Experts and Doctors on fungal proteins in Connecticut, United States


Locale: Connecticut, United States
Topic: fungal proteins

Top Publications

  1. Smith A, Roeder G. The yeast Red1 protein localizes to the cores of meiotic chromosomes. J Cell Biol. 1997;136:957-67 pubmed
    ..These observations are consistent with previous genetic studies suggesting that Red1 and Hop1 directly interact. There is little or no Hop1 protein on pachytene chromosomes or in synapsed chromosomal regions. ..
  2. Fiebig K, Rice L, Pollock E, Brunger A. Folding intermediates of SNARE complex assembly. Nat Struct Biol. 1999;6:117-23 pubmed
    ..Our data suggest a directed assembly process beginning distal to the membrane surfaces and proceeding toward them, bringing membranes into close proximity and possibly leading to membrane fusion. ..
  3. Yoo C, Wolin S. La proteins from Drosophila melanogaster and Saccharomyces cerevisiae: a yeast homolog of the La autoantigen is dispensable for growth. Mol Cell Biol. 1994;14:5412-24 pubmed
  4. Dohlman H, Song J, Ma D, Courchesne W, Thorner J. Sst2, a negative regulator of pheromone signaling in the yeast Saccharomyces cerevisiae: expression, localization, and genetic interaction and physical association with Gpa1 (the G-protein alpha subunit). Mol Cell Biol. 1996;16:5194-209 pubmed
    ..These results demonstrate that Sst2 and Gpa1 interact physically and suggest that Sst2 is a direct negative regulator of Gpa1. ..
  5. DiBello P, Garrison T, Apanovitch D, Hoffman G, Shuey D, Mason K, et al. Selective uncoupling of RGS action by a single point mutation in the G protein alpha-subunit. J Biol Chem. 1998;273:5780-4 pubmed
    ..The Galphasst mutant will be useful for uncoupling RGS-mediated regulation from other modes of signal regulation in whole cells and animals. ..
  6. Abeliovich H, Grote E, Novick P, Ferro Novick S. Tlg2p, a yeast syntaxin homolog that resides on the Golgi and endocytic structures. J Biol Chem. 1998;273:11719-27 pubmed
    ..Based on these findings we propose that Tlg2p is a t-SNARE that functions in transport from the endosome to the late Golgi and on the endocytic pathway. ..
  7. Sheu Y, Santos B, Fortin N, Costigan C, Snyder M. Spa2p interacts with cell polarity proteins and signaling components involved in yeast cell morphogenesis. Mol Cell Biol. 1998;18:4053-69 pubmed
    ..We thus propose that Spa2p, Pea2p, and Bud6p function together, perhaps as a complex, to promote polarized morphogenesis through regulation of the actin cytoskeleton and signaling pathways. ..
  8. Roth D, Guo W, Novick P. Dominant negative alleles of SEC10 reveal distinct domains involved in secretion and morphogenesis in yeast. Mol Biol Cell. 1998;9:1725-39 pubmed
  9. Barral Y, Mermall V, Mooseker M, Snyder M. Compartmentalization of the cell cortex by septins is required for maintenance of cell polarity in yeast. Mol Cell. 2000;5:841-51 pubmed
    ..We propose that septins maintain cell polarity by specifying a boundary between cortical domains. ..

More Information

Publications126 found, 100 shown here

  1. Dohlman H, Thorner J. Regulation of G protein-initiated signal transduction in yeast: paradigms and principles. Annu Rev Biochem. 2001;70:703-54 pubmed
  2. Casamayor A, Snyder M. Molecular dissection of a yeast septin: distinct domains are required for septin interaction, localization, and function. Mol Cell Biol. 2003;23:2762-77 pubmed
    ..Thus, we find that septins are multifunctional proteins with specific domains involved in distinct molecular interactions required for assembly, localization, and function within the cell. ..
  3. Snyder M. The SPA2 protein of yeast localizes to sites of cell growth. J Cell Biol. 1989;108:1419-29 pubmed
    ..Thus, SPA2 is a newly identified yeast gene that is involved in the direction and control of cell division, and whose gene product localizes to the site of cell growth. ..
  4. Jiang Y, Rossi G, Ferro Novick S. Bet2p and Mad2p are components of a prenyltransferase that adds geranylgeranyl onto Ypt1p and Sec4p. Nature. 1993;366:84-6 pubmed
    ..The efficient transfer of geranylgeranyl onto small GTP-binding proteins requires the presence of an additional activity. ..
  5. Wertz J, Riley M. Chimeric nature of two plasmids of Hafnia alvei encoding the bacteriocins alveicins A and B. J Bacteriol. 2004;186:1598-605 pubmed
    ..alvei population relatively rapidly...
