Genomes and Genes
Experts and Doctors on fluorescence microscopy in California, United States
Locale: California, United States
Topic: fluorescence microscopy
- Ishida T, Kundu R, Yang E, Hirata K, Ho Y, Quertermous T. Targeted disruption of endothelial cell-selective adhesion molecule inhibits angiogenic processes in vitro and in vivo. J Biol Chem. 2003;278:34598-604 pubmed..These in vitro and in vivo studies suggest that ESAM has a redundant functional role in physiological angiogenesis but serves a unique and essential role in pathological angiogenic processes such as tumor growth. ..
- Scheel J, Ray J, Gage F, Barlow C. Quantitative analysis of gene expression in living adult neural stem cells by gene trapping. Nat Methods. 2005;2:363-70 pubmed
- Prekeris R, Davies J, Scheller R. Identification of a novel Rab11/25 binding domain present in Eferin and Rip proteins. J Biol Chem. 2001;276:38966-70 pubmed..The data suggest that various Rab effectors compete with each other for binding to Rab11/25 possibly accounting for the diversity of Rab11 functions. ..
- Taupenot L, Harper K, O Connor D. Role of H+-ATPase-mediated acidification in sorting and release of the regulated secretory protein chromogranin A: evidence for a vesiculogenic function. J Biol Chem. 2005;280:3885-97 pubmed
- Maro G, Klassen M, Shen K. A beta-catenin-dependent Wnt pathway mediates anteroposterior axon guidance in C. elegans motor neurons. PLoS ONE. 2009;4:e4690 pubmed publisher..Together, our data provide evidence that Wnt-mediated axon guidance can be transduced through a beta-catenin-dependent pathway. ..
- Zhang Z, Smith B, Wang L, Brock A, Cho C, Schultz P. A new strategy for the site-specific modification of proteins in vivo. Biochemistry. 2003;42:6735-46 pubmed..The genetic incorporation of a nonproteinogenic "ketone handle" into proteins provides a powerful tool for the introduction of biophysical probes for the structural and functional analysis of proteins in vitro or in vivo. ..
- Leng T, Miller J, Bilbao K, Palanker D, Huie P, Blumenkranz M. The chick chorioallantoic membrane as a model tissue for surgical retinal research and simulation. Retina. 2004;24:427-34 pubmed..We describe the use of chick chorioallantoic membrane (CAM) as a model system for the study of the precision and safety of vitreoretinal microsurgical instruments and techniques...
- Rosell F, Boxer S. Polarized absorption spectra of green fluorescent protein single crystals: transition dipole moment directions. Biochemistry. 2003;42:177-83 pubmed
- Thompson M, Biteen J, Lord S, Conley N, Moerner W. Molecules and methods for super-resolution imaging. Methods Enzymol. 2010;475:27-59 pubmed publisher..Finally, a new method is described for obtaining three-dimensional super-resolution information using a double-helix point-spread function. ..
- Hostetter D, Rice S, Dean S, Altman D, McMahon P, Sutton S, et al. Dictyostelium myosin bipolar thick filament formation: importance of charge and specific domains of the myosin rod. PLoS Biol. 2004;2:e356 pubmed..Truncation analysis of AD-Cterm tail fragments shows that assembly is delicately balanced, resulting in assembled myosin-II molecules that are poised to disassemble due to the phosphorylation of only three threonines. ..
- Boyer S, Slesinger P, Jones S. Regulation of Kir2.1 channels by the Rho-GTPase, Rac1. J Cell Physiol. 2009;218:385-93 pubmed publisher..1 in mediating the potentiating effect of Rac1(DN). This novel pathway for regulating surface expression of cardiac Kir2.1 channels could have implications for normal and diseased cardiac states. ..
