Genomes and Genes
Experts and Doctors on dna binding proteins in Scotland, United Kingdom
Locale: Scotland, United Kingdom
Topic: dna binding proteins
- Precious B, Carlos T, Goodbourn S, Randall R. Catalytic turnover of STAT1 allows PIV5 to dismantle the interferon-induced anti-viral state of cells. Virology. 2007;368:114-21 pubmed..The importance of this mechanism in terms of the ability of the virus to dismantle the IFN-induced anti-viral state of cells is discussed. ..
- Bonnesen C, Eggleston I, Hayes J. Dietary indoles and isothiocyanates that are generated from cruciferous vegetables can both stimulate apoptosis and confer protection against DNA damage in human colon cell lines. Cancer Res. 2001;61:6120-30 pubmed..Similar results were obtained with H(2)O(2) as the genotoxic agent. Together, these phytochemicals may prevent colon tumorigenesis by both stimulating apoptosis and enhancing intracellular defenses against genotoxic agents. ..
- Nioi P, McMahon M, Itoh K, Yamamoto M, Hayes J. Identification of a novel Nrf2-regulated antioxidant response element (ARE) in the mouse NAD(P)H:quinone oxidoreductase 1 gene: reassessment of the ARE consensus sequence. Biochem J. 2003;374:337-48 pubmed..Furthermore, our results indicate that distinct AREs have differential sequence requirements, and a universally applicable consensus sequence cannot be derived. ..
- Boner W, Taylor E, Tsirimonaki E, Yamane K, Campo M, Morgan I. A Functional interaction between the human papillomavirus 16 transcription/replication factor E2 and the DNA damage response protein TopBP1. J Biol Chem. 2002;277:22297-303 pubmed..Removal of the amino-terminal domain of TopBP1 abolishes coactivation of transcription and replication. This interaction may have functional consequences upon the viral life cycle. ..
- Hall Jackson C, Cross D, Morrice N, Smythe C. ATR is a caffeine-sensitive, DNA-activated protein kinase with a substrate specificity distinct from DNA-PK. Oncogene. 1999;18:6707-13 pubmed..Our results are consistent with the notion that ATR plays a role in monitoring DNA structure and phosphorylation of proteins involved in the DNA damage response pathways. ..
- Morrison L, Pierotti A. Thimet oligopeptidase expression is differentially regulated in neuroendocrine and spermatid cell lines by transcription factor binding to SRY (sex-determining region Y), CAAT and CREB (cAMP-response-element-binding protein) promoter consensus sequen. Biochem J. 2003;376:189-97 pubmed..15 expression is regulated by SRY and CREB/CREM (cAMP-response element modulator), the principle testes-determining protein and the major orchestrator of spermatogenesis respectively. ..
- Leslie A, Pratt N, Gillespie K, Sales M, Kernohan N, Smith G, et al. Mutations of APC, K-ras, and p53 are associated with specific chromosomal aberrations in colorectal adenocarcinomas. Cancer Res. 2003;63:4656-61 pubmed..011). The associations we have described should refine the search for candidate genes underlying chromosomal aberrations and assist in the definition of distinct pathways in colorectal tumorigenesis. ..
- Jimenez L, Thompson J, Brown D, Rahman I, Antonicelli F, Duffin R, et al. Activation of NF-kappaB by PM(10) occurs via an iron-mediated mechanism in the absence of IkappaB degradation. Toxicol Appl Pharmacol. 2000;166:101-10 pubmed..Coincubation of polymixin B, an endotoxin-binding compound, and PM(10) did not inhibit NF-kappaB. In summary, PM(10) activates NF-kappaB in A549 cells by an iron-mediated mechanism in the absence of IkappaB degradation. ..
- Hay D, Sutherland L, Clark J, Burdon T. Oct-4 knockdown induces similar patterns of endoderm and trophoblast differentiation markers in human and mouse embryonic stem cells. Stem Cells. 2004;22:225-35 pubmed..Moreover, the upregulation of endoderm-associated markers in both mouse and human ES cells points to overlap between development of trophoblast and endoderm differentiation. ..