  6. Cheng M, Hartl F, Martin J, Pollock R, Kalousek F, Neupert W, et al. Mitochondrial heat-shock protein hsp60 is essential for assembly of proteins imported into yeast mitochondria. Nature. 1989;337:620-5 pubmed
    ..hsp60 is a member of the 'chaperonin' class of protein factors, which include the Escherichia coli groEL protein and the Rubisco subunit-binding protein of chloroplasts. ..
  7. Rockmill B, Sym M, Scherthan H, Roeder G. Roles for two RecA homologs in promoting meiotic chromosome synapsis. Genes Dev. 1995;9:2684-95 pubmed
    ..In a zip1 haploid, axial associations are not apparent, suggesting that these associations depend on interactions between homologous sequences. ..
  8. Costigan C, Kolodrubetz D, Snyder M. NHP6A and NHP6B, which encode HMG1-like proteins, are candidates for downstream components of the yeast SLT2 mitogen-activated protein kinase pathway. Mol Cell Biol. 1994;14:2391-403 pubmed
    ..Our results indicate that the Slt2p MAPK pathway in Saccharomyces cerevisiae may mediate its function in cell growth and morphogenesis, at least in part, through high-mobility group proteins. ..
  9. Song J, Dohlman H. Partial constitutive activation of pheromone responses by a palmitoylation-site mutant of a G protein alpha subunit in yeast. Biochemistry. 1996;35:14806-17 pubmed
    ..Taken together, our data suggest that Gpa1p is palmitoylated via a thioester bond at Cys 3 and that palmitoylation plays a role in modulating Gpa1p signaling and membrane localization. ..
  10. Chua P, Roeder G. Zip2, a meiosis-specific protein required for the initiation of chromosome synapsis. Cell. 1998;93:349-59 pubmed
    ..We propose that Zip2p promotes the initiation of chromosome synapsis and that localization of Zip2p to sites of interhomolog recombination ensures synapsis between homologous chromosomes...
  11. Leu J, Roeder G. The pachytene checkpoint in S. cerevisiae depends on Swe1-mediated phosphorylation of the cyclin-dependent kinase Cdc28. Mol Cell. 1999;4:805-14 pubmed
    ..The requirement for Swe1 distinguishes the pachytene checkpoint from the DNA damage checkpoints operating in vegetative cells. ..
  12. Grote E, Carr C, Novick P. Ordering the final events in yeast exocytosis. J Cell Biol. 2000;151:439-52 pubmed
    ..In the sec18-1 mutant, cis-SNARE complexes containing surface-accessible Sncp accumulate in the plasma membrane. Thus, one function of Sec18p is to disassemble SNARE complexes on the postfusion membrane. ..
  13. Trieu M, Devine E, Lamarche L, Ammerman A, Greco J, Birge R, et al. Expression, purification, and spectral tuning of RhoGC, a retinylidene/guanylyl cyclase fusion protein and optogenetics tool from the aquatic fungus Blastocladiella emersonii. J Biol Chem. 2017;292:10379-10389 pubmed publisher
    ..We anticipate that this expression/purification system and these RhoGC mutants will facilitate mechanistic and structural exploration of this important enzyme. ..
  14. Grabińska K, Edani B, Park E, Kraehling J, Sessa W. A conserved C-terminal RXG motif in the NgBR subunit of cis-prenyltransferase is critical for prenyltransferase activity. J Biol Chem. 2017;292:17351-17361 pubmed publisher
    ..The strong conservation of the RXG motif among NgBR orthologs indicates that this subunit is critical for the synthesis of polyprenol diphosphates and cellular function. ..
  15. Zheng P, Fay D, Burton J, Xiao H, Pinkham J, Stern D. SPK1 is an essential S-phase-specific gene of Saccharomyces cerevisiae that encodes a nuclear serine/threonine/tyrosine kinase. Mol Cell Biol. 1993;13:5829-42 pubmed
  16. Jiang Y, Scarpa A, Zhang L, Stone S, Feliciano E, Ferro Novick S. A high copy suppressor screen reveals genetic interactions between BET3 and a new gene. Evidence for a novel complex in ER-to-Golgi transport. Genetics. 1998;149:833-41 pubmed
    ..The identification of mammalian homologues of Bet3p and Bet5p implies that the Bet3p/Bet5p complex is highly conserved in evolution. ..
  17. Costigan C, Gehrung S, Snyder M. A synthetic lethal screen identifies SLK1, a novel protein kinase homolog implicated in yeast cell morphogenesis and cell growth. Mol Cell Biol. 1992;12:1162-78 pubmed
    ..Tatchell, Mol. Cell. Biol. 11:3369-3373, 1991). These data suggest that SLK1 plays a role in both cell morphogenesis and the control of cell growth. We speculate that SLK1 may be a regulatory link for these two cellular processes. ..
