isolated UIMs might serve as potential inhibitors of polyQ-aggregation in vivo. ..
- Qiu C, Kershner A, Wang Y, Holley C, Wilinski D, Keles S, et al. Divergence of Pumilio/fem-3 mRNA binding factor (PUF) protein specificity through variations in an RNA-binding pocket. J Biol Chem. 2012;287:6949-57 pubmed publisher..Loss of the pocket requires only substitution of one serine, as appears to have occurred during the evolution of certain fungal species. ..
- Wong G, Kawajiri K, Negishi M. Gene family of male-specific testosterone 16 alpha-hydroxylase (C-P-450(16) alpha) in mouse liver: cDNA sequences, neonatal imprinting, and reversible regulation by androgen. Biochemistry. 1987;26:8683-90 pubmed..The putative poly(A) addition signal AATAAA is present for all three clones, but there are polymorphisms in the start sites of polyadenylation.(ABSTRACT TRUNCATED AT 250 WORDS) ..
- Willard D, Bodnar W, Harris C, Kiefer L, Nichols J, Blanchard S, et al. Agouti structure and function: characterization of a potent alpha-melanocyte stimulating hormone receptor antagonist. Biochemistry. 1995;34:12341-6 pubmed..The isolated highly cysteine-rich C-terminal domain retains alpha-MSH antagonism equipotent with mature agouti. This bioactive domain contains all 10 cysteines which exhibit sequence homology when aligned with several conotoxins. ..
- Moore J, McKee D, Slentz Kesler K, Moore L, Jones S, Horne E, et al. Cloning and characterization of human estrogen receptor beta isoforms. Biochem Biophys Res Commun. 1998;247:75-8 pubmed
- Bidwell M, Eitzman B, Walmer D, McLachlan J, Gray K. Analysis of messenger ribonucleic acid and protein for the ligands and receptors of the platelet-derived growth factor signaling pathway in the placenta, extraembryonic membranes, and uterus during the latter half of murine gestation. Endocrinology. 1995;136:5189-201 pubmed
- Peterson S, Trabalzini L, Brtva T, Fischer T, Altschuler D, Martelli P, et al. Identification of a novel RalGDS-related protein as a candidate effector for Ras and Rap1. J Biol Chem. 1996;271:29903-8 pubmed..We conclude that RGL2 may be an effector for Ras and/or Rap proteins. ..
- Blackshear P, Phillips R, Ghosh S, Ramos S, Ramos S, Richfield E, et al. Zfp36l3, a rodent X chromosome gene encoding a placenta-specific member of the Tristetraprolin family of CCCH tandem zinc finger proteins. Biol Reprod. 2005;73:297-307 pubmed..Its presumed role in placental physiology may be unique to rodents or murine rodents, but this role may be subsumed by other family members in nonrodents. ..
- Mori C, Welch J, Sakai Y, Eddy E. In situ localization of spermatogenic cell-specific glyceraldehyde 3-phosphate dehydrogenase (Gapd-s) messenger ribonucleic acid in mice. Biol Reprod. 1992;46:859-68 pubmed
- Nugent M, Huang S, Sander M. Characterization of the apurinic endonuclease activity of Drosophila Rrp1. Biochemistry. 1993;32:11445-52 pubmed..Therefore, Rrp1 cleaves the phosphodiester backbone at one position 5' to the apurinic site and leaves a 3'-hydroxyl terminus. Rrp1 is a class II apurinic endonuclease and is likely to be important in DNA repair in Drosophila. ..
- King L, Ma J, Srettabunjong S, Graves J, Bradbury J, Li L, et al. Cloning of CYP2J2 gene and identification of functional polymorphisms. Mol Pharmacol. 2002;61:840-52 pubmed..Insofar as CYP2J2 products have effects in the cardiovascular system, we speculate that these variants may be functionally relevant. ..
- Shcherbakova P, Pavlov Y, Chilkova O, Rogozin I, Johansson E, Kunkel T. Unique error signature of the four-subunit yeast DNA polymerase epsilon. J Biol Chem. 2003;278:43770-80 pubmed..We discuss the implications of these findings for the role of Pol epsilon polymerase activity in DNA replication. ..