- Goodridge H, Marshall F, Else K, Houston K, Egan C, Al Riyami L, et al. Immunomodulation via novel use of TLR4 by the filarial nematode phosphorylcholine-containing secreted product, ES-62. J Immunol. 2005;174:284-93 pubmed
- Woodward A, Gohler T, Luciani M, Oehlmann M, Ge X, Gartner A, et al. Excess Mcm2-7 license dormant origins of replication that can be used under conditions of replicative stress. J Cell Biol. 2006;173:673-83 pubmed
- Alzuherri H, Chang K. Calcineurin activates NF-kappaB in skeletal muscle C2C12 cells. Cell Signal. 2003;15:471-8 pubmed..We therefore showed a functional interaction between the CnA and NF-kappaB pathways in skeletal muscle cells, which involved opposing phenotypic effects of CnA. ..
- Darragh J, Soloaga A, Beardmore V, Wingate A, Wiggin G, Peggie M, et al. MSKs are required for the transcription of the nuclear orphan receptors Nur77, Nurr1 and Nor1 downstream of MAPK signalling. Biochem J. 2005;390:749-59 pubmed..Downstream of anisomycin signalling, a second ERK-dependent pathway, independent of MSK and CREB, was also required for the transcription of Nurr1 and Nor1. ..
- Auld G, Campbell D, Morrice N, Cohen P. Identification of calcium-regulated heat-stable protein of 24 kDa (CRHSP24) as a physiological substrate for PKB and RSK using KESTREL. Biochem J. 2005;389:775-83 pubmed
- Vitalis T, Cases O, Engelkamp D, Verney C, Price D. Defect of tyrosine hydroxylase-immunoreactive neurons in the brains of mice lacking the transcription factor Pax6. J Neurosci. 2000;20:6501-16 pubmed..This may be responsible for both the altered pathway of catecholaminergic fibers and the ectopic location of catecholaminergic neurons in this region. ..
- Devling T, Lindsay C, McLellan L, McMahon M, Hayes J. Utility of siRNA against Keap1 as a strategy to stimulate a cancer chemopreventive phenotype. Proc Natl Acad Sci U S A. 2005;102:7280-7285A pubmed..75-fold increase in intracellular glutathione 48 h after transfection. Thus, antagonism of Keap1 by siRNA can be used to preadapt human cells to oxidative stress without the need to expose them to redox stressors. ..
- McMahon M, Itoh K, Yamamoto M, Chanas S, Henderson C, McLellan L, et al. The Cap'n'Collar basic leucine zipper transcription factor Nrf2 (NF-E2 p45-related factor 2) controls both constitutive and inducible expression of intestinal detoxification and glutathione biosynthetic enzymes. Cancer Res. 2001;61:3299-307 pubmed
- Feng X, Colloms S. In vitro transposition of ISY100, a bacterial insertion sequence belonging to the Tc1/mariner family. Mol Microbiol. 2007;65:1432-43 pubmed..Our results demonstrate that there are no fundamental differences between the transposition mechanisms of IS630 family elements in bacteria and Tc1/mariner elements in higher eukaryotes. ..
- Chanas S, Jiang Q, McMahon M, McWalter G, McLellan L, Elcombe C, et al. Loss of the Nrf2 transcription factor causes a marked reduction in constitutive and inducible expression of the glutathione S-transferase Gsta1, Gsta2, Gstm1, Gstm2, Gstm3 and Gstm4 genes in the livers of male and female mice. Biochem J. 2002;365:405-16 pubmed..These data also indicate that attenuated induction of GSH-dependent enzymes in Nrf2(-/-) mice probably accounts for their failure to adapt to chronic exposure to chemical and oxidative stress. ..
- McWalter G, Higgins L, McLellan L, Henderson C, Song L, Thornalley P, et al. Transcription factor Nrf2 is essential for induction of NAD(P)H:quinone oxidoreductase 1, glutathione S-transferases, and glutamate cysteine ligase by broccoli seeds and isothiocyanates. J Nutr. 2004;134:3499S-3506S pubmed publisher..These experiments show that broccoli seeds are effective at inducing antioxidant and detoxication proteins, both in vivo and ex vivo, in an Nrf2-dependent manner. ..
- McMahon M, Thomas N, Itoh K, Yamamoto M, Hayes J. Redox-regulated turnover of Nrf2 is determined by at least two separate protein domains, the redox-sensitive Neh2 degron and the redox-insensitive Neh6 degron. J Biol Chem. 2004;279:31556-67 pubmed..A model can now be proposed to explain how the turnover of this protein adapts in response to alterations in cellular redox state. ..