  18. Newman A, Ferro Novick S. Characterization of new mutants in the early part of the yeast secretory pathway isolated by a [3H]mannose suicide selection. J Cell Biol. 1987;105:1587-94 pubmed
    ..This and previous findings (Novick, P., C. Field, and R. Schekman, 1980, Cell, 21:205-215) suggest that protein exit from the ER and entry into the Golgi apparatus is a complex process requiring at least 11 genes. ..
  19. Page B, Satterwhite L, Rose M, Snyder M. Localization of the Kar3 kinesin heavy chain-related protein requires the Cik1 interacting protein. J Cell Biol. 1994;124:507-19 pubmed
    ..These data indicate that interaction between a putative kinesin heavy chain-related protein and another protein can determine the localization of motor activity and thereby affect the functional specificity of the motor complex. ..
  20. Guimarães C, Udier Blagovic M, Jorgensen W. Macrophomate synthase: QM/MM simulations address the Diels-Alder versus Michael-Aldol reaction mechanism. J Am Chem Soc. 2005;127:3577-88 pubmed
    ..2004, D60, 1187-1197). A modified link-atom treatment for the bonds at the QM/MM interface is also presented. ..
  21. Dohlman H, Apaniesk D, Chen Y, Song J, Nusskern D. Inhibition of G-protein signaling by dominant gain-of-function mutations in Sst2p, a pheromone desensitization factor in Saccharomyces cerevisiae. Mol Cell Biol. 1995;15:3635-43 pubmed
    ..These results point to the G protein alpha subunit as being the direct target of Sst2p action and underscore the importance of this novel desensitization factor in G-protein-mediated signaling. ..
  22. Roemer T, Madden K, Chang J, Snyder M. Selection of axial growth sites in yeast requires Axl2p, a novel plasma membrane glycoprotein. Genes Dev. 1996;10:777-93 pubmed
    ..We suggest that Axl2p acts as an anchor in the plasma membrane that helps direct new growth components and/or polarity establishment components to the cortical axial budding site. ..
  23. Walch Solimena C, Collins R, Novick P. Sec2p mediates nucleotide exchange on Sec4p and is involved in polarized delivery of post-Golgi vesicles. J Cell Biol. 1997;137:1495-509 pubmed
    ..We propose that Sec2p functions to couple the activation of Sec4p to the polarized delivery of vesicles to the site of exocytosis. ..
  24. Erdman S, Lin L, Malczynski M, Snyder M. Pheromone-regulated genes required for yeast mating differentiation. J Cell Biol. 1998;140:461-83 pubmed
    ..Our results indicate that a variety of novel genes are expressed specifically during mating differentiation to mediate proper cell morphogenesis, cell fusion, and other steps of the mating process. ..
  25. Tung K, Roeder G. Meiotic chromosome morphology and behavior in zip1 mutants of Saccharomyces cerevisiae. Genetics. 1998;149:817-32 pubmed
    ..In contrast, the role of Zip1p in synapsis is separable from its role in meiotic cell cycle progression. zip1 mutants display interval-specific effects on crossing over. ..
  26. Dong H, Roeder G. Organization of the yeast Zip1 protein within the central region of the synaptonemal complex. J Cell Biol. 2000;148:417-26 pubmed
    ..Together, these results suggest that two Zip1 dimers, lying head-to-head, span the width of the SC. ..
  27. Santos B, Snyder M. Sbe2p and sbe22p, two homologous Golgi proteins involved in yeast cell wall formation. Mol Biol Cell. 2000;11:435-52 pubmed
    ..Thus, we suggest a model in which Sbe2p and Sbe22p are involved in the transport of cell wall components from the Golgi apparatus to the cell surface periphery in a pathway independent of Chs5p. ..
  28. Agarwal S, Roeder G. Zip3 provides a link between recombination enzymes and synaptonemal complex proteins. Cell. 2000;102:245-55 pubmed
    ..We speculate that Zip3 is a component of recombination nodules and serves to link the initiation of synapsis to meiotic recombination...
  29. San Segundo P, Roeder G. Role for the silencing protein Dot1 in meiotic checkpoint control. Mol Biol Cell. 2000;11:3601-15 pubmed
    ..In vegetative cells, mutation of DOT1 results in delocalization of Sir3 from telomeres, accounting for the impaired telomeric silencing in dot1. ..