- Ichikawa T, Itakura T, Negishi M. Functional characterization of two cytochrome P-450s within the mouse, male-specific steroid 16 alpha-hydroxylase gene family: expression in mammalian cells and chimeric proteins. Biochemistry. 1989;28:4779-84 pubmed
- Wong G, Itakura T, Kawajiri K, Skow L, Negishi M. Gene family of male-specific testosterone 16 alpha-hydroxylase (C-P-450(16 alpha)) in mice. Organization, differential regulation, and chromosome localization. J Biol Chem. 1989;264:2920-7 pubmed..The recombination frequency in BXD recombinant inbred mice suggests that the C-P-450(16 alpha) gene family is approximately 16M from the 55-kDa locus. ..
- Dittman W, Nelson S, Greer P, Horton E, Palomba M, McCachren S. Characterization of thrombomodulin expression in response to retinoic acid and identification of a retinoic acid response element in the human thrombomodulin gene. J Biol Chem. 1994;269:16925-32 pubmed..Deletion of, and mutations introduced into, the potential retinoic acid response element confirm the functional response in transient transfections. ..
- Ikeda Y, Lala D, Luo X, Kim E, Moisan M, Parker K. Characterization of the mouse FTZ-F1 gene, which encodes a key regulator of steroid hydroxylase gene expression. Mol Endocrinol. 1993;7:852-60 pubmed..ELP transcripts were not detected from embryonic day 8 to adult, consistent with its previous isolation from embryonal carcinoma cells and its postulated role in early embryonic development.(ABSTRACT TRUNCATED AT 250 WORDS) ..
- G rlach J, Volrath S, Knauf Beiter G, Hengy G, Beckhove U, Kogel K, et al. Benzothiadiazole, a novel class of inducers of systemic acquired resistance, activates gene expression and disease resistance in wheat. Plant Cell. 1996;8:629-43 pubmed publisher..BTH is being developed commercially as a novel type of plant protection compound that works by inducing the plant's inherent disease resistance mechanisms...
- Garvey E, Tuttle J, Covington K, Merrill B, Wood E, Baylis S, et al. Purification and characterization of the constitutive nitric oxide synthase from human placenta. Arch Biochem Biophys. 1994;311:235-41 pubmed..Together, these data indicate that the placental NOS is the constitutive NOS isozyme from endothelial tissue. ..
- Uknes S, Mauch Mani B, Moyer M, Potter S, Williams S, Dincher S, et al. Acquired resistance in Arabidopsis. Plant Cell. 1992;4:645-56 pubmed..Arabidopsis should serve as a genetic model for studies of this type of immune response in plants. ..
- Yokomori N, Kobayashi R, Moore R, Sueyoshi T, Negishi M. A DNA methylation site in the male-specific P450 (Cyp 2d-9) promoter and binding of the heteromeric transcription factor GABP. Mol Cell Biol. 1995;15:5355-62 pubmed..We also conclude that GABP is a methylation-sensitive transcription factor and is a potential transcription activator of the male-specific Cyp 2d-9 promoter. ..
- Rosenberg M, Aversa C, Wallace R, Propst F. Expression of the v-Mos oncogene in male meiotic germ cells of transgenic mice results in metaphase arrest. Cell Growth Differ. 1995;6:325-36 pubmed..Thus, overexpression of pp39v-mos during male germ cell meiosis resulted in an alteration of various cell cycle related kinases and cytostatic factor-like arrest at MI. ..
- Liu Y, Yang N, Teng C. COUP-TF acts as a competitive repressor for estrogen receptor-mediated activation of the mouse lactoferrin gene. Mol Cell Biol. 1993;13:1836-46 pubmed..Our results indicate that two members of the steroid-thyroid receptor superfamily work in concert to modulate lactoferrin gene expression. ..
- Tran H, Gordenin D, Resnick M. The prevention of repeat-associated deletions in Saccharomyces cerevisiae by mismatch repair depends on size and origin of deletions. Genetics. 1996;143:1579-87 pubmed..In contrast to the pol3-t, the enhancement of 1-bp deletions in a rad52 mutant is not altered by a pms1 mutation. Thus, mismatch repair appears to be specific to errors of DNA synthesis generated during semiconservative replication. ..