- Chambers I, Colby D, Robertson M, Nichols J, Lee S, Tweedie S, et al. Functional expression cloning of Nanog, a pluripotency sustaining factor in embryonic stem cells. Cell. 2003;113:643-55 pubmed..These findings establish a central role for Nanog in the transcription factor hierarchy that defines ES cell identity. ..
- Haddad J. Redox regulation of pro-inflammatory cytokines and IkappaB-alpha/NF-kappaB nuclear translocation and activation. Biochem Biophys Res Commun. 2002;296:847-56 pubmed
- Ying Q, Nichols J, Chambers I, Smith A. BMP induction of Id proteins suppresses differentiation and sustains embryonic stem cell self-renewal in collaboration with STAT3. Cell. 2003;115:281-92 pubmed..Upon LIF withdrawal, Id-expressing ES cells differentiate but do not give rise to neural lineages. We conclude that blockade of lineage-specific transcription factors by Id proteins enables the self-renewal response to LIF/STAT3. ..
- Robertson M, Stenhouse F, Colby D, Marland J, Nichols J, Tweedie S, et al. Nanog retrotransposed genes with functionally conserved open reading frames. Mamm Genome. 2006;17:732-43 pubmed..caroli and M. spretus and indicates that Nanog retrotransposition events continue to occur at a high frequency, a property likely to extend to other germ-line transcripts. ..
- Messager S, Hazlerigg D, Mercer J, Morgan P. Photoperiod differentially regulates the expression of Per1 and ICER in the pars tuberalis and the suprachiasmatic nucleus of the Siberian hamster. Eur J Neurosci. 2000;12:2865-70 pubmed..These data demonstrate that photoperiod-dependent amplitude modulation of Per1 and ICER gene expression in the PT is conserved across species, and reinforce the argument that this phenomenon is driven by melatonin. ..
- Rena G, Guo S, Cichy S, Unterman T, Cohen P. Phosphorylation of the transcription factor forkhead family member FKHR by protein kinase B. J Biol Chem. 1999;274:17179-83 pubmed..The conservation of Thr-24, Ser-256, and Ser-319 and the sequences surrounding them in DAF16 therefore suggests that DAF16 is also a direct substrate for PKB in C. elegans. ..
- Richards J, Johnson K, Liu H, McRobbie A, McMahon S, Oke M, et al. Structure of the DNA repair helicase hel308 reveals DNA binding and autoinhibitory domains. J Biol Chem. 2008;283:5118-26 pubmed publisher..These data suggest that one function of the enzyme may be in the removal of bound proteins at stalled replication forks and recombination intermediates...
- Kongkanuntn R, Bubb V, Sansom O, Wyllie A, Harrison D, Clarke A. Dysregulated expression of beta-catenin marks early neoplastic change in Apc mutant mice, but not all lesions arising in Msh2 deficient mice. Oncogene. 1999;18:7219-25 pubmed
- Prost S, Bellamy C, Cunningham D, Harrison D. Altered DNA repair and dysregulation of p53 in IRF-1 null hepatocytes. FASEB J. 1998;12:181-8 pubmed..Hence, in hepatocytes, IRF-1 is not required for growth arrest or apoptosis after DNA damage, but the results suggest for the first time a role in DNA repair regulation. ..
- Klose R, Bird A. MeCP2 behaves as an elongated monomer that does not stably associate with the Sin3a chromatin remodeling complex. J Biol Chem. 2004;279:46490-6 pubmed..Our findings indicate the MeCP2 is not an obligate component of the Sin3a corepressor complex and may therefore engage a more diverse range of cofactors for repressive function. ..
- Chambers I, Smith A. Self-renewal of teratocarcinoma and embryonic stem cells. Oncogene. 2004;23:7150-60 pubmed..Nanog function also requires Oct4. Here, we review recent progress in ES cell self-renewal, relate this to the biology of teratocarcinomas and offer testable hypotheses to expose the mechanics of ES cell self-renewal...
- Lancaster O, Cullen C, Ohkura H. NHK-1 phosphorylates BAF to allow karyosome formation in the Drosophila oocyte nucleus. J Cell Biol. 2007;179:817-24 pubmed..We propose that BAF phosphorylation by NHK-1 disrupts anchorage of chromosomes to the nuclear envelope, allowing karyosome formation in oocytes. These data provide the first mechanistic insight into how the karyosome forms. ..