  30. Devore Carter D, Kar S, Vellucci V, Bhattacherjee V, Domanski P, Hostetter M. Superantigen-like effects of a Candida albicans polypeptide. J Infect Dis. 2008;197:981-9 pubmed publisher
    ..Histidine(263) was required. Pep(263) bound to T lymphocytes and MHC class II and was detected in the urine of a patient with C. albicans fungemia. These studies identify a candidal protein that displays superantigen-like activities...
  31. Terbush D, Novick P. Sec6, Sec8, and Sec15 are components of a multisubunit complex which localizes to small bud tips in Saccharomyces cerevisiae. J Cell Biol. 1995;130:299-312 pubmed
    ..The c-myc-Sec8 protein is localized by immunofluorescence to small bud tips indicating that the Sec6/8/15 complex may function at sites of exocytosis. ..
  32. Collins R, Brennwald P, Garrett M, Lauring A, Novick P. Interactions of nucleotide release factor Dss4p with Sec4p in the post-Golgi secretory pathway of yeast. J Biol Chem. 1997;272:18281-9 pubmed
    ..We examined the relationship of GDI1 and DSS4 with SEC4 both genetically and biochemically. These results exclude a role for DSS4 in the recruitment of Sec4p/GDI onto membranes. ..
  33. Gillen K, Pausch M, Dohlman H. N-terminal domain of Gpa1 (G protein alpha) subunit) is sufficient for plasma membrane targeting in yeast Saccharomyces cerevisiae. J Cell Sci. 1998;111 ( Pt 21):3235-44 pubmed
    ..This analysis defines the extreme N terminus of Gpa1 as the primary determinant of proper membrane targeting, and represents an essential step towards isolating and identifying G protein-targeting proteins within the plasma membrane. ..
  34. Kim D, Sacher M, Scarpa A, Quinn A, Ferro Novick S. High-copy suppressor analysis reveals a physical interaction between Sec34p and Sec35p, a protein implicated in vesicle docking. Mol Biol Cell. 1999;10:3317-29 pubmed
    ..Sec34p and Sec35p stably associate with each other to form a multiprotein complex of approximately 480 kDa. These data indicate that Sec34p acts in conjunction with Sec35p to mediate a common step in vesicular traffic. ..
  35. Burton J, Solomon M. D box and KEN box motifs in budding yeast Hsl1p are required for APC-mediated degradation and direct binding to Cdc20p and Cdh1p. Genes Dev. 2001;15:2381-95 pubmed
    ..These results indicate that D box and KEN box motifs are important for direct binding to the APC machinery, leading to ubiquitination and subsequent protein degradation. ..
  36. Du L, Novick P. Pag1p, a novel protein associated with protein kinase Cbk1p, is required for cell morphogenesis and proliferation in Saccharomyces cerevisiae. Mol Biol Cell. 2002;13:503-14 pubmed
    ..Pag1p is a conserved protein. The homologs of Pag1p in other organisms are likely to form complexes with the Cbk1p-related kinases and function with those kinases in the same biological processes. ..
  37. Page B, Snyder M. CIK1: a developmentally regulated spindle pole body-associated protein important for microtubule functions in Saccharomyces cerevisiae. Genes Dev. 1992;6:1414-29 pubmed
  38. Turi T, Webster P, Rose J. Brefeldin A sensitivity and resistance in Schizosaccharomyces pombe. Isolation of multiple genes conferring resistance. J Biol Chem. 1994;269:24229-36 pubmed
    ..The overexpression of pap1 probably confers BFA resistance indirectly by inducing expression of one or more other proteins. The isolation of several genes conferring BFA resistance suggests several mechanisms are involved. ..
  39. Apanovitch D, Slep K, Sigler P, Dohlman H. Sst2 is a GTPase-activating protein for Gpa1: purification and characterization of a cognate RGS-Galpha protein pair in yeast. Biochemistry. 1998;37:4815-22 pubmed
    ..These experiments represent the first biochemical characterization of Gpa1 and Sst2, and provide a molecular basis for their well-established biological roles in signaling and desensitization. ..
  40. Bidlingmaier S, Weiss E, Seidel C, Drubin D, Snyder M. The Cbk1p pathway is important for polarized cell growth and cell separation in Saccharomyces cerevisiae. Mol Cell Biol. 2001;21:2449-62 pubmed
    ..Many Cbk1-related kinases have been shown to regulate cellular morphology. ..
  41. Santos B, Snyder M. Specific protein targeting during cell differentiation: polarized localization of Fus1p during mating depends on Chs5p in Saccharomyces cerevisiae. Eukaryot Cell. 2003;2:821-5 pubmed
    ..Here we show that Chs5p is also essential for the polarized targeting of Fus1p, but not of other cell fusion proteins, to the membrane during mating. ..