- Hintermann E, Yang N, O Sullivan D, Higgins J, Quaranta V. Integrin alpha6beta4-erbB2 complex inhibits haptotaxis by up-regulating E-cadherin cell-cell junctions in keratinocytes. J Biol Chem. 2005;280:8004-15 pubmed
- Beer S, Zetterberg A, Ihrie R, McTaggart R, Yang Q, Bradon N, et al. Developmental context determines latency of MYC-induced tumorigenesis. PLoS Biol. 2004;2:e332 pubmed..Adult somatic cells have evolved mechanisms to prevent individual oncogenes from initiating cellular growth, DNA replication, and mitotic cellular division alone, thereby preventing any single genetic event from inducing tumorigenesis. ..
- Hashimoto M, Sagara Y, Langford D, Everall I, Mallory M, Everson A, et al. Fibroblast growth factor 1 regulates signaling via the glycogen synthase kinase-3beta pathway. Implications for neuroprotection. J Biol Chem. 2002;277:32985-91 pubmed..Taken together these results suggest that neuroprotective effects of FGF1 might involve inactivation of GSK3beta by a pathway involving activation of the PI3K-Akt cascades. ..
- Tay S, Hughey J, Lee T, Lipniacki T, Quake S, Covert M. Single-cell NF-kappaB dynamics reveal digital activation and analogue information processing. Nature. 2010;466:267-71 pubmed publisher..These results highlight the value of high-throughput quantitative measurements with single-cell resolution in understanding how biological systems operate. ..
- Zhao C, Ma H, Bossy Wetzel E, Lipton S, Zhang Z, Feng G. GC-GAP, a Rho family GTPase-activating protein that interacts with signaling adapters Gab1 and Gab2. J Biol Chem. 2003;278:34641-53 pubmed
- Stenner Liewen F, Liewen H, Zapata J, Pawlowski K, Godzik A, Reed J. CADD, a Chlamydia protein that interacts with death receptors. J Biol Chem. 2002;277:9633-6 pubmed..trachomatis and co-localizes with Fas in the proximity of the inclusion body. The results suggest a role for CADD modulating the apoptosis pathways of cells infected, revealing a new mechanism of host-pathogen interaction. ..
- Koolpe M, Burgess R, Dail M, Pasquale E. EphB receptor-binding peptides identified by phage display enable design of an antagonist with ephrin-like affinity. J Biol Chem. 2005;280:17301-11 pubmed..These peptides should be useful as selective inhibitors of the pathological activities of EphB receptors and as targeting agents for imaging probes and therapeutic drugs. ..
- Levi J, Cheng Z, Gheysens O, Patel M, Chan C, Wang Y, et al. Fluorescent fructose derivatives for imaging breast cancer cells. Bioconjug Chem. 2007;18:628-34 pubmed..5 likely introduces big structural and electronic changes, leading to a fructose derivative that does not accurately describe the uptake of fructose in cells. ..
- McHale K, Mabuchi H. Precise characterization of the conformation fluctuations of freely diffusing DNA: beyond Rouse and Zimm. J Am Chem Soc. 2009;131:17901-7 pubmed publisher..Finally, we observe fluorescence fluctuations with a correlation time of over 2 s that cannot be explained by either model and propose that they may be signatures of excluded volume interactions. ..
- Savinov A, Rozanov D, Golubkov V, Wong F, Strongin A. Inhibition of membrane type-1 matrix metalloproteinase by cancer drugs interferes with the homing of diabetogenic T cells into the pancreas. J Biol Chem. 2005;280:27755-8 pubmed..Overall, our studies have divulged a previously unknown function of MT1-MMP and identified a promising novel drug target in type I diabetes. ..