- Ma J, Qu W, Scarborough P, Tomer K, Moomaw C, Maronpot R, et al. Molecular cloning, enzymatic characterization, developmental expression, and cellular localization of a mouse cytochrome P450 highly expressed in kidney. J Biol Chem. 1999;274:17777-88 pubmed..In light of the well documented effects of epoxyeicosatrienoic acids in modulating renal tubular transport processes, we postulate that CYP2J5 products play important functional roles in the kidney. ..
- Andrews K, Potdar P, Nettesheim P, Ostrowski L. KPL1, which encodes a novel PH domain-containing protein, is induced during ciliated cell differentiation of rat tracheal epithelial cells. Exp Lung Res. 2000;26:257-71 pubmed..As a new member of the family of PH domain-containing proteins, KPL1 may have a unique role in ciliated cell differentiation or function. ..
- Wang X, Tanaka Hall T. Structural basis for recognition of AU-rich element RNA by the HuD protein. Nat Struct Biol. 2001;8:141-5 pubmed..Different conformations of nucleic acid can be bound by RRM domains by using different combinations of base recognition pockets and multiple RRM domains. ..
- Qin L, Block M, Liu Y, Bienstock R, Pei Z, Zhang W, et al. Microglial NADPH oxidase is a novel target for femtomolar neuroprotection against oxidative stress. FASEB J. 2005;19:550-7 pubmed
- Mutoh S, Osabe M, Inoue K, Moore R, PEDERSEN L, Perera L, et al. Dephosphorylation of threonine 38 is required for nuclear translocation and activation of human xenobiotic receptor CAR (NR1I3). J Biol Chem. 2009;284:34785-92 pubmed publisher..These results define CAR as a cell signal-regulated constitutive active nuclear receptor. These results also provide phosphorylation/dephosphorylation of the threonine as the primary drug target for CAR activation. ..
- Hutti J, Porter M, Cheely A, Cantley L, Wang X, Kireev D, et al. Development of a high-throughput assay for identifying inhibitors of TBK1 and IKK?. PLoS ONE. 2012;7:e41494 pubmed publisher..Together, these data describe a new high-throughput screening assay which will facilitate the discovery of small molecule TBK1/IKK? inhibitors possessing therapeutic potential for both inflammatory diseases and cancer. ..
- Flanagan T, Taylor L, Poulter L, Viveros O, Diliberto E. A novel 1745-dalton pyroglutamyl peptide derived from chromogranin B is in the bovine adrenomedullary chromaffin vesicle. Cell Mol Neurobiol. 1990;10:507-23 pubmed..3. A computer search through the Swiss-Prot protein sequence database revealed a 93% identity of BAM-1745 and a fragment of human chromogranin B (Gln580-Tyr593). ..
- Dev I, Ray P, Novak P. Minimum substrate sequence for signal peptidase I of Escherichia coli. J Biol Chem. 1990;265:20069-72 pubmed..The nonapeptide is the most efficient substrate for the enzyme in vitro so far reported. It is speculated that better peptide substrates are the ones which are able to adopt folded structures. ..
- Porter D, Merrill B, Short S. Identification of the active site nucleophile in nucleoside 2-deoxyribosyltransferase as glutamic acid 98. J Biol Chem. 1995;270:15551-6 pubmed..Collectively, chemical modification and mutagenesis studies have identified Glu-98 as the active site nucleophile of nucleoside 2-deoxyribosyltransferase...
- Laethem R, Blumenkopf T, Cory M, Elwell L, Moxham C, Ray P, et al. Expression and characterization of human pancreatic preprocarboxypeptidase A1 and preprocarboxypeptidase A2. Arch Biochem Biophys. 1996;332:8-18 pubmed..These experiments indicate that hCPA1 and hCPA2 are potential candidates for use in a human-based ADEPT approach. ..
- Klose T, Blaisdell J, Goldstein J. Gene structure of CYP2C8 and extrahepatic distribution of the human CYP2Cs. J Biochem Mol Toxicol. 1999;13:289-95 pubmed..Although CYP2C8 has been shown to be phenobarbital inducible, neither a barbiturate-responsive regulatory sequence (a Barbie box) nor a phenobarbital-responsive enhancer module (PBREM) was found within the upstream region analyzed. ..