  42. Tsubouchi H, Roeder G. Budding yeast Hed1 down-regulates the mitotic recombination machinery when meiotic recombination is impaired. Genes Dev. 2006;20:1766-75 pubmed
    ..We propose that Hed1 provides a mechanism to ensure the coordinated action of Rad51 and Dmc1 during meiosis, by down-regulating Rad51 activity when Dmc1 is unavailable. ..
  43. Rockmill B, Engebrecht J, Scherthan H, Loidl J, Roeder G. The yeast MER2 gene is required for chromosome synapsis and the initiation of meiotic recombination. Genetics. 1995;141:49-59 pubmed
    ..We suggest that the primary defect in the mer2 mutant is in the initiation of meiotic genetic exchange. ..
  44. Cheng A, Ross K, Kaldis P, Solomon M. Dephosphorylation of cyclin-dependent kinases by type 2C protein phosphatases. Genes Dev. 1999;13:2946-57 pubmed
    ..Furthermore, PP2C-like enzymes are the predominant phosphatases toward human Cdk2 in HeLa cell extracts, indicating that the substrate specificity of PP2Cs toward CDKs is evolutionarily conserved. ..
  45. Novak J, Ross MacDonald P, Roeder G. The budding yeast Msh4 protein functions in chromosome synapsis and the regulation of crossover distribution. Genetics. 2001;158:1013-25 pubmed
    ..Both Zip2 and Zip1 are required for the normal localization of Msh4 to chromosomes, raising the possibility that the zip1 and zip2 defects in crossing over are indirect, resulting from the failure to localize Msh4 properly. ..
  46. Juo Z, Kassavetis G, Wang J, Geiduschek E, Sigler P. Crystal structure of a transcription factor IIIB core interface ternary complex. Nature. 2003;422:534-9 pubmed
    ..The structure reveals the core interface for assembly of TFIIIB and demonstrates how the loosely packed Brf1 domain achieves remarkable binding specificity with the convex and lateral surfaces of TBP. ..
  47. Hooker G, Roeder G. A Role for SUMO in meiotic chromosome synapsis. Curr Biol. 2006;16:1238-43 pubmed
    ..The ubc9 mutant phenotype provides the first evidence for a cause-and-effect relationship between sumoylation and synapsis...
  48. Horwich A, Willison K. Protein folding in the cell: functions of two families of molecular chaperone, hsp 60 and TF55-TCP1. Philos Trans R Soc Lond B Biol Sci. 1993;339:313-25; discussion 325-6 pubmed
    ..We review here studies of both hsp 60 and other family members, discussing their essential physiological roles and mechanism of action. ..
  49. Flescher E, Madden K, Snyder M. Components required for cytokinesis are important for bud site selection in yeast. J Cell Biol. 1993;122:373-86 pubmed
    ..e., critical components at the site of cytokinesis are involved in determining the next site of polarized growth and division. ..
  50. Moya M, Roberts D, Novick P. DSS4-1 is a dominant suppressor of sec4-8 that encodes a nucleotide exchange protein that aids Sec4p function. Nature. 1993;361:460-3 pubmed
    ..We report here the use of suppressor analysis to clone a gene, dss4, encoding a 17K protein that aids Sec4p action in vivo by functioning as a GDP dissociation stimulator. ..
  51. Rossi G, Kolstad K, Stone S, Palluault F, Ferro Novick S. BET3 encodes a novel hydrophilic protein that acts in conjunction with yeast SNAREs. Mol Biol Cell. 1995;6:1769-80 pubmed
    ..These findings support the hypothesis that Bet3p may act before the assembly of the SNARE complex. ..
  52. Carr C, Grote E, Munson M, Hughson F, Novick P. Sec1p binds to SNARE complexes and concentrates at sites of secretion. J Cell Biol. 1999;146:333-44 pubmed
    ..The results presented here place yeast Sec1p at the core of the exocytic fusion machinery, bound to SNARE complexes and localized to sites of secretion. ..
  53. Bailis J, Roeder G. Pachytene exit controlled by reversal of Mek1-dependent phosphorylation. Cell. 2000;101:211-21 pubmed
    ..We speculate that phosphorylated Red1 prevents exit from pachytene and that completion of meiotic recombination triggers Glc7-dependent dephosphorylation of Red1. ..
  54. Kreft S, Wang L, Hochstrasser M. Membrane topology of the yeast endoplasmic reticulum-localized ubiquitin ligase Doa10 and comparison with its human ortholog TEB4 (MARCH-VI). J Biol Chem. 2006;281:4646-53 pubmed
    ..Finally, we provide evidence that the likely human Doa10 ortholog, TEB4 (MARCH-VI), adopts a topology similar to that of Doa10...