- Duro E, Lundin C, Ask K, Sanchez Pulido L, MacArtney T, Toth R, et al. Identification of the MMS22L-TONSL complex that promotes homologous recombination. Mol Cell. 2010;40:632-44 pubmed publisher..Therefore, MMS22L and TONSL are required for the maintenance of genome stability when unscheduled DSBs occur in the vicinity of DNA replication forks. ..
- Aubert J, Stavridis M, Tweedie S, O Reilly M, Vierlinger K, Li M, et al. Screening for mammalian neural genes via fluorescence-activated cell sorter purification of neural precursors from Sox1-gfp knock-in mice. Proc Natl Acad Sci U S A. 2003;100 Suppl 1:11836-41 pubmed..Whole mount in situ hybridization against two genes of previously unknown embryonic expression, Lrrn1 and Musashi2, confirmed the selectivity of this screen for early neuroectodermal markers. ..
- Gillespie P, Khoudoli G, Stewart G, Swedlow J, Blow J. ELYS/MEL-28 chromatin association coordinates nuclear pore complex assembly and replication licensing. Curr Biol. 2007;17:1657-62 pubmed..Because nuclear assembly is required to shut down licensing prior to entry into S phase, our results suggest a mechanism by which these two early cell-cycle events are coordinated with one another. ..
- Hay D, Kemp G, Dargemont C, Hay R. Interaction between hnRNPA1 and IkappaBalpha is required for maximal activation of NF-kappaB-dependent transcription. Mol Cell Biol. 2001;21:3482-90 pubmed..Thus in addition to regulating mRNA processing and transport, hnRNPA1 also contributes to the control of NF-kappaB-dependent transcription. ..
- Hayes J, Chanas S, Henderson C, McMahon M, Sun C, Moffat G, et al. The Nrf2 transcription factor contributes both to the basal expression of glutathione S-transferases in mouse liver and to their induction by the chemopreventive synthetic antioxidants, butylated hydroxyanisole and ethoxyquin. Biochem Soc Trans. 2000;28:33-41 pubmed..Significantly, residual induction of GST by antioxidants is apparent in the Nrf2 mutant mouse, indicating the existence of an alternative mechanism of gene activation. ..
- Zhang C, Clarke P. Chromatin-independent nuclear envelope assembly induced by Ran GTPase in Xenopus egg extracts. Science. 2000;288:1429-32 pubmed..Thus, concentration of Ran-GDP followed by generation of Ran-GTP is sufficient to induce NE assembly. ..
- Dormer U, Westwater J, McLaren N, Kent N, Mellor J, Jamieson D. Cadmium-inducible expression of the yeast GSH1 gene requires a functional sulfur-amino acid regulatory network. J Biol Chem. 2000;275:32611-6 pubmed..In particular the transcription factors Met-4, Met-31, and Met-32 are essential for cadmium-mediated regulation of gene expression, whereas the DNA-binding protein Cbf1 appears to play a negative role in controlling GSH1 expression. ..
- McCool S, Pierotti A. Expression of the thimet oligopeptidase gene is regulated by positively and negatively acting elements. DNA Cell Biol. 2000;19:729-38 pubmed..This work has identified a number of transcription factor-binding sites. However, no differences in the binding behavior in the various cell lines was observed. ..
- Nilaweera K, Ellis C, Barrett P, Mercer J, Morgan P. Hypothalamic bHLH transcription factors are novel candidates in the regulation of energy balance. Eur J Neurosci. 2002;15:644-50 pubmed..Nhlh-2 mRNA was coexpressed in a subpopulation (30%) of ARC neurons expressing pro-opiomelanocortin (POMC) mRNA, suggesting a potential functional relationship. ..
- Dani C, Chambers I, Johnstone S, Robertson M, Ebrahimi B, Saito M, et al. Paracrine induction of stem cell renewal by LIF-deficient cells: a new ES cell regulatory pathway. Dev Biol. 1998;203:149-62 pubmed..Operation of this pathway in vivo could play an important role in the regulation of pluripotency in the epiblast and account for the viability of lifr -/- and gp130 -/- embryos. ..
- Lewis J, Meehan R, Henzel W, Maurer Fogy I, Jeppesen P, Klein F, et al. Purification, sequence, and cellular localization of a novel chromosomal protein that binds to methylated DNA. Cell. 1992;69:905-14 pubmed..In mouse, for example, MeCP2 is concentrated in pericentromeric heterochromatin, which contains a large fraction (about 40%) of all genomic 5-methylcytosine. ..