- Hebbar P, Archer T. Nuclear factor 1 is required for both hormone-dependent chromatin remodeling and transcriptional activation of the mouse mammary tumor virus promoter. Mol Cell Biol. 2003;23:887-98 pubmed
- Knapp G, Setzer R, Fuscoe J. Quantitation of aberrant interlocus T-cell receptor rearrangements in mouse thymocytes and the effect of the herbicide 2,4-dichlorophenoxyacetic acid. Environ Mol Mutagen. 2003;42:37-43 pubmed..No significant increase in aberrant V(D)J rearrangements was found, indicating that under these conditions 2,4-D does not appear to effect this important mechanism of carcinogenesis. ..
- Bell D, Stephens E, Castranio T, Umbach D, Watson M, Deakin M, et al. Polyadenylation polymorphism in the acetyltransferase 1 gene (NAT1) increases risk of colorectal cancer. Cancer Res. 1995;55:3537-42 pubmed..12). This is the first study to test for cancer risk associated with the NAT1 gene, and these positive findings suggest that NAT1 alleles may be important genetic determinents of colorectal cancer risk. ..
- Risinger J, Umar A, Boyd J, Berchuck A, Kunkel T, Barrett J. Mutation of MSH3 in endometrial cancer and evidence for its functional role in heteroduplex repair. Nat Genet. 1996;14:102-5 pubmed
- Lombardo C, Consler T, Kassel D. In vitro phosphorylation of the epidermal growth factor receptor autophosphorylation domain by c-src: identification of phosphorylation sites and c-src SH2 domain binding sites. Biochemistry. 1995;34:16456-66 pubmed..The identification of c-src phosphorylation sequences on EGFR as c-src SH2 binding sites supports the notion that this interaction plays a significant role in the regulation of growth factor receptor function and signal transduction. ..
- Sweet D, Wolff N, Pritchard J. Expression cloning and characterization of ROAT1. The basolateral organic anion transporter in rat kidney. J Biol Chem. 1997;272:30088-95 pubmed..Each of these properties is identical to those previously described for the basolateral alpha-ketoglutarate/PAH exchanger in isolated membrane vesicles or intact renal tubules. ..
- Talarico T, Ray P, Dev I, Merrill B, Dallas W. Cloning, sequence analysis, and overexpression of Escherichia coli folK, the gene coding for 7,8-dihydro-6-hydroxymethylpterin-pyrophosphokinase. J Bacteriol. 1992;174:5971-7 pubmed..The enzyme was observed to have a large number of proline residues and migrated anomalously in sodium dodecyl sulfate-polyacrylamide gels, with an apparent molecular mass of 23,000 Da. ..
- Porter D, Chestnut W, Taylor L, Merrill B, Spector T. Inactivation of dihydropyrimidine dehydrogenase by 5-iodouracil. J Biol Chem. 1991;266:19988-94 pubmed..Fast atom bombardment mass spectral analysis of the smaller peptide yielded a protonated parent ion mass of 782 daltons that was consistent with X being a S-(hexahydro-2,4-dioxo-5-pyrimidinyl)cysteinyl residue. ..
- Lawton M, Gasser R, Tynes R, Hodgson E, Philpot R. The flavin-containing monooxygenase enzymes expressed in rabbit liver and lung are products of related but distinctly different genes. J Biol Chem. 1990;265:5855-61 pubmed..Analysis of genomic DNA indicates that both the hepatic and pulmonary enzymes may be products of single genes. ..
- Austin E, Huber B. A first step in the development of gene therapy for colorectal carcinoma: cloning, sequencing, and expression of Escherichia coli cytosine deaminase. Mol Pharmacol. 1993;43:380-7 pubmed..Growth inhibition studies showed a shift in the IC50 for 5-FC from 17,000 microM in the parental cell line to 30 microM in cells expressing CD. ..
- Sueyoshi T, Kawamoto T, Zelko I, Honkakoski P, Negishi M. The repressed nuclear receptor CAR responds to phenobarbital in activating the human CYP2B6 gene. J Biol Chem. 1999;274:6043-6 pubmed..Thus, activation of the repressed nuclear receptor CAR appears to be a versatile mediator that regulates PB induction of the CYP2B and other genes. ..