  55. Kaldis P, Sutton A, Solomon M. The Cdk-activating kinase (CAK) from budding yeast. Cell. 1996;86:553-64 pubmed
    ..Our data raise the intriguing possibility that p40MO15-cyclin H-MAT1, identified as the predominant CAK in vertebrate cell extracts, may not function as a physiological CAK. ..
  56. Burns N, Grimwade B, Ross MacDonald P, Choi E, Finberg K, Roeder G, et al. Large-scale analysis of gene expression, protein localization, and gene disruption in Saccharomyces cerevisiae. Genes Dev. 1994;8:1087-105 pubmed
    ..Our results will allow researchers who identify a yeast gene to determine immediately whether that gene is expressed at a specific time during the life cycle and whether its gene product localizes to a specific subcellular location. ..
  57. Santos B, Snyder M. Targeting of chitin synthase 3 to polarized growth sites in yeast requires Chs5p and Myo2p. J Cell Biol. 1997;136:95-110 pubmed
    ..We suggest a model in which Chs3p polarization to cortical sites in yeast is dependent on both Chs5p and the actin cytoskeleton/Myo2p. ..
  58. Madden K, Snyder M. Cell polarity and morphogenesis in budding yeast. Annu Rev Microbiol. 1998;52:687-744 pubmed
    ..Since many of the components important for polarized cell growth are conserved in other organisms, the basic mechanisms mediating polarized cell growth are likely to be universal among eukaryotes. ..
  59. Grote E, Vlacich G, Pypaert M, Novick P. A snc1 endocytosis mutant: phenotypic analysis and suppression by overproduction of dihydrosphingosine phosphate lyase. Mol Biol Cell. 2000;11:4051-65 pubmed
  60. Guo W, Tamanoi F, Novick P. Spatial regulation of the exocyst complex by Rho1 GTPase. Nat Cell Biol. 2001;3:353-60 pubmed
    ..These results reveal the action of parallel pathways for the polarized localization of the exocytic machinery, both of which are under the control of Rho1, a master regulator of cell polarity. ..
  61. Ni L, Snyder M. A genomic study of the bipolar bud site selection pattern in Saccharomyces cerevisiae. Mol Biol Cell. 2001;12:2147-70 pubmed
    ..Genome-wide screens of defined collections of mutants hold significant promise for dissecting many biological processes in yeast. ..
  62. Wehner K, Gallagher J, Baserga S. Components of an interdependent unit within the SSU processome regulate and mediate its activity. Mol Cell Biol. 2002;22:7258-67 pubmed
    ..Furthermore, heterologous complementation experiments demonstrate that the last 95 amino acids of yeast Mpp10p are specifically required for growth and pre-rRNA processing at low temperatures. ..
  63. Lee S, Schwartz M, Duong J, Stern D. Rad53 phosphorylation site clusters are important for Rad53 regulation and signaling. Mol Cell Biol. 2003;23:6300-14 pubmed
    ..Thus, the phosphorylation of Rad53 by upstream kinases is important not only for Rad53 activation but also for creation of an interface between Rad53 and Dun1. ..
  64. Ferris H, Furukawa Y, Minamino T, Kroetz M, Kihara M, Namba K, et al. FlhB regulates ordered export of flagellar components via autocleavage mechanism. J Biol Chem. 2005;280:41236-42 pubmed
    ..Finally, we provide evidence via peptide analysis and FlhB cleavage variants that the tertiary structure of FlhB plays a significant role in cleavage. Based on these results, we propose that FlhB cleavage is an autocatalytic process. ..
  65. Wang Z, Lopez Giraldez F, Lehr N, Farré M, Common R, Trail F, et al. Global gene expression and focused knockout analysis reveals genes associated with fungal fruiting body development in Neurospora crassa. Eukaryot Cell. 2014;13:154-69 pubmed publisher
    ..Overall, our analysis provides a fine-scale transcriptomic landscape and novel inferences regarding the control of the multistage development process of sexual crossing and fruiting body development in N. crassa. ..
  66. Berbasova T, Nallur S, Sells T, Smith K, Gordon P, Tausta S, et al. Fluoride export (FEX) proteins from fungi, plants and animals are 'single barreled' channels containing one functional and one vestigial ion pore. PLoS ONE. 2017;12:e0177096 pubmed publisher
    ..These data suggest that FEX family of fluoride channels in eukaryotes are 'single-barreled' transporters containing one functional pore and a second non-functional vestigial remnant of a homologous gene fusion event. ..