- Brazelton T, Blau H. Optimizing techniques for tracking transplanted stem cells in vivo. Stem Cells. 2005;23:1251-65 pubmed
- Chan J, Taylor D, Zwerdling T, Lane S, Ihara K, Huser T. Micro-Raman spectroscopy detects individual neoplastic and normal hematopoietic cells. Biophys J. 2006;90:648-56 pubmed
- Plutner H, Davidson H, Saraste J, Balch W. Morphological analysis of protein transport from the ER to Golgi membranes in digitonin-permeabilized cells: role of the P58 containing compartment. J Cell Biol. 1992;119:1097-116 pubmed
- Song B, Yarar D, Schmid S. An assembly-incompetent mutant establishes a requirement for dynamin self-assembly in clathrin-mediated endocytosis in vivo. Mol Biol Cell. 2004;15:2243-52 pubmed..Together these results provide new structural insight into the role of GED in self-assembly and assembly-stimulated GTPase activity and establish that dynamin self-assembly is essential for clathrin-mediated endocytosis. ..
- Ju W, Liu Q, Kim K, Crowston J, Lindsey J, Agarwal N, et al. Elevated hydrostatic pressure triggers mitochondrial fission and decreases cellular ATP in differentiated RGC-5 cells. Invest Ophthalmol Vis Sci. 2007;48:2145-51 pubmed..Increased understanding of the molecular mechanisms that regulate the cellular response to elevated pressure including mitochondrial fission may provide new therapeutic targets for protecting RGCs from elevated hydrostatic pressure. ..
- Sharma M, Zarnegar M, Li X, Lim B, Sun Z. Androgen receptor interacts with a novel MYST protein, HBO1. J Biol Chem. 2000;275:35200-8 pubmed..These results indicate that HBO1 is a new AR-interacting protein capable of modulating AR activity. It could play a significant role in regulating AR-dependent genes in normal and prostate cancer cells. ..
- Rudge S, Anderson D, Emr S. Vacuole size control: regulation of PtdIns(3,5)P2 levels by the vacuole-associated Vac14-Fig4 complex, a PtdIns(3,5)P2-specific phosphatase. Mol Biol Cell. 2004;15:24-36 pubmed..Our studies indicate that Vac14 both positively regulates Fab1 kinase activity and directs the localization/activation of the Fig4 PtdIns(3,5)P2 phosphatase. ..
- Fryer C, Lamar E, Turbachova I, Kintner C, Jones K. Mastermind mediates chromatin-specific transcription and turnover of the Notch enhancer complex. Genes Dev. 2002;16:1397-411 pubmed..Thus MAM may function as a timer to couple transcription activation with disassembly of the Notch enhancer complex on chromatin. ..
- Martin L, Marth J, Varki A, Varki N. Genetically altered mice with different sialyltransferase deficiencies show tissue-specific alterations in sialylation and sialic acid 9-O-acetylation. J Biol Chem. 2002;277:32930-8 pubmed..These data demonstrate selective regulation of sialylation and 9-O-acetylation, point to cell types with potential physiological defects in null animals, and show in vivo evidence for competition between Golgi enzymes. ..
- Yeo M, Lin P, Dahmus M, Gill G. A novel RNA polymerase II C-terminal domain phosphatase that preferentially dephosphorylates serine 5. J Biol Chem. 2003;278:26078-85 pubmed..Accordingly, SCP1 may play a role in the regulation of gene expression, possibly by controlling the transition from initiation/capping to processive transcript elongation. ..
- Krisans S, Ericsson J, Edwards P, Keller G. Farnesyl-diphosphate synthase is localized in peroxisomes. J Biol Chem. 1994;269:14165-9 pubmed
- Kaido T, Perez B, Yebra M, Hill J, Cirulli V, Hayek A, et al. Alphav-integrin utilization in human beta-cell adhesion, spreading, and motility. J Biol Chem. 2004;279:17731-7 pubmed..By supporting fetal beta-cell migration, alpha(v)beta(1) could play an important role in early motile processes required for islet neogenesis. ..