- Horvat S, Medrano J. Lack of Socs2 expression causes the high-growth phenotype in mice. Genomics. 2001;72:209-12 pubmed..These results demonstrate a critical role for Socs2 in controlling growth. ..
- Paterson J, Smith S, Harmar A, Antoni F. Control of a novel adenylyl cyclase by calcineurin. Biochem Biophys Res Commun. 1995;214:1000-8 pubmed..The enzyme messenger RNA is particularly enriched in the cerebral cortex, striatum and hippocampus, where it is localized to neuronal perikarya, indicative of an important role in neuronal function. ..
- Munoz I, Jowsey P, Toth R, Rouse J. Phospho-epitope binding by the BRCT domains of hPTIP controls multiple aspects of the cellular response to DNA damage. Nucleic Acids Res. 2007;35:5312-22 pubmed..Thus, the BRCT domains of hPTIP play important roles in the cellular response to DNA damage. ..
- Kaji K, Caballero I, MacLeod R, Nichols J, Wilson V, Hendrich B. The NuRD component Mbd3 is required for pluripotency of embryonic stem cells. Nat Cell Biol. 2006;8:285-92 pubmed..Our findings define a role for epigenetic silencing in the cell-fate commitment of pluripotent cells. ..
- Naz N, Young H, Ahmed N, Gadd G. Cadmium accumulation and DNA homology with metal resistance genes in sulfate-reducing bacteria. Appl Environ Microbiol. 2005;71:4610-8 pubmed
- Hansen S, Lane D, Midgley C. The N terminus of the murine p53 tumour suppressor is an independent regulatory domain affecting activation and thermostability. J Mol Biol. 1998;275:575-88 pubmed
- Engelkamp D, Rashbass P, Seawright A, van Heyningen V. Role of Pax6 in development of the cerebellar system. Development. 1999;126:3585-96 pubmed..Our results suggest that Pax6 plays a strong role during hindbrain migration processes and at least part of its activity is mediated through regulation of the netrin receptor Unc5h3. ..
- Okoye A, Cordano P, Taylor E, Morgan I, Everett R, Campo M. Human papillomavirus 16 L2 inhibits the transcriptional activation function, but not the DNA replication function, of HPV-16 E2. Virus Res. 2005;108:1-14 pubmed..The consequences of the L2-E2 interaction for the viral life cycle are discussed. ..
- Ebrahimi H, Donaldson A. Release of yeast telomeres from the nuclear periphery is triggered by replication and maintained by suppression of Ku-mediated anchoring. Genes Dev. 2008;22:3363-74 pubmed publisher..In addition to maintaining genetic information, DNA replication may therefore regulate subnuclear organization of chromatin. ..
- Jowsey P, Doherty A, Rouse J. Human PTIP facilitates ATM-mediated activation of p53 and promotes cellular resistance to ionizing radiation. J Biol Chem. 2004;279:55562-9 pubmed..Lowering hPTIP levels also increased cellular sensitivity to IR, suggesting that this protein plays a critical role in maintaining genome stability. ..
- Hayes J, McMahon M. Molecular basis for the contribution of the antioxidant responsive element to cancer chemoprevention. Cancer Lett. 2001;174:103-13 pubmed..The role of protein kinases in transducing chemical stress signals to the bZIP factors that affect gene induction through the ARE is discussed. ..
- Anderson L, Perkins N. Regulation of RelA (p65) function by the large subunit of replication factor C. Mol Cell Biol. 2003;23:721-32 pubmed..Taken together, these observations suggest that, in addition to its previously described function in DNA replication and repair, RFC (p140) has an important role as a regulator of transcription and NF-kappaB activity. ..
- Conway C, McCulloch R, Ginger M, Robinson N, Browitt A, Barry J. Ku is important for telomere maintenance, but not for differential expression of telomeric VSG genes, in African trypanosomes. J Biol Chem. 2002;277:21269-77 pubmed publisher..The absence of Ku and the consequent great shortening of telomeres had no detectable influence either on the rate of VSG switching or on the silencing of the telomeric promoters of the VSG subset that is expressed in the tsetse fly...
- Eblaghie M, Song S, Kim J, Akita K, Tickle C, Jung H. Interactions between FGF and Wnt signals and Tbx3 gene expression in mammary gland initiation in mouse embryos. J Anat. 2004;205:1-13 pubmed..These data, taken together with previous findings, suggest a model in which Tbx3 expression is induced and maintained in early gland initiation by both Wnt and Fgf signalling through FGFR1. ..