- Safrany S, Ingram S, Cartwright J, Falck J, McLennan A, Barnes L, et al. The diadenosine hexaphosphate hydrolases from Schizosaccharomyces pombe and Saccharomyces cerevisiae are homologues of the human diphosphoinositol polyphosphate phosphohydrolase. Overlapping substrate specificities in a MutT-type protein. J Biol Chem. 1999;274:21735-40 pubmed
- Kameda H, Risinger J, Han B, Baek S, Barrett J, Abe T, et al. Expression of Gab1 lacking the pleckstrin homology domain is associated with neoplastic progression. Mol Cell Biol. 2001;21:6895-905 pubmed publisher..These results demonstrate that Gab1 is a target of carcinogen-induced transformation of SHE cells and that the expression of a Gab1 variant lacking most of the PH domain plays a specific role in the neoplastic progression of SHE cells...
- Welch J, Barbee R, Magyar P, Bunch D, O Brien D. Expression of the spermatogenic cell-specific glyceraldehyde 3-phosphate dehydrogenase (GAPDS) in rat testis. Mol Reprod Dev. 2006;73:1052-60 pubmed..The current results are similar to those previously obtained for mouse GAPDS and human GAPD2, suggesting that reliable comparisons can be made between these species in toxicant screening and contraceptive development. ..
- Sharief F, Li S. Structure of human prostatic acid phosphatase gene. Biochem Biophys Res Commun. 1992;184:1468-76 pubmed..Southern blot analysis of ten mammalian genomic DNAs gave multiple EcoRI fragments. The data of human genomic DNAs were consistent with the total length of the PAP gene of at least 50 kilobases. ..
- Rosario M, Perkins S, O Brien D, Allen R, Eddy E. Identification of the gene for the developmentally expressed 70 kDa heat-shock protein (P70) of mouse spermatogenic cells. Dev Biol. 1992;150:1-11 pubmed..Two cDNAs were sequenced and found to be 99% homologous to the 3' end of the Hsp70.2 gene. These data strongly supported the hypothesis that P70 is the expressed product of the Hsp70.2 gene in mouse spermatogenic cells. ..
- Sudo K, Maekawa M, Ikawa S, Machida K, Kitamura M, Li S. A missense mutation found in human lactate dehydrogenase-B (H) variant gene. Biochem Biophys Res Commun. 1990;168:672-6 pubmed..This mutation results in an amino-acid replacement of a conserved arginine by histidine at the residue "173," which is involved in an anion-binding site at the P-axis of LDH subunits. ..
- Lindberg R, Burkhart B, Ichikawa T, Negishi M. The structure and characterization of type I P-450(15) alpha gene as major steroid 15 alpha-hydroxylase and its comparison with type II P-450(15) alpha gene. J Biol Chem. 1989;264:6465-71 pubmed..The results indicated that Type I was the major steroid 15 alpha-hydroxylase. The differential 15 alpha OH-1 expression, therefore, determined the sexual dimorphism of the tissue-specific 15 alpha-hydroxylase activity in mice. ..
- Lindberg R, Negishi M. Alteration of mouse cytochrome P450coh substrate specificity by mutation of a single amino-acid residue. Nature. 1989;339:632-4 pubmed
- Fulcher K, Mori C, Welch J, O Brien D, Klapper D, Eddy E. Characterization of Fsc1 cDNA for a mouse sperm fibrous sheath component. Biol Reprod. 1995;52:41-9 pubmed..abstract truncated at 250 words) ..
- Andrews K, Nettesheim P, Asai D, Ostrowski L. Identification of seven rat axonemal dynein heavy chain genes: expression during ciliated cell differentiation. Mol Biol Cell. 1996;7:71-9 pubmed..Identification of the mechanisms that regulate the cell-specific expression of these axonemal dynein heavy chains will further our understanding of the process of ciliated cell differentiation. ..
- Wine R, Ku W, Li L, Chapin R. Cyclophilin A is present in rat germ cells and is associated with spermatocyte apoptosis. Reproductive Toxicology Group. Biol Reprod. 1997;56:439-46 pubmed..These data demonstrate that rat testis germ cells contain relatively high levels of cyclophilin A whose nuclease activity is associated with spermatocyte apoptosis induced by 2-methoxyethanol. ..