- Gerber A, Herschlag D, Brown P. Extensive association of functionally and cytotopically related mRNAs with Puf family RNA-binding proteins in yeast. PLoS Biol. 2004;2:E79 pubmed
- De Belle I, Wu J, Sperandio S, Mercola D, Adamson E. In vivo cloning and characterization of a new growth suppressor protein TOE1 as a direct target gene of Egr1. J Biol Chem. 2003;278:14306-12 pubmed..Using this approach, we report the cloning of a new Egr1 target gene that is able to account, at least in part, for the growth inhibitory activity of Egr1. We have named this new protein TOE1 for target of Egr1. ..
- Balboa M, Shirai Y, Gaietta G, Ellisman M, Balsinde J, Dennis E. Localization of group V phospholipase A2 in caveolin-enriched granules in activated P388D1 macrophage-like cells. J Biol Chem. 2003;278:48059-65 pubmed
- Behrens S, Lösekann T, Pett Ridge J, Weber P, Ng W, Stevenson B, et al. Linking microbial phylogeny to metabolic activity at the single-cell level by using enhanced element labeling-catalyzed reporter deposition fluorescence in situ hybridization (EL-FISH) and NanoSIMS. Appl Environ Microbiol. 2008;74:3143-50 pubmed publisher..Our novel approach will facilitate further studies of the ecophysiology of known and uncultured microorganisms in complex environments and communities...
- Ratnikov B, Rozanov D, Postnova T, Baciu P, Zhang H, DiScipio R, et al. An alternative processing of integrin alpha(v) subunit in tumor cells by membrane type-1 matrix metalloproteinase. J Biol Chem. 2002;277:7377-85 pubmed
- Kim S, Hayashi M, Lo J, Yang Y, Yoo J, Lee J. ADP-ribosylation factor 4 small GTPase mediates epidermal growth factor receptor-dependent phospholipase D2 activation. J Biol Chem. 2003;278:2661-8 pubmed..Our findings indicate that ARF4 is a critical molecule that directly regulates cellular PLD2 activity and that this ARF4-mediated PLD2 activation stimulates AP-1-dependent transcription in the EGF-induced cellular response. ..
- Stanfield G, Villeneuve A. Regulation of sperm activation by SWM-1 is required for reproductive success of C. elegans males. Curr Biol. 2006;16:252-63 pubmed
- Deisseroth K, Feng G, Majewska A, Miesenbock G, Ting A, Schnitzer M. Next-generation optical technologies for illuminating genetically targeted brain circuits. J Neurosci. 2006;26:10380-6 pubmed..Here we explore key recent advances that unite optical and genetic approaches, focusing on promising techniques that either allow novel studies of neural dynamics and behavior or provide fresh perspectives on classic model systems. ..
- Fowler D, Koulov A, Alory Jost C, Marks M, Balch W, Kelly J. Functional amyloid formation within mammalian tissue. PLoS Biol. 2006;4:e6 pubmed
- Mitoma J, Bao X, Petryanik B, Schaerli P, Gauguet J, Yu S, et al. Critical functions of N-glycans in L-selectin-mediated lymphocyte homing and recruitment. Nat Immunol. 2007;8:409-18 pubmed..Our results demonstrate the critical function of N-glycan-linked 6-sulfo sialyl Lewis X in L-selectin-dependent lymphocyte homing and recruitment. ..
- Li S, Zeng L, Feng L, Chen D. Rac1-dependent intracellular superoxide formation mediates vascular endothelial growth factor-induced placental angiogenesis in vitro. Endocrinology. 2010;151:5315-25 pubmed publisher..This NADPH oxidase system appears to generate the second messenger superoxide that plays a critical role in the signaling control of the VEGF-induced placental angiogenesis. ..
- Mas P, Devlin P, Panda S, Kay S. Functional interaction of phytochrome B and cryptochrome 2. Nature. 2000;408:207-11 pubmed..Using fluorescent resonance energy transfer microscopy, we show that phyB and cry2 interact in nuclear speckles that are formed in a light-dependent fashion. ..