  67. Kabcenell A, Goud B, Northup J, Novick P. Binding and hydrolysis of guanine nucleotides by Sec4p, a yeast protein involved in the regulation of vesicular traffic. J Biol Chem. 1990;265:9366-72 pubmed
    ..0012 min-1. By analogy with other classes of GTP binding proteins, both the nucleotide exchange and hydrolysis activities of Sec4p may be modulated in vivo to facilitate its role in the regulation of intercompartmental membrane traffic. ..
  68. Govindan B, Bowser R, Novick P. The role of Myo2, a yeast class V myosin, in vesicular transport. J Cell Biol. 1995;128:1055-68 pubmed
    ..Our observations are consistent with a role for Myo2 in transporting a class of secretory vesicles from the mother cell along actin cables into the bud. ..
  69. Song J, Hirschman J, Gunn K, Dohlman H. Regulation of membrane and subunit interactions by N-myristoylation of a G protein alpha subunit in yeast. J Biol Chem. 1996;271:20273-83 pubmed
    ..These data suggest that myristoylation is required for specific targeting of Gpa1p to the plasma membrane, where it is needed to interact with the receptor and to regulate the release of Gbetagamma. ..
  70. Hong E, Roeder G. A role for Ddc1 in signaling meiotic double-strand breaks at the pachytene checkpoint. Genes Dev. 2002;16:363-76 pubmed
    ..Ddc1 therefore appears to operate in a positive feedback loop that promotes Mek1 function. ..
  71. Bruno V, Wang Z, Marjani S, Euskirchen G, Martin J, Sherlock G, et al. Comprehensive annotation of the transcriptome of the human fungal pathogen Candida albicans using RNA-seq. Genome Res. 2010;20:1451-8 pubmed publisher
    ..albicans, a necessary framework for a complete understanding of the molecular mechanisms of pathogenesis for this important eukaryotic pathogen. ..
  72. Garcia E, McPherson P, Chilcote T, Takei K, De Camilli P. rbSec1A and B colocalize with syntaxin 1 and SNAP-25 throughout the axon, but are not in a stable complex with syntaxin. J Cell Biol. 1995;129:105-20 pubmed
    ..They also suggest that the function of rbSec1, syntaxin 1, and SNAP-25 is not restricted to synaptic vesicle exocytosis at the synapse. ..
  73. Lian J, Stone S, Jiang Y, Lyons P, Ferro Novick S. Ypt1p implicated in v-SNARE activation. Nature. 1994;372:698-701 pubmed
    ..Because the v-SNARE resides on more than one membrane, such a regulated activation step may be necessary to prevent the premature fusion of donor and acceptor compartments. ..
  74. Manning B, Barrett J, Wallace J, Granok H, Snyder M. Differential regulation of the Kar3p kinesin-related protein by two associated proteins, Cik1p and Vik1p. J Cell Biol. 1999;144:1219-33 pubmed
    ..Our results indicate that Kar3p forms functionally distinct complexes with Cik1p and Vik1p to participate in different microtubule-mediated events within the same cell. ..
  75. Sheu Y, Barral Y, Snyder M. Polarized growth controls cell shape and bipolar bud site selection in Saccharomyces cerevisiae. Mol Cell Biol. 2000;20:5235-47 pubmed
    ..We propose that apical growth and repolarization at the site of cytokinesis are crucial for establishing spatial cues used by diploid yeast cells to position division planes. ..
  76. Finger F, Novick P. Synthetic interactions of the post-Golgi sec mutations of Saccharomyces cerevisiae. Genetics. 2000;156:943-51 pubmed
    ..The significance of these results is discussed in the context of both secretory pathway function and the utility of synthetic lethality studies and their interpretation. ..
  77. Rockmill B, Voelkel Meiman K, Roeder G. Centromere-proximal crossovers are associated with precocious separation of sister chromatids during meiosis in Saccharomyces cerevisiae. Genetics. 2006;174:1745-54 pubmed
    ..We present a model for PSSC in which a centromere-proximal CO promotes local loss of sister-chromatid cohesion. ..
  78. Tsubouchi T, MacQueen A, Roeder G. Initiation of meiotic chromosome synapsis at centromeres in budding yeast. Genes Dev. 2008;22:3217-26 pubmed publisher
    ..Our data provide evidence for two classes of synapsis initiation events that differ in location, timing, genetic requirements, and relationship to meiotic recombination...
  79. Bowser R, Muller H, Govindan B, Novick P. Sec8p and Sec15p are components of a plasma membrane-associated 19.5S particle that may function downstream of Sec4p to control exocytosis. J Cell Biol. 1992;118:1041-56 pubmed
    ..We propose that this particle may function as a downstream effector of Sec4p, serving to direct the fusion of secretory vesicles with the plasma membrane. ..