- Rawlins E, White N, Jarman A. Echinoid limits R8 photoreceptor specification by inhibiting inappropriate EGF receptor signalling within R8 equivalence groups. Development. 2003;130:3715-24 pubmed..We suggest that echinoid acts to prevent a similar inductive outcome of Egfr signalling during R8 selection. ..
- Elliott D, Oghene K, Makarov G, Makarova O, Hargreave T, Chandley A, et al. Dynamic changes in the subnuclear organisation of pre-mRNA splicing proteins and RBM during human germ cell development. J Cell Sci. 1998;111 ( Pt 9):1255-65 pubmed..These results suggest dynamic and possibly multiple functions for RBM in germ cell development. ..
- Ayscough K, Eby J, Lila T, Dewar H, Kozminski K, Drubin D. Sla1p is a functionally modular component of the yeast cortical actin cytoskeleton required for correct localization of both Rho1p-GTPase and Sla2p, a protein with talin homology. Mol Biol Cell. 1999;10:1061-75 pubmed..A homologue of SLA1 was identified in Schizosaccharomyces pombe. Despite relatively low overall sequence homology, this gene was able to rescue the temperature sensitivity associated with a deletion of SLA1 in Saccharomyces cerevisiae. ..
- MacLaine N, Hupp T. The regulation of p53 by phosphorylation: a model for how distinct signals integrate into the p53 pathway. Aging (Albany NY). 2009;1:490-502 pubmed
- Kohl A, Clayton R, Weber F, Bridgen A, Randall R, Elliott R. Bunyamwera virus nonstructural protein NSs counteracts interferon regulatory factor 3-mediated induction of early cell death. J Virol. 2003;77:7999-8008 pubmed..These data suggest that the BUN NSs protein can delay cell death in the early stages of BUN infection by inhibiting IRF-3-mediated apoptosis...
- Mole S, Milligan S, Graham S. Human papillomavirus type 16 E2 protein transcriptionally activates the promoter of a key cellular splicing factor, SF2/ASF. J Virol. 2009;83:357-67 pubmed publisher..Such E2-mediated control during differentiation of infected epithelial cells may facilitate late capsid protein expression and completion of the virus life cycle. ..
- Muller B, Blackburn J, Feijoo C, Zhao X, Smythe C. DNA-activated protein kinase functions in a newly observed S phase checkpoint that links histone mRNA abundance with DNA replication. J Cell Biol. 2007;179:1385-98 pubmed
- Nunez F, Chipchase M, Clarke A, Melton D. Nucleotide excision repair gene (ERCC1) deficiency causes G(2) arrest in hepatocytes and a reduction in liver binucleation: the role of p53 and p21. FASEB J. 2000;14:1073-82 pubmed..We consider that the liver phenotype is a response to unrepaired endogenous DNA damage, which may reflect an additional non-NER-related function for the ERCC1 protein. ..
- McMahon M, Itoh K, Yamamoto M, Hayes J. Keap1-dependent proteasomal degradation of transcription factor Nrf2 contributes to the negative regulation of antioxidant response element-driven gene expression. J Biol Chem. 2003;278:21592-600 pubmed..These data suggest that Keap1 negatively regulates Nrf2 by both enhancing its rate of proteasomal degradation and altering its subcellular distribution. ..
- Munoz I, Hain K, Déclais A, Gardiner M, Toh G, Sanchez Pulido L, et al. Coordination of structure-specific nucleases by human SLX4/BTBD12 is required for DNA repair. Mol Cell. 2009;35:116-27 pubmed publisher..Depletion of SLX4 causes a decrease in DSB-induced homologous recombination. These data show that SLX4 is a regulator of structure-specific nucleases and that SLX4 and SLX1 are important regulators of genome stability in human cells. ..
- Rena G, Prescott A, Guo S, Cohen P, Unterman T. Roles of the forkhead in rhabdomyosarcoma (FKHR) phosphorylation sites in regulating 14-3-3 binding, transactivation and nuclear targetting. Biochem J. 2001;354:605-12 pubmed
- Hunter R. Aetiology of intersexuality in female (XX) pigs, with novel molecular interpretations. Mol Reprod Dev. 1996;45:392-402 pubmed..Despite this proposition, interactions between genes that prescribe functional testicular tissue, enhanced rates of gonadal development, and left-right asymmetries between the paired gonads now require systematic study...