- Stallcup W, Dahlin K, Healy P. Interaction of the NG2 chondroitin sulfate proteoglycan with type VI collagen. J Cell Biol. 1990;111:3177-88 pubmed..Our results suggest that cell surface NG2 may mediate cellular interactions with the extracellular matrix by binding to type VI collagen. ..
- Zhang Y, Luo W, Wang H, Lin P, Vetrivel K, Liao F, et al. Nicastrin is critical for stability and trafficking but not association of other presenilin/gamma-secretase components. J Biol Chem. 2005;280:17020-6 pubmed
- Smythe G, Eby J, Disatnik M, Rando T. A caveolin-3 mutant that causes limb girdle muscular dystrophy type 1C disrupts Src localization and activity and induces apoptosis in skeletal myotubes. J Cell Sci. 2003;116:4739-49 pubmed
- Rousset R, Mack J, Wharton K, Axelrod J, Cadigan K, Fish M, et al. Naked cuticle targets dishevelled to antagonize Wnt signal transduction. Genes Dev. 2001;15:658-71 pubmed..Our results suggest that Nkd acts directly through Dsh to limit Wg activity and thus determines how efficiently Wnt signals stabilize Armadillo (Arm)/beta-catenin and activate downstream genes. ..
- Wu S, Xie P, Welsh K, Li C, Ni C, Zhu X, et al. LMP1 protein from the Epstein-Barr virus is a structural CD40 decoy in B lymphocytes for binding to TRAF3. J Biol Chem. 2005;280:33620-6 pubmed
- Mulholland J, Preuss D, Moon A, Wong A, Drubin D, Botstein D. Ultrastructure of the yeast actin cytoskeleton and its association with the plasma membrane. J Cell Biol. 1994;125:381-91 pubmed..We propose a possible role for this unique cortical structure in wall growth and osmotic regulation. ..
- Saito A, Hayashi T, Okuno S, Nishi T, Chan P. Modulation of proline-rich akt substrate survival signaling pathways by oxidative stress in mouse brains after transient focal cerebral ischemia. Stroke. 2006;37:513-7 pubmed..These results suggest that overexpression of SOD1 may affect the PRAS pathway after tFCI by reducing the direct oxidative reaction to pPRAS after reperfusion injury. ..
- Guan T, Kehlenbach R, Schirmer E, Kehlenbach A, Fan F, Clurman B, et al. Nup50, a nucleoplasmically oriented nucleoporin with a role in nuclear protein export. Mol Cell Biol. 2000;20:5619-30 pubmed..Taken together, our data indicate that Nup50 has a direct role in nuclear protein export and probably serves as a binding site on the nuclear side of the pore complex for export receptor-cargo complexes. ..
- Shibagaki N, Grossman A. Binding of cysteine synthase to the STAS domain of sulfate transporter and its regulatory consequences. J Biol Chem. 2010;285:25094-102 pubmed publisher..These observations suggest a regulatory model in which interactions between SULTR1;2 and OASTL coordinate internalization of SO(4)(2-) with the energetic/metabolic state of plant root cells. ..
- Yachi P, Ampudia J, Gascoigne N, Zal T. Nonstimulatory peptides contribute to antigen-induced CD8-T cell receptor interaction at the immunological synapse. Nat Immunol. 2005;6:785-92 pubmed..Thus, the interaction of CD8 with a nonstimulatory peptide-MHC complex helps mediate T cell recognition of antigen, improving the coreceptor function of CD8. ..
- Chen M, Tao Y, Lim J, Shaw A, Chory J. Regulation of phytochrome B nuclear localization through light-dependent unmasking of nuclear-localization signals. Curr Biol. 2005;15:637-42 pubmed
- Denis V, Cyert M. Molecular analysis reveals localization of Saccharomyces cerevisiae protein kinase C to sites of polarized growth and Pkc1p targeting to the nucleus and mitotic spindle. Eukaryot Cell. 2005;4:36-45 pubmed..This novel nuclear and spindle localization of Pkc1p may provide a molecular explanation for previous observations that suggest a role for Pkc1p in regulating microtubule function. ..