  80. Fortini M, Artavanis Tsakonas S. The suppressor of hairless protein participates in notch receptor signaling. Cell. 1994;79:273-82 pubmed
    ..These results indicate that Notch activity may regulate nuclear events by controlling the activity of a DNA-binding protein. ..
  81. Turi T, Mueller U, Sazer S, Rose J. Characterization of a nuclear protein conferring brefeldin A resistance in Schizosaccharomyces pombe. J Biol Chem. 1996;271:9166-71 pubmed
    ..The protein encoded by hba1 is localized to the nucleus, and it was determined that this protein is phosphorylated in vivo. The characterization of hba1 thus demonstrates a novel mechanism of drug resistance in S. pombe. ..
  82. Finger F, Novick P. Spatial regulation of exocytosis: lessons from yeast. J Cell Biol. 1998;142:609-12 pubmed
  83. Sobel S, Wolin S. Two yeast La motif-containing proteins are RNA-binding proteins that associate with polyribosomes. Mol Biol Cell. 1999;10:3849-62 pubmed
    ..Thus, Sro9p and Slf1p define a new and possibly evolutionarily conserved class of La motif-containing proteins that may function in the cytoplasm to modulate mRNA translation. ..
  84. Barrett J, Manning B, Snyder M. The Kar3p kinesin-related protein forms a novel heterodimeric structure with its associated protein Cik1p. Mol Biol Cell. 2000;11:2373-85 pubmed
    ..These findings demonstrate that the Kar3-Cik1 complex has a novel heterodimeric structure not observed previously for kinesin complexes. ..
  85. Amerik A, Nowak J, Swaminathan S, Hochstrasser M. The Doa4 deubiquitinating enzyme is functionally linked to the vacuolar protein-sorting and endocytic pathways. Mol Biol Cell. 2000;11:3365-80 pubmed
  86. Wang W, Sacher M, Ferro Novick S. TRAPP stimulates guanine nucleotide exchange on Ypt1p. J Cell Biol. 2000;151:289-96 pubmed
    ..Furthermore, the purified TRAPP complex accelerates nucleotide exchange on Ypt1p. Our findings imply that Ypt1p, which is present on ER-to-Golgi transport vesicles, is activated at the Golgi once it interacts with TRAPP. ..
  87. Burchett S, Scott A, Errede B, Dohlman H. Identification of novel pheromone-response regulators through systematic overexpression of 120 protein kinases in yeast. J Biol Chem. 2001;276:26472-8 pubmed
    ..These results reveal two new components of the pheromone-signaling cascade in yeast, each acting at a point downstream of the MAPK. ..
  88. Du L, Novick P. Yeast rab GTPase-activating protein Gyp1p localizes to the Golgi apparatus and is a negative regulator of Ypt1p. Mol Biol Cell. 2001;12:1215-26 pubmed
    ..These results establish that Gyp1p functions on the Golgi as a negative regulator of Ypt1p. ..
  89. Long K, Cedervall T, Walch Solimena C, Noe D, Huddleston M, Annan R, et al. Phosphorylation of the Saccharomyces cerevisiae La protein does not appear to be required for its functions in tRNA maturation and nascent RNA stabilization. RNA. 2001;7:1589-602 pubmed
    ..Thus, although La proteins from yeast to humans are phosphoproteins, phosphorylation does not appear to be required for any of the identified functions of the S. cerevisiae protein. ..
  90. Bacon R, Salminen A, Ruohola H, Novick P, Ferro Novick S. The GTP-binding protein Ypt1 is required for transport in vitro: the Golgi apparatus is defective in ypt1 mutants. J Cell Biol. 1989;109:1015-22 pubmed
    ..We have also established genetic interactions between ypt1 and a subset of the other genes required for transport to and through the Golgi apparatus. ..
  91. Wang X, Kang D, Feng S, Serino G, Schwechheimer C, Wei N. CSN1 N-terminal-dependent activity is required for Arabidopsis development but not for Rub1/Nedd8 deconjugation of cullins: a structure-function study of CSN1 subunit of COP9 signalosome. Mol Biol Cell. 2002;13:646-55 pubmed
    ..We conclude that CSN1 N-terminal domain is not required for the Rub1/Nedd8 deconjugation activity of cullins, but contributes to a significant aspect of CSN functions that are essential for plant development. ..
  92. Tsubouchi H, Roeder G. The Mnd1 protein forms a complex with hop2 to promote homologous chromosome pairing and meiotic double-strand break repair. Mol Cell Biol. 2002;22:3078-88 pubmed
    ..The identification of Hop2 and Mnd1 homologs in other organisms suggests that the function of this complex is conserved among eukaryotes. ..