Experts and Doctors on g protein coupled receptors in Pennsylvania, United States
Locale: Pennsylvania, United States
Topic: g protein coupled receptors
Ferrer M, Kolodin G, Zuck P, Peltier R, Berry K, Mandala S, et al
. A fully automated [35S]GTPgammaS scintillation proximity assay for the high-throughput screening of Gi-linked G protein-coupled receptors. Assay Drug Dev Technol. 2003;1:261-73 pubmed
..Here we compare [(35)S]GTPgammaS scintillation proximity binding assays for two different G(i)-coupled GPCRs, and describe their implementation with automated high-throughput systems. ..
Lawson E, Luci D, Ghosh S, Kinney W, Reynolds C, Qi J, et al
. Nonpeptide urotensin-II receptor antagonists: a new ligand class based on piperazino-phthalimide and piperazino-isoindolinone subunits. J Med Chem. 2009;52:7432-45 pubmed publisher
..A discussion of U-II receptor antagonist pharmacophores is presented, and a specifically defined model is suggested from tricycle 13, which has a high degree of conformational constraint. ..
Cox C, Breslin M, Whitman D, Schreier J, McGaughey G, Bogusky M, et al
. Discovery of the dual orexin receptor antagonist [(7R)-4-(5-chloro-1,3-benzoxazol-2-yl)-7-methyl-1,4-diazepan-1-yl][5-methyl-2-(2H-1,2,3-triazol-2-yl)phenyl]methanone (MK-4305) for the treatment of insomnia. J Med Chem. 2010;53:5320-32 pubmed publisher
Kennerdell J, Carthew R. Use of dsRNA-mediated genetic interference to demonstrate that frizzled and frizzled 2 act in the wingless pathway. Cell. 1998;95:1017-26 pubmed
..Our results demonstrate that dsRNA interference can be used to analyze many aspects of gene function. ..
Clements M, McDonald T, Wang R, Xie G, O Dowd B, George S, et al
. FMRFamide-related neuropeptides are agonists of the orphan G-protein-coupled receptor GPR54. Biochem Biophys Res Commun. 2001;284:1189-93 pubmed
..Northern analysis of human GPR54 detected expression in several peripheral tissues and many regions of the central nervous system. ..
Athanasiadis A, Rich A, Maas S. Widespread A-to-I RNA editing of Alu-containing mRNAs in the human transcriptome. PLoS Biol. 2004;2:e391 pubmed
..The analysis suggests that modification of repetitive elements is a predominant activity for RNA editing with significant implications for cellular gene expression. ..
Xin H, Wang Y, Todd M, Qi J, Minor L. Evaluation of no-wash calcium assay kits: enabling tools for calcium mobilization. J Biomol Screen. 2007;12:705-14 pubmed
Rominger C, Bee W, Copeland R, Davenport E, Gilmartin A, Gontarek R, et al
. Evidence for allosteric interactions of antagonist binding to the smoothened receptor. J Pharmacol Exp Ther. 2009;329:995-1005 pubmed publisher
..Our mechanism of action characterization of radioligand binding to Smo combined with functional data provides a better understanding of small-molecule interactions with Smo and their influence on the Hh pathway...
Zhang G, Chen T, Connolly B, Lin S, Hargreaves R, Vanko A, et al
. In vivo optical imaging of LacZ expression using lacZ transgenic mice. Assay Drug Dev Technol. 2009;7:391-9 pubmed publisher
..Thus, this technology can potentially be used to noninvasively image changes of certain endogenous molecules and/or molecular pathways in transgenic animals. ..