- Pilch J, Habermann R, Felding Habermann B. Unique ability of integrin alpha(v)beta 3 to support tumor cell arrest under dynamic flow conditions. J Biol Chem. 2002;277:21930-8 pubmed..We conclude that the presence of alpha(v)beta3 in a functionally activated state provides a unique advantage for circulating tumor cells by promoting tumor cell arrest in the presence of flow-dependent shear forces. ..
- Fagegaltier D, Baker B. X chromosome sites autonomously recruit the dosage compensation complex in Drosophila males. PLoS Biol. 2004;2:e341 pubmed..We propose a new model in which the distribution of compensasomes along the X chromosome is achieved according to the hierarchical affinities of individual binding sites. ..
- Aoka Y, Johnson F, Penta K, Hirata Ki K, Hidai C, Schatzman R, et al. The embryonic angiogenic factor Del1 accelerates tumor growth by enhancing vascular formation. Microvasc Res. 2002;64:148-61 pubmed..Taken together, these data suggest that Del1 acts as an angiogenic factor in the context of solid tumor formation and that this increase in vascularization accelerates tumor growth through decreased apoptosis. ..
- Alonso A, Narisawa S, Bogetz J, Tautz L, Hadzic R, Huynh H, et al. VHY, a novel myristoylated testis-restricted dual specificity protein phosphatase related to VHX. J Biol Chem. 2004;279:32586-91 pubmed..Finally, we demonstrate that VHY is an active phosphatase in vitro. We conclude that VHY is a new member of a subgroup of myristoylated VH1-like small dual specificity phosphatases. ..
- Rosario H, Waldo S, Becker S, Schmid Schonbein G. Pancreatic trypsin increases matrix metalloproteinase-9 accumulation and activation during acute intestinal ischemia-reperfusion in the rat. Am J Pathol. 2004;164:1707-16 pubmed..This process represents a powerful in vivo pathophysiological mechanism for trypsin-induced MMP-9 activation and is likely to play a central role in the development of acute intestinal inflammation and shock. ..
- Kunkel E, Kim C, Lazarus N, Vierra M, Soler D, Bowman E, et al. CCR10 expression is a common feature of circulating and mucosal epithelial tissue IgA Ab-secreting cells. J Clin Invest. 2003;111:1001-10 pubmed
- Lee H, Dunzendorfer S, Tobias P. Cytoplasmic domain-mediated dimerizations of toll-like receptor 4 observed by beta-lactamase enzyme fragment complementation. J Biol Chem. 2004;279:10564-74 pubmed..These findings broaden our understanding of TLR self-interactions as well as heterodimer formation. ..
- Zhang Y, Frangos J, Chachisvilis M. Mechanical stimulus alters conformation of type 1 parathyroid hormone receptor in bone cells. Am J Physiol Cell Physiol. 2009;296:C1391-9 pubmed publisher..We therefore suggest that PTH1R is involved in mediating primary mechanochemical signal transduction in MC3T3-E1 cells. ..
- Corriden R, Chen Y, Inoue Y, Beldi G, Robson S, Insel P, et al. Ecto-nucleoside triphosphate diphosphohydrolase 1 (E-NTPDase1/CD39) regulates neutrophil chemotaxis by hydrolyzing released ATP to adenosine. J Biol Chem. 2008;283:28480-6 pubmed publisher..Thus, E-NTPDase1 plays an important role in regulating neutrophil chemotaxis by facilitating the hydrolysis of extracellular ATP. ..
- Sundd P, Gutierrez E, Pospieszalska M, Zhang H, Groisman A, Ley K. Quantitative dynamic footprinting microscopy reveals mechanisms of neutrophil rolling. Nat Methods. 2010;7:821-4 pubmed publisher..Each rolling neutrophil formed three to four long tethers that extended up to 16 ?m behind the rolling cell. ..