- Browne G, Fardilha M, Oxenham S, Wu W, Helps N, da Cruz e Silva O, et al. SARP, a new alternatively spliced protein phosphatase 1 and DNA interacting protein. Biochem J. 2007;402:187-96 pubmed..The presence of a leucine zipper near the C-terminus of SARP1 and SARP2, and the binding of mammalian DNA to SARP2, suggests that SARP1 and SARP2 may be transcription factors or DNA-associated proteins that modulate gene expression. ..
- Ladomery M, Slight J, Mc Ghee S, Hastie N. Presence of WT1, the Wilm's tumor suppressor gene product, in nuclear poly(A)(+) ribonucleoprotein. J Biol Chem. 1999;274:36520-6 pubmed..Similar results were obtained from two cell lines expressing WT1, fetal kidneys (day E17), and transiently transfected cells, suggesting that the presence of WT1 protein in nuclear poly(A)(+) RNP is a general aspect of WT1 function. ..
- Panov K, Friedrich J, Zomerdijk J. A step subsequent to preinitiation complex assembly at the ribosomal RNA gene promoter is rate limiting for human RNA polymerase I-dependent transcription. Mol Cell Biol. 2001;21:2641-9 pubmed..This rate-limiting step in Pol I transcription is likely to be a major target in the regulation of rRNA gene expression. ..
- Connelly J, de Leau E, Leach D. Nucleolytic processing of a protein-bound DNA end by the E. coli SbcCD (MR) complex. DNA Repair (Amst). 2003;2:795-807 pubmed..These observations indicate a more complex biochemical action than has been assumed previously and argue that this class of protein has the potential to play a direct role in deprotecting protein-bound DNA ends in vivo. ..
- Wilkie G, Davis I. Drosophila wingless and pair-rule transcripts localize apically by dynein-mediated transport of RNA particles. Cell. 2001;105:209-19 pubmed..We propose that dynein-dependent movement of RNA particles is a widely deployed mechanism for mRNA localization. ..
- Kerr I, Wadsworth R, Blankenfeldt W, Staines A, White M, Naismith J. Overexpression, purification, crystallization and data collection of a single-stranded DNA-binding protein from Sulfolobus solfataricus. Acta Crystallogr D Biol Crystallogr. 2001;57:1290-2 pubmed..Smaller needle-like crystals diffract to about 2.0 A but are not twinned. Molecular-replacement attempts have failed owing to low identity with available search models. The structure will be determined by multiple-wavelength methods. ..
- Nasir L, Gault E, Campbell S, Veeramalai M, Gilbert D, McFarlane R, et al. Isolation and expression of the reverse transcriptase component of the Canis familiaris telomerase ribonucleoprotein (dogTERT). Gene. 2004;336:105-13 pubmed..This data will allow for further investigation of telomerase in canine malignancies as well as the development of the dog as a model system for human telomerase investigations. ..
- Kriaucionis S, Bird A. The major form of MeCP2 has a novel N-terminus generated by alternative splicing. Nucleic Acids Res. 2004;32:1818-23 pubmed..The presence of a previously unknown MeCP2 isoform has implications for the genetic screening of Rett syndrome patients and for studies of the functional significance of MeCP2. ..
- Chapman N, Webster G, Gillespie P, Wilson B, Crouch D, Perkins N. A novel form of the RelA nuclear factor kappaB subunit is induced by and forms a complex with the proto-oncogene c-Myc. Biochem J. 2002;366:459-69 pubmed
- Berglund F, Clarke P. hnRNP-U is a specific DNA-dependent protein kinase substrate phosphorylated in response to DNA double-strand breaks. Biochem Biophys Res Commun. 2009;381:59-64 pubmed publisher..We show that hnRNP-U is phosphorylated at Ser59 by DNA-PK in vitro and in cells in response to DNA double-strand breaks. Phosphorylation of hnRNP-U suggests novel functions for DNA-PK in the response to DNA damage. ..
- Deak M, Clifton A, Lucocq L, Alessi D. Mitogen- and stress-activated protein kinase-1 (MSK1) is directly activated by MAPK and SAPK2/p38, and may mediate activation of CREB. EMBO J. 1998;17:4426-41 pubmed..These findings, together with other observations, suggest that MSK1 may mediate the growth-factor and stress-induced activation of CREB. ..