- Potdar P, Andrews K, Nettesheim P, Ostrowski L. Expression and regulation of gamma-glutamyl transpeptidase-related enzyme in tracheal cells. Am J Physiol. 1997;273:L1082-9 pubmed..The induction of high levels of GGT-rel may play an important role in protecting the lung from oxidative stress or other toxic insults. ..
- Bennett L, Brownlee H, Hagavik S, Wiseman R. Sequence analysis of the rat Brca1 homolog and its promoter region. Mamm Genome. 1999;10:19-25 pubmed..Finally, analysis of splice variants from rat mammary gland, ovary, testis, spleen, and liver tissues revealed that, while alternative transcripts are detectable, full-length transcripts are the predominant steady-state form. ..
- Ghosh S, Moore S, Bell R, Dush M. Functional analysis of a phosphatidic acid binding domain in human Raf-1 kinase: mutations in the phosphatidate binding domain lead to tail and trunk abnormalities in developing zebrafish embryos. J Biol Chem. 2003;278:45690-6 pubmed..Our results demonstrate that a functional phosphatidate binding site is necessary for Raf-1 function in embryonic development. ..
- Wells M, Huang W, Li L, Gerrish K, Fargo D, Ozsolak F, et al. Posttranscriptional regulation of cell-cell interaction protein-encoding transcripts by Zfs1p in Schizosaccharomyces pombe. Mol Cell Biol. 2012;32:4206-14 pubmed..These data suggest that Zfs1p can both directly and indirectly regulate the levels of transcripts involved in cell-cell adhesion in this species. ..
- Vollberg T, Nervi C, George M, Fujimoto W, Krust A, Jetten A. Retinoic acid receptors as regulators of human epidermal keratinocyte differentiation. Mol Endocrinol. 1992;6:667-76 pubmed..These results demonstrate that the control of NHEK differentiation by RA is consistent with the interaction of the retinoid with RAR and the regulation of transcription by that ligand-receptor complex. ..
- Jahnke G, Chao J, Walker M, DiAugustine R. Detection of a kallikrein in the mouse lactating mammary gland: a possible processing enzyme for the epidermal growth factor precursor. Endocrinology. 1994;135:2022-9 pubmed..These results support the hypothesis that the release of EGF-immunoreactive material into the milk is in part dependent upon a kallikrein enzyme (mGK-6) in the BALB/c mouse lactating mammary gland. ..
- Nolte R, Wisely G, Westin S, Cobb J, Lambert M, Kurokawa R, et al. Ligand binding and co-activator assembly of the peroxisome proliferator-activated receptor-gamma. Nature. 1998;395:137-43 pubmed
- Porter D, Chestnut W, Merrill B, Spector T. Mechanism-based inactivation of dihydropyrimidine dehydrogenase by 5-ethynyluracil. J Biol Chem. 1992;267:5236-42 pubmed..5-Cyanouracil-inactivated enzyme slowly regained activity (t1/2 = 1.8 min) after dilution into the standard assay. DHPDHases isolated from rat, mouse, and human liver had similar sensitivities to inactivation by 5-alkynyluracils. ..
- Hiraoka B, Sharief F, Yang Y, Li W, Li S. The cDNA and protein sequences of mouse lactate dehydrogenase B. Molecular evolution of vertebrate lactate dehydrogenase genes A (muscle), B (heart) and C (testis). Eur J Biochem. 1990;189:215-20 pubmed..In agreement with the previous results, this analysis further indicates that lactate dehydrogenase-C gene branched off earlier than did lactate dehydrogenase-A and lactate dehydrogenase-B genes. ..
- Frothingham R, Meeker O Connell W, Talbot E, George J, Kreuzer K. Identification, cloning, and expression of the Escherichia coli pyrazinamidase and nicotinamidase gene, pncA. Antimicrob Agents Chemother. 1996;40:1426-31 pubmed..coli cannot. We conclude that a single E. coli enzyme accounts for both PZAase and nicotinamidase activities and that ORF1 is the E.coli PZAase and nicotinamidase gene, pncA. ..