- Van Wye J, Ghori N, Webster P, Mitschler R, Elmendorf H, Haldar K. Identification and localization of rab6, separation of rab6 from ERD2 and implications for an 'unstacked' Golgi, in Plasmodium falciparum. Mol Biochem Parasitol. 1996;83:107-20 pubmed..Hence the Golgi of ring stage parasites may comprise of multiple, 'unstacked' tubulovesicular clusters, suggesting a primitive organization of the organelle in Plasmodia. ..
- Manabe S, Gu Z, Lipton S. Activation of matrix metalloproteinase-9 via neuronal nitric oxide synthase contributes to NMDA-induced retinal ganglion cell death. Invest Ophthalmol Vis Sci. 2005;46:4747-53 pubmed..In addition, the broad-spectrum MMP inhibitor GM6001 protected RGCs after intravitreal NMDA injection. These findings suggest that an extracellular proteolytic pathway in the retina contributes to RGC death via NO-activated MMP-9. ..
- Wang W, Ding J, Allen E, Zhu P, Zhang L, Vogel H, et al. Gigaxonin interacts with tubulin folding cofactor B and controls its degradation through the ubiquitin-proteasome pathway. Curr Biol. 2005;15:2050-5 pubmed..Our study provides important insight into pathogenesis of neurodegenerative diseases associated with cytoskeletal abnormalities. ..
- Ponti A, Matov A, Adams M, Gupton S, Waterman Storer C, Danuser G. Periodic patterns of actin turnover in lamellipodia and lamellae of migrating epithelial cells analyzed by quantitative Fluorescent Speckle Microscopy. Biophys J. 2005;89:3456-69 pubmed..Several algorithmic advances in speckle image processing are described enabling the analysis of kinetic and kinematic activities of polymer networks at the level of single speckles...
- Golubkov V, Boyd S, Savinov A, Chekanov A, Osterman A, Remacle A, et al. Membrane type-1 matrix metalloproteinase (MT1-MMP) exhibits an important intracellular cleavage function and causes chromosome instability. J Biol Chem. 2005;280:25079-86 pubmed..We believe that our data show a novel proteolytic pathway to chromatin instability and elucidate the close association of MT1-MMP with malignant transformation. ..
- Axelrod J. Unipolar membrane association of Dishevelled mediates Frizzled planar cell polarity signaling. Genes Dev. 2001;15:1182-7 pubmed..This transition from symmetric to asymmetric Dsh localization requires Dsh function, and reflects an amplification process that generates a steep intracellular activity gradient necessary to determine PCP. ..
- Du L, Moser B, Russell P. Homo-oligomerization is the essential function of the tandem BRCT domains in the checkpoint protein Crb2. J Biol Chem. 2004;279:38409-14 pubmed..These results demonstrate that the crucial function of the tandem BRCT domains is to oligomerize Crb2. ..
- Degryse B, Neels J, Czekay R, Aertgeerts K, Kamikubo Y, Loskutoff D. The low density lipoprotein receptor-related protein is a motogenic receptor for plasminogen activator inhibitor-1. J Biol Chem. 2004;279:22595-604 pubmed..Activation of this LRP-dependent signaling pathway by PAI-1 may begin to explain how the inhibitor stimulates cell migration in a variety of normal and pathological processes. ..
- Vervoort V, Roselli S, Oshima R, Pasquale E. Splice variants and expression patterns of SHEP1, BCAR3 and NSP1, a gene family involved in integrin and receptor tyrosine kinase signaling. Gene. 2007;391:161-70 pubmed..These expression patterns suggest differential roles for the three genes during breast cancer progression in either the vasculature or the tumor cells. ..
- Feng W, Yasumura D, Matthes M, Lavail M, Vollrath D. Mertk triggers uptake of photoreceptor outer segments during phagocytosis by cultured retinal pigment epithelial cells. J Biol Chem. 2002;277:17016-22 pubmed..We conclude that Mertk likely functions directly in the RPE phagocytic process as a signaling molecule triggering outer segment ingestion. ..
- Coulon S, Gaillard P, Chahwan C, McDonald W, Yates J, Russell P. Slx1-Slx4 are subunits of a structure-specific endonuclease that maintains ribosomal DNA in fission yeast. Mol Biol Cell. 2004;15:71-80 pubmed..pombe. We propose a model in which the Slx1-Slx4 complex is involved in the control of the expansion and contraction of the rDNA loci by initiating recombination events at stalled RFs. ..
- Aschenbrenner L, Lee T, Hasson T. Myo6 facilitates the translocation of endocytic vesicles from cell peripheries. Mol Biol Cell. 2003;14:2728-43 pubmed
- Gao H, Chen X, McGowan C. Mus81 endonuclease localizes to nucleoli and to regions of DNA damage in human S-phase cells. Mol Biol Cell. 2003;14:4826-34 pubmed..Together these data support a role for Mus81 in recombination repair in higher eukaryotes. ..
- Cadigan K, Nusse R. wingless signaling in the Drosophila eye and embryonic epidermis. Development. 1996;122:2801-12 pubmed..However, we present evidence that wingless signaling still occurs normally in the complete absence of Notch protein in the embryonic epidermis. Thus, in the simplest model for wingless signalling, a direct role for Notch is unlikely. ..
- Denault J, Salvesen G. Human caspase-7 activity and regulation by its N-terminal peptide. J Biol Chem. 2003;278:34042-50 pubmed..The N-peptide must first be removed, probably by caspase-3, before efficient conversion and activation of the zymogen can occur in vivo. ..
- Lillemeier B, Mörtelmaier M, Forstner M, Huppa J, Groves J, Davis M. TCR and Lat are expressed on separate protein islands on T cell membranes and concatenate during activation. Nat Immunol. 2010;11:90-6 pubmed publisher..This separation versus physical juxtapositioning of receptor domains and domains containing downstream signaling molecules in quiescent versus activated T cells may be a general feature of plasma membrane-associated signal transduction. ..
- Robison B, Reisenbichler K, Hunt J, Haddock S. Light production by the arm tips of the deep-sea cephalopod Vampyroteuthis infernalis. Biol Bull. 2003;205:102-9 pubmed..This paper presents observations on the structure and operation of the arm-tip light organs, the character of the luminous cloud, and how the light they produce is incorporated into behavioral patterns. ..
- Sullivan B, Karpen G. Centromeric chromatin exhibits a histone modification pattern that is distinct from both euchromatin and heterochromatin. Nat Struct Mol Biol. 2004;11:1076-83 pubmed..We speculate that this distinct modification pattern contributes to the unique domain organization and three-dimensional structure of centromeric regions, and/or to the epigenetic information that determines centromere identity. ..
- Hayashi M, Imanaka Yoshida K, Yoshida T, Wood M, Fearns C, Tatake R, et al. A crucial role of mitochondrial Hsp40 in preventing dilated cardiomyopathy. Nat Med. 2006;12:128-32 pubmed..These findings suggest that Dnaja3 is crucial for mitochondrial biogenesis, at least in part, through its chaperone activity on Polga and provide genetic evidence of the necessity for mitochondrial Hsp40 in preventing DCM. ..
- Chan J, Hamilton C, Cheung M, Karimi M, Baker J, Gall J, et al. Enhanced killing of primary ovarian cancer by retargeting autologous cytokine-induced killer cells with bispecific antibodies: a preclinical study. Clin Cancer Res. 2006;12:1859-67 pubmed..Bispecific antibodies significantly enhanced the cytotoxicity of CIK cells in primary ovarian cancer cells and in our in vivo mouse model. The mechanism of cytolysis seems to be mediated in part by the NKG2D receptor. ..